Prostaglandins, leukotrienes, and essential fatty acids最新文献

{"title":"Subgroup analyses of a randomized trial of DHA supplementation for infants born preterm with assessments of cognitive development up to 7-years of age: What happens in infants born <29 weeks’ gestation?","authors":"JF Gould ,&nbsp;JM Bednarz ,&nbsp;TR Sullivan ,&nbsp;AJ McPhee ,&nbsp;RA Gibson ,&nbsp;M Makrides","doi":"10.1016/j.plefa.2023.102593","DOIUrl":"https://doi.org/10.1016/j.plefa.2023.102593","url":null,"abstract":"<div><p>A recent trial showed that high-dose docosahexaenoic acid (high-DHA) supplementation of infants born &lt;29 weeks’ gestation improves intelligence quotient (IQ) at five years’ corrected age. However, this finding has not been detected by other trials of DHA, which either did not measure IQ or included more mature infants. We analyzed the subgroup of 204 infants born &lt;29 weeks’ from our earlier randomized trial of high-DHA (∼1 % total fatty acids) or standard-DHA (∼ 0.3 % total fatty acids). Participants were assessed for cognition at 18 months, and IQ and behavior at seven years’ corrected age. No group differences were detected for mean cognitive, IQ or behavior scores. At 18 months, 18.8 % of children in the high-DHA group had a cognitive score &lt;85, compared with 31.1 % of children in the standard-DHA group, but at seven years there was no difference. Although an underpowered post-hoc subgroup analysis, this study provides limited support to recommendations that infants born &lt;29 weeks’ gestation require supplemental DHA.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"198 ","pages":"Article 102593"},"PeriodicalIF":3.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134688937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phospholipase D1 activity is crucial for cytosolic phospholipase A2 –dependent prostaglandin E2 formation in murine osteoblastic MC3T3-E1 cells","authors":"Hans Jörg Leis ,&nbsp;Werner Windischhofer","doi":"10.1016/j.plefa.2023.102592","DOIUrl":"https://doi.org/10.1016/j.plefa.2023.102592","url":null,"abstract":"<div><p>In bone, prostaglandin E₂ (PGE₂) is highly osteogenic and formed by osteoblasts, a key modulatory event in the regulation of bone cell activity. MC3T3-E1 cells are widely used as an in vitro model of osteoblast function. It is still not clear which pathways contribute to the release of AA in these cells. In this study we have focussed on the contribution of phospholipase D (PLD) enzymes to osteoblastic PGE₂ formation after stimulation with endothelin-1 (ET-1). Using specific inhibitors of PLD1 and PLD2 we could show that PGE₂ formation was strictly dependent on PLD1 but not PLD2 activity and cytosolic phospholipase A₂ (cPLA₂) was activated by triggering through PLD1. We have identified diacyl glycerol (DAG) as a possible effector molecule which may serve as a triggering signal for PKC activation and subsequent cPLA₂ phosphorylation.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"198 ","pages":"Article 102592"},"PeriodicalIF":3.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0952327823000613/pdfft?md5=782794284ac1a31b14b69afcd1bc5318&pid=1-s2.0-S0952327823000613-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92060844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of maternal fish oil supplementation during lactation on socioemotional wellbeing and physical activity in 13-year-old children: A randomized clinical trial","authors":"Lotte Lauritzen ,&nbsp;Emilie R. Hegelund ,&nbsp;Sara E. Eriksen ,&nbsp;Janni Niclasen ,&nbsp;Kim F. Michaelsen","doi":"10.1016/j.plefa.2023.102588","DOIUrl":"10.1016/j.plefa.2023.102588","url":null,"abstract":"<div><p>Early dietary long-chain n-3PUFA (n-3LCPUFA) may affect brain development. We investigated if fish oil supplementation of lactating mothers affected socioemotional wellbeing in adolescents in a potentially gender-specific manner. At age 13, we invited 92 children of mothers who completed a randomized trial with 1.5 g/d n-3 LCPUFA or olive oil during the first 4 months of lactation and 48 children of mothers with a high habitual fish intake. Children and parents answered validated questionnaires regarding socioemotional wellbeing and physical activity was monitored by ActiGraph for 7 days. Participation rate was 71%. Univariate correlations between children's and parents’ ratings on the individual scales were moderate-strong, but correlations across questionnaires indicated that parents might base their ratings on proxy markers. We found no group differences in self-rated socioemotional outcomes or physical activity. Although the study was small, it was the first follow-up on effects of perinatal n-3LCPUFA supply on socioemotional wellbeing in adolescence.