Cell Stress & Chaperones最新文献_第9页

Unveiling a differential metabolite modulation of sorghum varieties under increasing tunicamycin-induced endoplasmic reticulum stress. 揭示了在膜霉素诱导的内质网应激增加下高粱品种的差异代谢产物调节。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-09-29 DOI: 10.1007/s12192-023-01382-5

Francisco Lucas Pacheco Cavalcante, Sávio Justino da Silva, Lineker de Sousa Lopes, Stelamaris de Oliveira Paula-Marinho, Maria Izabel Florindo Guedes, Enéas Gomes-Filho, Humberto Henrique de Carvalho

{"title":"Unveiling a differential metabolite modulation of sorghum varieties under increasing tunicamycin-induced endoplasmic reticulum stress.","authors":"Francisco Lucas Pacheco Cavalcante, Sávio Justino da Silva, Lineker de Sousa Lopes, Stelamaris de Oliveira Paula-Marinho, Maria Izabel Florindo Guedes, Enéas Gomes-Filho, Humberto Henrique de Carvalho","doi":"10.1007/s12192-023-01382-5","DOIUrl":"10.1007/s12192-023-01382-5","url":null,"abstract":"Plants trigger endoplasmic reticulum (ER) pathways to survive stresses, but the assistance of ER in plant tolerance still needs to be explored. Thus, we selected sensitive and tolerant contrasting abiotic stress sorghum varieties to test if they present a degree of tolerance to ER stress. Accordingly, this work evaluated crescent concentrations of tunicamycin (TM µg mL-1): control (0), lower (0.5), mild (1.5), and higher (2.5) on the initial establishment of sorghum seedlings CSF18 and CSF20. ER stress promoted growth and metabolism reductions, mainly in CSF18, from mild to higher TM. The lowest TM increased SbBiP and SbPDI chaperones, as well as SbbZIP60, and SbbIRE1 gene expressions, but mild and higher TM decreased it. However, CSF20 exhibited higher levels of SbBiP and SbbIRE1 transcripts. It corroborated different metabolic profiles among all TM treatments in CSF18 shoots and similarities between profiles of mild and higher TM in CSF18 roots. Conversely, TM profiles of both shoots and roots of CSF20 overlapped, although it was not complete under low TM treatment. Furthermore, ER stress induced an increase of carbohydrates (dihydroxyacetone in shoots, and cellobiose, maltose, ribose, and sucrose in roots), and organic acids (pyruvic acid in shoots, and butyric and succinic acids in roots) in CSF20, which exhibited a higher degree of ER stress tolerance compared to CSF18 with the root being the most affected plant tissue. Thus, our study provides new insights that may help to understand sorghum tolerance and the ER disturbance as significant contributor for stress adaptation and tolerance engineering.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41101028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of multifunctional immune complement component 1q (C1q) and apoptosis-related genes in Macrophthalmus japonicus tissues and human cells following exposure to environmental pollutants. 多功能免疫补体组分1q（C1q）和凋亡相关基因在日本大蠊组织和人类细胞暴露于环境污染物后的反应。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-10-26 DOI: 10.1007/s12192-023-01389-y

Kiyun Park, Byoung-San Moon, Ihn-Sil Kwak

{"title":"Responses of multifunctional immune complement component 1q (C1q) and apoptosis-related genes in Macrophthalmus japonicus tissues and human cells following exposure to environmental pollutants.","authors":"Kiyun Park, Byoung-San Moon, Ihn-Sil Kwak","doi":"10.1007/s12192-023-01389-y","DOIUrl":"10.1007/s12192-023-01389-y","url":null,"abstract":"Apoptosis is a key defense process for multiple immune system functions, playing a central role in maintaining homeostasis and cell development. The purpose of this study was to evaluate the effects of environmental pollutant exposure on immune-related apoptotic pathways in crab tissues and human cells. To do this, we characterized the multifunctional immune complement component 1q (C1q) gene and analyzed C1q expression in Macrophthalmus japonicus crabs after exposure to di(2-ethylhexyl) phthalate (DEHP) or hexabromocyclododecanes (HBCDs). Moreover, the responses of apoptotic signal-related genes were observed in M. japonicus tissues and human cell lines (HEK293T and HCT116). C1q gene expression was downregulated in the gills and hepatopancreas of M. japonicus after exposure to DEHP or HBCD. Pollutant exposure also increased antioxidant enzyme activities and altered transcription of 15 apoptotic signaling genes in M. japonicus. However, patterns in apoptotic signaling in response to these pollutants differed in human cells. HBCD exposure generated an apoptotic signal (cleaved caspase-3) and inhibited cell growth in both cell lines, whereas DEHP exposure did not produce such a response. These results suggest that exposure to environmental pollutants induced different levels of immune-related apoptosis depending on the cell or tissue type and that this induction of apoptotic signaling may trigger an initiation of carcinogenesis in M. japonicus and in humans as consumers.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50160814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTED ARTICLE: circABCA3 knockdown relieves hypoxia-induced human cardiac microvascular endothelial cell dysfunction by targeting the miR-671-5p/PCSK9 axis. 文章摘要:circABCA3敲低通过靶向miR-671-5p/PCSK9轴缓解缺氧诱导的人心脏微血管内皮细胞功能障碍。

