Cell Reports Physical Science最新文献_第5页

Atomic-engineered Ru single-atom-catalyst nanoplatforms with NIR-II-triggered oxidase-peroxidase cascades for bacterial pneumonia 原子工程Ru单原子催化剂纳米平台与nir - ii触发的氧化酶-过氧化物酶级联治疗细菌性肺炎

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-10-01 DOI: 10.1016/j.xcrp.2025.102870

Mengxuan Du, Binge Huang, Songlin Xin, Liqin Wu, Xiaojun He

{"title":"Atomic-engineered Ru single-atom-catalyst nanoplatforms with NIR-II-triggered oxidase-peroxidase cascades for bacterial pneumonia","authors":"Mengxuan Du, Binge Huang, Songlin Xin, Liqin Wu, Xiaojun He","doi":"10.1016/j.xcrp.2025.102870","DOIUrl":"https://doi.org/10.1016/j.xcrp.2025.102870","url":null,"abstract":"<h2>Summary</h2> Multidrug-resistant bacterial pneumonia (MDR-BP), particularly involving biofilm eradication, presents therapeutic challenges. While reactive oxygen species (ROS)-mediated chemodynamic therapy offers antibacterial potential, limitations in biocatalytic performance and nanotherapeutic efficacy hinder traditional approaches. We developed nitrogen-doped ruthenium single-atom catalysts (Ru-N-SACs) as a dual-functional nanotherapeutic platform. This nanozyme exhibits peroxidase-mimicking catalytic activity, generating ROS selectively. Near-infrared laser stimulation further enhances its activity and provides photothermal disruption of biofilms. The resulting synergistic action achieves complete biofilm eradication and improves survival in pneumonia models. Comprehensive multi-omics analyses elucidated the therapeutic effectiveness and regulatory mechanisms of Ru-N-SACs against MDR-BP pathogenesis. This work demonstrates a paradigm combining catalytic ROS generation and photothermal therapy for managing deep-tissue infections. It advances the design of precision-engineered nanozymes within antimicrobial nanomedicine, addressing the critical need for innovative strategies against MDR pathogens.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 10","pages":"102870-102870"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147902278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The case of the vanishing perovskite layer 钙钛矿层消失的例子

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-10-01 DOI: 10.1016/j.xcrp.2025.102890

Marcin Giza, Fraser J. Angus, Wai Kin Yiu, Madeleine McRoberts, Benjamin Vella, Jingbo Wang, Aleksandra B. Djurišić, Graeme Cooke, Elisabetta Arca, Pablo Docampo

引用次数: 0

Simultaneous dual-membrane protein degradation using bivalent aptamer-delivered lysosome-targeting chimeras 利用二价适体递送的溶酶体靶向嵌合体同时降解双膜蛋白

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-10-01 DOI: 10.1016/j.xcrp.2025.102866

Xia Bai, Yuchun Wang, Xinyu Zhang, Chunxue Niu, Gang Yang, Deyu Yuan, Yun Wei, Lihua Zheng, Xu Song, Yongli Bao, Yongyun Zhao

{"title":"Simultaneous dual-membrane protein degradation using bivalent aptamer-delivered lysosome-targeting chimeras","authors":"Xia Bai, Yuchun Wang, Xinyu Zhang, Chunxue Niu, Gang Yang, Deyu Yuan, Yun Wei, Lihua Zheng, Xu Song, Yongli Bao, Yongyun Zhao","doi":"10.1016/j.xcrp.2025.102866","DOIUrl":"https://doi.org/10.1016/j.xcrp.2025.102866","url":null,"abstract":"<h2>Summary</h2> Diseases often involve multiple abnormal proteins, and degrading them simultaneously is a promising therapeutic strategy. However, transitional lysosome-targeted chimeras (LYTACs) with dedicated delivery modules typically degrade only one protein. Adding more protein of interest (POI)-targeting modules to degrade multiple targets would increase the molecular weight, leading to higher doses and side effects. Here, we report a bivalent aptamer-based delivery system for LYTAC (BideTAC), which leverages the aptamer itself as a dual-function module for targeting and delivery, eliminating the need for a dedicated delivery module and effectively reducing the molecular weight. As a proof of concept, we designed a specific BideTAC (AptHP) to simultaneously degrade PD-L1 and HER2 by conjugating anti-PD-L1 antagonistic aptamer and anti-HER2 aptamer. AptHP effectively degrades two proteins simultaneously, enhancing the therapeutic effect. Therefore, BideTAC provides a potent tool for multiple protein degradation, opening an avenue to push aptamer-based LYTAC toward applications in synergistic therapy.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 10","pages":"102866-102866"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.cell.com/cell-reports-physical-science/pdf/S2666-3864(25)00465-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced viscoelasticity as a biomechanical signature in senescent vascular endothelial cells 增强的粘弹性是衰老血管内皮细胞的生物力学特征

