Cell Reports Physical Science最新文献

Conformation-gated binding underlies kinetic asymmetry and negative cooperativity in ATP:cob(I)alamin adenosyltransferase. 构象门控结合是ATP:cob(I)alamin腺苷转移酶动力学不对称和负协同作用的基础。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 Epub Date: 2025-08-11 DOI: 10.1016/j.xcrp.2025.102768

Guangjie Yan, Manhua Pan, Aaron M Keller, Ace George Santiago, Michael Lofgren, Ruma Banerjee, Peng Chen, Tai-Yen Chen

{"title":"Conformation-gated binding underlies kinetic asymmetry and negative cooperativity in ATP:cob(I)alamin adenosyltransferase.","authors":"Guangjie Yan, Manhua Pan, Aaron M Keller, Ace George Santiago, Michael Lofgren, Ruma Banerjee, Peng Chen, Tai-Yen Chen","doi":"10.1016/j.xcrp.2025.102768","DOIUrl":"10.1016/j.xcrp.2025.102768","url":null,"abstract":"Vitamin B12 (cobalamin) is a high-value yet scarce cofactor critical for metabolic homeostasis, necessitating efficient handling mechanisms. ATP:cob(I)alamin adenosyltransferase (MMAB) plays a central role in synthesizing, delivering, and repairing 5'-deoxyadenosylcobalamin (AdoCbl), but the kinetic mechanisms regulating this process, including negative cooperativity, remain unclear. Using single-molecule relative fluorescence spectroscopy, we reveal that conformation-gated binding mechanism, involving a required structural rearrangement prior to the first cofactor association, dictates MMAB's interaction kinetics. This mechanism slows the association of a second AdoCbl, resulting in strong negative cooperativity, favoring the singly bound state, and optimizing AdoCbl handling. This gating mechanism, supported by direct observation of a kinetic intermediate, also contributes to MMAB's preferential handling of AdoCbl over hydroxocobalamin, highlighting MMAB's effective cofactor utilization, supporting bacterial survival in nutrient-limited environments. Furthermore, our approach offers a platform to study cofactor interactions, including cobalamin sensing and gene regulation, shedding light on bacterial adaptation to nutrient fluctuations.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the catalytic mechanism of the Fe(II)/2-oxoglutarate-dependent human epigenetic modifying enzyme ALKBH5. 揭示铁(II)/2-氧戊二酸盐依赖性人表观遗传修饰酶ALKBH5的催化机制。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 Epub Date: 2025-08-12 DOI: 10.1016/j.xcrp.2025.102779

Fathima Hameed Cherilakkudy, Midhun George Thomas, Ann Varghese, Sodiq O Waheed, Anandhu Krishnan, Vincenzo Venditti, Christopher J Schofield, Deyu Li, Christo Z Christov, Tatyana G Karabencheva-Christova

{"title":"Revealing the catalytic mechanism of the Fe(II)/2-oxoglutarate-dependent human epigenetic modifying enzyme ALKBH5.","authors":"Fathima Hameed Cherilakkudy, Midhun George Thomas, Ann Varghese, Sodiq O Waheed, Anandhu Krishnan, Vincenzo Venditti, Christopher J Schofield, Deyu Li, Christo Z Christov, Tatyana G Karabencheva-Christova","doi":"10.1016/j.xcrp.2025.102779","DOIUrl":"10.1016/j.xcrp.2025.102779","url":null,"abstract":"ALKBH5 is one of only two known human non-heme Fe(II)/2-oxoglutarate-dependent oxygenases that catalyze the demethylation of N6-methyladenine (m6A) in single-stranded mRNA, underscoring its role in diverse cancers. Unlike its homolog, the fat mass and obesity-associated protein (FTO), which oxidizes m6A to a stable N6-hydroxymethyladenine (hm6A) intermediate, ALKBH5 demethylates m6A, yielding adenine and formaldehyde as products. Here, we integrate molecular dynamics simulations and quantum mechanics/molecular mechanics methods to elucidate ALKBH5's complete catalytic mechanism. Two post-hydroxylation pathways were evaluated: a proton transfer pathway and a Schiff base formation pathway, with the former emerging as the favored mechanism. We identify second-sphere residues Lys132 and Tyr139 as essential contributors to catalysis and demonstrate how Val191 and Tyr133 modulate activity. Dynamic analyses reveal that correlated motions of structural elements such as nucleotide recognition lids NRL1 and NRL2 and increased flexibility of the NRL2 loop in the hm6A intermediate may be critical for efficient demethylation.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12439684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A percolating path to green iron. 一条通往绿铁的渗透路径。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-08-20 DOI: 10.1016/j.xcrp.2025.102729

