The Kaohsiung journal of medical sciences最新文献_第2页

Multiple Gastric Adenomyomas Mimicking Leiomyoma: A Novel Case Report.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-31 DOI: 10.1002/kjm2.70018

Chen Yuan, You-Hong Cao

引用次数: 0

Circulating let-7 Predicts Hepatic Fibrogenesis of 12-Month Post-Nucleos(t)ide Analog Treatment in Patients With Hepatitis B Virus. 循环let-7可预测乙型肝炎病毒感染者核苷酸类似物治疗后12个月的肝纤维化情况

The Kaohsiung journal of medical sciences Pub Date : 2025-03-29 DOI: 10.1002/kjm2.70015

Yi-Shan Tsai, Po-Cheng Liang, Yi-Hung Lin, Tyng-Yuan Jang, Yu-Ju Wei, Po-Han Chen, Jia-Ning Hsu, Meng-Hsuan Hsieh, Ming-Yen Hsieh, Chih-Wen Wang, Zu-Yau Lin, Ming-Lun Yeh, Chung-Feng Huang, Jee-Fu Huang, Ming-Lung Yu, Wan-Long Chuang, Chia-Yen Dai

{"title":"Circulating let-7 Predicts Hepatic Fibrogenesis of 12-Month Post-Nucleos(t)ide Analog Treatment in Patients With Hepatitis B Virus.","authors":"Yi-Shan Tsai, Po-Cheng Liang, Yi-Hung Lin, Tyng-Yuan Jang, Yu-Ju Wei, Po-Han Chen, Jia-Ning Hsu, Meng-Hsuan Hsieh, Ming-Yen Hsieh, Chih-Wen Wang, Zu-Yau Lin, Ming-Lun Yeh, Chung-Feng Huang, Jee-Fu Huang, Ming-Lung Yu, Wan-Long Chuang, Chia-Yen Dai","doi":"10.1002/kjm2.70015","DOIUrl":"https://doi.org/10.1002/kjm2.70015","url":null,"abstract":"Chronic hepatitis B virus (HBV) infection is associated with potential complications of liver cirrhosis and hepatocellular carcinoma. To date, there are no effective and noninvasive clinical markers that can predict the risk of liver fibrosis early and accurately in chronic hepatitis B (CHB) patients treated with nucleos(t)ide analogs (NAs). This study aimed to investigate the association of circulating let-7b/c/g levels with the severity of hepatic fibrosis with a FIB-4 index of 1.5-2.9 in CHB patients. We conducted a retrospective longitudinal study in patients with CHB after 6 months of NAs therapy to investigate whether serum let-7b/c/g levels can be monitored as an early biomarker for liver fibrogenesis based on multivariate logistic regression analyses. We also used the hepatic stellate cell line LX-2 treated with transforming growth factor-β (TGF-β) to evaluate the suppression effect of let-7b/c/g on hepatic fibrogenesis. The study showed that circulating let-7b/c/g could predict 12 months of antiviral treatment for HBV-related significant fibrosis (FIB-4 index ≥ 2.9) at baseline and was significantly negatively correlated with the FIB-4 score. Moreover, let-7b/c/g could directly target the TGF-βR1-3' untranslated region (3' UTR) and inhibit TGF-β induced p-SMAD2 phosphorylation to reduce α-smooth muscle actin levels, a fibrogenesis marker in LX-2 cells. These results confirm that let-7b/c/g could be a biomarker for monitoring HBV-induced fibrogenesis.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70015"},"PeriodicalIF":0.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of Metastasis in Small Pulmonary Oligonodules Detected in Breast Cancer Patients at Baseline CT.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-27 DOI: 10.1002/kjm2.70014

Huei-Yi Tsai, Min-Fang Chao, Tzu-Hsueh Tsai, Shen-Liang Shih, Fang-Ming Chen, Ming-Feng Hou, Ming-Yii Huang, Jui-Sheng Hsu

