Cellular reprogramming最新文献_第8页

A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models. 细胞年轻化的更安全途径:在早衰小鼠模型中通过CRISPR/dCas9内源性Oct4激活

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0057

Di Hu, Enora Le Borgne, Rico Meinl

{"title":"A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models.","authors":"Di Hu, Enora Le Borgne, Rico Meinl","doi":"10.1089/cell.2023.0057","DOIUrl":"https://doi.org/10.1089/cell.2023.0057","url":null,"abstract":"A recent study in Aging Cell showed that transcriptional activation of endogenous Oct4 using the CRISPR/dCas9 activator system is sufficient for cellular rejuvenation and extending the lifespan of a progeria mouse model. Although transient expression of reprogramming factors Oct4, Sox2, Klf4, and c-Myc (OSKM) has been shown to ameliorate age-related phenotypes in vivo, oncogenic risk, for example, from c-Myc, has raised safety concerns for its use in therapeutics. The authors demonstrated that transient activation of endogenous Oct4 expression restored age-related epigenetic patterns, suppressed expression of mutant progerin, and reduced vascular pathological features associated with the disease. At the same time, the transient Oct4 overexpression resulted in lower incidence of cancer transformation compared with constituent OSKM overexpression. Successful activation of endogenous Oct4 by CRISPR/dCas9 paves the way for novel therapeutic approaches for the treatment of progeria and age-related diseases, with potential implications for the broader field of cellular reprogramming-based rejuvenation.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"136-138"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Emerging Role of B1 SINE in Pluripotent Reprogramming. B1 SINE 在多能性重编程中的新作用。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-08 DOI: 10.1089/cell.2023.0037

Jere Weltner, Ras Trokovic

引用次数: 0

Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment. 肿瘤微环境中糖酵解代谢关键酶的功能

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-12 DOI: 10.1089/cell.2023.0010

Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen

{"title":"Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment.","authors":"Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen","doi":"10.1089/cell.2023.0010","DOIUrl":"10.1089/cell.2023.0010","url":null,"abstract":"The tumor microenvironment (TME) plays a crucial role in tumor initiation, growth and metastasis. Metabolic enzymes involved in tumor glycolytic reprogramming, including hexokinase, pyruvate kinase, and lactate dehydrogenase, not only play key roles in tumorigenesis and maintaining tumor cell survival, but also take part in the modulation of the TME. Many studies have been devoted to the role of key glycolytic enzymes in the TME over the past decades. We summarize the studies on the role of glycolytic enzymes in the TME of these years and found that glycolytic enzymes remodel the TME primarily through regulating immune escape, angiogenesis, and affecting stromal cells and exosomes. Notably, abnormal tumor vascular system, peritumoral stromal cells, and tumor immunosuppressive microenvironment are important contributors to the failure of antitumor therapy. Therefore, we discuss the mechanisms of regulation by key glycolytic enzymes that may contribute to a promising biomarker for therapeutic intervention. We argue that targeting key glycolytic enzymes in combination with antiprogrammed cell death ligand 1 or antivascular endothelial growth factor could emerge as the more integrated and comprehensive antitumor treatment strategy.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"91-98"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9704895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ameliorative Effects of Extracellular Vesicles Derived from Mesenchymal Stem Cells on Apoptosis and Differentiation of Osteoblasts Treated with CoCl₂. 间充质干细胞衍生的细胞外囊泡对受 CoCl2 处理的成骨细胞凋亡和分化的改善作用

IF 1.2 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-15 DOI: 10.1089/cell.2023.0001

