Journal of materials chemistry. B最新文献

{"title":"MnCO₃-Au nanoparticles to enable catalytic tumor inhibition with immune activation.","authors":"Yingpei Yao, Zijie Lu, Yike Fu, Xiang Li","doi":"10.1039/d4tb02108h","DOIUrl":"10.1039/d4tb02108h","url":null,"abstract":"<p><p>Catalytic nanomedicine, activated by endogenous stimuli to enable specific tumor inhibition, has attracted extensive interest in recent years. However, its therapeutic outcomes are often restrained by the weakly acidic microenvironment and limited H₂O₂ endogenous content. Here, in this study, gold nanoparticles (AuNPs) with glucose oxidase-like activity are incorporated with biodegradable MnCO₃ nanoparticles. AuNPs catalyze glucose oxidation to generate gluconic acid and H₂O₂, while MnCO₃ is degraded by the generated gluconic acid as well as the acidic conditions in the tumor region to release Mn²⁺ and HCO₃^-. Then H₂O₂ can be catalyzed by Mn²⁺ and HCO₃^- to produce reactive oxygen species (ROS). The effective production of on-site H₂O₂ leads to promoted intracellular ROS and enhanced tumor inhibition. More importantly, the released Mn²⁺ ions not only act as a catalytic agent, but also serve as a stimulator of the cGAS-STING pathway to activate anti-tumor immune responses. The <i>in vivo</i> study confirms that MnCO₃-Au promotes T cell infiltration in tumors and exhibits a synergistic tumor suppression effect. This study may provide an alternative protocol for combinational tumor therapy utilizing the dual roles of Mn²⁺ as an emerging catalytic agent as well as an immune agonist.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Instant synthesis of nitrogen-doped Ti₃C₂ MXene quantum dots for fluorescence and electrochemical dual-mode detection of norepinephrine with a portable smartphone assay.","authors":"Murugesan Chandran, Gayathri Chellasamy, Mekala Veerapandian, Barkavi Dhanasekaran, Saravanan Govindaraju, Kyusik Yun","doi":"10.1039/d4tb01818d","DOIUrl":"https://doi.org/10.1039/d4tb01818d","url":null,"abstract":"<p><p>Next-generation 2D materials, such as transition metal carbides and nitrides (MXenes), have received increasing attention owing to their physicochemical properties. In this study, we synthesized highly intense fluorescent materials, nitrogen-doped MXene quantum dots (N-MQDs) using an easy and less time-consuming microwave-assisted method. These N-MQDs are spherical, fluorescent, and highly sensitive materials, as confirmed by high-resolution transmission electron microscopy, atomic force microscopy, UV-visible, fluorescence, Fourier transform infrared spectroscopy, X-ray diffraction, Raman spectroscopy, zeta potential, and contact angle measurements. The N-MQDs were used as dual probes for the fluorescence and electrochemical sensing of neurotransmitter norepinephrine (NE-0.1 to 500 μM). The sensing strategy is based on the Förster resonance energy transfer acquired by the N-MQDs, leading to fluorescence quenching at 400 nm. A new emission peak at 500 nm with color changes and NE-to-NE quinone conversion in an electrochemical reaction. Fluorescence and electrochemical analyses were revealed using the human serum sample limit of detection (LOD) values of 40 and 33 nM, respectively. For point-of-care analysis, we developed a smartphone-integrated sensor array to calculate intensity changes, and the relative red/green/blue (RGB) values were measured at different concentrations of NE. The synthesized fluorescent probe is a promising candidate for detecting NE in biofluids. It is highly selective toward NE and is suitable for the early diagnosis of neurological diseases.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From natural to synthetic hydrogels: how much biochemical complexity is required for mechanotransduction?","authors":"Johnick F van Sprang, Imke P M Smits, Jasper C H Nooten, Peter-Paul K H Fransen, Serge H M Söntjens, Michel H C J van Houtem, Henk M Janssen, Martin G T A Rutten, Maaike J G Schotman, P Y W Dankers","doi":"10.1039/d4tb01774a","DOIUrl":"https://doi.org/10.1039/d4tb01774a","url":null,"abstract":"<p><p>The biochemical complexity of a material determines the biological response of cells triggered by a cell-material interaction. The degree in which this complexity influences basic cell-material interactions such as cell adhesion, spreading, and mechanotransduction is not entirely clear. To this end, we compared three different hydrogel systems, ranging from completely natural to synthetic, in their ability to induce mechanotransduction in kidney epithelial cells (HK-2). A natural hydrogel system was developed based on a decellularized kidney extracellular matrix (dECM). Supramolecular ureido-pyrimidinone (UPy)-glycinamide