Cellular reprogramming最新文献_第3页

Partial Reprogramming as a Method for Regenerating Neural Tissues in Aged Organisms. 部分重编程作为老化生物体神经组织再生的一种方法

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2024-02-01 DOI: 10.1089/cell.2023.0123

Ali Saber Sichani, Somayeh Khoddam, Shayan Shakeri, Zahra Tavakkoli, Arad Ranji Jafroodi, Reza Dabbaghipour, Mohsen Sisakht, Jafar Fallahi

{"title":"Partial Reprogramming as a Method for Regenerating Neural Tissues in Aged Organisms.","authors":"Ali Saber Sichani, Somayeh Khoddam, Shayan Shakeri, Zahra Tavakkoli, Arad Ranji Jafroodi, Reza Dabbaghipour, Mohsen Sisakht, Jafar Fallahi","doi":"10.1089/cell.2023.0123","DOIUrl":"10.1089/cell.2023.0123","url":null,"abstract":"Aging causes numerous age-related diseases, leading the human species to death. Nevertheless, rejuvenating strategies based on cell epigenetic modifications are a possible approach to counteract disease progression while getting old. Cell reprogramming of adult somatic cells toward pluripotency ought to be a promising tool for age-related diseases. However, researchers do not have control over this process as cells lose their fate, and cause potential cancerous cells or unexpected cell phenotypes. Direct and partial reprogramming were introduced in recent years with distinctive applications. Although direct reprogramming makes cells lose their identity, it has various applications in regeneration medicine. Temporary and regulated in vivo overexpression of Yamanaka factors has been shown in several experimental contexts to be achievable and is used to rejuvenate mice models. This regeneration can be accomplished by altering the epigenetic adult cell signature to the signature of a younger cell. The greatest advantage of partial reprogramming is that this method does not allow cells to lose their identity when they are resetting their epigenetic clock. It is a regimen of short-term Oct3/4, Sox2, Klf4, and c-Myc expression in vivo that prevents full reprogramming to the pluripotent state and avoids both tumorigenesis and the presence of unwanted undifferentiated cells. We know that many neurological age-related diseases, such as Alzheimer's disease, stroke, dementia, and Parkinson's disease, are the main cause of death in the last decades of life. Therefore, scientists have a special tendency regarding neuroregeneration methods to increase human life expectancy.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 1","pages":"10-23"},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Therapy-Mediated Partial Reprogramming Extends Lifespan and Reverses Age-Related Changes in Aged Mice. 基因疗法介导的部分重编程延长了老年小鼠的寿命并逆转了与年龄有关的变化。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2024-02-01 DOI: 10.1089/cell.2023.0072

Carolina Cano Macip, Rokib Hasan, Victoria Hoznek, Jihyun Kim, Yuancheng Ryan Lu, Louis E Metzger, Saumil Sethna, Noah Davidsohn

{"title":"Gene Therapy-Mediated Partial Reprogramming Extends Lifespan and Reverses Age-Related Changes in Aged Mice.","authors":"Carolina Cano Macip, Rokib Hasan, Victoria Hoznek, Jihyun Kim, Yuancheng Ryan Lu, Louis E Metzger, Saumil Sethna, Noah Davidsohn","doi":"10.1089/cell.2023.0072","DOIUrl":"10.1089/cell.2023.0072","url":null,"abstract":"Aging is a complex progression of changes best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ function. Although aging cannot currently be prevented, its impact on life- and healthspan in the elderly can potentially be minimized by interventions that aim to return these cellular processes to optimal function. Recent studies have demonstrated that partial reprogramming using the Yamanaka factors (or a subset; OCT4, SOX2, and KLF4; OSK) can reverse age-related changes in vitro and in vivo. However, it is still unknown whether the Yamanaka factors (or a subset) are capable of extending the lifespan of aged wild-type (WT) mice. In this study, we show that systemically delivered adeno-associated viruses, encoding an inducible OSK system, in 124-week-old male mice extend the median remaining lifespan by 109% over WT controls and enhance several health parameters. Importantly, we observed a significant improvement in frailty scores indicating that we were able to improve the healthspan along with increasing the lifespan. Furthermore, in human keratinocytes expressing exogenous OSK, we observed significant epigenetic markers of age reversal, suggesting a potential reregulation of genetic networks to a younger potentially healthier state. Together, these results may have important implications for the development of partial reprogramming interventions to reverse age-associated diseases in the elderly.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 1","pages":"24-32"},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10909732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #14: Fast-Forwarding Cellular Reprogramming- An Interview with Dr. Mark Kotter. 重编程之星 #14：快速推进细胞重编程--马克-科特博士访谈。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2024-02-01 DOI: 10.1089/cell.2024.29111.mk

