使用杜拉霉素固定金纳米棒探针对细胞凋亡进行生物发光和光声双模式成像

IF 7.9 2区 综合性期刊 Q1 CHEMISTRY, MULTIDISCIPLINARY
Jingyu Zhang, Bin Guo, Yiyi Jiang, Xiaorui Shi, Chong Hu, Zihao Jiao, Fu Wang
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引用次数: 0

摘要

磷脂酰乙醇胺(PE)转位被认为是细胞凋亡的标志性事件。开发以 PE 为靶点的非侵入性多模态探针用于细胞凋亡检测前景广阔。在此,我们开发了一种用于检测肿瘤细胞凋亡的双模态成像探针--杜拉霉素-Fluc-AuNRs(DFA)。DFA 是通过将杜拉霉素肽和萤火虫荧光素酶(Fluc)重组蛋白与金纳米棒(AuNRs)连接而制成的。杜拉霉素对 PE 具有高亲和力,而萤火虫荧光素酶能产生强大的生物发光信号,AuNRs 则通过光声转换提高成像分辨率。DFA 探针在细胞和小鼠体内均表现出低毒性,展示了其体内应用的潜力。在 A549 和 4T1 细胞系中,DFA 探针的生物发光信号随着多柔比星(Dox)诱导的细胞凋亡程度而增加。在小鼠水平上,Dox 诱导凋亡的小鼠表现出更高的生物发光和光声成像信号。因此,这种双模式生物发光/光声成像平台在检测细胞凋亡和提供高性能成像信息方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bioluminescence and photoacoustic dual-modality imaging of apoptosis using a duramycin-immobilized gold nanorod probe

Bioluminescence and photoacoustic dual-modality imaging of apoptosis using a duramycin-immobilized gold nanorod probe

Phosphatidylethanolamine (PE) translocation is considered a hallmark event of cellular apoptosis. The development of non-invasive multi-modality probes targeting PE for apoptosis detection holds great promise. Here, we develop a dual-modality imaging probe, duramycin-Fluc-AuNRs (DFA), for detecting apoptosis in tumor cells. DFA is created by linking duramycin peptide and firefly luciferase (Fluc) recombinant protein to gold nanorods (AuNRs). Duramycin exhibits high affinity for PE, while Fluc produces a robust bioluminescence signal, and AuNRs enhance imaging resolution through photoacoustic conversion. The DFA probe demonstrates low toxicity in both cells and mice, showcasing its potential for in vivo applications. In A549 and 4T1 cell lines, the bioluminescence signal of the DFA probe increases with the degree of doxorubicin (Dox)-induced apoptosis. At the mouse level, mice with Dox-triggered apoptosis exhibit higher bioluminescence and photoacoustic imaging signals. Thus, this dual-modality bioluminescence/photoacoustic imaging platform holds significant potential for detecting cellular apoptosis and providing high-performance imaging information.

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来源期刊
Cell Reports Physical Science
Cell Reports Physical Science Energy-Energy (all)
CiteScore
11.40
自引率
2.20%
发文量
388
审稿时长
62 days
期刊介绍: Cell Reports Physical Science, a premium open-access journal from Cell Press, features high-quality, cutting-edge research spanning the physical sciences. It serves as an open forum fostering collaboration among physical scientists while championing open science principles. Published works must signify significant advancements in fundamental insight or technological applications within fields such as chemistry, physics, materials science, energy science, engineering, and related interdisciplinary studies. In addition to longer articles, the journal considers impactful short-form reports and short reviews covering recent literature in emerging fields. Continually adapting to the evolving open science landscape, the journal reviews its policies to align with community consensus and best practices.
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