molecules, with self-associative behavior, were used for a hybrid and complete synthetic system. A hybrid system was engineered by co-assembling this monovalent UPy molecule with a hyaluronic acid, functionalized with ∼7 UPy-groups (UPy-HA), into a transient network. A similar approach was used for the synthetic hydrogel system, in which the multivalent UPy-HA was replaced with a bivalent UPy-PEG molecule with bioinert properties. Both hybrid and synthetic hydrogel systems were more mechanically tunable compared to the dECM hydrogel. The higher bulk stiffness in combination with the introduction of collagen type I mimicking UPy-additives allowed these materials to induce more nuclear yes-associated protein translocation in HK-2 cells compared to the biochemically complex dECM hydrogel. This demonstrated that minimal biochemical complexity is sufficient for inducing mechanotransduction.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic microwave hyperthermia treatment for subcutaneous deep <i>in situ</i> breast cancer using conformal array antennas and a microwave-thermal-sensitive nanomaterial.","authors":"Xinyu Zhang, Yongxing Du, Ling Qin, Baoshan Li, Qiong Wu, Xianwei Meng","doi":"10.1039/d4tb02319f","DOIUrl":"10.1039/d4tb02319f","url":null,"abstract":"<p><p>When microwave hyperthermia (MWH) array antenna technology is used to treat breast cancer, how to effectively target and heat deep tumors and reduce thermal damage to healthy tissues is still a challenge in clinical applications. In this study, the synergistic MWH effect of conformal-array antennas (CAA) and a novel microwave-thermal-sensitive nanomaterial (MTSN) was investigated for the treatment of subcutaneous deep <i>in situ</i> breast cancer. At the beginning of the study, the thermal damage score was used to evaluate the therapeutic efficacy of the CAA. It was found that although array antenna technology can achieve effective heating of deep tumors, its damage to healthy tissues is unacceptable. Consequently, we developed a novel MTSN, ZIF-8@HA, whose unique structure significantly enhanced the absorption of MW energy and MW thermal conversion efficiency in the local tumor region. The MW thermal conversion efficiency of ZIF-8@HA achieved was as high as 46.46% in <i>in vivo</i> MW heating experiments. In the phantom that simulates the electromagnetic environment of the human body, the microwave-thermal sensitization (MTS) effect is also significant, and the reduction in the average thermal damage score of healthy tissues by more than 10% was verified through measurements using the coaxial probe method and COMSOL simulations. Cellular experiments confirmed that the combination of ZIF-8@HA and MW irradiation could significantly reduce the survival rate of tumor cells. In addition, cross-tissue MW heating experiments revealed the advantages of ZIF-8@HA combined with the CAA. Finally, phantom experiments confirmed that the synergistic use of the CAA with ZIF-8@HA significantly accelerated the local heating rate of deep tumors, reduced the time required for the tumor region to achieve 100% thermal damage, and effectively minimized the thermal damage to healthy tissues.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylidene-based dual-responsive 'turn on' fluorometric chemosensors for the selective detection of Zn²⁺, Al³⁺ and F^- ions: theoretical investigation and applications in the live cell imaging of zebrafish larvae and molecular logic gate operation.","authors":"Abbas Khaja Raees Ahmed, Ramalingam Gajendhiran, Sivaraj Mithra, Seepoo Abdul Majeed, Azeez Sait Sahul Hameed, Rajakkani Paulpandiyan, Subbaiah Maniyammai, Gurusamy Thangavelu Senthil Andavan, MohamedHanifa NizamMohideen, Aziz Kalilur Rahiman","doi":"10.1039/d4tb01356e","DOIUrl":"https://doi.org/10.1039/d4tb01356e","url":null,"abstract":"<p><p>Four salicylidene-based dual-responsive chemosensors 1,5-bis(5-bromosalicylaldehyde)carbohydrazone (R1), 1,5-bis(5-bromosalicylaldehyde)thiocarbohydrazone (R2), 1,5-bis(3-ethoxysalicylaldehyde)carbohydrazone (R3) and 1,5-bis(3-ethoxysalicylaldehyde)thiocarbohydrazone (R4) were synthesized and characterized. The molecular structures of R1 and R3 were confirmed by single crystal X-ray diffraction technique, which crystallized in the orthorhombic <i>Pbcn</i> and monoclinic <i>P</i>2₁/<i>n</i> space groups, respectively. The chemosensor molecules were investigated for their recognition properties against the selected cations (K⁺, Ca²⁺, Mn²⁺, Co²⁺, Ni²⁺, Cu²⁺, Zn²⁺, Fe³⁺ and Al³⁺) and anions (F^-, Cl^-, Br^-, I^-, HSO₄^-, H₂PO₄^-, ClO₄^-, N₃^- and NO₃^-) by colorimetry, absorption spectroscopy, fluorescence spectroscopy, ¹H NMR spectroscopy and theoretical studies. The sensor