Cell Biochemistry and Function最新文献

Spacer-Complementary Single-Stranded DNA Oligonucleotides Can Serve as Target-Specific Inhibitors in CRISPR/Cas9 Systems 间隔互补单链DNA寡核苷酸可作为CRISPR/Cas9系统中的靶向特异性抑制剂

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-21 DOI: 10.1002/cbf.70088

Ruiying Han, Xiang Gao, Yiqi Qi, XiaoDan Lu, Xiaoli Wang, Xiaochuan Tang

{"title":"Spacer-Complementary Single-Stranded DNA Oligonucleotides Can Serve as Target-Specific Inhibitors in CRISPR/Cas9 Systems","authors":"Ruiying Han, Xiang Gao, Yiqi Qi, XiaoDan Lu, Xiaoli Wang, Xiaochuan Tang","doi":"10.1002/cbf.70088","DOIUrl":"https://doi.org/10.1002/cbf.70088","url":null,"abstract":"<div>\u0000 \u0000 <p>The continuous expression of the CRISPR/Cas system in organisms can lead to various potential issues. Some anti-CRISPR strategies have been developed to achieve precise control over CRISPR/Cas, yet these strategies are predominantly protein-based, with the most commonly used anti-CRISPR (Acr) proteins lacking sufficient target specificity. However, in this study, we designed a single-stranded DNA (ssDNA) inhibitor that was complementary to the spacer region on the guide RNA, operating at the nucleic acid level. We demonstrated that this method effectively inhibits the cleavage activity of Cas9-sgRNA ribonucleoprotein (RNP) in a target-specific manner through in vitro cleavage experiments. Furthermore, we explored the binding position and effective length of this inhibitory ssDNA, finding that its inhibitory effect was significantly reduced when the length of continuous complementarity with the 5′ end of the spacer was less than 7nt. The truncated ssDNA also showed potential in reducing off-target effects. Moreover, we applied nucleic acid inhibitors to embryos via microinjection, and gene editing activity was significantly reduced, as evidenced by a decrease in the mosaicism rate of mouse embryos undergoing normal gene editing from (84.4 ± 4.4) % to 0%. Our study introduces a convenient and target-specific nucleic acid inhibitor capable of achieving precise regulation of gene editing.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144331903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roscovitine Improved the Cytoplasmic Maturation of Bovine Oocytes and Subsequent Embryo Quality 罗斯科维汀促进牛卵母细胞细胞质成熟和随后的胚胎质量

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-19 DOI: 10.1002/cbf.70096

Feng Li, Junrui Zhang, Wanjie Wang, Xinyi Zhou, Huiying Zou, Haisheng Hao, Xueming Zhao, Yunhai Zhang, Weihua Du

{"title":"Roscovitine Improved the Cytoplasmic Maturation of Bovine Oocytes and Subsequent Embryo Quality","authors":"Feng Li, Junrui Zhang, Wanjie Wang, Xinyi Zhou, Huiying Zou, Haisheng Hao, Xueming Zhao, Yunhai Zhang, Weihua Du","doi":"10.1002/cbf.70096","DOIUrl":"https://doi.org/10.1002/cbf.70096","url":null,"abstract":"<div>\u0000 \u0000 <p>This study examined the effects of different concentrations of roscovitine (ROSC) on cytoplasmic maturation in bovine oocytes and subsequent embryo development. Oocytes were prematured with ROSC at concentrations of 0, 25, 37.5, 50 and 75 µM for 6 h. A significant increase in the number of oocytes at GV stage was observed in the treatment groups compared to the control group, indicating an inhibitory effect of ROSC on meiosis. After continued culture in a maturation solution without ROSC for 18 h, oocytes did not exhibit significant difference in maturation rates between the treatment and control groups, suggesting that the inhibitory effect of ROSC is reversible. Further analysis revealed that a 37.5 µM ROSC treatment significantly enhanced homogeneous mitochondrial distribution, mitochondrial membrane potential, and glutathione levels in mature oocytes, while reducing reactive oxygen radical content. IVF blastocysts from oocytes treated with 37.5 μM ROSC had a significant increase in the total cell number, and inner cell mass/total cell number ratio, and a reduction in apoptosis rate, despite no significant differences in cleavage or blastocyst rates compared to controls. These findings suggested that prematuration with 37.5 µM ROSC promoted cytoplasmic maturation in bovine oocytes and improved embryo quality. This study analyzed comprehensively the impacts of meiosis arrest induced by ROSC on cytoplasmic maturation in oocyte and embryo quality from various perspectives for the first time.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Comparison of Two Python-Based Programs for the Automated Analysis of Tight-Junction Phenotype in Brain Endothelium During Bacterial Infection 细菌感染期间脑内皮紧密连接表型自动分析两种python程序的体外比较