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"197 ","pages":"Article 102588"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10561292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"15-lipoxygenase and cyclooxygenase expression profile and their related modulators in COVID-19 infection","authors":"Sara Kianfar ,&nbsp;Vahid Salimi ,&nbsp;Alireza Jahangirifard ,&nbsp;Seyed Bashir Mirtajani ,&nbsp;Mohammad Amin Vaezi ,&nbsp;Jila Yavarian ,&nbsp;Talat Mokhtari-Azad ,&nbsp;Masoumeh Tavakoli-Yaraki","doi":"10.1016/j.plefa.2023.102587","DOIUrl":"10.1016/j.plefa.2023.102587","url":null,"abstract":"<div><h3>Background</h3><p>The role of the lipoxygenase (LOX) and cyclooxygenase (COX) enzymes in maintaining cellular homeostasis and regulating immune responses promoted us in this study to analyze the pattern of changes in 15-lipoxygenase and cyclooxygenase isoforms and their related cytokines in SARS-CoV-2 infection.</p></div><div><h3>Methods</h3><p>15-LOX-1, 15-LOX-2, COX-1 and COX-2 gene expression levels were determined using qRT-PCR in nasopharynx specimens from patients with severe [<em>N</em> = 40] and non-severe [<em>N</em> = 40] confirmed SARS-CoV-2 infections and healthy controls. Circulating levels of lL-6, lL-10, PGE2, and IFN-γ were measured in patients and healthy controls using ELISA assay. The associations between the measured variables and the patient's clinic-pathological characteristics were assessed for all groups.</p></div><div><h3>Results</h3><p>The expression level of 15-LOX-1 was elevated significantly in male patients with severe infection; although female patients showed a different expression profile. 15-LOX-2 expression level was considerably increased in male patients with severe infection; while changes in its expression remained inconclusive in female patients. The relationship between 15-LOX expression and the male gender was prominent. Both COX isoforms expression showed elevation in male and female patients that were correlated with disease severity. The simultaneous increase in lL-6, PGE2 and IFN-γ levels also decrease in lL-10 in patients with severe infection indicating the possible regulatory network related to the COX and 15-LOX enzymes in the output of the SARS-CoV-2 infection.</p></div><div><h3>Conclusion</h3><p>The results of this study determined the pattern of possible changes in key enzymes of prostaglandin and eicosanoids synthesis pathway and their mediators, which can be helpful in mapping the SARS-CoV-2 pathogenicity and pharmaceutical approaches.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"197 ","pages":"Article 102587"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10618332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolvin E1 heals injured cardiomyocytes: Therapeutic implications and H-FABP as a readout for cardiovascular disease & systemic inflammation","authors":"A. Zheng ,&nbsp;N. Huang ,&nbsp;D. Bean ,&nbsp;S. Rayapaneni ,&nbsp;Jude Deeney ,&nbsp;M. Sagar ,&nbsp;James A. Hamilton","doi":"10.1016/j.plefa.2023.102586","DOIUrl":"10.1016/j.plefa.2023.102586","url":null,"abstract":"<div><p>The purpose of this study is to investigate heart-fatty acid binding protein (H-FABP) leakage from cardiomyocytes as a quantitative measure of cell membrane damage and to test healing by Resolvin E1 (RVE1) as a potential therapeutic for patients with inflammatory diseases (cardiovascular disease and comorbidities) with high morbidity and mortality. Our quantitative ELISA assays demonstrated H-FABP as a sensitive and reliable biomarker for measuring cardiomyocyte damage induced by lipopolysaccharide (LPS) and healing by RvE1, a specialized pro-resolving mediator (SPM) derived from the Omega-3 fatty acid, eicosapentaenoic acid (EPA), a dietary nutrient that balances inflammation to restore homeostasis. RvE1 reduced leakage of H-FABP by up to 86%, which supports our hypothesis that inflammation as a mechanism of injury can be targeted for therapy. H-FABP as a blood biomarker was tested in 40 patients admitted to Boston Medical Center for respiratory distress, (20 patients with and 20 patients without COVID infection). High levels of H-FABP correlated with clinically diagnosed CVD, diabetes, and end-stage renal disease (ESRD) in both patient groups. The level of H-FABP indicates not only CVD damage but is a valuable measure for patients with increased inflammation disease comorbidities.