IF 3.3 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-09-07 DOI: 10.1007/s12192-023-01377-2

Hui Hui, Gaowa Zhao, Mingliang Du, Qin Yu

引用次数: 0

Genome-wide analysis of the SPL transcription factor family and its response to water stress in sunflower (Helianthus annuus). 向日葵（Helianthus annuus）中 SPL 转录因子家族的全基因组分析及其对水分胁迫的响应。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 DOI: 10.1007/s12192-023-01388-z

Kundansing R Jadhao, Sonam S Kale, Nilesh S Chavan, Pandharinath H Janjal

{"title":"Genome-wide analysis of the SPL transcription factor family and its response to water stress in sunflower (Helianthus annuus).","authors":"Kundansing R Jadhao, Sonam S Kale, Nilesh S Chavan, Pandharinath H Janjal","doi":"10.1007/s12192-023-01388-z","DOIUrl":"https://doi.org/10.1007/s12192-023-01388-z","url":null,"abstract":"SPL (SQUAMOSA promoter binding proteins-like) are plant-specific transcription factors that play essential roles in a variety of developmental processes as well as the ability to withstand biotic and abiotic stresses. To date, numerous species have been investigated for the SPL gene family, but so far, no SPL family genes have been thoroughly identified and characterized in the sunflower (Helianthus annuus). In this study, 25 SPL genes were identified in the sunflower genome and were unevenly distributed on 11 chromosomes. According to phylogeny analysis, 59 SPL genes from H. annuus, O. sativa, and A. thaliana were clustered into seven groups. Furthermore, the SPL genes in groups-I and II were demonstrated to be potential targets of miR156. Synteny analysis showed that 7 paralogous gene pairs exist in HaSPL genes and 26 orthologous gene pairs exist between sunflower and rice, whereas 21 orthologous gene pairs were found between sunflower and Arabidopsis. Segmental duplication appears to have played a vital role in the expansion processes of sunflower SPL genes, and because of selection pressure, all duplicated genes have undergone purifying selection. Tissue-specific gene expression analysis of the HaSBP genes proved their diverse spatiotemporal expression patterns, which were predominantly expressed in floral organs and differentially expressed in stem, axil, and root tissues. The expression pattern of HaSPL genes under water stress showed broad involvement of HaSPLs in the response to flood and drought stresses. This genome-wide identification investigation provides detailed information on the sunflower SPL transcription factor gene family and establishes a strong platform for future research on sunflower responses to abiotic stress tolerance.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic loss of the HSP70cA gene in the vertebrate lineage. 脊椎动物中 HSP70cA 基因的基因组缺失。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 DOI: 10.1007/s12192-023-01370-9

Alisha Merchant, Bradly I Ramirez, Melinda N Reyes, Dysocheata Van, Marilin Martinez-Colin, Damilola O Ojo, Esmeralda L Mazuca, Heidi J De La O, Abigayle M Glenn, Claudia G Lira, Hashimul Ehsan, Ermeng Yu, Gen Kaneko