Subhechchha Paul, Brinthan Kanesalingam, Yan Ma, Julie Villanova, Guillermo Requena, Stanley Chidubem Akpu, Dierk Raabe, Ilenia Battiato, Leora Dresselhaus-Marais

{"title":"A percolating path to green iron.","authors":"Subhechchha Paul, Brinthan Kanesalingam, Yan Ma, Julie Villanova, Guillermo Requena, Stanley Chidubem Akpu, Dierk Raabe, Ilenia Battiato, Leora Dresselhaus-Marais","doi":"10.1016/j.xcrp.2025.102729","DOIUrl":"https://doi.org/10.1016/j.xcrp.2025.102729","url":null,"abstract":"About 1.9 gigatons of steel is produced every year, emitting 8% (3.6 gigatons) of global CO2 in the process. More than 50% of the CO2 emissions come from a single step of steel production, known as ironmaking. Hydrogen-based direct reduction (HyDR) of iron oxide to iron has emerged as an emission-free ironmaking alternative. However, multiple physical and chemical phenomena ranging from nanometers to meters inside HyDR reactors alter the microstructure and pore networks in iron oxide pellets, in ways that resist gaseous transport of H2/H2O, slow reaction rates, and disrupt continuous reactor operation. Using synchrotron nano X-ray computed tomography and percolation theory, we quantify the evolution of pores in iron oxide pellets and demonstrate how nanoscale pore connectivity influences micro- and macroscale flow properties such as permeability, diffusivity, and tortuosity. Our modeling framework connects disparate scales and offers opportunities to accelerate HyDR.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 8","pages":"102729"},"PeriodicalIF":7.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12374072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Sabatier principle governs the performance of self-sufficient heterogeneous biocatalysts for redox biotransformations. 萨巴蒂尔原理支配着自给自足的多相生物催化剂氧化还原生物转化的性能。

IF 7.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-16 DOI: 10.1016/j.xcrp.2025.102694

Eleftheria Diamanti, Ainhoa Oliden-Sánchez, Daniel Grajales-Hernández, Daniel Andrés-Sanz, Rut Fernández-Marín, Daniel Padro, Jesús Ruíz-Cabello, Ronen Zangi, Fernando López-Gallego

{"title":"The Sabatier principle governs the performance of self-sufficient heterogeneous biocatalysts for redox biotransformations.","authors":"Eleftheria Diamanti, Ainhoa Oliden-Sánchez, Daniel Grajales-Hernández, Daniel Andrés-Sanz, Rut Fernández-Marín, Daniel Padro, Jesús Ruíz-Cabello, Ronen Zangi, Fernando López-Gallego","doi":"10.1016/j.xcrp.2025.102694","DOIUrl":"10.1016/j.xcrp.2025.102694","url":null,"abstract":"Self-sufficient heterogeneous biocatalysts (ssHBs), in which enzymes and cofactors are coimmobilized on the same support, provide in situ cofactor regeneration and reduce operating costs. However, the underlying mechanisms remain poorly understood. Here, we present a theoretical model for ssHBs consisting of NAD(P)H-dependent dehydrogenases immobilized on porous agarose-based materials with cofactors coimmobilized through electrostatic interactions via a cationic polymer coating. This model links enzyme activity to cofactor-polymer binding thermodynamics and demonstrates that ssHBs obey the Sabatier principle, where maximum catalytic efficiency is achieved at an intermediate binding strength. Adjustment of pH and ionic strength modulates this interaction, and the resulting activity exhibits the predicted volcano plot. Depending on the reaction conditions, electrostatic complexation is influenced, resulting in the formation of a dense, liquid-like phase inside the particles. Our study directly confirms the Sabatier principle in ssHBs and highlights the crucial role of cofactor binding thermodynamics in optimizing biocatalysis for chemical applications.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":"102694"},"PeriodicalIF":7.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dimerization domain of SARS-CoV-2 nucleocapsid protein is partially disordered and forms a dynamic high-affinity dimer. SARS-CoV-2核衣壳蛋白二聚化结构域部分紊乱，形成动态高亲和力二聚体。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-16 DOI: 10.1016/j.xcrp.2025.102695

Jasmine Cubuk, J Jeremías Incicco, Kathleen B Hall, Alex S Holehouse, Melissa D Stuchell-Brereton, Andrea Soranno

