The Kaohsiung journal of medical sciences最新文献_第10页

Liquid silicone gel injection leading to primary squamous cell carcinoma of the breast. 液体硅凝胶注射导致乳腺原发性鳞状细胞癌。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1002/kjm2.12874

Hidenobu Takahashi, Yen-Shuo Huang, Chee-Yin Chai, Jung-Yu Kan

引用次数: 0

Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury. 增强治疗潜力：用表达 VEGF165 基因的重组腺相关病毒修饰的人脂肪间充质干细胞治疗周围神经损伤。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-08-05 DOI: 10.1002/kjm2.12875

Shuai Jiang, Bo Chen, Zhen-Yu Sun

{"title":"Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.","authors":"Shuai Jiang, Bo Chen, Zhen-Yu Sun","doi":"10.1002/kjm2.12875","DOIUrl":"10.1002/kjm2.12875","url":null,"abstract":"This study aimed to investigate the therapeutic potential of human adipose-derived mesenchymal stem cells (hADSCs) modified with recombinant adeno-associated virus (rAAV) carrying the vascular endothelial growth factor 165 (VEGF165) gene in peripheral nerve injury (PNI). The hADSCs were categorized into blank, control (transduced with rAAV control vector), and VEGF165 (transduced with rAAV VEGF165 vector) groups. Subsequently, Schwann cell differentiation was induced, and Schwann cell markers were assessed. The sciatic nerve injury mouse model received injections of phosphate-buffered saline (PBS group), PBS containing hADSCs (hADSCs group), rAAV control vector (control-hADSCs group), or rAAV VEGF165 vector (VEGF165-hADSCs group) into the nerve defect site. Motor function recovery, evaluated through the sciatic function index (SFI), and nerve regeneration, assessed via toluidine blue staining along with scrutiny of Schwann cell markers and neurotrophic factors, were conducted. Modified hADSCs exhibited enhanced Schwann cell differentiation and elevated expression of Schwann cell markers [S100 calcium-binding protein B (S100B), NGF receptor (NGFR), and glial fibrillary acidic protein (GFAP)]. Mice in the VEGF165-hADSCs group demonstrated improved motor function recovery compared to those in the other three groups, accompanied by increased fiber diameter, axon diameter, and myelin thickness, as well as elevated expression of Schwann cell markers (S100B, NGFR, and GFAP) and neurotrophic factors [mature brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF)] in the distal nerve segment. rAAV-VEGF165 modification enhances hADSC potential in PNI, promoting motor recovery and nerve regeneration. Elevated Schwann cell markers and neurotrophic factors underscore therapy benefits, providing insights for nerve injury strategies.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"819-829"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the diagnostic potential of immunoglobulin A-microbiota interplay in liver cirrhosis and spontaneous bacterial peritonitis. 探索肝硬化和自发性细菌性腹膜炎中免疫球蛋白 A-微生物群相互作用的诊断潜力。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-18 DOI: 10.1002/kjm2.12876

Liang-Jie Zhang, Wen-Qi Huang, Yuan Zhang, You-Lian Zhou, Hao-Ming Xu, Chong Zhao, Yu-Qiang Nie

{"title":"Exploring the diagnostic potential of immunoglobulin A-microbiota interplay in liver cirrhosis and spontaneous bacterial peritonitis.","authors":"Liang-Jie Zhang, Wen-Qi Huang, Yuan Zhang, You-Lian Zhou, Hao-Ming Xu, Chong Zhao, Yu-Qiang Nie","doi":"10.1002/kjm2.12876","DOIUrl":"10.1002/kjm2.12876","url":null,"abstract":"The human gut microbiota significantly impacts health, including liver conditions like liver cirrhosis (LC) and spontaneous bacterial peritonitis (SBP). Immunoglobulin A (IgA) plays a central role in maintaining gut microbial balance. Understanding IgA's interplay with gut microbiota and liver health is crucial. This study explores the relationship between fecal IgA levels, gut microbiota, and liver injury severity. A total of 69 LC patients and 30 healthy controls were studied. Fecal IgA levels were measured using ELISA, and IgA-coated bacteria were quantified via flow cytometry. Microbiota diversity and composition were assessed through 16S rRNA sequencing. Liver injury severity was graded using the Child-Pugh score. Statistical analyses determined correlations. LC patients had higher fecal IgA levels than controls, correlating positively with liver injury severity. Microbiota diversity decreased with severity, accompanied by shifts in composition favoring pro-inflammatory species. Ralstonia abundance positively correlated with liver injury, whereas Faecalibacterium showed a negative correlation. Specific microbial markers for SBP were identified. Functional profiling revealed altered microbial functionalities in LC and SBP. Elevated fecal IgA levels, coupled with microbiota alterations, correlate with liver injury severity in LC patients. Modulating gut microbiota could be a promising strategy for managing liver-related conditions. Further research is needed to understand underlying mechanisms and translate findings into clinical practice, potentially improving patient outcomes.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"837-851"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of gut microbiota on morbidities in preterm infants. 肠道微生物群对早产儿发病率的影响。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1002/kjm2.12878

