Caryologia最新文献_第2页

Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L. 蒺藜对洋葱和蚕豆分生组织细胞的细胞遗传学影响。

4区生物学

Caryologia Pub Date : 2023-09-19 DOI: 10.36253/caryologia-2092

Ali Bouzekri, Meryem Nassar, Souheila Slimani, Zohra Chekroud

{"title":"Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.","authors":"Ali Bouzekri, Meryem Nassar, Souheila Slimani, Zohra Chekroud","doi":"10.36253/caryologia-2092","DOIUrl":"https://doi.org/10.36253/caryologia-2092","url":null,"abstract":"Tribulus terrestris is a plant of the Zygophyllaceae family frequently used worldwide to treat various diseases due to the therapeutic effects of its pharmacological components. This study examines the cytotoxic and genotoxic effects of T. terrestris using two plant models, Allium cepa and Vicia faba. Extracts of 0.00625, 0.0125, 0.025, 0.05 and 0.1mg/mL were tested on meristematic cells of A. cepa and V. faba roots. This assessment includes the study of root growth, structure and coloration, as well as the determination of the mitotic index (MI) and chromosomal aberrations (CAs) as accurate indicators of toxicity. Our results showed a significant decrease in the mean length of roots treated with 0.025, 0.05 and 0.1 mg/ml for A. cepa and 0.1 mg/ml for V. faba. Cytotoxicity and genotoxicity results showed a significant decrease in MI from 0.025 mg/ml in A. cepa and from 0.05 mg/ml in V. faba, and this decrease in MI is linked to the increase in concentration and treatment time with T. terrestris. Furthermore, a significant increase in CAs was observed in A. cepa and V. faba from the 0.025 mg/ml concentration. The significant reduction in MI and CAs abundance suggests the genotoxicity of T. terrestris. Therefore, T. terrestris is a medicinal plant that should be used with caution, appropriately and based on essential therapeutic needs.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135110197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karyomorphology, genome size, and variation of antioxidant in twelve berry species from Iran 伊朗12种浆果的核形态、基因组大小和抗氧化剂变异

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1633

Saeed Mohammadpour, G. Karimzadeh, S. Ghaffari

引用次数: 0

Studying some morphological responses of stevia (Stevia rebaudiana Bertoni) to some elicitors under water deficiency 研究了甜叶菊(stevia rebaudiana Bertoni)在缺水条件下对某些激发子的形态响应

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1922

Basoz Sadiq Muhealdin, S. Hamarashid, Fairuz Ibrahim Ali, N. Abdulla, Syamand Ahmad Qadir

{"title":"Studying some morphological responses of stevia (Stevia rebaudiana Bertoni) to some elicitors under water deficiency","authors":"Basoz Sadiq Muhealdin, S. Hamarashid, Fairuz Ibrahim Ali, N. Abdulla, Syamand Ahmad Qadir","doi":"10.36253/caryologia-1922","DOIUrl":"https://doi.org/10.36253/caryologia-1922","url":null,"abstract":"This research evaluated the effect of foliar spraying of different elicitors on modulating the effect of water stress on the stevia. The experimental design was the split-plot based on a randomized complete block design with three repetitions. Experimental treatments included different irrigation regimes (90% FC, 65% FC, and 40% FC) and foliar application of different elicitors (control, chitosan, salicylic acid, and melatonin). In this study, the content of chlorophyll A and b was reduced by intensifying the water deficit stress. Also, the highest content of the two pigments was allocated to the treatment of melatonin application. In the present study, melatonin foliar application under 90% FC irrigation conditions had the highest plant height, leaf area index, biomass, and carotenoid content, Moreover, the highest content of proline, phenol, DPPH, rebaudioside A, and steviosid was assigned to melatonin foliar application treatment under 40% FC irrigation conditions. Results revealed, although water stress reduced plant height, leaf area index, and plant biomass, the application of melatonin and salicylic acid under different irrigation conditions moderated the effect of water stress on these traits. Application of melatonin and salicylic acid under water deficit stress conditions also increased the content of proline, phenol, DPPH, rebaudioside, and steviosid.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"219 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79814728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the absorption ability of nitrate and lead by japanese raisin under salt stress conditions 盐胁迫条件下日本葡萄干对硝酸盐和铅的吸收能力评价

