Cell Biochemistry and Function最新文献

Proteomics Profiling of Early Coronary Artery Injury in a Rat Model of Type 1 Diabetes Mellitus 1型糖尿病大鼠早期冠状动脉损伤的蛋白质组学分析

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-28 DOI: 10.1002/cbf.70106

Min Jin, Wenda Wu, Xiaomin Yang, Xiaowan Shi, Xinfang Cao, Shouyuan Tian, Yu Liu

{"title":"Proteomics Profiling of Early Coronary Artery Injury in a Rat Model of Type 1 Diabetes Mellitus","authors":"Min Jin, Wenda Wu, Xiaomin Yang, Xiaowan Shi, Xinfang Cao, Shouyuan Tian, Yu Liu","doi":"10.1002/cbf.70106","DOIUrl":"https://doi.org/10.1002/cbf.70106","url":null,"abstract":"<div>\u0000 \u0000 <p>Individuals with type 1 diabetes mellitus (T1DM) experience an increased risk of cardiovascular disease (CVD). To improve early detection and prevention strategies, a better understanding of early vascular changes is needed. Although coronary artery (CA) damage is a known T1DM complication, its underlying proteomic basis remains unclear. This study used a proteomic approach to identify differentially expressed proteins in the CAs of T1DM rat models, with the goal of identifying novel proteins and pathways associated with early diagnosis and prevention of CA complications. We established a streptozotocin-induced T1DM model in male Sprague–Dawley rats and conducted tandem mass tag-based quantitative proteomics and bioinformatics analyses to investigate protein expression profiles in CAs. The analyses identified 443 differentially expressed proteins, with 229 upregulated and 214 downregulated proteins. Functional annotation and pathway enrichment analyses revealed that these proteins primarily participate in lipid metabolism, the peroxisome proliferator-activated receptor (PPAR) signaling pathway, peroxisome function, and butanoate metabolism. Validation experiments using Western blotting analysis and quantitative real-time PCR confirmed significant upregulation of 3-hydroxy-3-methylglutaryl coenzyme A synthase 2 (HMGCS2), fatty acid-binding protein 4 (FABP4), and platelet glycoprotein 4 (CD36) at the protein and mRNA levels in diabetic rat CAs, consistent with the proteomic results. Our findings indicate that HMGCS2, FABP4, and CD36 may serve as important molecular markers for the early diagnosis or therapeutic targeting of CA damage in T1DM. The observed molecular changes appear to be linked to the PPAR signaling pathway.</p>\u0000 <p><b>Clinical trial registration</b>. Not applicable.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 8","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role and Research Progress of Mitochondria in Long-Term Cold-Stored Platelets 线粒体在长期冷藏血小板中的作用及研究进展

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-23 DOI: 10.1002/cbf.70104

Yujiao Chen, Shadamu Yusuying, Dongjiang Xu, Wenxiang Cheng

{"title":"The Role and Research Progress of Mitochondria in Long-Term Cold-Stored Platelets","authors":"Yujiao Chen, Shadamu Yusuying, Dongjiang Xu, Wenxiang Cheng","doi":"10.1002/cbf.70104","DOIUrl":"https://doi.org/10.1002/cbf.70104","url":null,"abstract":"<div>\u0000 \u0000 <p>Platelets, crucial components of blood, play a vital role in hemostasis and clinical treatments. However, current platelet storage methods at 22 ± 2°C face significant limitations, including a short shelf life (5–7 days), high risk of bacterial contamination, and progressive accumulation of metabolites such as lactic acid during prolonged storage. These issues impair transfusion efficacy and severely restrict the clinical utility of platelets. In contrast, cold storage at 4°C offers potential advantages: it extends platelet shelf life, reduces bacterial contamination risks, minimizes metabolite accumulation, and better preserves platelet hemostatic function. Mitochondria, as essential organelles in platelets, are central to energy metabolism, activation, adenosine triphosphate production, and regulation of cellular processes. They are of great significance for maintaining platelet physiological function and biological activity. This review examines the role of mitochondria in refrigerated platelet storage, focusing on their impact on energy metabolism, apoptosis, and post-transfusion clearance. Additionally, we discuss mitochondrial-targeted intervention strategies and future research directions to optimize platelet storage methods and enhance clinical transfusion outcomes.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Mitochondria-Associated ER in Apoptosis 线粒体相关内质网在细胞凋亡中的作用

