IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2430

Vivek Singh

引用次数: 0

Role of ten different exogenous plant growth promoters in regulating cytotoxic and genotoxic processes in barley exposed to high temperature stress 十种不同的外源植物生长促进剂在调节暴露于高温胁迫的大麦的细胞毒性和基因毒性过程中的作用

IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2409

S. Tabur, Ş. B. Yilmaz Ergün, Serkan Özmen

{"title":"Role of ten different exogenous plant growth promoters in regulating cytotoxic and genotoxic processes in barley exposed to high temperature stress","authors":"S. Tabur, Ş. B. Yilmaz Ergün, Serkan Özmen","doi":"10.36253/caryologia-2409","DOIUrl":"https://doi.org/10.36253/caryologia-2409","url":null,"abstract":"Agricultural crop affected preliminary and the most prominent by the adverse effects of global climate change have to adapt to various abiotic factors that will occur as a result of climate fluctuations in the near-future and struggle to survive. Among abiotic factors, the one of the greatest impact on plant stress is high temperature. Therefore, the most important step to take action against the global threat is the development of new temperature tolerant varieties. Barley, which is the fourth most important cereal in the world after wheat, maize, and rice are affected by high ambient temperatures. In this work, the effects of their alone and in double, triple combinations of ten various plant growth regulators (PGRs) on mitotic activity and chromosome behaviors in root meristems of barley exposed to high temperature (30°C) were investigated. In the experiments, Hordeum vulgare L. cv. Bülbül 89 variety and GA3, KIN, BA, E, EBR, TRIA and PAs (Spm, Spd, Put, Cad) as growth regulators were used. The results obtained were compared with each other and with those of the seeds germinated at optimum temperature (20°C). Consequently; it has been determined that most of the PGRs studied, especially the GA3 and their combinations with GA3, exhibit a very successful performance on mitotic activity and cytogenetic aberrations in barley seeds germinated under high temperature stress- HTS conditions. The effects of these PGRs (except for EBR) and their combinations on mitotic activity and chromosome behaviors under HTS have been presented in this study for the first time.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141669933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological, genetical changes and cdc2 gene expression for osmotic stressed Vicia faba reveal the alleviation effect of gamma radiation and putrescine 渗透压胁迫下紫花苜蓿的生理、基因变化和 cdc2 基因表达揭示了伽马射线和腐胺的缓解作用

IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2338

Shaimaa S. Sobieh, Noha Eid Eliwa

{"title":"Physiological, genetical changes and cdc2 gene expression for osmotic stressed Vicia faba reveal the alleviation effect of gamma radiation and putrescine","authors":"Shaimaa S. Sobieh, Noha Eid Eliwa","doi":"10.36253/caryologia-2338","DOIUrl":"https://doi.org/10.36253/caryologia-2338","url":null,"abstract":"Climate change caused increasing in soil salinity worldwide. Therefore, it is critical to enhance the capability of plant to tolerate salinity stress. For this goal, putrescine and irradiation by gamma radiation were used to improve the salt tolerance of Vicia faba (the most important human crop). The results indicated depression in mitotic division and all growth parameters associated with the induction of micronucleus (MN) when salinity increased to 100mM, while there was increase in mitotic aberrations. NaCl decreased total soluble sugar, while total N%, total free amino acid, proline and protein contents showed slight increase with increasing salinity stress. Putrescine and gamma radiation mitigated the effect of salinity on cell division and growth parameters. Salt stress decreased the expression of gene encoding cyclin-dependent kinase 2 (cdc2). Both putrescine and gamma radiation increased cdc2 expression. The genetic diversity has been detected among control and treated V. faba using ISSR and SCoT markers. Ten primers had successfully generated 129 reproducible polymorphic amplicons that were suitable for studying the genetic diversity between studied genotypes. ISSR markers provided more discriminating data and were more informative than SCoT markers. Besides, cluster analysis using UPGMA and PAC successfully explained the genetic diversity within studied genotypes. These findings emphasize the efficiency of putrescine and gamma radiation for alleviating the negative impact of salt stress. Moreover, prove the importance of assessing mitotic activity, chromosomes behavior, physiological parameters, the expression level of cdc2 and molecular diversity in V. faba under stress to improve the salt tolerance of it.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meiotic behavior during microsporogenesis, responsible for male sterility in some species of Salvia sect. Aethiopis in Iran 小孢子发生过程中的减数分裂行为是造成伊朗丹参科某些物种雄性不育的原因。伊朗的 Aethiopis

IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2288

Fatemeh Hajmoradi

{"title":"Meiotic behavior during microsporogenesis, responsible for male sterility in some species of Salvia sect. Aethiopis in Iran","authors":"Fatemeh Hajmoradi","doi":"10.36253/caryologia-2288","DOIUrl":"https://doi.org/10.36253/caryologia-2288","url":null,"abstract":"The genus Salvia L. (Lamiaceae: tribe Mentheae) contains about 1000 species. Section Aethiopis in this genus has a distribution in the Mediterranean and central Asia and contains about 34 species in Iran. This study aimed to investigate the chromosome number and meiotic behavior in five species of Salvia sect. Aethiopis. To this end, pollen mother cells were used, and the squash method was employed. The results showed that S. persepolitana and S. spinosa had a chromosome number of 2n=2x=20, while S. sclarea, S. hypoleuca, and S. limbata had a chromosome number of 2n=2x=22. The study of meiotic behavior revealed the presence of abnormalities such as chromosome stickiness, cytomixis, non-synchronous segregation, chromosome bridges, laggard chromosomes, formation of micronuclei in tetrad cells, formation of tripolar cells, and pentapolar with different frequencies in the studied species. The meiotic index was reported as the highest in S. persepolitana and the lowest in S. hypoleuca. Pollen fertility was also affected by meiotic abnormalities, showing a decreasing trend from S. persepolitana to S. spinose. Meiotic abnormalities may have played a role in the evolution of aneuploidy and polyploidy in the Salvia genus.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial power of Pomegranate extracts against Beta-Lactamase producing Escherichia coli 石榴提取物对产β-内酰胺酶大肠杆菌的抗菌能力

IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2356

Mashail A. Alghamdi, F. Al-Sarraj, Wafa H. Alamshani, I. Alotibi, Majid Al-Zahrani, R. Albiheyri, Nada M. Nass, B. Sajer, Noor M. Bataweel, Mohammed A. Al-Matary, Thamer A. Bouback, Dalya Attallah, Tahani M. Alqahtani

{"title":"Antibacterial power of Pomegranate extracts against Beta-Lactamase producing Escherichia coli","authors":"Mashail A. Alghamdi, F. Al-Sarraj, Wafa H. Alamshani, I. Alotibi, Majid Al-Zahrani, R. Albiheyri, Nada M. Nass, B. Sajer, Noor M. Bataweel, Mohammed A. Al-Matary, Thamer A. Bouback, Dalya Attallah, Tahani M. Alqahtani","doi":"10.36253/caryologia-2356","DOIUrl":"https://doi.org/10.36253/caryologia-2356","url":null,"abstract":"Herbs, as the pomegranate (Punica granatum L.)(P. granatum), has significant chemical constituents with distinct pharmacological properties. These chemicals confer neuroprotective, antioxidant, anticancer, anti-inflammatory, and antibacterial properties to the plant. Pomegranate has specific components that enable its pharmacological actions; one of the functions of pomegranate extracts is to deactivate what is called extended spectrum beta-lactamase (ESBL) that makes Escherichia coli (E. coli) resistant to standard antibiotics. Twenty E. coli strains identified as beta-lactamase producers, the strains isolated from urine samples collected from patients with symptomatic urinary tract infection (UTI) and molecularly characterised using 16S rDNA. The study evaluated the antibiotic sensitivity and antibacterial activities of acetone and ethanolic pomegranate leaf and peel extracts, assessing their antimicrobial susceptibility against nineteen antibiotics. The ehanolic peel (EP) and leave (EL) extracts showed inhibitory potential inhibition zones spanning (9.0-12.6-18.3 mm) against E. coli pathogen producing extended-spectrum beta-lactamase as compared with (10.2-15.3 mm) inhibition scale exhibited by acetone peel (AP) and leave (AL) extracts treatment. Pomegranate leaves and peel extracts contain bioactive compounds with antioxidant, antimicrobial, and other biological effects, and can be fractionated for the identification of new antibacterial bioactive compounds for the development of drugs against ESBL- E. coli, in addition to their synergy with antibiotics for combination therapy that may have effective management and treatment of multidrug-resistant infections such as urinary tract infection.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyological studies of four species of Lady’s Slipper Orchids (Paphiopedilum) collected in the Bogor Botanical Garden, Indonesia 印度尼西亚茂物植物园采集的四种瓢唇形兰花（Paphopedilum）的核果学研究

IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2232

T. N. Praptosuwiryo, R. Garvita, Elizabeth Handini, I. Fijridiyanto, J. Witono

{"title":"Karyological studies of four species of Lady’s Slipper Orchids (Paphiopedilum) collected in the Bogor Botanical Garden, Indonesia","authors":"T. N. Praptosuwiryo, R. Garvita, Elizabeth Handini, I. Fijridiyanto, J. Witono","doi":"10.36253/caryologia-2232","DOIUrl":"https://doi.org/10.36253/caryologia-2232","url":null,"abstract":"Paphiopedilum is one of the most widely grown and hybridized of all orchid genera due to its distinctive flower morphology. This genus consists of 139 accepted species and is native to southern China to tropical Asia. Karyological studies on the genus Paphiopedilum have been reported by many cytologists in different countries. However, many Indonesian members of Paphiopedilum have remained comparatively limited in investigated cytologically. This study aimed to analyze karyological characters of four species of Paphiopedilum collected in the Bogor Botanical Garden, Indonesia, namely P. armeniacum, P. hirsutissimum, P. primulinum, and P. superbiens. Karyological studies were conducted by root tips squash method. The results showed that four species of Paphiopedilum have a basic chromosome number of x = 15. They are diploid with 2n=30, instead of the common diploid chromosome number of Paphiopedilum (2n = 26). Paphiopedilum hirsutissimum and P. primulinum possess 16 m + 14 sm chromosome formulae. Whereas Paphiopedilum armeniacum and P. superbiens revealed 16 m + 14 sm and 18 m + 12 sm chromosome formulae, respectively.A Robertsonian change in chromosome number generated by the fission of chromosomes would best explain the origin of the new diploid chromosome number and karyotypes of these species.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytotoxicity, cytogenotoxicity and antimicrobial potential of extracts with gold-silver bimetallic nanoparticles obtained from pteridophyte spores 从翼手目植物孢子中提取的金银双金属纳米颗粒的植物毒性、细胞毒性和抗菌潜力

IF 2.1 4区生物学

Caryologia Pub Date : 2024-07-08 DOI: 10.36253/caryologia-2424

Oana Alexandra Luțu, L. Soare, I. Fierăscu, R. Fierăscu, Codruța Mihaela Dobrescu, Alina Păunescu, C. Ponepal, C. Topală, L. Vîjan, I. Deliu, Aurelian Denis Negrea, D. Vîlcoci, Georgiana Cîrstea, Florentina Aldea, S. Honțaru, A. Șuțan

{"title":"Phytotoxicity, cytogenotoxicity and antimicrobial potential of extracts with gold-silver bimetallic nanoparticles obtained from pteridophyte spores","authors":"Oana Alexandra Luțu, L. Soare, I. Fierăscu, R. Fierăscu, Codruța Mihaela Dobrescu, Alina Păunescu, C. Ponepal, C. Topală, L. Vîjan, I. Deliu, Aurelian Denis Negrea, D. Vîlcoci, Georgiana Cîrstea, Florentina Aldea, S. Honțaru, A. Șuțan","doi":"10.36253/caryologia-2424","DOIUrl":"https://doi.org/10.36253/caryologia-2424","url":null,"abstract":"Investigating the toxicity of naturally occurring or synthesized nanoparticles for various applications is absolutely necessary for environmental protection and safety use. The aim of these research was to investigated the phytotoxicity, cytogenotoxicity and antibacterial potential of the extracts with gold-silver bimetallic nanoparticles (Au-Ag NPs) obtained from green synthesis in Asplenium scolopendrium L. and Dryopteris filix-mas (L.) Schott spores extracts. To our knowledge, this is the first report of the Au-Ag NPs phytosynthesis based on extracts obtained from fern spores. UV-Vis spectroscopy analysis of the samples revealed the maximum absorbance, characteristic of samples with bimetallic nanoparticles, which varied depending on the Au:Ag ratio. Energy-dispersive X-ray spectroscopy confirmed the presence and distribution of Au, Ag and other chemical elements. The presence of specific secondary metabolites in the extracts that helped in NPs biosynthesis stimulated growth processes. Good results were recorded for some Dryopteris filix-mas samples, correlated with a significantly increased mitotic index. Cell viability decreased significantly in three of the nanoformulations. Only extracts with Au-Ag NPs showed antimicrobial effect against Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633 and Escherichia coli ATCC 8739. The testing of the antibacterial potential of these extracts must be extended to other bacterial strains and other microorganisms, the search of new antimicrobial resources being an urgent necessity nowadays.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141669990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytogenotoxic and antimicrobial effects of Nezara viridula (L.) (Hemiptera: Heteroptera: Pentatomidae) alcoholic extracts Nezara viridula (L.) (Hemiptera: Heteroptera: Pentatomidae) 酒精提取物的细胞毒性和抗菌作用