Mei-Yin Lai, Yin-Hsi Chang, Chien-Chung Lee

{"title":"The impact of gut microbiota on morbidities in preterm infants.","authors":"Mei-Yin Lai, Yin-Hsi Chang, Chien-Chung Lee","doi":"10.1002/kjm2.12878","DOIUrl":"10.1002/kjm2.12878","url":null,"abstract":"The gut microbiota undergoes substantial development from birth, and its development in the initial years of life has a potentially lifelong effect on the health of the individual. However, various factors can disrupt the development of the gut microbiota, leading to a condition known as dysbiosis, particularly in preterm infants. Current studies involving adults have suggested that the gut microbiota not only influences the gut but also has multidimensional effects on remote organs; these pathways are often referred to as the gut-organ axis. Imbalance of the gut microbiota may lead to the development of multiple diseases. Recent studies have revealed that gut dysbiosis in preterm infants may cause several acute morbidities-such as necrotizing enterocolitis, late-onset sepsis, bronchopulmonary dysplasia, and retinopathy of prematurity-and it may also influence long-term outcomes including neurodevelopment and somatic growth. This review mainly presents the existing evidence regarding the relationships between the gut microbiota and these morbidities in preterm infants and explores the role of the gut-organ axis in these morbidities. This paper thus offers insights into the future perspectives on microbiota interventions for promoting the health of preterm infants.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"780-788"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decision-making processes in artificial intelligence applications in dentomaxillofacial radiology from the perspective of Wittgenstein. 从维特根斯坦的角度看牙颌面放射学中人工智能应用的决策过程。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-19 DOI: 10.1002/kjm2.12877

Sumeyye Celik Ozsoy, Samed Satir, Usame Omer Osmanoglu

引用次数: 0

Differential expression of host oncogenes in human papillomavirus-associated nasopharyngeal and cervical epithelial cancers. 人乳头瘤病毒相关鼻咽癌和宫颈上皮癌中宿主癌基因的差异表达。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1002/kjm2.12880

Santa Sheila, Brown Charles Adoquaye, Akakpo Patrick Kafui, Edusei Lawrence, Hooper Andrew Richard, Quaye Osbourne, Tagoe Emmanuel Ayitey

{"title":"Differential expression of host oncogenes in human papillomavirus-associated nasopharyngeal and cervical epithelial cancers.","authors":"Santa Sheila, Brown Charles Adoquaye, Akakpo Patrick Kafui, Edusei Lawrence, Hooper Andrew Richard, Quaye Osbourne, Tagoe Emmanuel Ayitey","doi":"10.1002/kjm2.12880","DOIUrl":"10.1002/kjm2.12880","url":null,"abstract":"Human papillomavirus (HPV)-related cervical and nasopharyngeal cancers differ in molecular mechanisms underlying the oncogenic processes. The disparity may be attributed to differential expression of oncoproteins. The current study investigated the host oncogenes expression pattern in HPV-associated cervical and nasopharyngeal cancer. Formalin-fixed paraffin-embedded tissues originating from the nasopharyngeal and cervical regions were screened using Hematoxylin and Eosin staining. Genomic DNA and total RNA were extracted from confirmed cancer biopsies and non-cancer tissues (NC). HPV was detected by PCR using MY09/GP5+/6+ primers. Protein expression levels of AKT, IQGAP1, and MMP16 in HPV-infected cancers and controls were determined by immunohistochemistry. RT-qPCR was used to profile mRNAs of the oncogenes. AKT and IQGAP1 proteins were highly expressed in the epithelial cancers compared with the non-cancer tissues (p < 0.05). IQGAP1 and MMP16 mRNAs level was significantly higher in the cancers than in the NC (p < 0.05), but not AKT mRNA levels. MMP16 protein was ubiquitously expressed in all tissues. AKT mRNA level was significantly elevated in CC compared with NPC (p < 0.001). However, the difference in AKT, IQGAP1 and MMP16 proteins level between CC and NPC was not significant (p > 0.05). The oncoproteins expression level between the HPV-positive and HPV-negative cancer biopsies showed no significant difference (p < 0.05). Current study reports AKT but not IQGAP1 and MMP16 mRNAs differentially expression in cervical and nasopharyngeal cancers, independent of HPV infection status.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"830-836"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral hepatitis moderates the impact of TGFB1 on neurocognitive impairment. 病毒性肝炎可调节 TGFB1 对神经认知障碍的影响。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-06 DOI: 10.1002/kjm2.12872

