Journal of materials chemistry. B最新文献

{"title":"Polyurethane packed graphene-coated spider silk by dip-casting for a highly stretchable strain sensor.","authors":"Zaigham Abbas, Gul Hassan, Muhammad Umair Khan, Haider Abbas, Basheer Ahmad, Ahmed Shuja, Memoon Sajid, Jinho Bae, Changhwan Choi","doi":"10.1039/d4tb01164c","DOIUrl":"https://doi.org/10.1039/d4tb01164c","url":null,"abstract":"<p><p>In recent years, naturally occurring materials have gained tremendous attention for their potential in the fabrication of advanced wearable electronic devices. Among these materials, spider silk is well-known for its outstanding mechanical strength and physical properties. Leveraging these attributes, a highly stretchable strain sensor was developed in this work utilizing polyurethane packed graphene-coated spider silk fabricated through a simple dip-casting method. The proposed sensor demonstrated remarkable mechanical strength, excellent sensitivity to strain and impressive recovery properties, attributed to the self-healing abilities of the polyurethane packaging. Additionally, polyurethane served as a protective layer, safeguarding the sensor from external environmental parameters and improving the lifetime of the device. The fabricated devices retained their performance parameters and other properties at up to 40% stretchability. FE-SEM and EDS elemental mapping were used to confirm the morphological properties and the homogenous coating of graphene on the spider silk fibers. The sensors were then attached to different parts of the human body to monitor pulse-rate, joint angles and posture, demonstrating outstanding results. This work paves the way for the design and fabrication of future wearable sensors using naturally occurring materials for advanced electronic applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A piezoelectric hydrogel containing bismuth sulfide with cationic vacancies with enhanced sonodynamic/nanozyme activity for synergistically killing bacteria and boosting osteoblast differentiation.","authors":"Xiaowen Xi, Susu Ma, Ping Sun, Zhitao Hu, Jie Wei, Yunfei Niu","doi":"10.1039/d4tb02693d","DOIUrl":"https://doi.org/10.1039/d4tb02693d","url":null,"abstract":"<p><p>A piezoelectric nanozyme is a novel biomaterial with the integration of piezoelectricity and nanozyme activity that has the capability of killing bacteria and promoting cell responses under a mechanical stimulus and exhibits great prospects in tissue regeneration. Herein, a piezoelectric nanozyme of bismuth sulfide (BS) with cationic vacancies (VBS) was synthesized, which exhibits enhanced piezoelectricity and nanozyme activities compared with BS. Moreover, a piezoelectric hydrogel of VBS and phenylboronic acid grafted sodium alginate-arginine (VBS-PSA) was prepared. Triggered by ultrasound (US) with high power (>0.5 W cm^-2), VBS-PSA produces a large amount of reactive oxygen species (ROS) through both piezoelectricity-enhanced sonodynamic efficiency and peroxidase-like (POD-like) activity, thereby displaying the powerful antibacterial capability. However, under low-power US (≤0.5 W cm^-2), the piezoelectric effect of VBS-PSA generates electrical signals that significantly stimulate the osteoblast responses (proliferation and osteoblast differentiation) and enhance catalase-like (CAT-like) activity for scavengers of ROS and generation of oxygen, thereby creating a favorable microenvironment for cell growth. Our study presents a novel strategy to apply the piezoelectric effect of hydrogels for enhancing sonodynamic efficiency and nanozyme activities that synergistically kill bacteria and stimulate osteoblast responses. The piezoelectric hydrogel would have great potential for the repair of infected bone defects.