{"title":"Genomic loss of the HSP70cA gene in the vertebrate lineage.","authors":"Alisha Merchant, Bradly I Ramirez, Melinda N Reyes, Dysocheata Van, Marilin Martinez-Colin, Damilola O Ojo, Esmeralda L Mazuca, Heidi J De La O, Abigayle M Glenn, Claudia G Lira, Hashimul Ehsan, Ermeng Yu, Gen Kaneko","doi":"10.1007/s12192-023-01370-9","DOIUrl":"https://doi.org/10.1007/s12192-023-01370-9","url":null,"abstract":"Metazoan 70 kDa heat shock protein (HSP70) genes have been classified into four lineages: cytosolic A (HSP70cA), cytosolic B (HSP70cB), endoplasmic reticulum (HSP70er), and mitochondria (HSP70m). Because previous studies have identified no HSP70cA genes in vertebrates, we hypothesized that this gene was lost on the evolutionary path to vertebrates. To test this hypothesis, the present study conducted a comprehensive database search followed by phylogenetic and synteny analyses. HSP70cA genes were found in invertebrates and in two of the three subphyla of Chordata, Cephalochordata (lancelets) and Tunicata (tunicates). However, no HSP70cA gene was found in the genomes of Craniata (another subphylum of Chordata; lamprey, hagfish, elephant shark, and coelacanth), suggesting the loss of the HSP70cA gene in the early period of vertebrate evolution. Synteny analysis using available genomic resources indicated that the synteny around the HSP70 genes was generally conserved between tunicates but was largely different between tunicates and lamprey. These results suggest the presence of dynamic chromosomal rearrangement in early vertebrates that possibly caused the loss of the HSP70cA gene in the vertebrate lineage.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutations F352A and Y528A in human HSP90α reduce fibronectin association and fibrillogenesis in cell-derived matrices. 人类 HSP90α 中的 F352A 和 Y528A 突变会减少纤维粘连蛋白的结合以及细胞衍生基质中的纤维生成。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 DOI: 10.1007/s12192-023-01362-9

Abir Chakraborty, Ronald Tonui, Adrienne Lesley Edkins

{"title":"Mutations F352A and Y528A in human HSP90α reduce fibronectin association and fibrillogenesis in cell-derived matrices.","authors":"Abir Chakraborty, Ronald Tonui, Adrienne Lesley Edkins","doi":"10.1007/s12192-023-01362-9","DOIUrl":"https://doi.org/10.1007/s12192-023-01362-9","url":null,"abstract":"HSP90 is a ubiquitously expressed chaperone protein that regulates the maturation of numerous substrate proteins called 'clients'. The glycoprotein fibronectin (FN) is an important protein of the extracellular matrix (ECM) and a client protein of HSP90. FN and HSP90 interact directly, and the FN ECM is regulated by exogenous HSP90 or HSP90 inhibitors. Here, we extend the analysis of the HSP90 - FN interaction. The importance of the N-terminal 70-kDa fragment of fibronectin (FN70) and FN type I repeat was demonstrated by competition for FN binding between HSP90 and the functional upstream domain (FUD) of the Streptococcus pyogenes F1 adhesin protein. Furthermore, His-HSP90α mutations F352A and Y528A (alone and in combination) reduced the association with full-length FN (FN-FL) and FN70 in vitro. Unlike wild type His-HSP90α, these HSP90 mutants did not enhance FN matrix assembly in the Hs578T cell line model when added exogenously. Interestingly, the HSP90 E353A mutation, which did not significantly reduce the HSP90 - FN interaction in vitro, dramatically blocked FN matrix assembly in Hs578T cell-derived matrices. Taken together, these data extend our understanding of the role of HSP90 in FN fibrillogenesis and suggest that promotion of FN ECM assembly by HSP90 is not solely regulated by the affinity of the direct interaction between HSP90 and FN.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative proteomic analyses uncover regulatory roles of Nrf2 in human endothelial cells. 定量蛋白质组分析揭示了 Nrf2 在人类内皮细胞中的调控作用。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 DOI: 10.1007/s12192-023-01366-5

Karan Naresh Amin, Palanichamy Rajaguru, Takayoshi Suzuki, Koustav Sarkar, Kumar Ganesan, Kunka Mohanram Ramkumar

