Cellular reprogramming最新文献_第7页

Molecular Prospective on Malignant Transformation of Mesenchymal Stem Cells: An Issue in Cell Therapy. 间充质干细胞恶性转化的分子前瞻：细胞疗法中的一个问题。

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-06-01 DOI: 10.1089/cell.2024.0026

Maryam Kaviani, Saeede Soleimanian, Somayeh Keshtkar, Negar Azarpira, Zahra Asvar, Sara Pakbaz

{"title":"Molecular Prospective on Malignant Transformation of Mesenchymal Stem Cells: An Issue in Cell Therapy.","authors":"Maryam Kaviani, Saeede Soleimanian, Somayeh Keshtkar, Negar Azarpira, Zahra Asvar, Sara Pakbaz","doi":"10.1089/cell.2024.0026","DOIUrl":"10.1089/cell.2024.0026","url":null,"abstract":"Mesenchymal stem cell (MSCs) therapy, as a rapidly developing area of medicine, holds great promise for the treatment of a variety of medical conditions. MSCs are multipotent stem cells that can be isolated from various tissues and could self-renew and differentiate. They secrete cytokines and trophic factors that create a regenerative microenvironment and have immunomodulatory properties. Although clinical trials have been conducted with MSCs in various diseases, concerns regarding the possibility of malignant transformation of these cells have been raised. The studies showed a higher rate of hematological malignancy and carcinogenesis in experimental models after MSC transplantation. The mechanisms underlying malignant transformation of MSCs are complex and not fully understood, but they are believed to involve the presence of special signaling molecules and alterations in cell behavior regulation pathways. Possible pathways that lead to MSCs' oncogenic transformation occur through two mechanisms: spontaneous and stimulated malignant transformation, including cell fusion, fusion proteins, and the tumor microenvironment. MSC-based therapies have the potential to revolutionize medicine, and addressing the issue of malignancy is crucial to ensure their safety and efficacy. Therefore, the purpose of the present review is to summarize the potential mechanisms of the malignant transformation of MSCs. [Figure: see text].","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 3","pages":"96-106"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Defined Induced Pluripotent Stem Cell Lines Mimic Donor Red Blood Cell Antigen Profiles for Therapeutic and Diagnostic Use. 高度定义的诱导多能干细胞系模拟供体红细胞抗原谱，用于治疗和诊断。

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-06-01 DOI: 10.1089/cell.2024.0018

Lucas Ferioli Catelli, Péricles Natan Mendes da Costa, Felipe Augusto Rós, Evandra Strazza Rodrigues, Fernanda Ferreira Ursoli, Flávia Leite Souza Santos, Mayra Dorigan, Lílian Maria de Castilho, Dimas Tadeu Covas, Simone Kashima

{"title":"Highly Defined Induced Pluripotent Stem Cell Lines Mimic Donor Red Blood Cell Antigen Profiles for Therapeutic and Diagnostic Use.","authors":"Lucas Ferioli Catelli, Péricles Natan Mendes da Costa, Felipe Augusto Rós, Evandra Strazza Rodrigues, Fernanda Ferreira Ursoli, Flávia Leite Souza Santos, Mayra Dorigan, Lílian Maria de Castilho, Dimas Tadeu Covas, Simone Kashima","doi":"10.1089/cell.2024.0018","DOIUrl":"10.1089/cell.2024.0018","url":null,"abstract":"Our group generated two induced pluripotent stem cell (iPSC) lines for in vitro red blood cell (RBC) production from blood donors with extensively known erythrocyte antigen profiles. One line was intended to give rise to RBCs for transfusions in patients with sickle cell disease (SCD), while the other was developed to create RBC panel reagents. Two blood donors were selected based on their RBC phenotypes, further complemented by high-throughput DNA array analysis to obtain a more comprehensive erythrocyte antigen profile. Enriched erythroblast populations from the donors' peripheral blood mononuclear cells were reprogrammed into iPSCs using nonintegrative plasmid vectors. The iPSC lines were characterized and subsequently subjected to hematopoietic differentiation. iPSC PB02 and iPSC PB12 demonstrated in vitro and in vivo iPSC features and retained the genotype of each blood donor's RBC antigen profile. Colony-forming cell assays confirmed that iPSC PB02 and iPSC PB12 generated hematopoietic progenitors. These two iPSC lines were generated with defined erythrocyte antigen profiles, self-renewal capacity, and hematopoietic differentiation potential. With improvements in hematopoietic differentiation, these cells could potentially be more efficiently differentiated into RBCs in the future. They could serve as a complementary approach for obtaining donor-independent RBCs and addressing specific demands for blood transfusions.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 3","pages":"107-115"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalizing the Aging Immune System Through Selective Stem Cell Targeting. 通过选择性干细胞靶向激活老化的免疫系统

