Cellular reprogramming最新文献_第7页

The Ethics of Cellular Reprogramming. 细胞重新编程的伦理学。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-22 DOI: 10.1089/cell.2023.0091

Anna Smajdor, Adrian Villalba

{"title":"The Ethics of Cellular Reprogramming.","authors":"Anna Smajdor, Adrian Villalba","doi":"10.1089/cell.2023.0091","DOIUrl":"10.1089/cell.2023.0091","url":null,"abstract":"Louise Brown's birth in 1978 heralded a new era not just in reproductive technology, but in the relationship between science, cells, and society. For the first time, human embryos could be created, selected, studied, manipulated, frozen, altered, or destroyed, outside the human body. But with this possibility came a plethora of ethical questions. Is it acceptable to destroy a human embryo for the purpose of research? Or to create an embryo with the specific purpose of destroying it for research? In an attempt to construct ethical and legal frameworks for the new era of cellular reprogramming, legislators and ethicists have tried to distinguish between different kinds of biological entity. We treat cells differently depending on whether they are human or animal, somatic cells or gametes, and on whether they are embryos or not. But this approach to the ethics of cellular reprogramming is doomed to failure for the simple reason that cellular reprogramming in itself destroys the distinctions that the law requires to function. In this article, we explore the historical trajectory of cellular reprogramming and its relationship with ethics and society. We suggest that the early hype of embryo research has not obviously fulfilled expectations, but since new avenues of research are continuously opening, it is hard to say definitely that these promises have been broken. We explore the forthcoming challenges posed by the creation of DNA from scratch in the laboratory, and the implications of this for understandings of identity, privacy, and reproduction. We conclude that while ethics used to seek answers in biological facts, this is no longer possible, and a new approach is required.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"190-194"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41108115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons. 人类诱导的多能干细胞衍生的周细胞作为可扩展和可编辑的来源，研究将谱系重新编程为诱导的神经元。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-06-27 DOI: 10.1089/cell.2023.0008

Radhika Menon, Linda Petrucci, Benjamin Lohrer, Jingzhong Zhang, Markus Schulze, Christian Schichor, Beate Winner, Jürgen Winkler, Markus J Riemenschneider, Ralf Kühn, Sven Falk, Marisa Karow

{"title":"Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons.","authors":"Radhika Menon, Linda Petrucci, Benjamin Lohrer, Jingzhong Zhang, Markus Schulze, Christian Schichor, Beate Winner, Jürgen Winkler, Markus J Riemenschneider, Ralf Kühn, Sven Falk, Marisa Karow","doi":"10.1089/cell.2023.0008","DOIUrl":"10.1089/cell.2023.0008","url":null,"abstract":"Studying human somatic cell-to-neuron conversion using primary brain-derived cells as starting cell source is hampered by limitations and variations in human biopsy material. Thus, delineating the molecular variables that allow changing the identity of somatic cells, permit adoption of neuronal phenotypes, and foster maturation of induced neurons (iNs) is challenging. Based on our previous results that pericytes derived from the adult human cerebral cortex can be directly converted into iNs (Karow et al., 2018; Karow et al., 2012), we here introduce human induced pluripotent stem cell (hiPSC)-derived pericytes (hiPSC-pericytes) as a versatile and more uniform tool to study the pericyte-to-neuron conversion process. This strategy enables us to derive scalable cell numbers and allows for engineering of the starting cell population such as introducing reporter tools before differentiation into hiPSC-pericytes and subsequent iN conversion. Harvesting the potential of this approach, we established hiPSC-derived human-human neuronal cocultures that not only allow for independent manipulation of each coculture partner but also resulted in morphologically more mature iNs. In summary, we exploit hiPSC-based methods to facilitate the analysis of human somatic cell-to-neuron conversion.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"212-223"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9687149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopic Analysis of Cell Fate Alteration Induced by Cell Fusion. 细胞融合诱导的细胞命运改变的显微镜分析。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 DOI: 10.1089/cell.2023.0073

