Caryologia最新文献_第9页

Development of Female Gametophyte in Gladiolus italicus Miller (Iridaceae) 意大利剑兰(鸢尾科)雌性配子体的发育

IF 2.1 4区生物学

Caryologia Pub Date : 2021-12-21 DOI: 10.36253/caryologia-1082

Çiler Kartal, Nuran Ekici, Almina Kargacıoğlu, Hazal Nurcan Ağırman

引用次数: 0

Molecular identification and genetic relationships among Alcea (Malvaceae) species by ISSR Markers: A high value medicinal plant 高价值药用植物锦葵属植物的ISSR分子鉴定及其亲缘关系

IF 2.1 4区生物学

Caryologia Pub Date : 2021-12-21 DOI: 10.36253/caryologia-1330

Jinxin Cheng, Dingyu Hu, Yaran Liu, Zetian Zhang, M. Khayatnezhad

{"title":"Molecular identification and genetic relationships among Alcea (Malvaceae) species by ISSR Markers: A high value medicinal plant","authors":"Jinxin Cheng, Dingyu Hu, Yaran Liu, Zetian Zhang, M. Khayatnezhad","doi":"10.36253/caryologia-1330","DOIUrl":"https://doi.org/10.36253/caryologia-1330","url":null,"abstract":"Alcea L. is one of the largest genera of Malvaceae family with nearly 70 species worldwide mainly distributed in SW Asia. According to the latest revision of the family, it is represented by 34 species in the Flora of Iran, among them, 15 species are endemic. It is tough to accurate germplasm/ plant recognition by using morphological characteristics because of its propagation, growing and using. We conducted a molecular data analysis on these plant species due to their importance. We examined 156 plants from 14 species in 16 regions that were selected randomly for this investigation. It has been 119 polymorphic bands (94.33%) were resulted from 128 bands of 10 primers in amplification of genomic DNA. ISSR primers have a great capacity to detect polymorphic loci among Alcea species, as evidenced by the high average PIC and MI values found. The genetic similarity of 14 species was calculated and ranged between 0.635 to 0.990. Inter-Simple sequence repeats (ISSR) markers research revealed that Alcea tarica Pakravan & Ghahreman and Alcea kopetdaghensis lljin had the least similarity, while Alcea semnanica Pakravan and Alcea mazandaranica Pakravan & Ghahreman had the most. The current study attempts to answer three questions: 1) can ISSR markers identify Alcea species? 2) what is the genetic structure of these taxa in Iran? and 3) what is the inter-relationship between these taxa? The current study discovered that ISSR markers can be used to identify species.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"52 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86216486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The interacting effects of genetic variation in Geranium subg. Geranium (Geraniaceae) using scot molecular markers 天竺葵亚群遗传变异的互作效应。天竺葵(geranaceae)的苏格兰分子标记

IF 2.1 4区生物学

Caryologia Pub Date : 2021-12-21 DOI: 10.36253/caryologia-1079

Bo-Yi Shi, M. Khayatnezhad, A. Shakoor

{"title":"The interacting effects of genetic variation in Geranium subg. Geranium (Geraniaceae) using scot molecular markers","authors":"Bo-Yi Shi, M. Khayatnezhad, A. Shakoor","doi":"10.36253/caryologia-1079","DOIUrl":"https://doi.org/10.36253/caryologia-1079","url":null,"abstract":"One of the most crucial aspects of biological diversity for conservation strategies is genetic diversity, particularly in rare and narrow endemic species. Our study is the first attempt to utilize SCoT markers to check the genetic diversity in Iran. We used 115 plant samples. Our objectives were 1) to check genetic diversity among Geranium species 2) Genetic structure of the Geranium 3) Do the Geranium species exchange genes? 4) To detect isolation by distance among the Geranium species. We used traditional morphological and molecular methods to assess genetic diversity and genetic structure in the Geranium species. A total of 129 amplified polymorphic bands were generated across 13 Geranium species. The size of the amplified fragments ranged from 150 to 3000 bp. G. stepporum showed the highest values for the effective number of alleles (Ne = 1.30) and Shannon information index (I =0.35). Significant ANOVA results (P <0.01) showed differences in quantitative morphological characters in plant species. G. sylvaticum showed high genetic diversity. Mantel test showed a significant correlation (r = 0.17, p=0.0002) between genetic distance and geographical distance, so isolation by distance (IBD) occurred among the Geranium species. According to the SCoT markers analysis, G. kotschyi and G. dissectum had the lowest similarity, and the species of G. sylvaticum and G. pratense had the highest similarity. The present study revealed that a combination of morphological and SCoT methods could distinguish the species of Geranium.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"93 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86276498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Colchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae) 秋水仙碱诱导的羊草雄性减数分裂异常和2n花粉的表现斯普拉格(伞形科)