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-22 DOI: 10.1002/cbf.70105

Mudan Sang, Xindong Li, Mi Chen, Xiaoli Ren, Sheng Kang, Zhenyu Chang, Qingxia Wu

{"title":"Role of Mitochondria-Associated ER in Apoptosis","authors":"Mudan Sang, Xindong Li, Mi Chen, Xiaoli Ren, Sheng Kang, Zhenyu Chang, Qingxia Wu","doi":"10.1002/cbf.70105","DOIUrl":"https://doi.org/10.1002/cbf.70105","url":null,"abstract":"<div>\u0000 \u0000 <p>Apoptosis represents a critical noninflammatory mechanism for cell clearance in both physiological and pathological contexts, precisely regulated through the balance between proapoptotic and antiapoptotic signaling. Three well-characterized apoptotic pathways have been identified: (1) the intrinsic (mitochondria-mediated) pathway, (2) the extrinsic (death receptor-mediated) pathway, and (3) the endoplasmic reticulum (ER)-stress pathway. These processes are coordinated through the mitochondria-associated ER membrane (MAMs), which serves as a vital coupling platform between mitochondria and the ER. MAMs play pivotal roles in maintaining Ca²⁺ homeostasis and regulating apoptosis through dynamic alterations in architecture (e.g., gap width, contact number) that influence Ca²⁺ trafficking and tethering protein expression. Key protein complexes localized at MAMs (including the IP3Rs-Grp75-VDAC1 complex, Mfn1/Mfn2 complex, and PTPIP51-containing complex) regulate apoptosis through three primary mechanisms: Ca²⁺ homeostasis maintenance, lipid synthesis and transport, and mitochondrial morphology and dynamics. Furthermore, MAMs-mediated mitochondrial dynamics, particularly mitochondrial fission and cristae remodeling, contribute to apoptosis by facilitating Bax/Drp1 dimerization. This review systematically examines: how MAMs' structural dynamics influence Ca²⁺ signaling and tethering protein expression, the roles of MAMs-tethered proteins and their regulators in Ca²⁺ homeostasis, lipid metabolism, and mitochondrial dynamics, and the impact of mitochondrial dynamics on Bax/Drp1 dimerization during apoptosis.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cordycepin Inhibits Proliferation, Migration, and Promotes Apoptosis in Fibrosarcoma HT1080 Cells by Targeting Akt1 and Kinase Activity Through Network Pharmacology Analysis 通过网络药理学分析虫草素通过靶向Akt1和激酶活性抑制纤维肉瘤HT1080细胞的增殖、迁移和促进细胞凋亡

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-22 DOI: 10.1002/cbf.70103

Xin Qiu, Chenyang Li, Jing Wang, Hongyou Yu, Renjun Wang, Liang Wang, Shiqi Zhang, Yu Tang, Zihui Li, Qian Li