{"title":"Prediction of Metastasis in Small Pulmonary Oligonodules Detected in Breast Cancer Patients at Baseline CT.","authors":"Huei-Yi Tsai, Min-Fang Chao, Tzu-Hsueh Tsai, Shen-Liang Shih, Fang-Ming Chen, Ming-Feng Hou, Ming-Yii Huang, Jui-Sheng Hsu","doi":"10.1002/kjm2.70014","DOIUrl":"https://doi.org/10.1002/kjm2.70014","url":null,"abstract":"It is challenging for radiologists to diagnose pulmonary metastases when they encounter only a few (n ≤ 5) small pulmonary nodules (< 10 mm) on staging CT in breast cancer patients. We conducted this study to assess clinical and imaging features related to metastasis for better risk stratification. Retrospective analysis of 249 pulmonary nodules present at the baseline CTs of 194 patients diagnosed with breast cancer between 2014 and 2021 was performed. The evaluated features included nodular characteristics, perifissural nodules, associated imaging findings, clinical stage, and breast cancer subtype. Nodules with interval change were determined to be metastases. A large proportion of the patients had single nodule (78.9%) presence, and most of the nodules were less than 6 mm (86.3%). Among the 249 nodules, 63 (25.3%) nodules were in metastases. The independent predictors were nodule ≥ 6 mm, mediastinal/hilar lymphadenopathy, clinical Stages III and IV, and triple-negative breast cancer subtype. Nodules (≥ 6 mm) were assessed as weak evidence to rule in metastasis, and the results were as follows: positive likelihood ratio (+LR), 3.74; sensitivity, 30.2%; and specificity, 91.9%. With weak evidence of small pulmonary nodules (≧ 6 mm) to rule in metastases, it may be appropriate to follow the recommendations of growing nodule management. By contrast, the nodular shape, margin, location, perifissural nodules, and pleural tag did not show an association with metastasis.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70014"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adult Acute Megakaryoblastic Leukemia in a Mantle Cell Lymphoma Post Treatment and Auto-Transplant.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-26 DOI: 10.1002/kjm2.70013

Chin-Mu Hsu, Bi-Hua Du, Chien Hsiao, Hui-Hua Hsiao

引用次数: 0

Erythroderma Triggered by Cutaneous Fungal Infection and Prolonged Steroid Use: A Lesson Learned.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-24 DOI: 10.1002/kjm2.70012

Chin-Yu Lee, Wei-Cheng Fang, Yue-Chiu Su, Yang-Yi Chen

引用次数: 0

EP300 Modulates MCM8 Transcription and Augments the Malignant Phenotype of Hepatitis B Virus-Positive Hepatocellular Carcinoma Cells.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-17 DOI: 10.1002/kjm2.70006

Fang Xue, Tian-Feng Sun

{"title":"EP300 Modulates MCM8 Transcription and Augments the Malignant Phenotype of Hepatitis B Virus-Positive Hepatocellular Carcinoma Cells.","authors":"Fang Xue, Tian-Feng Sun","doi":"10.1002/kjm2.70006","DOIUrl":"https://doi.org/10.1002/kjm2.70006","url":null,"abstract":"Chronic infection with the hepatitis B virus (HBV) remains one of the primary drivers of the development of hepatocellular carcinoma (HCC), a highly aggressive malignancy with a grim prognosis. This study focused on the role of E1A-binding protein p300 (EP300) in the malignant phenotype of HBV-positive HCC cells and its functional mechanism. Increased EP300 expression was detected in HBV-positive tumor tissues and cells compared to their control counterparts. Silencing EP300 reduced tumorigenic activity, proliferation, viability, migration, invasion, and resistance to apoptosis of HBV-positive cells and reduced the concentrations of HBV infection markers HBsAg and HBeAg. These effects were achieved, at least in part, through downregulation of minichromosome maintenance 8 homologous recombination repair factor (MCM8). MCM8 was identified as a target of EP300 and mediated by acetylation modification. MCM8 was upregulated in HBV-positive tumors and HCC cells while decreasing following EP300 silencing in cells. However, the restoration of MCM8 expression in these cells rescued their malignant properties. In summary, this study suggests a role for EP300-mediated MCM8 upregulation in the malignant properties of HBV-positive HCC.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70006"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Clostridioides difficile Infection on Clinical Outcomes in Hospitalized IBD Patients and the Role of Fecal Microbiota Transplantation: A Retrospective Cohort Study.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-17 DOI: 10.1002/kjm2.70002

Puo-Hsien Le, Chyi-Liang Chen, Chia-Jung Kuo, Pai-Jui Yeh, Chien-Chang Chen, Yi-Ching Chen, Cheng-Tang Chiu, Hao-Tsai Cheng, Yung-Kuan Tsou, Yu-Bin Pan, Cheng-Hsun Chiu