molecules showed colorimetric responses for the Co²⁺, Ni²⁺, Cu²⁺ and Fe³⁺ cations and the F^- anion. Interestingly, the Zn²⁺ and Al³⁺ cations showed only the 'turn on' fluorometric response, whereas the F^- anion showed both colorimetric and fluorometric responses. The binding constants were determined using the Benesi-Hildebrand (B-H) equation from the fluorescence titrations and found to be higher for R3 towards the Al³⁺ cation (2.03 × 10⁶ M^-1) with a low limit of detection (1.79 μM) and for R4 towards the F^- anion (5.13 × 10⁵ M^-1) with a low limit of detection (5.23 μM). The chemosensors established 1 : 2 and 1 : 1 binding stoichiometries with the sensed cations and anion, respectively, as confirmed by Job's plots. The computational studies show a lower band gap of HOMO-LUMO when the chemosensors bind with the sensed inorganic ions compared to the free chemosensors. Furthermore, the observed fluorescent behaviour of the Zn²⁺ and Al³⁺ cations have motivated us to investigate the practical applications in the live cell-imaging of zebrafish larvae as well as in the development of a molecular logic gate.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in phasor-based FLIM: multi-component analysis and lifetime probes in biological imaging.","authors":"Dan Li, Xinyi Liu, Fanli Dong, Wanwan Li","doi":"10.1039/d4tb01669f","DOIUrl":"10.1039/d4tb01669f","url":null,"abstract":"<p><p>Fluorescence lifetime imaging microscopy (FLIM) is a reliable method that achieves imaging by detecting fluorescence lifetimes within samples. Owing to its unique temporal characteristic, it can complement fluorescence intensity measurement. Technological and methodological advancements in FLIM have broadened its applications across various domains. The processing of fluorescence lifetime data is crucial for enhancing the speed and accuracy of imaging. Thus, various lifetime fitting algorithms have been developed to improve the imaging speed. The phasor analysis (PA) method is an approach for processing fluorescence lifetime data, capable of directly converting lifetime signals into visual graphics without fitting, which outperforms traditional approaches in speed. Furthermore, lifetime probes with distinct lifetimes are readily implemented for visualization and cluster analysis combined with PA, facilitating the prediction of specific biological states or functions. This review examines various lifetime probes employed in phasor-based FLIM and discusses their roles in the PA method. The methods for multi-component PA within complex biological environments were also described. Additionally, we focused on the advantages of the phasor vector rule and the unmixing of multi-component analysis based on PA. The integration of lifetime probes with phasor-based FLIM facilitates rapid and intuitive detection methods for analyzing complex biological environments.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in polymer-based thin-film electrodes for ECoG applications.","authors":"Zhengchen Xiang, Liangtao Yang, Bin Yu, Qi Zeng, Tao Huang, Shuo Shi, Hao Yu, Yi Zhang, Jinglong Wu, Meifang Zhu","doi":"10.1039/d4tb02090a","DOIUrl":"10.1039/d4tb02090a","url":null,"abstract":"<p><p>Electrocorticography (ECoG) has garnered widespread attention owing to its superior signal resolution compared to conventional electroencephalogram (EEG). While ECoG signal acquisition entails invasiveness, the invasive rigid electrode used inevitably inflicts damage on brain tissue. Polymer electrodes that combine conductivity and transparency have garnered great interest because they not only facilitate high-quality signal acquisition but also provide additional insights while preserving the health of the brain, positioning them as the future frontier in the brain-computer interface (BCI). This review summarizes the multifaceted functions of polymers in ECoG thin-film electrodes for the BCI. We present the abilities of sensitive and structural polymers focusing on impedance reduction, signal quality improvement, good flexibility, and transparency. Typically, two sensitive polymers and four structural polymers are analyzed in detail in terms of ECoG electrode properties. Moreover, the underlying mechanism of polymer-based electrodes in signal quality enhancement is revealed. Finally, the remaining challenges and perspectives are discussed.