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-09-01 DOI: 10.1016/j.xcrp.2025.102844

Dong Ma, Jifeng Ren, Xiuqing Qian

引用次数: 2

Conformation-gated binding underlies kinetic asymmetry and negative cooperativity in ATP:cob(I)alamin adenosyltransferase. 构象门控结合是ATP:cob(I)alamin腺苷转移酶动力学不对称和负协同作用的基础。

IF 6.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 Epub Date: 2025-08-11 DOI: 10.1016/j.xcrp.2025.102768

Guangjie Yan, Manhua Pan, Aaron M Keller, Ace George Santiago, Michael Lofgren, Ruma Banerjee, Peng Chen, Tai-Yen Chen

{"title":"Conformation-gated binding underlies kinetic asymmetry and negative cooperativity in ATP:cob(I)alamin adenosyltransferase.","authors":"Guangjie Yan, Manhua Pan, Aaron M Keller, Ace George Santiago, Michael Lofgren, Ruma Banerjee, Peng Chen, Tai-Yen Chen","doi":"10.1016/j.xcrp.2025.102768","DOIUrl":"10.1016/j.xcrp.2025.102768","url":null,"abstract":"Vitamin B12 (cobalamin) is a high-value yet scarce cofactor critical for metabolic homeostasis, necessitating efficient handling mechanisms. ATP:cob(I)alamin adenosyltransferase (MMAB) plays a central role in synthesizing, delivering, and repairing 5'-deoxyadenosylcobalamin (AdoCbl), but the kinetic mechanisms regulating this process, including negative cooperativity, remain unclear. Using single-molecule relative fluorescence spectroscopy, we reveal that conformation-gated binding mechanism, involving a required structural rearrangement prior to the first cofactor association, dictates MMAB's interaction kinetics. This mechanism slows the association of a second AdoCbl, resulting in strong negative cooperativity, favoring the singly bound state, and optimizing AdoCbl handling. This gating mechanism, supported by direct observation of a kinetic intermediate, also contributes to MMAB's preferential handling of AdoCbl over hydroxocobalamin, highlighting MMAB's effective cofactor utilization, supporting bacterial survival in nutrient-limited environments. Furthermore, our approach offers a platform to study cofactor interactions, including cobalamin sensing and gene regulation, shedding light on bacterial adaptation to nutrient fluctuations.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the catalytic mechanism of the Fe(II)/2-oxoglutarate-dependent human epigenetic modifying enzyme ALKBH5. 揭示铁(II)/2-氧戊二酸盐依赖性人表观遗传修饰酶ALKBH5的催化机制。

IF 6.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 Epub Date: 2025-08-12 DOI: 10.1016/j.xcrp.2025.102779

Fathima Hameed Cherilakkudy, Midhun George Thomas, Ann Varghese, Sodiq O Waheed, Anandhu Krishnan, Vincenzo Venditti, Christopher J Schofield, Deyu Li, Christo Z Christov, Tatyana G Karabencheva-Christova

{"title":"Revealing the catalytic mechanism of the Fe(II)/2-oxoglutarate-dependent human epigenetic modifying enzyme ALKBH5.","authors":"Fathima Hameed Cherilakkudy, Midhun George Thomas, Ann Varghese, Sodiq O Waheed, Anandhu Krishnan, Vincenzo Venditti, Christopher J Schofield, Deyu Li, Christo Z Christov, Tatyana G Karabencheva-Christova","doi":"10.1016/j.xcrp.2025.102779","DOIUrl":"10.1016/j.xcrp.2025.102779","url":null,"abstract":"ALKBH5 is one of only two known human non-heme Fe(II)/2-oxoglutarate-dependent oxygenases that catalyze the demethylation of N6-methyladenine (m6A) in single-stranded mRNA, underscoring its role in diverse cancers. Unlike its homolog, the fat mass and obesity-associated protein (FTO), which oxidizes m6A to a stable N6-hydroxymethyladenine (hm6A) intermediate, ALKBH5 demethylates m6A, yielding adenine and formaldehyde as products. Here, we integrate molecular dynamics simulations and quantum mechanics/molecular mechanics methods to elucidate ALKBH5's complete catalytic mechanism. Two post-hydroxylation pathways were evaluated: a proton transfer pathway and a Schiff base formation pathway, with the former emerging as the favored mechanism. We identify second-sphere residues Lys132 and Tyr139 as essential contributors to catalysis and demonstrate how Val191 and Tyr133 modulate activity. Dynamic analyses reveal that correlated motions of structural elements such as nucleotide recognition lids NRL1 and NRL2 and increased flexibility of the NRL2 loop in the hm6A intermediate may be critical for efficient demethylation.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A zirconium-89-labeled antibody-drug conjugate PET probe for noninvasive monitoring of Nectin4 expression in breast cancer and lung cancer. 一种用于无创监测乳腺癌和肺癌中Nectin4表达的锆89标记抗体-药物偶联PET探针。

