Prostaglandins, leukotrienes, and essential fatty acids最新文献

{"title":"Dietary and erythrocyte PUFAs in vegan, lacto-ovo vegetarian, pesco-vegetarian, and non-vegetarian participants of the Adventist Health Study-2","authors":"William Harris ,&nbsp;Roman Pawlak ,&nbsp;Nathan Ryder ,&nbsp;Fayth Miles ,&nbsp;Gary Fraser","doi":"10.1016/j.plefa.2025.102709","DOIUrl":"10.1016/j.plefa.2025.102709","url":null,"abstract":"<div><h3>Background</h3><div>Data on EPA and DHA fatty acids in vegans (VGN) and vegetarians (LOV) are scarce. Also, the extent to which red blood cell (RBC) levels of these fatty acids (FAs) vary by dietary pattern and are correlated with dietary EPA+DHA intake is less well-known.</div></div><div><h3>Objectives</h3><div>The primary objective of this study was to compare RBC EPA and DHA content [i.e., the omega-3 index (O3I)] VGN, LOV, pesco-vegetarians (PV), and non-vegetarians (NV) in the Adventist Health Study II (AHS2). The secondary objective was to determine the extent to which the dietary intake of these PUFAs correlated with RBC levels.</div></div><div><h3>Methods</h3><div>Participants (<em>N</em> = 300, 75 per diet group) were randomly selected from AHS2 cohort. After removing individuals reporting fish oil supplement use, 241 remained for analysis. Dietary data were collected via a validated questionnaire. RBCs were analyzed for FA composition by gas chromatography.</div></div><div><h3>Results</h3><div>Mean O3I (±SD) O3I values across diet groups for VGNs, LOVs, PVs and NVs was 3.7 ± 1.1, 3.9 ± 1.3, 5.7 ± 2.1, and 5.0 ± 1.3, respectively, and EPA+DHA intakes (mg/d) were 10 ± 40, 18 ± 54, 158 ± 279, and 117 ± 91, respectively. EPA+DHA intake correlated strongly with the O3I (<em>r</em> = 0.67, <em>p</em> &lt; 0.001). The dietary ratio of linoleic acid to alpha-linoleic acid was unrelated to the O3I, and the linoleic acid intake was not associated with RBC arachidonic acid levels.</div></div><div><h3>Conclusions</h3><div>VGNs and LOVs had O3I levels (and EPA+DHA intakes) well below recommended targets. For these groups, the most reasonable way to raise these levels is to consume algae-derived EPA+DHA supplements.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"207 ","pages":"Article 102709"},"PeriodicalIF":3.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic sleep deprivation induces depression- and Alzheimer’s disease-like changes in adult and ageing wild-type and Fat-1 transgenic mice","authors":"Nasar Ullah Khan Niazi ,&nbsp;Zhiyou Yang ,&nbsp;Yongping Zhang ,&nbsp;Cai Song","doi":"10.1016/j.plefa.2025.102699","DOIUrl":"10.1016/j.plefa.2025.102699","url":null,"abstract":"<div><h3>Background</h3><div>Sleep disorders show comorbidity with depression and Alzheimer's disease (AD), especially in ageing. However, the neuroimmunological role of sleep deprivation (SD) as possible inducer to these conditions remains unknown. Omega-3 fatty acids (n-3 FAs) can improve depression and AD through anti-inflammation, up-regulating neurotrophins and normalizing neurotransmitters, while their therapeutic effects on sleep deprivation (SD)-induced changes in different ages requires investigation.</div></div><div><h3>Methods</h3><div>Adult and old Fat-1 (converting n-6 to n-3 FAs) and wild-type (WT) mice were subjected to chronic SD. After behavioral evaluation, brain FAs, monoamine neurotransmitters, circadian-gene expression, TLR-4 signaling-pathway, glial polarization, cytokine profile, and AD-related markers were analyzed using GC–MS, HPLC, qPCR, ELISA and western-blotting. Furthermore, bioinformatic analysis evaluated SD-related networking with depression and AD.</div></div><div><h3>Results</h3><div>SD induced anxiety, anhedonia, despair, and memory impairments. The n-3:n-6 ratio, BMAL-1 gene expression, and melatonin concentration were decreased, whereas corticosterone, TLR-4, GSK3β, and NFκB concentrations increased in SD groups compared to the controls. Increased IBA-1 protein expression and proinflammatory IL-1β, TNF-α, and IL-6 concentrations were associated with decreased monoamine neuro-transmitter levels in SD groups. APP, BACE-1, RAGE and APPβ concentrations were increased, whereas LRP-1 and APPα concentrations and the APPα/APPβ ratio were decreased in SD groups than controls. These changes were more pronounced in old WT and Fat-1 animals than adults. However, compared to WT-SD, these changes were significantly ameliorated in Fat-1-SD mice, but recovery was less pronounced in old Fat-1.