Cellular reprogramming最新文献_第9页

Advances in Understanding the Roles of Mesenchymal Stem Cells in Lung Cancer. 间充质干细胞在肺癌中的作用研究进展。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-02-01 DOI: 10.1089/cell.2022.0133

Wenli Ding, Kexin Zhang, Qinying Li, Linfei Xu, Yanhui Ma, Fang Han, Liang Zhu, Xiaodong Sun

引用次数: 0

Bcl11b and Atoh8 Coordinate Cellular Plasticity for Reprogramming and Transformation. Bcl11b和Atoh8协调细胞重编程和转化的可塑性。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 Epub Date: 2022-11-21 DOI: 10.1089/cell.2022.0128

Mo-Fan Huang, Rachel Shoemaker, Dung-Fang Lee

引用次数: 1

Call for Special Issue Papers: Cellular Reprogramming 25th Anniversary Deadline for Manuscript Submission: April 30, 2023. 特刊论文征集:细胞重编程25周年论文提交截止日期:2023年4月30日。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.29073.cfp

Carlos-Filipe Pereira

引用次数: 0

Direct Reprogramming Retains Aging Signatures That Are Critical to Reveal Parkinson's Disease-Associated Autophagy Phenotypes. 直接重编程保留衰老特征是揭示帕金森病相关自噬表型的关键

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0127

Elezabeth Stephen, Heather Mortiboys

引用次数: 0

Fluorescent Reporters Distinguish Stem Cell Colony Subtypes During Somatic Cell Reprogramming. 在体细胞重编程过程中用荧光报告区分干细胞集落亚型

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 Epub Date: 2022-11-03 DOI: 10.1089/cell.2022.0071

Alexandra Moauro, Robin E Kruger, Daniel O'Hagan, Amy Ralston

{"title":"Fluorescent Reporters Distinguish Stem Cell Colony Subtypes During Somatic Cell Reprogramming.","authors":"Alexandra Moauro, Robin E Kruger, Daniel O'Hagan, Amy Ralston","doi":"10.1089/cell.2022.0071","DOIUrl":"10.1089/cell.2022.0071","url":null,"abstract":"Somatic cell reprogramming was first developed to create induced pluripotent stem (iPS) cells. Since that time, the highly dynamic and heterogeneous nature of the reprogramming process has come to be appreciated. Remarkably, a distinct type of stem cell, called induced extraembryonic endoderm (iXEN) stem cell, is also formed during reprogramming of mouse somatic cells by ectopic expression of the transcription factors, OCT4, SOX2, KLF4, and MYC (OSKM). The mechanisms leading somatic cells to adopt differing stem cell fates are challenging to resolve given that formation of either stem cell type is slow, stochastic, and rare. For these reasons, fluorescent gene expression reporters have provided an invaluable tool for revealing the path from the somatic state to pluripotency. However, no such reporters have been established for comparable studies of iXEN cell formation. In this study, we examined the expression of multiple fluorescent reporters, including Nanog, Oct4, and the endodermal genes, Gata4 and Gata6-alone and in combination, during reprogramming. We show that only simultaneous evaluation of Nanog and Gata4 reliably distinguishes iPS and iXEN cell colonies during reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 6","pages":"353-362"},"PeriodicalIF":1.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/1e/cell.2022.0071.PMC9805857.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9080971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Tumor Microenvironment Reprograms Immune Cells. 肿瘤微环境重编程免疫细胞。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0047

Handi Cao, Sanxing Gao, Ritika Jogani, Ryohichi Sugimura

引用次数: 3

Reprogramming Stars #9: Spacing Out Cellular Reprogramming-An Interview with Dr. Valentina Fossati. 重编程之星 #9：间隔细胞重编程--专访瓦伦蒂娜-福萨蒂博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 Epub Date: 2022-11-21 DOI: 10.1089/cell.2022.29074.vf

Valentina Fossati, Carlos-Filipe Pereira

引用次数: 0

The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus. 间充质干细胞治疗糖尿病的潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0039

Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun

{"title":"The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus.","authors":"Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun","doi":"10.1089/cell.2022.0039","DOIUrl":"https://doi.org/10.1089/cell.2022.0039","url":null,"abstract":"Mesenchymal stem cells (MSCs) exist in many tissues and can differentiate into cells of multiple lineages, such as adipocytes, osteoblasts, or chondrocytes. MSC administration has demonstrated therapeutic potential in various degenerative and inflammatory diseases (e.g., graft-vs.-host disease, multiple sclerosis, Crohn's disease, organ fibrosis, and diabetes mellitus [DM]). The mechanisms involved in the therapeutic effects of MSCs are multifaceted. Generally, implanted MSCs can migrate to sites of injury, where they establish an anti-inflammatory and regenerative microenvironment in damaged tissues. In addition, MSCs can modulate innate and adaptive immune responses through immunosuppressive mechanisms that involve immune cells, inflammatory cytokines, chemokines, and immunomodulatory factors. DM has a high prevalence worldwide; it also contributes to a high rate of mortality worldwide. MSCs offer a promising therapeutic agent to prevent or repair damage from DM and diabetic complications through properties such as multilineage differentiation, homing, promotion of angiogenesis, and immunomodulation (e.g., prevention of oxidative stress, fibrosis, and cell death). In this study, we review current findings regarding the immunomodulatory and regenerative mechanisms of MSCs, as well as their therapeutic applications in DM and DM-related complications.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 6","pages":"329-342"},"PeriodicalIF":1.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10574455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion. 用Dorsomorphin同源物1化学替代Noggin实现低成本的直接神经元转换。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 DOI: 10.1089/cell.2021.0200

Lena Böhnke, Lucia Zhou-Yang, Silvia Pelucchi, Flora Kogler, Daniela Frantal, Florian Schön, Stina Lagerström, Oliver Borgogno, Jennifer Baltazar, Joseph R Herdy, Sarah Kittel-Schneider, Michaela Defrancesco, Jerome Mertens

{"title":"Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion.","authors":"Lena Böhnke, Lucia Zhou-Yang, Silvia Pelucchi, Flora Kogler, Daniela Frantal, Florian Schön, Stina Lagerström, Oliver Borgogno, Jennifer Baltazar, Joseph R Herdy, Sarah Kittel-Schneider, Michaela Defrancesco, Jerome Mertens","doi":"10.1089/cell.2021.0200","DOIUrl":"https://doi.org/10.1089/cell.2021.0200","url":null,"abstract":"The direct conversion of adult human skin fibroblasts (FBs) into induced neurons (iNs) represents a useful technology to generate donor-specific adult-like human neurons. Disease modeling studies rely on the consistently efficient conversion of relatively large cohorts of FBs. Despite the identification of several small molecular enhancers, high-yield protocols still demand addition of recombinant Noggin. To identify a replacement to circumvent the technical and economic challenges associated with Noggin, we assessed dynamic gene expression trajectories of transforming growth factor-β signaling during FB-to-iN conversion. We identified ALK2 (ACVR1) of the bone morphogenic protein branch to possess the highest initial transcript abundance in FBs and the steepest decline during successful neuronal conversion. We thus assessed the efficacy of dorsomorphin homolog 1 (DMH1), a highly selective ALK2-inhibitor, for its potential to replace Noggin. Conversion media containing DMH1 (+DMH1) indeed enhanced conversion efficiencies over basic SMAD inhibition (tSMADi), yielding similar βIII-tubulin (TUBB3) purities as conversion media containing Noggin (+Noggin). Furthermore, +DMH1 induced high yields of iNs with clear neuronal morphologies that are positive for the mature neuronal marker NeuN. Validation of +DMH1 for iN conversion of FBs from 15 adult human donors further demonstrates that Noggin-free conversion consistently yields iN cultures that display high βIII-tubulin numbers with synaptic structures and basic spontaneous neuronal activity at a third of the cost.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 5","pages":"304-313"},"PeriodicalIF":1.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9905634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct Reprogramming of Different Cell Lineages into Pancreatic β-Like Cells. 将不同细胞系直接重新编程为胰腺β样细胞。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 Epub Date: 2022-07-15 DOI: 10.1089/cell.2022.0048

Jonathan L Colarusso, Qiao Zhou

引用次数: 0