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-16 DOI: 10.1002/cbf.70093

Henry D. Mauser, Janessa Caroza, Shane Nicole Homez, Alyssa S. Arnett, William D. Cutts, Daryl W. Lam, Justin Thornton, Walter Adams, Brandon J. Kim

{"title":"In Vitro Comparison of Two Python-Based Programs for the Automated Analysis of Tight-Junction Phenotype in Brain Endothelium During Bacterial Infection","authors":"Henry D. Mauser, Janessa Caroza, Shane Nicole Homez, Alyssa S. Arnett, William D. Cutts, Daryl W. Lam, Justin Thornton, Walter Adams, Brandon J. Kim","doi":"10.1002/cbf.70093","DOIUrl":"https://doi.org/10.1002/cbf.70093","url":null,"abstract":"<p>Tight junction complexes are crucial features of brain endothelial cells, as they restrict the paracellular route across the blood–brain barrier. Tight junction disruption has been observed in conjunction with numerous diseases of the CNS. In such cases, the organization or integrity of cell–cell junctions may be analyzed with a variety of automated computer programs that quantitatively assess junction images. Here, we directly compare two previously developed python-based programs—JAnaP and IJOQ— for the semi- or fully automated analysis of tight junctions in human stem cell-derived brain-like endothelial cells. Cells were infected with <i>S. pneumoniae</i> and <i>S. agalactiae</i> to initiate junction disruption, and occludin and ZO-1 were analyzed in mock and infected groups via JAnaP and IJOQ. JAnaP and IJOQ both yielded comparable results for the quantification of tight junction disruption in brain endothelial cells. While JAnaP rendered data at the cellular level and gave more information regarding junction phenotype, IJOQ significantly reduced user time and eliminated potential user bias. Our results suggest that JAnaP and IJOQ are both appropriate for quantifying tight junction integrity in brain endothelial cells, and both may offer distinct advantages depending on their context of use.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbf.70093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Green Tea Ameliorate Obesity in Mice Kept at Thermoneutrality by Modulating Skeletal Muscle Metabolism? 绿茶是否通过调节骨骼肌代谢来改善热中性小鼠的肥胖？

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-16 DOI: 10.1002/cbf.70094

Celso Pereira Batista Sousa-Filho, Marcus Vinicius Aquino Silva, Victória Silva, Kauan Lima, Allanis Valon, Isabela Fiorentino Souza Nascimento, Maria Angélica Spadella, Rosemari Otton