Taisei Kumazaki, Chinatsu Yonekawa, Tomomi Tsubouchi

{"title":"Microscopic Analysis of Cell Fate Alteration Induced by Cell Fusion.","authors":"Taisei Kumazaki, Chinatsu Yonekawa, Tomomi Tsubouchi","doi":"10.1089/cell.2023.0073","DOIUrl":"10.1089/cell.2023.0073","url":null,"abstract":"In mammals, differentiated cells generally do not de-differentiate nor undergo cell fate alterations. However, they can be experimentally guided toward a different lineage. Cell fusion involving two different cell types has long been used to study this process, as this method induces cell fate alterations within hours to days in a subpopulation of fused cells, as evidenced by changes in gene-expression profiles. Despite the robustness of this system, its use has been restricted by low fusion rates and difficulty in eliminating unfused populations, thereby compromising resolution. In this study, we address these limitations by isolating fused cells using antibody-conjugated beads. This approach enables the microscopic tracking of fused cells starting as early as 5 hours after fusion. By taking advantage of species-specific FISH probes, we show that a small population of fused cells resulting from the fusion of mouse ES and human B cells, expresses OCT4 from human nuclei at levels comparable to human induced pluripotent stem cells (iPSCs) as early as 25 hours after fusion. We also show that this response can vary depending on the fusion partner. Our study broadens the usage of the cell fusion system for comprehending the mechanisms underlying cell fate alterations. These findings hold promise for diverse fields, including regenerative medicine and cancer.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 5","pages":"251-259"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #13: Establishing Connections with Cellular Reprogramming-An Interview with Dr. Daniella Rylander Ottosson. 重编程明星 #13：与细胞重编程建立联系--专访 Daniella Rylander Ottosson 博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0078

Daniella Rylander Ottosson, Carlos-Filipe Pereira

引用次数: 0

Unleashing Ascl1: Exploring Cross-Lineage Potential in Reprogramming and Regenerative Frontiers. 释放Ascl1:探索重编程和再生前沿的跨谱系潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0080

Camila Vazquez Echegaray

引用次数: 0

Transcriptomic Heterogeneity of Human Mesenchymal Stem Cells Derived from Bone Marrow, Dental Pulp, Adipose Tissue, and Umbilical Cord. 来自骨髓、牙髓、脂肪组织和脐带的人间充质干细胞转录组异质性。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0019

Xiaoxiao Zhu, Xinchen Xu, Mengyuan Shen, Yingying Wang, Tao Zheng, Huitao Li, Xing Wang, Jian Meng

{"title":"Transcriptomic Heterogeneity of Human Mesenchymal Stem Cells Derived from Bone Marrow, Dental Pulp, Adipose Tissue, and Umbilical Cord.","authors":"Xiaoxiao Zhu, Xinchen Xu, Mengyuan Shen, Yingying Wang, Tao Zheng, Huitao Li, Xing Wang, Jian Meng","doi":"10.1089/cell.2023.0019","DOIUrl":"https://doi.org/10.1089/cell.2023.0019","url":null,"abstract":"Compared with mesenchymal stem cells (MSCs) obtained from other tissue sources, those derived from umbilical cord (UC) tissue exhibit numerous advantages and vast potential for therapeutic applications. However, MSCs from different tissue sources are heterogeneous, and therefore, the therapeutic efficacy of UC-derived MSCs as a replacement for other tissue-derived MSCs needs to be studied. To better understand the distinctions between UC-derived MSCs and MSCs derived from other tissues, we conducted a transcriptome analysis of MSCs obtained from UC and three other tissues. Correlation analysis revealed the strongest correlation between UC-MSCs (UC-MSCs) and bone marrow-MSCs (BM-MSCs). Compared with UC-MSCs, the lower differentially expressed genes of BM-MSCs, dental pulp-MSCs (DP-MSCs), and adipose tissue-MSCs (AP-MSCs) were predominantly enriched in actin-related terms, while higher differentially expressed genes were predominantly enriched in immunological processes. We also analyzed the distribution of 34 frequently or highly expressed cell characterization molecules in BM-MSCs, DP-MSCs, AP-MSCs, and UC-MSCs. CD200 (FPKM >10) was only detected in UC-MSCs, while CD106 was detected in AD-MSCs and DP-MSCs (FPKM >10). The reliability of transcriptomic data analysis was verified by quantitative real-time PCR. Finally, we recommend the use of CD200, CD106, and other similar markers with unstable expression as benchmark molecules to monitor the proliferation and differentiation potential of MSCs. This study provides comprehensive insights into the heterogeneity between UC-MSCs and MSCs derived from other tissues, which can guide the therapeutic application of UC-MSCs.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"162-170"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10489381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cell Reprogramming Techniques: Contributions to Cancer Therapy. 细胞重编程技术:对癌症治疗的贡献。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0011