{"title":"Quantitative proteomic analyses uncover regulatory roles of Nrf2 in human endothelial cells.","authors":"Karan Naresh Amin, Palanichamy Rajaguru, Takayoshi Suzuki, Koustav Sarkar, Kumar Ganesan, Kunka Mohanram Ramkumar","doi":"10.1007/s12192-023-01366-5","DOIUrl":"https://doi.org/10.1007/s12192-023-01366-5","url":null,"abstract":"Nuclear factor erythroid 2-related factor 2 (Nrf2), a transcriptional regulator, is the predominant factor in modulating oxidative stress and other cellular signaling responses. Studies from our lab and others highlighted that activation of the Nrf2 pathway by small molecules improves endothelial function by suppressing oxidative and endoplasmic reticulum (ER) stress. However, the exact mechanisms by which Nrf2 elicits these effects are unknown. In the present study, we developed CRISPR/Cas9-mediated Nrf2 knocked-out human endothelial cells, and proteomic signature was studied using LC-MS/MS. We identified 723 unique proteins, of which 361 proteins were found to be differentially regulated and further screened in the Nrf2ome online database, where we identified a highly interconnected signaling network in which 70 proteins directly interact with Nrf2. These proteins were found to regulate some key cellular and metabolic processes in the regulation actin cytoskeleton, ER stress, angiogenesis, inflammation, Hippo signaling pathway, and epidermal growth factor/fibroblast growth factor (EGF/FGF) signaling pathway. Our findings suggest the role of Nrf2 in maintaining endothelium integrity and its relationship with the crucial cellular processes which help develop novel therapeutics against endothelial dysfunction and its associated complications.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aucubin promotes activation of AMPK and alleviates cerebral ischemia/reperfusion injury in rats. 杜仲甙能促进 AMPK 的激活，缓解大鼠脑缺血再灌注损伤。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-08-22 DOI: 10.1007/s12192-023-01372-7

Jin-Jing Zhao, Bo Zhao, Xiao Bai, Shuang Zhang, Rui Xu

{"title":"Aucubin promotes activation of AMPK and alleviates cerebral ischemia/reperfusion injury in rats.","authors":"Jin-Jing Zhao, Bo Zhao, Xiao Bai, Shuang Zhang, Rui Xu","doi":"10.1007/s12192-023-01372-7","DOIUrl":"10.1007/s12192-023-01372-7","url":null,"abstract":"In the current investigation, we explored the benefits of aucubin against rodent ischemia/reperfusion (I/R) damages in brains and elucidated the role of 5'-AMP-activated protein kinase (AMPK) in its neuroprotective action. I/R model of brain was established in male three-month-old rats through 2 h of middle cerebral artery occlusion followed by two days of reperfusion. Aucubin boosted phosphorylation of AMPKα in ipsilateral cortex of injured rats. Then, rats were exposed to cerebral I/R damage and received treatment of aucubin and compound C (a well-known AMPK inhibitor). It was found that aucubin administration improved neurological symptom score, decreased infarct volume, and mitigated cerebral edema in injured rats. Aucubin administration upregulated Nrf2 expression and abated oxidative stress in ipsilateral cortex of injured rats. Aucubin administration reduced levels of multiple pro-inflammatory cytokines, suppressed microglial activation and neutrophil infiltration, and promoted M2 polarization in injured rats. More importantly, compound C abolished the neuroprotective, anti-oxidant and inflammation-modulating effects of aucubin in injured rats, at least in part. Therefore, we concluded that activation of AMPK by aucubin alleviated I/R injury in brain through abating oxidative stress and suppressing inflammation, identifying a potential candidate for those patients of ischemic stroke.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10108091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of body composition and cardiorespiratory fitness on plasma HSP72, norepinephrine, insulin, and glucose responses to an acute aerobic exercise bout performed in the fed state. 身体成分和心肺功能对血浆 HSP72、去甲肾上腺素、胰岛素和葡萄糖对进食状态下急性有氧运动的反应的影响。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-07-18 DOI: 10.1007/s12192-023-01364-7

Carlos Henrique de Lemos Muller, Cesar Eduardo Jacintho Moritz, Helena Trevisan Schroeder, Ana Maria Oliveira Battastini, Alvaro Reischak-Oliveira, Paulo Ivo Homem de Bittencourt Júnior, Giuseppe De Vito, Maurício Krause

