The International journal of developmental biology最新文献

{"title":"Unraveling hepatic consequences of intrauterine growth restriction and catch-up growth: insights from histological, biochemical and metabolomic analysis in rats.","authors":"Mukaddes Esrefoğlu, Sahabettin Selek, Fatmanur Koktasoglu, Nihan Bayindir, Emine-Rumeysa Hekimoglu, Seda Kirmizikan, Fatma-Bedia Karakaya-Cimen, Halime Dulun-Agac, Mehtap Alim, Birsen Elibol, Ozge Pasin, Somer Bekiroglu","doi":"10.1387/ijdb.240147me","DOIUrl":"10.1387/ijdb.240147me","url":null,"abstract":"<p><p>Intrauterine growth restriction (IUGR) is increasingly recognized as a significant risk factor for metabolic disorders in adulthood. Employing a multi-faceted approach encompassing histopathological, immunohistochemical, biochemical, Western-blotting, and metabolomics analyses, this study aimed to elucidate potential metabolite markers of IUGR, and catch-up growth-related metabolic disturbances and the underlying metabolic pathways implicated in IUGR pathogenesis. This study cohort comprised 54 male siblings from 20 Sprague-Dawley female young rats. On the 19th day of gestation, half of the pregnant rats underwent bilateral uterine artery ligation, while the remaining half underwent a simulated surgical intervention involving solely peritoneal incisions. Blood and liver samples were collected from the pups after attaining catch-up growth at the postnatal weeks 2, 4, and 8. IUGR rats exhibited a spectrum of changes including histological abnormalities, altered apoptosis rates, oxidative stress markers, and mitochondrial energy metabolism. Metabolomic analysis revealed dysregulation in multiple metabolic pathways encompassing galactose, propanoate, glycerolipid, cysteine, methionine, and tyrosine metabolism, among others. Notably, disturbances were observed in butanoate, glutathione metabolism, valine, leucine, and isoleucine biosynthesis and degradation, citrate cycle, aminoacyl-tRNA biosynthesis, as well as glycolysis/gluconeogenesis. Our metabolomics analysis provides insights into the potential disease susceptibility of individuals born with IUGR, including obesity, diabetes, heart failure, cancer, mental retardation, kidney and liver diseases, and cataracts. These findings underscore the intricate interplay between intrauterine conditions and long-term metabolic health outcomes, highlighting the need for further investigation into preventive and therapeutic strategies aimed at mitigating the risk of metabolic diseases in individuals with a history of IUGR.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"35-50"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental transcriptome reveals the placental brain axis genes and pathways of gestational diabetes mellitus (GDM) affecting offspring neurodevelopment.","authors":"Jianhua Li, Qian Liu, Xuhui Liu, Yunyun Wang, Yuxia Jin, Weikai Wang, Bin Yi, Yanxia Wang","doi":"10.1387/ijdb.240170jl","DOIUrl":"10.1387/ijdb.240170jl","url":null,"abstract":"<p><p>This study aims to analyze the pathways and the placental brain axis genes of gestational <i>diabetes mellitus</i> (GDM) affecting offspring neurodevelopment. Differentially expressed genes (DEGs) were identified through transcriptome sequencing of placental tissues. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed on DEGs. A protein-protein interaction (PPI) network was constructed and annotated using the STRING online software. The expression of neurodevelopment-related genes was analyzed by qPCR. Hubgenes were analyzed using Cytoscape 3.7.1 software. The correlation between Hubgenes and placental brain axis genes was analyzed through literatures alignment. The pathways of GDM affecting offspring neural development were predicted using the KEGG database. The placental transcriptome revealed that there were 404 DEGs between GDM and Normal groups. Among these DEGs, 125 were upregulated and 279 were downregulated. GO analysis indicated that DEGs were mainly involved in intracellular calcium activated chloride channel activity, anion channel activity, G protein-coupled peptide receptors, etc. Additionally, KEGG analysis revealed that DEGs were predominantly involved in neuroactive ligand receptor interaction pathways. STRING online software analysis revealed that the DLGAP1, NXNL2, SCG2, SLC18A2, LYNX1, GRM1, DLGAP1, BIRC7 genes were associated with neurodevelopment. PCR validation of these 8 genes was consistent with transcriptome results (<i>P</i><0.05). Literatures alignment showed that DLGAP1, GRM1 and SLC18A2 are placental brain axis genes that influence offspring neurodevelopment. The placental brain axis genes DLGAP1, GRM1, SLC18A2 have been found to influence GDM offspring neurodevelopment through the regulation of the Gq/PLC/PKC pathway.