Cellular reprogramming最新文献_第8页

Reprogramming Stars #12: At the Heart of In Vivo Reprogramming- An Interview with Dr. Li Qian. 重编程之星#12:体内重编程的核心——李茜博士访谈录

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 DOI: 10.1089/cell.2023.0056

Li Qian, Carlos-Filipe Pereira

引用次数: 0

MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells. 过表达 MicroRNA26a 可加快牙科干细胞的成骨细胞分化能力

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-18 DOI: 10.1089/cell.2023.0004

Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar

{"title":"MicroRNA26a Overexpression Hastens Osteoblast Differentiation Capacity in Dental Stem Cells.","authors":"Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar","doi":"10.1089/cell.2023.0004","DOIUrl":"10.1089/cell.2023.0004","url":null,"abstract":"Dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) are a source of mesenchymal stem cells with the potential to differentiate into several cell types. We initially isolated SHED cells and compared their osteogenic capacity with commercially available DPSCs. Both cells exhibited similar capacities of growth and osteogenic differentiation. A fourfold to sixfold increase in endogenous microRNA26a (miR26a) expression during osteogenic differentiation of preosteoblasts and a similar but attenuated increase (twofold to fourfold) in differentiating SHED was observed, suggesting a role in the process. We, therefore, overexpressed miR26a in SHED to determine if the osteogenic differentiation capacity can be potentiated in vitro. SHED with a threefold increase in miR26a expression showed increased growth rate when compared with parent cells. When exposed to an osteogenic differentiating promoting medium, the miR26a overexpressing cells showed 100-fold increases in the expression of bone marker genes such as type 1 collagen, alkaline phosphatase, and Runx2. The mineralization capacity of these cells was also increased 15-fold. As miR26a targets regulate several bone-specific genes, we evaluated the effect of miR26a overexpression on established targets. We found a moderate decrease in SMAD1 and a profound decrease in PTEN expression. miR26a could potentiate its effect on osteoblast differentiation by its ability to inhibit PTEN and increase the viability of cells and their numbers, a process essential in osteoblast differentiation. Our studies suggest that the upregulation of miR26a can increase bone formation and may serve as an important target to further investigate its potential in tissue engineering applications.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"109-120"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9705422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of All Human Transcription Factors on the Directed Differentiation of Pluripotent Stem Cells. 所有人转录因子对多能干细胞定向分化的影响。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2023.0007

Rodrigo L Dos Santos

引用次数: 0

Treatments of Porcine Nuclear Recipient Oocytes and Somatic Cell Nuclear Transfer-Generated Embryos with Various Reactive Oxygen Species Scavengers Lead to Improvements of Their Quality Parameters and Developmental Competences by Mitigating Oxidative Stress-Related Impacts. 用各种活性氧清除剂处理猪核受体卵母细胞和体细胞核移植胚胎，通过减轻氧化应激相关的影响，改善其质量参数和发育能力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2022.0145

Seung-Hwan Oh, Seung-Eun Lee, Dong-Hun Han, Jae-Wook Yoon, So-Hee Kim, Eun-Seo Lim, Han-Bi Lee, Eun-Young Kim, Se-Pill Park

