The Kaohsiung journal of medical sciences最新文献_第3页

Hypoxia Triggers ALYREF-Mediated m⁵C Methylation of KIF20A to Activate KIF20A/BUB1 for Generating Ferroptosis Resistance in Cervical Cancer Cells. 缺氧触发alyref介导的KIF20A m5C甲基化，激活KIF20A/BUB1，在宫颈癌细胞中产生铁下沉抗性。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-07 DOI: 10.1002/kjm2.70093

Yan Gao, Ting Zou

{"title":"Hypoxia Triggers ALYREF-Mediated m5C Methylation of KIF20A to Activate KIF20A/BUB1 for Generating Ferroptosis Resistance in Cervical Cancer Cells.","authors":"Yan Gao, Ting Zou","doi":"10.1002/kjm2.70093","DOIUrl":"https://doi.org/10.1002/kjm2.70093","url":null,"abstract":"Ferroptosis resistance is a key player in cervical cancer (CC) development. Hypoxia is a negative factor affecting CC treatment by inducing ferroptosis resistance. Our study aimed to investigate the detailed mechanisms of hypoxia-induced ferroptosis resistance in CC cells. The mRNA and protein levels were assessed using RT-qPCR and western blot. Immunofluorescence staining was used to analyze the co-localization of Budding uninhibited by benzimidazole 1 (BUB1) and Kinesin family member 20A (KIF20A) in CC cells. Fe2+, MDA, and GSH levels were measured by commercial kits. Cell viability was determined by CCK-8. The molecular interactions were analyzed by RIP or Co-IP. MeRIP was adopted to measure the m5C level of KIF20A. We found that hypoxia facilitated ferroptosis resistance in CC cells. Hypoxia led to the upregulation of KIF20A, and KIF20A knockdown weakened hypoxia-mediated ferroptosis resistance in CC cells. Mechanistically, Aly/REF export factor (ALYREF) stabilized KIF20A mRNA stability via m5C modification. In addition, KIF20A upregulated BUB1 in CC cells by directly interacting with BUB1. Rescue experiments indicated that BUB1 overexpression partially reversed the inhibitory effect of KIF20A knockdown on hypoxia-mediated ferroptosis resistance in CC cells. In conclusion, hypoxia triggers ALYREF-mediated m5C methylation of KIF20A to activate the KIF20A/BUB1 axis, thereby inducing ferroptosis resistance in CC cells.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70093"},"PeriodicalIF":3.1,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145017041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exercise Capacity and Pulmonary Function in Pediatric Patients With Anomalous Pulmonary Venous Connection Post-Surgical Repair: A Retrospective Analysis. 术后肺静脉连接异常患儿的运动能力和肺功能：回顾性分析。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-03 DOI: 10.1002/kjm2.70101

Yen-Hsien Wu, Yen-Sen Lu, Sheng-Hui Tuan, Yi-Ching Liu, I-Ching Huang, Yi-Cheng Wang, Tang-Hsu Hsieh, Shih-Hsing Lo, Ko-Long Lin, Jong-Hau Hsu

{"title":"Exercise Capacity and Pulmonary Function in Pediatric Patients With Anomalous Pulmonary Venous Connection Post-Surgical Repair: A Retrospective Analysis.","authors":"Yen-Hsien Wu, Yen-Sen Lu, Sheng-Hui Tuan, Yi-Ching Liu, I-Ching Huang, Yi-Cheng Wang, Tang-Hsu Hsieh, Shih-Hsing Lo, Ko-Long Lin, Jong-Hau Hsu","doi":"10.1002/kjm2.70101","DOIUrl":"10.1002/kjm2.70101","url":null,"abstract":"Anomalous pulmonary venous connection (APVC), including total (TAPVC) and partial (PAPVC) forms, is a congenital heart defect with abnormal pulmonary vein drainage; and while surgical repair has improved survival, its long-term impact on cardiopulmonary function remains unclear. This retrospective study evaluated exercise capacity and pulmonary function in 26 pediatric APVC patients (17 TAPVC, 9 PAPVC) using cardiopulmonary exercise testing (CPET) and compared them with 63 age-matched healthy controls. Patients with complex defects or significant comorbidities were excluded. Results showed significantly lower anaerobic threshold VO2 (p = 0.03), peak VO2 (p < 0.001) and peak heart rate (p = 0.02) in the APVC group, indicating impaired exercise capacity; though no differences were found between TAPVC and PAPVC subgroups. Despite preserved resting lung function, these findings suggest that children with repaired APVC experience persistent exercise limitations, underscoring the importance of routine functional assessment and potential rehabilitation, with further studies needed to clarify underlying mechanisms and guide long-term care.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70101"},"PeriodicalIF":3.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deletion of NFIA Leads to Activation of S100A7 and Inflammatory Response-Induced Apoptosis of Keratinocytes in Oral Lichen Planus Progression. NFIA缺失导致口腔扁平苔藓进展中S100A7激活和炎症反应诱导的角化细胞凋亡

