The Kaohsiung journal of medical sciences最新文献

{"title":"Expression and Clinical Significance of CXCR5 and LAG-3 on Peripheral Blood CD8⁺ T Cells in Patients With Diffuse Large B-Cell Lymphoma.","authors":"Xi-Zhe Guo, Ya-Fei Guo, Shi-Xin Wu","doi":"10.1002/kjm2.70005","DOIUrl":"https://doi.org/10.1002/kjm2.70005","url":null,"abstract":"<p><p>Diffuse large B-cell lymphoma (DLBCL) exhibits substantial biological and clinical heterogeneity. This study investigated the expression and prognostic implications of C-X-C chemokine receptor type 5 (CXCR5) and lymphocyte activation gene-3 (LAG-3) on peripheral blood CD8⁺ T cells in patients with DLBCL. A total of 71 DLBCL patients and 71 healthy controls were enrolled. The expression levels of CXCR5 and LAG-3 on peripheral blood CD8⁺ T cells were assessed and analyzed for their impact on 5-year progression-free survival (PFS) and overall survival (OS). Results revealed significantly elevated CXCR5 and LAG-3 expression levels in DLBCL patients compared to controls. CXCR5 expression correlated with lactate dehydrogenase (LDH) levels, extranodal involvement, Ann Arbor stage, and International Prognostic Index (IPI) scores, while LAG-3 expression was associated with Eastern Cooperative Oncology Group (ECOG) scores, number of extranodal sites, bone marrow involvement, Ann Arbor stage, and IPI scores. Multivariate analysis identified advanced age, Ann Arbor stage III-IV, and elevated CXCR5 and LAG-3 expression as independent risk factors for poorer 5-year PFS and OS. Furthermore, patients with higher CXCR5 and LAG-3 expression levels demonstrated significantly reduced 5-year PFS and OS rates. In conclusion, elevated CXCR5 and LAG-3 expression on peripheral blood CD8⁺ T cells plays a pivotal role in DLBCL progression and prognosis, making these markers potential therapeutic targets or prognostic indicators.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70005"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smoking and Elevated Preneoadjuvant Chemoradiotherapy Serum Carcinoembryonic Antigen Levels Are Associated With High Tumor Regression Grade and Poor Survival in Patients With Locally Advanced Rectal Cancer.","authors":"Jen-Pin Chuang, Hsiang-Lin Tsai, Po-Jung Chen, Ching-Wen Huang, Wei-Chih Su, Tsung-Kun Chang, Yen-Cheng Chen, Ching-Chun Li, Yung-Sung Yeh, Jaw-Yuan Wang","doi":"10.1002/kjm2.70008","DOIUrl":"https://doi.org/10.1002/kjm2.70008","url":null,"abstract":"<p><p>Neoadjuvant chemoradiotherapy (NACRT) is the standard treatment for patients with locally advanced rectal cancer (LARC). Tumor regression grade (TRG) is an essential prognostic factor in determining treatment efficacy. However, the potential factors influencing TRG in patients with rectal cancer who have received NACRT have not been investigated. We conducted a retrospective analysis of patients with LARC who received NACRT followed by surgical resection. We collected data on the patient characteristics, including age, sex, comorbidities, tumor size, lymph node status, time between NACRT and surgery, and pretreatment carcinoembryonic antigen (CEA) levels. TRG was determined on the basis of a pathological assessment of resected specimens, and overall survival (OS) at 5 years was determined. Univariate and multivariate logistic regression models were employed to evaluate the association between the patient characteristics and TRG. Univariate analysis revealed that smoking and prechemoradiotherapy (pre-CRT) and preoperative CEA levels were significantly associated with TRG. In a multivariate analysis, both smoking and higher pre-CRT CEA levels were identified as significant predictors of a high TRG. The hazard ratios were 2.32 (95% confidence interval [CI]: 1.06-5.07, p = 0.036) for smoking and 3.1 (95% CI: 1.69-5.68, p < 0.001) for higher pre-CRT CEA levels. In Kaplan-Meier analysis, the nonsmoker group exhibited higher OS (p = 0.004). Elevated pre-CRT CEA levels and current smoking status were associated with a more than two fold increase in the risk of a higher TRG after NACRT. Moreover, smoking was a significant risk factor for poor OS in patients with LARC following NACRT.