发布求助
文献互助 智能选刊 最新文献

Cellular reprogramming最新文献

筛选
英文 中文
Reprogramming of Expanded Cord Blood-Derived CD34+ Cells from Umbilical Cord-Mesenchymal Stromal Cell Co-Culture to Generate Human-Induced Pluripotent Stem Cells. 从脐带-间质基质细胞共培养中对扩增的脐带血CD34+细胞进行重编程，以生成人类诱导多能干细胞。
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0073
Fatin Fazrina Roslan, Yuexin Yu, Mengmeng Wang, Nurul Ain Nasim Mohd Yusof, Ghee Chien Ooi, Khong Lek Then, Kong Yong Then, Soon-Keng Cheong, Mohd Nor Azim Ab Patar, Jun Jie Tan
{"title":"Reprogramming of Expanded Cord Blood-Derived CD34<sup><b>+</b></sup> Cells from Umbilical Cord-Mesenchymal Stromal Cell Co-Culture to Generate Human-Induced Pluripotent Stem Cells.","authors":"Fatin Fazrina Roslan, Yuexin Yu, Mengmeng Wang, Nurul Ain Nasim Mohd Yusof, Ghee Chien Ooi, Khong Lek Then, Kong Yong Then, Soon-Keng Cheong, Mohd Nor Azim Ab Patar, Jun Jie Tan","doi":"10.1089/cell.2024.0073","DOIUrl":"10.1089/cell.2024.0073","url":null,"abstract":"<p><p>Cord blood (CB) is widely stored as a source of hematopoietic stem cells for potential future use, though its application for autologous purposes remains limited. Repurposing CB into human-induced pluripotent stem cells (hiPSCs) can broaden its utility beyond hematological conditions. This study investigated the effects of umbilical cord-mesenchymal stromal cell (UC-MSC) co-culture on CB CD34<sup>+</sup> cells and the characteristics of the resulting hiPSCs. CD34<sup>+</sup> cells were isolated, expanded in UC-MSC co-culture for 3 days, and reprogrammed into hiPSCs using episomal vectors. Results showed that UC-MSC co-culture significantly increased CD34<sup>+</sup> cell numbers (<i>p</i> < 0.0001, <i>n</i> = 6), with a reduced population doubling time of 25.1 ± 2.1 hours compared with the control (<i>p</i> < 0.0004, <i>n</i> = 6). The yield of CD34<sup>+</sup> cells was substantially higher in the UC-MSC co-culture group. The hiPSCs exhibited comparable reprogramming efficiency, pluripotency marker expression, trilineage differentiation potential, and genomic stability to CD34<sup>+</sup> cells expanded under standard culture conditions. These findings suggest that CD34<sup>+</sup> cells from CB, expanded in UC-MSC co-culture, can be reprogrammed into functional hiPSCs without compromising cell quality or genetic stability.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"164-176"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reprogramming Stars #19: Upgrading Cell Fate Conversions with Engineered Reprogramming Factors-An Interview with Dr. Ralf Jauch. 重编程之星 #19：利用工程重编程因子升级细胞命运转换--专访 Ralf Jauch 博士。
IF 1.2 4区 医学
Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI: 10.1089/cell.2024.0094
Ralf Jauch, Mariana Lopes, Carlos-Filipe Pereira
{"title":"Reprogramming Stars #19: Upgrading Cell Fate Conversions with Engineered Reprogramming Factors-An Interview with Dr. Ralf Jauch.","authors":"Ralf Jauch, Mariana Lopes, Carlos-Filipe Pereira","doi":"10.1089/cell.2024.0094","DOIUrl":"10.1089/cell.2024.0094","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"147-152"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reaching the Holy Grail: Making Hematopoietic Stem Cells in a Dish. 达到圣杯：在盘中制造造血干细胞。
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-12-01 Epub Date: 2024-11-26 DOI: 10.1089/cell.2024.0085
Riccardo Piussi, Andrea Ditadi
{"title":"Reaching the Holy Grail: Making Hematopoietic Stem Cells in a Dish.","authors":"Riccardo Piussi, Andrea Ditadi","doi":"10.1089/cell.2024.0085","DOIUrl":"10.1089/cell.2024.0085","url":null,"abstract":"<p><p>The successful generation of long-term engrafting hematopoietic stem cells (HSCs) from human-induced pluripotent stem cells (hiPSCs) has long been sought to revolutionize treatments for hematological disorders, eliminating reliance on donors and avoiding immune rejection, and thus has been seen as a major milestone in regenerative medicine. Previous studies, guided by developmental hematopoiesis, made progress in creating blood cells from hiPSCs, but challenges persisted in producing hematopoietic cells with functional properties of genuine HSCs capable of long-term engraftment. In their recent study, Ng and colleagues described an optimized differentiation protocol that manipulates key signaling pathways, including TGF-β, WNT, BMP, and retinoic acid in a stage-specific manner to generate HSCs with multilineage capacity. This strategy yielded hematopoietic cells capable of engrafting long term with high levels of human chimerism in recipient mice. This research provides a blueprint for future studies aiming for personalized HSC-based therapies for various blood disorders.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"153-155"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reprogramming Stars #18: Engineering Cell Fates and Preventing Disease by Repressing Unwanted Plasticity-An Interview with Dr. Moritz Mall. 重编程之星 #18：通过抑制不必要的可塑性来改造细胞命运和预防疾病--莫里茨-马尔博士访谈录。
