Cellular reprogramming最新文献

{"title":"Silver Nanoparticles Improve the Biocompatibility and Reduce the Immunogenicity of Xenogeneic Scaffolds Derived from Decellularized Pancreas.","authors":"Hongquan Qiu,&nbsp;Liang Zhang,&nbsp;Dongzhi Wang,&nbsp;Haiyan Miao","doi":"10.1089/cell.2021.0071","DOIUrl":"https://doi.org/10.1089/cell.2021.0071","url":null,"abstract":"<p><p>Xenogeneic scaffolds derived from the decellularized pancreas are plausible biomedical materials for pancreatic tissue engineering applications. During the decellularized process, the ultrastructure of extracellular matrices, including collagen fibers, was destructed, which leads to the decrease of mechanical strength and the immune-inflammatory response after transplantation <i>in vivo</i>. The cross-linking method plays an important role in increasing mechanical strength and reducing the inflammatory potential of decellularized scaffolds. However, no ideal cross-linking agent has been identified for decellularized pancreatic scaffolds yet. In this study, a cyclic perfusion system was used to cross-link decellularized pancreatic scaffolds from Sprague Dawley rat with silver nanoparticles (AgNPs). The optimum concentration of AgNPs was selected according to the scanning electron microscope observation and mechanical evaluation, as well as cytotoxicity to human umbilical vein endothelial cells and MIN-6 cell lines <i>in vitro</i>. The inflammation after transplantation <i>in vivo</i> was evaluated by hematoxylin and eosin staining; M1/M2 polarization phenotype of macrophages was further evaluated. Our results showed that after cross-linking, the scaffold possessed better mechanical property and biocompatibility, with the polarization of M2 macrophages increased. Thus, AgNP-cross-linked pancreatic acellular scaffold can provide an ideal scaffold source for pancreatic tissue engineering.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 1","pages":"38-47"},"PeriodicalIF":1.6,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39888844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>POU5F1</i> Protein and Gene Expression Analysis in Neonate and Adult Mouse Testicular Germ Cells by Immunohistochemistry and Immunocytochemistry.","authors":"Parisa Niknejad,&nbsp;Hossein Azizi,&nbsp;Kiana Sojoudi","doi":"10.1089/cell.2021.0108","DOIUrl":"https://doi.org/10.1089/cell.2021.0108","url":null,"abstract":"<p><p>POU5F1 (POU class 5 homeobox 1) is a transcription factor that is critically involved in the self-renewal of undifferentiated embryonic stem cells. In this present study, we have developed our study to analyze the expression of the POU5F1 in the neonatal and adult mice testis section and isolated spermatogonial stem cells (SSCs). We also examine POU5F1 protein localization by three various kinds of antibodies. In this experimental research, to enhance our understanding of the POU5F1 expression levels, protein localization, and function in testicular germ cell, we used immunohistochemistry, immunocytochemistry, and Fluidigm real-time polymerase chain reaction (RT-PCR) analysis in the mouse testis section and neonatal and adult SSCs, and also we used protein-protein network analysis and gene enrichment analysis for genes involved in testicular development. Counting POU5F1-positive cells represented significantly higher expression (<i>p</i> < 0.05) of POU5F1 in the adult testis in comparison to the neonate. Finally, Fluidigm RT-PCR showed a significant expression (<i>p</i> < 0.05) level of germ cells gene <i>POU5F1</i> in neonate SSCs (1-2 week) than 16-24 week SSCs. The illustrated results identify POU5F1 as a necessary transcription factor of testicular germ cells and can be supportive for the investigation of the development and differentiation of SSCs.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 6","pages":"349-358"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39720598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Development of Umbilical Cord-Derived Mesenchymal Stem Cells During Long-Term Maintenance in Fetal Bovine Serum-Supplemented Medium and Xeno- and Serum-Free Culture Medium.","authors":"Hang Minh Le,&nbsp;Lung Tien Nguyen,&nbsp;Diem Huong Hoang,&nbsp;Trung Quoc Bach,&nbsp;Ha Thi Ngoc Nguyen,&nbsp;Hien Thi Mai,&nbsp;Dong Phuong Trinh,&nbsp;Tu Dac Nguyen,&nbsp;Liem Thanh Nguyen,&nbsp;Uyen Thi Trang Than","doi":"10.1089/cell.2021.0050","DOIUrl":"https://doi.org/10.1089/cell.2021.0050","url":null,"abstract":"<p><p>Umbilical cord-derived mesenchymal stem/stromal cells (UC-MSCs) are believed to have potential for the treatment of various diseases; thus, many scientists have investigated the molecular mechanisms underlying the function of UC-MSCs and, for example, the appropriate media for large-scale UC-MSC expansion to prepare cells for real-world application. In this study, we investigated the cellular morphology, proliferation capacity, surface markers, cellular senescence signals, clonogenic potential, trilineage differentiation capacity, and secreted factors of human primary UC-MSCs in long-term culture from passage 2 (P2) to passage 10 (P10) with