Journal of materials chemistry. B最新文献

{"title":"FRET-based mesoporous organosilica nanoplatforms for <i>in vitro</i> and <i>in vivo</i> anticancer two-photon photodynamic therapy.","authors":"Nicolas Bondon, Clément Charlot, Lamiaa M A Ali, Alexandre Barras, Nicolas Richy, Denis Durand, Yann Molard, Grégory Taupier, Erwan Oliviero, Magali Gary-Bobo, Frédéric Paul, Sabine Szunerits, Nadir Bettache, Jean-Olivier Durand, Christophe Nguyen, Rabah Boukherroub, Olivier Mongin, Clarence Charnay","doi":"10.1039/d4tb02103g","DOIUrl":"https://doi.org/10.1039/d4tb02103g","url":null,"abstract":"<p><p>We report the synthesis of multifunctional periodic mesoporous organosilica nanoparticles (PMO NPs) with substantial two-photon absorption properties and targeting capability for two-photon excitation fluorescence (TPEF) and photodynamic therapy (TPE-PDT). Prepared using an adapted sol-gel synthesis, the nanoplatforms integrated two silylated chromophores in their three-dimensional matrix to maximize non-radiative Förster resonance energy transfer from a high two-photon absorption fluorophore donor to a porphyrin derivative acceptor, leading to an enhanced generation of reactive oxygen species. Combinations of biodegradable and non-biodegradable bis(triethoxysilyl)alkoxysilanes were employed for the synthesis of the NPs, and the corresponding photophysical studies revealed high efficiency levels of FRET. Next, the cellular uptake and toxicities of pristine and functionalized NPs were evaluated on breast cancer cell lines upon TPEF and TPE-PDT. Notably, the use of TPE-PDT treatment led to high levels of phototoxicity on MCF-7 and MDA-MB-231 cancer cells with substantial effects when compared to one-photon excitation (OPE)-PDT treatment. Preliminary <i>in vivo</i> data on selective and biodegradable NPs showed a significant phototoxicity towards MDA-MB-231 on zebrafish xenograft embryos, making these advanced nanoplatforms promising candidates for future TPE-PDT-based cancer treatments.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-valent polymyxin-based fluorescent probe for the detection of Gram-negative infections.","authors":"Richa Sharma, Maria Rodriguez-Rios, James Crossland, Maulida Septiyana, Alicia Megia-Fernandez, Maxime Klausen, Mark Bradley","doi":"10.1039/d4tb01786b","DOIUrl":"https://doi.org/10.1039/d4tb01786b","url":null,"abstract":"<p><p>A multi-branched fluorogenic probe for the rapid and specific detection of Gram-negative bacteria is reported. Three Gram-negative-targeting azido-modified polymyxins were clicked onto a trivalent scaffold functionalised with the environmental green-emitting fluorophore 7-nitrobenz-2-oxa-1,3-diazole. The probe allowed wash-free detection of target bacteria with increased sensitivity and lower limits of detection compared to monovalent probes.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, synthesis, and characterization of an Ag-Bi-S-based multifunctional nanotheranostic platform.","authors":"Yuan-Yi Tan, Xin Chen, Dong-Yun Zheng, Chao Liu, Xiao-Jun Liu, Xian-Guang Lin, Chun-Nan Zhu","doi":"10.1039/d4tb02502d","DOIUrl":"https://doi.org/10.1039/d4tb02502d","url":null,"abstract":"<p><p>This paper reports an Ag-Bi-S-based nanotheranostic platform with an ingeniously designed heterostructure, an appropriate size, and good imaging and therapy performances. By comparing the fluorescence property and Bi element content, the optimal heterostructure was demonstrated to be Ag₂S/Bi₂S₃ core/shell. The hydrophilic Ag₂S/Bi₂S₃-PEG nanocrystals with hydrodynamic diameter of 37.56 nm exhibited near-infrared-II fluorescence, good CT imaging contrast, and a high photothermal conversion efficiency (38.4%), and have shown significant potential in the precision diagnosis and treatment of tumors.