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70008"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143618125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PSMD14 Transcriptionally Activated by MEF2A Promotes Pancreatic Cancer Development by Upregulating SPON2 Expression.","authors":"Yun-He Hao, Cheng-Ru Yang, Wu-Jiang Shi, Xiang-Yu Zhong","doi":"10.1002/kjm2.70007","DOIUrl":"https://doi.org/10.1002/kjm2.70007","url":null,"abstract":"<p><p>Proteasome 26S subunit non-ATPase 14 (PSMD14) plays a pro-carcinogenic role in various cancers. However, its specific effects and mechanisms in pancreatic cancer (PC) remain unclear. We aimed to assess the function and mechanism of PSMD14 in PC. Fifteen paired pancreatic ductal adenocarcinoma (PDAC) tissues and adjacent non-tumorous tissues were clinically obtained. Cell proliferation, migration, and invasion were assessed using colony formation, scratch, and Transwell assays. The interaction between the MEF2A transcription factor and the PSMD14 promoter verified by chromatin immunoprecipitation (ChIP) or dual luciferase assay. The interaction between RBM15B and SPON2 mRNA was validated by RNA immunoprecipitation (RIP) assay. The interaction between the proteins PSMD14 and RBM15B was detected by co-immunoprecipitation (Co-IP) assay. The m6A level of SPON2 was detected by methylated RNA immunoprecipitation (MeRIP, a common method for detecting m6A levels of mRNAs). The ubiquitination level of RNA-binding motif protein 15B (RBM15B) was detected using Co-IP. The role of PSMD14 in PC was further explored subcutaneous and lung metastasis models. PSMD14 was upregulated in PDAC tissues. PSMD14 knockdown inhibited PC cell viability, proliferation, migration, and invasion. MEF2A transcriptionally activated PSMD14 expression. PSMD14 knockdown promoted the ubiquitination degradation of RBM15B. Additionally, PSMD14 enhanced SPON2 mRNA stability through RBM15B-mediated m6A modification. SPON2 overexpression impaired the effect of knockdown PSMD14. Finally, PSMD14 knockdown in PC arrested tumor growth and lung metastasis. PSMD14, transcriptionally activated by MEF2A, promotes the de-ubiquitination of RBM15B, which upregulates SPON2 expression in an m6A-RBM15B-dependent manner, thereby facilitating PC proliferation, migration, and invasion.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70007"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA TUSC7 Inhibits Cell Proliferation in Chronic Lymphocytic Leukemia by Modulating the miR-211-5p/SLC37A3 Axis.","authors":"Xu-Li Wang, Jia Mei","doi":"10.1002/kjm2.70003","DOIUrl":"https://doi.org/10.1002/kjm2.70003","url":null,"abstract":"<p><p>Chronic lymphocytic leukemia (CLL) is a malignant lymphoproliferative disorder. Long non-coding RNAs (lncRNAs) have been implicated in various regulatory processes and cancer development. Among these, lncRNA tumor suppressor candidate 7 (TUSC7) has been identified as a tumor suppressor gene. We herein measured TUSC7 expression using RT-qPCR and investigated its biological role in CLL through gain-of-function experiments. Our results revealed that TUSC7 expression was significantly lower in CLL patients compared to healthy controls, and its downregulation was associated with poor prognosis. Meanwhile, TUSC7 overexpression inhibited cell proliferation while promoting cell apoptosis. Mechanistically, TUSC7 interacted with miR-211-5p, thereby regulating the downstream target gene, solute carrier family 37 member 3 (SLC37A3). Further rescue experiments demonstrated that silencing SLC37A3 or upregulating miR-211-5p reversed the effects of TUSC7 elevation on cell proliferation and apoptosis. In conclusion, our findings suggest that TUSC7 regulates cell proliferation in CLL through the miR-211-5p/SLC37A3 axis.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70003"},"PeriodicalIF":0.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143589118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between Serum SFRP5, ApoA-I, HDL3-C Level and In-Stent Restenosis After PCI in Acute Myocardial Infarction and the Combined Predictive Value.","authors":"Li-Qiang Cui, Xue-Dong Wang","doi":"10.1002/kjm2.70000","DOIUrl":"https://doi.org/10.1002/kjm2.70000","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study aims to investigate the relationship between serum secreted frizzled-related protein 5 (SFRP5), apolipoprotein A-I (ApoA-I), high-density lipoprotein 3-cholesterol (HDL3-C) and in-stent restenosis (ISR) after percutaneous coronary intervention (PCI) in acute myocardial infarction (AMI) and their combined predictive value. The clinical data of 128 AMI patients who underwent PCI in our hospital from July 2020 to July 2023 were retrospectively analyzed. After 12 months of follow-up, the patients were divided into the ISR group (24 cases) and the non-ISR group (104 cases) according to the results of coronary angiography. The 24 patients with ISR were divided into Grade III (lumen stenosis area of 50%-70%, 15 cases) and Grade IV (lumen stenosis area of 76%-100%, 9 cases). The general data of the two groups were compared. The serum levels of SFRP5, ApoA-I, and HDL3-C in the two groups were analyzed on the second day after the operation. The levels of SFRP5, ApoA-I, and HDL3-C in patients with different degrees of stenosis were