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 1","pages":"51-59"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sialylated glycoproteins and sialyltransferases involved in mesoderm-derived organ formation during embryogenesis.","authors":"Ingrid Fliniaux, Pélagie Douchez, Yoshiko Takeda-Uchimura, Marin Matthieu, Alain Martoriati, Nao Yamakawa, Caroline Molinaro, Jean François Bodart, Anne Harduin-Lepers, Katia Cailliau","doi":"10.1387/ijdb.250129km","DOIUrl":"10.1387/ijdb.250129km","url":null,"abstract":"<p><p>Most major organs, like muscles, bones, vessels and kidneys, develop from the mesoderm, one of three germ cell layers in triploblastic organisms. Sialic acids significantly affect embryonic development by regulating cell division, migration and death through signaling pathways and cell adhesion, which support morphogenesis. Loss of early biosynthetic enzymes reduces embryonic viability and leads to complex phenotypes, while the loss of terminal enzymes primarily results in tissue-specific defects in mesoderm-derived organs. Key sialylated glycoproteins involved in the developmental processes of mesoderm and mesoderm-derived organs have been identified across various species as major effectors. These enzymes and glycoproteins are of significant interest and are discussed in the present review.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 4","pages":"161-172"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynein axonemal assembly factors (<i>dnaaf</i>) 5 and 9 are expressed in ciliated organs of zebrafish embryos.","authors":"Usharani Nayak, Kalyani Sahoo, Rajeeb K Swain","doi":"10.1387/ijdb.250162rs","DOIUrl":"10.1387/ijdb.250162rs","url":null,"abstract":"<p><p>Dynein axonemal assembly factors (DNAAFs) play crucial roles in the formation and function of motile cilia, and their dysfunction often results in primary ciliary dyskinesia (PCD). We report the spatio-temporal expression patterns of <i>dnaaf5</i> and <i>dnaaf9</i> mRNA in zebrafish embryos, providing insight into their possible functions during development. We show that <i>dnaaf5</i> and <i>dnaaf9</i> mRNAs are expressed in motile ciliated tissues, such as the Kupffer's vesicle, pronephros, floor plate, brain and olfactory placode. The <i>dnaaf5</i> and <i>dnaaf9</i> crispants develop ciliopathic defects during zebrafish development. These data suggest that <i>dnaaf5</i> and <i>dnaaf9</i> may regulate motile cilia biogenesis and function in zebrafish. Our findings suggest functional redundancy and divergence among dynein arm assembly factors in vertebrates.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 4","pages":"195-202"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chloroquine sensitivity gradient induces tissue regeneration and maintenance phenotypes in planarians.","authors":"Rufus James, Justin M Tavalsky, Michael C Tyree, Robert J Major","doi":"10.1387/ijdb.250101rm","DOIUrl":"10.1387/ijdb.250101rm","url":null,"abstract":"<p><p>Failure to properly regenerate and maintain tissues is a root cause of disease and aging. One goal of regenerative biology is to unveil mechanisms by which simpler model organisms successfully regenerate so that we can better understand how to control the cellular and molecular switches in human tissues. Planarians have become a favored model system for these studies because of their amazing capacity to fully and rapidly regenerate following amputation, but also for their response to other stressors such as starvation. Planarians undergo proportional degrowth when starved, a unique process that balances stem cell proliferation and apoptotic cell death, autophagy-mediated cell turnover, and energy sensing. We chose a pharmacological approach to study autophagy in planarians by using chloroquine, an autophagy inhibitor. We found that head regression and tissue lysis were induced by chloroquine in whole planarians and that starved planarians were more sensitive than their fed counterparts. While chloroquine treatment did not prevent tissue regeneration following injury, it caused regeneration delays and disruptions in photoreceptor replacement. Finally, we show that an anterior-posterior gradient of chloroquine sensitivity exists in the planarian with prepharyngeal tissues exhibiting significantly low sensitivity. Together, our results extend our understanding of autophagic flux in planarian tissue regeneration and maintenance and establish a model by which an autophagic gradient exists in this highly regenerative system.