Cell Biochemistry and Function最新文献

{"title":"Evaluating the Effectiveness of a Novel Pongamia pinnata Derived Herbal Mouth-Dissolving Film for Treating Oral Disorders and Evaluating Its Anticancer Properties.","authors":"Devang Prajapati, Masuma Hakim, Margi Patel, Mohammad Javed Ansari, Saleh Alfarraj, Sanjay Chauhan, Vaibhav Bhatt, Virendra Kumar Yadav, Dipak Kumar Sahoo, Kashyap Thummar, Ashish Patel","doi":"10.1002/cbf.70049","DOIUrl":"https://doi.org/10.1002/cbf.70049","url":null,"abstract":"<p><p>The present study aimed to optimize a mouth-dissolving film (MDF) made from Pongamia pinnata stem bark extract to increase patient compliance and accelerate oral disease therapy. Several stem bark extracts were prepared, and karanjin was used as an herbal marker for the extracts. The ethanolic extract showed the maximum yield (12.10% ± 0.09%) and cytotoxic activity against human oral cancer (KB 3-1) and embryonic kidney cell lines. The MDF formulation was focused on incorporating a fixed amount of the extract and varying concentrations of HPMC E5 polymer, along with evaluating the performance of plasticizers like PEG 400 and propylene glycol (PG). An optimized formulation was determined based on disintegration time, wetting time, and folding endurance. The formulation consisted of HPMC E5 as a film-forming polymer, PG as a superior plasticizer, ascorbic acid as an antioxidant, and other ingredients contributing to solubility, dispersion, sweetening, and appearance. High-performance thin-layer chromatography-mass spectrometry analysis confirmed higher levels of karanjin in the optimized formulation, ensuring its successful incorporation and stability. Taste masking evaluations indicate a favorable taste profile and a high potential for patient compliance. The stability study displayed no significant changes in the physical characteristics of the film, affirming its stability and quality. In conclusion, the developed herbal-based optimized MDF presents a promising drug delivery system, offering enhanced patient compliance, taste masking, and stability. The MDF holds great potential for effective treatment and management of oral diseases, providing convenience and improved therapeutic outcomes.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 2","pages":"e70049"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility of Novel Marine Collagen on Periodontal Ligament Fibroblasts: A Pathway to Enhanced Regenerative Therapies.","authors":"Yasir Dilshad Siddiqui, Erry Mochamad Arief, Muhammad Aliyan Saddique","doi":"10.1002/cbf.70040","DOIUrl":"https://doi.org/10.1002/cbf.70040","url":null,"abstract":"<p><p>Body wall of sea cucumber Bohadschia bivittate contains a protein consisting of highly insoluble collagen fibers. We aimed to evaluate the biocompatibility and cytotoxicity of nonirradiated or γ-irradiated pepsin soluble collagen (PSC) extracted from Bohadschia bivittata on human periodontal ligament fibroblasts cells. The MTT assay showed significant increase in the cell viability values indicating that PSC is noncytotoxic. Further, nonirradiated PSC showed higher cell viabilities values than γ-irradiated PSC at all concentration, especially at an optimal dilution of 25% (p < 0.05). This exploratory study suggests that PSC from Bohadschia bivittata could be utilized as a novel biomaterial in periodontal regenerative therapies.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70040"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shh Protects the Injured Spinal Cord in Mice by Promoting the Proliferation and Inhibiting the Apoptosis of Nerve Cells via the Gli1-TGF-β1/ERK Axis.","authors":"Yunfei Liu, Meihua Hou, Jingran Wang, Xiaoying Li, Bichao Zhang, Juntang Lin, Ciqing Yang","doi":"10.1002/cbf.70038","DOIUrl":"10.1002/cbf.70038","url":null,"abstract":"<p><p>Spinal cord injury (SCI) is a common neurological trauma that cannot be completely cured with surgical techniques and medications. In this study, we established a mouse SCI model and used an adeno-associated virus (AAV) to achieve the high expression of sonic hedgehog (Shh) at the injury site to further investigate the therapeutic effect and mechanism of Shh on SCI. The results of the present study show that Shh may promote motor function recovery. The present findings demonstrate the protective effect of Shh overexpression in SCI by regulating the proliferation and apoptosis of nerve cells at the site of SCI. Shh promotes the proliferation of early microglia, inhibits the proliferation of early astrocytes, and promotes the formation of neurons at the site of injury. In addition, Shh may inhibit apoptosis at the SCI site. The mechanism by which Shh regulates nerve cells at the site of SCI may involve glioma-associated oncogene 1 (Gli1). The present research indicates that Gli1 regulates the transforming growth factor-β (TGF-β) signaling pathway, inhibiting the classic TGF-β1/Smad signaling pathway and activating the TGF-β1/extracellular regulated protein kinase (ERK) signaling pathway. Collectively, these findings suggest that Shh is a regulatory molecule involved in nerve cell proliferation and apoptosis. High Shh expression can accelerate motor function recovery after SCI, indicating that it may be a promising therapeutic approach for SCI.