Cellular reprogramming最新文献_第2页

Locking the Fate: How PROX1 Represses Plasticity and Liver Cancer. 锁定命运：PROX1如何抑制可塑性和肝癌。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-03-26 DOI: 10.1089/cell.2025.0013

Seung-Won Lee, Jungsun Kim

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Reprogramming Stars #20: Attenuating Cancer Cell Memory and Discovering Cancer Biomarkers with Cellular Reprogramming-An Interview with Dr. Jungsun Kim. 重编程之星#20：用细胞重编程减弱癌细胞记忆和发现癌症生物标志物——采访jung - sun Kim博士

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-02-01 Epub Date: 2025-01-29 DOI: 10.1089/cell.2025.0007

Jungsun Kim, Mariana Lopes, Carlos-Filipe Pereira

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Bidirectional Prime Editing: Combining Precision with Versatility for Genome Editing. 双向启动编辑：基因组编辑的精确性与多功能性相结合。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-02-01 Epub Date: 2024-12-17 DOI: 10.1089/cell.2024.0075

Mahmood S Choudhery, Taqdees Arif, Ruhma Mahmood

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Molecular Mechanisms and Strategies for Inducing Neuronal Differentiation in Glioblastoma Cells. 胶质母细胞瘤细胞诱导神经元分化的分子机制和策略。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-02-01 Epub Date: 2025-01-30 DOI: 10.1089/cell.2024.0087

Zhao-Qi Tang, Yan-Rong Ye, Yun Shen

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Acknowledgment of Reviewers 2024. 审稿人致谢

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-02-01 DOI: 10.1089/cell.2024.10086.revack

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Protective Effect and Molecular Mechanism of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Diabetic Foot Ulcers. 间充质干细胞来源的细胞外囊泡对糖尿病足溃疡的保护作用及其分子机制。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-02-01 Epub Date: 2024-11-29 DOI: 10.1089/cell.2024.0062

Jian Zhao, Yan Gu, Peng Hou

{"title":"Protective Effect and Molecular Mechanism of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Diabetic Foot Ulcers.","authors":"Jian Zhao, Yan Gu, Peng Hou","doi":"10.1089/cell.2024.0062","DOIUrl":"10.1089/cell.2024.0062","url":null,"abstract":"This study explores the protective mechanism of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in diabetic foot ulcer (DFU). Human umbilical cord MSCs (HucMSCs) were identified via osteogenesis and adipogenic differentiation, as well as flow cytometry. EVs were isolated from HucMSCs and characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. Fluorescence microscopy revealed the uptake of PKH67-labeled EVs and Cy3-labeled microRNA-21-5p (miR-21-5p) by human skin fibroblasts (HSFs). EVs were cocultured with HSFs, and cell proliferation and migration were assessed using Cell Counting Kit-8, colony formation, scratch, and Transwell assays. miR-21-5p overexpression in EVs was evaluated for its role in promoting HSF functions. The expression levels of miR-21-5p, Krüppel-like factor 6 (KLF6), α-smooth muscle actin, and collagen type I alpha 1 chain were analyzed via quantitative real-time PCR and Western blotting. The interaction between miR-21-5p and KLF6 was confirmed through a dual-luciferase reporter gene assay. HucMSC-derived EVs enhanced the proliferation and migration of HSFs under high glucose by delivering miR-21-5p, which targeted and inhibited KLF6. Overexpression of KLF6 counteracted the pro-proliferative and migratory effects of EVs carrying miR-21-5p. Overall, these findings suggest that HucMSC-EVs promote HSF proliferation and migration by downregulating KLF6 via miR-21-5p delivery, offering a potential therapeutic strategy for DFU.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"33-44"},"PeriodicalIF":1.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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RNA in Stow, Leukemia on the Go: P-Bodies RNA Sequestration Drives Leukemogenesis. 在Stow，白血病的RNA: p -体RNA隔离驱动白血病发生。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2025-02-01 Epub Date: 2025-02-03 DOI: 10.1089/cell.2024.0092

Alexandre Germanos, Sowndarya Muthukumar, Cristian Bellodi

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Reprogramming Stars #17: Breaking Down the Barriers of Direct Reprogramming Using a Model Organism-An Interview with Dr. Baris Tursun. 重编程之星#17：使用模型生物打破直接重编程的障碍-对Baris Tursun博士的采访。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-26 DOI: 10.1089/cell.2024.54625

Baris Tursun, Mariana Lopes, Carlos-Filipe Pereira

引用次数: 0

Transplantation of Human Induced Pluripotent Stem Cell-Derived Airway Epithelia at Different Induction Stages into Nude Rat. 将不同诱导阶段的人类诱导多能干细胞衍生气道上皮细胞移植到裸鼠体内