{"title":"The dimerization domain of SARS-CoV-2 nucleocapsid protein is partially disordered and forms a dynamic high-affinity dimer.","authors":"Jasmine Cubuk, J Jeremías Incicco, Kathleen B Hall, Alex S Holehouse, Melissa D Stuchell-Brereton, Andrea Soranno","doi":"10.1016/j.xcrp.2025.102695","DOIUrl":"10.1016/j.xcrp.2025.102695","url":null,"abstract":"The SARS-CoV-2 nucleocapsid (N) drives the compaction and packaging of the viral genome. Here, we focused on quantifying the mechanisms that control dimer formation utilizing single-molecule Förster resonance energy transfer to investigate the conformations and energetics of the dimerization domain in the context of the full-length protein. Under monomeric conditions, we observed significantly expanded configurations of the dimerization domain (compared to the folded dimer structure), which is consistent with a dynamic conformational ensemble. The addition of unlabeled protein stabilizes a folded dimer configuration with a high mean transfer efficiency, which is in agreement with predictions based on known structures. Dimerization is characterized by a dissociation constant of ~12 nM at 23°C and is driven by strong enthalpic interactions between the two protein subunits, which originate from the coupled folding and binding. We propose that the retained flexibility of the dimer can affect its interaction with RNA and phase separation propensity.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12385586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excitation and polarization of isolated neurons by high-frequency sine waves for temporal interference stimulation. 高频正弦波对孤立神经元的时间干扰刺激的激发和极化。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-16 Epub Date: 2025-06-16 DOI: 10.1016/j.xcrp.2025.102660

Iurii Semenov, Vitalii Kim, Giedre Silkuniene, Andrei G Pakhomov

{"title":"Excitation and polarization of isolated neurons by high-frequency sine waves for temporal interference stimulation.","authors":"Iurii Semenov, Vitalii Kim, Giedre Silkuniene, Andrei G Pakhomov","doi":"10.1016/j.xcrp.2025.102660","DOIUrl":"10.1016/j.xcrp.2025.102660","url":null,"abstract":"The capacity of temporal interference (TI) stimulation to target deep brain regions without affecting nearby surface electrodes remains uncertain. Using artifact-free optical recording, we compare excitation patterns and thresholds in hippocampal neurons stimulated by \"pure\" and amplitude-modulated sine waves, representing TI waveforms near electrodes and at the target, respectively. We show that pure 2- and 20-kHz sine waves induce repetitive firing at rates that increase up to 60-90 Hz with stronger electric fields. Beyond this limit, action potentials merge into sustained depolarization, resulting in an excitation block. Modulating the sine waves at 20 Hz aligns firing with amplitude \"beats\" and prevents the excitation block but does not lower excitation thresholds. Thus, off-target TI effects appear unavoidable, though the patterns of neuronal excitation and downstream effects may differ from those at the target. We further analyze membrane charging and relaxation kinetics at nanoscale resolution and confirm an excitation mechanism independent of envelope extraction.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robustness in biomolecular simulations: Addressing challenges in data generation, analysis, and curation. 生物分子模拟中的稳健性：解决数据生成、分析和管理方面的挑战。

IF 7.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-05-21 Epub Date: 2025-04-30 DOI: 10.1016/j.xcrp.2025.102566

Anne M Brown, Justin A Lemkul

引用次数: 0

G-quadruplex and i-motif DNA structures form in the promoter of the key innate immune adaptor MYD88. g -四重体和i基序DNA结构在关键先天免疫适配器MYD88的启动子中形成。

IF 7.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-05-21 Epub Date: 2025-04-28 DOI: 10.1016/j.xcrp.2025.102560

Susie Brown, Kennith Swafford, Mason McCrury, Farhana Nasrin, Carlon Q Gragg, Arundhati Chavan, Samrat Roy Choudhury, Jonathan Dickerhoff, Danzhou Yang, Samantha Kendrick

