Cellular reprogramming最新文献_第10页

Call for Special Issue Papers: Cellular Reprogramming 25th Anniversary Deadline for Manuscript Submission: April 30, 2023. 特刊论文征集:细胞重编程25周年论文提交截止日期:2023年4月30日。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.29073.cfp

Carlos-Filipe Pereira

引用次数: 0

Direct Reprogramming Retains Aging Signatures That Are Critical to Reveal Parkinson's Disease-Associated Autophagy Phenotypes. 直接重编程保留衰老特征是揭示帕金森病相关自噬表型的关键

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0127

Elezabeth Stephen, Heather Mortiboys

引用次数: 0

Fluorescent Reporters Distinguish Stem Cell Colony Subtypes During Somatic Cell Reprogramming. 在体细胞重编程过程中用荧光报告区分干细胞集落亚型

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 Epub Date: 2022-11-03 DOI: 10.1089/cell.2022.0071

Alexandra Moauro, Robin E Kruger, Daniel O'Hagan, Amy Ralston

{"title":"Fluorescent Reporters Distinguish Stem Cell Colony Subtypes During Somatic Cell Reprogramming.","authors":"Alexandra Moauro, Robin E Kruger, Daniel O'Hagan, Amy Ralston","doi":"10.1089/cell.2022.0071","DOIUrl":"10.1089/cell.2022.0071","url":null,"abstract":"Somatic cell reprogramming was first developed to create induced pluripotent stem (iPS) cells. Since that time, the highly dynamic and heterogeneous nature of the reprogramming process has come to be appreciated. Remarkably, a distinct type of stem cell, called induced extraembryonic endoderm (iXEN) stem cell, is also formed during reprogramming of mouse somatic cells by ectopic expression of the transcription factors, OCT4, SOX2, KLF4, and MYC (OSKM). The mechanisms leading somatic cells to adopt differing stem cell fates are challenging to resolve given that formation of either stem cell type is slow, stochastic, and rare. For these reasons, fluorescent gene expression reporters have provided an invaluable tool for revealing the path from the somatic state to pluripotency. However, no such reporters have been established for comparable studies of iXEN cell formation. In this study, we examined the expression of multiple fluorescent reporters, including Nanog, Oct4, and the endodermal genes, Gata4 and Gata6-alone and in combination, during reprogramming. We show that only simultaneous evaluation of Nanog and Gata4 reliably distinguishes iPS and iXEN cell colonies during reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 6","pages":"353-362"},"PeriodicalIF":1.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/1e/cell.2022.0071.PMC9805857.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9080971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Tumor Microenvironment Reprograms Immune Cells. 肿瘤微环境重编程免疫细胞。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0047

Handi Cao, Sanxing Gao, Ritika Jogani, Ryohichi Sugimura

引用次数: 3

Reprogramming Stars #9: Spacing Out Cellular Reprogramming-An Interview with Dr. Valentina Fossati. 重编程之星 #9：间隔细胞重编程--专访瓦伦蒂娜-福萨蒂博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 Epub Date: 2022-11-21 DOI: 10.1089/cell.2022.29074.vf

Valentina Fossati, Carlos-Filipe Pereira

引用次数: 0

The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus. 间充质干细胞治疗糖尿病的潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-12-01 DOI: 10.1089/cell.2022.0039

Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun

{"title":"The Therapeutic Potential of Mesenchymal Stem Cells in the Treatment of Diabetes Mellitus.","authors":"Liang Zhu, Sheng Wang, JunSheng Qu, Zongguang Hui, Chengxia Kan, Ningning Hou, Xiaodong Sun","doi":"10.1089/cell.2022.0039","DOIUrl":"https://doi.org/10.1089/cell.2022.0039","url":null,"abstract":"Mesenchymal stem cells (MSCs) exist in many tissues and can differentiate into cells of multiple lineages, such as adipocytes, osteoblasts, or chondrocytes. MSC administration has demonstrated therapeutic potential in various degenerative and inflammatory diseases (e.g., graft-vs.-host disease, multiple sclerosis, Crohn's disease, organ fibrosis, and diabetes mellitus [DM]). The mechanisms involved in the therapeutic effects of MSCs are multifaceted. Generally, implanted MSCs can migrate to sites of injury, where they establish an anti-inflammatory and regenerative microenvironment in damaged tissues. In addition, MSCs can modulate innate and adaptive immune responses through immunosuppressive mechanisms that involve immune cells, inflammatory cytokines, chemokines, and immunomodulatory factors. DM has a high prevalence worldwide; it also contributes to a high rate of mortality worldwide. MSCs offer a promising therapeutic agent to prevent or repair damage from DM and diabetic complications through properties such as multilineage differentiation, homing, promotion of angiogenesis, and immunomodulation (e.g., prevention of oxidative stress, fibrosis, and cell death). In this study, we review current findings regarding the immunomodulatory and regenerative mechanisms of MSCs, as well as their therapeutic applications in DM and DM-related complications.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"24 6","pages":"329-342"},"PeriodicalIF":1.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10574455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Synthetic Embryos Can Complete Gastrulation and Initiate Organogenesis Ex Utero. 合成胚胎可以在子宫外完成原肠胚形成并启动器官发生。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 DOI: 10.1089/cell.2022.0111

Alejo E Rodriguez-Fraticelli

引用次数: 0

The 2022 International Society for Stem Cell Research (ISSCR) Annual Meeting: Celebrating 20 Years of Achievements. 2022年国际干细胞研究学会(ISSCR)年会:庆祝20年的成就。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 DOI: 10.1089/cell.2022.0105

Janelle Drouin-Ouellet, Dan Li, Yuancheng Ryan Lu, Camila Vazquez Echegaray

引用次数: 0

Past, Present, and Future of Direct Cell Reprogramming. 直接细胞重编程的过去、现在和未来。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 DOI: 10.1089/cell.2022.0110

Henrik Ahlenius

引用次数: 2

Appropriate Exogenous Expression Stoichiometry of GATA4 as an Important Factor for Cardiac Reprogramming of Human Dermal Fibroblasts. 人皮肤成纤维细胞心脏重编程的重要因子——GATA4的适当外源性表达化学计量。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2022-10-01 Epub Date: 2022-06-28 DOI: 10.1089/cell.2022.0014

Xiangyu Zhang, Qi Zhang, Lijun Chen, Baomei Cai, Mengying Zeng, Sihua Ou, Yating Chen, Ziyu Feng, Huan Chen, Shangtao Cao, Kai Kang

{"title":"Appropriate Exogenous Expression Stoichiometry of GATA4 as an Important Factor for Cardiac Reprogramming of Human Dermal Fibroblasts.","authors":"Xiangyu Zhang, Qi Zhang, Lijun Chen, Baomei Cai, Mengying Zeng, Sihua Ou, Yating Chen, Ziyu Feng, Huan Chen, Shangtao Cao, Kai Kang","doi":"10.1089/cell.2022.0014","DOIUrl":"https://doi.org/10.1089/cell.2022.0014","url":null,"abstract":"Reprogramming of human dermal fibroblasts (HDFs) into induced cardiomyocyte-like cells (iCMs) represents a promising strategy for human cardiac regeneration. Different cocktails of cardiac transcription factors can convert HDFs into iCMs, although with low efficiency and immature phenotype. Here, GATA4, MEF2C, TBX5, MESP1, and MYOCD (GMTMeMy for short) were used to reprogram HDFs by retrovirus infection. We found that the exogenous expression stoichiometry of GATA4 (GATA4 stoichiometry) significantly affected reprogramming efficiency. When 1/8 dosage of GATA4 virus (GATA4 dosage) plus MTMeMy was used, the reprogramming efficiency was obviously improved compared with average pooled virus encoding each factor, which measured, by the expression level of cardiac genes, the percentage of cardiac troponin T and alpha-cardiac myosin heavy-chain immunopositive cells and the numbers of iCMs showing calcium oscillation or beating synchronously in co-culture with mouse CMs. In addition, we prepared conditioned maintenance medium (CMM) by CM differentiation of H9 human embryonic stem cell line. We found that compared with traditional maintenance medium (TMM), CMM made iCMs show well-organized sarcomere formation and characteristic calcium oscillation wave earlier. These findings demonstrated that appropriate GATA4 stoichiometry was essential for cardiac reprogramming and some components in CMM were important for maturation of iCMs.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"283-293"},"PeriodicalIF":1.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40405502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0