{"title":"Cordycepin Inhibits Proliferation, Migration, and Promotes Apoptosis in Fibrosarcoma HT1080 Cells by Targeting Akt1 and Kinase Activity Through Network Pharmacology Analysis","authors":"Xin Qiu, Chenyang Li, Jing Wang, Hongyou Yu, Renjun Wang, Liang Wang, Shiqi Zhang, Yu Tang, Zihui Li, Qian Li","doi":"10.1002/cbf.70103","DOIUrl":"https://doi.org/10.1002/cbf.70103","url":null,"abstract":"<div>\u0000 \u0000 <p>Fibrosarcoma cells exhibit low sensitivity to chemotherapy and significant drug resistance, emphasizing the urgent need for effective, low-toxicity therapeutic agents with reliable production methods and novel treatment strategies. Cordycepin (3′-deoxyadenosine) has shown promising therapeutic potential in cancer treatment. In this study, cordycepin was produced using a genetically engineered <i>Pichia pastoris</i> strain cultured in an inorganic salt medium and purified to over 98% purity via macroporous resin chromatography, providing a cost-effective production alternative. The effects of cordycepin on the human fibrosarcoma cell line HT1080 were assessed using microscopic examination, scratch assays, CCK-8 assays, and flow cytometry (Annexin V-FITC/PI staining). The results demonstrated that cordycepin significantly inhibited cell activity at an effective concentration of 100 μmol/L. Key observations included changes in cell morphology, reduced migration, inhibited proliferation, cell cycle arrest at the G0/G1 and G2/M phases, and induction of apoptosis. Network pharmacology analysis identified 31 potential targets of cordycepin in fibrosarcoma, with its effects on Akt1 (protein kinase B) and disruption of protein phosphorylation pathways emerging as key mechanisms underlying its therapeutic efficacy. Western blot analysis further confirmed that cordycepin simultaneously downregulated both the expression and phosphorylation levels of Akt in a dose-dependent manner.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary Cilia: Hub of Cell Signal Transduction and Emerging Role in Digestive System Tumorigenesis 初级纤毛：细胞信号转导中枢及在消化系统肿瘤发生中的新作用

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-15 DOI: 10.1002/cbf.70102

Wenyan Song, Mingshuo Wang, Siming Kong, Yakun Liu, Xinlong Yan, Hui Bai, Yunfang Wang

{"title":"Primary Cilia: Hub of Cell Signal Transduction and Emerging Role in Digestive System Tumorigenesis","authors":"Wenyan Song, Mingshuo Wang, Siming Kong, Yakun Liu, Xinlong Yan, Hui Bai, Yunfang Wang","doi":"10.1002/cbf.70102","DOIUrl":"https://doi.org/10.1002/cbf.70102","url":null,"abstract":"<div>\u0000 \u0000 <p>Primary cilia, microtubule-based sensory organelles that protrude from the cell surface, are integral to sensing the cellular microenvironment and mediating intercellular signal transduction. They play a crucial role in maintaining homeostasis of major human organs. While primary cilia have been extensively characterized in other organs and linked to tumorigenesis, their specific roles within the digestive system—particularly their pathological loss in digestive cancers—remain poorly defined. Emerging evidence now reveals that cell type-specific ciliary loss (e.g., in cholangiocytes, pancreatic ductal epithelial cells, and gastrointestinal stromal cells and fibroblasts) disrupts critical signaling homeostasis, driving malignant transformation in disorders such as cholangiocarcinoma, chronic pancreatitis, pancreatic ductal adenocarcinoma, and colon cancer. This review synthesizes how ciliary dysfunction in digestive cell subtypes alters key oncogenic pathways (e.g., Hedgehog, Wnt/β-catenin, and PDGFRα signaling) to accelerate tumor initiation and progression. We further explore the therapeutic potential of pharmacologically restoring ciliary integrity to reverse pathway dysregulation, proposing cilia-targeted strategies for early diagnosis and intervention in inflammation-associated digestive malignancies.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant Ostreolysin Promotes the Browning of Preadipocytes by Inhibiting the Expression of Genes Associated With the Hedgehog Signaling Pathway in db/db Mice 重组脂溶素通过抑制db/db小鼠Hedgehog信号通路相关基因的表达促进前脂肪细胞褐变

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-08 DOI: 10.1002/cbf.70099

Fangbing Qi, Beilei Guo, Hui Lu, Yongpeng Lei, Mingyang Liu, Jiali Yan, Jianwei Liu, Sen Liu, Suzhen Li, Qiong Gu, Hua Chao, Yuntao Zhang, Jian Wang