IF 1.2 4区 医学
Cellular reprogramming Pub Date : 2024-10-01 DOI: 10.1089/cell.2024.36789.mor
Moritz Mall, Mariana Lopes, Carlos-Filipe Pereira
{"title":"Reprogramming Stars #18: Engineering Cell Fates and Preventing Disease by Repressing Unwanted Plasticity-An Interview with Dr. Moritz Mall.","authors":"Moritz Mall, Mariana Lopes, Carlos-Filipe Pereira","doi":"10.1089/cell.2024.36789.mor","DOIUrl":"https://doi.org/10.1089/cell.2024.36789.mor","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 5","pages":"125-131"},"PeriodicalIF":1.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A New Frontier in Tumor Eradication: Harnessing In Vivo Cellular Reprogramming for Durable Cancer Immunotherapy. 根除肿瘤的新前沿：利用体内细胞重编程实现持久的癌症免疫疗法。
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-10-01 Epub Date: 2024-10-10 DOI: 10.1089/cell.2024.0077
Constantinos Chronis
{"title":"A New Frontier in Tumor Eradication: Harnessing <i>In Vivo</i> Cellular Reprogramming for Durable Cancer Immunotherapy.","authors":"Constantinos Chronis","doi":"10.1089/cell.2024.0077","DOIUrl":"10.1089/cell.2024.0077","url":null,"abstract":"<p><p>Tumors evade immune detection by downregulating antigen presentation and hindering immune responses. Type 1 conventional dendritic cells (cDC1s) are vital in stimulating cytotoxic T cells against tumors. Ascic et al. are now demonstrating the <i>in situ</i> ability of PU.1, IRF8, and BATF3 (PIB) transcription factors to directly reprogram a plethora of tumors bypassing the suppressive effects of the tumor microenvironment, and leading to overall tumor regression while eliciting a systemic immune response that can protect from secondary tumor induction.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"132-134"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142459023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deciphering the Sertoli Cell Signaling Pathway with Protein-Protein Interaction, Single-Cell Sequencing, and Gene Ontology. 利用蛋白质-蛋白质相互作用、单细胞测序和基因本体解密Sertoli细胞信号通路
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-10-01 DOI: 10.1089/cell.2024.0059
Danial Hashemi Karoii, Gohar Javadzadeh, Hossein Azizi, Fadhil Farhood M Al-Joborae, Mehdi Amirian
{"title":"Deciphering the Sertoli Cell Signaling Pathway with Protein-Protein Interaction, Single-Cell Sequencing, and Gene Ontology.","authors":"Danial Hashemi Karoii, Gohar Javadzadeh, Hossein Azizi, Fadhil Farhood M Al-Joborae, Mehdi Amirian","doi":"10.1089/cell.2024.0059","DOIUrl":"10.1089/cell.2024.0059","url":null,"abstract":"<p><p>Spermatogenesis constitutes a complex and intricate cascade of differentiation, indispensable for the male reproductive competence. The intercellular communication conduits of Sertoli cells (SCs) are pivotal in orchestrating this cascade ensuring sustenance and development of germ cells. Single cells and bioinformatics recently demonstrated articles are used for the regulatory modalities through which SCs modulate spermatogenesis, specifically <i>via</i> androgen receptors (ARs), the transforming growth factor-beta/Smad axis, mitogen-activated protein kinases, cAMP/protein kinase A (PKA), phosphatidylinositol 4,5-bisphosphate 3-kinase (PI3k)/AKT serine threonine kinase (Akt), AMP-activated protein kinase, and AR pathways. Within this framework, homeostasis of gap junction dynamics, cryptic sites and the activities at tight junctions and adherens junctions, with the integrity of the testicular barrier, glucose assimilation, lactate distribution, being governed also along with SC maturation. Disruptions in activities or abnormal concentration in derangements in AR, cAMP/PKA, and PI3k/Akt pathways, and as well as the molecules that comprise them, would present male infertility.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 5","pages":"135-145"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rewinding the Tape to Identify Intrinsic Determinants of Reprogramming Potential. 倒带以确定重编程潜能的内在决定因素。