either conventional fetal bovine serum (FBS)-supplemented medium or commercial xeno- and serum-free medium (StemMACS™). We found that the cells cultured in both media had similar morphology and marker expression. However, the proliferation kinetics as measured by the cell population doubling time differed in a passage (P2-P10)-dependent manner between the cells cultured in the two media; sustainable growth was observed in cells maintained in xeno- and serum-free medium. Moreover, significant differences in cellular senescence signals were observed, with more aging cells in the cell population cultured in FBS-containing medium. Colony numbers and the day that the first colony appeared were similar; however, UC-MSC colony sizes were smaller when cultured in FBS-containing medium. In addition, the multidifferentiation potential of UC-MSCs cultured in xeno- and serum-free StemMACS medium was maintained during long-term culture, but this potential was lost for adipogenic differentiation at P9. Moreover, secreted epidermal growth factor and vascular endothelial growth factor (VEGF)-A were detected in the conditioned media from UC-MSCs, whereas platelet-derived growth factor was not. Similar expression of these factors was observed in conditioned media of UC-MSCs cultured in StemMACS, but the VEGF level was higher in young UC-MSCs (P6) than in aged UC-MSCs cultured in FBS-supplemented Dulbecco's modified Eagle's medium/F12. Thus, StemMACS is better for UC-MSC expansion than conventional FBS-supplemented culture medium, especially when culturing UC-MSCs for real-world applications.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 6","pages":"359-369"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39853601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Cytokine Profile in Mesenchymal Stem Cells from Human Adipose, Umbilical Cord, and Placental Tissues.","authors":"Kun Zhang,&nbsp;Fang Li,&nbsp;Bing Yan,&nbsp;Dong-Jie Xiao,&nbsp;Yun-Shan Wang,&nbsp;Hua Liu","doi":"10.1089/cell.2021.0043","DOIUrl":"https://doi.org/10.1089/cell.2021.0043","url":null,"abstract":"<p><p>Human mesenchymal stem cells (MSCs) can be isolated from various tissues. However, the cytokine profile in different MSC types remains unclear. In this study, MSCs were extracted from adipose, umbilical cord, and placental tissues. The surface marker expression, multilineage differentiation potential, and cytokine secretion of these cells were compared. The isolated MSCs exhibited similar morphology and surface marker expression. However, they differed with regard to their differentiation potential. Adipose-MSCs (A-MSCs) exhibited a higher potential for adipogenesis and osteogenic differentiation compared with umbilical cord-MSCs (UC-MSCs) and placental-MSCs (P-MSCs). The expression levels of 80 cytokines were detected, and the data demonstrated that the three MSC types abundantly secreted insulin-like growth factor-binding protein (IGFBP)-4, IGFBP-3, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, IGFBP-6, monocyte chemoattractant protein-1, and granulocyte colony-stimulating factor. However, the expression levels of vascular endothelial growth factor, tumor necrosis factor alpha, interleukin (IL)-6 receptor, and IL-13 in A-MSCs were higher compared with those of UC-MSCs and P-MSCs. Moreover, the expression levels of intercellular adhesion molecule-1 and growth differentiation factor 15 were lower in A-MSCs. Kyoto Encyclopedia of Genes and Genomes analysis indicated that the \"adipocytokine\" and the \"PI3K/Akt pathways\" were enriched in A-MSCs. Taken together, the results demonstrated that MSCs from different sources exhibited differences in the secretion of specific factors. A-MSCs were associated with the expression of several proangiogenic factors and may be an improved source for angiogenesis and tissue regeneration.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 6","pages":"336-348"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39539668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Derivation of Ringed Seal (<i>Phoca hispida</i>) Induced Multipotent Stem Cells.","authors":"Violetta R Beklemisheva,&nbsp;Polina S Belokopytova,&nbsp;Veniamin S Fishman,&nbsp;Aleksei G Menzorov","doi":"10.1089/cell.2021.0037","DOIUrl":"https://doi.org/10.1089/cell.2021.0037","url":null,"abstract":"<p><p>Induced pluripotent stem (iPS) cells have been produced just for a few species among order Carnivora: snow leopard, Bengal tiger, serval, jaguar, cat, dog, ferret, and American mink. We applied the iPS cell derivation protocol to the ringed seal (<i>Phoca hispida</i>) fibroblasts. The resulting cell line had the expression of pluripotency marker gene <i>Rex1</i>. Differentiation in embryoid body-like structures allowed us to register expression of <i>AFP</i>, endoderm marker, and <i>Cdx2</i>, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably is not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipocytes and osteocytes.