Mark R Kotter, Carlos-Filipe Pereira

引用次数: 0

Cloned Foal Born from Postmortem-Obtained Ear Sample Refrigerated for 5 Days Before Fibroblast Isolation and Decontamination of the Infected Monolayer Culture. 在成纤维细胞分离和净化受感染的单层培养物之前，将从死后获得的耳部样本冷藏 5 天，克隆出小马。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2024-02-01 Epub Date: 2024-01-23 DOI: 10.1089/cell.2023.0076

Jenin V Cortez, Kylie Hardwicke, Christopher G Grupen, Muren Herrid, Zoltan Machaty, Gábor Vajta

{"title":"Cloned Foal Born from Postmortem-Obtained Ear Sample Refrigerated for 5 Days Before Fibroblast Isolation and Decontamination of the Infected Monolayer Culture.","authors":"Jenin V Cortez, Kylie Hardwicke, Christopher G Grupen, Muren Herrid, Zoltan Machaty, Gábor Vajta","doi":"10.1089/cell.2023.0076","DOIUrl":"10.1089/cell.2023.0076","url":null,"abstract":"A 6-year-old mare, a valuable polo horse, died of complications following postcolic surgery. To preserve its genetics, ear skin samples were collected immediately after death and stored in an equine embryo transfer medium at 4°C for 5 days. After trypsin digestion, monolayer fibroblast cultures were established, but signs of massive bacterial infection were found in all of them. As an ultimate attempt for rescue, rigorously and repeatedly washed cells were individually cultured in all wells of four 96-well dishes. New monolayers were established from the few wells without contamination and used for somatic cell nuclear transfer. Four of the six Day 7 blastocysts derived from 14 reconstructed zygotes were transferred in four naturally cycling mares on Day 5 after ovulation. The embryo transfers resulted in 2 pregnancies, one from a fresh and one from a vitrified blastocyst. The vitrified embryo transfer resulted in a healthy offspring, now 21 months old, genetically and phenotypically identical to the somatic cell donor animal.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"33-36"},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2023. 鸣谢 2023 年审稿人。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-22 DOI: 10.1089/cell.2024.29105.ack

引用次数: 0

Sustained Vision Recovery by OSK Gene Therapy in a Mouse Model of Glaucoma 在青光眼小鼠模型中通过 OSK 基因疗法持续恢复视力

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-07 DOI: 10.1089/cell.2023.0074

M. Karg, Y. Lu, Nasrin Refaian, James Cameron, Emma Hoffmann, Cindy Hoppe, Shintaro Shirahama, Madhura Shah, Drenushe Krasniqi, A. Krishnan, Maleeka Shrestha, Yinjie Guo, Jennifer M. Cermak, Michel Walthier, Kasia Broniowska, Sharon Rosenzweig-Lipson, M. Gregory-Ksander, David A. Sinclair, Bruce R. Ksander

引用次数: 0

SIRT6 Knockdown in Buffalo Fetal Fibroblasts Exacerbates Premature Senescence Caused by DNA and Telomere Damage. 水牛胎儿成纤维细胞SIRT6基因敲除加剧DNA和端粒损伤引起的早衰。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 Epub Date: 2023-09-19 DOI: 10.1089/cell.2023.0048

Jingyuan Liang, Jiayu Cui, Juanru Cheng, Yu Pan, Ruimen Zhang, Sufang Yang, Lingxiu Zou

