Cellular reprogramming最新文献

{"title":"The Role of Zinc in Bone Mesenchymal Stem Cell Differentiation.","authors":"Huiyun Li,&nbsp;Muzhe Li,&nbsp;Xun Ran,&nbsp;Juncheng Cui,&nbsp;Fu Wei,&nbsp;Guoliang Yi,&nbsp;Wei Chen,&nbsp;Xuling Luo,&nbsp;Zhiwei Chen","doi":"10.1089/cell.2021.0137","DOIUrl":"https://doi.org/10.1089/cell.2021.0137","url":null,"abstract":"<p><p>Zinc is an essential trace element for bone growth and bone homeostasis in the human body. Bone mesenchymal stem cells (BMSCs) are multipotent progenitors existing in the bone marrow stroma with the capability of differentiating along multiple lineage pathways. Zinc plays a paramount role in BMSCs, which can be spurred differentiating into osteoblasts, chondrocytes, or adipocytes, and modulates the formation and activity of osteoclasts. The expression of related genes also changed during the differentiation of various cell phenotypes. Based on the important role of zinc in BMSC differentiation, using zinc as a therapeutic approach for bone remodeling will be a promising method. This review explores the role of zinc ion in the differentiation of BMSCs into various cell phenotypes and outlines the existing research on their molecular mechanism.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 2","pages":"80-94"},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39790734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"General Control Nonrepressed Protein 5 Modulates Odontogenic Differentiation Through NF-κB Pathway in Tumor Necrosis Factor-α-Mediated Impaired Human Dental Pulp Stem Cells.","authors":"Jingwen Xiao,&nbsp;Ya Zheng,&nbsp;Wei Zhang,&nbsp;Ye Zhang,&nbsp;Peipei Cao,&nbsp;Yi Liang,&nbsp;Liuliu Bao,&nbsp;Suping Shi,&nbsp;Xingmei Feng","doi":"10.1089/cell.2021.0113","DOIUrl":"https://doi.org/10.1089/cell.2021.0113","url":null,"abstract":"<p><p>Dental pulp stem cells (DPSCs) from pulpitis patients showed defective osteogenic differentiation. However, as the most well-studied histone acetyltransferase, the impaired general control nonrepressed protein 5 (GCN5) plays essential roles in various developmental processes. The aim of this study was to investigate the effect of GCN5 on DPSCs odontogenic differentiation. The healthy dental pulp tissues were obtained from the extracted impacted third molar of patients with the informed consent. DPSCs were treated with a high concentration of tumor necrosis factor-alpha (TNF-α) (100 ng/mL) and odontogenic differentiation-related gene and GCN5 protein level by Western blot analysis. Proliferation of the DPSCs was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Immunofluorescence staining detected GCN5 and NF-κB signaling for p-p65. The mechanism of GCN5 regulating odontogenic differentiation of DPSCs was determined by small interfering RNA analysis. Our data suggested that TNF-α can significantly reduce mineralization and the expression of dentin matrix acidic phosphoprotein 1 and dentin sialophosphoprotein at higher concentration (100 ng/mL). Meanwhile, it showed that the inflammation in microenvironment resulted in a downregulation of GCN5 expression and GCN5 knockdown caused decreased odontogenic differentiation of DPSCs was also found. In addition, the knockdown of GCN5 increased the expression of phosphorylation of p65, thus activating NF-κB pathway of DPSCs. Meanwhile, NF-κB pathway inhibitor pyrrolidinedithiocarbamic acid reversed the siGCN5 decreased odontogenic differentiation of DPSCs. Altogether, our findings indicated that in inflammatory microenvironments GCN5 plays a protective role in pulpitis impaired odontogenic differentiation of DPSCs by activating NF-κB pathway, which may provide a potential approach to dentin regeneration.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 2","pages":"95-104"},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39929007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Progress and Prospects in Rabbit Cloning.","authors":"Wenbin Cao,&nbsp;Jinpeng Zhao,&nbsp;Pengxiang Qu,&nbsp;Enqi Liu","doi":"10.1089/cell.2021.0090","DOIUrl":"https://doi.org/10.1089/cell.2021.0090","url":null,"abstract":"<p><p>Somatic cell nuclear transfer (SCNT) shows great value in the generation of transgenic animals, protection of endangered animals, and stem cell therapy. The combination of SCNT and gene editing has produced a variety of genetically modified animals for life science and medical research. Rabbits have unique advantages as transgenic bioreactors and human disease models; however, the low SCNT efficiency severely impedes the application of this technology. The difficulty in SCNT may be attributable to the abnormal reprogramming of somatic cells in rabbits. This review focuses on the abnormal reprogramming of cloned mammalian embryos and evaluates the progress and prospects of rabbit somatic cell cloning.