Cellular reprogramming最新文献_第6页

SIRT6 Knockdown in Buffalo Fetal Fibroblasts Exacerbates Premature Senescence Caused by DNA and Telomere Damage. 水牛胎儿成纤维细胞SIRT6基因敲除加剧DNA和端粒损伤引起的早衰。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 Epub Date: 2023-09-19 DOI: 10.1089/cell.2023.0048

Jingyuan Liang, Jiayu Cui, Juanru Cheng, Yu Pan, Ruimen Zhang, Sufang Yang, Lingxiu Zou

{"title":"SIRT6 Knockdown in Buffalo Fetal Fibroblasts Exacerbates Premature Senescence Caused by DNA and Telomere Damage.","authors":"Jingyuan Liang, Jiayu Cui, Juanru Cheng, Yu Pan, Ruimen Zhang, Sufang Yang, Lingxiu Zou","doi":"10.1089/cell.2023.0048","DOIUrl":"10.1089/cell.2023.0048","url":null,"abstract":"As a gene with antiaging functions, sirtuin6 (SIRT6) belonging to the sirtuin family plays a vital role in DNA repair, telomerase function, and cellular senescence, as well as maintains epigenomic stability and promotes longevity. However, its role in cell senescence in large animals, such as buffaloes, remains unknown. Fibroblasts are commonly used for somatic reprogramming, and their physiological characteristics affect the efficiency of this process. We aimed to elucidate the role of SIRT6 in cellular senescence and proliferation and analyze its effect on the biological function of buffalo fibroblasts to help improve the efficiency of buffalo somatic cell reprogramming. The expression of SIRT6 and related DNA damage was measured in buffalo fibroblasts obtained at different developmental stages (in the fetus and at 3 and 10 years of age), and the effect of SIRT6 knockdown on the senescence of buffalo fetal fibroblast was investigated. An inverse relationship was observed between SIRT6 expression and senescence in buffalo fibroblasts obtained from animals of various ages. This was accompanied by decreased cell growth, viability, and increased DNA damage. Short hairpin RNA-mediated SIRT6 knockdown accelerated the senescence of buffalo fetal fibroblasts. It blocked the cell cycle during in vitro cell culture, which further enhanced DNA damage, particularly with respect to the telomeres. Collectively, our findings suggest that SIRT6 expression was closely associated with buffalo senescence in fibroblasts. These findings serve as a foundation to better understand the cellular functions of SIRT6 and also aid in selecting donor cells for buffalo somatic cell reprogramming.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"277-287"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41112255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-Intensity Nanosecond Pulsed Electric Field Accelerates Osteogenic Transformation of Human Dermal Fibroblasts by Enhancing Cell Pluripotency. 低强度纳秒脉冲电场通过增强细胞多能性促进人真皮成纤维细胞成骨转化。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1089/cell.2023.0059

Jingtian Lai, Zewei Wang, Haiying Zhou, Pengfei Li, Hui Lu, Tian Tu

{"title":"Low-Intensity Nanosecond Pulsed Electric Field Accelerates Osteogenic Transformation of Human Dermal Fibroblasts by Enhancing Cell Pluripotency.","authors":"Jingtian Lai, Zewei Wang, Haiying Zhou, Pengfei Li, Hui Lu, Tian Tu","doi":"10.1089/cell.2023.0059","DOIUrl":"10.1089/cell.2023.0059","url":null,"abstract":"Autologous human fibroblasts have the potential to differentiate into the osteogenic lineage under specific conditions and can be utilized for bone regeneration. However, their efficiency is currently unsatisfactory. Recently, low-intensity nanosecond pulsed electric field (nsPEF) stimulation has been demonstrated to enhance cell pluripotency by activating epigenetic regulatory pathways. In this study, human dermal fibroblasts were exposed to different intensities of nsPEF to assess whether these exposures resulted in changes in proliferation rate, calcium salt deposition, and expression of differentiation-related markers in different experimental groups. The results showed a significant increase in cell proliferation, pluripotency, bone marker expression, and osteogenic differentiation efficiency when stimulating cells with 5 kV/cm of nsPEF. However, cell proliferation and differentiation significantly decreased at 25 kV/cm. Additionally, the proliferation and efficiency of osteogenic differentiation were reduced when the nsPEF intensity was increased to 50 kV/cm. Treatment with a 5 kV/cm of nsPEF led to increased and concentrated expression of Yes-Associated Protein (YAP) in the nucleus. These observations suggest that human dermal fibroblasts possess a heightened potential to differentiate into osteogenic cells when activated with nsPEF at 5 kV/cm. Consequently, the nsPEF strengthening strategy shows promise for fibroblast-based tissue-engineered bone repair research.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"300-309"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138443991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allogeneic Mesenchymal Stem Cells After In Vivo Transplantation: A Review. 同种异体间充质干细胞体内移植研究进展

