Cellular reprogramming最新文献_第6页

Reprogramming Stars #13: Establishing Connections with Cellular Reprogramming-An Interview with Dr. Daniella Rylander Ottosson. 重编程明星 #13：与细胞重编程建立联系--专访 Daniella Rylander Ottosson 博士。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0078

Daniella Rylander Ottosson, Carlos-Filipe Pereira

引用次数: 0

Unleashing Ascl1: Exploring Cross-Lineage Potential in Reprogramming and Regenerative Frontiers. 释放Ascl1:探索重编程和再生前沿的跨谱系潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0080

Camila Vazquez Echegaray

引用次数: 0

Transcriptomic Heterogeneity of Human Mesenchymal Stem Cells Derived from Bone Marrow, Dental Pulp, Adipose Tissue, and Umbilical Cord. 来自骨髓、牙髓、脂肪组织和脐带的人间充质干细胞转录组异质性。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0019

Xiaoxiao Zhu, Xinchen Xu, Mengyuan Shen, Yingying Wang, Tao Zheng, Huitao Li, Xing Wang, Jian Meng

{"title":"Transcriptomic Heterogeneity of Human Mesenchymal Stem Cells Derived from Bone Marrow, Dental Pulp, Adipose Tissue, and Umbilical Cord.","authors":"Xiaoxiao Zhu, Xinchen Xu, Mengyuan Shen, Yingying Wang, Tao Zheng, Huitao Li, Xing Wang, Jian Meng","doi":"10.1089/cell.2023.0019","DOIUrl":"https://doi.org/10.1089/cell.2023.0019","url":null,"abstract":"Compared with mesenchymal stem cells (MSCs) obtained from other tissue sources, those derived from umbilical cord (UC) tissue exhibit numerous advantages and vast potential for therapeutic applications. However, MSCs from different tissue sources are heterogeneous, and therefore, the therapeutic efficacy of UC-derived MSCs as a replacement for other tissue-derived MSCs needs to be studied. To better understand the distinctions between UC-derived MSCs and MSCs derived from other tissues, we conducted a transcriptome analysis of MSCs obtained from UC and three other tissues. Correlation analysis revealed the strongest correlation between UC-MSCs (UC-MSCs) and bone marrow-MSCs (BM-MSCs). Compared with UC-MSCs, the lower differentially expressed genes of BM-MSCs, dental pulp-MSCs (DP-MSCs), and adipose tissue-MSCs (AP-MSCs) were predominantly enriched in actin-related terms, while higher differentially expressed genes were predominantly enriched in immunological processes. We also analyzed the distribution of 34 frequently or highly expressed cell characterization molecules in BM-MSCs, DP-MSCs, AP-MSCs, and UC-MSCs. CD200 (FPKM >10) was only detected in UC-MSCs, while CD106 was detected in AD-MSCs and DP-MSCs (FPKM >10). The reliability of transcriptomic data analysis was verified by quantitative real-time PCR. Finally, we recommend the use of CD200, CD106, and other similar markers with unstable expression as benchmark molecules to monitor the proliferation and differentiation potential of MSCs. This study provides comprehensive insights into the heterogeneity between UC-MSCs and MSCs derived from other tissues, which can guide the therapeutic application of UC-MSCs.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"162-170"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10489381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cell Reprogramming Techniques: Contributions to Cancer Therapy. 细胞重编程技术:对癌症治疗的贡献。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0011

Tongtong Guo, Qi Wei

{"title":"Cell Reprogramming Techniques: Contributions to Cancer Therapy.","authors":"Tongtong Guo, Qi Wei","doi":"10.1089/cell.2023.0011","DOIUrl":"https://doi.org/10.1089/cell.2023.0011","url":null,"abstract":"The reprogramming of terminally differentiated cells over the past few years has become important for induced pluripotent stem cells (iPSCs) in the field of regenerative medicine and disease drug modeling. At the same time, iPSCs have also played an important role in human cancer research. iPSCs derived from cancer patients can be used to simulate the early progression of cancer, for drug testing, and to study the molecular mechanism of cancer occurrence. In recent years, with the application of cellular immunotherapy in cancer therapy, patient-derived iPSC-induced immune cells (T, natural killer, and macrophage cells) solve the problem of immune rejection and have higher immunogenicity, which greatly improves the therapeutic efficiency of immune cell therapy. With the continuous progress of cancer differentiation therapy, iPSC technology can reprogram cancer cells to a more primitive pluripotent undifferentiated state, and successfully reverse cancer cells to a benign phenotype by changing the epigenetic inheritance of cancer cells. This article reviews the recent progress of cell reprogramming technology in human cancer research, focuses on the application of reprogramming technology in cancer immunotherapy and the problems solved, and summarizes the malignant phenotype changes of cancer cells in the process of reprogramming and subsequent differentiation.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"142-153"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10126026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for Cellular Reprogramming. 罗莎琳德·富兰克林协会自豪地宣布了2022年细胞重编程奖获得者。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.29097.rfs2022