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0054

Keisuke Mizuno, Hiroe Ohnishi, Yo Kishimoto, Hideaki Okuyama, Yoshitaka Kawai, Masayuki Kitano, Yasuyuki Hayashi, Koichi Omori

{"title":"Transplantation of Human Induced Pluripotent Stem Cell-Derived Airway Epithelia at Different Induction Stages into Nude Rat.","authors":"Keisuke Mizuno, Hiroe Ohnishi, Yo Kishimoto, Hideaki Okuyama, Yoshitaka Kawai, Masayuki Kitano, Yasuyuki Hayashi, Koichi Omori","doi":"10.1089/cell.2024.0054","DOIUrl":"10.1089/cell.2024.0054","url":null,"abstract":"Tracheal reconstruction is necessary in patients with large tracheal defects. Previously, artificial tracheae made of polypropylene and collagen sponge have been used clinically by our group. As a basic research aimed at promoting epithelialization for infection defense, we transplanted cell sheets of human induced pluripotent stem cell (hiPSC)-derived airway epithelial cells (iAECs) with artificial tracheae into tracheal defects of rats and confirmed their engraftment. In this study, we examined the difference in the cell engraftment between hiPSC-derived airway epithelial progenitor cells (iAEPCs) and iAECs. Cell sheets were collected on days 38, 45, and 56 of induction into iAECs, then transplanted into nude rats with tracheal defects along with the artificial trachea. Two weeks after transplantation, surviving human nuclear antigen (HNA)-positive epithelial cells were observed none of six rats in the 38-day group, two out of six in 45-day group, and five out of six in the 56-day group. The proportion of surviving HNA+ cells among the epithelial cells of 56-day group was significantly higher those of 38-day group. Differentiated iAECs are more suitable for the transplantation of hiPSCs into tracheal defects. Our findings propose the use of differentiated cells for improvement of engraftment efficiency.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"156-163"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming of Expanded Cord Blood-Derived CD34⁺ Cells from Umbilical Cord-Mesenchymal Stromal Cell Co-Culture to Generate Human-Induced Pluripotent Stem Cells. 从脐带-间质基质细胞共培养中对扩增的脐带血CD34+细胞进行重编程，以生成人类诱导多能干细胞。

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0073

Fatin Fazrina Roslan, Yuexin Yu, Mengmeng Wang, Nurul Ain Nasim Mohd Yusof, Ghee Chien Ooi, Khong Lek Then, Kong Yong Then, Soon-Keng Cheong, Mohd Nor Azim Ab Patar, Jun Jie Tan

{"title":"Reprogramming of Expanded Cord Blood-Derived CD34+ Cells from Umbilical Cord-Mesenchymal Stromal Cell Co-Culture to Generate Human-Induced Pluripotent Stem Cells.","authors":"Fatin Fazrina Roslan, Yuexin Yu, Mengmeng Wang, Nurul Ain Nasim Mohd Yusof, Ghee Chien Ooi, Khong Lek Then, Kong Yong Then, Soon-Keng Cheong, Mohd Nor Azim Ab Patar, Jun Jie Tan","doi":"10.1089/cell.2024.0073","DOIUrl":"10.1089/cell.2024.0073","url":null,"abstract":"Cord blood (CB) is widely stored as a source of hematopoietic stem cells for potential future use, though its application for autologous purposes remains limited. Repurposing CB into human-induced pluripotent stem cells (hiPSCs) can broaden its utility beyond hematological conditions. This study investigated the effects of umbilical cord-mesenchymal stromal cell (UC-MSC) co-culture on CB CD34+ cells and the characteristics of the resulting hiPSCs. CD34+ cells were isolated, expanded in UC-MSC co-culture for 3 days, and reprogrammed into hiPSCs using episomal vectors. Results showed that UC-MSC co-culture significantly increased CD34+ cell numbers (p < 0.0001, n = 6), with a reduced population doubling time of 25.1 ± 2.1 hours compared with the control (p < 0.0004, n = 6). The yield of CD34+ cells was substantially higher in the UC-MSC co-culture group. The hiPSCs exhibited comparable reprogramming efficiency, pluripotency marker expression, trilineage differentiation potential, and genomic stability to CD34+ cells expanded under standard culture conditions. These findings suggest that CD34+ cells from CB, expanded in UC-MSC co-culture, can be reprogrammed into functional hiPSCs without compromising cell quality or genetic stability.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"164-176"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0