{"title":"Treatments of Porcine Nuclear Recipient Oocytes and Somatic Cell Nuclear Transfer-Generated Embryos with Various Reactive Oxygen Species Scavengers Lead to Improvements of Their Quality Parameters and Developmental Competences by Mitigating Oxidative Stress-Related Impacts.","authors":"Seung-Hwan Oh, Seung-Eun Lee, Dong-Hun Han, Jae-Wook Yoon, So-Hee Kim, Eun-Seo Lim, Han-Bi Lee, Eun-Young Kim, Se-Pill Park","doi":"10.1089/cell.2022.0145","DOIUrl":"https://doi.org/10.1089/cell.2022.0145","url":null,"abstract":"This study investigated the antioxidant effects of β-cryptoxanthin (BCX), hesperetin (HES), and icariin (ICA), and their effects on in vitro maturation of porcine oocytes and subsequent embryonic development of somatic cell nuclear transfer (SCNT). Treatment with 1 μM BCX (BCX-1) increased the developmental rate of porcine oocytes more than treatment with 100 μM HES (HES-100) or 5 μM ICA (ICA-5). The glutathione level and mRNA expression of antioxidant genes (NFE2L2, SOD1, and SOD2) were more increased in the BCX-1 group than in the HES-100 and ICA-5 groups, while the reactive oxygen species level was more decreased. Moreover, BCX improved the developmental capacity and quality of SCNT embryos. The total cell number, apoptotic cell rate, and development-related gene expression were modulated in the BCX-1 group to enhance embryonic development of SCNT. These results show that the antioxidant effects of BCX enhance in vitro maturation of porcine oocytes and subsequent embryonic development of SCNT.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 2","pages":"73-81"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9797395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Emerging Biological Functions of Exosomes from Dental Tissue-Derived Mesenchymal Stem Cells. 牙组织来源间充质干细胞外泌体的新生物学功能。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2022.0147

Shu Ma, Yidi Jiang, Yuyan Qian, Jing Du, Xiaoyan Yu, Shiyi Luo, Zhu Chen

{"title":"The Emerging Biological Functions of Exosomes from Dental Tissue-Derived Mesenchymal Stem Cells.","authors":"Shu Ma, Yidi Jiang, Yuyan Qian, Jing Du, Xiaoyan Yu, Shiyi Luo, Zhu Chen","doi":"10.1089/cell.2022.0147","DOIUrl":"https://doi.org/10.1089/cell.2022.0147","url":null,"abstract":"Exosomes are one kind of small-cell extracellular membranous vesicles that can regulate intercellular communication and give rise to mediating the biological behaviors of cells, involving in tissue formation, repair, the modulation of inflammation, and nerve regeneration. The abundant kinds of cells can secret exosomes, among them, mesenchymal stem cells (MSCs) are very perfect cells for mass production of exosomes. Dental tissue-derived mesenchymal stem cells (DT-MSCs), including dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, stem cells from human periodontal ligament (PDLSCs), gingiva-derived mesenchymal stem cells, dental follicle stem cells, tooth germ stem cells, and alveolar bone-derived mesenchymal stem cells, are now known as a potent tool in the area of cell regeneration and therapy, more importantly, DT-MSCs can also release numerous types of exosomes, participating in the biological functions of cells. Hence, we briefly depict the characteristics of exosomes, give a detailed description of the biological functions and clinical application in some respects of exosomes from DT-MSCs through systematically reviewing the latest evidence, and provide a rationale for their use as tools for potential application in tissue engineering.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 2","pages":"53-64"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9741250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reprogramming Stars #11: Teaming Up to Uncover the Epitranscriptomics of Reprogramming-An Interview with Dr. Miguel Fidalgo and Dr. Diana Guallar. 重编程之星》第11期：联手揭示重编程的外转录组学--专访米格尔-菲达尔戈博士和戴安娜-瓜拉尔博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2023.0024

Miguel Fidalgo, Diana Guallar, Carlos-Filipe Pereira

引用次数: 0

A Fundamental Research in In Vitro Spermatogonial Stem Cell Culturing: What Are Clump Cells? 精原干细胞体外培养的基础研究:什么是团块细胞?

