小鼠淋巴内皮细胞的基因靶向:当前策略与未来展望。

Hans Schoofs, Taija Mäkinen
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引用次数: 0

摘要

不同器官内的淋巴管具有不同的发育起源,其发育和维持依赖于不同的生长因子信号通路,并显示出明显的组织特异性适应,这有助于它们在正常生理和各种疾病中发挥作用。淋巴管的功能研究广泛依赖于小鼠模型的使用,这种模型允许选择性基因靶向淋巴管内皮细胞(LEC)。在此,我们将讨论淋巴管内皮细胞的多样性,并概述一些常用的淋巴管内皮细胞特异性诱导 Cre 株系和诱导方案,概述基本的实验参数及其影响。我们介绍了针对胚胎、出生后和成年 LEC 的优化治疗方案,有效地针对淋巴管研究中常用的器官,如肠系膜和皮肤。我们进一步强调了与每种诱导方案和小鼠品系相关的预期结果和局限性。建议的方案可作为实验室启动淋巴管靶向研究的推荐方案,旨在促进淋巴管领域的品系追踪和功能研究的统一性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic targeting of lymphatic endothelial cells in mice: current strategies and future perspectives.

Lymphatic vessels within different organs have diverse developmental origins, depend on different growth factor signaling pathways for their development and maintenance, and display notable tissue-specific adaptations that contribute to their roles in normal physiology and in various diseases. Functional studies on the lymphatic vasculature rely extensively on the use of mouse models that allow selective gene targeting of lymphatic endothelial cells (LECs). Here, we discuss LEC diversity and provide an overview of some of the commonly used LEC-specific inducible Cre lines and induction protocols, outlining essential experimental parameters and their implications. We describe optimized treatment regimens for embryonic, postnatal and adult LECs, efficiently targeting organs that are commonly studied in lymphatic vascular research, such as the mesentery and skin. We further highlight the anticipated outcomes and limitations associated with each induction scheme and mouse line. The proposed protocols serve as recommendations for laboratories initiating studies involving targeting of the lymphatic vasculature, and aim to promote uniformity in lineage tracing and functional studies within the lymphatic vascular field.

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