{"title":"SIRT6 Knockdown in Buffalo Fetal Fibroblasts Exacerbates Premature Senescence Caused by DNA and Telomere Damage.","authors":"Jingyuan Liang, Jiayu Cui, Juanru Cheng, Yu Pan, Ruimen Zhang, Sufang Yang, Lingxiu Zou","doi":"10.1089/cell.2023.0048","DOIUrl":"10.1089/cell.2023.0048","url":null,"abstract":"As a gene with antiaging functions, sirtuin6 (SIRT6) belonging to the sirtuin family plays a vital role in DNA repair, telomerase function, and cellular senescence, as well as maintains epigenomic stability and promotes longevity. However, its role in cell senescence in large animals, such as buffaloes, remains unknown. Fibroblasts are commonly used for somatic reprogramming, and their physiological characteristics affect the efficiency of this process. We aimed to elucidate the role of SIRT6 in cellular senescence and proliferation and analyze its effect on the biological function of buffalo fibroblasts to help improve the efficiency of buffalo somatic cell reprogramming. The expression of SIRT6 and related DNA damage was measured in buffalo fibroblasts obtained at different developmental stages (in the fetus and at 3 and 10 years of age), and the effect of SIRT6 knockdown on the senescence of buffalo fetal fibroblast was investigated. An inverse relationship was observed between SIRT6 expression and senescence in buffalo fibroblasts obtained from animals of various ages. This was accompanied by decreased cell growth, viability, and increased DNA damage. Short hairpin RNA-mediated SIRT6 knockdown accelerated the senescence of buffalo fetal fibroblasts. It blocked the cell cycle during in vitro cell culture, which further enhanced DNA damage, particularly with respect to the telomeres. Collectively, our findings suggest that SIRT6 expression was closely associated with buffalo senescence in fibroblasts. These findings serve as a foundation to better understand the cellular functions of SIRT6 and also aid in selecting donor cells for buffalo somatic cell reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"277-287"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41112255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allogeneic Mesenchymal Stem Cells After In Vivo Transplantation: A Review. 同种异体间充质干细胞体内移植研究进展

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 Epub Date: 2023-11-16 DOI: 10.1089/cell.2023.0084

Derek B Asserson

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Low-Intensity Nanosecond Pulsed Electric Field Accelerates Osteogenic Transformation of Human Dermal Fibroblasts by Enhancing Cell Pluripotency. 低强度纳秒脉冲电场通过增强细胞多能性促进人真皮成纤维细胞成骨转化。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1089/cell.2023.0059

Jingtian Lai, Zewei Wang, Haiying Zhou, Pengfei Li, Hui Lu, Tian Tu

{"title":"Low-Intensity Nanosecond Pulsed Electric Field Accelerates Osteogenic Transformation of Human Dermal Fibroblasts by Enhancing Cell Pluripotency.","authors":"Jingtian Lai, Zewei Wang, Haiying Zhou, Pengfei Li, Hui Lu, Tian Tu","doi":"10.1089/cell.2023.0059","DOIUrl":"10.1089/cell.2023.0059","url":null,"abstract":"Autologous human fibroblasts have the potential to differentiate into the osteogenic lineage under specific conditions and can be utilized for bone regeneration. However, their efficiency is currently unsatisfactory. Recently, low-intensity nanosecond pulsed electric field (nsPEF) stimulation has been demonstrated to enhance cell pluripotency by activating epigenetic regulatory pathways. In this study, human dermal fibroblasts were exposed to different intensities of nsPEF to assess whether these exposures resulted in changes in proliferation rate, calcium salt deposition, and expression of differentiation-related markers in different experimental groups. The results showed a significant increase in cell proliferation, pluripotency, bone marker expression, and osteogenic differentiation efficiency when stimulating cells with 5 kV/cm of nsPEF. However, cell proliferation and differentiation significantly decreased at 25 kV/cm. Additionally, the proliferation and efficiency of osteogenic differentiation were reduced when the nsPEF intensity was increased to 50 kV/cm. Treatment with a 5 kV/cm of nsPEF led to increased and concentrated expression of Yes-Associated Protein (YAP) in the nucleus. These observations suggest that human dermal fibroblasts possess a heightened potential to differentiate into osteogenic cells when activated with nsPEF at 5 kV/cm. Consequently, the nsPEF strengthening strategy shows promise for fibroblast-based tissue-engineered bone repair research.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"300-309"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Regulatory Networks: Improving Inferences with Transfer Learning. 基因调控网络：利用迁移学习改进推断。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 DOI: 10.1089/cell.2023.0095

Marcelo Tigre Moura

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