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-layered electrode constructs for neural tissue engineering.","authors":"Marjolaine Boulingre, Mateusz Chodkowski, Roberto Portillo Lara, Aaron Lee, Josef Goding, Rylie A Green","doi":"10.1039/d4tb02651a","DOIUrl":"https://doi.org/10.1039/d4tb02651a","url":null,"abstract":"<p><p>Although neural tissue engineering holds great therapeutic potential for multiple clinical applications, one important challenge is the development of scaffolds that provide cues required for neural tissue development. To achieve this, biomaterial systems can be leveraged to present appropriate biological, mechanical, topographical and electrical cues that could direct cell fate. In this study, a multi-layered electrode construct was engineered to be used as a platform for 3D cell encapsulation for <i>in vitro</i> applications. The first layer is a conductive hydrogel coating, that improves electrical conductivity from the underlying platinum electrode. The second layer is a biosynthetic hydrogel, specifically tailored to support neural development. This layered electrode construct was electrochemically characterised, and a numerical model was applied to study electrical stimuli reaching the biosynthetic hydrogel layer. The construct was shown to effectively support the growth and proliferation of encapsulated astrocytes within the biosynthetic layer, while the numerical model will enable computational experimentation for benchmarking and study validation. This highly versatile system represents a robust tool to study the influence of electrical stimuli on neural fate, as well as investigating the development of biohybrid interfaces <i>in vitro</i>.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an AI-derived, non-invasive, label-free 3D-printed microfluidic SERS biosensor platform utilizing Cu@Ag/carbon nanofibers for the detection of salivary biomarkers in mass screening of oral cancer.","authors":"Navami Sunil, Rajesh Unnathpadi, Rajkumar Kottayasamy Seenivasagam, T Abhijith, R Latha, Shina Sheen, Biji Pullithadathil","doi":"10.1039/d4tb02766c","DOIUrl":"https://doi.org/10.1039/d4tb02766c","url":null,"abstract":"<p><p>Developing a non-invasive and reliable tool for the highly sensitive detection of oral cancer is essential for its mass screening and early diagnosis, and improving treatment efficacy. Herein, we utilized a label-free surface enhanced Raman spectroscopy (SERS)-based biosensor composed of Cu@Ag core-shell nanoparticle anchored carbon nanofibers (Cu@Ag/CNFs) for highly sensitive salivary biomarker detection in oral cancer mass screening. This SERS substrate provided a Raman signal enhancement of up to 10⁷ and a detection limit as low as 10^-12 M for rhodamine 6G molecules. Finite-difference time-domain (FDTD) simulation studies on Cu@Ag/CNFs indicated an <i>E</i>-field intensity enhancement factor (|<i>E</i>|²/|<i>E</i>₀|²) of 250 at the plasmonic hotspot induced between two adjacent Cu@Ag nanoparticles. The interaction of this strong <i>E</i>-field along with the chemical enhancement effects was responsible for such huge enhancement in the Raman signals. To realize the real capability of the developed biosensor in practical scenarios, it was further utilized for the detection of oral cancer biomarkers such as nitrate, nitrite, thiocyanate, proteins, and amino acids with a micro-molar concentration in saliva samples. The integration of SERS substrates with a 3D-printed 12-channel microfluidic platform significantly enhanced the reproducibility and statistical robustness of the analytical process. Moreover, AI-driven techniques were employed to improve the diagnostic accuracy in differentiating the salivary profiles of oral cancer patients (<i>n</i>₁ = 56) from those of healthy controls (<i>n</i>₂ = 60). Principal component analysis (PCA) was utilized for dimensionality reduction, followed by classification using a random forest (RF) algorithm, yielding a robust classification accuracy of 87.5%, with a specificity of 92% and sensitivity of 88%. These experimental and theoretical findings emphasize the real-world functionality of the present non-invasive diagnostic tool in paving the way for more accurate and early-stage detection of oral cancer in clinical settings.