{"title":"Recombinant Ostreolysin Promotes the Browning of Preadipocytes by Inhibiting the Expression of Genes Associated With the Hedgehog Signaling Pathway in db/db Mice","authors":"Fangbing Qi, Beilei Guo, Hui Lu, Yongpeng Lei, Mingyang Liu, Jiali Yan, Jianwei Liu, Sen Liu, Suzhen Li, Qiong Gu, Hua Chao, Yuntao Zhang, Jian Wang","doi":"10.1002/cbf.70099","DOIUrl":"https://doi.org/10.1002/cbf.70099","url":null,"abstract":"<div>\u0000 \u0000 <p>Nonalcoholic steatohepatitis (NASH), a highly prevalent metabolic-related fatty liver disease, has become a major global health issue with limited therapeutic options. Recent studies indicated that ostreolysin (Oly), a protein derived from oyster mushrooms, could be a potential therapeutic agent for NASH. In this study, recombinant Oly (rOly) was expressed in <i>E. coli</i> BL21 (DE3) and purified using Q Sepharose and TOYOPEARL chromatography, with its identity confirmed by SDS-PAGE and mass spectrometry. In<i>db/db</i> mice, subcutaneous injection of rOly at 0.5 and 1 mg/kg every 2 days for 30 days significantly reduced body weight by 14.1% in the high-dose group (<i>p</i> < 0.01), improved insulin resistance (insulin resistance index decreased by 35%, <i>p</i> < 0.05), and alleviated hepatic steatosis as shown by HE and Oil Red O staining.<i>In vitro</i>, rOly induced browning of 3T3-L1 preadipocytes, evidenced by 1.8-fold upregulation of UCP1 (<i>p</i> < 0.05) and 2.3-fold upregulation of ATGL (<i>p</i> < 0.01). Mechanistic studies revealed that rOly inhibited Hedgehog signaling pathway genes <i>Gli1</i> and <i>Ptch1</i> by 70% and 65% (<i>p</i> < 0.0001), respectively, promoting beige adipocyte differentiation. These findings demonstrate that rOly enhances energy metabolism by promoting preadipocyte browning via Hedgehog pathway inhibition, providing a promising basis for treating obesity and metabolic diseases.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persistent Renal Oxidative Stress Despite Mannitol Nephroprotection: The Impact of Social-Single Prolonged Stress in Male and Female Rats Exposed to Cisplatin 尽管甘露醇有肾保护作用，但持续的肾氧化应激：暴露于顺铂的雄性和雌性大鼠的社会单一长期应激的影响

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-08 DOI: 10.1002/cbf.70101

Juliano Ten Kathen Jung, Isabella Pregardier Klann, Bruna Cruz Weber Fulco, Vanessa Angonesi Zborowski, Gilson Zeni, Cristina Wayne Nogueira

{"title":"Persistent Renal Oxidative Stress Despite Mannitol Nephroprotection: The Impact of Social-Single Prolonged Stress in Male and Female Rats Exposed to Cisplatin","authors":"Juliano Ten Kathen Jung, Isabella Pregardier Klann, Bruna Cruz Weber Fulco, Vanessa Angonesi Zborowski, Gilson Zeni, Cristina Wayne Nogueira","doi":"10.1002/cbf.70101","DOIUrl":"https://doi.org/10.1002/cbf.70101","url":null,"abstract":"<p>Cisplatin (CIS) is a chemotherapeutic agent known for nephrotoxicity through oxidative stress. Cancer treatment is also associated with psychological stress. Repeated exposure to social-single prolonged stress (social-SPS) modulates long-term renal oxidative damage and apoptosis in a sex-dependent manner in rats treated with cisplatin (CIS), despite mannitol's nephroprotective effects. We investigated whether repeated exposure to social-single prolonged stress (social-SPS) modulates long-term renal oxidative damage and apoptosis in male and female rats treated with CIS and mannitol. Male and female Wistar rats were divided into three groups: control, CIS + mannitol, and CIS + mannitol + social-SPS. Mannitol was administered 1 h before CIS (2 mg/kg/day, i.p., for 5 days). Social-SPS was applied at three time points. At postnatal day 68, blood and kidney samples were collected for biochemical and Western blot analyses. Plasma renal biomarkers remained unchanged across groups. However, social-SPS increased renal lipid peroxidation (TBARS) and protein oxidation (carbonyl content) in both sexes. CIS+social-SPS decreased catalase activity and altered SOD, GST, and NPSH in a sex-dependent manner. Only female rats showed increased renal BAX/Bcl2 ratio, indicating apoptosis. In males, Na⁺/K⁺-K-ATPase activity correlated positively with NPSH content. Despite mannitol nephroprotection, social stress exacerbated renal oxidative stress. Female rats were more susceptible to apoptosis, suggesting sex-specific vulnerability to combined CIS and stress exposure. These findings highlight the importance of considering psychological stress and sex as modulators of chemotherapeutic toxicity and may inform future strategies for personalized cancer care.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbf.70101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smilax glabra Roxb. Meliorates Hyperuricemia via Regulating Renal Urate Transporter and Remodelulating Intestinal Microbiota in Mice 菝葜。通过调节肾尿酸转运蛋白和重塑肠道微生物群改善小鼠高尿酸血症

