Cellular reprogramming最新文献_第10页

A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation. 一种分离和冷冻保存人脂肪干细胞的新方法

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0017

Young-Cheol Lim, Jung-Il Jung, In-Kee Hong

{"title":"A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation.","authors":"Young-Cheol Lim, Jung-Il Jung, In-Kee Hong","doi":"10.1089/cell.2023.0017","DOIUrl":"https://doi.org/10.1089/cell.2023.0017","url":null,"abstract":"Adipose-derived stem cells (ADSCs) are isolated from abundant adipose tissue and have the capacity to differentiate into multiple cell lineages. ADSCs have raised big interest in therapeutic applications in regenerative medicine and demonstrated to fulfill the criteria for a successful cell therapy. There are several methods for isolation of ADSCs from adipose tissue and cryopreservation of ADSCs. Here, novel methods for the isolation and cryopreservation of ADSCs are presented and focused. Microscopic pieces of adipose tissue were placed on transwell inserts, and the ADSCs were induced to migrate to the lower wells for 1 week. We compared the properties of our ADSCs with those isolated by enzymatic digestion and enzyme-free method of culture plate, and our ADSCs were found to be more stable and healthier. In addition, we proposed a novel cryoprotectant solution (FNCP) containing pectin and L-alanine, which was compared with standard cryoprotectant solution. Overall, our methods proved more useful for ADSCs isolation than other methods and did not require consideration of \"minimal manipulation\" by the U.S. Food and Drug Administration (FDA). Furthermore, our FNCP did not contain dimethyl sulfoxide and fetal bovine serum, therefore stable storage is possible in xeno-free and animal-free cryopreservation solutions.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"171-179"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10138576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Transdifferentiation: A Challenging Strategy with Great Potential. 细胞转分化:一个具有巨大潜力的挑战策略。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0015

Fuping Wang, Runting Li, Limeng Zhang, Xiaoning Nie, Linqing Wang, Longxin Chen

引用次数: 0

A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models. 细胞年轻化的更安全途径:在早衰小鼠模型中通过CRISPR/dCas9内源性Oct4激活

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0057

Di Hu, Enora Le Borgne, Rico Meinl

{"title":"A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models.","authors":"Di Hu, Enora Le Borgne, Rico Meinl","doi":"10.1089/cell.2023.0057","DOIUrl":"https://doi.org/10.1089/cell.2023.0057","url":null,"abstract":"A recent study in Aging Cell showed that transcriptional activation of endogenous Oct4 using the CRISPR/dCas9 activator system is sufficient for cellular rejuvenation and extending the lifespan of a progeria mouse model. Although transient expression of reprogramming factors Oct4, Sox2, Klf4, and c-Myc (OSKM) has been shown to ameliorate age-related phenotypes in vivo, oncogenic risk, for example, from c-Myc, has raised safety concerns for its use in therapeutics. The authors demonstrated that transient activation of endogenous Oct4 expression restored age-related epigenetic patterns, suppressed expression of mutant progerin, and reduced vascular pathological features associated with the disease. At the same time, the transient Oct4 overexpression resulted in lower incidence of cancer transformation compared with constituent OSKM overexpression. Successful activation of endogenous Oct4 by CRISPR/dCas9 paves the way for novel therapeutic approaches for the treatment of progeria and age-related diseases, with potential implications for the broader field of cellular reprogramming-based rejuvenation.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"136-138"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Emerging Role of B1 SINE in Pluripotent Reprogramming. B1 SINE 在多能性重编程中的新作用。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-08 DOI: 10.1089/cell.2023.0037

Jere Weltner, Ras Trokovic

引用次数: 0

Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment. 肿瘤微环境中糖酵解代谢关键酶的功能

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-12 DOI: 10.1089/cell.2023.0010

Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen

{"title":"Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment.","authors":"Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen","doi":"10.1089/cell.2023.0010","DOIUrl":"10.1089/cell.2023.0010","url":null,"abstract":"The tumor microenvironment (TME) plays a crucial role in tumor initiation, growth and metastasis. Metabolic enzymes involved in tumor glycolytic reprogramming, including hexokinase, pyruvate kinase, and lactate dehydrogenase, not only play key roles in tumorigenesis and maintaining tumor cell survival, but also take part in the modulation of the TME. Many studies have been devoted to the role of key glycolytic enzymes in the TME over the past decades. We summarize the studies on the role of glycolytic enzymes in the TME of these years and found that glycolytic enzymes remodel the TME primarily through regulating immune escape, angiogenesis, and affecting stromal cells and exosomes. Notably, abnormal tumor vascular system, peritumoral stromal cells, and tumor immunosuppressive microenvironment are important contributors to the failure of antitumor therapy. Therefore, we discuss the mechanisms of regulation by key glycolytic enzymes that may contribute to a promising biomarker for therapeutic intervention. We argue that targeting key glycolytic enzymes in combination with antiprogrammed cell death ligand 1 or antivascular endothelial growth factor could emerge as the more integrated and comprehensive antitumor treatment strategy.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"91-98"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9704895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Effects of Extracellular Vesicles Derived from Mesenchymal Stem Cells on Apoptosis and Differentiation of Osteoblasts Treated with CoCl₂. 间充质干细胞衍生的细胞外囊泡对受 CoCl2 处理的成骨细胞凋亡和分化的改善作用

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-15 DOI: 10.1089/cell.2023.0001

Qicheng Li, Wei Zhang, Jin Deng, Qiuya Li, Xiaoyang Fu, Yuhui Kou, Na Han

{"title":"Ameliorative Effects of Extracellular Vesicles Derived from Mesenchymal Stem Cells on Apoptosis and Differentiation of Osteoblasts Treated with CoCl2.","authors":"Qicheng Li, Wei Zhang, Jin Deng, Qiuya Li, Xiaoyang Fu, Yuhui Kou, Na Han","doi":"10.1089/cell.2023.0001","DOIUrl":"10.1089/cell.2023.0001","url":null,"abstract":"Severe osteoporotic fracture occurring in sites with inadequate blood supply can cause irreversible damage to cells, particularly osteoblasts, with current drug and surgical interventions exhibiting limitations for elderly individuals. As participants mediating intercellular communication, extracellular vesicles (EVs) are rarely reported to play functional roles in osteoblasts under hypoxia. Our study mainly investigated the effects of bone marrow mesenchymal stem cells-derived EVs (BMSCs-EVs) on apoptosis and differentiation of osteoblasts treated with CoCl2. Primary rat BMSCs and osteoblasts were extracted as required for the following experiments. Cell counting kit 8 assay was used to explore the concentration of CoCl2 for treating osteoblasts, and we found that 100 μM CoCl2 was appropriate to treat osteoblasts for 48 hours. The analysis of flow cytometer showed that CoCl2-treated osteoblasts apoptosis can be ameliorated when cocultured with BMSCs-EVs. Further findings revealed that reactive oxygen species (ROS) was related to CoCl2-induced apoptosis. In addition, our results demonstrated that EVs exerted an important role in increasing expression levels of ALP, BMP-2, OCN, and OSTERIX under hypoxia. Similarly, the functional effects of BMSCs-EVs were observed on the osteoblasts mineralization. In summary, these findings provide insight that BMSCs-EVs might decrease the effect of CoCl2-induced apoptosis through inhibiting ROS, and promote osteogenic differentiation under hypoxia.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"99-108"},"PeriodicalIF":1.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of MSTN Gene-Edited Embryos of Buffalo Using the CRISPR/Cas9 System and SCNT. 利用 CRISPR/Cas9 系统和 SCNT 生产水牛 MSTN 基因编辑胚胎。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-04-11 DOI: 10.1089/cell.2023.0003

