Cell Reports Physical Science最新文献_第6页

The Sabatier principle governs the performance of self-sufficient heterogeneous biocatalysts for redox biotransformations. 萨巴蒂尔原理支配着自给自足的多相生物催化剂氧化还原生物转化的性能。

IF 7.9 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-16 DOI: 10.1016/j.xcrp.2025.102694

Eleftheria Diamanti, Ainhoa Oliden-Sánchez, Daniel Grajales-Hernández, Daniel Andrés-Sanz, Rut Fernández-Marín, Daniel Padro, Jesús Ruíz-Cabello, Ronen Zangi, Fernando López-Gallego

{"title":"The Sabatier principle governs the performance of self-sufficient heterogeneous biocatalysts for redox biotransformations.","authors":"Eleftheria Diamanti, Ainhoa Oliden-Sánchez, Daniel Grajales-Hernández, Daniel Andrés-Sanz, Rut Fernández-Marín, Daniel Padro, Jesús Ruíz-Cabello, Ronen Zangi, Fernando López-Gallego","doi":"10.1016/j.xcrp.2025.102694","DOIUrl":"10.1016/j.xcrp.2025.102694","url":null,"abstract":"Self-sufficient heterogeneous biocatalysts (ssHBs), in which enzymes and cofactors are coimmobilized on the same support, provide in situ cofactor regeneration and reduce operating costs. However, the underlying mechanisms remain poorly understood. Here, we present a theoretical model for ssHBs consisting of NAD(P)H-dependent dehydrogenases immobilized on porous agarose-based materials with cofactors coimmobilized through electrostatic interactions via a cationic polymer coating. This model links enzyme activity to cofactor-polymer binding thermodynamics and demonstrates that ssHBs obey the Sabatier principle, where maximum catalytic efficiency is achieved at an intermediate binding strength. Adjustment of pH and ionic strength modulates this interaction, and the resulting activity exhibits the predicted volcano plot. Depending on the reaction conditions, electrostatic complexation is influenced, resulting in the formation of a dense, liquid-like phase inside the particles. Our study directly confirms the Sabatier principle in ssHBs and highlights the crucial role of cofactor binding thermodynamics in optimizing biocatalysis for chemical applications.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":"102694"},"PeriodicalIF":7.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dimerization domain of SARS-CoV-2 nucleocapsid protein is partially disordered and forms a dynamic high-affinity dimer. SARS-CoV-2核衣壳蛋白二聚化结构域部分紊乱，形成动态高亲和力二聚体。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-16 DOI: 10.1016/j.xcrp.2025.102695

Jasmine Cubuk, J Jeremías Incicco, Kathleen B Hall, Alex S Holehouse, Melissa D Stuchell-Brereton, Andrea Soranno

{"title":"The dimerization domain of SARS-CoV-2 nucleocapsid protein is partially disordered and forms a dynamic high-affinity dimer.","authors":"Jasmine Cubuk, J Jeremías Incicco, Kathleen B Hall, Alex S Holehouse, Melissa D Stuchell-Brereton, Andrea Soranno","doi":"10.1016/j.xcrp.2025.102695","DOIUrl":"10.1016/j.xcrp.2025.102695","url":null,"abstract":"The SARS-CoV-2 nucleocapsid (N) drives the compaction and packaging of the viral genome. Here, we focused on quantifying the mechanisms that control dimer formation utilizing single-molecule Förster resonance energy transfer to investigate the conformations and energetics of the dimerization domain in the context of the full-length protein. Under monomeric conditions, we observed significantly expanded configurations of the dimerization domain (compared to the folded dimer structure), which is consistent with a dynamic conformational ensemble. The addition of unlabeled protein stabilizes a folded dimer configuration with a high mean transfer efficiency, which is in agreement with predictions based on known structures. Dimerization is characterized by a dissociation constant of ~12 nM at 23°C and is driven by strong enthalpic interactions between the two protein subunits, which originate from the coupled folding and binding. We propose that the retained flexibility of the dimer can affect its interaction with RNA and phase separation propensity.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12385586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144944392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excitation and polarization of isolated neurons by high-frequency sine waves for temporal interference stimulation. 高频正弦波对孤立神经元的时间干扰刺激的激发和极化。