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 2","pages":"63-70"},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39925510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Sets of Compound Complex Driving for High Efficiency of Nonintegration Reprogramming of Human Fibroblasts.","authors":"Xiangyi Lin, Cuiping Rong, Shouhai Wu","doi":"10.1089/cell.2021.0143","DOIUrl":"https://doi.org/10.1089/cell.2021.0143","url":null,"abstract":"Currently, plentiful chemical-assisted methods have been applied for mouse induced pluripotent stem cells (iPSCs). It has been reported that small-molecule compounds can only reprogram mouse embryonic fibroblasts into mouse chemically induced pluripotent stem cells (mouse CiPSCs). However, human CiPSCs have not been reported. Therefore, it is still necessary to search for safer chemically assisted human pluripotent stem cells, which might realize the potential of human iPSCs. Here, we developed two sets of chemical cocktails to greatly improve the induction efficiency of human nonintegrated iPSCs, including the 4 compound mixture (4M) and the 5 compound mixture (4MI). These two sets of complex driving strategies might greatly improve the reprogramming efficiency to generate integration-free iPSCs.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47882629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming Stars #5: Regeneration, a Natural Reprogramming Process-An Interview with Dr. Nicholas Leigh.","authors":"Nicholas D Leigh,&nbsp;Carlos-Filipe Pereira","doi":"10.1089/cell.2022.29055.nl","DOIUrl":"https://doi.org/10.1089/cell.2022.29055.nl","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1","pages":"2-8"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39899218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment of Reviewers 2021.","authors":"","doi":"10.1089/cell.2022.29059.ack","DOIUrl":"https://doi.org/10.1089/cell.2022.29059.ack","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1 1","pages":"1"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45672832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapamycin Treatment Is Beneficial for the Generation of Rabbit-Induced Pluripotent Stem-Like Cells.","authors":"Xuan Ren,&nbsp;Wei Lei,&nbsp;Shihai Huang,&nbsp;Deshun Shi,&nbsp;Xiangping Li","doi":"10.1089/cell.2021.0128","DOIUrl":"https://doi.org/10.1089/cell.2021.0128","url":null,"abstract":"<p><p>Autophagy could promote the generation of induced pluripotency stem cells (iPSCs) in humans and mice. However, little was known whether it had similar effects in other species, the detailed mechanism and the features of formed iPSC colonies were also not clear. In this study, we first established the doxycycline (DOX)-inducible tetO lentiviral vector system suitable for the generation of rabbit iPSCs. Rapamycin, a mechanistic target of rapamycin (mTOR) inhibitor, was added during rabbit embryonic fibroblasts induction to improve the autophagy level. The colony formation efficiency and the expression of autophagy- and pluripotent-related genes were detected. The results showed that the established DOX-inducible tetO lentiviral system was successfully used to induce rabbit iPS-like cells. Compared with the untreated group, the number of alkaline phosphatase (AP)-positive colonies was increased 5.5-fold, when 0.5 nM rapamycin was added on days 1-3 after transduction, the colony morphology was improved and the iPS-like cells could be passaged >10 generations. The expression of autophagy-related genes (ATG), <i>ATG5</i>, <i>ATG7</i>, <i>LC3</i>, and <i>ULK1</i> was increased with different patterns during the induction process, expression of <i>OCT4</i>, <i>SOX2</i>, and <i>KLF4</i> significantly increased (<i>p</i> < 0.05). The mentioned results indicate that rapamycin treatment is beneficial for the generation of rabbit iPSCs by regulating autophagy and pluripotency-related gene expression.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1","pages":"48-55"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39864078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maclurin Promotes the Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Regulating miR-203a-3p/Smad1.","authors":"Jiechen Kong,&nbsp;Xianxi Zhou,&nbsp;Jianghua Lu,&nbsp;Qianting Han,&nbsp;Xiyan Ouyang,&nbsp;Dongfeng Chen,&nbsp;Aijun Liu","doi":"10.1089/cell.2021.0122","DOIUrl":"https://doi.org/10.1089/cell.2021.0122","url":null,"abstract":"<p><p>Bone marrow mesenchymal stem cells (BMSCs) differentiate into chondrocytes under appropriate conditions, providing a method for the treatment of bone- and joint-related diseases. Previously, we found that mulberry (<i>Morus nigra</i>) promoted the chondrogenic differentiation of BMSCs. Although the mechanism of action and active ingredients remain unknown, several studies describe the involvement of micro-RNAs. We obtained BMSCs from the bone marrow of Sprague Dawley rats. Cell Counting Kit-8 assays showed that maclurin (25 μg/mL) treatment was not toxic to BMSCs, and compared with untreated controls, maclurin upregulated Sox9 and Col2a expression. Quantitative-PCR revealed that miR-203a-3p levels decreased significantly during chondrogenic differentiation of BMSCs promoted by maclurin. Compared with treatment with an miR-203a-3p inhibitor, miR-203a-3p mimic inhibited expression of Sox9 and Col2a as evidenced by immunofluorescence staining and Western blotting. <i>Smad1</i> was identified as a key target gene of miR-203a-3p according to biological-prediction software, and miR-203a-3p negatively regulated its transcription and translation in the dual-luciferase reporter gene assay and Western blotting. Sox9 and Col2a expression was downregulated following transfection of short interfering Smad1 (siSmad1) plasmids into BMSCs. We elucidated how maclurin promotes the chondrogenic differentiation of BMSCs by regulating miR-203a-3p/Smad1, which provides a strategy for future exploration of osteoarthritis therapy through cell transplantation.