IF 6.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 DOI: 10.1016/j.xcrp.2025.102723

Wenpeng Huang, Tianyao Wang, Yutong Liang, Fangfang Chao, Qi Yang, Todd E Barnhart, Jonathan W Engle, Liming Li, Lei Kang, Weibo Cai

{"title":"A zirconium-89-labeled antibody-drug conjugate PET probe for noninvasive monitoring of Nectin4 expression in breast cancer and lung cancer.","authors":"Wenpeng Huang, Tianyao Wang, Yutong Liang, Fangfang Chao, Qi Yang, Todd E Barnhart, Jonathan W Engle, Liming Li, Lei Kang, Weibo Cai","doi":"10.1016/j.xcrp.2025.102723","DOIUrl":"10.1016/j.xcrp.2025.102723","url":null,"abstract":"Triple-negative breast cancer (TNBC) and non-small cell lung cancer (NSCLC) are aggressive solid tumors with limited treatment options. Nectin cell adhesion molecule 4 (Nectin4) is a tumor-associated antigen frequently overexpressed in these cancers, making it a promising therapeutic and imaging target. Here, we report the development and evaluation of [89Zr]Zr-desferrioxamine (DFO)-Padcev, a radiolabeled antibody-drug conjugate targeting Nectin4, for immuno-positron emission tomography (ImmunoPET) imaging. [89Zr]Zr-DFO-Padcev is synthesized with a radiochemical yield of 88.87% ± 2.59% and a radiochemical purity above 99%. ImmunoPET imaging successfully visualizes Nectin4-positive tumors in TNBC (MDA-MB-468) and NSCLC (H1975) models as early as 6 h post-injection, with uptake progressively increasing and peaking at 48 h (14.57 ± 1.94 and 9.50 ± 0.76 %ID/g, respectively). Minimal tumor uptake is observed in blocking and Nectin4-negative controls, confirming specificity. Complementary fluorescence imaging further reveals the in vivo distribution of Padcev, providing valuable insights into optimal therapeutic time windows.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":""},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12806174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A percolating path to green iron. 一条通往绿铁的渗透路径。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 DOI: 10.1016/j.xcrp.2025.102729

Subhechchha Paul, Brinthan Kanesalingam, Yan Ma, Julie Villanova, Guillermo Requena, Stanley Chidubem Akpu, Dierk Raabe, Ilenia Battiato, Leora Dresselhaus-Marais

{"title":"A percolating path to green iron.","authors":"Subhechchha Paul, Brinthan Kanesalingam, Yan Ma, Julie Villanova, Guillermo Requena, Stanley Chidubem Akpu, Dierk Raabe, Ilenia Battiato, Leora Dresselhaus-Marais","doi":"10.1016/j.xcrp.2025.102729","DOIUrl":"https://doi.org/10.1016/j.xcrp.2025.102729","url":null,"abstract":"About 1.9 gigatons of steel is produced every year, emitting 8% (3.6 gigatons) of global CO2 in the process. More than 50% of the CO2 emissions come from a single step of steel production, known as ironmaking. Hydrogen-based direct reduction (HyDR) of iron oxide to iron has emerged as an emission-free ironmaking alternative. However, multiple physical and chemical phenomena ranging from nanometers to meters inside HyDR reactors alter the microstructure and pore networks in iron oxide pellets, in ways that resist gaseous transport of H2/H2O, slow reaction rates, and disrupt continuous reactor operation. Using synchrotron nano X-ray computed tomography and percolation theory, we quantify the evolution of pores in iron oxide pellets and demonstrate how nanoscale pore connectivity influences micro- and macroscale flow properties such as permeability, diffusivity, and tortuosity. Our modeling framework connects disparate scales and offers opportunities to accelerate HyDR.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":"102729"},"PeriodicalIF":7.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binding affinity and binding sites to albumin reshape efficacy of gemcitabine prodrugs 与白蛋白的结合亲和力和结合位点重塑吉西他滨前药的疗效

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-01 DOI: 10.1016/j.xcrp.2025.102775

Haiyu Zhao, Wenxiao Li, Xuan Li, Zhiyu Kuang, Shanhui Xu, Zhenzhen Zhao, Jin Sun, Zhonggui He, Chunwang Fu, Yixin Sun, Bingjun Sun

引用次数: 0

Reaction-driven self-adapting Pd/Nb2O5 steering alkyne semi-hydrogenation 反应驱动自适应Pd/Nb2O5转向炔半加氢

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-01 DOI: 10.1016/j.xcrp.2025.102770

Zhongzhe Wei, Xu Liu, Mingxuan Wang, Yao Lv, Songtao Huang, Jing Wang, Ming Jiang, Zhixiang Yang, Zihao Yao, Sheng Dai, Xiao‐Nian Li, Jianguo Wang

引用次数: 2