The Kaohsiung journal of medical sciences最新文献_第2页

Preemptive Propofol Administration in Spinal Cord Injury: Effects on Pain-Induced Hypertension, Neuroinflammation, and Functional Recovery in Rats. 在脊髓损伤中预先注射丙泊酚：对疼痛引起的高血压、神经炎症和大鼠功能恢复的影响

The Kaohsiung journal of medical sciences Pub Date : 2025-04-01 DOI: 10.1002/kjm2.70011

Qun Cheng, Xiang-Yu Fang, Rong-En Qiu

{"title":"Preemptive Propofol Administration in Spinal Cord Injury: Effects on Pain-Induced Hypertension, Neuroinflammation, and Functional Recovery in Rats.","authors":"Qun Cheng, Xiang-Yu Fang, Rong-En Qiu","doi":"10.1002/kjm2.70011","DOIUrl":"https://doi.org/10.1002/kjm2.70011","url":null,"abstract":"Spinal cord injury (SCI) triggers secondary damage, including pain-induced hypertension, inflammation, and hemorrhage, impairing recovery. This study evaluated the efficacy of general anesthesia with preemptive propofol administration in mitigating secondary damage in SCI rats. SCI was induced in rats using a contusion model. Propofol (100 mg/kg) was administered intraperitoneally either 30 min before (preemptive) or 30 min after intermittent tail shock. Systolic blood pressure (SBP), body weight, food intake, inflammatory markers (interleukin-1 beta [IL-1β], interleukin-6 [IL-6]), hemorrhage markers, and serum levels of SCI biomarkers (glial fibrillary acidic protein [GFAP], myelin basic protein [MBP]) were measured. Functional recovery was assessed over 28 days using the Basso, Beattie, and Bresnahan (BBB) scale, horizontal ladder test, and rotarod test. Preemptive propofol administration effectively mitigated pain-induced hypertension, enhanced body weight and food intake, and reduced inflammatory markers to levels comparable to unstimulated SCI rats. In contrast, propofol administered post-stimulation was less effective. Preemptive administration significantly decreased GFAP levels and preserved MBP levels. Importantly, preemptive intervention reduced levels of hemoglobin and alpha hemoglobin, while post-stimulation intervention showed no significant effect on hemorrhage. Behavioral assessments demonstrated improved locomotor recovery, motor coordination, and balance in preemptively treated rats compared to delayed or no intervention. Preemptive administration of propofol effectively reduces pain-induced hypertension, inflammation, and gliosis while preserving myelin integrity and enhancing functional recovery in SCI rats. This intervention demonstrates significantly greater efficacy compared to delayed administration, underscoring the critical importance of timely treatment in mitigating secondary damage and improving outcomes after SCI.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70011"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143757091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Edematous changes and nail ulcers in hand transplant after SARS-COV-2 infection: Unraveling infection or rejection? SARS-COV-2感染手部移植术后的水肿变化和甲溃疡：是感染还是排斥？

The Kaohsiung journal of medical sciences Pub Date : 2025-04-01 Epub Date: 2025-01-15 DOI: 10.1002/kjm2.12941

Lee-Moay Lim, Yue-Chiu Su, Yur-Ren Kuo

引用次数: 0

Olfactory mucosa mesenchymal stem cell-derived exosomes protect against neuroinflammation after subarachnoid hemorrhage by activating mitophagy. 嗅觉粘膜间充质干细胞来源的外泌体通过激活线粒体自噬来保护蛛网膜下腔出血后的神经炎症。

The Kaohsiung journal of medical sciences Pub Date : 2025-04-01 Epub Date: 2025-02-19 DOI: 10.1002/kjm2.12951

