Molecular identification and genetic relationships among Alcea (Malvaceae) species by ISSR Markers: A high value medicinal plant

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2021-12-21 DOI:10.36253/caryologia-1330

Jinxin Cheng, Dingyu Hu, Yaran Liu, Zetian Zhang, M. Khayatnezhad

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Abstract

Alcea L. is one of the largest genera of Malvaceae family with nearly 70 species worldwide mainly distributed in SW Asia. According to the latest revision of the family, it is represented by 34 species in the Flora of Iran, among them, 15 species are endemic. It is tough to accurate germplasm/ plant recognition by using morphological characteristics because of its propagation, growing and using. We conducted a molecular data analysis on these plant species due to their importance. We examined 156 plants from 14 species in 16 regions that were selected randomly for this investigation. It has been 119 polymorphic bands (94.33%) were resulted from 128 bands of 10 primers in amplification of genomic DNA. ISSR primers have a great capacity to detect polymorphic loci among Alcea species, as evidenced by the high average PIC and MI values found. The genetic similarity of 14 species was calculated and ranged between 0.635 to 0.990. Inter-Simple sequence repeats (ISSR) markers research revealed that Alcea tarica Pakravan & Ghahreman and Alcea kopetdaghensis lljin had the least similarity, while Alcea semnanica Pakravan and Alcea mazandaranica Pakravan & Ghahreman had the most. The current study attempts to answer three questions: 1) can ISSR markers identify Alcea species? 2) what is the genetic structure of these taxa in Iran? and 3) what is the inter-relationship between these taxa? The current study discovered that ISSR markers can be used to identify species.

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高价值药用植物锦葵属植物的ISSR分子鉴定及其亲缘关系

Alcea L.是锦葵科最大的属之一，在世界上有近70种，主要分布在东南亚。根据该科的最新修订，在伊朗植物区系中有34种，其中特有种15种。由于其繁殖、生长和利用等方面的原因，利用形态特征进行准确的种质/植物识别较为困难。鉴于这些植物的重要性，我们对它们进行了分子数据分析。随机选取16个地区14种156株植物进行调查。在10条引物的128条条带中扩增出119条多态性条带(94.33%)。ISSR引物具有很强的多态性位点检测能力，发现的平均PIC和MI值较高。计算了14种的遗传相似度，遗传相似度在0.635 ~ 0.990之间。ISSR标记研究表明，Alcea tarica Pakravan & Ghahreman和Alcea kopetdaghensis lljin的相似性最低，Alcea semnanica Pakravan和Alcea mazandaranica Pakravan & Ghahreman的相似性最高。目前的研究试图回答三个问题:1)ISSR标记能否识别藻属?2)伊朗这些分类群的遗传结构如何?3)这些分类群之间的相互关系是什么?目前的研究发现，ISSR标记可以用来识别物种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.