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"197 ","pages":"Article 102586"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10041537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased plasma levels of sphingolipids and total cholesterol in adult cystic fibrosis patients","authors":"Dilara Bal Topcu ,&nbsp;Berrin Er ,&nbsp;Filiz Ozcan ,&nbsp;Mutay Aslan ,&nbsp;Lutfi Coplu ,&nbsp;Incilay Lay ,&nbsp;Yesim Oztas","doi":"10.1016/j.plefa.2023.102590","DOIUrl":"10.1016/j.plefa.2023.102590","url":null,"abstract":"<div><h3>Background</h3><p>Sphingolipid species in the lung epithelium have a critical role for continuity of membrane structure, vesicular transport, and cell survival. Sphingolipid species were reported to have a role in the inflammatory etiology of cystic fibrosis by previous work. The aim of the study was to investigate the levels of plasma sphingomyelin and ceramide in adult cystic fibrosis (CF) patients and compared with healthy controls.</p></div><div><h3>Materials and methods</h3><p>Blood samples were obtained from CF patients at exacerbation (<em>n</em> = 15), discharge (<em>n</em> = 13) and stable periods (<em>n</em> = 11). Healthy individuals (<em>n</em> = 15) of similar age served as control. Levels of C16–C24 sphingomyelin and C16–C24 ceramide were measured in the plasma by LC-MS/MS. Also, cholesterol and triglyceride levels were determined in plasma samples of the patients at stable period.</p></div><div><h3>Results</h3><p>All measured sphingomyelin and ceramide levels in all periods of CF patients were significantly lower than healthy controls except C16 sphingomyelin level in the stable period. However, plasma Cer and SM levels among exacerbation, discharge, and stable periods of CF were not different. CF patients had significantly lower cholesterol levels compared to healthy individuals. We found significant correlation of cholesterol with C16 sphingomyelin.</p></div><div><h3>Conclusion</h3><p>We observed lower plasma Cer and SM levels in adult CF patients at exacerbation, discharge, and stable periods compared to healthy controls. We didn't find any significant difference between patient Cer and SM levels among these three periods. Our limited number of patients might have resulted with this statistical insignificance. However, percentage of SM16 levels were increased at discharge compared to exacerbation levels, while percentage of Cer16 and Cer 20 decreased at stable compared to exacerbation. Inclusion of a larger number of CF patients in such a follow up study may better demonstrate any possible difference between exacerbation, discharge, and stable periods.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"197 ","pages":"Article 102590"},"PeriodicalIF":3.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41159127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor microenvironment-derived monoacylglycerol lipase provokes tumor-specific immune responses and lipid profiles","authors":"Eva Gruden ,&nbsp;Melanie Kienzl ,&nbsp;Carina Hasenoehrl ,&nbsp;Arailym Sarsembayeva ,&nbsp;Dusica Ristic ,&nbsp;Sophie Theresa Schmid ,&nbsp;Kathrin Maitz ,&nbsp;Ulrike Taschler ,&nbsp;Lisa Hahnefeld ,&nbsp;Robert Gurke ,&nbsp;Dominique Thomas ,&nbsp;Julia Kargl ,&nbsp;Rudolf Schicho","doi":"10.1016/j.plefa.2023.102585","DOIUrl":"10.1016/j.plefa.2023.102585","url":null,"abstract":"<div><p>We recently described that monoacylglycerol lipase (MGL) is present in the tumor microenvironment (TME), increasing tumor growth. In this study we compare the implications of MGL deficiency in the TME in different tumor types.