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging bioengineering breakthroughs in precision diagnosis and therapy for endometriosis and adenomyosis.","authors":"Yujie Peng, Meng Zhang, Jingjing Yan, Rong Wang, Yu Xin, Xiaoling Zheng, Libo Zhu, Weidong Fei, Mengdan Zhao","doi":"10.1039/d4tb01755b","DOIUrl":"https://doi.org/10.1039/d4tb01755b","url":null,"abstract":"<p><p>Endometriosis and adenomyosis are debilitating gynecological conditions that severely affect the quality of life of women. Traditional diagnostic and treatment methods, including laparoscopic surgery and hormonal therapy, face significant limitations such as incomplete lesion detection, high recurrence rates, and adverse side effects. Emerging bioengineering technologies offer promising solutions for precise diagnosis and therapy of these diseases. Advances in biomarker detection through electrochemical immunosensors, including specific molecular markers like cytokines and growth factors, have improved their early diagnosis. Innovative imaging techniques, such as near-infrared fluorescence imaging, magnetic resonance imaging, and photoacoustic imaging, enhance lesion visualization and surgical precision. In therapeutic applications, bioengineered drug delivery systems enable targeted therapy by modifying drug carriers with ligands targeting highly expressed receptors in endometriotic lesions. Such strategies could improve drug accumulation at target sites and reduce damage to healthy tissues. Integrating external energy (including lasers, focused ultrasound, and magnetic fields) with nanoplatforms offers key benefits for treating endometriosis and adenomyosis, allowing precise delivery of energy-responsive molecules to lesions and minimizing damage to healthy tissues. Additionally, novel approaches, such as immunotherapy, gene therapy, ferroptosis induction, and synthetic lethal activation, offer new avenues for effective treatment of endometriosis and adenomyosis. Significantly, this paper discusses the advantages of precision diagnosis and treatment of endometriosis in preserving the fertility of women of reproductive age. This review highlights the potential of bioengineering breakthroughs to transform the diagnosis and management of endometriosis and adenomyosis, emphasizing their role in advancing precision medicine and improving women's health.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct covalent attachment of fluorescent molecules on plasma polymerized nanoparticles: a simplified approach for biomedical applications.","authors":"Laura Libnan Haidar, Yuheng Wang, Aaron D Gilmour, Elmer Austria, Badwi B Boumelhem, Naveed Aziz Khan, Arifah Anwar Fadzil, Stuart T Fraser, Marcela M M Bilek, Behnam Akhavan","doi":"10.1039/d4tb01515k","DOIUrl":"https://doi.org/10.1039/d4tb01515k","url":null,"abstract":"<p><p>Polymeric nanoparticles surface functionalised with fluorescent molecules hold significant potential for advancing diagnostics and therapeutic delivery. Despite their promise, challenges persist in achieving robust attachment of fluorescent molecules for real-time tracking. Weak physical adsorption, pH-dependent electrostatic capture, and hydrophobic interactions often fail to achieve stable attachment of fluorescent markers. While covalent attachment offers stability, it often entails laborious multi-step wet-chemistry processes. This work demonstrates that plasma polymerised nanoparticles (PPNs) can directly and covalently attach fluorescent molecules with no need for additional interim treatment processes. For the first time, we provide evidence indicating the formation of covalent bonds between the fluorescent molecules and PPN surfaces. Two model fluorescent molecules, fluorescein isothiocyanate (FITC) and Nile blue (NB), were attached to PPNs in a one-step process. The attached molecules remained on nanoparticle surfaces even after detergent washing, as confirmed by a combination of X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy, flow cytometry, and time-of-flight secondary ion mass spectrometry (ToF-SIMS) data. The robust attachment of fluorescent molecules on PPNs ensures their stability and functionality, enhancing the potential of these fluorescently labelled nanoparticles for diagnostic, therapeutic, and