The influence of HSP inducers on salinity stress in sterlet sturgeon (Acipenser ruthenus): In vitro study on HSP expression, immune responses, and antioxidant capacity

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Sevda Zarei , Hossein Ghafouri , Leila Vahdatiraad , Behrooz Heidari
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Abstract

Heat shock proteins (HSPs) play a crucial role in antioxidant systems, immune responses, and enzyme activation during stress conditions. Salinity changes can cause stress and energy expenditure in fish, resulting in mortality, especially in fingerlings. The purpose of this study was to examine the relationship between salinity and HSPs in stressed fish by assessing the effects of various HSP inducers (HSPis), including Pro-Tex® (800 mM), amygdalin (80 mM), and a novel synthetic compound derived from pirano piranazole (80 µM), on isolated cells from Sterlet Sturgeon (Acipenser ruthenus) exposed to 13 ‰ salinity (S13). After liver, kidney, and gill cells were cultured, the HSPi compounds were treated in vitro in the presence and absence of salinity. The expression patterns of HSP27, HSP70, and HSP90 were assessed by Western blotting. Biochemical enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and lactate dehydrogenase), cortisol levels, and immune parameters (component 3, immunoglobulin M, and lysozyme) were measured before and after treatment with HSPis and HSPi + S13. According to these findings, HSPis positively modulate HSP expression, immune responses, and antioxidant levels. Furthermore, they increased in vitro cell survival by maintaining cortisol levels and biochemical enzyme activities in A. ruthenus under saline conditions (P < 0.0001). In conclusion, HSPis can increase A. ruthenus resistance to salinity stress. However, the results also indicated that these compounds can reverse the adverse effects of salinity. The effectiveness of this approach depends on further research into the effects of these ecological factors on the health status of the species, especially in vivo and in combination with other stresses.

Abstract Image

HSP诱导剂对鲟鱼(Acipenser ruthenus)盐度胁迫的影响:关于HSP表达、免疫反应和抗氧化能力的体外研究。
热休克蛋白(HSPs)在应激条件下的抗氧化系统、免疫反应和酶活化中发挥着至关重要的作用。盐度变化会导致鱼类应激和能量消耗,造成死亡,尤其是幼鱼。本研究的目的是通过评估各种 HSP 诱导剂(HSPis),包括 Pro-Tex® (800mM)、杏仁苷 (80mM) 和一种从 pirano piranazole 提取的新型合成化合物 (80µM),对暴露在 13‰ 盐度(S13)下的鲟鱼(Acipenser ruthenus)离体细胞的影响,来研究盐度与应激鱼体内 HSPs 之间的关系。培养肝脏、肾脏和鳃细胞后,在有盐度和无盐度条件下对 HSPi 复合物进行体外处理。用 Western 印迹法评估 HSP27、HSP70 和 HSP90 的表达模式。在使用 HSPis 和 HSPi + S13 处理前后测量了生化酶(AST、ALT、ALP 和 LDH)、皮质醇水平和免疫参数(C3、IgM 和 LYZ)。根据这些研究结果,HSPis 能积极调节 HSP 表达、免疫反应和抗氧化剂水平。此外,在生理盐水条件下,HSPis 还能维持芦竹的皮质醇水平和生化酶活性,从而提高体外细胞存活率(P ˂ 0.0001)。总之,HSPis 能增强芦竹对盐胁迫的抵抗力。不过,研究结果也表明,这些化合物可以逆转盐度的不利影响。这种方法的有效性取决于进一步研究这些生态因素对物种健康状况的影响,特别是在体内以及与其他胁迫结合时的影响。
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来源期刊
Cell Stress & Chaperones
Cell Stress & Chaperones 生物-细胞生物学
CiteScore
7.60
自引率
2.60%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.
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