Cellular reprogramming最新文献

Precision Reprogramming-Restoring Function to Aged Cells. 精确重编程-恢复老化细胞的功能。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-09 DOI: 10.1089/cell.2025.0018

Arjun Jain, Yuuki Hosokawa, Kevin Joseph

引用次数: 0

Reprogramming of Different Cell Lineages into Functional β-Cell Substitutes. 不同细胞系重编程成功能性β-细胞替代品。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-09 DOI: 10.1089/cell.2024.0102

Anna A Dattoli, Yosip Kelemen, Xiaofeng Huang

引用次数: 0

Menstrual Blood-Derived Mesenchymal Stem Cells Improve Endometrial Receptivity in a Mouse Model of Embryonic Implantation Dysfunction. 经血源性间充质干细胞改善胚胎着床功能障碍小鼠模型的子宫内膜容受性。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-04-22 DOI: 10.1089/cell.2024.0071

Chao Ma, Yue Yi, Changji Guan

{"title":"Menstrual Blood-Derived Mesenchymal Stem Cells Improve Endometrial Receptivity in a Mouse Model of Embryonic Implantation Dysfunction.","authors":"Chao Ma, Yue Yi, Changji Guan","doi":"10.1089/cell.2024.0071","DOIUrl":"10.1089/cell.2024.0071","url":null,"abstract":"The decrease of endometrial receptivity leads to repeated implantation failure (RIF) during in vitro fertilization and embryo transfer. To explore the therapeutic potential of menstrual blood-derived mesenchymal stem cells (MenSCs) in addressing RIF, we established a murine model of embryonic implantation dysfunction using mifepristone. Subsequently, we administered MenSCs to these mice via tail vein injection and assessed their impact on the implantation and pregnancy rates of the affected mice. Furthermore, we conducted immunohistochemical staining on uterine tissues from these mice to examine the expression of endometrial receptivity markers, specifically vascular endothelial growth factor (VEGF)-A, HAND2, and HOXA10 following MenSCs transplantation. In parallel, we conducted in vitro studies to elucidate the molecular mechanisms of cell therapy by measuring the expression levels of VEGF-A, HAND2, and HOXA10 in endometrial stromal cells using real-time PCR and western blotting. In our mifepristone-induced mouse models, we observed a reduction in both pregnancy rates and implantation sites; however, these parameters were significantly improved after MenSCs transplantation. Similarly, the expression levels of VEGF-A, HAND2, and HOXA10 in the uterine tissues of the mifepristone group were diminished, but these levels were restored following MenSCs therapy. In vitro, after mifepristone treating, the expression of VEGF-A, HAND2, and HOXA10 decreased in endometrial stromal cells, but their expression increased after MenSCs coculture supernatant. In conclusion, these results demonstrated that MenSCs transplantation could increase endometrial receptivity by upregulating VEGF-A, HAND2, and HOXA10 expression. This study suggests MenSCs as a novel stem cell candidate in the treatment of RIF.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"124-133"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Locking the Fate: How PROX1 Represses Plasticity and Liver Cancer. 锁定命运：PROX1如何抑制可塑性和肝癌。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-03-26 DOI: 10.1089/cell.2025.0013

Seung-Won Lee, Jungsun Kim

引用次数: 0

Rejuvenation to the Heart: Overcoming Age-Related Metabolic Barriers in Direct Cardiac Reprogramming. 心脏再生：克服直接心脏重编程中与年龄相关的代谢障碍。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1089/cell.2025.0009

Pedro P Cunha, Mariana Lopes

引用次数: 0

Reprogramming Stars #22: Cellular Reprogramming as a Route for Decoding Neurodegenerative Disorders-An Interview with Dr. Janelle Drouin-Ouellet. 重编程之星#22：细胞重编程作为解码神经退行性疾病的途径——采访Janelle Drouin-Ouellet博士。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-05-20 DOI: 10.1089/cell.2025.77701.int

Janelle Drouin-Ouellet, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

Delivery Systems in Neuronal Direct Cell Reprogramming. 神经元直接细胞重编程中的传递系统。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1089/cell.2025.0008

Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H A M Fens, Massimiliano Caiazzo