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 6","pages":"326-335"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39740952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming Stars #4: A Reprogramming Approach for Parkinson's Disease-An Interview with Dr. Malin Parmar.","authors":"Malin Parmar,&nbsp;Carlos-Filipe Pereira","doi":"10.1089/cell.2021.29049.mp","DOIUrl":"https://doi.org/10.1089/cell.2021.29049.mp","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 6","pages":"319-325"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39711278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cellular Reprogramming</i> Call for Papers: Special Issue on Direct Cell Reprogramming.","authors":"","doi":"10.1089/cell.2021.29042.malcfp2","DOIUrl":"https://doi.org/10.1089/cell.2021.29042.malcfp2","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 5","pages":"263"},"PeriodicalIF":1.6,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39396073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reprogramming Stars #3: Mechanisms of iPSC Reprogramming-An Interview with Dr. Keisuke Kaji.","authors":"Keisuke Kaji,&nbsp;Carlos-Filipe Pereira","doi":"10.1089/cell.2021.29046.kk","DOIUrl":"https://doi.org/10.1089/cell.2021.29046.kk","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 5","pages":"264-269"},"PeriodicalIF":1.6,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39418742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin-10 Modulates the Metabolism and Osteogenesis of Human Dental Pulp Stem Cells.","authors":"Li Yuan,&nbsp;Hongxia You,&nbsp;Nianhong Qin,&nbsp;Wenxin Zuo","doi":"10.1089/cell.2021.0044","DOIUrl":"https://doi.org/10.1089/cell.2021.0044","url":null,"abstract":"<p><p>The osteogenic differentiation of mesenchymal stem cells (MSCs) is strongly related with the inflammatory microenvironment. The ability of osteogenic differentiation of MSCs is vital for the bone tissue engineering. Interleukin (IL)-10, a well-known anti-inflammatory factor, plays a key role in tissue repair. Dental pulp stem cells (DPSCs), with the advantage of convenience of extraction, are suitable for the bone tissue engineering. Therefore, it is meaning to explore the effects of IL-10 on the osteogenic differentiation of DPSCs. The proliferation activity of DPSCs were evaluated by MTS assay (CellTiter 96^® Aqueous One Solution Cell Proliferation Assay [Promega]) and real-time polymerase chain reaction (RT-PCR). The osteogenic differentiation of DPSCs were determined by Alizarin Red staining, RT-PCR, and alkaline phosphatase activity test. The glucose metabolism was detected by Mito Stress test and glycolysis assay. IL-10 (10 or 20 nM) could enhance the osteogenic differentiation of DPSCs and promoted the metabolic switch from glycolysis to oxidative phosphorylation (OXPHOS), whereas IL-10 (5 and 50 nM) has no obvious effects on the osteogenic differentiation of DPSCs. The OXPHOS inhibitor restrained the promotion of osteogenic differentiation induced by IL-10. These findings show that IL-10 can promote the osteogenesis of DPSCs through the activation of OXPHOS, which provides a potential way for enhancing the osteogenic differentiation of DPSCs in bone tissue engineering.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 5","pages":"270-276"},"PeriodicalIF":1.6,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39394368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of Urine-Derived Induced Pluripotent Stem Cell Line from Patients with Acute Kidney Injury.","authors":"Yong Jin,&nbsp;Manling Zhang,&nbsp;Meishuang Li,&nbsp;Hao Zhang,&nbsp;Feng Zhang,&nbsp;Hong Zhang,&nbsp;Zhibao Yin,&nbsp;Meng Zhou,&nbsp;Xin Wan,&nbsp;Rongfeng Li,&nbsp;Changchun Cao","doi":"10.1089/cell.2021.0051","DOIUrl":"https://doi.org/10.1089/cell.2021.0051","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is mainly characterized by rapid decline of renal function. Currently, the strategy of stem cells might be a therapy to treat AKI. The objective of this study was to obtain human urine-derived cells (HUCs) from patients with AKI, followed by establishing induced pluripotent stem (iPS) cell line. We isolated urine cells from patients with AKI and found that the cells could survive long term with epithelioid morphology and maintain a normal karyotype. The cell line had expression of renal-specific markers and renal development-related genes. After induction, the urine cells cotransfecting with TET-ON vectors were converted into iPS cells. The HUC-derived iPS (HUC-iPS) was positive for alkaline phosphatase staining, and had expression of pluripotency markers, consistent with human embryonic fibroblast-derived iPS cell. Notably, HUC-iPS could be induced to undergo directional kidney precursor cells (KPCs) differentiation under defined conditions, and transplantation of KPCs resulted in reducing kidney damage from ischemia-reperfusion injury in mice. Therefore, we successfully established HUC-iPS cell from patients with AKI and provided a novel stem cell resource for cell therapy in AKI.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"23 5","pages":"290-303"},"PeriodicalIF":1.6,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39542316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}