Caryologia最新文献_第8页

Morphometric analysis and genetic diversity in Rindera (Boraginaceae-Cynoglosseae) using sequence related amplified polymorphism 利用序列相关扩增多态性分析牛虻(Boraginaceae-Cynoglosseae)的形态计量学和遗传多样性

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-1380

X. Yao, Haodong Liu, M. S. Tabarestani

{"title":"Morphometric analysis and genetic diversity in Rindera (Boraginaceae-Cynoglosseae) using sequence related amplified polymorphism","authors":"X. Yao, Haodong Liu, M. S. Tabarestani","doi":"10.36253/caryologia-1380","DOIUrl":"https://doi.org/10.36253/caryologia-1380","url":null,"abstract":"The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"22 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83460194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some molecular cytogenetic markers and classical chromosomal features of Spilopelia chinensis (Scopoli, 1786) and Tachybaptus ruficollis (Pallas, 1764) in Thailand 泰国中国Spilopelia chinensis (Scopoli, 1786)和ruficollis Tachybaptus (Pallas, 1764)的一些分子细胞遗传学标记和经典染色体特征

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-952

I. Patawang, Sarawut Kaewsri, S. Jantarat, P. Supanuam, Sarun Jumrusthanasan, A. Tanomtong

{"title":"Some molecular cytogenetic markers and classical chromosomal features of Spilopelia chinensis (Scopoli, 1786) and Tachybaptus ruficollis (Pallas, 1764) in Thailand","authors":"I. Patawang, Sarawut Kaewsri, S. Jantarat, P. Supanuam, Sarun Jumrusthanasan, A. Tanomtong","doi":"10.36253/caryologia-952","DOIUrl":"https://doi.org/10.36253/caryologia-952","url":null,"abstract":"This study analyzed the karyological features of two bird species – Spilopelia chinensis and Tachybaptus ruficollis – from Northeastern Thailand. Mitotic chromosomes were indirectly prepared by fibroblast cell culture. The chromosomes were stained by conventional Giemsa staining and microsatellite repeat of fluorescence in situ hybridization techniques. Giemsa staining showed that the diploid chromosome number of S. chinensis was 2n=70 and T. ruficollis was 60. The types of chromosomes observed in S. chinensis were 4 large metacentric, 2 medium acrocentric, 2 small metacentric, 2 small submetacentric, 2 sex chromosomes and 58 microchromosomes; the karyotype of T. ruficollis comprised 2 large metacentric, 2 large submetacentric, 2 large acrocentric, 8 small metacentric, 4 small submetacentric, ZW sex chromosomes and 40 microchromosomes. The molecular cytogenetical features that were exhibited only on the male T. ruficollis chromosome included two microsatellites and telomeric sequences: two signals of d(CA)15 on two microchromosomes, one signal of d(GC)15 on one of the first pair, and signals of AGGGTTn sequences on each telomeric region of all macro- and microchromosomes. The karyotype formula was deduced as: 2n (70) = Lm4 + Ma2 + Sm2 + Ssm2 + 2 sex chromosomes (Sm1/Ssm1) + 58 microchromosomes for S. chinensis and 2n (60) = Lm2 +Lsm2 + La2 + Sm8 + Ssm4 + Z (Msm1) W (Ssm1) + 40 microchromosomes for T. ruficollis.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"74 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87269487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Centromeric enrichment of LINE-1 retrotransposon in two species of South American monkeys Alouatta belzebul and Ateles nancymaae (Platyrrhini, Primates) 南美猕猴Alouatta belzebul和Ateles nancymaae (Platyrrhini，灵长类)两种猴LINE-1反转录转座子的着丝粒富集

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-1296

Simona Ceraulo, Milioto Vanessa, F. Dumas

引用次数: 4

Variation of microsporogenesis in sexual, apomictic and recombinant plants of Poa pratensis L. 草地早熟禾有性、无融合和重组植株小孢子发生的变异。