</div></div><div><h3>Conclusion</h3><div>SD-induced neuroinflammation and impaired APP processing may contribute to behavioral impairments, which exacerbated with age. Although n-3 FAs significantly ameliorated SD-induced adverse behavioral and neuroimmunological changes, this therapeutic effect was markedly reduced in old animals.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"207 ","pages":"Article 102699"},"PeriodicalIF":3.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal blood glucose level is correlated to offspring brain fatty acid composition in a mouse model for gestational diabetes mellitus","authors":"Lidewij Schipper ,&nbsp;Noela Schaap ,&nbsp;Yixian Liu ,&nbsp;Hongyu Li ,&nbsp;Weiping Han ,&nbsp;Louise Harvey","doi":"10.1016/j.plefa.2025.102700","DOIUrl":"10.1016/j.plefa.2025.102700","url":null,"abstract":"<div><div>Gestational diabetes mellitus (GDM) may increase the risk of suboptimal neurocognitive development in infants. Maternal supply of omega-3 and -6 long chain polyunsaturated fatty acids (LCPUFAs), including eicosapentaeonic acid, docosahexaenoic acid and arachidonic acid, are critical for offspring brain development. To study the effects of GDM on offspring brain fatty acid composition, C57BL/6J mice were exposed to short-term high-fat diet feeding and low-dose streptozotocin treatments before pregnancy. Maternal blood glucose levels positively correlated to offspring brain omega 6:omega 3 ratio at postnatal day 2 and day 21, which appeared to be driven specifically by higher omega-6 LCPUFA levels. GDM may be associated with impaired brain fatty acid profile in offspring, and this may underpin altered neurodevelopmental outcomes after GDM pregnancies. These findings support further investigation into the therapeutic potential of postnatal dietary interventions targeting fatty acid status in infants born after GDM.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102700"},"PeriodicalIF":3.2,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the complex interplay between zinc, iron and their synergistic effect on LCPUFA metabolism in preeclampsia","authors":"Vaishnavi Mudgal,&nbsp;Juhi Nema,&nbsp;Sadhana Joshi","doi":"10.1016/j.plefa.2025.102701","DOIUrl":"10.1016/j.plefa.2025.102701","url":null,"abstract":"<div><div>Preeclampsia is a hypertensive disorder of pregnancy. It is a leading cause of maternal mortality and morbidity. Long-chain polyunsaturated fatty acids (LCPUFAs) are critical for normal placental and fetal development. Biosynthesis of these LCPUFAs is regulated by Δ5 and Δ6 desaturase enzymes, which require zinc and iron as cofactors. Both zinc and iron are important micronutrients which play pivotal role during pregnancy and their deficiency during pregnancy is linked with preeclampsia. Zinc and iron deficiency is common in pregnancy and often coexists. Zinc and iron interact in complex ways, influencing the bioavailability of each other. On the other hand, zinc is required for intestinal uptake and transport of iron. The current article summarizes studies examining the role of zinc, iron and LCPUFAs in preeclampsia. It also gives a mechanistic insight through which zinc and iron synergistically influence LCPUFA biosynthesis in pregnancy complicated with preeclampsia.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102701"},"PeriodicalIF":3.2,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144885393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive role of erythrocyte fatty acids in conversion to psychosis: a longitudinal study of clinical high-risk individuals","authors":"LiHua Xu ,&nbsp;YanYan Wei ,&nbsp;XiaoChen Tang ,&nbsp;ZhengHui Yi ,&nbsp;XiaoHua Liu ,&nbsp;HaiChun Liu ,&nbsp;ZiXuan Wang ,&nbsp;Tao Chen ,&nbsp;Jin Gao ,&nbsp;Qiang Hu ,&nbsp;LingYun Zeng ,&nbsp;ChunBo Li ,&nbsp;JiJun Wang ,&nbsp;TianHong Zhang","doi":"10.1016/j.plefa.2025.102698","DOIUrl":"10.1016/j.plefa.2025.102698","url":null,"abstract":"<div><h3>Introduction</h3><div>Fatty acid (FA) alterations are associated in patients with psychosis. This study aimed to investigate the role of FAs in predicting the conversion to psychosis in individuals at clinical high-risk (CHR) and to distinguish CHR individuals from healthy controls (HC).