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 Epub Date: 2025-06-25 DOI: 10.1002/kjm2.70052

Maimaiti Tudi, Zumulaiti Aierken, Maihebubaimu Tuerxun, Maimaitituxun Tuerdi

{"title":"Deletion of NFIA Leads to Activation of S100A7 and Inflammatory Response-Induced Apoptosis of Keratinocytes in Oral Lichen Planus Progression.","authors":"Maimaiti Tudi, Zumulaiti Aierken, Maihebubaimu Tuerxun, Maimaitituxun Tuerdi","doi":"10.1002/kjm2.70052","DOIUrl":"10.1002/kjm2.70052","url":null,"abstract":"S100 calcium-binding protein A7 (S100A7) has been implicated in psoriasis and other inflammatory diseases. However, the function of S100A7 in oral lichen planus (OLP), a chronic inflammatory disease, remains unclear. OLP was induced in mice by transplanting human OLP lesions into the backs of thymus-free mice, and an in vitro cell model was constructed using LPS-stimulated HaCaT cells. Gene intervention was performed using shRNA lentiviral vectors. The secretion of the pro-inflammatory factors IL-6 and TNF-α, as well as the rate of apoptosis in HaCaT cells, was also assessed. ChIP assay and dual-luciferase assay were used to validate the transcriptional regulation of S100A7 by nuclear factor 1 A-type (NFIA). The expression of S100A7 was significantly elevated in the lesion tissues of OLP-induced mice. Knockdown of S100A7 alleviated inflammation and reduced keratinocyte apoptosis. The transcription factor NFIA repressed S100A7 expression by binding to the S100A7 promoter. The overexpression of NFIA ameliorated inflammation in vivo and reduced apoptosis in vitro, which was abrogated by further overexpression of S100A7. Overall, our results indicate that NFIA reduces inflammatory response-induced keratinocyte apoptosis in OLP by inhibiting S100A7 transcription.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70052"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144500001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia-Enhanced Wharton's Jelly Mesenchymal Stem Cell Therapy for Liver Fibrosis: A Comparative Study in a Rat Model. 缺氧增强的沃顿果冻间充质干细胞治疗肝纤维化：大鼠模型的比较研究。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 Epub Date: 2025-06-30 DOI: 10.1002/kjm2.70053

Wei-Ting Kuo, Chen-Yuan Hsiao, Sheng-Hao Chiu, Shu-Cheng Chou, Ching-Shu Chiang, Jui-Yu Chen, Solomon Chih-Cheng Chen, Tien-Hua Chen, Jia-Fwu Shyu, Chi-Hung Lin, Pei-Jiun Tsai