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-08-01 Epub Date: 2024-08-08 DOI: 10.1089/cell.2024.0035
Kate E Galloway
{"title":"Rewinding the Tape to Identify Intrinsic Determinants of Reprogramming Potential.","authors":"Kate E Galloway","doi":"10.1089/cell.2024.0035","DOIUrl":"10.1089/cell.2024.0035","url":null,"abstract":"<p><p>Via retrospective isolation of clones using Rewind, Jain et al. identified primed states of cells that reprogram to induced pluripotent stem cells. Examining clones, they find that cells retain memory of over several rounds of cell division. Moreover, they show that extrinsic factors change the number of primed cells, suggesting that there exist diverse paths of reprogramming and states of priming.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"117-119"},"PeriodicalIF":2.7,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-Scale Analyses Reveal Roadblocks to Monkey Cloning. 基因组规模分析揭示克隆猴的障碍
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-08-01 DOI: 10.1089/cell.2024.0048
Marcelo Tigre Moura
{"title":"Genome-Scale Analyses Reveal Roadblocks to Monkey Cloning.","authors":"Marcelo Tigre Moura","doi":"10.1089/cell.2024.0048","DOIUrl":"10.1089/cell.2024.0048","url":null,"abstract":"<p><p>Cloning by somatic cell nuclear transfer (SCNT) remained challenging for Rhesus monkeys, mostly due to its low efficiency and neonatal death. Genome-scale analyses revealed that monkey SCNT embryos displayed widespread DNA methylation and transcriptional alterations, thus including loss of genomic imprinting that correlated with placental dysfunction. The transfer of inner cell masses (ICM) from cloned blastocysts into ICM-depleted fertilized embryos rescued placental insufficiency and gave rise to a cloned Rhesus monkey that reached adulthood without noticeable abnormalities.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"120-123"},"PeriodicalIF":2.7,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reprogramming Stars #16: Reprogramming, from Cells to Embryos-An Interview with Dr. José Silva. 重编程之星 #16：重编程，从细胞到胚胎--专访何塞-席尔瓦博士。
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-06-01 DOI: 10.1089/cell.2024.26895.jcrs
José C R Silva, Carlos-Filipe Pereira
{"title":"Reprogramming Stars #16: Reprogramming, from Cells to Embryos-An Interview with Dr. José Silva.","authors":"José C R Silva, Carlos-Filipe Pereira","doi":"10.1089/cell.2024.26895.jcrs","DOIUrl":"10.1089/cell.2024.26895.jcrs","url":null,"abstract":"","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"26 3","pages":"85-90"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141449809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Impact of Senescent Cells on Limb Regeneration. 衰老细胞对肢体再生的影响
IF 2.7 4区 医学
Cellular reprogramming Pub Date : 2024-06-01 Epub Date: 2024-05-07 DOI: 10.1089/cell.2024.0021
Marlene J Oesterle, Nicholas D Leigh
{"title":"The Impact of Senescent Cells on Limb Regeneration.","authors":"Marlene J Oesterle, Nicholas D Leigh","doi":"10.1089/cell.2024.0021","DOIUrl":"10.1089/cell.2024.0021","url":null,"abstract":"<p><p>Cellular senescence is a state in which cells enter cell cycle arrest. However, senescent cells have the ability to secrete signaling molecules such as chemokines, cytokines, and growth factors. This secretory activity is an important feature of senescent cells, since the secreted factors impact the surrounding cellular microenvironment. Indeed, senescent cells and their secretome play a crucial role during limb development. However, whether the process of limb regeneration also relies on senescent cells remains unclear. Creation of a novel targeted depletion strategy that can eliminate senescent cells in the regenerating limb has now demonstrated an important role for senescent cells in limb regeneration. This role is linked to senescent cell-derived Wnt signaling. These findings reveal a previously unknown role for senescent cells during limb regeneration through Wnt signaling.</p>","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"91-92"},"PeriodicalIF":2.7,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书