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-28 DOI: 10.36253/caryologia-1113

H. Dwivedi

{"title":"Colchicine induced manifestation of abnormal male meiosis and 2n pollen in Trachyspermum ammi (L.) Sprague (Apiaceae)","authors":"H. Dwivedi","doi":"10.36253/caryologia-1113","DOIUrl":"https://doi.org/10.36253/caryologia-1113","url":null,"abstract":"Unreduced gametes are the key source for the natural polyploidization in plants, but process of its formation is very low in nature. Meiotic mutants are second source for the formation of 2n pollen. In this cytological investigation, the meiotic aberrations and its impact on post-meiotic products were analysed in autotetraploid Trachyspermum ammi (L.) Sprague (4n=36). The seedlings of T. ammi (L.) Sprague were treated with 3 different concentrations of colchicine (0.2, 0.4 and 0.5%, w/v) for 3 different durations. Six polyploid plants were induced which was confirmed on the basis of cytological analysis. Colchicine, an anti-microtubular drug induced different meiotic and post-meiotic abnormalities such as chromosomal bridges, lagging chromosomes, scattering, precocious, fragments, dyads, triads, and polyads. The formation of several abnormal sporads clearly signifies the meiotic restitution. The tendency of univalents to scattered in the cytoplasm at metaphase was identified as a peculiar aberration asynapsis. Pollen variability and fusion of pollen walls was reported and pollen fertility was calculated. The morphological analysis of the pollen allowed us to confirm the occurrence of 2n pollen.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"107 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82228024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of chronic alcoholic intoxication and lighting regime on karyometric and ploidometric parameters of hepatocytes of rats 慢性酒精中毒及光照对大鼠肝细胞核倍体参数的影响

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-28 DOI: 10.36253/caryologia-1122

D. Areshidze, Y. Kirillov, L. Makartseva, M. Kozlova, I. Chernov, E. Shtemplevskaya

{"title":"Influence of chronic alcoholic intoxication and lighting regime on karyometric and ploidometric parameters of hepatocytes of rats","authors":"D. Areshidze, Y. Kirillov, L. Makartseva, M. Kozlova, I. Chernov, E. Shtemplevskaya","doi":"10.36253/caryologia-1122","DOIUrl":"https://doi.org/10.36253/caryologia-1122","url":null,"abstract":"The features of the diurnal dynamics of the area of rat hepatocyte nuclei and their ploidy were studied under conditions of a standart (fixed) light regime and constant illumination, as well as under chronic exposure to alcohol in the mentioned light regimes. It has been shown that exposure to alcohol and exposure to constant illumination separately lead to a change in the amplitude-phase characteristics of the circadian rhythm of the nucleus area, while the combined effect of these factors leads to a complete destruction of the rhythm, which indicates a violation of adaptation processes. An increase in the average ploidy of hepatocyte nuclei in chronic alcohol intoxication is also shown, while in animals kept under constant illumination without drinking alcohol, the values of this parameter decrease, which indicates a successful course of the adaptation process. The conducted research indicates that the results of karyometric and ploidometric analysis characterize the degree of influence of alcohol intoxication and changes in the light regime on the liver of rats, reflecting the rate of efficiency of adaptation to these factors.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"20 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76702822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemistry analytical, fish micronuclei and onion chromosome damage for assessing treatment effect SBR in the Domžale–Kamnik central WWTP and quality of river Kamniška Bistrica 化学分析、鱼微核和洋葱染色体损伤评价SBR在Domžale-Kamnik中央污水处理厂和Kamniška Bistrica河水质中的处理效果