Wei-Chia Tsao, Rwei-Ling Yu, Chi-Ting Li, Wei-Fang Tsai, Wan-Long Chuang, Jee-Fu Huang, Chia-Yen Dai, Chun-Hsiang Tan

{"title":"Viral hepatitis moderates the impact of TGFB1 on neurocognitive impairment.","authors":"Wei-Chia Tsao, Rwei-Ling Yu, Chi-Ting Li, Wei-Fang Tsai, Wan-Long Chuang, Jee-Fu Huang, Chia-Yen Dai, Chun-Hsiang Tan","doi":"10.1002/kjm2.12872","DOIUrl":"10.1002/kjm2.12872","url":null,"abstract":"Recent studies have identified a correlation between chronic viral hepatitis and cognitive impairment, yet the underlying mechanisms remain unclear. This study investigated the influence of TGFB1 genetic polymorphisms on cognitive function in individuals with and without hepatitis infections, hypothesizing that these polymorphisms and the viral hepatitis-induced inflammatory environment interact to affect cognitive abilities. Participants (173 with viral hepatitis and 258 healthy controls) were recruited. Genotyping of TGFB1 SNPs was performed using the C2-58 Axiom Genome-Wide TWB 2.0 Array Plate. Cognitive function was assessed using the MMSE and MoCA tests. Our results showed that healthy individuals carrying the C allele of rs2241715 displayed better performance in sentence writing (p = 0.020) and language tasks (p = 0.022). Notably, viral hepatitis was found to moderate the impact of the rs2241715 genotype on language function (p = 0.002). Similarly, those carrying the T allele of rs10417924 demonstrated superior orientation to time (p = 0.002), with viral hepatitis modifying the influence of the SNP on this particular cognitive function (p = 0.010). Our findings underscore the significant role of TGFβ1 in cognitive function and the moderating impact of viral hepatitis on TGFB1 SNP effects. These findings illuminate the potential of TGFB1 as a therapeutic target for cognitive impairment induced by viral hepatitis, thus broadening our understanding of TGFβ1 functionality in the pathogenesis of neurodegeneration.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"852-861"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141545735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N6-methyladenosine-mediated LINC01087 promotes lung adenocarcinoma progression by regulating miR-514a-3p to upregulate centrosome protein 55. N6-甲基腺苷介导的 LINC01087 通过调控 miR-514a-3p 上调中心体蛋白 55 促进肺腺癌的进展。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-07-18 DOI: 10.1002/kjm2.12879