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70038"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Gene Editing in Triple-Negative Breast Cancer Research.","authors":"Shuying Feng, Jixia Li, Aifen Yan, Xiangxing Zhu, Ligang Zhang, Dongsheng Tang, Lian Liu","doi":"10.1002/cbf.70044","DOIUrl":"https://doi.org/10.1002/cbf.70044","url":null,"abstract":"<p><p>With the rapid development of gene editing technology, its application in breast cancer has gradually become the focus of research. This article reviews the application of gene editing technology in the treatment of breast cancer, and discusses its challenges and future development directions. The key application areas of gene editing technology in the treatment of breast cancer will be outlined, including the discovery of new therapeutic targets and the development of drugs related to the pathway. Gene editing technology has played an important role in the discovery of new therapeutic targets. Through the use of gene editing technology, breast cancer-related genes are systematically edited to regulate key regulatory factors on related pathways or key tumor suppressor genes such as FOXC1 and BRCA, and the results are analyzed in cell or animal experiments, and the target is obtained from the experimental results, which provides important clues for the development of new drugs. This approach provides an innovative way to find more effective treatment strategies and inhibit tumor growth. In addition, gene editing technology has also promoted the personalization of breast cancer treatment. By analyzing a patient's genomic information, researchers can pinpoint key genetic mutations in a patient's tumor and design personalized treatments. This personalized treatment approach is expected to improve the therapeutic effect and reduce adverse reactions. Finally, the application of gene editing technology also provides support for the development of breast cancer immunotherapy. By editing immune cells to make them more potent against tumors, researchers are trying to develop more effective immunotherapies to bring new treatment options to breast cancer patients.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70044"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in the Study of TAp73 and Sperm Apoptosis.","authors":"Ziao Liu, Min Pan, Jingya Li, Li Li, Tongsheng Wang","doi":"10.1002/cbf.70042","DOIUrl":"https://doi.org/10.1002/cbf.70042","url":null,"abstract":"<p><p>The study of the mechanism of oligoasthenospermia, which is a major cause of male infertility, has been the focus of research in the field of male reproduction. TAp73, a member of the p53 family of oncogenes, is endowed with tumor-suppressing activity due to its structural and functional homology with p53. It has been found that TAp73, plays a key role in spermatogenesis and maintaining male reproduction. When TAp73 is low-expressed or absent, the process of spermatogenesis is severely impaired, and mice deficient in TAp73 exhibit spermatogonial DNA damage, disturbed apical cytoplasmic specialization, and spermatocyte malformations resulting in reduced male fertility. Nevertheless, when TAp73 is overexpressed, it not only drives exogenous death receptors to regulate germ cell apoptosis, but also interacts with its various substrate proteins to promote the translocation of cytoplasmic Bax proteins to the mitochondria, resulting in the upregulation of the Bax/Bcl-2 ratio on the mitochondrial membrane and triggering a series of mitochondrial apoptotic effects. In this article, we will analyze the mechanism of TAp73 and sperm apoptosis, and elaborate the mechanism of TAp73 upregulation, exogenous apoptosis pathway and mitochondrial apoptosis pathway to systematically explain that the process of apoptosis induced by high expression of TAp73 is not fixed and single, but is interconnected, so as to provide a basis for the treatment of oligoasthenospermia and the research and development of new drugs using TAp73 as a target.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70042"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"SIX1 Is Upregulated in Gastric Cancer and Regulates Proliferation and Invasion by Targeting the ERK Pathway and Promoting Epithelial-Mesenchymal Transition\".","authors":"","doi":"10.1002/cbf.70045","DOIUrl":"https://doi.org/10.1002/cbf.70045","url":null,"abstract":"","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70045"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Synthesis and Characterization of Silver Nanoparticles With Cyanobacterium Oscillatoria salina Using Against MDR Pathogenic Bacteria and Their Antiproliferative and Toxicity Study.","authors":"Ajit Kumar Bishoyi, Chinmayee Priyadarsani Mandhata, Chita Ranjan Sahoo, Priyanka Samal, Debasmita Dubey, Bigyan Ranjan Jali, Abdulaziz Mohammed Alamri, Mohd Shahnawaz Khan, Rabindra Nath Padhy","doi":"10.1002/cbf.70043","DOIUrl":"https://doi.org/10.1002/cbf.70043","url":null,"abstract":"<p><p>The biosynthesis of silver nanoparticles (AgNPs) using cyanobacteria has gained significant attention due to its cost-effective and eco-friendly advantages in green synthesis. Additionally, biogenic AgNPs show great potential for biological applications, particularly in combating infections caused by drug-resistant bacteria and fungi. This study synthesized