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"69 4","pages":"187-194"},"PeriodicalIF":1.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147793755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Genetic Odyssey of Axolotl Regeneration: Insights and Innovations.","authors":"Muhammad Faisal, Afshan Mehreen, Deli Hays, Faiza Yaseen, Yujun Liang","doi":"10.1387/ijdb.240111yl","DOIUrl":"10.1387/ijdb.240111yl","url":null,"abstract":"<p><p>The axolotl, a legendary creature with the potential to regenerate complex body parts, is positioned as a powerful model organism due to its extraordinary regenerative capabilities. Axolotl can undergo successful regeneration of multiple structures, providing us with the opportunity to understand the factors that exhibit altered activity between regenerative and non-regenerative animals. This comprehensive review will explore the mysteries of axolotl regeneration, from the initial cellular triggers to the intricate signaling cascades that guide this complex process. We will delve deeply into the multifaceted interplay of genes and factors, highlighting the key role of signaling pathways and the influence of epigenetic modifications (such as DNA methylation, histone modification, and miRNA regulation) during regeneration. Furthermore, we will discuss how axolotls defy the odds by showing remarkable resistance to cancer, offering insights into potential therapeutic strategies. However, that is not the end; we will also highlight how age might affect the regenerative power of this creature. We hope this review will help navigate the awe-inspiring realm of axolotl regeneration, advance our understanding of regenerative biology, and chart pathways for future investigations aimed at uncovering new therapeutic approaches.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"68 3","pages":"103-116"},"PeriodicalIF":1.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast Growth Factor 8 enhances the chondrogenesis of trunk neural crest cells: a possible gene regulatory network.","authors":"Raphaella Josino, Saloe Bispo, Bernardo Bonilauri, Bruno Dallagiovanna, Giordano Wosgrau Calloni","doi":"10.1387/ijdb.240189gc","DOIUrl":"10.1387/ijdb.240189gc","url":null,"abstract":"<p><p>The neural crest (NC) is an embryonic cell population with high migratory capacity. It contributes to forming several organs and tissues, such as the craniofacial skeleton and the peripheral nervous system of vertebrates. Both pre-migratory and post-migratory NC cells are plastic, adopting multiple differentiation paths by responding to different inductive environmental signals. Cephalic neural crest cells (CNCCs) give rise to most of the cartilage and bone tissues in the head. On the other hand, the mesenchymal potential of trunk neural crest cells (TNCCs) is sparsely detected in some animal groups. The mesenchymal potential of TNCCs can be unveiled through specific environmental conditions of NC cultures. In this study, we present evidence that FGF8 treatment can foster increased chondrogenic differentiation of TNCCs, particularly during treatment at the migratory stage. Additionally, we conducted a transcriptomic analysis of TNCCs in the post-migratory stage, noting that exogenous FGF8 signaling can sustain multipotent status and, possibly, at the same time, a pro-cartilage regulatory gene network. Our results provide a more comprehensive understanding of the mechanisms underlying chondrogenic differentiation from TNCCs.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"68 3","pages":"135-143"},"PeriodicalIF":1.