Caryologia最新文献_第10页

Genetic (SSRs) versus morphological differentiation of date palm cultivars: Fst versus Pst estimates 枣椰树品种的遗传(SSRs)与形态分化:Fst与Pst的比较

IF 2.1 4区生物学

Caryologia Pub Date : 2021-11-26 DOI: 10.36253/caryologia-1089

S. Saboori, M. Sheidai, Zahra Noourmohammadi, S. Marashi, F. Koohdar

{"title":"Genetic (SSRs) versus morphological differentiation of date palm cultivars: Fst versus Pst estimates","authors":"S. Saboori, M. Sheidai, Zahra Noourmohammadi, S. Marashi, F. Koohdar","doi":"10.36253/caryologia-1089","DOIUrl":"https://doi.org/10.36253/caryologia-1089","url":null,"abstract":"Date Palm (Phoenix dactylifera L.) is one of the oldest domesticated fruit trees. For future breeding program, knowledge on genetic structure of cultivars is necessary. Therefore, the present study was performed with the following aims: 1- To provide data on genetic diversity and genetic structure of 36 date palm cultivars, 2- To provide data on the association between fruit characteristics and the genetic features of the cultivars. We used nine SSRs and EST-SSR loci for our genetic investigation. The most of SSR loci obtained have a high Gst value (0.70), and therefore have a good discrimination power for date palm cultivar differentiation task. K-Means clustering grouped date palm cultivars either in two broad clusters, or in 16 smaller genetic groups. This was supported by delta K = 2 of the STRUCTURE analysis. AMOVA produced significant genetic difference among date palm cultivars (PhiPT = 0.70, P = 0.001). New genetic differentiation parameters estimated also produced significant difference among date palm cultivars (G’st(Nei) = 0.673, P =0.001; G’st(Hed) = 0.738, P = 0.001). Test of assignment revealed that some of the cultivars have 33-66% misassignment, probably due to genetic admixture. Heatmaps of genetic versus morphological/or agronomical characters in date palm cultivars differed from each other showing the cultivars morphological changes is not merely related to their genetic content. It points toward the potential role played either by environmental conditions or local selection practice. The new findings can be utilized in future conservation and breeding of date palms in the country.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"26 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83566198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa 形态学方法和分子标记法确定了异食草的遗传多样性和种群结构

IF 2.1 4区生物学

Caryologia Pub Date : 2021-10-08 DOI: 10.36253/caryologia-958

K. Zhu, Lijie Liu, Shanshan Li, Bo Li, M. Khayatnezhad, Abdul Shakoor

{"title":"Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa","authors":"K. Zhu, Lijie Liu, Shanshan Li, Bo Li, M. Khayatnezhad, Abdul Shakoor","doi":"10.36253/caryologia-958","DOIUrl":"https://doi.org/10.36253/caryologia-958","url":null,"abstract":"The Caryophyllaceae family is complex. Several attempts have been carried out in the past to study Caryophyllaceae members. This study mainly focused on Allochrusa Bunge to determine its genetic structure and used ISSR markers, ITS, and rps16 data to classify and differentiate Allochrusa species. We collected 122 Allochrusa specimens. Our analysis included morphological and molecular method approaches. Morphometry analysis indicated that floral characters could assist in the identification of Allochrusa species. A. persica (Boiss.) Boiss. and A. versicolor Fisch. & C.A.Mey. showed affinity to each other. A. bungei Boiss. formed a separate group. Analysis of molecular variance showed significant genetic differentiation in Allochrusa (p= 0.001). The majority of genetic variation was among the Allochrusa population. We recorded minimum gene flow (Nm=0.176) between Allochrusa species. Besides this, isolation by distance occurs in Allochrusa members, as shown in the Mantel test result (r = 0.01, p = 0.0002). STRUCTURE analysis revealed three genetic groups. It is evident that A. persica, A. versicolor, and A. bungei differ genetically from each other. Our current findings have implications in plant systematics and biodiversity management.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"59 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80931682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species 柽柳遗传多样性及基因组DNA提取方案的比较研究

