Cytotoxic and Genotoxic Effects of Methanol Extracts of Some Gypsophila L. Species

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2021-08-09 DOI:10.36253/caryologia-1091

Ferhan Korkmaz, Özgün Tuna Gülören, Meltem Erdi̇r, E. Ataşlar

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Abstract

In this study, the cytotoxic and genotoxic effects of Gypsophila perfoliata L. var. perfoliata, Gypsophila perfoliata L. var. araratica Kit Tan, Gypsophila pilosa Hudson and Gypsophila osmangaziensis Ataşlar & Ocak plant extracts have been examined by using Allium Test method. Methanol extracts of plants have been prepared in 4 different concentrations (0.625 mg/ml, 1.250 mg/ml, 2.500 mg/ml and 5.000 mg/ml). After the onion roots were treated at these concentrations of plant extracts for 24 hours and 48 hours, mitosis preparations were prepared from these root tips. With the data being obtained by evaluating the preparations, mitotic index (%) and chromosome aberration (%) values have been calculated. Distilled water has been used as control group. It was found out that mitotic index and chromosome aberration values of all species showed significant differences compared to control group in the extract concentration range of 1.250–5.000 mg/ml. It has been determined that the most widely observed chromosome aberrations were disturbed metaphase, sticky metaphase, c-metaphase, disturbed anaphase and anaphase bridge.

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部分Gypsophila L.种甲醇提取物的细胞毒性和基因毒性作用

本研究采用葱试验法研究了细叶Gypsophila perfoliata L. var. perfoliata、细叶Gypsophila perfoliata L. var. araratica Kit Tan、细叶Gypsophila pilosa Hudson和细叶Gypsophila osmangaziensis ata & Ocak植物提取物的细胞毒和基因毒作用。以4种不同浓度(0.625 mg/ml、1.250 mg/ml、2.500 mg/ml和5.000 mg/ml)制备了植物甲醇提取物。在这些浓度的植物提取物处理洋葱根24小时和48小时后，从这些根尖制备有丝分裂制剂。根据评价所得数据，计算有丝分裂指数(%)和染色体畸变(%)值。以蒸馏水为对照组。结果表明，在提取液浓度为1.250 ~ 5.000 mg/ml范围内，各物种的有丝分裂指数和染色体畸变值均与对照组有显著差异。已确定最广泛观察到的染色体畸变是干扰中期、粘滞中期、c-中期、干扰后期和后期桥。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.