Cellular reprogramming最新文献

Transformation of an Olfactory Placode-Derived Cell into One with Stem Cell Characteristics by Disrupting Epigenetic Barriers. 通过破坏表观遗传屏障将嗅觉基板衍生细胞转化为具有干细胞特征的细胞。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-07-14 DOI: 10.1177/21524971251359000

Ghazia Abbas, Rutesh Vyas, Joyce C Noble, Brian Lin, Robert P Lane

{"title":"Transformation of an Olfactory Placode-Derived Cell into One with Stem Cell Characteristics by Disrupting Epigenetic Barriers.","authors":"Ghazia Abbas, Rutesh Vyas, Joyce C Noble, Brian Lin, Robert P Lane","doi":"10.1177/21524971251359000","DOIUrl":"10.1177/21524971251359000","url":null,"abstract":"The mammalian olfactory neuronal lineage is regenerative, and accordingly, maintains a population of pluripotent cells that replenish olfactory sensory neurons and other olfactory cell types during the life of the animal. Moreover, in response to acute injury, the early transit amplifying cells along the olfactory sensory neuronal lineage are able to de-differentiate to shift resources in support of tissue restoration. In order to further explore plasticity of various cellular stages along the olfactory sensory neuronal lineage, we challenged the epigenetic stability of olfactory placode-derived cell lines that model immature olfactory sensory neuronal stages. We found that perturbation of the Ehmt2 chromatin modifier transformed the growth properties, morphology, and gene expression profiles toward states with several stem cell characteristics. This transformation was dependent on continued expression of the large T-antigen, and was enhanced by Sox2 over-expression. These findings may provide momentum for exploring inherent cellular plasticity within early cell types of the olfactory lineage, as well as potentially add to our knowledge of cellular reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Reprogramming-Restoring Function to Aged Cells. 精确重编程-恢复老化细胞的功能。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-09 DOI: 10.1089/cell.2025.0018

Arjun Jain, Yuuki Hosokawa, Kevin Joseph

引用次数: 0

Reprogramming of Different Cell Lineages into Functional β-Cell Substitutes. 不同细胞系重编程成功能性β-细胞替代品。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-09 DOI: 10.1089/cell.2024.0102

Anna A Dattoli, Yosip Kelemen, Xiaofeng Huang

引用次数: 0

Menstrual Blood-Derived Mesenchymal Stem Cells Improve Endometrial Receptivity in a Mouse Model of Embryonic Implantation Dysfunction. 经血源性间充质干细胞改善胚胎着床功能障碍小鼠模型的子宫内膜容受性。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-04-22 DOI: 10.1089/cell.2024.0071

Chao Ma, Yue Yi, Changji Guan

{"title":"Menstrual Blood-Derived Mesenchymal Stem Cells Improve Endometrial Receptivity in a Mouse Model of Embryonic Implantation Dysfunction.","authors":"Chao Ma, Yue Yi, Changji Guan","doi":"10.1089/cell.2024.0071","DOIUrl":"10.1089/cell.2024.0071","url":null,"abstract":"The decrease of endometrial receptivity leads to repeated implantation failure (RIF) during in vitro fertilization and embryo transfer. To explore the therapeutic potential of menstrual blood-derived mesenchymal stem cells (MenSCs) in addressing RIF, we established a murine model of embryonic implantation dysfunction using mifepristone. Subsequently, we administered MenSCs to these mice via tail vein injection and assessed their impact on the implantation and pregnancy rates of the affected mice. Furthermore, we conducted immunohistochemical staining on uterine tissues from these mice to examine the expression of endometrial receptivity markers, specifically vascular endothelial growth factor (VEGF)-A, HAND2, and HOXA10 following MenSCs transplantation. In parallel, we conducted in vitro studies to elucidate the molecular mechanisms of cell therapy by measuring the expression levels of VEGF-A, HAND2, and HOXA10 in endometrial stromal cells using real-time PCR and western blotting. In our mifepristone-induced mouse models, we observed a reduction in both pregnancy rates and implantation sites; however, these parameters were significantly improved after MenSCs transplantation. Similarly, the expression levels of VEGF-A, HAND2, and HOXA10 in the uterine tissues of the mifepristone group were diminished, but these levels were restored following MenSCs therapy. In vitro, after mifepristone treating, the expression of VEGF-A, HAND2, and HOXA10 decreased in endometrial stromal cells, but their expression increased after MenSCs coculture supernatant. In conclusion, these results demonstrated that MenSCs transplantation could increase endometrial receptivity by upregulating VEGF-A, HAND2, and HOXA10 expression. This study suggests MenSCs as a novel stem cell candidate in the treatment of RIF.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"124-133"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Locking the Fate: How PROX1 Represses Plasticity and Liver Cancer. 锁定命运：PROX1如何抑制可塑性和肝癌。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1089/cell.2025.0013

