Cellular reprogramming最新文献_第9页

The Tumor Microenvironment Reprograms Immune Cells. 肿瘤微环境重编程免疫细胞。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0047

Handi Cao, Sanxing Gao, Ritika Jogani, Ryohichi Sugimura

引用次数: 3

Reprogramming Stars #9: Spacing Out Cellular Reprogramming-An Interview with Dr. Valentina Fossati. 重编程之星 #9：间隔细胞重编程--专访瓦伦蒂娜-福萨蒂博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 Epub Date: 2022-11-21 DOI: 10.1089/cell.2022.29074.vf

Valentina Fossati, Carlos-Filipe Pereira

引用次数: 0

The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus. 间充质干细胞治疗糖尿病的潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0039

Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun

{"title":"The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus.","authors":"Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun","doi":"10.1089/cell.2022.0039","DOIUrl":"https://doi.org/10.1089/cell.2022.0039","url":null,"abstract":"Mesenchymal stem cells (MSCs) exist in many tissues and can differentiate into cells of multiple lineages, such as adipocytes, osteoblasts, or chondrocytes. MSC administration has demonstrated therapeutic potential in various degenerative and inflammatory diseases (e.g., graft-vs.-host disease, multiple sclerosis, Crohn's disease, organ fibrosis, and diabetes mellitus [DM]). The mechanisms involved in the therapeutic effects of MSCs are multifaceted. Generally, implanted MSCs can migrate to sites of injury, where they establish an anti-inflammatory and regenerative microenvironment in damaged tissues. In addition, MSCs can modulate innate and adaptive immune responses through immunosuppressive mechanisms that involve immune cells, inflammatory cytokines, chemokines, and immunomodulatory factors. DM has a high prevalence worldwide; it also contributes to a high rate of mortality worldwide. MSCs offer a promising therapeutic agent to prevent or repair damage from DM and diabetic complications through properties such as multilineage differentiation, homing, promotion of angiogenesis, and immunomodulation (e.g., prevention of oxidative stress, fibrosis, and cell death). In this study, we review current findings regarding the immunomodulatory and regenerative mechanisms of MSCs, as well as their therapeutic applications in DM and DM-related complications.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 6","pages":"329-342"},"PeriodicalIF":1.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10574455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion. 用Dorsomorphin同源物1化学替代Noggin实现低成本的直接神经元转换。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 DOI: 10.1089/cell.2021.0200

Lena Böhnke, Lucia Zhou-Yang, Silvia Pelucchi, Flora Kogler, Daniela Frantal, Florian Schön, Stina Lagerström, Oliver Borgogno, Jennifer Baltazar, Joseph R Herdy, Sarah Kittel-Schneider, Michaela Defrancesco, Jerome Mertens

{"title":"Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion.","authors":"Lena Böhnke, Lucia Zhou-Yang, Silvia Pelucchi, Flora Kogler, Daniela Frantal, Florian Schön, Stina Lagerström, Oliver Borgogno, Jennifer Baltazar, Joseph R Herdy, Sarah Kittel-Schneider, Michaela Defrancesco, Jerome Mertens","doi":"10.1089/cell.2021.0200","DOIUrl":"https://doi.org/10.1089/cell.2021.0200","url":null,"abstract":"The direct conversion of adult human skin fibroblasts (FBs) into induced neurons (iNs) represents a useful technology to generate donor-specific adult-like human neurons. Disease modeling studies rely on the consistently efficient conversion of relatively large cohorts of FBs. Despite the identification of several small molecular enhancers, high-yield protocols still demand addition of recombinant Noggin. To identify a replacement to circumvent the technical and economic challenges associated with Noggin, we assessed dynamic gene expression trajectories of transforming growth factor-β signaling during FB-to-iN conversion. We identified ALK2 (ACVR1) of the bone morphogenic protein branch to possess the highest initial transcript abundance in FBs and the steepest decline during successful neuronal conversion. We thus assessed the efficacy of dorsomorphin homolog 1 (DMH1), a highly selective ALK2-inhibitor, for its potential to replace Noggin. Conversion media containing DMH1 (+DMH1) indeed enhanced conversion efficiencies over basic SMAD inhibition (tSMADi), yielding similar βIII-tubulin (TUBB3) purities as conversion media containing Noggin (+Noggin). Furthermore, +DMH1 induced high yields of iNs with clear neuronal morphologies that are positive for the mature neuronal marker NeuN. Validation of +DMH1 for iN conversion of FBs from 15 adult human donors further demonstrates that Noggin-free conversion consistently yields iN cultures that display high βIII-tubulin numbers with synaptic structures and basic spontaneous neuronal activity at a third of the cost.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 5","pages":"304-313"},"PeriodicalIF":1.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9905634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct Reprogramming of Different Cell Lineages into Pancreatic β-Like Cells. 将不同细胞系直接重新编程为胰腺β样细胞。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 Epub Date: 2022-07-15 DOI: 10.1089/cell.2022.0048