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-07 DOI: 10.1002/cbf.70100

Jie Xiao, Weiwei He, Xiaoyang Fang, Rongrong Zhou, Ao Huang, Yikun Wang, Qing Du, Linben Xu, Fei Cheng, Hongliang Zeng

{"title":"Smilax glabra Roxb. Meliorates Hyperuricemia via Regulating Renal Urate Transporter and Remodelulating Intestinal Microbiota in Mice","authors":"Jie Xiao, Weiwei He, Xiaoyang Fang, Rongrong Zhou, Ao Huang, Yikun Wang, Qing Du, Linben Xu, Fei Cheng, Hongliang Zeng","doi":"10.1002/cbf.70100","DOIUrl":"https://doi.org/10.1002/cbf.70100","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Smilax glabra</i> Roxb. is a natural herb, exhibits significant uric acid lowering effect in clinical. However, the specific mechanism remains to be clarified. To study this problem, intraperitoneal injection of Potassium oxazinate (PO) and Hypoxanthine (HX) induced hyperuricemia in mice model, and mice were divided into control, model, and model plus ethanol extract (TFL), ethyl acetate extract (TFL_Y), n-butanol extract (TFL_Z), and residual aqueous extract (TFL_S) of <i>Smilax glabra</i> Roxb., And then a follow-up test. Furthermore, possible pharmacological components were detected by UPLC-Q-TOF-MS. The results showed that serum creatinine, blood urea nitrogen, <span>d</span>-lactate, lipopolysaccharide levels and xanthine oxidase. They could upregulate renal OAT1 and ABCG2, downregulate renal URAT1, reduce renal tubular dilation and interstitial inflammatory cell infiltration. Moreover, they could upregulate intestinal tight junction proteins, increase the number of intestinal goblet cells, and repair damage to the intestinal barrier. Moreover, 16S rRNA sequencing analysis showed that restored intestinal microbiota by increasing probiotic bacteria (<i>Candidatus Saccharimonas</i>, <i>Lachnospiraceae NK4A136_group</i>) and reducing pathogenic bacteria (<i>Helicobacter</i>). Furthermore, it was found that all control taint flavonoids such as Neoastalbin, Astibin, Neoiosastilbin, Isoastalbin, Engeletin, and Isoengeletin.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase of Intracellular Zinc Levels Rather Than Zinc Influx Inhibits Interleukin-2 Production in Zinc Supplemented Jurkat Cells 细胞内锌水平的增加而非锌内流抑制补锌Jurkat细胞中白细胞介素-2的产生

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-07-02 DOI: 10.1002/cbf.70098