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1827

S. M. Hosseini, S. KalateJari, M. Kafi, B. Motesharezadeh

{"title":"Assessment of the absorption ability of nitrate and lead by japanese raisin under salt stress conditions","authors":"S. M. Hosseini, S. KalateJari, M. Kafi, B. Motesharezadeh","doi":"10.36253/caryologia-1827","DOIUrl":"https://doi.org/10.36253/caryologia-1827","url":null,"abstract":"Heavy metals pollution is an important challenge that was cussed by human activity, this stress decreases under salinity. The aim of this study was to investigate the ability of Japanese raisin in the absorption of nitrate (0, 30, and 60 mgL-1) and lead (0, 300, and 600 mgL-1) under salinity stress (0 as control and 3 and 6 dSm-1). Results showed that the studied plant continued to uptake nitrate and potassium under stress conditions of Pb and salinity. Although Na and Cl uptake were observed as a defense mechanism in the plant, the K/Na ratio, and K content increased from 1 to 6 and from 1.8 to 5%, respectively. Also, the most appropriate physiological responses were observed at treatments under contamination level of 300 mg Pb and salinity level of 3 dSm-1, so that the synthesis of malondialdehyde (MDA) and enzymatic activity increased at these levels of HMs and salinity. Based on the results, the studied species were able to uptake moderate concentrations of Pb (34.1-71mg kg-1) under experimental conditions. Hence, its potential for the clean-up of some contaminants in the environment can be considered by researchers for further research.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"11 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82298129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraspecific karyomorphological and genome size variations of in vitro embryo derived Iranian endemic Asafoetida (Ferula assa-foetida L., Apiaceae) 伊朗特有刺蒺藜离体胚种内核形态及基因组大小的变异

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1721

Narges Firoozi, G. Karimzadeh, M. S. Sabet, V. Sayadi

{"title":"Intraspecific karyomorphological and genome size variations of in vitro embryo derived Iranian endemic Asafoetida (Ferula assa-foetida L., Apiaceae)","authors":"Narges Firoozi, G. Karimzadeh, M. S. Sabet, V. Sayadi","doi":"10.36253/caryologia-1721","DOIUrl":"https://doi.org/10.36253/caryologia-1721","url":null,"abstract":"Asafoetida (Ferula assa-foetida L.) is one of the endemic medicinal plants in Iran. Analysis of karyomorphology and 2Cx DNA measurements (monoploid genome size) of 18 Iranian endemic Ferula assa-foetida populations were performed. The in vitro embryo-derived root tips were examined for karyological studies, via technique of squash and stain with 2% (w/v) aceto-orcein. Seeds of the Ferula samples and leaves of Solanum lycopersicum as standard reference (2C DNA = 1.96 pg) were stained with propidium iodide (PI), using flow cytometric (FCM) technique. All the studied populations were diploids (2n = 2x = 22) with mean chromosome length (CL) of 3.95 μm, varied from 3.05 μm (P7) to 4.94 μm (P18). The mean total chromosome volume (TCV) was 0.98 μm3, ranged from 0.47 μm3 (P7) to 1.57 μm3 (P3). Two-typed chromosomes (“m”, “sm”) formed three classes of karyotype formula. Karyotypes were mostly symmetrical and fell in 1A and 2A Stebbins category. The monoploid genome size of Iranian endemic Ferula assa-foetida populations is being stated for the first time; its mean value was 4.51 pg, ranged from 4.09 (P4) to 4.69 pg (P16). Intraspecific karyomorphological and genome size variations were clearly confirmed in studied Ferula assa-foetida.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"59 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90226530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delimiting species using DNA and morphological variation in some Alcea (Malvaceae) species based on SRAP markers 基于SRAP标记利用DNA和形态变异对某些Malvaceae种进行物种划分

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1629

Chnar Hama Noori Meerza, Basoz Sadiq Muhealdin, S. Hamarashid, Syamand Ahmad Qadir, Yusef Juan

引用次数: 0

Avicennia genus molecular phylogeny and barcoding: A multiple approach 黄花属分子系统发育与条形码:一种多方法

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1592

L. Malekmohammadi, M. Sheidai, F. Ghahremaninejad, A. Danehkar, F. Koohdar

引用次数: 0

Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry 用流式细胞术测定冬青各品种间基因组大小的差异

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1902

Peng Zhou, Jiao Li, Jing Huang, Fei Li, Qiang Zhang, M. Zhang

{"title":"Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry","authors":"Peng Zhou, Jiao Li, Jing Huang, Fei Li, Qiang Zhang, M. Zhang","doi":"10.36253/caryologia-1902","DOIUrl":"https://doi.org/10.36253/caryologia-1902","url":null,"abstract":"Ilex cornuta Lindl. & Paxton is a commercially important horticultural species worldwide, and extensive cultivation and hybridization have produced many varieties. Despite the considerable breeding, selection, widespread cultivation and domestication, which may have a significant role in the composition of genomes, there are no other previous reports of intraspecific genome size variation in the different cultivars or hybrids of this species. In the present work, genome size of 12 varieties of I. cornuta was assessed and analyzed through high-resolution flow cytometry (FCM). Nuclear DNA was analyzed using nuclei isolated from young leaves, which used propidium iodide (PI) staining, with rice (Oryza sativa cv. Nipponbare) as internal reference. As a result, statistically significant differences in genome size were detected among all diploid I. cornuta varieties considered. The estimated genome size (2C value) of I. cornuta varieties ranged from 1.47 to 1.80 pg, with 1.22-fold variation and an average size of 1.65 pg. The domestication and interspecific hybridization induced variation of genome size in I. cornuta, and the genome size of hybrids exhibited a wider range of variation compared with that of cultivars. In summary, flow cytometry is a useful tool to analyze the genome size of I. cornuta. The first report of the genome sizes of varieties of this species would provide useful data for further research on I. cornuta, and enrich the C value database of Ilex L. What’s more, our findings could be the foundation in the future of I. cornuta genome sequencing and breeding programs.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"2 1 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90805269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of chromosome and karyological analysis of Gekko nutaphandi (Gekkonidae, Squamata) from Thailand: Neo-diploid chromosome number in genus Gekko 泰国斑壁虎染色体及核学分析首次报道:斑壁虎属新二倍体染色体数目