imaging applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon oxide microchips functionalized with fluorescent probes for quantitative real-time glutathione sensing in living cells.","authors":"Saman Bagherpour, Patricia Vázquez, Marta Duch, Juan Pablo Agusil, José Antonio Plaza, Mariano Redondo-Horcajo, Teresa Suárez, Lluïsa Pérez-García","doi":"10.1039/d4tb01859a","DOIUrl":"https://doi.org/10.1039/d4tb01859a","url":null,"abstract":"<p><p>Glutathione (GSH) plays a vital role in the regulation of intracellular functions which alterations in physiological glutathione levels are associated to various diseases. Molecular bioimaging is a sensitive method for GSH detection, but challenges persist in the development of fluorescent probes, mainly concerning long-term tracking of intracellular GSH concentration because of aggregation of molecular probes and their washout in cells. Engineered nanomaterials have shown great promise for increasing the disease diagnosis accuracy. Microchips generated by advanced microfabrication techniques can be applied in designing biomedical devices due to control over size, shape, and bioactive coatings utilization. In the current work, the synthesis and characterization of two GSH probes, Bdpy1 and Bdpy2, is reported, each offering irreversible and reversible GSH reactions, respectively. These GSH probes are immobilized on silicon oxide microchips (SOμC), micro-fabricated using photolithographic techniques, to give SOμC-Bdpy1 and SOμC-Bdpy2. Both functionalized microchips exhibited sensitivity to GSH, and, notably, the reversible SOμC-Bdpy2 showed less time dependency, making it more suitable for long-term intracellular GSH sensing. <i>In vitro</i> experiments in HeLa cells reveal both SOμC-Bdpy1 and SOμC-Bdpy2 were internalized in living cells, showing SOμC-Bdpy2 more reliable results (due to its less time dependency) for quantifying intracellular GSH. Remarkably, the intracellular GSH measurement was monitored by SOμC-Bdpy2 for 48 h, indicating the functionalized microchips capability to detect GSH amount in different time intervals. This study introduces a promising approach for long term quantification of intracellular GSH, overcoming the limitation of fluorescent probes and offering valuable insights into microchip-based sensing methodologies.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A step towards non-invasive diagnosis of diabetes mellitus using <i>in situ</i> synthesized MOF-MXene hybrid material with extended gate field-effect transistor integration.","authors":"Mallikarjuna Swamy Shabanur Matada, Rahul Suresh Ghuge, Surya Velappa Jayaraman, Corrado Di Natale, Yuvaraj Sivalingam","doi":"10.1039/d4tb01866d","DOIUrl":"https://doi.org/10.1039/d4tb01866d","url":null,"abstract":"<p><p>The increasing demand for non-invasive and non-enzymatic glucose sensors is driven by the objective of eliminating the need for blood pricks from the body and enabling enzyme-free detection of glucose for diagnosing diabetes mellitus. To address this need, we synthesized Ni MOF-MXene (Ni_BDC-MXene) hybrid material through a one-pot synthesis method, which acts as a catalyst to detect salivary glucose using an extended gate field effect transistor (EGFET) method. The resulting sensor exhibits good selectivity towards glucose over common interfering molecules such as sucrose, fructose, maltose, uric acid, and ascorbic acid under physiological conditions in saliva. The fabricated electrode demonstrated high sensitivity of 531.78 μA mM^-1 cm^-2 with a detection range of 10 μM to 1100 μM, a sensor response time of less than 5 s, and a limit of detection (LOD) of 0.29 μM. The real saliva sample measurements under postabsorptive and postprandial conditions highlight the electrode's effectiveness in detecting salivary glucose. In addition to EGFET measurements, scanning Kelvin probe (SKP) measurements were performed to understand the mechanism of charge transfer between the glucose and Ni_BDC-MXene/CP electrode. Overall, the EGFET results demonstrate the capability of the sensor to detect salivary glucose in hypoglycemia, normal, and hyperglycemia ranges.