Cellular reprogramming最新文献

Rejuvenation to the Heart: Overcoming Age-Related Metabolic Barriers in Direct Cardiac Reprogramming. 心脏再生：克服直接心脏重编程中与年龄相关的代谢障碍。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-15 DOI: 10.1089/cell.2025.0009

Pedro P Cunha, Mariana Lopes

引用次数: 0

Delivery Systems in Neuronal Direct Cell Reprogramming. 神经元直接细胞重编程中的传递系统。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-15 DOI: 10.1089/cell.2025.0008

Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H A M Fens, Massimiliano Caiazzo

{"title":"Delivery Systems in Neuronal Direct Cell Reprogramming.","authors":"Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H A M Fens, Massimiliano Caiazzo","doi":"10.1089/cell.2025.0008","DOIUrl":"https://doi.org/10.1089/cell.2025.0008","url":null,"abstract":"Neuronal direct cell reprogramming approach allows direct conversion of somatic cells into neurons via forced expression of neuronal cell-lineage transcription factors (TFs). These so-called induced neuronal cells have significant potential as research tools and for therapeutic applications, such as in cell replacement therapy. However, the optimization of TF delivery strategies is crucial to reach clinical practice. In this review, we outlined the currently explored delivery technologies in neuronal direct cell reprogramming and their limitations and advantages. The first employed delivery strategies were mainly integrating viral systems, such as lentiviruses that exert consistently high transgene expression in most cell types. On the other hand, viral systems cause major safety concerns, including the risk for insertional mutagenesis and inflammation. More recently, several safer nonviral delivery systems have been investigated as well; however, these systems generally exert inferior reprogramming efficiency compared with viral systems. Emerging delivery technologies could provide new opportunities in the achievement of safe and effective delivery for neuronal direct cell reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases. 磷脂酶C δ 3及其在肿瘤疾病中的意义的新见解。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-12 DOI: 10.1089/cell.2025.0004

Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang

{"title":"Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases.","authors":"Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang","doi":"10.1089/cell.2025.0004","DOIUrl":"https://doi.org/10.1089/cell.2025.0004","url":null,"abstract":"The phospholipase C (PLC) family plays a crucial role in the construction of biomembranes, cell growth, and signal transduction. PLC regulates multiple cellular processes by generating bioactive molecules such as inositol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These products propagate and regulate cellular signaling via calcium (Ca2+) mobilization and activation of protein kinase C (PKC), other kinases, and ion channels. Recently, the function of PLC delta 3 (PLCδ3) has been arousing great interests in the basic research of neoplastic diseases. It is demonstrated to affect multiple parts of tumor progression and promote glycolysis reprogramming. However, currently there are no conclusive reports regarding the mechanism of PLCδ3-mediated tumor progression and its importance as a prognostic biomarker in specific neoplastic diseases. Therefore, the present article aimed to illustrate (1) the correlation between the function of phospholipases in PLC family and tumor progression; (2) the PLCδ3-mediated tumor progression, mainly focusing on the signal transduction and regulation; and (3) its potential mechanism and vital targets involved in multiple malignancies.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Old Habits Die Hard: DNA Methylation Patterns Persist in Experimental Transdifferentiation. 旧习惯难改：DNA甲基化模式在实验转分化中持续存在。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-12 DOI: 10.1089/cell.2025.0020

Alice E Lord, Leo J Dudley, Lynette Graver, Gabriella Ficz

引用次数: 0

Menstrual Blood-Derived Mesenchymal Stem Cells Improve Endometrial Receptivity in a Mouse Model of Embryonic Implantation Dysfunction. 经血源性间充质干细胞改善胚胎着床功能障碍小鼠模型的子宫内膜容受性。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-22 DOI: 10.1089/cell.2024.0071

Chao Ma, Yue Yi, Changji Guan

{"title":"Menstrual Blood-Derived Mesenchymal Stem Cells Improve Endometrial Receptivity in a Mouse Model of Embryonic Implantation Dysfunction.","authors":"Chao Ma, Yue Yi, Changji Guan","doi":"10.1089/cell.2024.0071","DOIUrl":"https://doi.org/10.1089/cell.2024.0071","url":null,"abstract":"The decrease of endometrial receptivity leads to repeated implantation failure (RIF) during in vitro fertilization and embryo transfer. To explore the therapeutic potential of menstrual blood-derived mesenchymal stem cells (MenSCs) in addressing RIF, we established a murine model of embryonic implantation dysfunction using mifepristone. Subsequently, we administered MenSCs to these mice via tail vein injection and assessed their impact on the implantation and pregnancy rates of the affected mice. Furthermore, we conducted immunohistochemical staining on uterine tissues from these mice to examine the expression of endometrial receptivity markers, specifically vascular endothelial growth factor (VEGF)-A, HAND2, and HOXA10 following MenSCs transplantation. In parallel, we conducted in vitro studies to elucidate the molecular mechanisms of cell therapy by measuring the expression levels of VEGF-A, HAND2, and HOXA10 in endometrial stromal cells using real-time PCR and western blotting. In our mifepristone-induced mouse models, we observed a reduction in both pregnancy rates and implantation sites; however, these parameters were significantly improved after MenSCs transplantation. Similarly, the expression levels of VEGF-A, HAND2, and HOXA10 in the uterine tissues of the mifepristone group were diminished, but these levels were restored following MenSCs therapy. In vitro, after mifepristone treating, the expression of VEGF-A, HAND2, and HOXA10 decreased in endometrial stromal cells, but their expression increased after MenSCs coculture supernatant. In conclusion, these results demonstrated that MenSCs transplantation could increase endometrial receptivity by upregulating VEGF-A, HAND2, and HOXA10 expression. This study suggests MenSCs as a novel stem cell candidate in the treatment of RIF.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Proliferation Potential of Differentiated and Undifferentiated Spermatogonial Stem Cells on Diverse Feeder Layers. 已分化和未分化精原干细胞在不同饲养层上的增殖潜力。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.1089/cell.2024.0066