compared. The correlation between serum SFRP5, ApoA-I, HDL3-C levels and ISR after PCI was analyzed by bivariate correlation Kendall tau-b (K). Logistic regression was used to analyze the influencing factors of ISR after PCI. The receiver operating characteristic (ROC) curve was drawn to analyze the predictive value of SFRP5, ApoA-I, and HDL3-C in ISR after PCI. The proportion of patients with diabetes and a smoking history in the ISR group was higher than that in the non-ISR group. The stent length (29.52 ± 5.47 mm) and hs-CRP level (3.38 ± 0.51 mg/L) in the ISR group were higher than those in the non-ISR group (23.56 ± 5.37 mm and 2.78 ± 0.52 mg/L) (p &lt; 0.05). SFRP5 (15.33 ± 2.60 ng/mL), ApoA-I (1.22 ± 0.37 g/L) and HDL3-C (0.31 ± 0.07 mmol/L) in the ISR group were higher than those in the non-ISR group (19.79 ± 3.09 ng/mL, 1.77 ± 0.41 g/L, and 0.46 ± 0.11 mmol/L) (p &lt; 0.001). The levels of SFRP5 (17.57 ± 2.57 ng/mL), ApoA-I (1.56 ± 0.34 g/L) and HDL3-C (0.36 ± 0.07 mmol/L) in the Grade III group were higher than those in the Grade IV group (13.15 ± 2.35 ng/mL, 0.98 ± 0.20 g/L, and 0.25 ± 0.05 mmol/L) (p &lt; 0.05). The results of bivariate correlation Kendall tau-b (K) analysis showed that the levels of serum SFRP5, ApoA-I, and HDL3-C were negatively correlated with ISR (r &lt; 0, p &lt; 0.05). Logistic regression analysis showed that diabetes and hs-CRP were risk factors for ISR after PCI (OR &gt; 1, p &lt; 0.05). SFRP5, ApoA-I, and HDL3-C were protective factors for ISR after PCI (OR &lt; 1, p &lt; 0.05). The ROC curve showed that the AUC of SFRP5, ApoA-I, and HDL3-C levels alone and in combination to predict ISR in AMI patients after PCI was &gt; 0.70, which had certain predictive value, and the combined value was higher. In conclusion, diabetes and high levels of hs-CRP were risk factors for ISR in patients with AMI after PCI. High levels of SFRP5, ApoA-I, and HDL3-C were protective factors for ISR after PCI, and their combined d","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70000"},"PeriodicalIF":0.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143589120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA SNHG14 Delivered by Bone Marrow Mesenchymal Stem Cells-Secreted Exosomes Regulates Osteogenesis and Adipogenesis in Osteoporosis by Mediating the miR-27a-3p/LMNB1 Axis.","authors":"Jin-Shan Tang, Huai-Xi Yu, Ru-Xin Ruan, Rui Chen, Zi-Qiang Zhu","doi":"10.1002/kjm2.70004","DOIUrl":"https://doi.org/10.1002/kjm2.70004","url":null,"abstract":"<p><p>The purpose of this study was to investigate the role of LncRNA SNHG14 delivered by bone marrow mesenchymal stem cells-secreted exosomes (BMSC-Exos) in osteoporosis (OP). BMSCs and BMSCs-Exos were isolated and identified. BMSCs were transfected, from which BMSCs-Exos were collected. The treated BMSCs-Exos were co-cultured with BMSCs. After osteogenic induction of BMSCs, the calcification was analyzed by alizarin red S staining. After adipogenic induction of BMSCs, lipid droplets were detected by oil red O staining. Glycerol-3-phosphate dehydrogenase activity was measured in BMSCs. OVX mouse models were established and treated with BMSC-EXOs. HE staining and IHC staining were performed on the femurs of mice. The interaction between SNHG14, miR-27a-3p, and LMNB1 was evaluated by luciferase reporter gene assay and RIP assay. Gene levels were assessed using RT-qPCR and Western blot, respectively. BMSC-Exos promoted osteogenic-adipogenic balance of BMSCs. SNHG14 enhanced the promoting effect of BMSCs-Exos on the balance of osteogenesis and adipogenesis of BMSCs. SNHG14 directly bound miR-27a-3p. SNHG1 mediated osteogenic-adipogenic balance in BMSCs via miR-27a-3p. LMNB1 was a target gene of miR-27a-3p. LMNB1 was involved in the process of SNHG14 regulating osteogenic-adipogenic balance in BMSCs. SNHG14 overexpression promoted bone formation and alleviated OP in vivo. SNHG14 delivered by BMSCs-Exos regulates osteogenesis and adipogenesis in OP by mediating the miR-27a-3p/LMNB1 axis.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70004"},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Role of miR-490-3p in Blocking Bladder Cancer Growth Through Targeting the RNA-Binding Protein PCBP2.","authors":"","doi":"10.1002/kjm2.70009","DOIUrl":"https://doi.org/10.1002/kjm2.70009","url":null,"abstract":"<p><strong>Retraction: </strong>C.-M. Zhang, L.-D. Song, J.-W. Wang, H.