IF 2.1 4区生物学

Caryologia Pub Date : 2021-10-08 DOI: 10.36253/caryologia-1056

Xiao Cheng, Xiaoling Hong, M. Khayatnezhad, F. Ullah

{"title":"Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species","authors":"Xiao Cheng, Xiaoling Hong, M. Khayatnezhad, F. Ullah","doi":"10.36253/caryologia-1056","DOIUrl":"https://doi.org/10.36253/caryologia-1056","url":null,"abstract":"The genus Tamarix consists of about 54 species that mainly grow in saline areas of deserts and semi-deserts. This genus is chemically characterized by the presence of tannins, flavonoids, anthocyanins and essential oils which interfere with the extraction of pure genomic DNA. Thus it is necessary to optimize extraction protocols to minimize the influence of these compounds to the lowest level. The present study compares the efficiency of five different approaches to extract total genomic DNA in Tamarix species, showing significant differences in the extracted DNA contents and quality,by using Kit (DNP TM Kit), CTAB DNA extraction method by Murray and Thompson, Sahu et al., Nalini et al. and Bi et al., for the extraction of DNA from Tamarix species. Our results showed significant differences in DNA contents between these five methods. The quantity and quality of extracted genomic DNA were checked by the spectrophotometer, Nano-Drop and and agarose gel electrophoresis analysis. Finally, a PCR-based method was also applied to verify the amplification efficiency for two molecular markers (ITS and ISSR).. In the present study, the genetic diversity of 96 Tamarix individuals species and 8 populations were studied using 10 ISSR markerswhile for nrDNA ITS 8 species samples were used. The method of Nalini et al., provided best results (207 ng/μL) in terms of quantity and quality ofDNA. Our results proposed that this method could be effective for plants with the same polysaccharides, proteins and polyphenols components. The advantage of this method is simple and fast as it does not involve time consuming steps such as incubation at higher temperatures, and also do not requires expensive chemicals such as proteinase K, liquid nitrogen. ,. The success of this method in obtaining high-quality genomic DNA has been demonstrated in the Tamarix species group and the reliability of this method has been discussed.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"98 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77198249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

First genome size assessments for Marshallia and Balduina (Asteraceae, Helenieae) reveal significant cytotype diversity 首次基因组大小评估显示marshall和Balduina (Asteraceae, Helenieae)具有显著的细胞型多样性

IF 2.1 4区生物学

Caryologia Pub Date : 2021-08-22 DOI: 10.36253/caryologia-1206

T. Garnatje, J. Pellicer, J. Vallès, N. Hall, C. Hansen, L. Goertzen

引用次数: 0

Comparative study of genetic diversity using RAPD markers in Salvia (Lamiaceae) 基于RAPD标记的鼠尾草科植物遗传多样性比较研究

IF 2.1 4区生物学

Caryologia Pub Date : 2021-08-17 DOI: 10.36253/caryologia-1236

Majid Khayyatnezhad, S. Shah

{"title":"Comparative study of genetic diversity using RAPD markers in Salvia (Lamiaceae)","authors":"Majid Khayyatnezhad, S. Shah","doi":"10.36253/caryologia-1236","DOIUrl":"https://doi.org/10.36253/caryologia-1236","url":null,"abstract":"Salvia has a high degree of environmental compatibility and is widespread around the world, especially in tropical and temperate regions. It is represented by 61 species in Iran including 19 endemic species. Salvia species are mostly shrubs or subshrubs, occasionally herbs, typically perennial, sometimes biennial or annual, and often aromatic. The genus has high medicinal, commercial and horticultural value. It is the largest and one of the taxonomically complicated genus of Lamiaceae. To determine the genetic diversity and understand the species’ limits within the Iranian Salvia, we produced both morphological and molecular data using 145 randomly collected plants representing 30 species from 18 provinces of Iran. A total of 107 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 10.7 bands/primer and 44% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. reuterana. Our data depicted highest similarity between S. suffruticosa and S. hydrangea and lowest between S. aristata and S. oligphylla. Salvia limbata showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"13 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79166604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of CMA-DAPI bands and preparation of fluorescent karyotypes in thirty Indian cultivars of Lens culinaris 30个印度稻属品种CMA-DAPI谱带分析及荧光核型制备

IF 2.1 4区生物学

Caryologia Pub Date : 2021-08-16 DOI: 10.36253/caryologia-919

T. Jha, B. Bhowmick, P. Roy

{"title":"Analysis of CMA-DAPI bands and preparation of fluorescent karyotypes in thirty Indian cultivars of Lens culinaris","authors":"T. Jha, B. Bhowmick, P. Roy","doi":"10.36253/caryologia-919","DOIUrl":"https://doi.org/10.36253/caryologia-919","url":null,"abstract":"India holds a significant rank in production and consumption of the age old protein rich crop Lentil with only one cultivated species and a large number of phenotypically similar cultivars. The need for a reliable and cost effective method of genetic characterization to unravel differences within the Lentil cultivars was felt. The present paper adopted EMA based chromosome preparation followed by staining with two contrasting fluorochromes dyes CMA and DAPI that bind directly to GC and AT rich heterochromatic segments on chromosomes. Analysis of fluorochrome banding pattern furnished a comparative account of genetic diversity within the cultivars that could not be achieved by traditional karyotyping. The marker pair of nucleolar chromosomes (4th and 3rd, majorly) occupied a pivotal position to intensify differences between cultivars in terms of banding patterns around secondary constrictions, suggestive of yet unknown variation in heterochromatin composition. Our study has strengthened genetic background and relationships of Lentil cultivars. We observed certain types of unusual fluorochrome bands that put forward the exclusivity of Indian germplasm and have questioned the mainstream heterochromatin elements of plant chromosomes captured by CMA-DAPI stains. The comprehensive fluorescent karyotypes of 30 L. culinaris cultivars prepared for the first time, serve as an archetype for the benefit of future breeding programmes in any Indian crop. ","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"68 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74614357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mutagenic and Cytotoxic Activity of Insecticide Napoleon 4EC in Allium cepa and Ames Test 杀虫剂拿破仑4EC对葱的致突变性和细胞毒活性及Ames试验