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unleashing the antibacterial potential of ZIFs and their derivatives: mechanistic insights.","authors":"Geetika Jain, Radhika Chaurasia, Bani Preet Kaur, Ontar Paul Chowdhury, Hiranmay Roy, Richa Rani Gupta, Bhaskar Biswas, Sandip Chakrabarti, Monalisa Mukherjee","doi":"10.1039/d4tb02682a","DOIUrl":"https://doi.org/10.1039/d4tb02682a","url":null,"abstract":"<p><p>Antibiotic resistance presents an alarming threat to global health, with bacterial infections now ranking among the leading causes of mortality. To address this escalating challenge, strategies such as antibiotic stewardship, development of antimicrobial therapies, and exploration of alternative treatment modalities are imperative. Metal-organic frameworks (MOFs), acclaimed for their outstanding biocompatibility and <i>in vivo</i> biodegradability, are promising avenues for the synthesis of novel antibiotic agents under mild conditions. Among these, zeolitic imidazolate frameworks (ZIFs), a remarkable subclass of MOFs, have emerged as potent antibacterial materials; the efficacy of which stems from their porous structure, metal ion content, and tunable functionalized groups. This could be further enhanced by incorporating or encapsulating metal ions, such as Cu, Fe, Ti, Ag, and others. This perspective aims to underscore the potential of ZIFs as antibacterial agents and their underlying mechanisms including the release of metal ions, generation of reactive oxygen species (ROS), disruption of bacterial cell walls, and synergistic interactions with other antibacterial agents. These attributes position ZIFs as promising candidates for advanced applications in combating bacterial infections. Furthermore, we propose a novel approach for synthesizing ZIFs and their derivatives, demonstrating exceptional antibacterial efficacy against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. By highlighting the benefits of ZIFs and their derivatives as antibacterial agents, this perspective emphasizes their potential to address the critical challenge of antibiotic resistance.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A light-driven molecular motor-polypeptide conjugate supports controlled cell uptake.","authors":"Camilla Pegoraro, Ainoa Guinart, Esther Masiá Sanchis, Daniel Doellerer, Marc C A Stuart, Inmaculada Conejos-Sánchez, Ben L Feringa, María J Vicent","doi":"10.1039/d4tb02434f","DOIUrl":"https://doi.org/10.1039/d4tb02434f","url":null,"abstract":"<p><p>While light-driven molecular motors (MMs) hold immense potential to control cell function, low biocompatibility and solubility have hampered their implementation. We developed a novel polypeptide-conjugated MM by linking a propargyl-derivatized light-driven MM to a poly-L-glutamic acid-based carrier (P) with inherent mitochondria tropism through click chemistry, denoted P-MM. P-MM effectively maintained the parental stability and unidirectional rotational capabilities of MM upon irradiation at 405 nm. Light-induced supramolecular conformational changes significantly increased cell uptake compared to non-irradiated controls while retaining the subcellular targeting capacity of P. P-MM exhibited minimal cytotoxicity and reactive oxygen species production, suggesting a non-disruptive interaction with cell membranes. Overall, we establish a connection between irradiation and enhanced biological responses, demonstrating the potential of integrating MMs with targeted polymeric nanocarriers for controlled, light-responsive behavior in biological systems and innovative applications in advanced therapeutic/diagnostic strategies.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemoglobin-loaded ZIF-8 nanoparticles equipped with PEGylated metal-phenolic network coatings: an oxygen carrier with antioxidant and stealth properties.","authors":"Clara Coll-Satue, Eva Cabrera-San Millan, Michelle Maria Theresia Jansman, Lisa Arnholdt, Leticia Hosta-Rigau","doi":"10.1039/d4tb01771d","DOIUrl":"https://doi.org/10.1039/d4tb01771d","url":null,"abstract":"<p><p>Hemoglobin-based oxygen carriers (HBOCs) offer a promising alternative to conventional blood transfusions in emergency scenarios. However, achieving optimal stability, functionality, and biocompatibility in HBOCs remains a significant challenge. In this study, we employed a HBOC formulation consisting of hemoglobin (Hb) encapsulated within zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (NPs). These NPs were subsequently coated with