{"title":"G-quadruplex and i-motif DNA structures form in the promoter of the key innate immune adaptor MYD88.","authors":"Susie Brown, Kennith Swafford, Mason McCrury, Farhana Nasrin, Carlon Q Gragg, Arundhati Chavan, Samrat Roy Choudhury, Jonathan Dickerhoff, Danzhou Yang, Samantha Kendrick","doi":"10.1016/j.xcrp.2025.102560","DOIUrl":"10.1016/j.xcrp.2025.102560","url":null,"abstract":"Innate immune responses rely on a critical adaptor protein, MYD88, to bridge extracellular inflammatory signals and transcription factor networks inside the cell. Dysregulation of MYD88 is associated with immunodeficiencies, autoimmunity, and cancer. Here, we identify a stretch of guanine/cytosine-rich DNA in the MYD88 promoter capable of adopting stable G-quadruplex and i-Motif structures. Molecular characterization of the i-motif reveals a unique folding pattern with asymmetric lateral loop sizes, a transition pH in line with previously documented i-motifs, and in vitro recognition by the chromatin insulator/transcription factor (CTCF). In exploring the transcriptional role and therapeutic potential of the MYD88 structures, we show the known G-quadruplex ligand, TMPyP4, destabilizes the i-motif, stabilizes the G-quadruplex, and promotes MYD88 expression. A ligand, 33353, from the National Cancer Institute (NCI) Diversity Set, also differentially interacts with the two structures yet represses MYD88. This work discovers DNA structures in MYD88 that can be pharmacologically leveraged for their ability to control gene expression.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 5","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12245167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemiosmotic ATP synthesis by minimal protocells. 最小原始细胞的化学渗透ATP合成。

IF 7.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-03-19 DOI: 10.1016/j.xcrp.2025.102461

Fanchen Yu, Jinbo Fei, Yi Jia, Tonghui Wang, William F Martin, Junbai Li

{"title":"Chemiosmotic ATP synthesis by minimal protocells.","authors":"Fanchen Yu, Jinbo Fei, Yi Jia, Tonghui Wang, William F Martin, Junbai Li","doi":"10.1016/j.xcrp.2025.102461","DOIUrl":"10.1016/j.xcrp.2025.102461","url":null,"abstract":"Energy conservation is crucial to life's origin and evolution. The common ancestor of all cells used ATP synthase to convert proton gradients into ATP. However, pumps generating proton gradients and lipids maintaining proton gradients are not universally conserved across all lineages. A solution to this paradox is that ancestral ATP synthase could harness naturally formed geochemical ion gradients with simpler environmentally provided precursors preceding both proton pumps and biogenic membranes. This runs counter to traditional views that phospholipid bilayers are required to maintain proton gradients. Here, we show that fatty acid membranes can maintain sufficient proton gradients to synthesize ATP by ATP synthase under the steep pH and temperature gradients observed in hydrothermal vent systems. These findings shed substantial light on early membrane bioenergetics, uncovering a functional intermediate in the evolution of chemiosmotic ATP synthesis during protocellular stages postdating the ATP synthase's origin but preceding the advent of enzymatically synthesized cell membranes.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 3","pages":"102461"},"PeriodicalIF":7.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transporter excess and clustering facilitate adaptor protein shuttling for bacterial efflux. 转运蛋白过剩和聚集有利于细菌外排的衔接蛋白穿梭。

IF 7.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-02-19 Epub Date: 2025-02-12 DOI: 10.1016/j.xcrp.2025.102441

Wenyao Zhang, Christine E Harper, Junsung Lee, Bing Fu, Malissa Ramsukh, Christopher J Hernandez, Peng Chen

{"title":"Transporter excess and clustering facilitate adaptor protein shuttling for bacterial efflux.","authors":"Wenyao Zhang, Christine E Harper, Junsung Lee, Bing Fu, Malissa Ramsukh, Christopher J Hernandez, Peng Chen","doi":"10.1016/j.xcrp.2025.102441","DOIUrl":"10.1016/j.xcrp.2025.102441","url":null,"abstract":"Multidrug efflux pumps confer not only antibiotic resistance to bacteria but also cell proliferation. In gram-negative bacteria, the ATP-binding cassette (ABC)-family transporter MacB, the adaptor protein MacA, and the outer membrane protein TolC form the MacA6:MacB2:TolC3 assembly to extrude antibiotics and virulence factors. Here, using quantitative single-molecule single-cell imaging, we uncover that, in E. coli cells, there is a large excess of MacB (and TolC) driving the limiting adaptor protein MacA mostly into the MacAB-TolC assembly. Moreover, the excess MacB transporters can dynamically cluster around the assembly, and MacA can dynamically disassemble from the MacAB-TolC assembly, leading to an adaptor protein shuttling mechanism for efficient substrate sequestration from the periplasm toward efflux. We further show that both MacB clustering and MacAB-TolC assembly can be perturbed chemically or physically via microfluidics-based extrusion loading for compromised antibiotic tolerance. These insights may provide opportunities for countering the activities of multidrug efflux systems for antimicrobial treatments.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 2","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0