Cellular reprogramming最新文献_第5页

Gene Regulatory Networks: Improving Inferences with Transfer Learning. 基因调控网络：利用迁移学习改进推断。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-12-01 DOI: 10.1089/cell.2023.0095

Marcelo Tigre Moura

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Pioneer of Cloning and Inspirational Figure for Cellular Reprogramming Scientists Sir Ian Wilmut (July 7, 1944-September 10, 2023). Ian Wilmut爵士（1944年7月7日至2023年9月10日）是细胞重新编程科学家克隆和启发性图形的先驱。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 DOI: 10.1089/cell.2023.29102.mem

Carlos-Filipe Pereira

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Hepatitis B Virus x Protein Increases Cellular OCT3/4 and MYC and Facilitates Cellular Reprogramming. 乙型肝炎病毒x蛋白增加细胞OCT3/4和MYC，促进细胞重新编程。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-26 DOI: 10.1089/cell.2023.0055

Madhusudana Girija Sanal, Sarita Gupta, Rahul Saha, Nisha Vats, Shiv Kumar Sarin

{"title":"Hepatitis B Virus x Protein Increases Cellular OCT3/4 and MYC and Facilitates Cellular Reprogramming.","authors":"Madhusudana Girija Sanal, Sarita Gupta, Rahul Saha, Nisha Vats, Shiv Kumar Sarin","doi":"10.1089/cell.2023.0055","DOIUrl":"10.1089/cell.2023.0055","url":null,"abstract":"Hepatitis B virus x (HBx) is a multifunctional protein coded by the Hepatitis B virus that is involved in various cellular processes such as proliferation, cell survival/apoptosis, and histone methylation. HBx was reported to be associated with liver \"cancer stem cells.\" The stemness inducing properties of HBx could also facilitate the generation of pluripotent stem cells from somatic cells. It is well established that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using a cocktail of transcription factors called Yamanaka's factors (YFs) (OCT4, SOX2, KLF4, and MYC). The reprogramming process proceeds step-by-step with reprogramming factor chromatin interactions, transcription, and chromatin states changing during transitions. HBx is a \"broad spectrum trans-activator\" and therefore could facilitate these transitions. We electroporated low passage and high passage (difficult to reprogram) fibroblasts using YFs with and without HBx and evaluated the reprogramming efficiency. We also investigated the tri-lineage and terminal differentiation potential of iPSC derived using HBx. We found that the addition of HBx to YF improves iPSC derivation, and it increases the efficiency of iPSC generation from \"difficult or hard-to-reprogram samples\" such as high passage/senescent fibroblasts. Further, we show that HBx can substitute the key transcription factor MYC in the YF cocktail to generate iPSC. The cellular levels of OCT3/4 and MYC were increased in HBx expressing cells. Our results have practical value in improving the efficiency of pluripotent stem cell derivation from \"difficult to reprogram\" somatic cells, in addition to providing some insights into the mechanisms of liver carcinogenesis in chronic hepatitis B. To conclude, HBx improves the reprogramming efficiency of YFs. HBx increases the cellular levels of OCT3/4 and MYC.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"224-237"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41119728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor T Cells: The Intersection of Stem Cells and Immunotherapy. 诱导的多能干细胞衍生的嵌合抗原受体T细胞：干细胞与免疫治疗的交叉。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-29 DOI: 10.1089/cell.2023.0041

Mohammad Reza Lahimchi, Faezeh Maroufi, Amirhosein Maali

{"title":"Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor T Cells: The Intersection of Stem Cells and Immunotherapy.","authors":"Mohammad Reza Lahimchi, Faezeh Maroufi, Amirhosein Maali","doi":"10.1089/cell.2023.0041","DOIUrl":"10.1089/cell.2023.0041","url":null,"abstract":"Chimeric antigen receptor (CAR) T cell therapy is a promising cell-based immunotherapy applicable to various cancers. High cost of production, immune rejection, heterogeneity of cell product, limited cell source, limited expandability, and relatively long production time have created the need to achieve a universal allogeneic CAR-T cell product for \"off-the-shelf\" application. Since the innovation of induced pluripotent stem cells (iPSCs) by Yamanaka et al., extensive efforts have been made to prepare an unlimited cell source for regenerative medicine, that is, immunotherapy. In the autologous grafting approach, iPSCs prepare the desired cell source for generating autologous CAR-T cells through more accessible and available sources. In addition, generating iPSC-derived CAR-T cells is a promising approach to achieving a suitable source for producing an allogeneic CAR-T cell product. In brief, the first step is reprogramming somatic cells (accessible from peripheral blood, skin, etc.) to iPSCs. In the next step, CAR expression and T cell lineage differentiation should be applied in different arrangements. In addition, in an allogeneic manner, human leukocyte antigen/T cell receptor (TCR) deficiency should be applied in iPSC colonies. The allogeneic iPSC-derived CAR-T cell experiments showed that simultaneous performance of HLA/TCR deficiency, CAR expression, and T cell lineage differentiation could bring the production to the highest efficacy in generating allogeneic iPSC-derived CAR-T cells.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"195-211"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Efficient A·T-to-C·G Base Editing via Adenine Transversion Editors. 通过Adenine Transversion Editors进行高效的A·T到C·G基础编辑。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-19 DOI: 10.1089/cell.2023.0094