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-27 DOI: 10.36253/caryologia-1177

Peter Firbas

{"title":"Chemistry analytical, fish micronuclei and onion chromosome damage for assessing treatment effect SBR in the Domžale–Kamnik central WWTP and quality of river Kamniška Bistrica","authors":"Peter Firbas","doi":"10.36253/caryologia-1177","DOIUrl":"https://doi.org/10.36253/caryologia-1177","url":null,"abstract":"Abstract The aim of this study was to evaluate the cytotoxic and genotoxic potential of the wastewater (WW), the effectiveness of the treatment used by the wastewater treatment plant (WWTP) with sequential batch reactors (SBR) technology, and whether its final treated effluent (FTE) can compromise the water quality of the river at the location where it is discharged. We focused our research on six examples. For analytical chemistry and Allium metaphase (M) test all six samples were collected. Of these, three are so-called biotechnological patterns (WW, WW after mechanical step treatment and FTE), and three are natural riverine environmental patterns. For the micronucleus (MN) test, fish specimens were collected from three sites in the river Kamniška Bistrica. The first two sites locations are up and down the FTE outlet. Results from these areas were compared to the third site (not polluted) reference site, the so-called natural control group. Complementary study with analytical chemistry and biological tests shows that the treatment effect SBR in the Domžale–Kamnik central WWTP carried effectively proved to be efficient for the removal of the cytogenotoxic substances in treated effluent and consequently in aquatic environment.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"66 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83844045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphological, Karyological and Phylogenetic Analyses of three Artemisia species that Around the Van Lake in Turkey 土耳其凡湖附近三种青蒿的形态、核学和系统发育分析

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-26 DOI: 10.36253/caryologia-1139

Pelin Yilmaz Sancar, S. Civelek, M. Kurşat

{"title":"Morphological, Karyological and Phylogenetic Analyses of three Artemisia species that Around the Van Lake in Turkey","authors":"Pelin Yilmaz Sancar, S. Civelek, M. Kurşat","doi":"10.36253/caryologia-1139","DOIUrl":"https://doi.org/10.36253/caryologia-1139","url":null,"abstract":"Artemisia is one of the biggest genera in the family Asteraceae, with around 500-600 taxa at specific and sub-specific levels and organised in five subgenera. Due to the high number of taxa, a lot taxonomists are trying to solve the problem of its classification and phylogeny but its natural classification still has not been achieved.The aim of this study is to try to solve the problematic systematic relationship between three different Artemisia species growing in close proximity to each other in the light of morphological, karyological and molecular data.The roots, stems, leaves, flowers structures of the plant samples collected from different populations belong to these species were investigated within the framework of morphological studies. Additionally, the chromosome counts and karyotype analysises of these species were made and idiograms were drawn in the karyological studies. In the context of phylogenetic studies, ITS and trn regions of 22 individuals belonging to 3 taxa were studied. It has been found that there is complete speciation genetic isolation mechanism between the species A. spicigera, A. taurica and A. fragrans that inhibit gene flow. A. fragrans and A. spicigera species are very similar to each other in terms of morphological characteristics. However, since populations of the species a fragrans are otopolyploid, the dimensional values of their morphological squares are larger than those of the species A. spicigera.This study is important as it is the first molecular based study relating with some species of Artemisia growing naturally in Turkey.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"67 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83164429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Further insights on chromosomal evolution of the genus Enyalius with karyotype description of Enyalius boulengeri Etheridge, 1969 (Squamata, Leiosauridae) 利用1969年布伦格里耶氏鳗属核型描述对鳗属染色体进化的进一步认识(鳞目，鳗科)

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-26 DOI: 10.36253/caryologia-1120

Cynthia Aparecida Valiati Barreto, M. A. Peixoto, Késsia Leite de Souza, N. Travenzoli, Renato Neves Feio, Jorge Abdala Dergam