</p><p>We show that subcutaneous injection of KP (Kras^LSL-G12D/p53^fl/fl, mouse lung adenocarcinoma) or B16-F10 cells (mouse melanoma) induced tumor growth in MGL wild type (WT) and knockout (KO) mice. MGL deficiency in the TME attenuated the growth of KP cell tumors whereas tumors from B16-F10 cells increased in size. Opposite immune cell profiles were detected between the two tumor types in MGL KO mice. In line with their anti-tumorigenic function, the number of CD8⁺ effector T cells and eosinophils increased in KP cell tumors of MGL KO vs. WT mice whereas their presence was reduced in B16-F10 cell tumors of MGL KO mice. Differences were seen in lipid profiles between the investigated tumor types. 2-arachidonoylglycerol (2-AG) content significantly increased in KP, but not B16-F10 cell tumors of MGL KO vs. WT mice while other endocannabinoid-related lipids remained unchanged. However, profiles of phospho- and lysophospholipids, sphingomyelins and fatty acids in KP cell tumors were clearly distinct to those measured in B16-F10 cell tumors.</p><p>Our data indicate that TME-localized MGL impacts tumor growth, as well as levels of 2-AG and other lipids in a tumor specific manner.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"196 ","pages":"Article 102585"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a pescetarian and vegan diet on fatty acid composition in blood and spermatozoa in young healthy men","authors":"Rodrigo Chamorro ,&nbsp;Rut Farías ,&nbsp;Paulina Allende ,&nbsp;Mariana Acuña ,&nbsp;Paula Cáceres ,&nbsp;Fernanda Gómez ,&nbsp;Abigail Ortiz ,&nbsp;Daniela Tapia ,&nbsp;Camila Farías ,&nbsp;Rodrigo Valenzuela","doi":"10.1016/j.plefa.2023.102582","DOIUrl":"10.1016/j.plefa.2023.102582","url":null,"abstract":"<div><h3>Introduction</h3><p>There is a growing interest in vegetarian and vegan diets, but both can potentially affect tissue fatty acids (FA) composition. We aimed to evaluate the effect of vegetarian diets on plasma, erythrocytes, and sperm n-3 polyunsaturated fatty acids (n-3 PUFA) status in healthy young men.</p></div><div><h3>Methods</h3><p>Four groups were studied: <em>i)</em> men consuming a regular omnivore diet (OMV-1, <em>n</em> = 35); <em>ii)</em> men consuming an omnivore diet but excluding fish and seafood (OMV-2, <em>n</em> = 34); <em>iii)</em> men consuming a pescetarian diet (including dairy, eggs, fish, and seafood) (PESC, <em>n</em> = 36); and <em>iv)</em> men following a strict vegan diet (VEG, <em>n</em> = 35). Participants in each group should follow their diet for at least the previous 12 months. Diet evaluation used a structured validated food frequency questionnaire. FA composition was measured in plasma, erythrocyte phospho-lipids, and spermatozoa by gas-liquid chromatography, expressed as a mole percentage of the total FA content.</p></div><div><h3>Results</h3><p>Main findings showed higher alpha-linolenic fatty acid (ALA) and total n-3 PUFA dietary intake in the VEG group. In plasma, arachidonic and eicosapentaenoic acids were higher in OMV and PESC groups, whereas docosahexaenoic acid (DHA) level was lower in VEG. Higher ALA, but reduced DHA and total n-3 PUFA levels were found in erythrocytes and spermatozoa in the VEG group.</p></div><div><h3>Conclusion</h3><p>Higher dietary ALA intake was found in pescetarians and vegan men. However, the higher ALA intake was not reflected in higher DHA content in the evaluated tissues. PUFA assessment, with particular emphasis in DHA, are necessary to improve PUFA status in vegan men.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"196 ","pages":"Article 102582"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erythrocyte fatty acids and desaturase indices in early pregnancy are associated with risk of preeclampsia","authors":"A.A. Godhamgaonkar ,&nbsp;N.S. Wadhwani ,&nbsp;K.N. Randhir ,&nbsp;S.S. Selukar ,&nbsp;S. Dalvi ,&nbsp;K. Dangat ,&nbsp;G.N. Wagh ,&nbsp;S. Lalwani ,&nbsp;N. Chandhiok ,&nbsp;B. Kulkarni ,&nbsp;C. Fall ,&nbsp;H.P.S. Sachdev ,&nbsp;S. Gupte ,&nbsp;S.R. Joshi","doi":"10.1016/j.plefa.2023.102583","DOIUrl":"10.1016/j.plefa.2023.102583","url":null,"abstract":"<div><p>Preeclampsia (PE) is a pregnancy disorder that may be associated with inadequate maternal nutrition. Fatty acids are vital for placental and fetal growth. Fatty acid desaturases, key enzymes influencing the metabolism of polyunsaturated fatty acids, are reported to be associated with cardiometabolic risk. Any imbalance in the levels of omega-3 and omega-6 