Seung-Won Lee, Jungsun Kim

引用次数: 0

Rejuvenation to the Heart: Overcoming Age-Related Metabolic Barriers in Direct Cardiac Reprogramming. 心脏再生：克服直接心脏重编程中与年龄相关的代谢障碍。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1089/cell.2025.0009

Pedro P Cunha, Mariana Lopes

引用次数: 0

Reprogramming Stars #22: Cellular Reprogramming as a Route for Decoding Neurodegenerative Disorders-An Interview with Dr. Janelle Drouin-Ouellet. 重编程之星#22：细胞重编程作为解码神经退行性疾病的途径——采访Janelle Drouin-Ouellet博士。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-05-20 DOI: 10.1089/cell.2025.77701.int

Janelle Drouin-Ouellet, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

Delivery Systems in Neuronal Direct Cell Reprogramming. 神经元直接细胞重编程中的传递系统。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1089/cell.2025.0008

Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H A M Fens, Massimiliano Caiazzo

{"title":"Delivery Systems in Neuronal Direct Cell Reprogramming.","authors":"Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H A M Fens, Massimiliano Caiazzo","doi":"10.1089/cell.2025.0008","DOIUrl":"10.1089/cell.2025.0008","url":null,"abstract":"Neuronal direct cell reprogramming approach allows direct conversion of somatic cells into neurons via forced expression of neuronal cell-lineage transcription factors (TFs). These so-called induced neuronal cells have significant potential as research tools and for therapeutic applications, such as in cell replacement therapy. However, the optimization of TF delivery strategies is crucial to reach clinical practice. In this review, we outlined the currently explored delivery technologies in neuronal direct cell reprogramming and their limitations and advantages. The first employed delivery strategies were mainly integrating viral systems, such as lentiviruses that exert consistently high transgene expression in most cell types. On the other hand, viral systems cause major safety concerns, including the risk for insertional mutagenesis and inflammation. More recently, several safer nonviral delivery systems have been investigated as well; however, these systems generally exert inferior reprogramming efficiency compared with viral systems. Emerging delivery technologies could provide new opportunities in the achievement of safe and effective delivery for neuronal direct cell reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"109-123"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases. 磷脂酶C δ 3及其在肿瘤疾病中的意义的新见解。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-12 DOI: 10.1089/cell.2025.0004

Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang

{"title":"Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases.","authors":"Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang","doi":"10.1089/cell.2025.0004","DOIUrl":"https://doi.org/10.1089/cell.2025.0004","url":null,"abstract":"The phospholipase C (PLC) family plays a crucial role in the construction of biomembranes, cell growth, and signal transduction. PLC regulates multiple cellular processes by generating bioactive molecules such as inositol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These products propagate and regulate cellular signaling via calcium (Ca2+) mobilization and activation of protein kinase C (PKC), other kinases, and ion channels. Recently, the function of PLC delta 3 (PLCδ3) has been arousing great interests in the basic research of neoplastic diseases. It is demonstrated to affect multiple parts of tumor progression and promote glycolysis reprogramming. However, currently there are no conclusive reports regarding the mechanism of PLCδ3-mediated tumor progression and its importance as a prognostic biomarker in specific neoplastic diseases. Therefore, the present article aimed to illustrate (1) the correlation between the function of phospholipases in PLC family and tumor progression; (2) the PLCδ3-mediated tumor progression, mainly focusing on the signal transduction and regulation; and (3) its potential mechanism and vital targets involved in multiple malignancies.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Old Habits Die Hard: DNA Methylation Patterns Persist in Experimental Transdifferentiation. 旧习惯难改：DNA甲基化模式在实验转分化中持续存在。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-12 DOI: 10.1089/cell.2025.0020

Alice E Lord, Leo J Dudley, Lynette Graver, Gabriella Ficz

引用次数: 0