Qicheng Li, Wei Zhang, Jin Deng, Qiuya Li, Xiaoyang Fu, Yuhui Kou, Na Han

{"title":"Ameliorative Effects of Extracellular Vesicles Derived from Mesenchymal Stem Cells on Apoptosis and Differentiation of Osteoblasts Treated with CoCl2.","authors":"Qicheng Li, Wei Zhang, Jin Deng, Qiuya Li, Xiaoyang Fu, Yuhui Kou, Na Han","doi":"10.1089/cell.2023.0001","DOIUrl":"10.1089/cell.2023.0001","url":null,"abstract":"Severe osteoporotic fracture occurring in sites with inadequate blood supply can cause irreversible damage to cells, particularly osteoblasts, with current drug and surgical interventions exhibiting limitations for elderly individuals. As participants mediating intercellular communication, extracellular vesicles (EVs) are rarely reported to play functional roles in osteoblasts under hypoxia. Our study mainly investigated the effects of bone marrow mesenchymal stem cells-derived EVs (BMSCs-EVs) on apoptosis and differentiation of osteoblasts treated with CoCl2. Primary rat BMSCs and osteoblasts were extracted as required for the following experiments. Cell counting kit 8 assay was used to explore the concentration of CoCl2 for treating osteoblasts, and we found that 100 μM CoCl2 was appropriate to treat osteoblasts for 48 hours. The analysis of flow cytometer showed that CoCl2-treated osteoblasts apoptosis can be ameliorated when cocultured with BMSCs-EVs. Further findings revealed that reactive oxygen species (ROS) was related to CoCl2-induced apoptosis. In addition, our results demonstrated that EVs exerted an important role in increasing expression levels of ALP, BMP-2, OCN, and OSTERIX under hypoxia. Similarly, the functional effects of BMSCs-EVs were observed on the osteoblasts mineralization. In summary, these findings provide insight that BMSCs-EVs might decrease the effect of CoCl2-induced apoptosis through inhibiting ROS, and promote osteogenic differentiation under hypoxia.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"99-108"},"PeriodicalIF":1.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of MSTN Gene-Edited Embryos of Buffalo Using the CRISPR/Cas9 System and SCNT. 利用 CRISPR/Cas9 系统和 SCNT 生产水牛 MSTN 基因编辑胚胎。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-04-11 DOI: 10.1089/cell.2023.0003

Seema Dua, Sonu Bansal, Devika Gautam, Bosco Jose, Priyanka Singh, Manoj Kumar Singh, Sachinandan De, Dharmendra Kumar, Prem Singh Yadav, Wilfried Kues, Naresh L Selokar

{"title":"Production of MSTN Gene-Edited Embryos of Buffalo Using the CRISPR/Cas9 System and SCNT.","authors":"Seema Dua, Sonu Bansal, Devika Gautam, Bosco Jose, Priyanka Singh, Manoj Kumar Singh, Sachinandan De, Dharmendra Kumar, Prem Singh Yadav, Wilfried Kues, Naresh L Selokar","doi":"10.1089/cell.2023.0003","DOIUrl":"10.1089/cell.2023.0003","url":null,"abstract":"The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm animal species for improved production and health traits; however, these tools are rarely used in the buffalo and can play a pivotal role in milk and meat production in tropical and subtropical countries. In this study, we aimed to produce myostatin (MSTN) gene-edited embryos of the Murrah buffalo using the CRISPR/Cas9 system and SCNT. For this, fibroblast cells were electroporated with sgRNAs carrying all-in-one CRISPR/Cas9 plasmids targeting the first exon of the MSTN gene. Following puromycin selection, single-cell clonal populations were established and screened using the TA cloning and Sanger sequencing methods. Of eight single-cell clonal populations, one with a monoallelic and another with a biallelic heterozygous gene editing event were identified. These two gene-edited clonal cell populations were successfully used to produce blastocyst-stage embryos using the handmade cloning method. This work establishes the technical foundation for generation of genome-edited cloned embryos in the buffalo.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"121-127"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #12: At the Heart of In Vivo Reprogramming- An Interview with Dr. Li Qian. 重编程之星#12:体内重编程的核心——李茜博士访谈录

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 DOI: 10.1089/cell.2023.0056

Li Qian, Carlos-Filipe Pereira

引用次数: 0

MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells. 过表达 MicroRNA26a 可加快牙科干细胞的成骨细胞分化能力

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-18 DOI: 10.1089/cell.2023.0004

Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar

{"title":"MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells.","authors":"Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar","doi":"10.1089/cell.2023.0004","DOIUrl":"10.1089/cell.2023.0004","url":null,"abstract":"Dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) are a source of mesenchymal stem cells with the potential to differentiate into several cell types. We initially isolated SHED cells and compared their osteogenic capacity with commercially available DPSCs. Both cells exhibited similar capacities of growth and osteogenic differentiation. A fourfold to sixfold increase in endogenous microRNA26a (miR26a) expression during osteogenic differentiation of preosteoblasts and a similar but attenuated increase (twofold to fourfold) in differentiating SHED was observed, suggesting a role in the process. We, therefore, overexpressed miR26a in SHED to determine if the osteogenic differentiation capacity can be potentiated in vitro. SHED with a threefold increase in miR26a expression showed increased growth rate when compared with parent cells. When exposed to an osteogenic differentiating promoting medium, the miR26a overexpressing cells showed 100-fold increases in the expression of bone marker genes such as type 1 collagen, alkaline phosphatase, and Runx2. The mineralization capacity of these cells was also increased 15-fold. As miR26a targets regulate several bone-specific genes, we evaluated the effect of miR26a overexpression on established targets. We found a moderate decrease in SMAD1 and a profound decrease in PTEN expression. miR26a could potentiate its effect on osteoblast differentiation by its ability to inhibit PTEN and increase the viability of cells and their numbers, a process essential in osteoblast differentiation. Our studies suggest that the upregulation of miR26a can increase bone formation and may serve as an important target to further investigate its potential in tissue engineering applications.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"109-120"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9705422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of All Human Transcription Factors on the Directed Differentiation of Pluripotent Stem Cells. 所有人转录因子对多能干细胞定向分化的影响。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2023.0007

