Cellular reprogramming最新文献_第2页

Cellular Reprogramming and Microfluidics Enable Large-Scale Blastoid Production from Aged Donors. 细胞重编程和微流体技术使老年捐赠者的囊胚大规模生成成为可能。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-12-01 Epub Date: 2025-10-22 DOI: 10.1177/21524971251391914

Marcelo Tigre Moura

引用次数: 0

Induction of Neuronal Differentiation of Human Dermal Mesenchymal Stem Cells by a Defined Arginine-Aspartate-Phenylalanine Peptide Mixture in Vitro. 精氨酸-天冬氨酸-苯丙氨酸混合肽体外诱导人真皮间充质干细胞神经元分化的研究。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-12-01 DOI: 10.1177/21524971251398954

Xiaoru Pan, Shengxiu Liu, Rong Zhang, Fengming Yao, Huiling Jin, Ruzhi Zhang

{"title":"Induction of Neuronal Differentiation of Human Dermal Mesenchymal Stem Cells by a Defined Arginine-Aspartate-Phenylalanine Peptide Mixture in Vitro.","authors":"Xiaoru Pan, Shengxiu Liu, Rong Zhang, Fengming Yao, Huiling Jin, Ruzhi Zhang","doi":"10.1177/21524971251398954","DOIUrl":"https://doi.org/10.1177/21524971251398954","url":null,"abstract":"The aim of this study is to investigate the effects of a peptide mixture composed of arginine (Arg), aspartic acid (Asp), and phenylalanine (Phe) on the proliferation and neural differentiation of human dermal mesenchymal stem cells (HDMSCs). HDMSCs were isolated from residual skin tissue following circumcision and purified using a combination of long-term trypsin stress and suspension culture. The impact of the peptide mixture on HDMSC proliferation was assessed at various concentrations using a Cell Counting Kit-8 (CCK-8) assay. Based on preliminary screening of proliferative activity, an optimal peptide mixture concentration for promoting proliferation was selected for further HDMSCs culture. During this culture, morphological changes were observed. Differentiation efficiency was evaluated using immunofluorescence staining for the neural markers Nestin and Neurofilament-L (NF-L) at weeks 3 and 6 of culture, respectively. Primary adherent HDMSCs exhibited a long spindle morphology and formed spherical cell clusters in suspension culture. A peptide mixture at concentrations of 5 ng/mL Arg, 20 ng/mL Asp, and 40 ng/mL Phe significantly promoted HDMSC proliferation (p < 0.05). During the induction period, the cells gradually acquired neuronal morphological characteristics, including cell body contraction and process extension. Immunofluorescence results showed that the cells expressed Nestin by week 3, shifting to NF-L positivity by week 6. This indicates that the peptide mixture induced the differentiation of HDMSCs into neural cells. A peptide mixture containing Arg, Asp, and Phe has been shown to effectively induce the differentiation of HDMSCs into neural cells. This provides a novel and safe strategy for the regeneration of neural tissue.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"27 6","pages":"228-236"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145630543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Application of Cell Therapy in the Treatment of Female Reproductive Diseases: A Systematic Review. 细胞疗法在女性生殖疾病治疗中的临床应用综述

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-10-01 Epub Date: 2025-09-23 DOI: 10.1177/21524971251379699

Fatemeh Saberi, Zeinab Dehghan, Shayesteh Mehdinejadiani, Zahra Khosravizadeh, Effat Noori, Tayyebeh Pilehchi, Delsuz Rezaee, Zahra Taheri, Azam Govahi, Nasim Goudarzi, Kobra Mehdinejadiani, Forough Shams