metal phenolic networks (MPNs) and polyethylene glycol (PEG) to impart antioxidant properties and enhance their stability and biocompatibility. Hb-loaded ZIF-8 NPs were synthesized using a rapid, environmentally friendly protocol and exhibited desirable properties, including an average size of approximately 150 nm, a negative surface charge (zeta potential of -14 mV), high encapsulation efficiency (approximately 65%), and substantial drug loading capacity (around 70%). The MPN coating significantly enhanced stability across various buffers and cell media and endowed the NPs with antioxidant properties. Meanwhile, the PEG layer conferred stealth properties, potentially extending circulation times <i>in vivo</i>. Furthermore, the NPs showed excellent biocompatibility in terms of cell viability and hemolysis rate studies. They also efficiently bound and released oxygen across multiple cycles, demonstrating preserved functionality. These attributes highlight the potential of our novel HBOC as an effective oxygen delivery system and position our formulation as a promising candidate for clinical application in critical care, providing a strategic alternative to traditional blood transfusions.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellulose-based bioactive material and turmeric-impregnated flexible and biocompatible scaffold for bone tissue engineering applications.","authors":"Shital S Shendage, Kranti Kachare, Kajal Gaikwad, Shivaji Kashte, Fu-Der Mai, Anil Vithal Ghule","doi":"10.1039/d4tb02028f","DOIUrl":"https://doi.org/10.1039/d4tb02028f","url":null,"abstract":"<p><p>Metal transplants, autografts, and allografts are currently used for the treatment of bone-related problems, but each comes with inherent limitations. However, advances in science and technology have underscored the need for the development of cost-effective, eco-friendly, and customized architectural scaffolds with desirable porosity and mechanical strength. Additionally, the synthesis of sustainable scaffolds using biowaste is being studied to decrease environmental pollution. Green fabrication of scaffolds has an inestimable influence on decreasing production costs and toxicity while increasing biological compatibility. With this motivation, in the present study, a 70S30C calcium silicate bioactive material (BM) was synthesized through a simple precipitation method, using recycled rice husk (as a silica source) and eggshells (as a calcium source). Further, the BM and turmeric powder (Tm) were impregnated onto cellulose-based cotton fabric (CF), considering its easy availability, flexibility, mechanical strength, and cost-effective nature. The prepared scaffolds were characterized using UV-visible spectroscopy, XRD, FTIR spectroscopy, SEM, and EDS mapping. Further, <i>in vitro</i> bioactivity and degradation studies were performed in simulated body fluid (SBF). The <i>in vitro</i> haemolysis study revealed less than 5% haemolysis. <i>Ex ovo</i> CAM results showed good neovascularization. Both <i>in vitro</i> and <i>in vivo</i> biocompatibility studies demonstrated non-toxic nature. Furthermore, <i>in vivo</i> osteogenesis results showed bone regeneration capacity, as confirmed by X-ray and histological analysis. Thus, the CF template impregnated with BM and Tm acts as a porous, flexible, bioactive, degradable, haemocompatible, osteogenic, antibacterial, cost-effective, and eco-friendly scaffold for bone tissue engineering applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1,4-Bis(2-hydroxyethyl)piperazine-derived water-dispersible and antibacterial polyurethane coatings for medical catheters.","authors":"Anchal Gupta, Simran Kaur Rainu, Manleen Kaur, Mahipal Meena, Neetu Singh, Josemon Jacob","doi":"10.1039/d4tb02227k","DOIUrl":"https://doi.org/10.1039/d4tb02227k","url":null,"abstract":"<p><p>To prolong usage and mitigate infections associated with bacterial colonization on medical catheters, the development of water-dispersible polyurethane (PU) coatings with bactericidal properties is desirable. With this objective, we have formulated polyurethane coatings that exhibit both antibacterial activity and water dispersibility. A