Morphometric analysis and genetic diversity in Rindera (Boraginaceae-Cynoglosseae) using sequence related amplified polymorphism

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2022-03-08 DOI:10.36253/caryologia-1380

X. Yao, Haodong Liu, M. S. Tabarestani

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引用次数: 0

Abstract

The genus Rindera comprises about 20–25 species distributed in central eastern Europe to central Asia. Ninety-five individuals related to six Rindera were collected in 9 provinces. A total of 147 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of six Rindera species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 8 to 22. ). The predicted unbiased heterozygosity (H) varied between 0.15 (Rindera media) and 0.30 (Rindera regia). High Shannon’s information index was detected in Rindera regia. The genetic similarities between six species are estimated from 0.73 to 0.95. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Rindera regia and Rindera media had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Rindera species. Current results have implications in biodiversity and conservation programs.

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利用序列相关扩增多态性分析牛虻(Boraginaceae-Cynoglosseae)的形态计量学和遗传多样性

Rindera属包括约20-25种，分布在中欧东欧到中亚。在9个省收集到与6种林德拉有关的95个个体。通过聚合酶链反应(PCR)对6种牛头虫进行扩增，共扩增出147条(NTL) DNA条带。这些条带由10个选择性引物组合而成。扩增片段总数为8 ~ 22个。)．预测的无偏杂合度(H)在0.15 (rrindera media)和0.30 (rrindera regia)之间变化。在林德拉地区检测到较高的香农信息指数。6种间的遗传相似度在0.73 ~ 0.95之间。聚类结果显示两个主要聚类。SRAP (Sequence-related amplified polymorphism，序列相关扩增多态性)标记分析表明，帝王牛虻与媒介牛虻的相似性最低。本研究还发现了距离隔离的显著特征(Mantel测试结果)。结果表明，序列相关的扩增多态性具有鉴定和破译林蝇属亲缘关系的潜力。目前的结果对生物多样性和保护计划具有启示意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.