富血小板血浆和富血小板纤维蛋白对双种生物膜烧伤创面愈合的影响。

Wen-Dan Li, Fang Lin, Yu Sun, Zi-Jing Zhu, Mei-Liang Luo, Yi-Qi Zeng, Zhen Lin, Mou Zhou
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引用次数: 0

摘要

本研究评价了富血小板血浆(PRP)和富血小板纤维蛋白(PRF)对双种生物膜烧伤创面的影响。采用铜绿假单胞菌(P. aeruginosa)和金黄色葡萄球菌(S. aureus)感染大鼠烧伤创面,建立双种生物膜模型。感染后用离子银(AG)、PRF和PRP处理伤口。用银扫描电镜(SEM)观察感染后的粘附情况。PRF和PRP从烧伤后第8天开始减少创面大小,而AG在烧伤后第12天显著促进创面愈合。与AG和对照组相比,PRF组和PRP组新胶原蛋白的形成时间最短。PRF和PRP可显著降低金黄色葡萄球菌和铜绿假单胞菌生物膜创面细菌数量,而AG抑菌效果较弱。AG、PRF和PRP治疗显著减少炎症介质和诱导VEGFA。然而,AG处理使TNF-α升高。PRF和PRP能促进双种生物膜感染下的创面愈合,并对金黄色葡萄球菌和铜绿假单胞菌具有较强的抗菌活性,提示PRF和PRP可能是治疗双种生物膜感染烧伤创面的潜在药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of platelet-rich plasma and platelet-rich fibrin on healing of burn wound with dual-species biofilm.

This study evaluated the impact of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) on burn wound with dual-species biofilm. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) were applied to infect the burn wound in rats to establish a dual-species biofilm model. After infection, the wound was treated with ionized silver (AG), PRF, and PRP. Silver scanning electron microscopy (SEM) was used to assess adhesion after infection. PRF and PRP reduced wound size from day 8 after burn injuries, while AG significantly promoted burn wound healing at day 12. New collagen was formed in the shortest time in PRF and PRP groups compared to AG and control groups. PRF and PRP greatly lowered the bacterial numbers in wounds with S. aureus and P. aeruginosa biofilm, whereas AG showed weak bacteriostatic effects. AG, PRF, and PRP treatments significantly reduced inflammatory mediators and induced VEGFA. However, AG treatment increased TNF-α. PRF and PRP accelerate wound healing in the presence of dual-species biofilm infection and show strong antibacterial activity against S. aureus and P. aeruginosa, indicating that PRF and PRP could be potential therapies for burn wounds with dual-species biofilm infection.

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