Xin Zhang, Dong-Jie Wang, Li Jia, Wei Zhang

{"title":"N6-methyladenosine-mediated LINC01087 promotes lung adenocarcinoma progression by regulating miR-514a-3p to upregulate centrosome protein 55.","authors":"Xin Zhang, Dong-Jie Wang, Li Jia, Wei Zhang","doi":"10.1002/kjm2.12879","DOIUrl":"10.1002/kjm2.12879","url":null,"abstract":"Long noncoding RNAs are key players in the development of lung adenocarcinoma (LUAD). The present study elucidated the role of LINC01087 in LUAD development. Cell vitality and apoptosis were assessed by the CCK-8 assay and flow cytometry, respectively. The transwell assay was adopted to evaluate cell migration and invasion. Levels of m6A modification of LINC01087 were determined using the methylated RNA binding protein immunoprecipitation assay. The interactions among LINC01087, miR-514a-3p, and centrosome protein 55 (CEP55) were evaluated using dual-luciferase reporter, RNA immunoprecipitation, and RNA-RNA pull-down assays. LINC01087 was highly expressed in LUAD, and its downregulation restrained cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition in vitro as well as tumor growth in a xenograft tumor model. Overexpression of miR-514a-3p inhibited malignant phenotypes in LUAD cells by inactivating RhoA/ROCK1 signaling via the suppression of CEP55 expression. Mechanistically, RBM15 increased the expression and mRNA stability of LINC01087 by mediating its m6A modification and LINC01087 induced CEP55 expression by sponging miR-514a-3p. RBM15-induced LINC01087 upregulation accelerated LUAD progression by regulating the miR-514a-3p/CEP55/RhoA/ROCK1 axis, illustrating the potential of LINC01087 as a novel target for LUAD therapy.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"801-818"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to "LncRNA MYLK antisense RNA 1 activates cell division cycle 42/Neutal Wiskott-Aldrich syndrome protein pathway via microRNA-101-5p to accelerate epithelial-to-mesenchymal transition of colon cancer cells". 更正 "LncRNA MYLK反义RNA 1通过microRNA-101-5p激活细胞分裂周期42/中性维斯科特-阿尔德里奇综合征蛋白通路，加速结肠癌细胞上皮细胞向间质转化"。

The Kaohsiung journal of medical sciences Pub Date : 2024-09-01 Epub Date: 2024-09-04 DOI: 10.1002/kjm2.12893

引用次数: 0

Development of a diagnostic support system for the fibrosis of nonalcoholic fatty liver disease using artificial intelligence and deep learning. 利用人工智能和深度学习开发非酒精性脂肪肝纤维化诊断支持系统。

The Kaohsiung journal of medical sciences Pub Date : 2024-08-01 Epub Date: 2024-05-31 DOI: 10.1002/kjm2.12850

Noppamate Preechathammawong, Mongkon Charoenpitakchai, Nutthawat Wongsason, Julalak Karuehardsuwan, Thaninee Prasoppokakorn, Panyavee Pitisuttithum, Anapat Sanpavat, Karn Yongsiriwit, Thannob Aribarg, Parkpoom Chaisiriprasert, Sombat Treeprasertsuk, Sakkarin Chirapongsathorn

{"title":"Development of a diagnostic support system for the fibrosis of nonalcoholic fatty liver disease using artificial intelligence and deep learning.","authors":"Noppamate Preechathammawong, Mongkon Charoenpitakchai, Nutthawat Wongsason, Julalak Karuehardsuwan, Thaninee Prasoppokakorn, Panyavee Pitisuttithum, Anapat Sanpavat, Karn Yongsiriwit, Thannob Aribarg, Parkpoom Chaisiriprasert, Sombat Treeprasertsuk, Sakkarin Chirapongsathorn","doi":"10.1002/kjm2.12850","DOIUrl":"10.1002/kjm2.12850","url":null,"abstract":"Liver fibrosis is a pathological condition characterized by the abnormal proliferation of liver tissue, subsequently able to progress to cirrhosis or possibly hepatocellular carcinoma. The development of artificial intelligence and deep learning have begun to play a significant role in fibrosis detection. This study aimed to develop SMART AI-PATHO, a fully automated assessment method combining quantification of histopathological architectural features, to analyze steatosis and fibrosis in nonalcoholic fatty liver disease (NAFLD) core biopsies and employ Metavir fibrosis staging as standard references and fat assessment grading measurement for comparison with the pathologist interpretations. There were 146 participants enrolled in our study. The correlation of Metavir scoring system interpretation between pathologists and SMART AI-PATHO was significantly correlated (Agreement = 68%, Kappa = 0.59, p-value <0.001), which subgroup analysis of significant fibrosis (Metavir score F2-F4) and nonsignificant fibrosis (Metavir score F0-F1) demonstrated substantial correlated results (agreement = 80%, kappa = 0.61, p-value <0.001), corresponding with the correlation of advanced fibrosis (Metavir score F3-F4) and nonadvanced fibrosis groups (Metavir score F0-F2), (agreement = 89%, kappa = 0.74, p-value <0.001). SMART AI-PATHO, the first pivotal artificially intelligent diagnostic tool for the color-based NAFLD hepatic tissue staging in Thailand, demonstrated satisfactory performance as a pathologist to provide liver fibrosis scoring and steatosis grading. In the future, developing AI algorithms and reliable testing on a larger scale may increase accuracy and contribute to telemedicine consultations for general pathologists in clinical practice.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"757-765"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141181786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0