IF 7.3 2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-16 Epub Date: 2025-06-16 DOI: 10.1016/j.xcrp.2025.102660

Iurii Semenov, Vitalii Kim, Giedre Silkuniene, Andrei G Pakhomov

{"title":"Excitation and polarization of isolated neurons by high-frequency sine waves for temporal interference stimulation.","authors":"Iurii Semenov, Vitalii Kim, Giedre Silkuniene, Andrei G Pakhomov","doi":"10.1016/j.xcrp.2025.102660","DOIUrl":"10.1016/j.xcrp.2025.102660","url":null,"abstract":"The capacity of temporal interference (TI) stimulation to target deep brain regions without affecting nearby surface electrodes remains uncertain. Using artifact-free optical recording, we compare excitation patterns and thresholds in hippocampal neurons stimulated by \"pure\" and amplitude-modulated sine waves, representing TI waveforms near electrodes and at the target, respectively. We show that pure 2- and 20-kHz sine waves induce repetitive firing at rates that increase up to 60-90 Hz with stronger electric fields. Beyond this limit, action potentials merge into sustained depolarization, resulting in an excitation block. Modulating the sine waves at 20 Hz aligns firing with amplitude \"beats\" and prevents the excitation block but does not lower excitation thresholds. Thus, off-target TI effects appear unavoidable, though the patterns of neuronal excitation and downstream effects may differ from those at the target. We further analyze membrane charging and relaxation kinetics at nanoscale resolution and confirm an excitation mechanism independent of envelope extraction.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":""},"PeriodicalIF":7.3,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144783605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence sensing of nitro explosives based on deep learning 基于深度学习的硝基炸药荧光传感

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-01 DOI: 10.1016/j.xcrp.2025.102690

Jingyi Xiong, J.-Y. Zhang, Xufan Zheng, Tianyu Hu, Huimin Xiang, Yuda Li, Biao Xiao, Xunchang Wang, Renqiang Yang

引用次数: 0

Blue light excitation at room temperature in lead-free double perovskite with Yb doping 掺杂镱的无铅双钙钛矿室温蓝光激发

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-01 DOI: 10.1016/j.xcrp.2025.102679

Xiaokai Gong, Xiaosong Zhang, Qian Li, Xing-Yao Zhao, Lina Kong, Enze Jin, Baozeng Zhou, Jianping Xu, Lan Li

引用次数: 1

Optimizing nitrogen doping in cobalt nitrogen carbon catalysts for enhanced peroxymonosulfate activation 优化钴氮碳催化剂中氮掺杂以增强过氧单硫酸盐活化

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-01 DOI: 10.1016/j.xcrp.2025.102689

Huanran Miao, Huai Wang, Qikui Fan, Zhimao Yang, Chuncai Kong

{"title":"Optimizing nitrogen doping in cobalt nitrogen carbon catalysts for enhanced peroxymonosulfate activation","authors":"Huanran Miao, Huai Wang, Qikui Fan, Zhimao Yang, Chuncai Kong","doi":"10.1016/j.xcrp.2025.102689","DOIUrl":"https://doi.org/10.1016/j.xcrp.2025.102689","url":null,"abstract":"<h2>Summary</h2> The effects of nitrogen doping and electronic modifications on peroxymonosulfate (PMS) activation still lack investigation. Herein, we investigate the impact of different nitrogen doping types on the PMS activation process. We develop CoNC catalysts (Co3O4-decorated nitrogen-doped carbon catalysts) with varying ratios of nitrogen doping types, including pyridinic, pyrrolic, and graphitic nitrogen. Both experimental and theoretical results demonstrate that, among all doping types, pyridinic nitrogen significantly promotes the growth of active sites and enhances the activation of PMS. CoNC achieves high degradation efficiencies of volatile organic compounds (VOCs) mixture: 99.3% for styrene and 91.8% for toluene. Long-term stability tests confirm that CoNC maintains high performance over six reaction cycles. Density functional theory (DFT) calculations reveal the PMS adsorption sites on CoNC and outline its decomposition pathway. This study paves the way for the design of efficient catalysts through electronic structure regulation, advancing the potential for PMS-based air pollution control in complex environments.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 7","pages":"102689-102689"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.cell.com/cell-reports-physical-science/pdf/S2666-3864(25)00288-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An engineered living biofilm system for sustainable conversion of CO2 into polyhydroxyalkanoates 一种工程活生物膜系统，用于可持续地将二氧化碳转化为聚羟基烷酸酯