Kiana Sojoudi, Hossein Azizi, Maryam Solaimani

{"title":"The Proliferation Potential of Differentiated and Undifferentiated Spermatogonial Stem Cells on Diverse Feeder Layers.","authors":"Kiana Sojoudi, Hossein Azizi, Maryam Solaimani","doi":"10.1089/cell.2024.0066","DOIUrl":"10.1089/cell.2024.0066","url":null,"abstract":"Spermatogonial stem cells (SSCs) play an essential role in the transfer of genetic information through generations, making studying their cellular and molecular mechanisms critical. However, since SSCs are few in mice, directly studying them is limited, requiring specialized in vitro cultivation. Feeder layers such as mouse embryonic fibroblasts (MEFs), SNL, neonate, and adult mouse testicular stromal feeder cells (TSCs) support in vitro survival and growth. To understand the effectiveness of these feeder layers on SSC proliferation, we compared MEF, SNL, neonatal, and adult TSCs. Furthermore, we identified hub genes and potential pathways in spermatogenesis. Two populations of differentiated and undifferentiated SSCs were compared for mouse SSC colony formation and proliferation effectiveness. Additionally, Cytoscape and STRING databases were employed for protein-protein interaction networks and functional gene enrichment. The expression of three hub genes, including Dazl, Zbtb16, and Stra8, was analyzed using dynamic array chips (Fluidigm) followed by statistical analysis. Our results indicated that undifferentiated SSCs favored MEF feeders, while differentiated SSCs thrived on SNL and primary TSC feeders for long-term culture. Functional enrichment results demonstrated hub genes involvement in cell differentiation, meiosis, regulation of meiotic nuclear division, cell development, and spermatogenesis. Furthermore, mRNA expression levels of Stra8, Zbtb16, and Dazl genes show different patterns among feeder layers and SSC differentiation phases.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"75-85"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nearly a Century of Nuclear Transfer Research: Milestones, Applications, and Challenges. 近一个世纪的核转移研究：里程碑、应用和挑战。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-01 Epub Date: 2025-03-11 DOI: 10.1089/cell.2024.0089

Yiren Qin

引用次数: 0

Editing the CYP19 Gene in Goat Embryos Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer Techniques. 利用CRISPR/Cas9和体细胞核移植技术编辑山羊胚胎CYP19基因

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-01 Epub Date: 2025-03-24 DOI: 10.1089/cell.2024.0109

Ahmad Pirali, Farnoosh Jafarpour, Mehdi Hajian, Seyed Hossein Hosseini Moghaddam, Reza Moradi, Nima Tanhaie-Vash, Mohsen Rahimi Andani, Tayebeh Izadi, Hanieh Shiralian-Esfahani, Zahra Safaeinejad, Wilfried Kues, Mohammad-Hossein Nasr-Esfahani, Shahin Eghbalsaied

{"title":"Editing the CYP19 Gene in Goat Embryos Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer Techniques.","authors":"Ahmad Pirali, Farnoosh Jafarpour, Mehdi Hajian, Seyed Hossein Hosseini Moghaddam, Reza Moradi, Nima Tanhaie-Vash, Mohsen Rahimi Andani, Tayebeh Izadi, Hanieh Shiralian-Esfahani, Zahra Safaeinejad, Wilfried Kues, Mohammad-Hossein Nasr-Esfahani, Shahin Eghbalsaied","doi":"10.1089/cell.2024.0109","DOIUrl":"10.1089/cell.2024.0109","url":null,"abstract":"The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system is revolutionizing genome engineering and is expected to bring significant advancements in livestock traits, including the treatment of genetic diseases. This study focuses on CRISPR/Cas9-mediated modifications of the CYP19 gene, which encodes aromatase, an enzyme crucial for converting testosterone to estrogen and essential for steroid metabolism. Guide RNAs (gRNAs) were designed to target the CYP19 gene and cloned into the pX459 vector. The recombinant plasmid was then electrotransfected into fibroblast cells from a Lori-Bakhtiari buck, and these transfected cells were used for embryo production via somatic cell nuclear transfer (SCNT). The cloned embryos were evaluated for their progression through embryonic stages, showing no significant difference in blastocyst development between knock-out and unedited groups. The knockout efficiency was 78.4% in cells and 68.9% in goat blastocysts, demonstrating the successful depletion of CYP19. We successfully achieved a high rate of CYP19 gene-edited embryos through the combined application of cell electrotransfection and SCNT technologies, while maintaining the normal developmental rate of the embryos. These embryos can be used for transfer to generate knock-out goats, providing a foundation for further studies on CYP19's role in male fertility and production traits.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"86-93"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #21: RNA Regulatory Mechanisms That Instruct Cell Identity-An Interview with Dr. Bruno Di Stefano. 重编程之星#21：指导细胞身份的RNA调节机制-采访Bruno Di Stefano博士。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.1089/cell.2025.0014

Bruno Di Stefano, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

Sox as a Functionally Conserved Link Between Unicellular Ancestors and Human Stem Cell Control. 单细胞祖先与人类干细胞控制之间的功能保守联系。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-01 Epub Date: 2025-03-24 DOI: 10.1089/cell.2025.0011

Emma U Hammarlund

引用次数: 0