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1","pages":"9-20"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39935475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency Comparison of Direct Reprogramming CD34⁺ Cells into Cardiomyocytes Using Cardiomyocyte Differentiation Medium vs MicroRNA-1.","authors":"Andrianto,&nbsp;Budi Susetyo Pikir,&nbsp;Ferdiansyah,&nbsp;Tinton Pristianto,&nbsp;Hanestya Oky Hermawan,&nbsp;Bagas Setiawan Ihsan Zaini,&nbsp;Akbar Reza Muhammad","doi":"10.1089/cell.2021.0075","DOIUrl":"https://doi.org/10.1089/cell.2021.0075","url":null,"abstract":"<p><p>The development of a direct reprogramming method to provide cell availability for regenerative therapy has led to a lot of studies. However, the search for appropriate cell sources and methods is still being carried out until now. Direct reprogramming using microRNA-1 (miR-1) is an option to obtain cardiomyocytes from other cells because miR-1 has evidence to play a role in the development of cardiac muscle cells in the embryo. This study aimed to compare the direct reprogramming efficiency of CD34⁺ cells from peripheral blood into cardiomyocytes between cardiomyocyte differentiation medium and miR-1. CD34⁺ cells from peripheral blood isolation and expansion process was conducted for 7 days using magnetic-activated cell sorting. Cardiomyocyte differentiation medium added in P1 group and transfection of miR-1 in P2 group of cell culture. Cardiac troponin immunocytochemistry staining and measurement were done on the fifth day after cell culture treatment. Cardiac troponin expression was observed higher in the P2 group (median 31.34) compared to the P1 group (median 21.06) (<i>p</i> = 0.000). The efficiency of direct reprogramming of CD34⁺ cells into cardiomyocytes with cardiomyocyte differentiation medium was 13%-21% and with miR-1 transfection was 31%-32%. Both the addition of miR-1 and cardiomyocyte differentiation medium could directly reprogram CD34⁺ cells into cardiomyocytes. The efficiency of miR-1 in reprogramming CD34⁺ cells into cardiomyocytes is superior to cardiomyocytes differentiation medium.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1","pages":"21-25"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39731649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Specific Regulatory Motifs Network in SARS-CoV-2-Infected Caco-2 Cell Line, as a Model of Gastrointestinal Infections.","authors":"Sepideh Ghani,&nbsp;Sima Kalantari,&nbsp;Seyed Amir Mirmotalebisohi,&nbsp;Marzieh Sameni,&nbsp;Hossein Poursheykhi,&nbsp;Sadaf Dadashkhan,&nbsp;Maryam Abbasi,&nbsp;Hakimeh Zali","doi":"10.1089/cell.2021.0055","DOIUrl":"https://doi.org/10.1089/cell.2021.0055","url":null,"abstract":"<p><p>The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was primarily noted as a respiratory pathogen, but later clinical reports highlighted its extrapulmonary effects particularly on the gastrointestinal (GI) tract. The aim of the current study was the prediction of crucial genes associated with the regulatory network motifs, probably responsible for the SARS-CoV-2 effects on the GI tract. The data were obtained from a published study on the effect of SARS-CoV-2 on the Caco-2 (colon carcinoma) cell line. We used transcription factors-microRNA-gene interaction databases to find the key regulatory molecules, then analyzed the data using the FANMOD software for detection of the crucial regulatory motifs. Cytoscape software was then used to construct and analyze the regulatory network of these motifs and identify their crucial genes. Finally, GEPIA2 (Gene Expression Profiling Interactive Analysis 2) and UALCAN datasets were used to evaluate the possible relationship between crucial genes and colon cancer development. Using bioinformatics tools, we demonstrated one 3edge feed-forward loop motifs and recognized 10 crucial genes in relationship with Caco-2 cell infected by SARS-CoV-2, including <i>SP1</i>, <i>TSC22D2</i>, <i>POU2F1</i>, <i>REST</i>, <i>NFIC</i>, <i>CHD7</i>, <i>E2F1</i>, <i>CEBPA</i>, <i>TCF7L2</i>, and <i>TSC22D1</i>. The box plot analysis indicated the significant overexpression of <i>CEBPA</i> in colon cancer compared to normal colon tissues, while it was in contrast with the results of stage plot. However, the overall survival analysis indicated that high expression of <i>CEBPA</i> has positive effect on colon cancer patient survivability, verifying the results of <i>CEBPA</i> stage plot. We predict that the SARS-CoV-2 GI infections may cause a serious risk in colon cancer patients. However, further experimental studies are required.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1","pages":"26-37"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39875332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}