{"title":"Hypoxia-Enhanced Wharton's Jelly Mesenchymal Stem Cell Therapy for Liver Fibrosis: A Comparative Study in a Rat Model.","authors":"Wei-Ting Kuo, Chen-Yuan Hsiao, Sheng-Hao Chiu, Shu-Cheng Chou, Ching-Shu Chiang, Jui-Yu Chen, Solomon Chih-Cheng Chen, Tien-Hua Chen, Jia-Fwu Shyu, Chi-Hung Lin, Pei-Jiun Tsai","doi":"10.1002/kjm2.70053","DOIUrl":"10.1002/kjm2.70053","url":null,"abstract":"Liver fibrosis is a progressive disease that can lead to cirrhosis and liver failure, with limited treatment options. Wharton's jelly-derived MSCs (WJ-MSCs) have immunomodulatory and antifibrotic potential. Hypoxia preconditioning enhances MSC survival and paracrine activity, but its effects in liver fibrosis remain unclear. This study compares hypoxia and normoxia WJ-MSCs in a CCl4-induced liver fibrosis rat model. Sprague-Dawley rats received chronic CCl4 to induce fibrosis. At Week 8, normoxia or hypoxia WJ-MSCs were injected via the portal vein. Liver function was assessed using biochemical markers (ALT, AST, T-Bil, albumin), PET/MR imaging, and qPCR for IL-1β and IL-6. Fibrosis regression was evaluated via ultrasound, histology, and collagen quantification. Regeneration was analyzed through Ki67 immunostaining and qPCR for Ki67 and HGF. MSC engraftment was determined by hNA immunohistochemistry. Both normoxia and hypoxia WJ-MSCs improved liver function, with hypoxia WJ-MSCs showing greater AST and T-Bil reductions. PET/MR imaging demonstrated superior metabolic recovery in the hypoxia group, with greater 18F-FDG uptake reduction. Histological analysis confirmed more significant fibrosis regression and collagen reduction in the hypoxia group. Gene expression analysis showed stronger suppression of TGF-β, α-SMA, and collagen I. Liver regeneration markers Ki67 and HGF were significantly upregulated with a greater HGF increase in the hypoxia group. Additionally, hypoxia WJ-MSCs exhibited higher engraftment and reduced pulmonary entrapment, indicating improved liver homing. Both normoxia and hypoxia WJ-MSCs improved liver fibrosis, but hypoxia preconditioning further enhanced liver function, fibrosis regression, and metabolic recovery, supporting its therapeutic superiority.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70053"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association Between Angiotensin-Converting Enzyme (ACE) Gene Insertion/Deletion (I/D) Polymorphism Genotypes With Brain Volume and Hypertension in Alzheimer's Disease-A Retrospective Study. 血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性基因型与阿尔茨海默病脑容量和高血压的关系——一项回顾性研究

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 Epub Date: 2025-05-15 DOI: 10.1002/kjm2.70046

Bin-Tse Lin, Ching-Fang Chien, Ling-Chun Huang, Yuan-Han Yang

{"title":"Association Between Angiotensin-Converting Enzyme (ACE) Gene Insertion/Deletion (I/D) Polymorphism Genotypes With Brain Volume and Hypertension in Alzheimer's Disease-A Retrospective Study.","authors":"Bin-Tse Lin, Ching-Fang Chien, Ling-Chun Huang, Yuan-Han Yang","doi":"10.1002/kjm2.70046","DOIUrl":"10.1002/kjm2.70046","url":null,"abstract":"This study investigates the role of the ACE I/D polymorphism in Alzheimer's disease (AD) patients, particularly in relation to hypertension and its influence on brain volume. Seventy-seven AD patients, diagnosed based on Aging and Alzheimer's Association criteria, were enrolled from the Kaohsiung Municipal Ta-Tung Hospital Dementia Cohort. ACE I/D genotypes were identified through polymerase chain reaction, and various factors such as age, sex, education, brain volume, and neuropsychological test scores were analyzed. The results indicated that ACE genotypes, presence of apolipoprotein epsilon 4 (APOEε4), and brain volume did not significantly differ between patients with and without hypertension. While age and sex were associated with gray matter volume, cerebrospinal fluid volume correlated with age, sex, and hypertension. Total cranial volume was linked to sex, and the cerebrospinal fluid-to-total intracranial volume ratio was influenced by sex and education. Overall, ACE I/D genotypes and APOEε4 did not have a significant impact on brain volume in AD patients, regardless of hypertension status. Instead, brain atrophy was associated with sex, age, education, and hypertension. These findings suggest that although ACE may not significantly influence brain volume in AD patients, further large-scale studies are needed to clarify its role in AD pathogenesis.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70046"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large Cell Neuroendocrine Carcinoma of the Cervix With Extensive Metastases. 宫颈大细胞神经内分泌癌伴广泛转移。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 DOI: 10.1002/kjm2.70100

Di-Ping Yu, Li-Mei Sun

引用次数: 0

Relaxin-2 Ameliorates Spinal Cord Injury by Inhibiting Microglia Activation. 松弛素-2通过抑制小胶质细胞激活改善脊髓损伤。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 Epub Date: 2025-05-20 DOI: 10.1002/kjm2.70041