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell number regulation occurs during the pre-gastrulation period of postimplantation development in double chimeric mouse embryos.","authors":"Katarzyna Krawczyk, Magdalena Oślislok, Anna Gałązkiewicz, Marcin Szpila, Marek Maleszewski","doi":"10.1387/ijdb.240138mm","DOIUrl":"10.1387/ijdb.240138mm","url":null,"abstract":"<p><p>Aggregates of two mouse embryos produce viable offspring of normal size, indicating that there are mechanisms in the embryo that can downregulate their size to the size of the corresponding normal (single) embryos. Very little is known about the mechanisms controlling compensation for increased preimplantation size. Also, it is still elusive when exactly during development chimeric embryos regulate their size. Here, we determined the exact period of size regulation in chimeras. Using a chimeric embryo produced by aggregating two 8-cell stage embryos, we revealed that size regulation initiates shortly after implantation (E5.5) and ends with the start of gastrulation (E7.5). Importantly, processes that regulate cell number in chimeric embryos do not disturb morphogenesis, so that the formation of the proamniotic cavity occurs in parallel with size regulation.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"68 3","pages":"127-133"},"PeriodicalIF":1.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological characterisation of the horn bud region in 58 day old bovine fetuses.","authors":"Johanna E Aldersey, Tong Chen, Kiro Petrovski, John L Williams, Cynthia D K Bottema","doi":"10.1387/ijdb.240040ja","DOIUrl":"10.1387/ijdb.240040ja","url":null,"abstract":"<p><p>The presence of horns in domestic ruminants, such as cattle, sheep and goats, has financial and welfare implications. The genetic interactions that lead to horn development are not known. Hornless, or polled, cattle occur naturally. The known causative DNA variants (Celtic, Friesian, Mongolian and Guarani) are in intergenic regions on bovine chromosome 1, but their functions are not known. It is thought that horns may be derived from cranial neural crest stem cells and the POLLED variants disrupt the migration or proliferation of these cells. Relaxin family peptide receptor 2 (<i>RXFP2</i>) is more highly expressed in developing horns in cattle compared to nearby skin and has been shown to play a role in horn development in sheep. However, the role of RXFP2 in horn formation is not understood. Histological analyses of cranial tissues from homozygous horned and polled cattle fetuses at day 58 of development was carried out to determine the differences in the structure of the horn bud region. Condensed cells were only observed in the horn bud mesenchyme of horned fetuses and could be the progenitor horn cells. The distribution of neural crest markers (SOX10 and NGFR) and RXFP2 between horned and polled tissues by immunohistochemistry was also analysed. However, SOX10 and NGFR were not detected in the condensed cells, and therefore, these cells are either not derived from the neural crest, or have differentiated and no longer express neural crest markers. SOX10 and NGFR were detected in the peripheral nerves, while RXFP2 was detected in peripheral nerves and in the horn bud epidermis. Previous research has shown that RXFP2 variants are associated with horn phenotypes in cattle an sheep. Therefore, the RXFP2 variants may affect the development of the epidermis or peripheral nerves in the horn bud.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"117-126"},"PeriodicalIF":1.3,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Origin and Development of Interstitial Cells of Cajal.","authors":"Tara Sweet, Christeen M Abraham, Adam Rich","doi":"10.1387/ijdb.240057ar","DOIUrl":"10.1387/ijdb.240057ar","url":null,"abstract":"<p><p>The digestive tract is a series of organs with specific functions and specialized anatomy. Each organ is organized similarly with concentric layers of epithelial, connective, smooth muscle, and neural tissues. Interstitial cells of Cajal (ICC) are distributed in smooth muscle layers and contribute to the organization of repetitive and rhythmic smooth muscle contractions. Understanding ICC development is critical to understanding gastrointestinal motility patterns. Experiments determining ICC origin and development in mice, chicken, and humans are described, as well as what is known in the zebrafish. At least six types of ICC in the digestive tract have been described and ICC heterogeneity in adult tissues is reviewed. Factors required for ICC development and for maintenance of ICC subclasses are described. This review is suitable for those new to ICC development and physiology, especially those focused on using zebrafish and other model systems.</p>","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":" ","pages":"93-102"},"PeriodicalIF":1.3,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}