</div></div><div><h3>Materials and methods</h3><div>A three-year longitudinal study was conducted involving 67 CHR individuals and 25 HC participants. Comprehensive erythrocyte FA profiles were obtained from gas chromatography analysis at baseline, measuring 17 different FAs. Participants were followed for three years to determine outcomes, categorizing CHR individuals into converters to psychosis (CHR-C) and non-converters (CHR-NC). We employed LASSO regression models to identify key predictors for CHR-C from CHR and to distinguish CHR from HC. Receiver operating characteristic (ROC) curve analyses were performed to evaluate the models' predictive and discriminative abilities. Significant differences in FA profiles were observed among HC, CHR-C, and CHR-NC groups.</div></div><div><h3>Results</h3><div>CHR-C individuals exhibited higher levels of Eicosenoic acid(<em>p</em> = 0.016), Linoleic acid(<em>p</em> = 0.009), Eicosadienoic acid(<em>p</em> = 0.035) and Arachidonic acid(<em>p</em> = 0.037) compared to CHR-NC, whereas CHR individuals showed elevated levels of Palmitoleic acid(<em>p</em> = 0.002), Palmitic acid(<em>p</em> = 0.012), Eicosapentaenoic acid(<em>p</em> = 0.019) and Oleic acid(<em>p</em> = 0.039) compared to HC. The LASSO model identified key sociodemographic and FA predictors, including age, education, and specific FAs, for distinguishing CHR from HC, demonstrating high discriminative ability with an area under the ROC curve(AUC) of 0.894. For predicting CHR-C, the model highlighted gender and specific ω-6 FAs as potential predictors, with an AUC of 0.801.</div></div><div><h3>Discussion</h3><div>Our findings reveal distinct FA profiles among HC, CHR-C, and CHR-NC groups and identify key predictors for psychosis conversion and CHR status. These results underscore the significance of FAs in the various stages of psychosis development.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102698"},"PeriodicalIF":3.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex and dietary ALA and DHA effects on rat heart phospholipase A2 activity mediating fatty acid release and oxylipin formation have cardiovascular implications","authors":"Anne Manson ,&nbsp;Tanja Winter ,&nbsp;Harold M. Aukema","doi":"10.1016/j.plefa.2025.102697","DOIUrl":"10.1016/j.plefa.2025.102697","url":null,"abstract":"<div><div>The ways in which diet and sex influence heart phospholipase A₂ (PLA₂) activities are underexplored, particularly with respect to effects on oxylipin formation. Therefore, male and female Sprague-Dawley rats were provided diets with 1.3 % α-linolenic acid (ALA) or docosahexaenoic acid (DHA) for 6 weeks, and heart homogenates were incubated with inhibitors for secreted (s)PLA₂ (Varespladib) or calcium-independent (i)PLA₂ (methyl arachidonyl fluorophosphonate). Non-esterified polyunsaturated fatty acids (PUFA) and oxylipins were determined by HPLC-MS/MS and data were analyzed using 3-way repeated measures ANOVA. sPLA₂ activity was selective for DHA release and DHA oxylipin formation, and contributed to ∼50 % of arachidonic acid (ARA) release, but only ∼30 % of ARA oxylipin formation. Eicosapentaenoic acid (EPA) release and oxylipin formation was minor but primarily mediated by iPLA₂. iPLA₂ activity was less selective, with activity towards all measured PUFA and oxylipins, with the primary PUFA released depending on diet. For both sPLA₂ and iPLA₂, there was more activity towards DHA and its oxylipins with the DHA diet, and towards ALA and ARA and their oxylipins with the ALA diet. Sex effects on fatty acids and oxylipins differed, as DHA, EPA and ALA release were higher in females, while DGLA and cyclooxygenase-derived ARA oxylipin formation were higher in males. Interestingly, the sex effect on cyclooxygenase ARA oxylipins was attenuated with dietary DHA. These findings provide further rationale for the simultaneous measurement of PUFA and oxylipins since they are not always congruent, and shed new light on diet and sex effects on PLA₂ types in cardiovascular oxylipin biology.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102697"},"PeriodicalIF":3.