Tongtong Guo, Qi Wei

{"title":"Cell Reprogramming Techniques: Contributions to Cancer Therapy.","authors":"Tongtong Guo, Qi Wei","doi":"10.1089/cell.2023.0011","DOIUrl":"https://doi.org/10.1089/cell.2023.0011","url":null,"abstract":"The reprogramming of terminally differentiated cells over the past few years has become important for induced pluripotent stem cells (iPSCs) in the field of regenerative medicine and disease drug modeling. At the same time, iPSCs have also played an important role in human cancer research. iPSCs derived from cancer patients can be used to simulate the early progression of cancer, for drug testing, and to study the molecular mechanism of cancer occurrence. In recent years, with the application of cellular immunotherapy in cancer therapy, patient-derived iPSC-induced immune cells (T, natural killer, and macrophage cells) solve the problem of immune rejection and have higher immunogenicity, which greatly improves the therapeutic efficiency of immune cell therapy. With the continuous progress of cancer differentiation therapy, iPSC technology can reprogram cancer cells to a more primitive pluripotent undifferentiated state, and successfully reverse cancer cells to a benign phenotype by changing the epigenetic inheritance of cancer cells. This article reviews the recent progress of cell reprogramming technology in human cancer research, focuses on the application of reprogramming technology in cancer immunotherapy and the problems solved, and summarizes the malignant phenotype changes of cancer cells in the process of reprogramming and subsequent differentiation.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"142-153"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10126026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for Cellular Reprogramming. 罗莎琳德·富兰克林协会自豪地宣布了2022年细胞重编程奖获得者。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.29097.rfs2022

Cristiana F Pires

引用次数: 0

A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation. 一种分离和冷冻保存人脂肪干细胞的新方法

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0017

Young-Cheol Lim, Jung-Il Jung, In-Kee Hong

{"title":"A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation.","authors":"Young-Cheol Lim, Jung-Il Jung, In-Kee Hong","doi":"10.1089/cell.2023.0017","DOIUrl":"https://doi.org/10.1089/cell.2023.0017","url":null,"abstract":"Adipose-derived stem cells (ADSCs) are isolated from abundant adipose tissue and have the capacity to differentiate into multiple cell lineages. ADSCs have raised big interest in therapeutic applications in regenerative medicine and demonstrated to fulfill the criteria for a successful cell therapy. There are several methods for isolation of ADSCs from adipose tissue and cryopreservation of ADSCs. Here, novel methods for the isolation and cryopreservation of ADSCs are presented and focused. Microscopic pieces of adipose tissue were placed on transwell inserts, and the ADSCs were induced to migrate to the lower wells for 1 week. We compared the properties of our ADSCs with those isolated by enzymatic digestion and enzyme-free method of culture plate, and our ADSCs were found to be more stable and healthier. In addition, we proposed a novel cryoprotectant solution (FNCP) containing pectin and L-alanine, which was compared with standard cryoprotectant solution. Overall, our methods proved more useful for ADSCs isolation than other methods and did not require consideration of \"minimal manipulation\" by the U.S. Food and Drug Administration (FDA). Furthermore, our FNCP did not contain dimethyl sulfoxide and fetal bovine serum, therefore stable storage is possible in xeno-free and animal-free cryopreservation solutions.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"171-179"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10138576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Transdifferentiation: A Challenging Strategy with Great Potential. 细胞转分化:一个具有巨大潜力的挑战策略。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0015

Fuping Wang, Runting Li, Limeng Zhang, Xiaoning Nie, Linqing Wang, Longxin Chen

引用次数: 0