using the cyanobacterium Oscillatoria salina (Os-AgNPs). The Os-AgNPs were characterized by a UV-vis spectral absorption peak at 447 nm, and their functional groups were identified through X-ray diffraction analysis, revealing a crystal structure with a 2θ value of 38°. Transmission electron microscopy (TEM) analysis showed an average nanoparticle size of 9.81 nm. The Os-AgNPs demonstrated remarkable antioxidant, antibacterial, and antifungal properties. Their antibacterial activity was tested against multidrug-resistant (MDR) Gram-positive bacteria, including Staphylococcus aureus, Streptococcus pyogenes, and Enterococcus faecalis, as well as Gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, all isolated from clinical samples. The inhibition zones for bacterial strains ranged from 15 to 20 mm, as measured by the agar-well diffusion method. Similarly, the Os-AgNPs exhibited antifungal activity, with 20-30 mm inhibition zones against pathogenic fungi Trichophyton rubrum and Candida tropicalis. Additionally, the antiproliferative effects of the Os-AgNPs were evaluated on human cancer cell lines, including HeLa (cervical adenocarcinoma) and MD-AMB-231 (breast adenocarcinoma). In vivo toxicity studies were conducted using Swiss mouse models to assess the cytotoxic effects. Overall, the results suggest that Os-AgNPs, biosynthesized using O. salina, hold promise as potential antimicrobial and anticancer agents for pharmaceutical applications.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70043"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D and Endometriosis: Is There a Mechanistic Link?","authors":"Bethany Scout Jennings, Martin Hewison","doi":"10.1002/cbf.70037","DOIUrl":"10.1002/cbf.70037","url":null,"abstract":"<p><p>Endometriosis is a prevalent chronic gynaecological disorder, but its cause is still unclear, and both genetic and environmental factors may contribute disease aetiology. Prominent amongst the latter is vitamin D which can be obtained either by the action of sunlight on skin or from dietary sources. Serum levels of the main circulating form of vitamin D, 25-hydroxvitamin D (25(OH)D), have been reported to be inversely correlated with endometriosis, suggesting that vitamin D-deficiency may be a risk factor for the disease. Crucially, the active form of vitamin D, 1,25-dihydroxyvitamin D (1,25(OH)₂D) is known to exert many functions beyond its established role in the endocrinology of mineral homoeostasis and prevention of rickets. Several of these extra-skeletal effects of 1,25(OH)₂D may impact the risk and progression of endometriosis. The following review details the studies that have assessed associations between vitamin D status/supplementation and endometriosis severity and disease progression, but also describes the mechanistic targets for 1,25(OH)₂D in endometriosis with specific reference to immunomodulatory responses and effects on angiogenesis. Endometriosis is an under-reported health issue with poor non-invasive options for diagnosis. Given that vitamin D-deficiency may trigger or exacerbate key pathophysiological responses linked to endometriosis, analysis of vitamin D status in women may provide an alternative risk marker for endometriosis. Treatment options for endometriosis are also limited and the review will also consider whether vitamin D supplementation has a role in the management of endometriosis, either in prevention or treatment.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70037"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11686426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mettl14 and Mettl3 Work Cooperatively to Regulate Retinal Development.","authors":"Dan Chen, Yanling Xin, Jingyi Guo, Shuyi Chen","doi":"10.1002/cbf.70039","DOIUrl":"https://doi.org/10.1002/cbf.70039","url":null,"abstract":"<p><p>N⁶-methylenadenosine (m⁶A) modification, the most abundant epitranscriptomic modification in eukaryotic mRNAs, has been shown to play crucial roles in regulating various aspects of mRNA metabolism and functions. In this study, we applied the Cre-Loxp conditional knockout system to investigate the role of the core components of the m⁶A methyltransferase complex, METTL14 and METTL3, in retinal development. Our results showed that the double absence of Mettl14 and Mettl3 caused structural disturbance in the retina and prolonged the proliferation activity of retinal progenitor cells. Interestingly, the deletion of Mettl14 and Mettl3 did not affect the generation of various retinal cells, but severely disrupted their distribution. In addition, double deletion of Mettl14 together with Mettl3 caused a stronger phenotype than did single deletion of Mettl14. In conclusion, our study demonstrated that Mettl14 and Mettl3 work cooperatively to regulate retinal development.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70039"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Gold Nanoparticles-Conjugated Quercetin Induces Apoptosis via Inhibition of EGFR/PI3K/Akt-Mediated Pathway in Breast Cancer Cell Lines (MCF-7 and MDA-MB-231)''.","authors":"","doi":"10.1002/cbf.70041","DOIUrl":"https://doi.org/10.1002/cbf.70041","url":null,"abstract":"","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 1","pages":"e70041"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}