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In situ</i> developed NiCo₂O₄-Ti₃C₂T_<i>x</i> nanohybrid towards non-enzymatic electrochemical detection of glucose and hydrogen peroxide.","authors":"Devarasu Mohanapriya, Kathavarayan Thenmozhi","doi":"10.1039/d4tb02265c","DOIUrl":"https://doi.org/10.1039/d4tb02265c","url":null,"abstract":"<p><p>Owing to the adverse consequences of excess glucose (Glu) and hydrogen peroxide (H₂O₂) on humans, it is imperative to develop an electrochemical sensor for detection of these analytes with good selectivity and sensitivity. Herein, a nanohybrid comprising nickel cobaltite nanoparticles (NiCo₂O₄ NPs) embedded on conductive Ti₃C₂T_<i>x</i> nanosheets (NSs) has been prudently designed and employed for the electrochemical detection of Glu and H₂O₂. The developed nanohybrid has been systematically characterized using morphological and spectral techniques, and then immobilized on a glassy carbon electrode (GCE). Under optimized conditions, the developed NiCo₂O₄-Ti₃C₂T_<i>x</i>/GCE based electrochemical sensor has demonstrated an impressive analytical response towards Glu and H₂O₂ with good sensitivity and selectivity. The non-enzymatic sensor has demonstrated a broad linear range from 30 μM to 1.83 mM for Glu, and two linear ranges of 20-100 μM and 100 μM-2.01 mM for H₂O₂. The sensor has exhibited limits of detection (LOD) of 9 μM and 6 μM with sensitivities of 101.2 μA μM^-1 cm^-2 and 107.03 μA μM^-1 cm^-2, respectively, for Glu and H₂O₂ detection. The impressive analytical performance of the fabricated sensor in terms of linear range, LOD and sensitivity are ascribed to the (i) enhanced conductivity of Ti₃C₂T_<i>x</i> NSs, (ii) mediated electrocatalytic activity of NiCo₂O₄ NPs and (iii) large number of catalytically active sites on the NiCo₂O₄-Ti₃C₂T_<i>x</i> heterostructure. Notably, the NiCo₂O₄-Ti₃C₂T_<i>x</i>/GCE has demonstrated impressive stability and reproducibility, which is mainly due to the <i>in situ</i> uniform growth of NiCo₂O₄ NPs over Ti₃C₂T_<i>x</i> NSs.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-derived biomaterials for targeted drug delivery and tissue repair.","authors":"Xinyu Fang, Ya Li, Yulin Wang, Rupeng Cai, Qiang Ao","doi":"10.1039/d4tb02477j","DOIUrl":"https://doi.org/10.1039/d4tb02477j","url":null,"abstract":"<p><p>Platelets are nucleic-free cells with a lifespan of 7-10 days in the bloodstream, playing a crucial role in various physiological processes such as hemostasis, thrombus formation, tumor development and metastasis, inflammation, and host defense. By utilizing the unique structural and functional characteristics of platelets, platelet-modified nano-drugs can evade immune recognition and clearance and facilitate prolonged circulation <i>in vivo</i>, which ultimately allows the nanoparticles to reach sites of disease such as thrombi, tumors, inflammation, or bacterial infections, leading to specific adhesion and significantly enhancing the efficiency of targeted drug delivery. This paper reviews the novel design and application of platelet-derived biomaterials in various diseases in recent years and comprehensively demonstrates the potential of platelet-derived biomaterials in the fields of disease therapy and biodefence, which will provide a reference for advancing the development of platelet-derived biomaterials and clinical practice.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin nanocrystal-loaded alginate hydrogel patch for wound healing applications.","authors":"Malay Nayak, Vivek Kumar, Durba Banerjee, Lipi Pradhan, Prajwal Kamath, Sudip Mukherjee","doi":"10.1039/d4tb01699h","DOIUrl":"https://doi.org/10.1039/d4tb01699h","url":null,"abstract":"<p><p>Wound healing can often be delayed due to non-favourable physiological conditions. Current treatment strategies have many limitations, and the development of novel therapeutic patches is urgently required. