Cell Biochemistry and Function最新文献

{"title":"Matrix Stiffness Regulates Interleukin-10 Secretion in Human Microglia (HMC3) via YAP-Mediated Mechanotransduction","authors":"Xue Fang,&nbsp;Haiying Jia,&nbsp;Shaoshan Pan,&nbsp;Qian Liu,&nbsp;Qian Wang,&nbsp;Ye Feng,&nbsp;Weiping Ding,&nbsp;Tianzhi Luo","doi":"10.1002/cbf.70061","DOIUrl":"https://doi.org/10.1002/cbf.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Microglia, as resident immune cells in the brain, adhere to the extracellular matrix and typically exhibit anti-inflammatory polarization under normal physiological conditions. Despite their pivotal roles, the regulatory effects of extracellular matrix properties on microglial function and the associated molecular mechanisms remain inadequately understood. Here, we elucidate how matrix stiffness modulates interleukin-10 (IL-10) secretion in human microglia (HMC3) via yes-associated protein (YAP)-mediated mechanotransduction. Using soft collagen Ⅰ-coated hydrogels, we observed a substantial reduction in IL-10 secretion, accompanied by a decrease in the expression and nuclear localization of YAP compared to cells adhered to glass substrates. With increasing hydrogel substrate stiffness, the expression and nuclear localization of YAP were enhanced, leading to an elevated secretion of IL-10. Subsequently, to further investigate the relationship between YAP and IL-10, we performed YAP depletion experiments, which revealed that nuclear exclusion of YAP suppressed IL-10 secretion. Interestingly, overexpression of YAP in microglia did not markedly affect IL-10 levels. We seeded YAP-knockdown microglia onto hydrogels of varying stiffness, and no significant differences were observed in IL-10 secretion. Our findings suggested that cytoskeletal polymerization was crucial for the regulation of IL-10 secretion mediated by YAP. Given the crucial role of IL-10 in the tumor microenvironment, we further found shYAP-microglia attenuated the pro-proliferative effect of microglia on gliomas. Besides, when YAP was silenced, actin of human microglia decreased, and their contractility was weakened. In summary, this study identifies YAP as a pivotal molecule in controlling cytokine secretion and sensing matrix stiffness in microglia. These insights offer potential therapeutic avenues for glioma treatment by targeting YAP-mediated pathways in microglial cells.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 3","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Bio Notes: A New Article Type for Cell Biochemistry and Function","authors":"Raphael Gaudin,&nbsp;Robert J. Heath","doi":"10.1002/cbf.70062","DOIUrl":"https://doi.org/10.1002/cbf.70062","url":null,"abstract":"","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Cyanidin-3-Glucoside on the Modulation of Immune Cell Responses by Mesenchymal Stem Cell-Conditioned Medium","authors":"Sumara de Freitas,&nbsp;Edson Naoto Makiyama,&nbsp;Bruna Roberta Oliveira Neves,&nbsp;Iolanda Silva Rafael Pizzolato Cezar,&nbsp;Carlos Eduardo da Silva Gonçalves,&nbsp;Marcelo Macedo Rogero,&nbsp;Ricardo Ambrósio Fock","doi":"10.1002/cbf.70059","DOIUrl":"https://doi.org/10.1002/cbf.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Mesenchymal stem cells (MSCs) are emerging as promising therapeutic agents due to their immunomodulatory effects, primarily mediated via paracrine signaling. Similarly, anthocyanins, such as cyanidin-3-glucoside (C3G), have demonstrated significant anti-inflammatory properties. In this context, this study investigated the immunomodulatory potential of C3G on MSCs, and subsequent effects on macrophage and lymphocyte responses. Cytotoxicity assays identified 50 µM as the highest nontoxic C3G concentration for MSCs. Flow cytometry confirmed that C3G treatment did not affect MSC viability or cell cycle distribution, even under LPS stimulation. Cytokine production by MSCs was evaluated after treatment with C3G and LPS. While no significant changes were observed in IL-6, IL-10, TGF-β, or PGE₂ levels, IL-1β production was significantly reduced in LPS-stimulated MSCs treated with C3G. Protein expression analysis revealed decreased NFκB phosphorylation in LPS-stimulated MSCs treated with C3G, with no changes detected in STAT-3 or PCNA expression. The immunomodulatory effects of MSC-derived conditioned media on macrophages and lymphocytes were also assessed. In LPS-stimulated macrophages, conditioned media from MSCs reduced the production of IL-1β, IL-6, and IL-12. Interestingly, conditioned media from C3G-treated MSCs specifically decreased TNF-α levels, enhanced IL-10 secretion, and further inhibited NFκB phosphorylation. In LPS-stimulated lymphocytes, conditioned media from C3G-treated MSCs suppressed IL-2 production while increasing IL-10 levels. In summary, these findings demonstrate that conditioned media from C3G-treated MSCs modulates immune cell responses more effectively than C3G alone. C3G influences the paracrine activity of MSCs, resulting in a shift in the secretory profile and subsequent effects on immune cell behavior.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Protective Potential of Crocin in Septic Acute Liver Injury via Assessment of TLR4/HGM1/NF-κB Signaling Pathway, Oxidative Stress and Heat Shock Response","authors":"Ali Tugrul Akin,&nbsp;Emin Kaymak,&nbsp;Tayfun Ceylan,&nbsp;Nurhan Kuloglu,&nbsp;Derya Karabulut,&nbsp;Ayse Toluk","doi":"10.1002/cbf.70058","DOIUrl":"https://doi.org/10.1002/cbf.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>Sepsis, defined as a systemic inflammatory response, is one of the conditions with the highest mortality rates. Crosin (CRO) is one of the active ingredients in saffron and known for its various pharmacological effects. It has been reported to have protective and healing effects on liver tissue. In this study, we aimed to investigate the protective role of CRO in lipopolysaccharide (LPS)-induced acute liver injury. 40 male Wistar Albino rats aged 8–12 weeks were randomly divided into 4 groups: Control, CRO (50 mg/kg, intraperitoneal administration for 9 days), LPS (30 mg/kg, single dose), and LPS + CRO (50 mg/kg CRO for 9 days along with a single dose of 30 mg/kg LPS). Following the experimental procedure, liver and blood samples were collected for further analyses. Histopathological analysis revealed a marked increase in liver damage in the LPS group, as evidenced by significant histopathological changes. In contrast, the liver histology in the LPS + CRO group closely resembled that of the Control and CRO groups, exhibiting substantially less damage compared to the LPS group. Immunohistochemical examinations showed a significant increase in the expressions of TLR4, HMGB1, NF-κB, TNF- α, HSP70, and HSP90 in the LPS group. However, in the LPS + CRO group, the levels of these markers were significantly lower compared to the LPS group. ELISA analyses showed a significant increase in MDA, IL-6, and TGF-β and a decrease in SOD, CAT, GSH levels in the LPS group. Conversely, in the LPS + CRO group, CRO applications exhibited a significant protective effect on these alterations. Additionally, AST, ALT, and LDH levels were significantly elevated in the LPS group, while albumin levels were lower in the LPS group. CRO applications in the LPS + CRO group were observed to have a protective effect on these parameters. We believe that CRO holds significant potential in the treatment of acute inflammatory diseases such as septic acute liver injury and should not be overlooked.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Myeloid Leukemia-Osteoblast Interaction Mediated Autophagy Induction Protects Against Cytarabine Induced Apoptosis","authors":"Kamini Shivhare,&nbsp;Neeraj Kumar Satija","doi":"10.1002/cbf.70055","DOIUrl":"https://doi.org/10.1002/cbf.70055","url":null,"abstract":"<div>\u0000 \u0000 <p>High rate of relapse, following chemotherapy, in acute myeloid leukemia (AML) is a major concern. The chemoprotection conferred by the bone marrow microenvironment has lately been recognized, in addition to autophagy-mediated chemoresistance. Thus, the present study explored the effect of osteoblast on autophagy in AML and its impact on sensitivity to cytarabine (Ara-C) in the context of endosteal niche. Co-culture of KG1-a, HL60, or THP-1 AML cells with osteoblastic Saos-2 cell line induced autophagy in AML cell lines under direct contact. HL60 cells when co-culture with Saos-2 demonstrated more resistance to Ara-C induced apoptosis, which was reversed upon chloroquine treatment. Similarly, inhibition of autophagy in AML cell by knocking down Beclin-1 enhanced HL60 sensitivity to Ara-C. An interesting observation was upregulation of autophagy even in Saos-2 cells upon co-culture with AML cell, and increase in HL60 apoptosis in response to Ara-C on Beclin-1 knockdown in osteoblast cell. This highlights that autophagy plays a chemoprotective role in the endosteal niche in AML against Ara-C.