{"title":"Clinical Application of Cell Therapy in the Treatment of Female Reproductive Diseases: A Systematic Review.","authors":"Fatemeh Saberi, Zeinab Dehghan, Shayesteh Mehdinejadiani, Zahra Khosravizadeh, Effat Noori, Tayyebeh Pilehchi, Delsuz Rezaee, Zahra Taheri, Azam Govahi, Nasim Goudarzi, Kobra Mehdinejadiani, Forough Shams","doi":"10.1177/21524971251379699","DOIUrl":"10.1177/21524971251379699","url":null,"abstract":"Reproductive disorders affect millions of women worldwide, playing a crucial role in determining female fertility health and quality of life. Conventional methods such as surgery, hormone therapy, and assisted reproductive technologies can be successful in some cases, but are limited by adverse effects, and limited effectiveness. In recent years, cell therapy has provided new possibilities for treating various infertility disorders. The articles extracted from PubMed and Scopus databases were based on cell therapy premature ovarian failure (POF), intrauterine adhesions, Asherman syndrome (AS), recurrent implantation failure (RIF), repeat implantation failure, polycystic ovary syndrome (PCOS), endometriosis, preeclampsia, and clinical trials. The collected articles were added to EndNote X7, and review articles along with duplicate studies were eliminated. Several studies have indicated that peripheral blood mononuclear cells, autologous platelet-rich plasma, mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), adipose-derived stromal vascular fraction, and umbilical cord stem cells can be used to treat reproductive diseases, including POF, AS, and RIF. PCOS, endometriosis, and preeclampsia were deleted from the study, because there were no clinical cell therapy studies for these diseases. Among the 210 studies, 28 were selected as eligible for further evaluation. Various clinical trials have supported the role of cell therapy in treating reproductive disorders. Although the information from this systematic review is promising, further studies are needed to evaluate the efficacy and safety of these and other cells in treating infertility.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"184-198"},"PeriodicalIF":1.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Reprogramming-Restoring Function to Aged Cells. 精确重编程-恢复老化细胞的功能。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-10-01 Epub Date: 2025-06-09 DOI: 10.1089/cell.2025.0018

Arjun Jain, Yuuki Hosokawa, Kevin Joseph

引用次数: 0

Reprogramming Stars #24: Pluripotency and Oncogenic Transformation: A Series of Intermingling Events-An Interview with Dr. Fabrice Lavial. 重编程之星#24：多能性和致癌转化：一系列相互交织的事件——法布里斯·拉维尔博士访谈

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-10-01 Epub Date: 2025-09-25 DOI: 10.1177/21524971251378893

Fabrice Lavial, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

In Vitro Hepatocyte Differentiation of Human Adipose-Derived Stem Cells Under Hypoxia and Photobiomodulation Irradiation. 缺氧和光生物调节照射下人脂肪干细胞体外肝细胞分化的研究。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-10-01 Epub Date: 2025-07-28 DOI: 10.1177/21524971251363134

In-Su Park

{"title":"In Vitro Hepatocyte Differentiation of Human Adipose-Derived Stem Cells Under Hypoxia and Photobiomodulation Irradiation.","authors":"In-Su Park","doi":"10.1177/21524971251363134","DOIUrl":"10.1177/21524971251363134","url":null,"abstract":"Stem cells may be manipulated in vitro to induce hepatic differentiation. We investigated the effect of hypoxia and photobiomodulation therapy (PBMT) on the hepatogenic differentiation of human adipose-derived stem cells (hASCs). hASCs were exposed to different carbon dioxide concentrations with photobiomodulation (PBM) using low-level light. Cell survival and secretion of hepatocyte growth factor (HGF) of the hASCs were evaluated by immunostaining and Western blot analyses. Hepatic differentiation was assessed via immunocytochemical staining, fluorescence-activated cell sorting, and Western blot analysis for liver-specific genes and proteins, including albumin (ALB), cytokeratins 8/18, and alpha-fetoprotein (AFP). PBM therapy has been shown to enhance proliferation and cytokine secretion of a number of cells. The expression profiles of ALB, AFP, and cytokeratin 8/18 demonstrated that when HGF, hypoxia, or PBMT were treated individually, incomplete hepatocyte differentiation was achieved. In contrast, quantitative analysis of ALB, cytokeratins 8/18, and AFP showed that HGF was enhanced significantly by hypoxia+PBM treatment. The obtained cell populations contained progenitors that expressed both hepatic ALB and cytokeratin 8/18 markers, as well as AFP. These data suggest that PBMT and hypoxia are effective biostimulators of hASCs in hepatogenic differentiation, which enhances the survival of hASCs and stimulates the secretion of growth factors.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"199-204"},"PeriodicalIF":1.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #23: Charting Cell Fate Crossroads from the Interplay Between Epigenetics, Transcription, and 3D Chromatin Architecture-An Interview with Dr. Effie Apostolou. 重编程之星#23：从表观遗传学，转录和3D染色质结构之间的相互作用绘制细胞命运十字路口-对Effie Apostolou博士的采访。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-08-01 Epub Date: 2025-08-05 DOI: 10.1177/21524971251366924