{"title":"Influence of body composition and cardiorespiratory fitness on plasma HSP72, norepinephrine, insulin, and glucose responses to an acute aerobic exercise bout performed in the fed state.","authors":"Carlos Henrique de Lemos Muller, Cesar Eduardo Jacintho Moritz, Helena Trevisan Schroeder, Ana Maria Oliveira Battastini, Alvaro Reischak-Oliveira, Paulo Ivo Homem de Bittencourt Júnior, Giuseppe De Vito, Maurício Krause","doi":"10.1007/s12192-023-01364-7","DOIUrl":"10.1007/s12192-023-01364-7","url":null,"abstract":"Being overweight is already considered a metabolic risk factor, which can be overcome by increasing cardiorespiratory fitness (CRF). Acute exercise is known to induce changes in plasma hormones and heat shock proteins release. However, there is a lack of studies investigating the impact of body composition and CRF on these variables following acute aerobic exercise. To assess the influence of body composition and cardiorespiratory fitness on plasma heat shock protein 72 kDa (HSP72), norepinephrine (NE), insulin, and glucose responses to an acute aerobic exercise bout in the fed state. Twenty-four healthy male adults were recruited and allocated into three groups: overweight sedentary (n = 8), normal weight sedentary (n = 8), and normal weight active (n = 8). The volunteers performed an acute moderate exercise session on a treadmill at 70% of VO2 peak. Blood samples were drawn at baseline, immediately post-exercise, and at 1-h post-exercise. The exercise session did not induce changes in HSP72 nor NE but changes in glucose and insulin were affected by body mass index. Also, subjects with elevated CRF maintain reduced NE through exercise. At baseline, the overweight sedentary group showed elevated NE, insulin, and glucose; these last two impacting the HOMA-IR index. Thirty minutes of aerobic exercise at 70% VO2 peak, in the fed state, did not change the levels of plasma NE and HSP72. Elevated body composition seems to impact metabolic profile and increase sympathetic activity. Conversely, subjects with increased cardiorespiratory fitness seem to have attenuated sympathetic activity.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10204084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-Acetylcysteine may exert hepatoprotective effect by regulating Meteorin-Like levels in Adriamycin-induced liver injury. 在阿霉素诱导的肝损伤中，N-乙酰半胱氨酸可通过调节Meteorin-Like水平发挥保肝作用。

IF 3.8 3区生物学

Cell Stress & Chaperones Pub Date : 2023-11-01 Epub Date: 2023-09-05 DOI: 10.1007/s12192-023-01376-3

Sercan Kaya, Tuba Yalcın, Ahmet Tektemur, Tuncay Kuloğlu

{"title":"N-Acetylcysteine may exert hepatoprotective effect by regulating Meteorin-Like levels in Adriamycin-induced liver injury.","authors":"Sercan Kaya, Tuba Yalcın, Ahmet Tektemur, Tuncay Kuloğlu","doi":"10.1007/s12192-023-01376-3","DOIUrl":"10.1007/s12192-023-01376-3","url":null,"abstract":"Adriamycin (ADR) is an important chemotherapeutic drug, but it has serious side effects such as hepatotoxicity. This study aimed to evaluate whether N-acetylcysteine (NAC) has hepatoprotective effects against ADR-induced hepatotoxicity in rats. In addition, it was aimed to determine how Meteorin-Like (MtrnL), which has pleiotropic effects on immunology, inflammation, and metabolism, is affected by ADR and/or NAC applications in liver tissue. 28 rats were randomly assigned to one of four equal groups in the study: control (no treatment), NAC (150 mg/kg/day of NAC intraperitoneally (i.p), ADR (15 mg/kg only on the first day of the experiment), and ADR + NAC (ADR 15 mg/kg on the first day of the experiment + 150 mg/kg/day NAC i.p). After 15 days, liver enzyme levels in serum, oxidant/antioxidant parameters in liver tissue, histopathological changes, caspase 3 (Casp3) and heat shock protein 70 (HSP-70) immunoreactivities, and MtrnL levels were examined. Histopathological changes, liver enzyme levels, as well as HSP-70, and Casp3 immunoreactivities increased due to ADR application. Additionally, MtrnL levels in liver tissue were significantly increased as a result of ADR application. However, it was detected that the NAC application significantly regulated the ADR-induced changes. Furthermore, it was determined that NAC administration regulated the changes in ADR-induced oxidative stress parameters. We propose that NAC may exert a hepatoprotective effect by regulating ADR-induced altered oxidative stress parameters, MtrnL levels, Casp3, and HSP-70 immunoreactivities in the liver.","PeriodicalId":9684,"journal":{"name":"Cell Stress & Chaperones","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10153024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0