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 Epub Date: 2023-11-16 DOI: 10.1089/cell.2023.0084

Derek B Asserson

引用次数: 0

Gene Regulatory Networks: Improving Inferences with Transfer Learning. 基因调控网络：利用迁移学习改进推断。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 DOI: 10.1089/cell.2023.0095

Marcelo Tigre Moura

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Pioneer of Cloning and Inspirational Figure for Cellular Reprogramming Scientists Sir Ian Wilmut (July 7, 1944-September 10, 2023). Ian Wilmut爵士（1944年7月7日至2023年9月10日）是细胞重新编程科学家克隆和启发性图形的先驱。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 DOI: 10.1089/cell.2023.29102.mem

Carlos-Filipe Pereira

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Hepatitis B Virus x Protein Increases Cellular OCT3/4 and MYC and Facilitates Cellular Reprogramming. 乙型肝炎病毒x蛋白增加细胞OCT3/4和MYC，促进细胞重新编程。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-26 DOI: 10.1089/cell.2023.0055

Madhusudana Girija Sanal, Sarita Gupta, Rahul Saha, Nisha Vats, Shiv Kumar Sarin

{"title":"Hepatitis B Virus x Protein Increases Cellular OCT3/4 and MYC and Facilitates Cellular Reprogramming.","authors":"Madhusudana Girija Sanal, Sarita Gupta, Rahul Saha, Nisha Vats, Shiv Kumar Sarin","doi":"10.1089/cell.2023.0055","DOIUrl":"10.1089/cell.2023.0055","url":null,"abstract":"Hepatitis B virus x (HBx) is a multifunctional protein coded by the Hepatitis B virus that is involved in various cellular processes such as proliferation, cell survival/apoptosis, and histone methylation. HBx was reported to be associated with liver \"cancer stem cells.\" The stemness inducing properties of HBx could also facilitate the generation of pluripotent stem cells from somatic cells. It is well established that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using a cocktail of transcription factors called Yamanaka's factors (YFs) (OCT4, SOX2, KLF4, and MYC). The reprogramming process proceeds step-by-step with reprogramming factor chromatin interactions, transcription, and chromatin states changing during transitions. HBx is a \"broad spectrum trans-activator\" and therefore could facilitate these transitions. We electroporated low passage and high passage (difficult to reprogram) fibroblasts using YFs with and without HBx and evaluated the reprogramming efficiency. We also investigated the tri-lineage and terminal differentiation potential of iPSC derived using HBx. We found that the addition of HBx to YF improves iPSC derivation, and it increases the efficiency of iPSC generation from \"difficult or hard-to-reprogram samples\" such as high passage/senescent fibroblasts. Further, we show that HBx can substitute the key transcription factor MYC in the YF cocktail to generate iPSC. The cellular levels of OCT3/4 and MYC were increased in HBx expressing cells. Our results have practical value in improving the efficiency of pluripotent stem cell derivation from \"difficult to reprogram\" somatic cells, in addition to providing some insights into the mechanisms of liver carcinogenesis in chronic hepatitis B. To conclude, HBx improves the reprogramming efficiency of YFs. HBx increases the cellular levels of OCT3/4 and MYC.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"224-237"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41119728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor T Cells: The Intersection of Stem Cells and Immunotherapy. 诱导的多能干细胞衍生的嵌合抗原受体T细胞：干细胞与免疫治疗的交叉。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-29 DOI: 10.1089/cell.2023.0041