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of biological sex on skeletal muscle phospholipid membrane composition in response to omega n-3 polyunsaturated fatty acid supplementation and washout in humans","authors":"Callum J. Pufahl ,&nbsp;Sydney E. Smart ,&nbsp;Justin Bureau ,&nbsp;Brendan Ross ,&nbsp;Craig A. Simpson ,&nbsp;Adam H. Metherel ,&nbsp;James R. Dick ,&nbsp;Chris McGlory","doi":"10.1016/j.plefa.2025.102696","DOIUrl":"10.1016/j.plefa.2025.102696","url":null,"abstract":"<div><div>Omega n-3 polyunsaturated fatty acid (n-3 PUFA) intake is associated with reduced inflammation, protection against cardiovascular disease, and improved cognitive function. There is also evidence that n-3 PUFA intake impacts skeletal muscle, potentially with greater effects in females than males. Sex-specific responses to n-3 PUFA intake may be linked to the incorporation of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) into skeletal muscle phospholipid membranes. However, no study has characterized the effect of biological sex on human skeletal muscle phospholipid composition in response to EPA+DHA supplementation, and washout of EPA and DHA from skeletal muscle phospholipids following the cessation of supplementation. In a repeated measures design, 15 females and 14 males consumed 5 g/d of EPA+DHA (3.75 g EPA; 1.25 g DHA) for 8 weeks followed by 14 weeks of washout. Skeletal muscle biopsies and venous blood samples were obtained at weeks 0 (baseline), 6, 8, 16, 20, and 22. Females displayed greater skeletal muscle EPA phospholipid composition (<em>p</em>= 0.035) at 6 weeks of supplementation, higher erythrocyte EPA phospholipid composition (<em>p</em>= 0.034), and whole plasma DHA composition (<em>p</em>= 0.033) compared to males. At 14 weeks of washout, skeletal muscle and erythrocyte EPA phospholipid composition (<em>p</em>&lt; 0.001 and <em>p</em>= 0.005) remained elevated above baseline, whereas whole plasma EPA composition (<em>p</em>= 0.381) was not different from baseline. We demonstrate that 5 g/d EPA+DHA supplementation influences human fatty acid composition in a manner that is both sex- and tissue-dependant.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102696"},"PeriodicalIF":3.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linoleic acid promotes mammary tumor growth and metastasis to brain and lung in female Balb/cJ mice","authors":"Maria Sanchez-Juarez ,&nbsp;Monica Vizcarra-Soto ,&nbsp;Rocio Castillo-Sanchez ,&nbsp;Pablo Torres-Alamilla ,&nbsp;Pedro Cortes-Reynosa ,&nbsp;Gustavo Acosta-Altamirano ,&nbsp;Monica Sierra-Martinez ,&nbsp;Eduardo Perez Salazar","doi":"10.1016/j.plefa.2025.102687","DOIUrl":"10.1016/j.plefa.2025.102687","url":null,"abstract":"<div><div>Breast cancer is the leading cause of mortality among women worldwide. The largest prevalence of breast cancer is present in high-income nations, but the incidence in low- to middle-income countries has risen in recent years, which is the consequence of various causes, such as dietary habits. Dietary fat intake is a factor associated with the risk of developing breast cancer, and a moderate positive association between n-6 fatty acids and breast cancer risk has been described. Linoleic acid (LA) is an omega-6 polyunsaturated fatty acid (PUFA), which represents an essential PUFA and the major fatty acid consumed in occidental diets. It has been demonstrated that LA promotes cellular processes involved with invasion/metastasis in MDA-MB-231 breast cancer cells. In this study, we demonstrate that LA induces migration via FFAR1 and FFAR4, invasion and secretion of matrix metalloproteinase (MMP)-2 and MMP-9 in 4T1 triple negative breast cancer cells. In addition, 4T1 cells treated with 60 µM LA for 7 days and then inoculated in Balb/cJ mice induces an increase in the weight and volume of mammary tumors, and an increase in the metastasis to brain and liver compared with Balb/cJ mice inoculated with untreated 4T1 cells. In conclusion, LA induces cellular processes involved with invasion/metastasis and an increase in the growth of mammary tumors and metastasis in a murine model of breast cancer using Balb/cJ mice and 4T1 cells.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102687"},"PeriodicalIF":3.