Seema Dua, Sonu Bansal, Devika Gautam, Bosco Jose, Priyanka Singh, Manoj Kumar Singh, Sachinandan De, Dharmendra Kumar, Prem Singh Yadav, Wilfried Kues, Naresh L Selokar

{"title":"Production of MSTN Gene-Edited Embryos of Buffalo Using the CRISPR/Cas9 System and SCNT.","authors":"Seema Dua, Sonu Bansal, Devika Gautam, Bosco Jose, Priyanka Singh, Manoj Kumar Singh, Sachinandan De, Dharmendra Kumar, Prem Singh Yadav, Wilfried Kues, Naresh L Selokar","doi":"10.1089/cell.2023.0003","DOIUrl":"10.1089/cell.2023.0003","url":null,"abstract":"The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm animal species for improved production and health traits; however, these tools are rarely used in the buffalo and can play a pivotal role in milk and meat production in tropical and subtropical countries. In this study, we aimed to produce myostatin (MSTN) gene-edited embryos of the Murrah buffalo using the CRISPR/Cas9 system and SCNT. For this, fibroblast cells were electroporated with sgRNAs carrying all-in-one CRISPR/Cas9 plasmids targeting the first exon of the MSTN gene. Following puromycin selection, single-cell clonal populations were established and screened using the TA cloning and Sanger sequencing methods. Of eight single-cell clonal populations, one with a monoallelic and another with a biallelic heterozygous gene editing event were identified. These two gene-edited clonal cell populations were successfully used to produce blastocyst-stage embryos using the handmade cloning method. This work establishes the technical foundation for generation of genome-edited cloned embryos in the buffalo.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"121-127"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #12: At the Heart of In Vivo Reprogramming- An Interview with Dr. Li Qian. 重编程之星#12:体内重编程的核心——李茜博士访谈录

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 DOI: 10.1089/cell.2023.0056

Li Qian, Carlos-Filipe Pereira

引用次数: 0

MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells. 过表达 MicroRNA26a 可加快牙科干细胞的成骨细胞分化能力

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-18 DOI: 10.1089/cell.2023.0004

Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar

{"title":"MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells.","authors":"Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar","doi":"10.1089/cell.2023.0004","DOIUrl":"10.1089/cell.2023.0004","url":null,"abstract":"Dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) are a source of mesenchymal stem cells with the potential to differentiate into several cell types. We initially isolated SHED cells and compared their osteogenic capacity with commercially available DPSCs. Both cells exhibited similar capacities of growth and osteogenic differentiation. A fourfold to sixfold increase in endogenous microRNA26a (miR26a) expression during osteogenic differentiation of preosteoblasts and a similar but attenuated increase (twofold to fourfold) in differentiating SHED was observed, suggesting a role in the process. We, therefore, overexpressed miR26a in SHED to determine if the osteogenic differentiation capacity can be potentiated in vitro. SHED with a threefold increase in miR26a expression showed increased growth rate when compared with parent cells. When exposed to an osteogenic differentiating promoting medium, the miR26a overexpressing cells showed 100-fold increases in the expression of bone marker genes such as type 1 collagen, alkaline phosphatase, and Runx2. The mineralization capacity of these cells was also increased 15-fold. As miR26a targets regulate several bone-specific genes, we evaluated the effect of miR26a overexpression on established targets. We found a moderate decrease in SMAD1 and a profound decrease in PTEN expression. miR26a could potentiate its effect on osteoblast differentiation by its ability to inhibit PTEN and increase the viability of cells and their numbers, a process essential in osteoblast differentiation. Our studies suggest that the upregulation of miR26a can increase bone formation and may serve as an important target to further investigate its potential in tissue engineering applications.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"109-120"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9705422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of All Human Transcription Factors on the Directed Differentiation of Pluripotent Stem Cells. 所有人转录因子对多能干细胞定向分化的影响。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2023.0007

Rodrigo L Dos Santos

引用次数: 0