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-06-01 DOI: 10.1089/cell.2024.0029

Anna Konturek-Ciesla, David Bryder

引用次数: 0

Making Human Hematopoietic Stem Cells Without Transgenes. 制作不含转基因的人类造血干细胞。

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-04-01 Epub Date: 2024-03-26 DOI: 10.1089/cell.2024.0020

Luis G Palma, Anna Bigas

引用次数: 0

Acknowledgment of Reviewers 2023. 鸣谢 2023 年审稿人。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2024-02-01 Epub Date: 2023-12-22 DOI: 10.1089/cell.2024.29105.ack

引用次数: 0

Induction of Transient Morula-Like Cells in Mice Through STAT3 Activation. 通过 STAT3 激活诱导小鼠瞬时类木耳细胞

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-02-01 Epub Date: 2024-01-31 DOI: 10.1089/cell.2023.0116

Celia Fernandez-Rial, Miguel Fidalgo

引用次数: 0

Partial Reprogramming as a Method for Regenerating Neural Tissues in Aged Organisms. 部分重编程作为老化生物体神经组织再生的一种方法

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-02-01 DOI: 10.1089/cell.2023.0123

Ali Saber Sichani, Somayeh Khoddam, Shayan Shakeri, Zahra Tavakkoli, Arad Ranji Jafroodi, Reza Dabbaghipour, Mohsen Sisakht, Jafar Fallahi

{"title":"Partial Reprogramming as a Method for Regenerating Neural Tissues in Aged Organisms.","authors":"Ali Saber Sichani, Somayeh Khoddam, Shayan Shakeri, Zahra Tavakkoli, Arad Ranji Jafroodi, Reza Dabbaghipour, Mohsen Sisakht, Jafar Fallahi","doi":"10.1089/cell.2023.0123","DOIUrl":"10.1089/cell.2023.0123","url":null,"abstract":"Aging causes numerous age-related diseases, leading the human species to death. Nevertheless, rejuvenating strategies based on cell epigenetic modifications are a possible approach to counteract disease progression while getting old. Cell reprogramming of adult somatic cells toward pluripotency ought to be a promising tool for age-related diseases. However, researchers do not have control over this process as cells lose their fate, and cause potential cancerous cells or unexpected cell phenotypes. Direct and partial reprogramming were introduced in recent years with distinctive applications. Although direct reprogramming makes cells lose their identity, it has various applications in regeneration medicine. Temporary and regulated in vivo overexpression of Yamanaka factors has been shown in several experimental contexts to be achievable and is used to rejuvenate mice models. This regeneration can be accomplished by altering the epigenetic adult cell signature to the signature of a younger cell. The greatest advantage of partial reprogramming is that this method does not allow cells to lose their identity when they are resetting their epigenetic clock. It is a regimen of short-term Oct3/4, Sox2, Klf4, and c-Myc expression in vivo that prevents full reprogramming to the pluripotent state and avoids both tumorigenesis and the presence of unwanted undifferentiated cells. We know that many neurological age-related diseases, such as Alzheimer's disease, stroke, dementia, and Parkinson's disease, are the main cause of death in the last decades of life. Therefore, scientists have a special tendency regarding neuroregeneration methods to increase human life expectancy.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 1","pages":"10-23"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming Stars #14: Fast-Forwarding Cellular Reprogramming- An Interview with Dr. Mark Kotter. 重编程之星 #14：快速推进细胞重编程--马克-科特博士访谈。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2024-02-01 DOI: 10.1089/cell.2024.29111.mk