{"title":"Further insights on chromosomal evolution of the genus Enyalius with karyotype description of Enyalius boulengeri Etheridge, 1969 (Squamata, Leiosauridae)","authors":"Cynthia Aparecida Valiati Barreto, M. A. Peixoto, Késsia Leite de Souza, N. Travenzoli, Renato Neves Feio, Jorge Abdala Dergam","doi":"10.36253/caryologia-1120","DOIUrl":"https://doi.org/10.36253/caryologia-1120","url":null,"abstract":"The genus Enyalius is composed of 10 described species inhabiting forest areas in Amozônia, Cerrado and Atlantic forest biomes. Currently, eight species with high levels of chromosome variation have been karyotyped. The study aims to characterize the karyotype of Enyalius boulengeri, with classical and molecular techniques, and improve knowledge about the karyotype evolution of the lizard genus Enyalius. The species has 2n = 36 chromosomes (8m + 4sm + 24mc), FN = 24; NORs and 18S rDNA were subtelomeric and located on chromosome pair 2. Repetitive DNA probes (CAT)10 accumulated on centromeric and terminal regions of some macrochromosomes. (GA)15 probe showed conspicuous accumulation on the pericentromeric region of chromosome pairs 1 and 6. Repetitive FISH patterns obtained with (GC)15 probe marked the pericentromeric region of the first chromosome pair. All probes showed accumulation in the microchromosomes. The chromosomal formula found on E. boulengeri has been considered the ancestral karyotype for pleurodont Iguania. The genus Enyalius is characterized by two distinctive chromosomal groups; one with highly conserved karyotypes, whereas the other is karyotypically diverse. Our molecular cytogenetics data are promising and will increase knowledge about the genus Enyalius chromosome evolution.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"347 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84857822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenetic and cytological analysis of advanced genetic material of cape gooseberry Physalis peruviana for use in plant breeding 猕猴桃高级遗传物质在植物育种中的细胞遗传学和细胞学分析

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-26 DOI: 10.36253/caryologia-1081

Clara Viviana FRANCO FLOREZ, Sara Alejandra LIBERATO GUIO, Erika Patricia SANCHEZ BETANCOURT, Francy Liliana GARCÍA ARIAS, V. M. Núñez Zarantes

{"title":"Cytogenetic and cytological analysis of advanced genetic material of cape gooseberry Physalis peruviana for use in plant breeding","authors":"Clara Viviana FRANCO FLOREZ, Sara Alejandra LIBERATO GUIO, Erika Patricia SANCHEZ BETANCOURT, Francy Liliana GARCÍA ARIAS, V. M. Núñez Zarantes","doi":"10.36253/caryologia-1081","DOIUrl":"https://doi.org/10.36253/caryologia-1081","url":null,"abstract":"The cape gooseberry, Physalis peruviana L., is a crop that is transitioning from a semi-wild rural food source to becoming an international export commodity fruit deserving of greater attention from the scientific community, producers, policy makers and opinion makers. Despite its importance, the crop has serious technological development challenges, mainly associated with the limited supply of genetically improved materials for producers and consumers. In the present study, the level of ploidy of 100 genotypes of gooseberry from a working collection was determined by counting the number of chromosomes and chloroplasts, to include them in the breeding program. The number of chromosomes in dividing cells of root-tip meristems, as well as the number of chloroplasts per guard cell, from plants grown in vitro and ex vitro conditions were determined. Haploid with 24 chromosomes, doubled haploid-tetraploid with 48 chromosomes, aneuploid (44 and 49 chromosomes) and mixoploid genotypes with 36 to 86 chromosomes were found. The number of chloroplasts / cell guard ranged from 4-8, 6-16, 7-16 and 9-21 for the haploid, aneuploid, doubled haploid-tetraploid and mixoploid genotypes, respectively. Evidence of a high cytogenetic diversity in the evaluated genotypes.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"1 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80735117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical evaluation of chromosomes of some Lathyrus L. taxa growing in Turkey 土耳其一些土藓属分类群染色体的统计评价

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-26 DOI: 10.36253/caryologia-1124

B. Yildirim, H. Genc, M. Açar, T. Çetin

引用次数: 1