Jian Wang, Jun Peng, Ling Gao, Jun He, Long Lin, Jia-Meng Li, Ying Xia

{"title":"Olfactory mucosa mesenchymal stem cell-derived exosomes protect against neuroinflammation after subarachnoid hemorrhage by activating mitophagy.","authors":"Jian Wang, Jun Peng, Ling Gao, Jun He, Long Lin, Jia-Meng Li, Ying Xia","doi":"10.1002/kjm2.12951","DOIUrl":"10.1002/kjm2.12951","url":null,"abstract":"Subarachnoid hemorrhage (SAH) can lead to significant acute neuroinflammation, with treatment outcomes often being inadequate. Olfactory mucosa mesenchymal stem cells (OM-MSCs) have promising therapeutic potential in nerve regeneration and functional recovery. This investigation sought to elucidate the functional mechanisms through which exosomes derived from OM-MSCs provide protection against neuroinflammation following SAH. Mouse OM-MSCs and their exosomes were isolated and characterized using various techniques, including transmission electron microscopy, immunofluorescence staining, Western blotting, flow cytometry, and nanoparticle tracking analysis. Hemin-induced HT22 cells were subsequently utilized to assess the impact of OM-MSC-derived exosomes on the inflammatory response, apoptosis, and mitophagy through ELISAs, Western blotting, qPCR, flow cytometry, and immunofluorescence staining. The impacts of exosomes on neuroinflammation and neuronal damage in SAH model mice were assessed using qPCR, ELISAs, Western blotting, immunofluorescence staining, and TUNEL staining. Exosomes derived from OM-MSCs had the capacity to reduce the levels of proinflammatory factors (IL-6, IL-1β, and TNF-α) and promote apoptosis in hemin-induced HT22 cells. Exosomes alleviated neuroinflammation and neuronal injury post-SAH, as evidenced by the increase in modified Garcia scores, reduction in the brain water content, decrease in blood-brain barrier permeability, decreases in inflammatory marker levels, and reduction in apoptosis rates. Notably, the protective effects of exosomes derived from OM-MSCs on neuroinflammation and apoptosis, both in vitro and in vivo, were mediated via the activation of mitophagy. These findings provide a fresh perspective for subsequent clinical research in the domain of prevention and treatment strategies.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e12951"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143451306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple Gastric Adenomyomas Mimicking Leiomyoma: A Novel Case Report. 多发性胃腺肌瘤模拟平滑肌瘤：一例新报告。

The Kaohsiung journal of medical sciences Pub Date : 2025-03-31 DOI: 10.1002/kjm2.70018

Chen Yuan, You-Hong Cao

引用次数: 0

Circulating let-7 Predicts Hepatic Fibrogenesis of 12-Month Post-Nucleos(t)ide Analog Treatment in Patients With Hepatitis B Virus. 循环let-7可预测乙型肝炎病毒感染者核苷酸类似物治疗后12个月的肝纤维化情况

The Kaohsiung journal of medical sciences Pub Date : 2025-03-29 DOI: 10.1002/kjm2.70015

Yi-Shan Tsai, Po-Cheng Liang, Yi-Hung Lin, Tyng-Yuan Jang, Yu-Ju Wei, Po-Han Chen, Jia-Ning Hsu, Meng-Hsuan Hsieh, Ming-Yen Hsieh, Chih-Wen Wang, Zu-Yau Lin, Ming-Lun Yeh, Chung-Feng Huang, Jee-Fu Huang, Ming-Lung Yu, Wan-Long Chuang, Chia-Yen Dai

{"title":"Circulating let-7 Predicts Hepatic Fibrogenesis of 12-Month Post-Nucleos(t)ide Analog Treatment in Patients With Hepatitis B Virus.","authors":"Yi-Shan Tsai, Po-Cheng Liang, Yi-Hung Lin, Tyng-Yuan Jang, Yu-Ju Wei, Po-Han Chen, Jia-Ning Hsu, Meng-Hsuan Hsieh, Ming-Yen Hsieh, Chih-Wen Wang, Zu-Yau Lin, Ming-Lun Yeh, Chung-Feng Huang, Jee-Fu Huang, Ming-Lung Yu, Wan-Long Chuang, Chia-Yen Dai","doi":"10.1002/kjm2.70015","DOIUrl":"https://doi.org/10.1002/kjm2.70015","url":null,"abstract":"Chronic hepatitis B virus (HBV) infection is associated with potential complications of liver cirrhosis and hepatocellular carcinoma. To date, there are no effective and noninvasive clinical markers that can predict the risk of liver fibrosis early and accurately in chronic hepatitis B (CHB) patients treated with nucleos(t)ide analogs (NAs). This study aimed to investigate the association of circulating let-7b/c/g levels with the severity of hepatic fibrosis with a FIB-4 index of 1.5-2.9 in CHB patients. We conducted a retrospective longitudinal study in patients with CHB after 6 months of NAs therapy to investigate whether serum let-7b/c/g levels can be monitored as an early biomarker for liver fibrogenesis based on multivariate logistic regression analyses. We also used the hepatic stellate cell line LX-2 treated with transforming growth factor-β (TGF-β) to evaluate the suppression effect of let-7b/c/g on hepatic fibrogenesis. The study showed that circulating let-7b/c/g could predict 12 months of antiviral treatment for HBV-related significant fibrosis (FIB-4 index ≥ 2.9) at baseline and was significantly negatively correlated with the FIB-4 score. Moreover, let-7b/c/g could directly target the TGF-βR1-3' untranslated region (3' UTR) and inhibit TGF-β induced p-SMAD2 phosphorylation to reduce α-smooth muscle actin levels, a fibrogenesis marker in LX-2 cells. These results confirm that let-7b/c/g could be a biomarker for monitoring HBV-induced fibrogenesis.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70015"},"PeriodicalIF":0.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of Metastasis in Small Pulmonary Oligonodules Detected in Breast Cancer Patients at Baseline CT. 基线CT对乳腺癌患者肺小结节转移的预测。