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a diagnostic and drug evaluation system for acute inflammation using a novel [⁸⁹Zr]DTPA-sorbitol probe.","authors":"Seung Ho Baek, Eun-Ha Hwang, Sang Bong Lee, Miji Kim, Dong-Yeon Kim, Jung Joo Hong, Kyung-Sun Kang","doi":"10.1039/d4tb02061h","DOIUrl":"10.1039/d4tb02061h","url":null,"abstract":"<p><p>Non-invasive imaging techniques employing biomarkers with high selectivity for inflammation are essential not only for the early diagnosis and prevention of chronic inflammatory diseases but also for guiding appropriate drug therapy and enabling real-time evaluation of anti-inflammatory drug efficacy. In this study, we conjugated radioactive zirconium to sorbitol, a compound that can selectively target inflammation, and evaluated its inflammation-specific uptake and potential for assessing anti-inflammatory treatment efficacy in a mouse inflammation model. Pharmacokinetic analysis demonstrated that radiolabeled sorbitol achieved maximal uptake in inflamed tissues within 1 h. Positron emission tomography imaging further confirmed its utility in monitoring therapeutic effects during anti-inflammatory drug treatment. Our findings suggest that [⁸⁹Zr]DTPA-sorbitol is a promising radioprobe for targeting rapid systemic inflammation, particularly in tissues demonstrating minimal non-specific uptake, such as the brain, heart, and lung tissues. Additionally, it holds significant potential for the <i>in vivo</i> evaluation of anti-inflammatory drug efficacy.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired programmable coacervate droplets and self-assembled fibers through pH regulation of monomers.","authors":"Satyajit Patra, Sushmitha Chandrabhas, Subi J George","doi":"10.1039/d4tb01550a","DOIUrl":"10.1039/d4tb01550a","url":null,"abstract":"<p><p>Phase separation and phase transitions pervade the biological domain, where proteins and RNA engage in liquid-liquid phase separation (LLPS), forming liquid-like membraneless organelles. The misregulation or dysfunction of these proteins culminates in the formation of solid aggregates <i>via</i> a liquid-to-solid transition, leading to pathogenic conditions. To decipher the underlying mechanisms, synthetic LLPS has been examined through complex coacervate formation from charged polymers. Nonetheless, temporal control over phase transitions from prebiotically relevant small organic synthons remains largely unexplored. Herein, we propose utilizing pH modulation to regulate the charge of small molecular building blocks, thereby controlling the LLPS process. Through a bio-inspired, enzyme-mediated pH-regulated reaction, we introduce temporal control over both LLPS and the transition from coacervates to supramolecular polymers. Additionally, by incorporating antagonistic pH modulators, we achieve transient LLPS and further temporal regulation of supramolecular polymer disassembly. Our investigation into pH-regulated LLPS provides a new avenue for exploring the stimuli-responsive, dynamic, and transient nature of LLPS.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly self-cleaning coatings: fundamentals, fabrication, applications, and sustainability.","authors":"Tanaji K Chavan, Sushanta K Sethi","doi":"10.1039/d4tb01392a","DOIUrl":"10.1039/d4tb01392a","url":null,"abstract":"<p><p>Eco-friendly self-cleaning coatings have garnered significant attention due to their potential to address environmental concerns while offering remarkable properties. This review explores the dynamic field of such coatings, focusing on their fundamental principles, fabrication techniques, applications, and sustainability. The main findings of this review shed light on the fundamentals of a wetting phenomenon that underpins superhydrophobicity and self-cleaning, revealing how bio-inspired approaches and sustainable materials have enabled the development of sustainable coatings. This review is structured around the fundamental principles of superhydrophobicity, discussing the basic mechanisms and following different approaches to eco-friendly coatings, focusing on bio-inspired methods and sustainable materials. Next, detailed fabrication techniques are discussed to create such coatings followed by various applications across industries, emphasizing the real-world impact of eco-friendly coatings. The next section discusses the various advantages followed by investigating the environmental implications and discussing how these coatings contribute to sustainability. The review concludes with commercial superhydrophobic self-cleaning products, which reflect the current state of research, outlining the challenges, and providing insights into future directions and innovations in this field. By providing an in-depth analysis of their fabrication techniques, applications, and potential future directions, it serves as a valuable resource for researchers and engineers seeking to design eco-friendly superhydrophobic coatings.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142690107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}