{"title":"Does Green Tea Ameliorate Obesity in Mice Kept at Thermoneutrality by Modulating Skeletal Muscle Metabolism?","authors":"Celso Pereira Batista Sousa-Filho, Marcus Vinicius Aquino Silva, Victória Silva, Kauan Lima, Allanis Valon, Isabela Fiorentino Souza Nascimento, Maria Angélica Spadella, Rosemari Otton","doi":"10.1002/cbf.70094","DOIUrl":"https://doi.org/10.1002/cbf.70094","url":null,"abstract":"<p>The effects of green tea on metabolic diseases such as obesity and diabetes have been extensively studied. Obesity often leads to insulin resistance, particularly in peripheral tissues such as skeletal muscle. Green tea has shown promise in mitigating insulin resistance in several diet-induced obesity models. However, its ability to improve insulin sensitivity by modulating skeletal muscle metabolism in the absence of metabolic stress, such as constant cold exposure, remains unclear. Therefore, the aim of this study was to evaluate the effect of green tea on skeletal muscle metabolism in high-fat diet (HFD)-induced obese mice maintained at thermoneutrality (28°C). Male C57BL/6 mice were fed a control diet or an HFD for 4 weeks. Then, the HFD group mice were treated with green tea extract (500 mg/kg of body weight) while maintained at thermoneutrality (28°C). At the end of the experimental protocol, we performed metabolic analyses. This study suggested that green tea treatment attenuates the negative effects of HFD by improving muscle fiber cross-sectional area in the gastrocnemius muscle and increasing the expression of genes involved in lipid metabolism. Although no effect was observed on fatty acid oxidation, green tea improved insulin and glucose sensitivity, as evidenced by glucose and insulin tolerance tests. It also increased the expression of genes associated with glucose uptake and lactate dehydrogenase activity in skeletal muscle. These findings suggest that green tea treatment improves insulin sensitivity by influencing skeletal muscle metabolism even in obese mice maintained at thermoneutrality.</p>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cbf.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PINK1 Deficiency Facilitates Palmitic Acid-Induced Inflammation by Disrupting Mitochondrial Function to Activate mtDNA-cGAS-STING Signaling PINK1缺乏通过破坏线粒体功能激活mtDNA-cGAS-STING信号通路促进棕榈酸诱导的炎症

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-15 DOI: 10.1002/cbf.70092

Bin Ye, Yingting Pei, Henian Li, Yuqi Jiang, Wenying Jin, Yueqiu Gao, Wen Liu, Xin Guan, Yu Qiao, Xu Gao, Yanfen Zhang, Ning Ma, Hao Chang

{"title":"PINK1 Deficiency Facilitates Palmitic Acid-Induced Inflammation by Disrupting Mitochondrial Function to Activate mtDNA-cGAS-STING Signaling","authors":"Bin Ye, Yingting Pei, Henian Li, Yuqi Jiang, Wenying Jin, Yueqiu Gao, Wen Liu, Xin Guan, Yu Qiao, Xu Gao, Yanfen Zhang, Ning Ma, Hao Chang","doi":"10.1002/cbf.70092","DOIUrl":"https://doi.org/10.1002/cbf.70092","url":null,"abstract":"<div>\u0000 \u0000 <p>Metabolic cells exhibit low-grade chronic inflsammation characterized by excessive production and secretion of proinflammatory cytokines and chemokines in response to overnutrition and energy excess. Mitochondrial dysfunction is closely associated with metabolic inflammation. PINK1 (phosphatase and tensin homology-induced putative kinase 1) is a crucial pathway controlling mitochondrial autophagy, essential for maintaining mitochondrial quality control and metabolic homeostasis. The aim of this study was to investigate the role of PINK1 in metabolic inflammation. Our findings indicate that in adipocytes, palmitic acid (PA) activates the expression of PINK1. Additionally, knockdown of <i>PINK1</i> exacerbates PA-induced adipocyte inflammation. Mechanistically, <i>PINK1</i> deficiency impairs mitochondrial function, leading to the release of mtDNA and further activation of the cGAS-STING pathway. Therefore, targeting mitochondrial autophagy in adipocytes and the cGAS-STING pathway may represent effective approaches to alleviate the chronic inflammation associated with obesity and related metabolic disorders.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic and Therapeutic Insights Into the Function of Netrin1 in the Joint Tissue of Osteoarthritis Netrin1在骨关节炎关节组织中的作用机制及治疗意义