The Kaohsiung journal of medical sciences Pub Date : 2025-03-27 DOI: 10.1002/kjm2.70014

Huei-Yi Tsai, Min-Fang Chao, Tzu-Hsueh Tsai, Shen-Liang Shih, Fang-Ming Chen, Ming-Feng Hou, Ming-Yii Huang, Jui-Sheng Hsu

{"title":"Prediction of Metastasis in Small Pulmonary Oligonodules Detected in Breast Cancer Patients at Baseline CT.","authors":"Huei-Yi Tsai, Min-Fang Chao, Tzu-Hsueh Tsai, Shen-Liang Shih, Fang-Ming Chen, Ming-Feng Hou, Ming-Yii Huang, Jui-Sheng Hsu","doi":"10.1002/kjm2.70014","DOIUrl":"https://doi.org/10.1002/kjm2.70014","url":null,"abstract":"It is challenging for radiologists to diagnose pulmonary metastases when they encounter only a few (n ≤ 5) small pulmonary nodules (< 10 mm) on staging CT in breast cancer patients. We conducted this study to assess clinical and imaging features related to metastasis for better risk stratification. Retrospective analysis of 249 pulmonary nodules present at the baseline CTs of 194 patients diagnosed with breast cancer between 2014 and 2021 was performed. The evaluated features included nodular characteristics, perifissural nodules, associated imaging findings, clinical stage, and breast cancer subtype. Nodules with interval change were determined to be metastases. A large proportion of the patients had single nodule (78.9%) presence, and most of the nodules were less than 6 mm (86.3%). Among the 249 nodules, 63 (25.3%) nodules were in metastases. The independent predictors were nodule ≥ 6 mm, mediastinal/hilar lymphadenopathy, clinical Stages III and IV, and triple-negative breast cancer subtype. Nodules (≥ 6 mm) were assessed as weak evidence to rule in metastasis, and the results were as follows: positive likelihood ratio (+LR), 3.74; sensitivity, 30.2%; and specificity, 91.9%. With weak evidence of small pulmonary nodules (≧ 6 mm) to rule in metastases, it may be appropriate to follow the recommendations of growing nodule management. By contrast, the nodular shape, margin, location, perifissural nodules, and pleural tag did not show an association with metastasis.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70014"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adult Acute Megakaryoblastic Leukemia in a Mantle Cell Lymphoma Post Treatment and Auto-Transplant. 成人急性巨核母细胞白血病伴套细胞淋巴瘤治疗和自体移植。

The Kaohsiung journal of medical sciences Pub Date : 2025-03-26 DOI: 10.1002/kjm2.70013

Chin-Mu Hsu, Bi-Hua Du, Chien Hsiao, Hui-Hua Hsiao

引用次数: 0

Erythroderma Triggered by Cutaneous Fungal Infection and Prolonged Steroid Use: A Lesson Learned. 红皮病由皮肤真菌感染和长期使用类固醇引起：一个教训。

The Kaohsiung journal of medical sciences Pub Date : 2025-03-24 DOI: 10.1002/kjm2.70012

Chin-Yu Lee, Wei-Cheng Fang, Yue-Chiu Su, Yang-Yi Chen

引用次数: 0

EP300 Modulates MCM8 Transcription and Augments the Malignant Phenotype of Hepatitis B Virus-Positive Hepatocellular Carcinoma Cells. EP300调节MCM8转录并增强乙型肝炎病毒阳性肝癌细胞的恶性表型

The Kaohsiung journal of medical sciences Pub Date : 2025-03-17 DOI: 10.1002/kjm2.70006