Christian M. Sobernig, Henrike J. Fischer, Lothar Rink, Jana Jakobs

{"title":"Increase of Intracellular Zinc Levels Rather Than Zinc Influx Inhibits Interleukin-2 Production in Zinc Supplemented Jurkat Cells","authors":"Christian M. Sobernig, Henrike J. Fischer, Lothar Rink, Jana Jakobs","doi":"10.1002/cbf.70098","DOIUrl":"https://doi.org/10.1002/cbf.70098","url":null,"abstract":"<p>The essential trace element zinc is a well-known modulator of T cell activation. There have been contradictory findings for the impact of zinc supplementation on T cell activation. In our study, we aimed to analyze IL-2 production in Jurkat T cells during zinc supplementation in response to different stimuli. We found that zinc strongly suppresses IL-2 production in Jurkat cells stimulated with phorbol 12-myristate 13-acetate (PMA)/calcimycin or phytohemagglutinin (PHA)/calcimycin. In contrast, zinc had no impact on IL-2 production after PHA stimulation alone, suggesting the inhibitory zinc-effect was linked to high calcium influx. To distinguish if the observed IL-2 suppression is due to either potential competing effects of zinc influx or simple elevation of intracellular zinc levels, we pretreated the Jurkat cells with the zinc ionophore pyrithione for an increase of intracellular zinc before the stimulation. It was sufficient to suppress IL-2 expression even when the cells were not further supplemented with zinc during stimulation. We propose that zinc's inhibitory effects on phosphatases stabilize the phosphorylated NFAT and thus block IL-2 expression. Our findings underline the importance of a balanced zinc status for proper immune functions and suggest a supporting effect of zinc during immunosuppressive treatments.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbf.70098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic and Therapeutic Value of Metabolism-Related Genes in Nephroblastoma: A Focus on the Key Gene NNMT and Its Regulative Effect on Metabolism 肾母细胞瘤代谢相关基因的预后和治疗价值：重点研究关键基因NNMT及其对代谢的调节作用

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-27 DOI: 10.1002/cbf.70097

Chen Ding, Fan Huang, Hongjie Gao, Wan Zhang, Zhiyi Lu, Liting Zhang, Bowen Zhang, Ding Li, YingYing Li, Dan Bi, Fengyin Sun

{"title":"Prognostic and Therapeutic Value of Metabolism-Related Genes in Nephroblastoma: A Focus on the Key Gene NNMT and Its Regulative Effect on Metabolism","authors":"Chen Ding, Fan Huang, Hongjie Gao, Wan Zhang, Zhiyi Lu, Liting Zhang, Bowen Zhang, Ding Li, YingYing Li, Dan Bi, Fengyin Sun","doi":"10.1002/cbf.70097","DOIUrl":"https://doi.org/10.1002/cbf.70097","url":null,"abstract":"<div>\u0000 \u0000 <p>Metabolic reprogramming plays an essential role in the initiation and aggressiveness of malignant tumors. This study aims to establish a prognostic signature for Wilms tumor (WT) based on metabolism-related genes (MRGs) and to explore potential molecular mechanisms. RNA sequencing data of WT samples and MRGs were sourced from GEO, TCGA and KEGG, respectively. Prognosis-associated differentially expressed MRGs (DE-MRGs) and Lasso regression were employed to create a nine-gene prognostic signature. Internal validation was conducted through bootstrap resampling. Prognostic performance was assessed through Kaplan–Meier curves, ROC curves, the C-index, and calibration curves. Additional analyses encompassed signaling pathways and chemotherapy response prediction. The expression levels and biological functions of <i>NNMT</i> were experimentally validated. A nine-gene signature comprising <i>B3GAT2, COLGALT2, CYP27C1, GAD2, GSTM5, LPIN3, NNMT, ST6GALNAC1</i> and <i>TCIRG1</i> was established. The risk score derived from this signature was shown to be an independent prognostic predictor and significantly associated with immune function and autophagy. <i>NNMT</i> expression levels were validated in both cells and tissues. Further experiments in vivo and in vitro indicated that <i>NNMT</i> might influence cholesterol efflux via <i>PPARG-LXRα</i> pathway, thereby enhancing cell proliferation and migration. This study established a metabolism-related gene signature to predict the prognosis of patients with WT. The findings may provide a promising tool for personalized diagnosis and treatment.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 7","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0