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction to “LINC00240 Promotes Gastric Cancer Cell Proliferation, Migration and EMT via the MiR-124-3p/DNMT3B Axis”","authors":"","doi":"10.1002/cbf.70054","DOIUrl":"https://doi.org/10.1002/cbf.70054","url":null,"abstract":"<p>Y. Li, J. Yan, Y. Wang, C. Wang, C. Zhang, and G. Li, “LINC00240 Promotes Gastric Cancer Cell Proliferation, Migration and EMT via the MiR-124-3p/DNMT3B Axis,” <i>Cell Biochemistry and Function</i> 38, no. 8 (2020): 1079–1188, https://doi.org/10.1002/cbf.3551.</p><p>The above article, published online on 11 June 2020 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editors-in-Chief, Raphael Gaudin and Robert Heath; and John Wiley &amp; Sons Ltd. An investigation by the publisher revealed several flaws and inconsistencies between the results presented and the experimental methods described. Furthermore, the article reports major results in the cell lines BGC-823 and SGC-7901, reported as contaminated [1, 2]. Accordingly, the article is retracted as the editors consider the conclusions of this article to be invalid.</p><p><b>References</b></p><p>[1] F. Ye, C. Chen, J. Qin, J. Liu, and C. Zheng, “Genetic Profiling Reveals an Alarming Rate of Cross-Contamination Among Human Cell Lines Used in China,” <i>FASEB Journal</i> 29, no. 10 (2015): 4268–4272, https://doi.org/10.1096/fj.14-266718.</p><p>[2] X. Bian, Z. Yang, H. Feng, H. Sun, and Y. Liu, “A Combination of Species Identification and STR Profiling Identifies Cross-Contaminated Cells From 482 Human Tumor Cell Lines,” <i>Scientific Reports</i> 7, no. 1 (2017): 9774, https://doi.org/10.1038/s41598-017-09660-w.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbf.70054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Carvedilol Against Oxidative Stress Induced by Palmitic Acid in Primary Rat Hepatocytes","authors":"Sandra A. Serna Salas,&nbsp;Turtushikh Damba,&nbsp;Manon Buist-Homan,&nbsp;Han Moshage","doi":"10.1002/cbf.70057","DOIUrl":"https://doi.org/10.1002/cbf.70057","url":null,"abstract":"<p>Hepatocyte lipotoxicity (HL) is an important factor in the pathogenesis of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). It is defined as the detrimental effects of exposure to (excessive) amounts of toxic lipid species, leading to increased mitochondrial β-oxidation, oxidative stress (OxS), and organellar dysfunction. Carvedilol (CV) is a β-adrenergic blocker with antioxidant properties. To elucidate whether CV protects hepatocytes against lipotoxicity induced by palmitic acid (PA) by reducing OxS and endoplasmic reticulum (ER) stress. Primary rat hepatocytes (rHep) were used. Lipotoxicity was induced by PA (1 mmol/L). Cell damage was evaluated by Sytox Green staining. Mitochondrial generation of reactive oxygen species (mROS) was assessed by MitoSox. mRNA and protein expression were measured by qPCR and Western blot, respectively. Lipid accumulation was measured by Oil Red O staining and triglyceride (TG) content. PA induced cell death in &gt; 80% of cells and increased mROS generation. PA increased mRNA expression of ER stress markers CHOP and sXBP1 and slightly increased lipid accumulation. Expression of the β-oxidation-related gene Cpt1a was increased. CV (10 µmol/L) significantly reduced PA-induced cell death to control levels (&lt; 8% of total cells), and mROS generation and expression of the mitochondrial antioxidant enzymes Sod2 and Cat were increased by 40% by CV in the presence of PA. CV did not change the expression of ER stress markers. CV, added before PA, protects rHep against PA-induced cytotoxicity by reducing OxS and increasing the expression of antioxidant enzymes without any additional protective effect on ER stress or lipid accumulation.