Cristiana F Pires

引用次数: 0

A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation. 一种分离和冷冻保存人脂肪干细胞的新方法

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0017

Young-Cheol Lim, Jung-Il Jung, In-Kee Hong

{"title":"A Novel Method for Human Adipose-Derived Stem Cell Isolation and Cryopreservation.","authors":"Young-Cheol Lim, Jung-Il Jung, In-Kee Hong","doi":"10.1089/cell.2023.0017","DOIUrl":"https://doi.org/10.1089/cell.2023.0017","url":null,"abstract":"Adipose-derived stem cells (ADSCs) are isolated from abundant adipose tissue and have the capacity to differentiate into multiple cell lineages. ADSCs have raised big interest in therapeutic applications in regenerative medicine and demonstrated to fulfill the criteria for a successful cell therapy. There are several methods for isolation of ADSCs from adipose tissue and cryopreservation of ADSCs. Here, novel methods for the isolation and cryopreservation of ADSCs are presented and focused. Microscopic pieces of adipose tissue were placed on transwell inserts, and the ADSCs were induced to migrate to the lower wells for 1 week. We compared the properties of our ADSCs with those isolated by enzymatic digestion and enzyme-free method of culture plate, and our ADSCs were found to be more stable and healthier. In addition, we proposed a novel cryoprotectant solution (FNCP) containing pectin and L-alanine, which was compared with standard cryoprotectant solution. Overall, our methods proved more useful for ADSCs isolation than other methods and did not require consideration of \"minimal manipulation\" by the U.S. Food and Drug Administration (FDA). Furthermore, our FNCP did not contain dimethyl sulfoxide and fetal bovine serum, therefore stable storage is possible in xeno-free and animal-free cryopreservation solutions.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"171-179"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10138576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Transdifferentiation: A Challenging Strategy with Great Potential. 细胞转分化:一个具有巨大潜力的挑战策略。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0015

Fuping Wang, Runting Li, Limeng Zhang, Xiaoning Nie, Linqing Wang, Longxin Chen

引用次数: 0

A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models. 细胞年轻化的更安全途径:在早衰小鼠模型中通过CRISPR/dCas9内源性Oct4激活

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-08-01 DOI: 10.1089/cell.2023.0057

Di Hu, Enora Le Borgne, Rico Meinl

{"title":"A Safer Path to Cellular Rejuvenation: Endogenous Oct4 Activation via CRISPR/dCas9 in Progeria Mouse Models.","authors":"Di Hu, Enora Le Borgne, Rico Meinl","doi":"10.1089/cell.2023.0057","DOIUrl":"https://doi.org/10.1089/cell.2023.0057","url":null,"abstract":"A recent study in Aging Cell showed that transcriptional activation of endogenous Oct4 using the CRISPR/dCas9 activator system is sufficient for cellular rejuvenation and extending the lifespan of a progeria mouse model. Although transient expression of reprogramming factors Oct4, Sox2, Klf4, and c-Myc (OSKM) has been shown to ameliorate age-related phenotypes in vivo, oncogenic risk, for example, from c-Myc, has raised safety concerns for its use in therapeutics. The authors demonstrated that transient activation of endogenous Oct4 expression restored age-related epigenetic patterns, suppressed expression of mutant progerin, and reduced vascular pathological features associated with the disease. At the same time, the transient Oct4 overexpression resulted in lower incidence of cancer transformation compared with constituent OSKM overexpression. Successful activation of endogenous Oct4 by CRISPR/dCas9 paves the way for novel therapeutic approaches for the treatment of progeria and age-related diseases, with potential implications for the broader field of cellular reprogramming-based rejuvenation.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 4","pages":"136-138"},"PeriodicalIF":1.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Emerging Role of B1 SINE in Pluripotent Reprogramming. B1 SINE 在多能性重编程中的新作用。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-08 DOI: 10.1089/cell.2023.0037

Jere Weltner, Ras Trokovic

引用次数: 0

Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment. 肿瘤微环境中糖酵解代谢关键酶的功能

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-06-01 Epub Date: 2023-05-12 DOI: 10.1089/cell.2023.0010

Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen

{"title":"Functions of Key Enzymes of Glycolytic Metabolism in Tumor Microenvironment.","authors":"Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen","doi":"10.1089/cell.2023.0010","DOIUrl":"10.1089/cell.2023.0010","url":null,"abstract":"The tumor microenvironment (TME) plays a crucial role in tumor initiation, growth and metastasis. Metabolic enzymes involved in tumor glycolytic reprogramming, including hexokinase, pyruvate kinase, and lactate dehydrogenase, not only play key roles in tumorigenesis and maintaining tumor cell survival, but also take part in the modulation of the TME. Many studies have been devoted to the role of key glycolytic enzymes in the TME over the past decades. We summarize the studies on the role of glycolytic enzymes in the TME of these years and found that glycolytic enzymes remodel the TME primarily through regulating immune escape, angiogenesis, and affecting stromal cells and exosomes. Notably, abnormal tumor vascular system, peritumoral stromal cells, and tumor immunosuppressive microenvironment are important contributors to the failure of antitumor therapy. Therefore, we discuss the mechanisms of regulation by key glycolytic enzymes that may contribute to a promising biomarker for therapeutic intervention. We argue that targeting key glycolytic enzymes in combination with antiprogrammed cell death ligand 1 or antivascular endothelial growth factor could emerge as the more integrated and comprehensive antitumor treatment strategy.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 3","pages":"91-98"},"PeriodicalIF":1.6,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9704895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0