Cellular reprogramming最新文献

Bidirectional Prime Editing: Combining Precision with Versatility for Genome Editing.

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-17 DOI: 10.1089/cell.2024.0075

Mahmood S Choudhery, Taqdees Arif, Ruhma Mahmood

引用次数: 0

Transplantation of Human Induced Pluripotent Stem Cell-Derived Airway Epithelia at Different Induction Stages into Nude Rat. 将不同诱导阶段的人类诱导多能干细胞衍生气道上皮细胞移植到裸鼠体内

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0054

Keisuke Mizuno, Hiroe Ohnishi, Yo Kishimoto, Hideaki Okuyama, Yoshitaka Kawai, Masayuki Kitano, Yasuyuki Hayashi, Koichi Omori

{"title":"Transplantation of Human Induced Pluripotent Stem Cell-Derived Airway Epithelia at Different Induction Stages into Nude Rat.","authors":"Keisuke Mizuno, Hiroe Ohnishi, Yo Kishimoto, Hideaki Okuyama, Yoshitaka Kawai, Masayuki Kitano, Yasuyuki Hayashi, Koichi Omori","doi":"10.1089/cell.2024.0054","DOIUrl":"10.1089/cell.2024.0054","url":null,"abstract":"Tracheal reconstruction is necessary in patients with large tracheal defects. Previously, artificial tracheae made of polypropylene and collagen sponge have been used clinically by our group. As a basic research aimed at promoting epithelialization for infection defense, we transplanted cell sheets of human induced pluripotent stem cell (hiPSC)-derived airway epithelial cells (iAECs) with artificial tracheae into tracheal defects of rats and confirmed their engraftment. In this study, we examined the difference in the cell engraftment between hiPSC-derived airway epithelial progenitor cells (iAEPCs) and iAECs. Cell sheets were collected on days 38, 45, and 56 of induction into iAECs, then transplanted into nude rats with tracheal defects along with the artificial trachea. Two weeks after transplantation, surviving human nuclear antigen (HNA)-positive epithelial cells were observed none of six rats in the 38-day group, two out of six in 45-day group, and five out of six in the 56-day group. The proportion of surviving HNA+ cells among the epithelial cells of 56-day group was significantly higher those of 38-day group. Differentiated iAECs are more suitable for the transplantation of hiPSCs into tracheal defects. Our findings propose the use of differentiated cells for improvement of engraftment efficiency.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"156-163"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming of Expanded Cord Blood-Derived CD34⁺ Cells from Umbilical Cord-Mesenchymal Stromal Cell Co-Culture to Generate Human-Induced Pluripotent Stem Cells. 从脐带-间质基质细胞共培养中对扩增的脐带血CD34+细胞进行重编程，以生成人类诱导多能干细胞。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0073

Fatin Fazrina Roslan, Yuexin Yu, Mengmeng Wang, Nurul Ain Nasim Mohd Yusof, Ghee Chien Ooi, Khong Lek Then, Kong Yong Then, Soon-Keng Cheong, Mohd Nor Azim Ab Patar, Jun Jie Tan

{"title":"Reprogramming of Expanded Cord Blood-Derived CD34+ Cells from Umbilical Cord-Mesenchymal Stromal Cell Co-Culture to Generate Human-Induced Pluripotent Stem Cells.","authors":"Fatin Fazrina Roslan, Yuexin Yu, Mengmeng Wang, Nurul Ain Nasim Mohd Yusof, Ghee Chien Ooi, Khong Lek Then, Kong Yong Then, Soon-Keng Cheong, Mohd Nor Azim Ab Patar, Jun Jie Tan","doi":"10.1089/cell.2024.0073","DOIUrl":"10.1089/cell.2024.0073","url":null,"abstract":"Cord blood (CB) is widely stored as a source of hematopoietic stem cells for potential future use, though its application for autologous purposes remains limited. Repurposing CB into human-induced pluripotent stem cells (hiPSCs) can broaden its utility beyond hematological conditions. This study investigated the effects of umbilical cord-mesenchymal stromal cell (UC-MSC) co-culture on CB CD34+ cells and the characteristics of the resulting hiPSCs. CD34+ cells were isolated, expanded in UC-MSC co-culture for 3 days, and reprogrammed into hiPSCs using episomal vectors. Results showed that UC-MSC co-culture significantly increased CD34+ cell numbers (p < 0.0001, n = 6), with a reduced population doubling time of 25.1 ± 2.1 hours compared with the control (p < 0.0004, n = 6). The yield of CD34+ cells was substantially higher in the UC-MSC co-culture group. The hiPSCs exhibited comparable reprogramming efficiency, pluripotency marker expression, trilineage differentiation potential, and genomic stability to CD34+ cells expanded under standard culture conditions. These findings suggest that CD34+ cells from CB, expanded in UC-MSC co-culture, can be reprogrammed into functional hiPSCs without compromising cell quality or genetic stability.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"164-176"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reaching the Holy Grail: Making Hematopoietic Stem Cells in a Dish. 达到圣杯：在盘中制造造血干细胞。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-26 DOI: 10.1089/cell.2024.0085

Riccardo Piussi, Andrea Ditadi

引用次数: 0

Reprogramming Stars #19: Upgrading Cell Fate Conversions with Engineered Reprogramming Factors-An Interview with Dr. Ralf Jauch. 重编程之星 #19：利用工程重编程因子升级细胞命运转换--专访 Ralf Jauch 博士。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0094

Ralf Jauch, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

Protective Effect and Molecular Mechanism of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Diabetic Foot Ulcers.

