Cytogenetic and cytological analysis of advanced genetic material of cape gooseberry Physalis peruviana for use in plant breeding

IF 2.1 4区 生物学 Q2 Agricultural and Biological Sciences
Clara Viviana FRANCO FLOREZ, Sara Alejandra LIBERATO GUIO, Erika Patricia SANCHEZ BETANCOURT, Francy Liliana GARCÍA ARIAS, V. M. Núñez Zarantes
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Abstract

The cape gooseberry, Physalis peruviana L., is a crop that is transitioning from a semi-wild rural food source to becoming an international export commodity fruit deserving of greater attention from the scientific community, producers, policy makers and opinion makers. Despite its importance, the crop has serious technological development challenges, mainly associated with the limited supply of genetically improved materials for producers and consumers. In the present study, the level of ploidy of 100 genotypes of gooseberry from a working collection was determined by counting the number of chromosomes and chloroplasts, to include them in the breeding program. The number of chromosomes in dividing cells of root-tip meristems, as well as the number of chloroplasts per guard cell, from plants grown in vitro and ex vitro conditions were determined. Haploid with 24 chromosomes, doubled haploid-tetraploid with 48 chromosomes, aneuploid (44 and 49 chromosomes) and mixoploid genotypes with 36 to 86 chromosomes were found. The number of chloroplasts / cell guard ranged from 4-8, 6-16, 7-16 and 9-21 for the haploid, aneuploid, doubled haploid-tetraploid and mixoploid genotypes, respectively. Evidence of a high cytogenetic diversity in the evaluated genotypes.
猕猴桃高级遗传物质在植物育种中的细胞遗传学和细胞学分析
南非醋栗(Physalis peruviana L.)是一种正在从半野生的农村食物来源转变为国际出口商品水果的作物,值得科学界、生产者、决策者和舆论制定者给予更多关注。尽管这种作物很重要,但它在技术发展方面面临着严峻的挑战,主要与生产者和消费者获得的基因改良材料供应有限有关。在本研究中,通过对工作采收的100个基因型鹅莓的染色体和叶绿体数量的计数,确定了其倍性水平,并将其纳入育种计划。测定了在离体和离体条件下生长的植物根尖分生组织分裂细胞的染色体数和每个保卫细胞的叶绿体数。单倍体有24条染色体,双单倍体-四倍体有48条染色体,非整倍体有44条和49条染色体,混倍体有36 ~ 86条染色体。单倍体、非整倍体、双倍单倍体-四倍体和混合倍体基因型的叶绿体/细胞保卫数分别为4-8、6-16、7-16和9-21。在评估的基因型中具有高细胞遗传多样性的证据。
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来源期刊
Caryologia
Caryologia 生物-遗传学
CiteScore
1.60
自引率
23.80%
发文量
26
审稿时长
12 months
期刊介绍: Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.
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