fatty acids can result in increased inflammatory response. The current study reports the levels of erythrocyte fatty acids and desaturase index across gestation in women who develop PE (<em>n</em> = 108) and compares them with non-PE women (<em>n</em> = 216). Maternal erythrocyte fatty acids were measured at 4 time points during pregnancy (i.e., 11–14, 18–22, 26–28 weeks and at delivery) using gas chromatography. Maternal total erythrocyte saturated fatty acids and omega-6/omega-3 fatty acid ratio was higher in the PE group as compared to the non-PE group at 11–14 weeks and 18–22 weeks respectively. Maternal Δ5 desaturase index was lower while Δ6 desaturase index was higher in the PE group at 11–14 and 18–22 weeks. Maternal stearoyl CoA desaturase-18 (SCD-18) index was lower at 11–14 weeks and at delivery. These changes were mainly observed in the early onset PE (EOP) group. Δ6 desaturase index at 11–14 weeks predicted the risk of EOP. Imbalance in fatty acid levels and desaturase indices predate the clinical diagnosis of PE, indicating their role in its pathophysiology. Measurement of fatty acids and desaturase indices in early pregnancy merits evaluation as predictors of risk of PE.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"196 ","pages":"Article 102583"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10531221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma signatures of Congenital Generalized Lipodystrophy patients identified by untargeted lipidomic profiling are not changed after a fat-containing breakfast meal","authors":"Camilla O.D. Araújo ,&nbsp;Amanda P. Pedroso ,&nbsp;Valter T. Boldarine ,&nbsp;Anna Maria A.P. Fernandes ,&nbsp;José J.M. Perez ,&nbsp;Renan M. Montenegro Jr. ,&nbsp;Ana Paula D.R. Montenegro ,&nbsp;Annelise B. de Carvalho ,&nbsp;Virgínia O. Fernandes ,&nbsp;Lila M. Oyama ,&nbsp;Patrícia O. Carvalho ,&nbsp;Carla S.C. Maia ,&nbsp;Allain A. Bueno ,&nbsp;Eliane B. Ribeiro ,&nbsp;Mônica M. Telles","doi":"10.1016/j.plefa.2023.102584","DOIUrl":"10.1016/j.plefa.2023.102584","url":null,"abstract":"<div><h3>Background</h3><p>The incapacity to store lipids in adipose tissue in Congenital Generalized Lipodystrophy (CGL) causes hypoleptinemia, increased appetite, ectopic fat deposition and lipotoxicity. CGL patients experience shortened life expectancy. The plasma lipidomic profile has not been characterized fully in CGL, nor has the extent of dietary intake in its modulation. The present work investigated the plasma lipidomic profile of CGL patients in comparison to eutrophic individuals at the fasted state and after a breakfast meal.</p></div><div><h3>Method</h3><p>Blood samples from 11 CGL patients and 10 eutrophic controls were collected after 12 h fasting (T0) and 90 min after an <em>ad libitum</em> fat-containing breakfast (T90). The lipidomic profile of extracted plasma lipids was characterized by non-target liquid chromatography mass spectrometry.</p></div><div><h3>Results</h3><p>Important differences between groups were observed at T0 and at T90. Several molecular species of fatty acyls, glycerolipids, sphingolipids and glycerophospholipids were altered in CGL. All the detected fatty acyl molecular species, several diacylglycerols and one triacylglycerol species were upregulated in CGL. Among sphingolipids, one sphingomyelin and one glycosphingolipid species showed downregulation in CGL. Alterations in the glycerophospholipids glycerophosphoethanolamines, glycerophosphoserines and cardiolipins were more complex. Interestingly, when comparing T90 versus T0, the lipidomic profile in CGL did not change as intensely as it did for control participants.</p></div><div><h3>Conclusions</h3><p>The present study found profound alterations in the plasma lipidomic profile of complex lipids in CGL patients as compared to control subjects. A fat-containing breakfast meal did not appear to significantly influence the CGL profile observed in the fasted state. Our study may have implications for clinical practice, also aiding to a deeper comprehension of the role of complex lipids in CGL in view of novel therapeutic strategies.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"196 ","pages":"Article 102584"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10177653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}