Fang Xue, Tian-Feng Sun

{"title":"EP300 Modulates MCM8 Transcription and Augments the Malignant Phenotype of Hepatitis B Virus-Positive Hepatocellular Carcinoma Cells.","authors":"Fang Xue, Tian-Feng Sun","doi":"10.1002/kjm2.70006","DOIUrl":"https://doi.org/10.1002/kjm2.70006","url":null,"abstract":"Chronic infection with the hepatitis B virus (HBV) remains one of the primary drivers of the development of hepatocellular carcinoma (HCC), a highly aggressive malignancy with a grim prognosis. This study focused on the role of E1A-binding protein p300 (EP300) in the malignant phenotype of HBV-positive HCC cells and its functional mechanism. Increased EP300 expression was detected in HBV-positive tumor tissues and cells compared to their control counterparts. Silencing EP300 reduced tumorigenic activity, proliferation, viability, migration, invasion, and resistance to apoptosis of HBV-positive cells and reduced the concentrations of HBV infection markers HBsAg and HBeAg. These effects were achieved, at least in part, through downregulation of minichromosome maintenance 8 homologous recombination repair factor (MCM8). MCM8 was identified as a target of EP300 and mediated by acetylation modification. MCM8 was upregulated in HBV-positive tumors and HCC cells while decreasing following EP300 silencing in cells. However, the restoration of MCM8 expression in these cells rescued their malignant properties. In summary, this study suggests a role for EP300-mediated MCM8 upregulation in the malignant properties of HBV-positive HCC.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70006"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Clostridioides difficile Infection on Clinical Outcomes in Hospitalized IBD Patients and the Role of Fecal Microbiota Transplantation: A Retrospective Cohort Study. 艰难梭菌感染对IBD住院患者临床结局的影响及粪便菌群移植的作用：一项回顾性队列研究

The Kaohsiung journal of medical sciences Pub Date : 2025-03-17 DOI: 10.1002/kjm2.70002

Puo-Hsien Le, Chyi-Liang Chen, Chia-Jung Kuo, Pai-Jui Yeh, Chien-Chang Chen, Yi-Ching Chen, Cheng-Tang Chiu, Hao-Tsai Cheng, Yung-Kuan Tsou, Yu-Bin Pan, Cheng-Hsun Chiu

{"title":"Impact of Clostridioides difficile Infection on Clinical Outcomes in Hospitalized IBD Patients and the Role of Fecal Microbiota Transplantation: A Retrospective Cohort Study.","authors":"Puo-Hsien Le, Chyi-Liang Chen, Chia-Jung Kuo, Pai-Jui Yeh, Chien-Chang Chen, Yi-Ching Chen, Cheng-Tang Chiu, Hao-Tsai Cheng, Yung-Kuan Tsou, Yu-Bin Pan, Cheng-Hsun Chiu","doi":"10.1002/kjm2.70002","DOIUrl":"https://doi.org/10.1002/kjm2.70002","url":null,"abstract":"Clostridioides difficile infection (CDI) worsens the prognosis of patients with inflammatory bowel disease (IBD). This retrospective cohort study aimed to evaluate the risk factors, clinical manifestations, and outcomes of CDI in hospitalized patients with IBD, including those with toxin A/B results between April 2007 and April 2021. Patients were classified into the CDI and control groups. Patients with IBD and recurrent or refractory CDI underwent fecal microbiota transplantation (FMT). A total of 144 inpatients with IBD-45 in the CDI group and 99 in the control group-were analyzed. The incidence of CDI in inpatients with IBD was 31%. The Risk factors for CDI included longer IBD duration, biological therapy failure, and biological use. More patients in the CDI group presented with abdominal pain (77.8% vs. 55.6%, p = 0.011). In the antibiotic treatment-only group, the symptom improvement rate was 60.7% (17/28), the microbiological cure rate was 89.3% (25/28), and the overall success rate was 71.4% (20/28). After antibiotic treatment and FMT, 71.4% (10/14) of the patients tested negative for CDI, and 64.3% (9/14) had improved clinical symptoms. CDI led to more hospitalizations (median two times [range 0-12] vs. median one time [range 0-19], p = 0.008), a lower steroid-free remission rate (46.7% vs. 67.7%, p = 0.017), and higher Mayo scores (median 5 points [range 2-12] vs. median 3 points [range 0-12]). Patients who received FMT had fewer hospitalizations and fewer IBD-related complications during follow-up than those who received antibiotics alone. FMT should be considered in patients with IBD with refractory or recurrent CDI to improve clinical outcomes.","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"e70002"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0