Jonathan L Colarusso, Qiao Zhou

引用次数: 0

Optimal Stage for Cryotop Vitrification of Porcine Embryos. 猪胚胎冷冻玻璃化的最佳阶段。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-06-01 DOI: 10.1089/cell.2022.0001

Hongxia Xu, Xuguang Wang, Ruixin Tao, Jiaying Bi, Xu He, Fuquan Zhu, Ke-wei Liu, Yinxue Xu, Juan Li

{"title":"Optimal Stage for Cryotop Vitrification of Porcine Embryos.","authors":"Hongxia Xu, Xuguang Wang, Ruixin Tao, Jiaying Bi, Xu He, Fuquan Zhu, Ke-wei Liu, Yinxue Xu, Juan Li","doi":"10.1089/cell.2022.0001","DOIUrl":"https://doi.org/10.1089/cell.2022.0001","url":null,"abstract":"Different development stages of porcine embryos have different tolerance to low temperature. Therefore, we took the porcine embryos after parthenogenetic activation (PA) as the model, to explore the optimal development stage for vitrification during morula (D4), early blastocyst (D5), and expanded blastocyst (D6) after PA (D0). Embryos were observed with microscope and analyzed by different staining after cryo-recovery for 24 hours. The quality of embryos was damaged after vitrification, including embryonic nuclei, DNA, cytoskeleton, and organelles. The re-expansion rate at 24 hours of D5 embryos was significantly higher than those of D4 and D6 embryos (D5 vs. D4 vs. D6, 27.620 ± 0.041 vs. 7.809 ± 0.027 vs. 13.970 ± 0.032, p < 0.05). Therefore, D5 embryos were selected as research objects to explore the effect of vitrification on lipid in vitrified embryos. The results showed that the expression levels of perilipin PLIN3 messenger RNA (mRNA) and triacylglycerol synthesis-related genes AGPAT1 and DGAT mRNA are significantly reduced (p < 0.05). Vitrification affected lipid synthesis, which might have an irreversible impact on embryonic development. In conclusion, our data demonstrated that the optimal stage of vitrification was D5 for early blastocysts.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 3 1","pages":"132-141"},"PeriodicalIF":1.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43794724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preconditioning with Trehalose Protects the Bone Marrow-Derived Mesenchymal Stem Cells Under Oxidative Stress and Enhances the Stem Cell-Based Therapy for Cerebral Ischemic Stroke. 海藻糖预处理对氧化应激下骨髓间充质干细胞的保护作用及对缺血性脑卒中干细胞治疗的促进作用

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-05-31 DOI: 10.1089/cell.2022.0037

Bing Shu, Jingsong Wan, Xiang Li, Raynald Liu, Cheng-shi Xu, Yihua An, Jingcao Chen