Effie Apostolou, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases. 磷脂酶C δ 3及其在肿瘤疾病中的意义的新见解。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-08-01 Epub Date: 2025-05-12 DOI: 10.1089/cell.2025.0004

Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang

{"title":"Novel Insights into the Phospholipase C Delta 3 and Its Implications in Neoplastic Diseases.","authors":"Lindi Xu, Zhenli Li, Shuaishuai Zhu, Xingshun Qi, Wei Zhang, Yufu Tang","doi":"10.1089/cell.2025.0004","DOIUrl":"10.1089/cell.2025.0004","url":null,"abstract":"The phospholipase C (PLC) family plays a crucial role in the construction of biomembranes, cell growth, and signal transduction. PLC regulates multiple cellular processes by generating bioactive molecules such as inositol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These products propagate and regulate cellular signaling via calcium (Ca2+) mobilization and activation of protein kinase C (PKC), other kinases, and ion channels. Recently, the function of PLC delta 3 (PLCδ3) has been arousing great interests in the basic research of neoplastic diseases. It is demonstrated to affect multiple parts of tumor progression and promote glycolysis reprogramming. However, currently there are no conclusive reports regarding the mechanism of PLCδ3-mediated tumor progression and its importance as a prognostic biomarker in specific neoplastic diseases. Therefore, the present article aimed to illustrate (1) the correlation between the function of phospholipases in PLC family and tumor progression; (2) the PLCδ3-mediated tumor progression, mainly focusing on the signal transduction and regulation; and (3) its potential mechanism and vital targets involved in multiple malignancies.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"146-153"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformation of an Olfactory Placode-Derived Cell into One with Stem Cell Characteristics by Disrupting Epigenetic Barriers. 通过破坏表观遗传屏障将嗅觉基板衍生细胞转化为具有干细胞特征的细胞。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-08-01 Epub Date: 2025-07-14 DOI: 10.1177/21524971251359000

Ghazia Abbas, Rutesh Vyas, Joyce C Noble, Brian Lin, Robert P Lane

{"title":"Transformation of an Olfactory Placode-Derived Cell into One with Stem Cell Characteristics by Disrupting Epigenetic Barriers.","authors":"Ghazia Abbas, Rutesh Vyas, Joyce C Noble, Brian Lin, Robert P Lane","doi":"10.1177/21524971251359000","DOIUrl":"10.1177/21524971251359000","url":null,"abstract":"The mammalian olfactory neuronal lineage is regenerative, and accordingly, maintains a population of pluripotent cells that replenish olfactory sensory neurons and other olfactory cell types during the life of the animal. Moreover, in response to acute injury, the early transit amplifying cells along the olfactory sensory neuronal lineage are able to de-differentiate to shift resources in support of tissue restoration. In order to further explore plasticity of various cellular stages along the olfactory sensory neuronal lineage, we challenged the epigenetic stability of olfactory placode-derived cell lines that model immature olfactory sensory neuronal stages. We found that perturbation of the Ehmt2 chromatin modifier transformed the growth properties, morphology, and gene expression profiles toward states with several stem cell characteristics. This transformation was dependent on continued expression of the large T-antigen, and was enhanced by Sox2 over-expression. These findings may provide momentum for exploring inherent cellular plasticity within early cell types of the olfactory lineage, as well as potentially add to our knowledge of cellular reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"164-173"},"PeriodicalIF":1.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Old Habits Die Hard: DNA Methylation Patterns Persist in Experimental Transdifferentiation. 旧习惯难改：DNA甲基化模式在实验转分化中持续存在。

IF 1.7 4区医学

Cellular reprogramming Pub Date : 2025-08-01 Epub Date: 2025-05-12 DOI: 10.1089/cell.2025.0020

Alice E Lord, Leo J Dudley, Lynette Graver, Gabriella Ficz

引用次数: 0