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-1375

E. Falistocco, G. Marconi, L. Raggi, D. Rosellini, M. Ceccarelli, E. Albertini

{"title":"Variation of microsporogenesis in sexual, apomictic and recombinant plants of Poa pratensis L.","authors":"E. Falistocco, G. Marconi, L. Raggi, D. Rosellini, M. Ceccarelli, E. Albertini","doi":"10.36253/caryologia-1375","DOIUrl":"https://doi.org/10.36253/caryologia-1375","url":null,"abstract":"Apomixis is a rather widespread phenomenon in plants. It is defined as the asexual formation of a seed from the maternal tissues of the ovule, avoiding the processes of meiosis and fertilization. Some species are facultative apomicts and form seeds by means of sexual and apomictic pathways to different extents. This is the case of Poa pratensis, the Kentucky bluegrass, which reproduces by aposporous pseudogamous facultative apomixis. This grass is one of the most studied apomictic systems, however some aspects, such as the male meiotic behavior, have not been so far investigated. In this study the process of microsporogenesis in genotypes of P. pratensis with a different mode of reproduction was investigated. The analysis revealed an almost regular meiosis in the sexual plants whereas apomictic genotypes exhibited different levels of meiotic irregularities, mainly due to cell fusion and irregular segregation in I and II division. Our data did not reveal evident connections between the extent and types of abnormalities and the components of apomixis, apomeiosis and parthenogenesis. The meiotic behavior of the examined plants was discussed in the light of their origin.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"10 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90200342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A meta-analysis of genetic divergence versus phenotypic plasticity in walnut cultivars (Juglans regia L.) 核桃品种遗传分化与表型可塑性的meta分析

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-1129

Melika Tabasi, M. Sheidai, F. Koohdar, Darab Hassani

{"title":"A meta-analysis of genetic divergence versus phenotypic plasticity in walnut cultivars (Juglans regia L.)","authors":"Melika Tabasi, M. Sheidai, F. Koohdar, Darab Hassani","doi":"10.36253/caryologia-1129","DOIUrl":"https://doi.org/10.36253/caryologia-1129","url":null,"abstract":"Persian walnut (Juglans regia L.), is very auspicious plant species of Iran from both economical and food points of views. Both wildly grown as well as cultivated forms of this plant species are scattered in different geographical regions of the country and are a valuable source for edible nut as well as job employment. Scattered published data on genetic diversity of this important plant species are mainly based on different molecular data analyses; therefore a meta-analysis of the same cultivars based on several different molecular markers including DNA-sequences and multi-locus markers was conducted to provide a detailed insight on genetic structure of walnuts. The results indicated a moderated genetic variability of about 40 percent in the studied cultivars; however these cultivars are genetically differentiated as revealed by Fst and AMOVA. HGT analyses revealed that the cultivars phylogeny differs to some degree by different markers and therefore a heat map was constructed to reveal the cultivars relationships based on combined molecular data. A higher Pst value was obtained compared to that of Fst genetic differentiation, therefore, it seems that local adaptation and selection have played role in the walnut cultivars’ morphological divergence. LFMM analysis identified some adaptive multi-locus alleles in the studied walnut cultivars.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"57 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89594605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The new chromosomal data and karyotypic variations in genus Salvia L. (Lamiaceae): dysploidy, polyploidy and symmetrical karyotypes 鼠尾草属新染色体资料及核型变异:异倍体、多倍体和对称核型