{"title":"Preconditioning with Trehalose Protects the Bone Marrow-Derived Mesenchymal Stem Cells Under Oxidative Stress and Enhances the Stem Cell-Based Therapy for Cerebral Ischemic Stroke.","authors":"Bing Shu, Jingsong Wan, Xiang Li, Raynald Liu, Cheng-shi Xu, Yihua An, Jingcao Chen","doi":"10.1089/cell.2022.0037","DOIUrl":"https://doi.org/10.1089/cell.2022.0037","url":null,"abstract":"Bone marrow-derived mesenchymal stem cell (BMSC) transplantation has emerged as a potential treatment for ischemic stroke. Preconditioning with pharmacological agents before cell transplantation has been shown to increase the efficiency of cell therapy. In this study, trehalose (Tre), an autophagy inducer, was used as a pharmacological agent to treat BMSCs, and the neuroprotective effect of BMSCs preconditioned with Tre on cerebral ischemia was assessed. BMSCs were treated in vitro with different concentrations of Tre. Immunofluorescence staining of LC3B was performed to detect autophagy, and Western blotting for LC3, Beclin1, p-AMPK, and p-mTOR was performed. Flow cytometry and Western blotting analysis were performed to measure cell apoptosis in the presence of hydrogen peroxide (H2O2). Enzyme-linked immunosorbent assay was used to test the secretion levels of neurotrophic factors. An in vivo ischemia/reperfusion model was generated by middle cerebral artery occlusion in male Sprague Dawley rats, and Tre-preconditioned BMSCs were administered intralesionally 24 hours after ischemic injury. Histopathological examination and neurological function studies were conducted. In vitro, Tre promotes autophagy of BMSCs through the activation of the AMPK signal pathway. Tre protected BMSCs from H2O2-induced cell viability reduction and apoptosis. Moreover, Tre pretreatment increased the secretion of brain-derived neurotrophic factor, vascular endothelial growth factor, and hepatocyte growth factor. In vivo, preconditioning with Tre could further enhance the survival of BMSCs, reduce infarct size, alleviate cell apoptosis, abate vessel decrease, and ultimately improve functional recovery. Our study indicates that Tre can enhance the survival of BMSCs under oxidative stress and enhance BMSC-based treatment of ischemia/reperfusion injury.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41848320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In Vitro Induction of Human Dental Pulp Stem Cells to Lymphatic Endothelial Cells. 人牙髓干细胞体外诱导淋巴内皮细胞的研究。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-05-13 DOI: 10.1089/cell.2021.0106

Shuqun Qi, L. Ye, Liru Hu, Jian Pan

引用次数: 1

An Alternative Way to Improve Mammalian Embryo Development In Vitro: Culture of Zona Pellucida-Free Embryos. 一种改善哺乳动物胚胎体外发育的替代方法：培养无Pellucida带胚胎。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-05-03 DOI: 10.1089/cell.2022.0012

Sarah Madani, Z. Machaty, G. Vajta

引用次数: 3

Effects of Trichostatin A on the Timing of the First Cleavage and In Vitro Developmental Potential of Bovine Somatic Cell Nuclear Transfer Embryos. 曲霉菌素A对牛体细胞核移植胚胎第一次切割时机和体外发育潜力的影响。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-04-11 DOI: 10.1089/cell.2022.0003

S. Akagi, K. Matsukawa

{"title":"Effects of Trichostatin A on the Timing of the First Cleavage and In Vitro Developmental Potential of Bovine Somatic Cell Nuclear Transfer Embryos.","authors":"S. Akagi, K. Matsukawa","doi":"10.1089/cell.2022.0003","DOIUrl":"https://doi.org/10.1089/cell.2022.0003","url":null,"abstract":"This study examined the relationship between the timing of the first cleavage and in vitro development of bovine somatic cell nuclear transfer (SCNT) embryos treated with trichostatin A (TSA). SCNT embryos were visually assessed at 22, 26, and 48 hours after activation. Each embryo with two or more distinct blastomeres was transferred into a microwell and cultured until day 7. Irrespective of TSA treatment, approximately half of the cleaved embryos were observed at 22 hours, and a significantly higher blastocyst formation rate was shown in the SCNT embryos cleaved at 22 hours than those cleaved at ≥26 hours. The blastocyst formation rate of TSA-treated embryos cleaved at 22 hours (80%) was slightly higher than that of the control embryos (70%). In addition, interferon-τ (IFN-τ) expression was significantly lower in control SCNT embryos and late-cleaving (>26 hours) TSA-treated embryos than in in vitro fertilized (IVF) embryos. However, a significant difference was not observed between TSA-treated SCNT embryos cleaved at 22 and 26 hours, and IVF embryos. These results suggest that TSA treatment has no influence on the timing of the first cleavage of SCNT embryos; however, it slightly improves the blastocyst formation rate and the expression level of IFN-τ in early-cleaving embryos.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41346897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0