Morphological method and molecular marker determine genetic diversity and population structure in Allochrusa

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2021-10-08 DOI:10.36253/caryologia-958

K. Zhu, Lijie Liu, Shanshan Li, Bo Li, M. Khayatnezhad, Abdul Shakoor

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引用次数: 14

Abstract

The Caryophyllaceae family is complex. Several attempts have been carried out in the past to study Caryophyllaceae members. This study mainly focused on Allochrusa Bunge to determine its genetic structure and used ISSR markers, ITS, and rps16 data to classify and differentiate Allochrusa species. We collected 122 Allochrusa specimens. Our analysis included morphological and molecular method approaches. Morphometry analysis indicated that floral characters could assist in the identification of Allochrusa species. A. persica (Boiss.) Boiss. and A. versicolor Fisch. & C.A.Mey. showed affinity to each other. A. bungei Boiss. formed a separate group. Analysis of molecular variance showed significant genetic differentiation in Allochrusa (p= 0.001). The majority of genetic variation was among the Allochrusa population. We recorded minimum gene flow (Nm=0.176) between Allochrusa species. Besides this, isolation by distance occurs in Allochrusa members, as shown in the Mantel test result (r = 0.01, p = 0.0002). STRUCTURE analysis revealed three genetic groups. It is evident that A. persica, A. versicolor, and A. bungei differ genetically from each other. Our current findings have implications in plant systematics and biodiversity management.

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形态学方法和分子标记法确定了异食草的遗传多样性和种群结构

石竹科植物很复杂。过去曾多次尝试对石竹科成员进行研究。本研究主要以异食石(Allochrusa Bunge)为研究对象，确定其遗传结构，并利用ISSR标记、its和rps16数据对异食石进行分类和区分。我们收集了122只异齿兽标本。我们的分析包括形态学和分子方法。形态计量学分析表明，花系性状可以作为异花楸属植物的鉴别依据。波斯卡(波斯)木香。和杂色鱼。& C.A.Mey。表现出彼此的亲和力。龙葵。组成了一个独立的小组。分子变异分析显示，异食豆的遗传分化显著(p= 0.001)。遗传变异主要发生在异食兽种群中。结果表明，不同种间的基因流量最小，Nm=0.176。此外，距离隔离在Allochrusa成员中发生，如Mantel试验结果(r = 0.01, p = 0.0002)所示。结构分析显示三个遗传群。木香、花斑木香和白蜡木香在遗传上存在明显差异。本研究结果对植物分类学和生物多样性管理具有指导意义。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.