蒺藜对洋葱和蚕豆分生组织细胞的细胞遗传学影响。

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2023-09-19 DOI:10.36253/caryologia-2092

Ali Bouzekri, Meryem Nassar, Souheila Slimani, Zohra Chekroud

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引用次数: 0

摘要

蒺藜是蒺藜科的一种植物，由于其药理成分的治疗作用，在世界范围内经常被用于治疗各种疾病。本研究利用洋葱和蚕豆两种植物模型，研究了土霉的细胞毒性和基因毒性作用。分别以0.00625、0.0125、0.025、0.05、0.1mg/mL的提取物对蚕豆根分生组织细胞进行作用试验。这种评估包括对根生长、结构和颜色的研究，以及有丝分裂指数(MI)和染色体畸变(CAs)作为毒性的准确指标的测定。结果表明，在0.025、0.05和0.1 mg/ml的浓度和0.1 mg/ml的浓度下，紫花苜蓿和蚕豆的平均根长均显著降低。细胞毒性和遗传毒性结果表明，紫花苜蓿和蚕豆的心肌梗死浓度分别从0.025 mg/ml和0.05 mg/ml显著降低，心肌梗死的降低与紫花苜蓿浓度和处理时间的增加有关。此外，在0.025 mg/ml浓度下，a . cepa和V. faba中CAs含量显著增加。MI和CAs丰度的显著降低表明土霉具有遗传毒性。因此，地草是一种药用植物，应谨慎、适当地使用，并根据基本的治疗需要。

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Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.

Tribulus terrestris is a plant of the Zygophyllaceae family frequently used worldwide to treat various diseases due to the therapeutic effects of its pharmacological components. This study examines the cytotoxic and genotoxic effects of T. terrestris using two plant models, Allium cepa and Vicia faba. Extracts of 0.00625, 0.0125, 0.025, 0.05 and 0.1mg/mL were tested on meristematic cells of A. cepa and V. faba roots. This assessment includes the study of root growth, structure and coloration, as well as the determination of the mitotic index (MI) and chromosomal aberrations (CAs) as accurate indicators of toxicity. Our results showed a significant decrease in the mean length of roots treated with 0.025, 0.05 and 0.1 mg/ml for A. cepa and 0.1 mg/ml for V. faba. Cytotoxicity and genotoxicity results showed a significant decrease in MI from 0.025 mg/ml in A. cepa and from 0.05 mg/ml in V. faba, and this decrease in MI is linked to the increase in concentration and treatment time with T. terrestris. Furthermore, a significant increase in CAs was observed in A. cepa and V. faba from the 0.025 mg/ml concentration. The significant reduction in MI and CAs abundance suggests the genotoxicity of T. terrestris. Therefore, T. terrestris is a medicinal plant that should be used with caution, appropriately and based on essential therapeutic needs.

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.