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-15 DOI: 10.1002/cbf.70095

Yuzhe Guan, Huifei Tian, Qian chen, Xiaoyan Chen, Zuping Wu

{"title":"Mechanistic and Therapeutic Insights Into the Function of Netrin1 in the Joint Tissue of Osteoarthritis","authors":"Yuzhe Guan, Huifei Tian, Qian chen, Xiaoyan Chen, Zuping Wu","doi":"10.1002/cbf.70095","DOIUrl":"https://doi.org/10.1002/cbf.70095","url":null,"abstract":"<div>\u0000 \u0000 <p>Osteoarthritis is the most prevalent degenerative joint disease, characterized by the trauma and inflammation of the cartilage and subchondral bone, leading to cartilage degradation, reduced bone mass, joint pain, and functional impairment. Articular cartilage and subchondral bone is a complex functional structural complex, in which Netrin protein family and its transmembrane protein receptors such as Unc5b, DCC and A2B are widely expressed. The Netrin family, part of the laminin superfamily, includes various subtypes, with Netrin1 being notably researched for its regulatory role in bone tissue.Netrin1 protein can activate downstream signaling pathways that affect cell function and regulate the functional activity of various cells in joint tissue after binding to its receptors. Studies have pointed out that anti-Netrin1 treatment can significantly alleviate osteoarthritis and other arthritis lesions, as well as reduce neuropathic pain. This review aims to explore the function of the Netrin1 protein and its receptors across joint structures, discussing their potential regulatory mechanisms and implications for treating diseases like osteoarthritis.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144292857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Bacillus Subtilis BN Strain on M1 Macrophage Antiviral Response 枯草芽孢杆菌BN株对M1巨噬细胞抗病毒应答的影响

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-14 DOI: 10.1002/cbf.70091

Keisuke Tobita, Manami Sawahata, Keiichiro Imaizumi, Keiko Kotsuna, Takanori Miyoshi

{"title":"Effect of Bacillus Subtilis BN Strain on M1 Macrophage Antiviral Response","authors":"Keisuke Tobita, Manami Sawahata, Keiichiro Imaizumi, Keiko Kotsuna, Takanori Miyoshi","doi":"10.1002/cbf.70091","DOIUrl":"https://doi.org/10.1002/cbf.70091","url":null,"abstract":"<div>\u0000 \u0000 <p>M1 macrophages play an important role in pathogenic microorganism infection. Natto, a traditional fermented food from Japan, has long been thought to prevent infection by pathogenic microorganisms<i>. Bacillus subtilis</i> BN strain, which has been isolated from natto, is used in animal feed, health food products, and pharmaceuticals as a probiotic capable of regulating intestinal flora and immunomodulates. The purpose of this study is to investigate the effects of <i>B. subtilis</i> BN strain on the antiviral immune function of human M1 macrophages. The M1 macrophages studied here were derived from a human monocytic cell line (THP-1). Overall, we found that <i>B. subtilis</i> BN strain enhanced gene expressions of antiviral and anti-inflammatory cytokines in M1 macrophages and M1 macrophages stimulated with resiquimod in a cell culture model that mimics single-stranded RNA virus infection. In M1 macrophages, <i>B. subtilis</i> BN strain was also found to promote the gene expression and phosphorylation of key molecules in the toll-like receptor signaling pathways. Furthermore, the BN strain induced the expression of some cytokine mRNA was reduced by the knockdown of NFκB in M1 macrophages. Taken together, these results suggest that toll-like receptor signal pathways are involved in the regulation of antiviral and anti-inflammatory cytokine gene expressions induced by the BN strain in M1 macrophages.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Fascin1 and Pak1 Expressions Enhance Age-Associated B-Cell Actin Cytoskeleton Remodeling and Motility 增加的Fascin1和Pak1表达增强与年龄相关的b细胞肌动蛋白细胞骨架重塑和运动

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-11 DOI: 10.1002/cbf.70090

Mitsuhiro Fujiwara, Ryohei Kondo, Yuma Sugiyama, Mitsuo Maruyama, Akihiko Nishikimi