Muhammad Arslan Mahmood

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Reprogramming Cell Identity: Past Lessons, Challenges, and Future Directions. 重新编程细胞身份：过去的教训，挑战和未来的方向。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 DOI: 10.1089/cell.2023.0100

José C R Silva

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Proteomic Analysis and Reprogramming Potential of the Porcine Intra-Ooplasmic Nanovesicles. 猪卵浆内纳米囊泡的蛋白质组学分析和重编程潜力。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-19 DOI: 10.1089/cell.2023.0050

Islam M Saadeldin, Seonggyu Bang, Abdulkadir Y Maigoro, Sung Ho Yun, Seung Ii Kim, Sanghoon Lee, Jongki Cho

{"title":"Proteomic Analysis and Reprogramming Potential of the Porcine Intra-Ooplasmic Nanovesicles.","authors":"Islam M Saadeldin, Seonggyu Bang, Abdulkadir Y Maigoro, Sung Ho Yun, Seung Ii Kim, Sanghoon Lee, Jongki Cho","doi":"10.1089/cell.2023.0050","DOIUrl":"10.1089/cell.2023.0050","url":null,"abstract":"Oocytes contain reprogramming machinery that can transform somatic cells into totipotent cells. In this study, we aimed to isolate and characterize nanovesicles from mature porcine oocytes and described them for the first time as \"intra-ooplasmic vesicles (IOVs)\". Isolated IOVs had an average diameter of 186.3 ± 10.8 nm. Proteomic analysis revealed 467 peptide reads, with the top 20 proteins related to reprogramming, antioxidative defense, cytoskeleton, heat shock proteins, and metabolism. Protein-protein interaction and gene ontology analysis indicated that these proteins were involved in various biological pathways, including protein folding, metabolism, and cellular responses to stress. Supplementing cultured fibroblasts with IOVs resulted in the expression of the pluripotency marker OCT4 and the early trophoblastic marker CDX2 and increased expression of the corresponding mRNAs together with increasing KLF4 and SALL4 expression. IOV treatment of fibroblasts for 14 consecutive days resulted in changes in cell morphology, with increased expression of ZEB2 and YBX3 as markers for epithelial-to-mesenchymal transition (EMT). These results provide a rationale for further characterization of IOVs, investigation of potential reprogramming capabilities for EMT, and the generation of induced pluripotent or oligopotent stem cells.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"238-250"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41106591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Ethics of Cellular Reprogramming. 细胞重新编程的伦理学。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-09-22 DOI: 10.1089/cell.2023.0091

Anna Smajdor, Adrian Villalba

{"title":"The Ethics of Cellular Reprogramming.","authors":"Anna Smajdor, Adrian Villalba","doi":"10.1089/cell.2023.0091","DOIUrl":"10.1089/cell.2023.0091","url":null,"abstract":"Louise Brown's birth in 1978 heralded a new era not just in reproductive technology, but in the relationship between science, cells, and society. For the first time, human embryos could be created, selected, studied, manipulated, frozen, altered, or destroyed, outside the human body. But with this possibility came a plethora of ethical questions. Is it acceptable to destroy a human embryo for the purpose of research? Or to create an embryo with the specific purpose of destroying it for research? In an attempt to construct ethical and legal frameworks for the new era of cellular reprogramming, legislators and ethicists have tried to distinguish between different kinds of biological entity. We treat cells differently depending on whether they are human or animal, somatic cells or gametes, and on whether they are embryos or not. But this approach to the ethics of cellular reprogramming is doomed to failure for the simple reason that cellular reprogramming in itself destroys the distinctions that the law requires to function. In this article, we explore the historical trajectory of cellular reprogramming and its relationship with ethics and society. We suggest that the early hype of embryo research has not obviously fulfilled expectations, but since new avenues of research are continuously opening, it is hard to say definitely that these promises have been broken. We explore the forthcoming challenges posed by the creation of DNA from scratch in the laboratory, and the implications of this for understandings of identity, privacy, and reproduction. We conclude that while ethics used to seek answers in biological facts, this is no longer possible, and a new approach is required.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"190-194"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41108115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons. 人类诱导的多能干细胞衍生的周细胞作为可扩展和可编辑的来源，研究将谱系重新编程为诱导的神经元。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 Epub Date: 2023-06-27 DOI: 10.1089/cell.2023.0008