Mohammad Reza Lahimchi, Faezeh Maroufi, Amirhosein Maali

{"title":"Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor T Cells: The Intersection of Stem Cells and Immunotherapy.","authors":"Mohammad Reza Lahimchi, Faezeh Maroufi, Amirhosein Maali","doi":"10.1089/cell.2023.0041","DOIUrl":"10.1089/cell.2023.0041","url":null,"abstract":"Chimeric antigen receptor (CAR) T cell therapy is a promising cell-based immunotherapy applicable to various cancers. High cost of production, immune rejection, heterogeneity of cell product, limited cell source, limited expandability, and relatively long production time have created the need to achieve a universal allogeneic CAR-T cell product for \"off-the-shelf\" application. Since the innovation of induced pluripotent stem cells (iPSCs) by Yamanaka et al., extensive efforts have been made to prepare an unlimited cell source for regenerative medicine, that is, immunotherapy. In the autologous grafting approach, iPSCs prepare the desired cell source for generating autologous CAR-T cells through more accessible and available sources. In addition, generating iPSC-derived CAR-T cells is a promising approach to achieving a suitable source for producing an allogeneic CAR-T cell product. In brief, the first step is reprogramming somatic cells (accessible from peripheral blood, skin, etc.) to iPSCs. In the next step, CAR expression and T cell lineage differentiation should be applied in different arrangements. In addition, in an allogeneic manner, human leukocyte antigen/T cell receptor (TCR) deficiency should be applied in iPSC colonies. The allogeneic iPSC-derived CAR-T cell experiments showed that simultaneous performance of HLA/TCR deficiency, CAR expression, and T cell lineage differentiation could bring the production to the highest efficacy in generating allogeneic iPSC-derived CAR-T cells.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"195-211"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient A·T-to-C·G Base Editing via Adenine Transversion Editors. 通过Adenine Transversion Editors进行高效的A·T到C·G基础编辑。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-19 DOI: 10.1089/cell.2023.0094

Muhammad Arslan Mahmood

引用次数: 0

Reprogramming Cell Identity: Past Lessons, Challenges, and Future Directions. 重新编程细胞身份：过去的教训，挑战和未来的方向。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 DOI: 10.1089/cell.2023.0100

José C R Silva

引用次数: 0

Proteomic Analysis and Reprogramming Potential of the Porcine Intra-Ooplasmic Nanovesicles. 猪卵浆内纳米囊泡的蛋白质组学分析和重编程潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-19 DOI: 10.1089/cell.2023.0050

Islam M Saadeldin, Seonggyu Bang, Abdulkadir Y Maigoro, Sung Ho Yun, Seung Ii Kim, Sanghoon Lee, Jongki Cho

{"title":"Proteomic Analysis and Reprogramming Potential of the Porcine Intra-Ooplasmic Nanovesicles.","authors":"Islam M Saadeldin, Seonggyu Bang, Abdulkadir Y Maigoro, Sung Ho Yun, Seung Ii Kim, Sanghoon Lee, Jongki Cho","doi":"10.1089/cell.2023.0050","DOIUrl":"10.1089/cell.2023.0050","url":null,"abstract":"Oocytes contain reprogramming machinery that can transform somatic cells into totipotent cells. In this study, we aimed to isolate and characterize nanovesicles from mature porcine oocytes and described them for the first time as \"intra-ooplasmic vesicles (IOVs)\". Isolated IOVs had an average diameter of 186.3 ± 10.8 nm. Proteomic analysis revealed 467 peptide reads, with the top 20 proteins related to reprogramming, antioxidative defense, cytoskeleton, heat shock proteins, and metabolism. Protein-protein interaction and gene ontology analysis indicated that these proteins were involved in various biological pathways, including protein folding, metabolism, and cellular responses to stress. Supplementing cultured fibroblasts with IOVs resulted in the expression of the pluripotency marker OCT4 and the early trophoblastic marker CDX2 and increased expression of the corresponding mRNAs together with increasing KLF4 and SALL4 expression. IOV treatment of fibroblasts for 14 consecutive days resulted in changes in cell morphology, with increased expression of ZEB2 and YBX3 as markers for epithelial-to-mesenchymal transition (EMT). These results provide a rationale for further characterization of IOVs, investigation of potential reprogramming capabilities for EMT, and the generation of induced pluripotent or oligopotent stem cells.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"238-250"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41106591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0