IF 2.1 4区生物学

Caryologia Pub Date : 2021-08-16 DOI: 10.36253/caryologia-1013

D. Akyıl

{"title":"Mutagenic and Cytotoxic Activity of Insecticide Napoleon 4EC in Allium cepa and Ames Test","authors":"D. Akyıl","doi":"10.36253/caryologia-1013","DOIUrl":"https://doi.org/10.36253/caryologia-1013","url":null,"abstract":"The objective of this study was to explore the mutagenic and cytotoxic effects of Napoleon 4EC pesticide used in Turkey to control insect pest by using two standard assays. The Allium cepa test was used for determined the cytotoxic effects of this pesticide. For this test, onion seeds were exposed to Napoleon 4EC (100, 200, and 400 ppm) for 24, 48, and 72 hours. For each test group root tip cells were stained with Feulgen and five slides were prepared for each concentration and counted microscopically. The concentrations Napoleon 4EC was compared with the value for the negative control using Dunnet-t test, 2 sided. The results indicated that mitotic index was clearly decreased with increasing the concentration of Napoleon 4EC in each treatment group as compared to the controls. The percentage of mitotic phases has been markedly impacted. Five different doses of the pesticide (50, 100, 200, 400, 800 μg/plate) were examined with Ames test using Salmonella typhimurium strains TA98 and TA100 with and without S9 metabolic activation for mutagenic activity. Ames test results showed a dose dependent effect, but not twice the negative control for S. typhimurium TA98 and TA100, with or without S9 mix except 800 μg/plate doses. In 800 μg/plate doses, colony numbers are two-fold increase according to colony number of control group. So, this places the this compound as a weak mutagen according to the parameters.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"71 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75656411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic and Genotoxic Effects of Methanol Extracts of Some Gypsophila L. Species 部分Gypsophila L.种甲醇提取物的细胞毒性和基因毒性作用

IF 2.1 4区生物学

Caryologia Pub Date : 2021-08-09 DOI: 10.36253/caryologia-1091

Ferhan Korkmaz, Özgün Tuna Gülören, Meltem Erdi̇r, E. Ataşlar

引用次数: 0

Comparative cytogenetics in three species of Wood-Warblers (Aves: Passeriformes: Parulidae) reveal divergent banding patterns and chromatic heterogeneity for the W chromosome 三种林莺(雀鸟:雀形目:林莺科)的细胞遗传学比较揭示了其W染色体的不同带型和颜色异质性

IF 2.1 4区生物学

Caryologia Pub Date : 2021-07-20 DOI: 10.36253/CARYOLOGIA-839

A. Costa, Cassiane Furlan Lopes, Marcelo Santos de Souza, S. A. Barcellos, Pâmela Giordani Vielmo, R. J. Gunski, A. D. Garnero

{"title":"Comparative cytogenetics in three species of Wood-Warblers (Aves: Passeriformes: Parulidae) reveal divergent banding patterns and chromatic heterogeneity for the W chromosome","authors":"A. Costa, Cassiane Furlan Lopes, Marcelo Santos de Souza, S. A. Barcellos, Pâmela Giordani Vielmo, R. J. Gunski, A. D. Garnero","doi":"10.36253/CARYOLOGIA-839","DOIUrl":"https://doi.org/10.36253/CARYOLOGIA-839","url":null,"abstract":"Chromosomal rearrangements are an important process in the evolution of species. It is assumed that these rearrangements occur near repetitive sequences and heterochromatic regions. Avian karyotypes have diverse chromosomal band patterns and have been used as the parameters for phylogenetic studies. Although the group has a high diversity of species, no more than 12% has been analyzed cytogenetically, and the Parulidae family are extremely underrepresented in these studies. The aim of this study was to detect independent or simultaneous chromosomal rearrangements, and also to analyze chromosomal banding convergences and divergences of three Wood-Warblers species (Myiothlypis leucoblephara, Basileuterus culicivorus, and Setophaga pitiayumi). Our CBG-band results reveal an unusual W sex chromosome in the three studied species, containing a telomeric euchromatic region. The GTG and RBG bands identify specific regions in the macrochromosomes involved in the rearrangements. Cytogenetic data confirm the identification of speciation processes at the karyotypic of this group.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"17 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87340642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ploidy level determination of Hedera (Araliaceae) with an emphasis on discussable species (Hedera hibernica) 五缘木属植物倍性水平的测定——以可讨论种为重点

IF 2.1 4区生物学

Caryologia Pub Date : 2021-07-20 DOI: 10.36253/CARYOLOGIA-881

Fahimeh Fallah, F. Ghahremaninejad

引用次数: 1