{"title":"Impact of Clostridioides difficile Infection on Clinical Outcomes in Hospitalized IBD Patients and the Role of Fecal Microbiota Transplantation: A Retrospective Cohort Study.","authors":"Puo-Hsien Le, Chyi-Liang Chen, Chia-Jung Kuo, Pai-Jui Yeh, Chien-Chang Chen, Yi-Ching Chen, Cheng-Tang Chiu, Hao-Tsai Cheng, Yung-Kuan Tsou, Yu-Bin Pan, Cheng-Hsun Chiu","doi":"10.1002/kjm2.70002","DOIUrl":"https://doi.org/10.1002/kjm2.70002","url":null,"abstract":"Clostridioides difficile infection (CDI) worsens the prognosis of patients with inflammatory bowel disease (IBD). This retrospective cohort study aimed to evaluate the risk factors, clinical manifestations, and outcomes of CDI in hospitalized patients with IBD, including those with toxin A/B results between April 2007 and April 2021. Patients were classified into the CDI and control groups. Patients with IBD and recurrent or refractory CDI underwent fecal microbiota transplantation (FMT). A total of 144 inpatients with IBD-45 in the CDI group and 99 in the control group-were analyzed. The incidence of CDI in inpatients with IBD was 31%. The Risk factors for CDI included longer IBD duration, biological therapy failure, and biological use. More patients in the CDI group presented with abdominal pain (77.8% vs. 55.6%, p = 0.011). In the antibiotic treatment-only group, the symptom improvement rate was 60.7% (17/28), the microbiological cure rate was 89.3% (25/28), and the overall success rate was 71.4% (20/28). After antibiotic treatment and FMT, 71.4% (10/14) of the patients tested negative for CDI, and 64.3% (9/14) had improved clinical symptoms. CDI led to more hospitalizations (median two times [range 0-12] vs. median one time [range 0-19], p = 0.008), a lower steroid-free remission rate (46.7% vs. 67.7%, p = 0.017), and higher Mayo scores (median 5 points [range 2-12] vs. median 3 points [range 0-12]). Patients who received FMT had fewer hospitalizations and fewer IBD-related complications during follow-up than those who received antibiotics alone. FMT should be considered in patients with IBD with refractory or recurrent CDI to improve clinical outcomes.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70002"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Clinical Significance of CXCR5 and LAG-3 on Peripheral Blood CD8⁺ T Cells in Patients With Diffuse Large B-Cell Lymphoma.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-17 DOI: 10.1002/kjm2.70005

Xi-Zhe Guo, Ya-Fei Guo, Shi-Xin Wu

{"title":"Expression and Clinical Significance of CXCR5 and LAG-3 on Peripheral Blood CD8+ T Cells in Patients With Diffuse Large B-Cell Lymphoma.","authors":"Xi-Zhe Guo, Ya-Fei Guo, Shi-Xin Wu","doi":"10.1002/kjm2.70005","DOIUrl":"https://doi.org/10.1002/kjm2.70005","url":null,"abstract":"Diffuse large B-cell lymphoma (DLBCL) exhibits substantial biological and clinical heterogeneity. This study investigated the expression and prognostic implications of C-X-C chemokine receptor type 5 (CXCR5) and lymphocyte activation gene-3 (LAG-3) on peripheral blood CD8+ T cells in patients with DLBCL. A total of 71 DLBCL patients and 71 healthy controls were enrolled. The expression levels of CXCR5 and LAG-3 on peripheral blood CD8+ T cells were assessed and analyzed for their impact on 5-year progression-free survival (PFS) and overall survival (OS). Results revealed significantly elevated CXCR5 and LAG-3 expression levels in DLBCL patients compared to controls. CXCR5 expression correlated with lactate dehydrogenase (LDH) levels, extranodal involvement, Ann Arbor stage, and International Prognostic Index (IPI) scores, while LAG-3 expression was associated with Eastern Cooperative Oncology Group (ECOG) scores, number of extranodal sites, bone marrow involvement, Ann Arbor stage, and IPI scores. Multivariate analysis identified advanced age, Ann Arbor stage III-IV, and elevated CXCR5 and LAG-3 expression as independent risk factors for poorer 5-year PFS and OS. Furthermore, patients with higher CXCR5 and LAG-3 expression levels demonstrated significantly reduced 5-year PFS and OS rates. In conclusion, elevated CXCR5 and LAG-3 expression on peripheral blood CD8+ T cells plays a pivotal role in DLBCL progression and prognosis, making these markers potential therapeutic targets or prognostic indicators.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70005"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smoking and Elevated Preneoadjuvant Chemoradiotherapy Serum Carcinoembryonic Antigen Levels Are Associated With High Tumor Regression Grade and Poor Survival in Patients With Locally Advanced Rectal Cancer.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-13 DOI: 10.1002/kjm2.70008

Jen-Pin Chuang, Hsiang-Lin Tsai, Po-Jung Chen, Ching-Wen Huang, Wei-Chih Su, Tsung-Kun Chang, Yen-Cheng Chen, Ching-Chun Li, Yung-Sung Yeh, Jaw-Yuan Wang