Ji-Huan Wang, Hong-Biao Sheng, Jun-Kun Li

{"title":"Relaxin-2 Ameliorates Spinal Cord Injury by Inhibiting Microglia Activation.","authors":"Ji-Huan Wang, Hong-Biao Sheng, Jun-Kun Li","doi":"10.1002/kjm2.70041","DOIUrl":"10.1002/kjm2.70041","url":null,"abstract":"This study aims to assess the therapeutic effectiveness of Relaxin-2 (RLN-2) in promoting functional recovery and neuroprotection following spinal cord injury (SCI) in mice. Furthermore, continuous subcutaneous infusion of Serelaxin (0.5 mg/kg/day; human recombinant relaxin-2) improved neurological recovery, as evidenced by higher Basso-Beattie-Bresnahan (BBB) scores and reduced foot-stepping angles compared to the SCI group. Additionally, RLN-2 effectively reduced edema in the injured spinal cord, as shown by decreased water content and downregulated AQP4 expression at mRNA and protein levels. RLN-2 reduced oxidative stress markers such as malondialdehyde (MDA) and reactive oxygen species (ROS) and increased the activity of catalase (CAT). Further, RLN-2 mitigated neuroinflammation by reducing the levels of pro-inflammatory cytokines (TNF-α and IL-6) and by inhibiting the activation of M1 microglia while promoting the polarization of M2 microglia. It also inhibited the activation of the NF-κB signaling and strengthened the activation of the STAT6 signaling in the spinal cord of SCI mice. These findings suggest that RLN-2 may be a promising therapeutic agent for the treatment of spinal cord injury.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70041"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Add-On Hepatobiliary Abbreviated Magnetic Resonance Imaging on Ultrasound Hepatoma Surveillance for Liver Cirrhosis- a Randomized Study. 一项随机研究：附加肝胆缩短磁共振成像对肝硬化超声肝癌监测的影响。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 DOI: 10.1002/kjm2.70104

Jing-Houng Wang, Hsin-You Ou, Yi-Hao Yen, Chao-Hung Hung, Sheng-Nan Lu

{"title":"Impact of Add-On Hepatobiliary Abbreviated Magnetic Resonance Imaging on Ultrasound Hepatoma Surveillance for Liver Cirrhosis- a Randomized Study.","authors":"Jing-Houng Wang, Hsin-You Ou, Yi-Hao Yen, Chao-Hung Hung, Sheng-Nan Lu","doi":"10.1002/kjm2.70104","DOIUrl":"https://doi.org/10.1002/kjm2.70104","url":null,"abstract":"Hepatocellular carcinoma (HCC) surveillance with semi-annual ultrasound (US) is recommended for high-risk patients. This study investigates the impact of hepatobiliary abbreviated magnetic resonance imaging (AMRI) performed annually on the recommended US surveillance. Patients with compensated liver cirrhosis at regular HCC surveillance using US and alpha-fetoprotein, with adequate renal function and without HCC diagnosis, were enrolled. Patients were randomized into add-on hepatobiliary AMRI and continuous US surveillance groups. For patients in the AMRI group, gadoxetic acid-enhanced AMRI was performed at enrollment and annually. Liver nodule detection, HCC diagnostic tests, and HCC development were compared between the two groups. One hundred and four patients were initially enrolled, with 15 patients excluded for loss of regular follow-up, giving a total of 89 patients (AMRI: 45 and US: 44) that were analyzed in a median follow-up of 33.6 months. There were no significant differences in baseline characteristics nor statistical differences in hepatic nodule detections (AMRI:10 vs. US:18, p = 0.074) and HCC developments (1 vs. 6, p = 0.058) between the groups. While one HCC with a size of 1.2 cm (BCLC stage:0) was diagnosed in the AMRI group, six HCCs with a mean size of 2.4 cm (BCLC stage 0:2, A:3, B:1) were found in the US group. Compared with the AMRI group, there were more patients in the US group (18 vs. 9, p = 0.032) underwent dynamic imaging and/or biopsy. Curative treatments were performed for all patients with HCC. For compensated cirrhosis patients in the recommended US surveillance, hepatobiliary AMRI annually might reduce the frequency of HCC diagnostic tests.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70104"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYB Activates the Hedgehog Signaling Pathway to Repress Natural Killer Cytotoxicity in Cervical Cancer. MYB激活Hedgehog信号通路抑制宫颈癌自然杀伤细胞毒性