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-04-01 DOI: 10.1089/cell.2022.0123

Kiana Sojoudi, Hossein Azizi, Thomas Skutella

{"title":"A Fundamental Research in In Vitro Spermatogonial Stem Cell Culturing: What Are Clump Cells?","authors":"Kiana Sojoudi, Hossein Azizi, Thomas Skutella","doi":"10.1089/cell.2022.0123","DOIUrl":"https://doi.org/10.1089/cell.2022.0123","url":null,"abstract":"Spermatogonial stem cells (SSCs) are a small group of testicular cells located in the basement membrane of seminiferous tubules and can balance self-renewal and differentiation during spermatogenesis. Our in vitro culture experiments of mouse SSCs indicated heterogeneity of cultured cells. Highly compact colonies were observed next to SSC colonies, which we call clump cells. We used immunocytochemical staining to identify SSCs and somatic cells with VASA and Vimentin antibodies. Subsequently, we compared mRNA expression levels of VASA, DAZL, PLZF, GFRA1, Lin28, Kit, Myc and Vimentin genes using Fluidigm real-time RT-polymerase chain reaction in clump cells, SSCs, and testicular stromal cells. To better understand the functions of selected genes, we created a protein-protein interaction network and performed an enrichment analysis using different databases. Based on the data collected, we state that clump cells do not express the molecular markers of SSCs, so we cannot consider them as SSCs; however, we claim that these cells are altered SSCs. The molecular mechanism of this conversion is still obscure. Therefore, this study can support the analysis of germ cell development both in vitro and in vivo. In addition, it can be effective in finding new and more efficient treatments for male infertility.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 2","pages":"65-72"},"PeriodicalIF":1.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9744526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reprogramming Stars #10: Modeling Cancer with Cellular Reprogramming-An Interview with Dr. Dung-Fang Lee. 重编程明星 #10：用细胞重编程模拟癌症--专访 Dung-Fang Lee 博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-02-01 Epub Date: 2023-01-31 DOI: 10.1089/cell.2023.29081.dfl

Dung-Fang Lee, Carlos-Filipe Pereira

引用次数: 0

Early Life Reprogramming-Based Treatment Promotes Longevity. 基于生命早期重编程的治疗促进长寿。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-02-01 Epub Date: 2022-12-30 DOI: 10.1089/cell.2022.0153

Patrizia Pessina, Bruno Di Stefano

引用次数: 0

The Wisdom in Teeth: Neuronal Differentiation of Dental Pulp Cells. 牙齿中的智慧:牙髓细胞的神经元分化。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-02-01 DOI: 10.1089/cell.2022.0102

Bendegúz Sramkó, Anna Földes, Kristóf Kádár, Gábor Varga, Ákos Zsembery, Karolina Pircs

{"title":"The Wisdom in Teeth: Neuronal Differentiation of Dental Pulp Cells.","authors":"Bendegúz Sramkó, Anna Földes, Kristóf Kádár, Gábor Varga, Ákos Zsembery, Karolina Pircs","doi":"10.1089/cell.2022.0102","DOIUrl":"https://doi.org/10.1089/cell.2022.0102","url":null,"abstract":"Mesenchymal stem/stromal cells (MSCs) are found in almost all postnatal organs. Under appropriate environmental cues, multipotency enables MSCs to serve as progenitors for several lineage-specific, differentiated cell types. In vitro expansion and differentiation of MSCs give the opportunity to obtain hardly available somatic cells, such as neurons. The neurogenic potential of MSCs makes them a promising, autologous source to restore damaged tissue and as such, they have received much attention in the field of regenerative medicine. Several stem cell pool candidates have been studied thus far, but only a few of them showed neurogenic differentiation potential. Due to their embryonic ontology, stem cells residing in the stroma of the dental pulp chamber are an exciting source for in vitro neural cell differentiation. In this study, we review the key properties of dental pulp stem cells (DPSCs), with a particular focus on their neurogenic potential. Moreover, we summarize the various presently available methods used for neural differentiation of human DPSCs also emphasizing the difficulties in reproducibly high production of such cells. We postulate that because DPSCs are stem cells with very close ontology to neurogenic lineages, they may serve as excellent targets for neuronal differentiation in vitro and even for direct reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 1","pages":"32-44"},"PeriodicalIF":1.6,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9963504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9130559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4