Mark R Kotter, Carlos-Filipe Pereira

引用次数: 0

Gene Therapy-Mediated Partial Reprogramming Extends Lifespan and Reverses Age-Related Changes in Aged Mice. 基因疗法介导的部分重编程延长了老年小鼠的寿命并逆转了与年龄有关的变化。

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-02-01 DOI: 10.1089/cell.2023.0072

Carolina Cano Macip, Rokib Hasan, Victoria Hoznek, Jihyun Kim, Yuancheng Ryan Lu, Louis E Metzger, Saumil Sethna, Noah Davidsohn

{"title":"Gene Therapy-Mediated Partial Reprogramming Extends Lifespan and Reverses Age-Related Changes in Aged Mice.","authors":"Carolina Cano Macip, Rokib Hasan, Victoria Hoznek, Jihyun Kim, Yuancheng Ryan Lu, Louis E Metzger, Saumil Sethna, Noah Davidsohn","doi":"10.1089/cell.2023.0072","DOIUrl":"10.1089/cell.2023.0072","url":null,"abstract":"Aging is a complex progression of changes best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ function. Although aging cannot currently be prevented, its impact on life- and healthspan in the elderly can potentially be minimized by interventions that aim to return these cellular processes to optimal function. Recent studies have demonstrated that partial reprogramming using the Yamanaka factors (or a subset; OCT4, SOX2, and KLF4; OSK) can reverse age-related changes in vitro and in vivo. However, it is still unknown whether the Yamanaka factors (or a subset) are capable of extending the lifespan of aged wild-type (WT) mice. In this study, we show that systemically delivered adeno-associated viruses, encoding an inducible OSK system, in 124-week-old male mice extend the median remaining lifespan by 109% over WT controls and enhance several health parameters. Importantly, we observed a significant improvement in frailty scores indicating that we were able to improve the healthspan along with increasing the lifespan. Furthermore, in human keratinocytes expressing exogenous OSK, we observed significant epigenetic markers of age reversal, suggesting a potential reregulation of genetic networks to a younger potentially healthier state. Together, these results may have important implications for the development of partial reprogramming interventions to reverse age-associated diseases in the elderly.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 1","pages":"24-32"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10909732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139912125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloned Foal Born from Postmortem-Obtained Ear Sample Refrigerated for 5 Days Before Fibroblast Isolation and Decontamination of the Infected Monolayer Culture. 在成纤维细胞分离和净化受感染的单层培养物之前，将从死后获得的耳部样本冷藏 5 天，克隆出小马。

IF 2.7 4区医学

Cellular reprogramming Pub Date : 2024-02-01 Epub Date: 2024-01-23 DOI: 10.1089/cell.2023.0076

Jenin V Cortez, Kylie Hardwicke, Christopher G Grupen, Muren Herrid, Zoltan Machaty, Gábor Vajta

{"title":"Cloned Foal Born from Postmortem-Obtained Ear Sample Refrigerated for 5 Days Before Fibroblast Isolation and Decontamination of the Infected Monolayer Culture.","authors":"Jenin V Cortez, Kylie Hardwicke, Christopher G Grupen, Muren Herrid, Zoltan Machaty, Gábor Vajta","doi":"10.1089/cell.2023.0076","DOIUrl":"10.1089/cell.2023.0076","url":null,"abstract":"A 6-year-old mare, a valuable polo horse, died of complications following postcolic surgery. To preserve its genetics, ear skin samples were collected immediately after death and stored in an equine embryo transfer medium at 4°C for 5 days. After trypsin digestion, monolayer fibroblast cultures were established, but signs of massive bacterial infection were found in all of them. As an ultimate attempt for rescue, rigorously and repeatedly washed cells were individually cultured in all wells of four 96-well dishes. New monolayers were established from the few wells without contamination and used for somatic cell nuclear transfer. Four of the six Day 7 blastocysts derived from 14 reconstructed zygotes were transferred in four naturally cycling mares on Day 5 after ovulation. The embryo transfers resulted in 2 pregnancies, one from a fresh and one from a vitrified blastocyst. The vitrified embryo transfer resulted in a healthy offspring, now 21 months old, genetically and phenotypically identical to the somatic cell donor animal.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"33-36"},"PeriodicalIF":2.7,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139542007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0