-B. Ye, and S. Chen, \"Role of miR-490-3p in Blocking Bladder Cancer Growth Through Targeting the RNA-Binding Protein PCBP2,\" Kaohsiung Journal of Medical Sciences 38, no. 1 (2022): 30-37. https://doi.org/10.1002/kjm2.12457. The above article, published online on 07 October 2021, in Wiley Online Library (wileyonlinelibrary.com), and has been retracted by agreement between the journal Editor-in-Chief, Wan-Long Chuang; Kaohsiung Medical University; and John Wiley and Sons Australia Ltd. The authors reported to the journal that further experiments with miR-490-3p mimic showed different results for cell viability than those that were presented in the published article. The authors further stated that these new results indicate that there are further errors in the results presented. Therefore, all parties agree that the article must be retracted. The authors did not respond to the notice regarding the retraction of their article.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70009"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143544963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intractable diarrhea caused by a rare rectal foreign body.","authors":"Qi Fan, Shuang Yu, De-Hai Xiong","doi":"10.1002/kjm2.12917","DOIUrl":"https://doi.org/10.1002/kjm2.12917","url":null,"abstract":"","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":"41 3","pages":"e12917"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis C virus-free endoscope procedures project: An in-hospital elimination approach.","authors":"Benjamin Lih-Ren Hsieh, Yen-Ting Kuo, Yu-Ju Wei, Pei-Chien Tsai, Ming-Lun Yeh, Chung-Feng Huang, Chia-Yen Dai, Ming-Yen Hsieh, Jee-Fu Huang, Ming-Lung Yu, Wan-Long Chuang","doi":"10.1002/kjm2.12942","DOIUrl":"10.1002/kjm2.12942","url":null,"abstract":"<p><p>Hepatitis C virus (HCV) elimination in the care cascades for patients receiving invasive procedures remains elusive. This study aimed to evaluate the efficacy of HCV-free Endoscope Procedures Project (CEPP) in the effort toward hospital HCV micro-elimination in Taiwan. An electronic medical record (EMR)-based remind system was introduced into gastrointestinal, surgical, urological, and gynecological departments prior to the endoscopy procedures. Anti-HCV tests were actively ordered on their EMR among those patients who have not been tested in the past 5 years. Those patients with anti-HCV+ were recruited into the care cascade for HCV treatment, including HCVRNA testing, direct antivirals (DAAs) delivery, and treatment response assessment. We divided the elimination project into two phases: before (2020 Jan to 2020 Dec, phase A) and during (2021 Jan to 2022 Sep, phase B) the remind system. The screening rate of phase B was 64.2% (1857/2893), which was significantly higher than phase A (18.7%, 899/4812) (p < 0.001). The screening rate of Department of Medicine (DOM) significantly increased from 21.1% of phase A to 89.3% of Phase B (p < 0.001). During phase B, the screening rate of non-DOM was 48.2%, which was significantly higher than 11.8% of Phase A (p < 0.001). During Phase B, 15 (0.8%) out of 1857 screened patients were HCVRNA+. Six HCVRNA+ patients received DAAs treatment, and all achieved viral eradication. The CEPP significantly increased the anti-HCV screening rate for subsequent care cascades, particularly in patients of DOM.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e12942"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of platelet-rich plasma and platelet-rich fibrin on healing of burn wound with dual-species biofilm.","authors":"Wen-Dan Li, Fang Lin, Yu Sun, Zi-Jing Zhu, Mei-Liang Luo, Yi-Qi Zeng, Zhen Lin, Mou Zhou","doi":"10.1002/kjm2.12940","DOIUrl":"10.1002/kjm2.12940","url":null,"abstract":"<p><p>This study evaluated the impact of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on burn wound with dual-species biofilm. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) were applied to infect the burn wound in rats to establish a dual-species biofilm model. After infection, the wound was treated with ionized silver (AG), PRF, and PRP. Silver scanning electron microscopy (SEM) was used to assess adhesion after infection. PRF and PRP reduced wound size from day 8 after burn injuries, while AG significantly promoted burn wound healing at day 12. New collagen was formed in the shortest time in PRF and PRP groups compared to AG and control groups. PRF and PRP greatly lowered the bacterial numbers in wounds with S. aureus and P. aeruginosa biofilm, whereas AG showed weak bacteriostatic effects. AG, PRF, and PRP treatments significantly reduced inflammatory mediators and induced VEGFA. However, AG treatment increased TNF-α. PRF and PRP accelerate wound healing in the presence of dual-species biofilm infection and show strong antibacterial activity against S. aureus and P. aeruginosa, indicating that PRF and PRP could be potential therapies for burn wounds with dual-species biofilm infection.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e12940"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}