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-07-01 DOI: 10.1016/j.xcrp.2025.102676

Weiqiang Wang, Weifeng Zhang, Fengjiao Chu, Tingting Xiao, Dongdong Hu, Pengyi Tang, Jiajia Gao, Honghua Ge

引用次数: 4

Synergetic integration of solar heating and radiative cooling for self-adaptive thermal regulation 太阳能加热和辐射冷却协同集成的自适应热调节

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-06-20 DOI: 10.1016/j.xcrp.2025.102661

Kegui Lu, Guang Chen, Wenshuo Zhang, Lanxin Li, Cheng Jin, Jiahao Ni, Ken Chen, Ye‐Zi You, Gang Pei, Bin Zhao

引用次数: 5

Facile silane modification of titanium dental implants achieves a consensus of osseointegration and antibacterial activity 易硅烷改性钛牙种植体达到了骨整合和抗菌活性的一致

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-06-01 DOI: 10.1016/j.xcrp.2025.102625

Yuanchen Ma, Guoqing Wen, Hanlin Song, Meng Yu, Junzhe Sheng, Jing Wang, Chen Dong, Hai-Miao Wu, Yang Liu, Xu Chen, Ziqi Liang

引用次数: 1

Unlocking high lithium-ion transport in solid polymer electrolytes with dynamic hydrogen-bonded zinc-adeninate framework 用动态氢键锌-腺苷酸框架解锁固体聚合物电解质中的高锂离子输运

2区综合性期刊

Cell Reports Physical Science Pub Date : 2025-06-01 DOI: 10.1016/j.xcrp.2025.102624

Yi Li, Dongming Cheng, Jinya Tian, Yaling Zhu, Henghui Xu, Yunhui Huang, Xiaodong Chi

{"title":"Unlocking high lithium-ion transport in solid polymer electrolytes with dynamic hydrogen-bonded zinc-adeninate framework","authors":"Yi Li, Dongming Cheng, Jinya Tian, Yaling Zhu, Henghui Xu, Yunhui Huang, Xiaodong Chi","doi":"10.1016/j.xcrp.2025.102624","DOIUrl":"https://doi.org/10.1016/j.xcrp.2025.102624","url":null,"abstract":"<h2>Summary</h2> Fast and selective lithium-ion transport is crucial for advancing solid-state electrolytes in lithium metal batteries. While porous materials with tunable Li+ transport pathways show promise, challenges remain in optimizing transport kinetics, stability, and interfacial compatibility. Herein, we present a solid-state polymer electrolyte (SPE) incorporating a hydrogen-bonded zinc-adeninate framework (ZAF) with abundant carbonyl and hydrogen-bonding functionalities, providing additional hopping sites for Li+ transport. The ZAF-poly(ethylene oxide) (PEO) electrolyte achieves high ionic conductivity (1.37 mS cm−1 at 60°C), a lithium-ion transfer number of 0.56, and a wide electrochemical window (5.04 V). It delivers exceptional cycling stability, with Li|ZAF-PEO|Li symmetric cells cycling for 1,900 h at 0.2 mA cm−2 and Li|ZAF-PEO|LFP cells stable for over 800 cycles at 1 C with 99.8% coulombic efficiency. Additionally, the pouch cell maintains excellent safety under mechanical stresses, such as bending and shearing. These results highlight the potential of ZAF-based SPEs for safer, high-performance lithium metal batteries.","PeriodicalId":9703,"journal":{"name":"Cell Reports Physical Science","volume":"6 6","pages":"102624-102624"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.cell.com/action/showPdf?pii=S2666386425002231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13