{"title":"Smoking and Elevated Preneoadjuvant Chemoradiotherapy Serum Carcinoembryonic Antigen Levels Are Associated With High Tumor Regression Grade and Poor Survival in Patients With Locally Advanced Rectal Cancer.","authors":"Jen-Pin Chuang, Hsiang-Lin Tsai, Po-Jung Chen, Ching-Wen Huang, Wei-Chih Su, Tsung-Kun Chang, Yen-Cheng Chen, Ching-Chun Li, Yung-Sung Yeh, Jaw-Yuan Wang","doi":"10.1002/kjm2.70008","DOIUrl":"https://doi.org/10.1002/kjm2.70008","url":null,"abstract":"Neoadjuvant chemoradiotherapy (NACRT) is the standard treatment for patients with locally advanced rectal cancer (LARC). Tumor regression grade (TRG) is an essential prognostic factor in determining treatment efficacy. However, the potential factors influencing TRG in patients with rectal cancer who have received NACRT have not been investigated. We conducted a retrospective analysis of patients with LARC who received NACRT followed by surgical resection. We collected data on the patient characteristics, including age, sex, comorbidities, tumor size, lymph node status, time between NACRT and surgery, and pretreatment carcinoembryonic antigen (CEA) levels. TRG was determined on the basis of a pathological assessment of resected specimens, and overall survival (OS) at 5 years was determined. Univariate and multivariate logistic regression models were employed to evaluate the association between the patient characteristics and TRG. Univariate analysis revealed that smoking and prechemoradiotherapy (pre-CRT) and preoperative CEA levels were significantly associated with TRG. In a multivariate analysis, both smoking and higher pre-CRT CEA levels were identified as significant predictors of a high TRG. The hazard ratios were 2.32 (95% confidence interval [CI]: 1.06-5.07, p = 0.036) for smoking and 3.1 (95% CI: 1.69-5.68, p < 0.001) for higher pre-CRT CEA levels. In Kaplan-Meier analysis, the nonsmoker group exhibited higher OS (p = 0.004). Elevated pre-CRT CEA levels and current smoking status were associated with a more than two fold increase in the risk of a higher TRG after NACRT. Moreover, smoking was a significant risk factor for poor OS in patients with LARC following NACRT.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70008"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143618125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PSMD14 Transcriptionally Activated by MEF2A Promotes Pancreatic Cancer Development by Upregulating SPON2 Expression.

The Kaohsiung journal of medical sciences Pub Date : 2025-03-11 DOI: 10.1002/kjm2.70007

Yun-He Hao, Cheng-Ru Yang, Wu-Jiang Shi, Xiang-Yu Zhong

{"title":"PSMD14 Transcriptionally Activated by MEF2A Promotes Pancreatic Cancer Development by Upregulating SPON2 Expression.","authors":"Yun-He Hao, Cheng-Ru Yang, Wu-Jiang Shi, Xiang-Yu Zhong","doi":"10.1002/kjm2.70007","DOIUrl":"https://doi.org/10.1002/kjm2.70007","url":null,"abstract":"Proteasome 26S subunit non-ATPase 14 (PSMD14) plays a pro-carcinogenic role in various cancers. However, its specific effects and mechanisms in pancreatic cancer (PC) remain unclear. We aimed to assess the function and mechanism of PSMD14 in PC. Fifteen paired pancreatic ductal adenocarcinoma (PDAC) tissues and adjacent non-tumorous tissues were clinically obtained. Cell proliferation, migration, and invasion were assessed using colony formation, scratch, and Transwell assays. The interaction between the MEF2A transcription factor and the PSMD14 promoter verified by chromatin immunoprecipitation (ChIP) or dual luciferase assay. The interaction between RBM15B and SPON2 mRNA was validated by RNA immunoprecipitation (RIP) assay. The interaction between the proteins PSMD14 and RBM15B was detected by co-immunoprecipitation (Co-IP) assay. The m6A level of SPON2 was detected by methylated RNA immunoprecipitation (MeRIP, a common method for detecting m6A levels of mRNAs). The ubiquitination level of RNA-binding motif protein 15B (RBM15B) was detected using Co-IP. The role of PSMD14 in PC was further explored subcutaneous and lung metastasis models. PSMD14 was upregulated in PDAC tissues. PSMD14 knockdown inhibited PC cell viability, proliferation, migration, and invasion. MEF2A transcriptionally activated PSMD14 expression. PSMD14 knockdown promoted the ubiquitination degradation of RBM15B. Additionally, PSMD14 enhanced SPON2 mRNA stability through RBM15B-mediated m6A modification. SPON2 overexpression impaired the effect of knockdown PSMD14. Finally, PSMD14 knockdown in PC arrested tumor growth and lung metastasis. PSMD14, transcriptionally activated by MEF2A, promotes the de-ubiquitination of RBM15B, which upregulates SPON2 expression in an m6A-RBM15B-dependent manner, thereby facilitating PC proliferation, migration, and invasion.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70007"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0