Radhika Menon, Linda Petrucci, Benjamin Lohrer, Jingzhong Zhang, Markus Schulze, Christian Schichor, Beate Winner, Jürgen Winkler, Markus J Riemenschneider, Ralf Kühn, Sven Falk, Marisa Karow

{"title":"Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons.","authors":"Radhika Menon, Linda Petrucci, Benjamin Lohrer, Jingzhong Zhang, Markus Schulze, Christian Schichor, Beate Winner, Jürgen Winkler, Markus J Riemenschneider, Ralf Kühn, Sven Falk, Marisa Karow","doi":"10.1089/cell.2023.0008","DOIUrl":"10.1089/cell.2023.0008","url":null,"abstract":"Studying human somatic cell-to-neuron conversion using primary brain-derived cells as starting cell source is hampered by limitations and variations in human biopsy material. Thus, delineating the molecular variables that allow changing the identity of somatic cells, permit adoption of neuronal phenotypes, and foster maturation of induced neurons (iNs) is challenging. Based on our previous results that pericytes derived from the adult human cerebral cortex can be directly converted into iNs (Karow et al., 2018; Karow et al., 2012), we here introduce human induced pluripotent stem cell (hiPSC)-derived pericytes (hiPSC-pericytes) as a versatile and more uniform tool to study the pericyte-to-neuron conversion process. This strategy enables us to derive scalable cell numbers and allows for engineering of the starting cell population such as introducing reporter tools before differentiation into hiPSC-pericytes and subsequent iN conversion. Harvesting the potential of this approach, we established hiPSC-derived human-human neuronal cocultures that not only allow for independent manipulation of each coculture partner but also resulted in morphologically more mature iNs. In summary, we exploit hiPSC-based methods to facilitate the analysis of human somatic cell-to-neuron conversion.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":" ","pages":"212-223"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9687149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Microscopic Analysis of Cell Fate Alteration Induced by Cell Fusion. 细胞融合诱导的细胞命运改变的显微镜分析。

IF 1.6 4区医学

Cellular reprogramming Pub Date : 2023-10-01 DOI: 10.1089/cell.2023.0073

Taisei Kumazaki, Chinatsu Yonekawa, Tomomi Tsubouchi

{"title":"Microscopic Analysis of Cell Fate Alteration Induced by Cell Fusion.","authors":"Taisei Kumazaki, Chinatsu Yonekawa, Tomomi Tsubouchi","doi":"10.1089/cell.2023.0073","DOIUrl":"10.1089/cell.2023.0073","url":null,"abstract":"In mammals, differentiated cells generally do not de-differentiate nor undergo cell fate alterations. However, they can be experimentally guided toward a different lineage. Cell fusion involving two different cell types has long been used to study this process, as this method induces cell fate alterations within hours to days in a subpopulation of fused cells, as evidenced by changes in gene-expression profiles. Despite the robustness of this system, its use has been restricted by low fusion rates and difficulty in eliminating unfused populations, thereby compromising resolution. In this study, we address these limitations by isolating fused cells using antibody-conjugated beads. This approach enables the microscopic tracking of fused cells starting as early as 5 hours after fusion. By taking advantage of species-specific FISH probes, we show that a small population of fused cells resulting from the fusion of mouse ES and human B cells, expresses OCT4 from human nuclei at levels comparable to human induced pluripotent stem cells (iPSCs) as early as 25 hours after fusion. We also show that this response can vary depending on the fusion partner. Our study broadens the usage of the cell fusion system for comprehending the mechanisms underlying cell fate alterations. These findings hold promise for diverse fields, including regenerative medicine and cancer.","PeriodicalId":9708,"journal":{"name":"Cellular reprogramming","volume":"25 5","pages":"251-259"},"PeriodicalIF":1.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41232583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0