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of n-3 polyunsaturate fatty acids supplementation on visual health throughout the life cycle: A systematic review","authors":"Claudia Tabilo ,&nbsp;Valentina Squella ,&nbsp;Paola Illesca ,&nbsp;Yasna Muñoz ,&nbsp;Camila Farías ,&nbsp;Rodrigo Valenzuela","doi":"10.1016/j.plefa.2025.102686","DOIUrl":"10.1016/j.plefa.2025.102686","url":null,"abstract":"<div><h3>Introduction</h3><div>N-3 polyunsaturated fatty acids (n-3 PUFA) are important for mammals and have relevant functions in the body. These fatty acids play an important role in brain development, in the protection of the retina, and in the prevention of macular degeneration. Currently, clinical trials do not yet confirm a clear benefit of n-3 PUFA supplementation on vision throughout the life cycle. Therefore, the aim of this study is to systematically evaluate the available scientific evidence to determine the effects of n-3 PUFA supplementation on visual health throughout the life cycle.</div></div><div><h3>Material and Methods</h3><div>A search of scientific literature was performed, based on randomized, controlled clinical studies, published in PubMed, using keywords. We included people of both sexes throughout the life cycle that evaluated the impact of n-3 PUFA supplementation on visual health.</div></div><div><h3>Results</h3><div>Of the 87 articles included in this review, there are important contradictions in the literature regarding the effects of supplementation in pregnant women, infants and older adults. While some studies highlight beneficial effects, an equal number of studies report no impact. In the case of preschoolers and schoolchildren, predominantly positive effects were identified, especially in children with Attention Deficit Hyperactivity Disorder (ADHD). For youth and adults, the impact of supplementation varied according to the health condition assessed, mostly supporting significant benefits in individuals with dry eye symptoms.</div></div><div><h3>Conclusion</h3><div>Evidence supports supplementation with n-3 PUFA, especially docosahexaenoic acid (DHA), to improve visual development in infants, schoolchildren, especially those with ADHD. Despite variability in visual outcomes, these findings suggest a crucial role of n-3 PUFA supplementation in visual health in the first steps of life, but in adults and older adults remain uncertain or null. Therefore, it is critical to investigate optimal dosing, duration of the intervention as the age of the participants in future research.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102686"},"PeriodicalIF":3.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144253997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysfunction of COX-2/mPGES-1/PGE2 pathway and EP4 receptor in bronchi from COPD patients","authors":"Salma Mani ,&nbsp;Zhipeng Li ,&nbsp;Hichem Badji ,&nbsp;Gaelle Merheb ,&nbsp;Sébastien Dupont ,&nbsp;Yves Castier ,&nbsp;Olivier Thibaudeau ,&nbsp;Mathilde Varret ,&nbsp;Alice Guyard ,&nbsp;Dan Longrois ,&nbsp;Xavier Norel","doi":"10.1016/j.plefa.2025.102685","DOIUrl":"10.1016/j.plefa.2025.102685","url":null,"abstract":"<div><div>Progressive airflow obstruction and chronic lung inflammation are hallmarks of chronic obstructive pulmonary disease (COPD). Prostaglandin E2 (PGE₂), synthesized by the cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1), acts as a lipid mediator with bronchodilatory effects mediated by the EP4 receptor. Altered expression and function of COX-2, mPGES-1, PGE₂ and EP receptors may contribute to the pathophysiology of COPD. This study investigates whether COPD is associated with dysregulated expression or function of COX-2, mPGES-1, EP receptors and PGE₂ production in human bronchi. We analyzed the expression of COX-2, mPGES-1, PGE₂ and EP receptors in human bronchi samples using Western blot, real-time qPCR, ELISA and immunohistochemistry (IHC). Our results reveal significantly elevated COX-2 protein, mPGES-1 mRNA, and PGE₂ levels in COPD patients compared to controls. Conversely, in COPD preparations EP4 receptor mRNA and protein levels were markedly reduced, a result confirmed by IHC. In addition, IHC also showed that the EP4 receptor was mainly localized in the epithelium of control bronchi. Notably, there was a significant negative correlation between EP4 and PGE₂ levels. The hypothesis of EP4 internalization due to increased PGE₂ in COPD patients is credible. These data demonstrate a significant alteration of the COX-2/mPGES-1/PGE₂/EP4 pathway in COPD and suggest that pharmacological targeting of this pathway may be of interest to treat COPD.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"206 ","pages":"Article 102685"},"PeriodicalIF":3.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}