Phenthoate toxicity evaluation in root meristem of Pisum sativum L.

IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences
Sazada Siddiqui
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引用次数: 0

Abstract

Phenthoate is an organothiophosphate insecticide. Effect of phenthoate on the cytogenetic alterations in root tip cells of Pisum sativum L., a multiuse crop was investigated in this study. Pisum sativum L. seeds were exposed to different concentrations of phenthoate (0.1, 0.2, 0.3, 0.4, and 0.5%) and were germinated at 24°C for 72 hours and cytogenetic alterations were assessed. Analysis of mitotic index revealed that phenthoate has cytotoxic attributes, and cell proliferation kinetics frequencies showed alterations in the kinetics of the mitotic process. Phenthoate treatment of 0.1% to 0.5% resulted in an increase in the metaphases, and a reduction in prophases, anaphases, and telophases ratio, dose dependently. The findings of the study reveal that, phenthoate reduced the percentage of seed germination, mitotic index, radicle length and increased chromosomal abnormalities dose dependently. Root tip cells of Pisum sativum L. seeds treated with phenthoate showed an increased occurrence of single and double bridges, fragments, stickiness, laggard, and vagrants.
Phenthoate对Pisum sativum L根分生组织的毒性评价。
Phenthoate是一种有机硫代磷酸盐杀虫剂。以多用途作物Pisum sativum L.为研究对象,研究了施用苯甲酸酯对其根尖细胞遗传变化的影响。将Pisum sativum L.种子暴露于不同浓度(0.1,0.2,0.3,0.4和0.5%)的苯多酸盐中,在24°C下萌发72小时,并评估细胞遗传学变化。有丝分裂指数分析表明,苯甲酸酯具有细胞毒性,细胞增殖动力学频率显示有丝分裂过程动力学的改变。0.1% ~ 0.5%苯thoate处理导致中期增加,前期、后期和末期比例减少,剂量依赖。研究结果表明,苯甲酸酯降低了种子发芽率、有丝分裂指数、胚根长度和增加染色体异常呈剂量依赖性。phenthoate处理后的Pisum sativum L.种子根尖细胞单桥和双桥、碎片、粘滞、滞后和游子的发生率增加。
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来源期刊
Caryologia
Caryologia 生物-遗传学
CiteScore
1.60
自引率
23.80%
发文量
26
审稿时长
12 months
期刊介绍: Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.
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