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 DOI: 10.1002/kjm2.70084

Yu Wang, Chen Li

{"title":"MYB Activates the Hedgehog Signaling Pathway to Repress Natural Killer Cytotoxicity in Cervical Cancer.","authors":"Yu Wang, Chen Li","doi":"10.1002/kjm2.70084","DOIUrl":"https://doi.org/10.1002/kjm2.70084","url":null,"abstract":"Natural killer (NK) cells present in the tumor microenvironment serve as a critical line of defense against various malignancies, including cervical cancer. While MYB is known to drive malignancy progression, its influence on NK cell activity remains poorly understood. This study aimed to elucidate the role of MYB in regulating NK cell cytotoxicity and its underlying mechanism in cervical cancer cells. MYB expression in cervical cancer tissues and cells was analyzed using bioinformatics and qRT-PCR. Cell viability was assessed via CCK-8 assay, while NK cell-mediated killing of cervical cancer cells was evaluated through cytotoxicity assays. The expression levels of cytotoxic factors (IFN-γ and TNF-α) were measured by ELISA, whereas perforin and granzyme B were detected via immunofluorescence. Apoptosis was analyzed using flow cytometry. To investigate the impact of MYB on the hedgehog signaling pathway, the expression levels of related factors (PTCH1, Gli1, and Gli2) were assessed using qRT-PCR and Western blot. Bioinformatics and qRT-PCR analyses revealed MYB overexpression in cervical cancer. Signaling pathway prediction indicated MYB enrichment in cytotoxic signaling pathways. Functional experiments demonstrated that MYB overexpression activated the hedgehog signaling pathway, thereby suppressing NK cell cytotoxicity in cervical cancer. Rescue experiments using the hedgehog signaling inhibitor GANT58 attenuated the suppressive effect of MYB overexpression on NK cytotoxicity. In summary, MYB inhibited NK cell cytotoxicity by activating the hedgehog signaling pathway in cervical cancer, suggesting its potential as a novel diagnostic marker and immunotherapeutic target.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70084"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LRP8 Regulates Lipid Metabolism to Stimulate Malignant Progression and Cisplatin Resistance in Bladder Cancer. LRP8调节脂质代谢促进膀胱癌恶性进展和顺铂耐药。

IF 3.1

The Kaohsiung journal of medical sciences Pub Date : 2025-09-01 Epub Date: 2025-05-15 DOI: 10.1002/kjm2.70042

Gang-Feng Wu, Zhen-Gang Luo, Ke Gao, Yu Ren, Chong Shen, Xiang-Rong Ying

{"title":"LRP8 Regulates Lipid Metabolism to Stimulate Malignant Progression and Cisplatin Resistance in Bladder Cancer.","authors":"Gang-Feng Wu, Zhen-Gang Luo, Ke Gao, Yu Ren, Chong Shen, Xiang-Rong Ying","doi":"10.1002/kjm2.70042","DOIUrl":"10.1002/kjm2.70042","url":null,"abstract":"Low-density lipoprotein receptor-related protein 8 (LRP8) is a crucial regulator of lipid metabolism and is implicated in the development and treatment of various cancers. However, its role in bladder cancer (BCa) remains unknown. We analyzed LRP8 expression in BCa using the TCGA database and clinical samples. We manipulated LRP8 expression in tumor cell lines using siRNA or overexpression plasmid transfection. Cell proliferation, migration, invasion, apoptosis, and drug resistance were assessed through CCK-8, transwell, flow cytometry, and IC50 assays. Additionally, a rescue experiment confirmed the association between LRP8 and lipid metabolism. LRP8 was significantly upregulated in BCa tissues and cells. Knockdown of LRP8 reduced tumor cell proliferation, migration, invasion, and increased apoptosis while enhancing cisplatin sensitivity. Overexpression of LRP8 boosted malignant progression and cisplatin resistance in tumor cells. The expression level of LRP8 is positively linked with the expression of lipid metabolism-related genes, phospholipid accumulation, and triglyceride accumulation. Notably, inhibiting lipid metabolism reversed the malignant progression and cisplatin resistance induced by LRP8 overexpression. LRP8 could promote BCa malignant progression and cisplatin resistance through lipid metabolism regulation.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70042"},"PeriodicalIF":3.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144083074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0