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-11-29 DOI: 10.1089/cell.2024.0062

Jian Zhao, Yan Gu, Peng Hou

{"title":"Protective Effect and Molecular Mechanism of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Diabetic Foot Ulcers.","authors":"Jian Zhao, Yan Gu, Peng Hou","doi":"10.1089/cell.2024.0062","DOIUrl":"https://doi.org/10.1089/cell.2024.0062","url":null,"abstract":"This study explores the protective mechanism of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in diabetic foot ulcer (DFU). Human umbilical cord MSCs (HucMSCs) were identified via osteogenesis and adipogenic differentiation, as well as flow cytometry. EVs were isolated from HucMSCs and characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. Fluorescence microscopy revealed the uptake of PKH67-labeled EVs and Cy3-labeled microRNA-21-5p (miR-21-5p) by human skin fibroblasts (HSFs). EVs were cocultured with HSFs, and cell proliferation and migration were assessed using Cell Counting Kit-8, colony formation, scratch, and Transwell assays. miR-21-5p overexpression in EVs was evaluated for its role in promoting HSF functions. The expression levels of miR-21-5p, Krüppel-like factor 6 (KLF6), α-smooth muscle actin, and collagen type I alpha 1 chain were analyzed via quantitative real-time PCR and Western blotting. The interaction between miR-21-5p and KLF6 was confirmed through a dual-luciferase reporter gene assay. HucMSC-derived EVs enhanced the proliferation and migration of HSFs under high glucose by delivering miR-21-5p, which targeted and inhibited KLF6. Overexpression of KLF6 counteracted the pro-proliferative and migratory effects of EVs carrying miR-21-5p. Overall, these findings suggest that HucMSC-EVs promote HSF proliferation and migration by downregulating KLF6 via miR-21-5p delivery, offering a potential therapeutic strategy for DFU.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #18: Engineering Cell Fates and Preventing Disease by Repressing Unwanted Plasticity-An Interview with Dr. Moritz Mall. 重编程之星 #18：通过抑制不必要的可塑性来改造细胞命运和预防疾病--莫里茨-马尔博士访谈录。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-10-01 DOI: 10.1089/cell.2024.36789.mor

Moritz Mall, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

A New Frontier in Tumor Eradication: Harnessing In Vivo Cellular Reprogramming for Durable Cancer Immunotherapy. 根除肿瘤的新前沿：利用体内细胞重编程实现持久的癌症免疫疗法。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-10-01 Epub Date: 2024-10-10 DOI: 10.1089/cell.2024.0077

Constantinos Chronis

引用次数: 0

Deciphering the Sertoli Cell Signaling Pathway with Protein-Protein Interaction, Single-Cell Sequencing, and Gene Ontology. 利用蛋白质-蛋白质相互作用、单细胞测序和基因本体解密Sertoli细胞信号通路

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-10-01 DOI: 10.1089/cell.2024.0059

Danial Hashemi Karoii, Gohar Javadzadeh, Hossein Azizi, Fadhil Farhood M Al-Joborae, Mehdi Amirian

{"title":"Deciphering the Sertoli Cell Signaling Pathway with Protein-Protein Interaction, Single-Cell Sequencing, and Gene Ontology.","authors":"Danial Hashemi Karoii, Gohar Javadzadeh, Hossein Azizi, Fadhil Farhood M Al-Joborae, Mehdi Amirian","doi":"10.1089/cell.2024.0059","DOIUrl":"https://doi.org/10.1089/cell.2024.0059","url":null,"abstract":"Spermatogenesis constitutes a complex and intricate cascade of differentiation, indispensable for the male reproductive competence. The intercellular communication conduits of Sertoli cells (SCs) are pivotal in orchestrating this cascade ensuring sustenance and development of germ cells. Single cells and bioinformatics recently demonstrated articles are used for the regulatory modalities through which SCs modulate spermatogenesis, specifically via androgen receptors (ARs), the transforming growth factor-beta/Smad axis, mitogen-activated protein kinases, cAMP/protein kinase A (PKA), phosphatidylinositol 4,5-bisphosphate 3-kinase (PI3k)/AKT serine threonine kinase (Akt), AMP-activated protein kinase, and AR pathways. Within this framework, homeostasis of gap junction dynamics, cryptic sites and the activities at tight junctions and adherens junctions, with the integrity of the testicular barrier, glucose assimilation, lactate distribution, being governed also along with SC maturation. Disruptions in activities or abnormal concentration in derangements in AR, cAMP/PKA, and PI3k/Akt pathways, and as well as the molecules that comprise them, would present male infertility.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 5","pages":"135-145"},"PeriodicalIF":1.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rewinding the Tape to Identify Intrinsic Determinants of Reprogramming Potential. 倒带以确定重编程潜能的内在决定因素。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-08-01 Epub Date: 2024-08-08 DOI: 10.1089/cell.2024.0035

Kate E Galloway

引用次数: 0