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1875

Weera Thongnetr, S. Prasopsin, S. Aiumsumang, Sukhonthip Ditcharoen, A. Tanomtong, Prayoon Wongchantra, Wutthisak Bunnaen, S. Phimphan

{"title":"First report of chromosome and karyological analysis of Gekko nutaphandi (Gekkonidae, Squamata) from Thailand: Neo-diploid chromosome number in genus Gekko","authors":"Weera Thongnetr, S. Prasopsin, S. Aiumsumang, Sukhonthip Ditcharoen, A. Tanomtong, Prayoon Wongchantra, Wutthisak Bunnaen, S. Phimphan","doi":"10.36253/caryologia-1875","DOIUrl":"https://doi.org/10.36253/caryologia-1875","url":null,"abstract":"The karyotypes of red-eyed Gecko are not reported yet. Herein, we describe the karyotypes of red-eyed Gecko (Gekko nutaphandi Bauer, Sumontha & Pauwels, 2008) from Thailand. Gecko chromosome preparations were directly conducted from bone marrow and testis. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(GC)15 probe. The results showed that the number of diploid chromosomes is 2n=34, while the fundamental number (NF) is 46 in both males and females. The types of chromosomes were 4 large metacentric, 6 large submetacentric, 2 medium telocentric, 2 small metacentric and 20 small telocentric chromosomes. The results of conventional Giemsa staining presented the diploid chromosome number differentiation even in the same genus. NORs are located at the secondary constriction to the telomere on the long arm of chromosome pair 5. There are no sex differences in karyotypes between males and females. FISH with d(GC)15 sequences were also displayed at the telomeres of most other chromosomes. We found that during metaphase I the homologous chromosomes showed synapsis, which can be defined as 19 ring bivalents and 17 haploid chromosomes (n=17) at metaphase II as a diploid species. The karyotype formula is as follows: 2n (34) = L4m+L6sm+M2t+S2m+S20t.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"1987 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82255269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and cytogenetic characterization in experimental hybrid Aloe jucunda Reyn. x Aloe vera (L.) Burm. f. (Asphodelaceae) 实验杂交芦荟形态及细胞遗传学特征。x芦荟(L.)发热管。f。(Asphodelaceae)

IF 2.1 4区生物学

Caryologia Pub Date : 2023-04-28 DOI: 10.36253/caryologia-1886

Wendy Ozols-Narbona, José Imery-Buiza

{"title":"Morphological and cytogenetic characterization in experimental hybrid Aloe jucunda Reyn. x Aloe vera (L.) Burm. f. (Asphodelaceae)","authors":"Wendy Ozols-Narbona, José Imery-Buiza","doi":"10.36253/caryologia-1886","DOIUrl":"https://doi.org/10.36253/caryologia-1886","url":null,"abstract":"Aloe L. includes plants of economic interest worldwide for their medicinal properties and ornamental character. In this study, morphological and cytogenetic traits were evaluated in a hybrid obtained using Aloe jucunda Reyn. as pollen donor and A. vera (L.) Burm. f. as female parent, to characterize it, determine its ornamental and agronomic potentialities and aspects related to its reproduction. Conventional protocols for morphometric studies and cytogenetic analysis described for succulent plants were applied. Progeny showed intermediate expressiveness in most of the characteristics, except in the colour of the leaves and flowers (hybrid = A. jucunda), as well as for the length of teeth, number, and area of leaf spots and angle between continuous leaves, where it surpassed the expression of both parents, giving it a high ornamental value. The length, width, and thickness of the leaves improved with respect to the paternal genome, so its potential for the exploitation of the gel and latex of its leaves cannot be ruled out. Root tip cells showed a karyotype 2n = 2x = 14 = 8L + 6S = 1L(smsat) + 1L(sm) + 3L(st) + 3L(smsat) + 1S(m) + 5S(sm). Microsporogenesis showed chromosomal abnormalities in 47.4% of the meiocytes, the most frequent being micronuclei in prophase-I, sticky chromosomes in metaphase-I, one or two dicentric bridges accompanied or not by acentric fragments in anaphase-I, -II, and telophase-I, - II, as well as one or two additional microspores. These abnormalities reduce the fertility of their pollen grains and limit their sexual reproduction, providing a better explanation for their sterility.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":"46 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84127969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0