{"title":"利用序列相关扩增多态性分析牛虻(Boraginaceae-Cynoglosseae)的形态计量学和遗传多样性","authors":"X. Yao, Haodong Liu, M. S. Tabarestani","doi":"10.36253/caryologia-1380","DOIUrl":null,"url":null,"abstract":"The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.","PeriodicalId":9634,"journal":{"name":"Caryologia","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Morphometric analysis and genetic diversity in Rindera (Boraginaceae-Cynoglosseae) using sequence related amplified polymorphism\",\"authors\":\"X. Yao, Haodong Liu, M. S. Tabarestani\",\"doi\":\"10.36253/caryologia-1380\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.\",\"PeriodicalId\":9634,\"journal\":{\"name\":\"Caryologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2022-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Caryologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.36253/caryologia-1380\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Caryologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.36253/caryologia-1380","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Morphometric analysis and genetic diversity in Rindera (Boraginaceae-Cynoglosseae) using sequence related amplified polymorphism
The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.
期刊介绍:
Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.