piperazine-based diol, 1,4-bis(2-hydroxyethyl)piperazine (HEPZ), was synthesized and used as a chain extender in PU synthesis. The PUs were prepared using hexamethylene diisocyanate (HDI), 4,4'-methylene diphenyl diisocyanate (MDI), polyethylene glycol (PEG₆₀₀), and polypropylene glycol (PPG₄₀₀), resulting in a series of polyurethanes (PU1-PU4). MDI-containing PUs showed superior tensile strength (3.2-3.6 MPa) and elongation (67-70%) attributable to their higher aromatic content. The PEG₆₀₀-containing PUs (PU1 and PU3) were alkylated using methyl iodide (MeI) to varying degrees whereby a significant reduction in contact angle from ∼82° to ∼62° was observed, indicating enhanced hydrophilicity. MPU3-D with 72.5% methylation demonstrated the most stable water dispersion with a particle size of ∼190.8 nm and a zeta potential of +49.0 mV. <i>In vitro</i> cytocompatibility studies further revealed that methylated PU3 exhibited higher compatibility (80-90%) compared to methylated PU1 (30-40%). The hemolysis test showed the non-hemolytic behavior of MPU3-D films with a % hemolysis of 0.4 ± (0.2)% making it suitable for coating on medical devices. Additionally, MPU3-D films also demonstrated antibacterial activity against Gram-negative (<i>E. coli</i>) and Gram-positive (<i>S. aureus</i>) bacteria, with zones of inhibition measuring 7 mm and 8 mm, respectively. Also, water-dispersible MPU3-D-based coatings with a hardness of ∼75 A and a thickness of ∼17 μm (as observed through FESEM) showed strong adhesion to PVC catheters, exhibiting an adhesion strength of 4B rating. Our results suggest that water-dispersible polyurethane coatings with antibacterial properties are promising materials to reduce catheter-associated infections and enhance patient care.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A non-hydrolysable peptidomimetic for mitochondrial targeting.","authors":"Yeray Folgar-Cameán, Daniel Torralba-Maldonado, Patricia Fulias-Guzmán, Marta Pazo, Irene Máximo-Moreno, Miriam Royo, Ona Illa, Javier Montenegro","doi":"10.1039/d4tb01626b","DOIUrl":"https://doi.org/10.1039/d4tb01626b","url":null,"abstract":"<p><p>Peptidomimetics, molecules that mimic the activity of natural peptides with improved stability or bioavailability, have emerged as interesting materials with applications in biomedicine. In this study, we describe a hybrid γ,γ-peptidomimetic that efficiently aims at mitochondria, a key therapeutic target associated with several disorders, in living cells. Peptide backbones with a component of cationic and hydrophobic amino acids have been shown to preferentially target mitochondria due to their high negative membrane potential and hydrophobic character of the membranous invaginations of these key organelles. We here exploit the advantageous bioorthogonal properties of a peptidomimetic scaffold that consists of an alternation of (1<i>S</i>,3<i>R</i>)-3-amino-2,2-dimethylcyclobutane-1-carboxylic acid and an <i>N</i>^α-functionalised <i>cis</i>-γ-amino-L-proline derivative. This peptidomimetic exhibited excellent membrane translocation efficiency, mitochondrial targeting ability, and biocompatibility. Mitochondrial targeting was confirmed to be dependent on the electrochemical potential generated by the electron transport chain. The presence of non-natural amino acids rendered the compound exceptionally stable in the presence of proteases, maintaining its integrity and functionality for targeting the organelle even after 1 week of incubation in serum. This stability, coupled with its targeting abilities and the low cytosolic/endosomal residual signal, facilitated the tracking of relevant mitochondrial dynamics, including fission events and intracellular movement. Additionally, this peptidomimetic scaffold allowed the sustained and precise mitochondrial targeting of a pH sensitive ratiometric probe, 5(6)-carboxy-SNARF-1, which enabled mitochondrial pH monitoring. In summary, our study introduces a biomimetic peptide with exceptional mitochondria-targeting properties, ensuring stability in biological media and offering insights into crucial mitochondrial processes.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}