{"title":"Increased Fascin1 and Pak1 Expressions Enhance Age-Associated B-Cell Actin Cytoskeleton Remodeling and Motility","authors":"Mitsuhiro Fujiwara, Ryohei Kondo, Yuma Sugiyama, Mitsuo Maruyama, Akihiko Nishikimi","doi":"10.1002/cbf.70090","DOIUrl":"https://doi.org/10.1002/cbf.70090","url":null,"abstract":"<div>\u0000 \u0000 <p>Age-associated B cells (ABCs), an atypical B-cell subset, tend to accumulate with age in mice and humans. These cells exhibit distinct characteristics, such as the ability to secrete antibodies and inflammatory cytokines upon stimulation of Toll-like receptor 7 (TLR7) and TLR9. Additionally, ABCs have been found to be more efficient in presenting antigens to T cells than follicular (FO) B cells. These features contribute to the development of pathogenic phenotypes in aging individuals. In this study, we demonstrated that actin cytoskeleton remodeling was enhanced in CD11b<sup>+</sup>/CD11c<sup>+</sup> ABCs compared to CD11b<sup>−</sup>/CD11c<sup>−</sup> B cells. ABCs exhibited higher motility across Transwell membranes and three-dimensional (3D) collagen gels, even without chemoattractants. Due to the remodeling of chemokine receptor expression, ABCs were attracted by CXCL12 and CCL21 rather than CXCL13. Among F-actin remodeling-related factors, expression levels of Fascin1 and Pak1 were increased in ABCs. Treatment with the Pak1 inhibitor, IPA3, significantly attenuated ABC migration in Transwell chambers and 3D collagen gels. In contrast, the Fascin1 inhibitor, migrastatin, only reduced ABC migration in the 3D collagen gel. The increased expression of Fascin1 and Pak1 enhances actin cytoskeleton remodeling in ABCs, facilitating their dispersion within secondary lymphoid tissues.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress of p53 Involvement in Central Nervous System Diseases and Targeted Drug Discovery p53参与中枢神经系统疾病及靶向药物的研究进展

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-10 DOI: 10.1002/cbf.70089

Fenglian Xu, Zijun Liu, Ziyu Wang, Yi Zhang, Yu Zhao, Weirong Fang

引用次数: 0

Animal Models: A Tool for Colon Cancer Research 动物模型：结肠癌研究的工具

IF 2.8 3区生物学

Cell Biochemistry and Function Pub Date : 2025-06-10 DOI: 10.1002/cbf.70087

LokeshKumar Boopathy, Sasikumar Arumugam, Kumar Ponnusamy, Siddarth Srigokul Kumar, Thiyagarajan Gopal, Saravanan Durai, Sriramkumar Ravikumar, Madan Kumar Arumugam

{"title":"Animal Models: A Tool for Colon Cancer Research","authors":"LokeshKumar Boopathy, Sasikumar Arumugam, Kumar Ponnusamy, Siddarth Srigokul Kumar, Thiyagarajan Gopal, Saravanan Durai, Sriramkumar Ravikumar, Madan Kumar Arumugam","doi":"10.1002/cbf.70087","DOIUrl":"https://doi.org/10.1002/cbf.70087","url":null,"abstract":"<div>\u0000 \u0000 <p>Preclinical animal research plays a crucial role in studying colorectal cancer (CRC). Mouse models allow for testing the efficacy of potential medications and understanding the mechanisms behind CRC development. Carcinogens like 1,2-Dimethylhydrazine (DMH) and Azoxymethane (AOM) are used to induce CRC in these models, enabling researchers to investigate various treatment approaches. The availability of diverse animal strains facilitates the study of CRC progression and metastasis. By examining literature on animal models, researchers can assess the accuracy of reproducing different stages of CRC, from crypts to polyps and tumors. Animal models are essential for studying the tumor microenvironment and its impact on CRC. These models replicate key features of human CRC, including angiogenesis and immune responses. Both immunocompetent and immunocompromised rodents are used to evaluate immunotherapeutic drugs. This review summarizes common murine models for CRC and their applications in translational research. Future trends in this field likely include further advancements in animal models for studying tumor biology.</p></div>","PeriodicalId":9669,"journal":{"name":"Cell Biochemistry and Function","volume":"43 6","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144256229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0