{"title":"Delivery Systems in Neuronal Direct Cell Reprogramming.","authors":"Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H A M Fens, Massimiliano Caiazzo","doi":"10.1089/cell.2025.0008","DOIUrl":"10.1089/cell.2025.0008","url":null,"abstract":"Neuronal direct cell reprogramming approach allows direct conversion of somatic cells into neurons via forced expression of neuronal cell-lineage transcription factors (TFs). These so-called induced neuronal cells have significant potential as research tools and for therapeutic applications, such as in cell replacement therapy. However, the optimization of TF delivery strategies is crucial to reach clinical practice. In this review, we outlined the currently explored delivery technologies in neuronal direct cell reprogramming and their limitations and advantages. The first employed delivery strategies were mainly integrating viral systems, such as lentiviruses that exert consistently high transgene expression in most cell types. On the other hand, viral systems cause major safety concerns, including the risk for insertional mutagenesis and inflammation. More recently, several safer nonviral delivery systems have been investigated as well; however, these systems generally exert inferior reprogramming efficiency compared with viral systems. Emerging delivery technologies could provide new opportunities in the achievement of safe and effective delivery for neuronal direct cell reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"109-123"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases. 磷脂酶C δ 3及其在肿瘤疾病中的意义的新见解。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-12 DOI: 10.1089/cell.2025.0004

Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang

{"title":"Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases.","authors":"Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang","doi":"10.1089/cell.2025.0004","DOIUrl":"https://doi.org/10.1089/cell.2025.0004","url":null,"abstract":"The phospholipase C (PLC) family plays a crucial role in the construction of biomembranes, cell growth, and signal transduction. PLC regulates multiple cellular processes by generating bioactive molecules such as inositol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These products propagate and regulate cellular signaling via calcium (Ca2+) mobilization and activation of protein kinase C (PKC), other kinases, and ion channels. Recently, the function of PLC delta 3 (PLCδ3) has been arousing great interests in the basic research of neoplastic diseases. It is demonstrated to affect multiple parts of tumor progression and promote glycolysis reprogramming. However, currently there are no conclusive reports regarding the mechanism of PLCδ3-mediated tumor progression and its importance as a prognostic biomarker in specific neoplastic diseases. Therefore, the present article aimed to illustrate (1) the correlation between the function of phospholipases in PLC family and tumor progression; (2) the PLCδ3-mediated tumor progression, mainly focusing on the signal transduction and regulation; and (3) its potential mechanism and vital targets involved in multiple malignancies.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Old Habits Die Hard: DNA Methylation Patterns Persist in Experimental Transdifferentiation. 旧习惯难改：DNA甲基化模式在实验转分化中持续存在。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-05-12 DOI: 10.1089/cell.2025.0020

Alice E Lord, Leo J Dudley, Lynette Graver, Gabriella Ficz

引用次数: 0

The Proliferation Potential of Differentiated and Undifferentiated Spermatogonial Stem Cells on Diverse Feeder Layers. 已分化和未分化精原干细胞在不同饲养层上的增殖潜力。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-04-01 Epub Date: 2025-03-20 DOI: 10.1089/cell.2024.0066

Kiana Sojoudi, Hossein Azizi, Maryam Solaimani

{"title":"The Proliferation Potential of Differentiated and Undifferentiated Spermatogonial Stem Cells on Diverse Feeder Layers.","authors":"Kiana Sojoudi, Hossein Azizi, Maryam Solaimani","doi":"10.1089/cell.2024.0066","DOIUrl":"10.1089/cell.2024.0066","url":null,"abstract":"Spermatogonial stem cells (SSCs) play an essential role in the transfer of genetic information through generations, making studying their cellular and molecular mechanisms critical. However, since SSCs are few in mice, directly studying them is limited, requiring specialized in vitro cultivation. Feeder layers such as mouse embryonic fibroblasts (MEFs), SNL, neonate, and adult mouse testicular stromal feeder cells (TSCs) support in vitro survival and growth. To understand the effectiveness of these feeder layers on SSC proliferation, we compared MEF, SNL, neonatal, and adult TSCs. Furthermore, we identified hub genes and potential pathways in spermatogenesis. Two populations of differentiated and undifferentiated SSCs were compared for mouse SSC colony formation and proliferation effectiveness. Additionally, Cytoscape and STRING databases were employed for protein-protein interaction networks and functional gene enrichment. The expression of three hub genes, including Dazl, Zbtb16, and Stra8, was analyzed using dynamic array chips (Fluidigm) followed by statistical analysis. Our results indicated that undifferentiated SSCs favored MEF feeders, while differentiated SSCs thrived on SNL and primary TSC feeders for long-term culture. Functional enrichment results demonstrated hub genes involvement in cell differentiation, meiosis, regulation of meiotic nuclear division, cell development, and spermatogenesis. Furthermore, mRNA expression levels of Stra8, Zbtb16, and Dazl genes show different patterns among feeder layers and SSC differentiation phases.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"75-85"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0