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbf.70057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigallocatechin-Gallate: Unraveling Its Protective Mechanisms and Therapeutic Potential","authors":"Xiang-Wen Dong,&nbsp;Wen-Lan Fang,&nbsp;Yun-Hang Li,&nbsp;Yu-Rong Chai","doi":"10.1002/cbf.70056","DOIUrl":"https://doi.org/10.1002/cbf.70056","url":null,"abstract":"<div>\u0000 \u0000 <p>Epigallocatechin-gallate (EGCG), the predominant catechin in green tea, is a key constituent of tea polyphenols. Due to the EGCG's diverse biological activities of anti-inflammatory, antioxidant, and so forth, green tea is believed to exert a positive influence on a variety of diseases. And extensive research had uncovered a range of protective effects attributed to EGCG, indicating its potential to mitigate various pathological conditions. The precise mechanisms through which EGCG operates remain a subject of ongoing discussion among researchers. Reactive oxygen species (ROS), a primary culprit in oxidative stress, have been demonstrated to be reduced by EGCG. Furthermore, nuclear factor kappa-B (NF-κB), a pivotal signal molecular of inflammation progress, has been observed to be suppressed by EGCG. Sirtuins1 (Sirt1) is a histone deacetylase, the obligate substrate of which is NAD+. Evidence suggests that EGCG can enhance the activities of Sirt1 to induce autophagy to protect inflammation injury and oxidative stress in tissues and organs. Despite the promising protective effects of EGCG, its clinical use is constrained by its limited bioavailability. This review aims to consolidate the existing evidence and elucidate the mechanisms that support EGCG's protective role, as well as to explore the challenges and potential strategies for its clinical application.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Microbiota-Derived Metabolites in Prostate Cancer Inflammation and Progression","authors":"Pradeep Kumar,&nbsp;Anil Kumar,&nbsp;Virendra Kumar","doi":"10.1002/cbf.70050","DOIUrl":"10.1002/cbf.70050","url":null,"abstract":"<div>\u0000 \u0000 <p>Prostate cancer (PCa) is the most commonly detected malignancy in men worldwide. PCa is a slow-growing cancer with the absence of symptoms at early stages. The pathogenesis has not been entirely understood including the key risk factors related to PCa development like diet and microbiota derived metabolites. Microbiota may influence the host's immunological responses, inflammatory responses, and metabolic pathways, which may be crucial for the development and metastasis. Similarly, short-chain fatty acids, methylamines, hippurate, bile acids, and other metabolites generated by microbiota may have potential roles in cancer inflammation and progression of cancer. Most studies have focused on the role of metabolites and their pathways involved in chronic inflammation, tumor initiation, proliferation, and progression. In summary, the review discusses the role of microbiota and microbial-derived metabolite-built strategies in inflammation and progression of the PCa.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super-Enhancer Protects Cells From Toxicity of C9orf72 Poly(proline–arginine) by Inducing the Expression of KPNA2/KPNB1","authors":"Miaomiao Chen,&nbsp;Henglu Cui,&nbsp;Xiaoyu Zhang,&nbsp;Shuyan Ma,&nbsp;Jinjing Guo,&nbsp;Zhaoxiu Liu,&nbsp;Donghua Gu,&nbsp;Yihui Fan","doi":"10.1002/cbf.70053","DOIUrl":"10.1002/cbf.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>Hexanucleotide repeat expansions in C9orf72 are the most common genetic mutation associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) (C9-ALS/FTD). Dipeptide repeat (DPR) proteins, such as poly(proline–arginine) (polyPR) generated from G4C2 repeat expansions, have been shown to be highly toxic. In this study, PR20 was labeled with fluorescein isothiocyanate (FITC) to track its cellular localization. Several cell lines demonstrated survival under PR20 treatment by sequestering PR20 in the cytoplasm. Treatment with JQ-1 or Ivermectin (Iver) translocated PR20 into the nucleus, leading to cell death. Mechanistically, KPNA2/KPNB1 interacted with PR20 in the cytoplasm and hindered PR20 from entering the cell nucleus. Genetic silencing of KPNA2/KPNB1 converted PR20-resistant cells into PR20-sensitive cells. Treatment with JQ1 significantly reduced the protein levels of KPNA2/KPNB1, allowing PR20 to enter the nucleus. Overexpression of KPNA2 or KPNB1 effectively blocked cell death induced by co-treatment with JQ-1 and PR20. Our results indicate that super-enhancers shield cells from PR20 toxicity by upregulating the expression of KPNA2/KPNB1.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}