Rodrigo L Dos Santos

引用次数: 0

Treatments of Porcine Nuclear Recipient Oocytes and Somatic Cell Nuclear Transfer-Generated Embryos with Various Reactive Oxygen Species Scavengers Lead to Improvements of Their Quality Parameters and Developmental Competences by Mitigating Oxidative Stress-Related Impacts. 用各种活性氧清除剂处理猪核受体卵母细胞和体细胞核移植胚胎，通过减轻氧化应激相关的影响，改善其质量参数和发育能力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2022.0145

Seung-Hwan Oh, Seung-Eun Lee, Dong-Hun Han, Jae-Wook Yoon, So-Hee Kim, Eun-Seo Lim, Han-Bi Lee, Eun-Young Kim, Se-Pill Park

{"title":"Treatments of Porcine Nuclear Recipient Oocytes and Somatic Cell Nuclear Transfer-Generated Embryos with Various Reactive Oxygen Species Scavengers Lead to Improvements of Their Quality Parameters and Developmental Competences by Mitigating Oxidative Stress-Related Impacts.","authors":"Seung-Hwan Oh, Seung-Eun Lee, Dong-Hun Han, Jae-Wook Yoon, So-Hee Kim, Eun-Seo Lim, Han-Bi Lee, Eun-Young Kim, Se-Pill Park","doi":"10.1089/cell.2022.0145","DOIUrl":"https://doi.org/10.1089/cell.2022.0145","url":null,"abstract":"This study investigated the antioxidant effects of β-cryptoxanthin (BCX), hesperetin (HES), and icariin (ICA), and their effects on in vitro maturation of porcine oocytes and subsequent embryonic development of somatic cell nuclear transfer (SCNT). Treatment with 1 μM BCX (BCX-1) increased the developmental rate of porcine oocytes more than treatment with 100 μM HES (HES-100) or 5 μM ICA (ICA-5). The glutathione level and mRNA expression of antioxidant genes (NFE2L2, SOD1, and SOD2) were more increased in the BCX-1 group than in the HES-100 and ICA-5 groups, while the reactive oxygen species level was more decreased. Moreover, BCX improved the developmental capacity and quality of SCNT embryos. The total cell number, apoptotic cell rate, and development-related gene expression were modulated in the BCX-1 group to enhance embryonic development of SCNT. These results show that the antioxidant effects of BCX enhance in vitro maturation of porcine oocytes and subsequent embryonic development of SCNT.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 2","pages":"73-81"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9797395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Emerging Biological Functions of Exosomes from Dental Tissue-Derived Mesenchymal Stem Cells. 牙组织来源间充质干细胞外泌体的新生物学功能。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2022.0147

Shu Ma, Yidi Jiang, Yuyan Qian, Jing Du, Xiaoyan Yu, Shiyi Luo, Zhu Chen

{"title":"The Emerging Biological Functions of Exosomes from Dental Tissue-Derived Mesenchymal Stem Cells.","authors":"Shu Ma, Yidi Jiang, Yuyan Qian, Jing Du, Xiaoyan Yu, Shiyi Luo, Zhu Chen","doi":"10.1089/cell.2022.0147","DOIUrl":"https://doi.org/10.1089/cell.2022.0147","url":null,"abstract":"Exosomes are one kind of small-cell extracellular membranous vesicles that can regulate intercellular communication and give rise to mediating the biological behaviors of cells, involving in tissue formation, repair, the modulation of inflammation, and nerve regeneration. The abundant kinds of cells can secret exosomes, among them, mesenchymal stem cells (MSCs) are very perfect cells for mass production of exosomes. Dental tissue-derived mesenchymal stem cells (DT-MSCs), including dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, stem cells from human periodontal ligament (PDLSCs), gingiva-derived mesenchymal stem cells, dental follicle stem cells, tooth germ stem cells, and alveolar bone-derived mesenchymal stem cells, are now known as a potent tool in the area of cell regeneration and therapy, more importantly, DT-MSCs can also release numerous types of exosomes, participating in the biological functions of cells. Hence, we briefly depict the characteristics of exosomes, give a detailed description of the biological functions and clinical application in some respects of exosomes from DT-MSCs through systematically reviewing the latest evidence, and provide a rationale for their use as tools for potential application in tissue engineering.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 2","pages":"53-64"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9741250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1