伊朗特有刺蒺藜离体胚种内核形态及基因组大小的变异

IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences
Narges Firoozi, G. Karimzadeh, M. S. Sabet, V. Sayadi
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引用次数: 0

摘要

阿魏(Ferula assa-foetida L.)是伊朗特有药用植物之一。对18个伊朗阿魏(Ferula assa-foetida)地方性群体进行了核形态分析和2Cx DNA测定(单倍体基因组大小)。采用2% (w/v)乙酰乙醇酸(aceto-orcein)压扁染色技术,对离体胚源根尖进行核学研究。采用流式细胞术(FCM)对阿魏样品种子和番茄茄叶片(2C DNA = 1.96 pg)进行碘化丙啶(PI)染色。所有群体均为二倍体(2n = 2x = 22),平均染色体长度(CL)为3.95 μm,变化范围为3.05 ~ 4.94 μm (P18)。平均染色体总体积(TCV)为0.98 μm3,范围为0.47 μm3 (P7) ~ 1.57 μm3 (P3)。两型染色体(“m”、“sm”)形成了三类核型公式。核型多为对称型,属于1A和2A Stebbins范畴。首次报道了伊朗阿魏特有种群的单倍体基因组大小;平均值为4.51 pg,范围为4.09 (P4) ~ 4.69 pg (P16)。在研究的阿魏中,种内核形态和基因组大小的差异得到了明确的证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intraspecific karyomorphological and genome size variations of in vitro embryo derived Iranian endemic Asafoetida (Ferula assa-foetida L., Apiaceae)
Asafoetida (Ferula assa-foetida L.) is one of the endemic medicinal plants in Iran. Analysis of karyomorphology and 2Cx DNA measurements (monoploid genome size) of 18 Iranian endemic Ferula assa-foetida populations were performed. The in vitro embryo-derived root tips were examined for karyological studies, via technique of squash and stain with 2% (w/v) aceto-orcein. Seeds of the Ferula samples and leaves of Solanum lycopersicum as standard reference (2C DNA = 1.96 pg) were stained with propidium iodide (PI), using flow cytometric (FCM) technique. All the studied populations were diploids (2n = 2x = 22) with mean chromosome length (CL) of 3.95 μm, varied from 3.05 μm (P7) to 4.94 μm (P18). The mean total chromosome volume (TCV) was 0.98 μm3, ranged from 0.47 μm3 (P7) to 1.57 μm3 (P3). Two-typed chromosomes (“m”, “sm”) formed three classes of karyotype formula. Karyotypes were mostly symmetrical and fell in 1A and 2A Stebbins category. The monoploid genome size of Iranian endemic Ferula assa-foetida populations is being stated for the first time; its mean value was 4.51 pg, ranged from 4.09 (P4) to 4.69 pg (P16). Intraspecific karyomorphological and genome size variations were clearly confirmed in studied Ferula assa-foetida.
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来源期刊
Caryologia
Caryologia 生物-遗传学
CiteScore
1.60
自引率
23.80%
发文量
26
审稿时长
12 months
期刊介绍: Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.
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