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-641

H. Eroğlu, E. Martin, A. Kahraman, Elif Gezer Aslan

{"title":"The new chromosomal data and karyotypic variations in genus Salvia L. (Lamiaceae): dysploidy, polyploidy and symmetrical karyotypes","authors":"H. Eroğlu, E. Martin, A. Kahraman, Elif Gezer Aslan","doi":"10.36253/caryologia-641","DOIUrl":"https://doi.org/10.36253/caryologia-641","url":null,"abstract":"In this study, it was aimed to determine the chromosome number of 21 Salvia L. species, to determine chromosome morphology, to reveal karyotype analysis in detail and to contribute to the cytotaxonomy of Salvia. In this context, the results are as follows: (i) the first report for the number of chromosomes of ten species, namely S. corrugata Vahl. (2n = 16), S. curviflora Benth. (2n = 16), S. darcyi J.Compton, S. greggii A.Gray, S. longifolia Nutt., S. vitifolia Benth. (2n = 22), S. subrotunda A.St.-Hil. ex Benth. (2n = 44), S. oppositiflora Ruiz & Pav. (2n = 56), S. stolonifera Benth. and S. atrocyanea Epling (2n = 60); (ii) the karyotypic variations and new chromosome numbers different from previous reports for three species, namely S. cardiophylla Benth. (2n = 36), S. cuspidata Ruiz & Pav. (2n = 44) and S. microphylla Sessé & Moc. (2n = 46); (iii) the same chromosome numbers from previous reports for eight species, namely S. campanulata Wall. ex Benth. (2n = 16), S. elegans Vahl. (2n = 20), S. involucrata Cav., S. mexicana Sessé & Moc. (2n = 22), S. apiana Jeps., S. leucophylla Greene, S. mellifera Greene (2n = 30), and S. splendens Ker Gawl. (2n = 44); (iv) the detailed chromosome measurements and karyotype analyses for all species studied for the first time; (v) the symmetrical karyotypes for all studied species; (vi) the variations resulting from dysploidy or polyploidy and discussing their reasons.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"41 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76320871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genetic variations and interspesific relationships in Lonicera L. (Caprifoliaceae), using SCoT molecular markers 利用SCoT分子标记分析忍冬属植物的遗传变异及种间关系

IF 2.1 4区生物学

Caryologia Pub Date : 2022-03-08 DOI: 10.36253/caryologia-1320

Fengzhen Chen, Dongmei Li, M. Farshadfar

{"title":"Genetic variations and interspesific relationships in Lonicera L. (Caprifoliaceae), using SCoT molecular markers","authors":"Fengzhen Chen, Dongmei Li, M. Farshadfar","doi":"10.36253/caryologia-1320","DOIUrl":"https://doi.org/10.36253/caryologia-1320","url":null,"abstract":"Lonicera L. (Caprifoliaceae) includes more than 200 species worldwide. The genus is mainly distributed in temperate to subtropical regions of the northern hemisphere: Europe, Russia, East Asia and North America. Some species are medicinal plants. Dried Lonicera flowers and buds are known as Flos Lonicera and have been a recognized herb in the traditional Chinese medicine for more than 1500 years. It has been applied for treatment of arthritis, diabetes mellitus, fever, and viral infections. Due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 85 randomly collected plants from six species in 6 provinces. Amplification of genomic DNA using 10 primers produced 103 bands, of which 95 were polymorphic (90.98%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Lonicera species. The genetic similarities of 6 collections were estimated from 0.67 to 0.90. According to the SCoT markers analysis, L. hypoleuca and L. iberica had the lowest similarity and the species of L. korolkowii and L. nummulariifolia had the highest similarity. The aims of present study are: 1) can SCoT markers identify Lonicera species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that SCoT markers can identify the species. ","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"5 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87307633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Palynological analysis of genus Geranium (Geraniaceae) and its systematic implications using scanning electron microscopy 天竺葵属(天竺葵科)孢粉分析及其系统意义

IF 2.1 4区生物学

Caryologia Pub Date : 2021-12-21 DOI: 10.36253/caryologia-1109

Jun Wang, C. Ye, Tong Zhang, Xusheng Shi, M. Khayatnezhad, A. Shakoor

{"title":"Palynological analysis of genus Geranium (Geraniaceae) and its systematic implications using scanning electron microscopy","authors":"Jun Wang, C. Ye, Tong Zhang, Xusheng Shi, M. Khayatnezhad, A. Shakoor","doi":"10.36253/caryologia-1109","DOIUrl":"https://doi.org/10.36253/caryologia-1109","url":null,"abstract":"Pollen morphology of 23 species belonging to Geranium have been studied in details, which represent eight sections of two subgenera i.e., G. sect. Dissecta, Geranium, and Tuberosa of subgen. Geranium, Divaricata, Lucida, Ruberta and Trilopha of subgen. Robertium. These plant species were collected from different phytogeographical regions of Iran. The palynological investigation was done using scanning electron microscopy (SEM) techniques. Different palyno-morphological features have been observed, and the closely related species were distinguished. We used different multivariate statistical methods to reveal the species relationships. Ward clustering analyses have been done to check out the relationship among the species. The shapes of pollen grains were monad, radially symmetric, isopolar, apertures were tricolporate, and of spheroid, prolate-spheroid or sub-prolate classes. Three pollen types were recognized on the basis of differences in exine sculpturing pattern: reticulate-clavate, striate-rugulate, reticulum cristatum with clavae. Observed differences were not of diagnostic importance in subgenera and sections level. The main objective of this study is to find distinguish pollen characters in the species of the genus Geranium and to elucidate their systematics importance.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"65 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77014166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic variations and interspesific relationships in Salvia (Lamiaceae) using SCoT molecular markers 利用SCoT分子标记分析鼠尾草属植物的遗传变异及种间亲缘关系

IF 2.1 4区生物学

Caryologia Pub Date : 2021-12-21 DOI: 10.36253/caryologia-886

Songpo Liu, Yuxuan Wang, Yuwei Song, M. Khayatnezhad, A. Minaeifar

{"title":"Genetic variations and interspesific relationships in Salvia (Lamiaceae) using SCoT molecular markers","authors":"Songpo Liu, Yuxuan Wang, Yuwei Song, M. Khayatnezhad, A. Minaeifar","doi":"10.36253/caryologia-886","DOIUrl":"https://doi.org/10.36253/caryologia-886","url":null,"abstract":"The genus Salvia includes an enormous assemblage of nearly 1000 species dispersed around the World. Iran having 19 endemic species out of 61 is regarded as one of the important regions for Salvia diversity in Southwest Asia. Salvia species are herbaceous, rarely biennial or annual, often strongly aromatic. These species are of medicinal, commercial and horticultural value. Due to the importance of these plant species, we performed a combination of morphological and molecular data for this species. For this study, we used 145 randomly collected plants from 30 species in 18 provinces. Amplification of genomic DNA using 10 primers produced 134 bands, of which 129 were polymorphic (97.78%). The obtained high average PIC and MI values revealed high capacity of SCoT primers to detect polymorphic loci among Salvia species. The genetic similarities of 30 collections were estimated from 0.61 to 0.93. According to the SCoT markers analysis, S. tebesana and S. verticillata had the lowest similarity and the species of S. eremophila and S. santolinifolia had the highest similarity. The aims of present study are: 1) can SCoT markers identify Salvia species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that SCoT markers can identify the species.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"4 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88841301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a protocol for genetic transformation of Malus spp 苹果属植物遗传转化方案的建立

IF 2.1 4区生物学

Caryologia Pub Date : 2021-12-21 DOI: 10.36253/caryologia-1248

F. Martinelli, A. Perrone, A. Dandekar

{"title":"Development of a protocol for genetic transformation of Malus spp","authors":"F. Martinelli, A. Perrone, A. Dandekar","doi":"10.36253/caryologia-1248","DOIUrl":"https://doi.org/10.36253/caryologia-1248","url":null,"abstract":"A protocol to produce transgenic shoots of Malus X domestica cv Greensleaves was optimized using two gene constructs previously used to create parthenocarpic tomato, Ino-IaaM and DefH9-IaaM. The aim was to obtain sufficient nº of transgenic shoots for in vitro multiplication, transfer to soil, grafting and testing for parthenocarpy in the next years. We investigated the effects of two modifications of a previous published protocol: 1) co-transformation with an Agrobacterium containing “VIP” genes in the gene construct and 2) two different hormones or hormone combinations. More shoot regeneration was obtained with a combination of three hormones (BA:NAA:TDZ) during co-cultivation instead of IBA and no co-transformation was performed using the VIP gene. For the DefH9-IaaM transgene, 21.04% regeneration was achieved for this treatment instead of 8.95% achieved with “IBA treatment” and 4.42% with the Agrobacterium co-transformation treatment. More shoot regeneration occurred with the combination of three hormones (BA:NAA:TDZ) instead of with only IBA and no co-transformation was performed using VIP gene. Experiments using Ino-IaaM confirmed the results shown for the DefH9-IaaM transgene. The regenerated shoots were multiplied in selective media containing kanamycin and roots were obtained. ","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"220 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75731710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0