IF 2.1 4区生物学

Caryologia Pub Date : 2024-03-15 DOI: 10.36253/caryologia-2389

N. A. Şuțan, M. Bărbuceanu, Daniela Barbuceanu, I. Deliu

引用次数: 0

First cytogenetic study of the Somphong’s rasbora (Trigonostigma somphongsi) (Perciformes, Cyprinidae), a critically endangered species in Thailand 泰国极度濒危物种 Somphong's rasbora（Trigonostigma somphongsi）（鲈形目，鲤科）的首次细胞遗传学研究

IF 2.1 4区生物学

Caryologia Pub Date : 2024-03-15 DOI: 10.36253/caryologia-2439

S. Aiumsumang, C. Vidthayanon, S. Kulabtong, A. Tanomtong, S. Phimphan

引用次数: 0

Karyological analyses in several Algerian populations of six species of the genus Vicia L. (Fabaceae) 对阿尔及利亚六个禾本科植物（豆科）种群的核子学分析

IF 2.1 4区生物学

Caryologia Pub Date : 2024-03-15 DOI: 10.36253/caryologia-1924

Zahia Sebkhi, R. Issolah, Nabila Melzi, Hassina Benmouhoub, Mohamed Mefti

{"title":"Karyological analyses in several Algerian populations of six species of the genus Vicia L. (Fabaceae)","authors":"Zahia Sebkhi, R. Issolah, Nabila Melzi, Hassina Benmouhoub, Mohamed Mefti","doi":"10.36253/caryologia-1924","DOIUrl":"https://doi.org/10.36253/caryologia-1924","url":null,"abstract":"As part of the evaluation and valorization of plant genetic resources of fodder and pastoral interest in Algeria, seventeen (17) natural populations belonging to six (06) species of the genus Vicia (Vicia sativa, Vicia disperma, Vicia monardii, Vicia ohchroleuca, Vicia onobrychioides and Vicia lutea), originated from different ecological regions in the North-Eastern of the country, were considered. The populations have been the subject of mitotic and meiotic studies. Haploid and diploid numbers and chromosome measurements were determined. Original results were observed for the first time in some species. In fact, chromosome counts have revealed some new chromosome numbers. The first number (2n=14, n=7) was observed in the endemic subspecies of Algeria, V. ochroleuca subsp. atlantica and in the species Vicia onobrychioides. The second number (n=6) was observed in the species Vicia disperma. Within Vicia monardii, the three new chromosome numbers, previously observed only in mitosis by our research team, were confirmed for the first time through the present meiosis study (n=6, 7 and 8), indicating that they are A type chromosomes. The base number x=7 is the most frequently observed number in the six Vicia species. The observed chromosome numbers would be related to some ecological factors (altitude, rainfall) of the origin environment of the populations. Chromosome measurements and established karyotypes were determined for the first time in V. monardii, V. onobrychioides and V. ochroleuca subsp. atlantica. Chromosome size and karyotype formula are variable among the studied species and subspecies. Karyotypes vary from symmetrical to asymmetrical and the intrachromosomal asymmetry is higher than interchromosomal one. The new cytogenetic data would contribute to a better understanding of the evolution mechanism of the species in the genus Vicia L.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caryologia最新文献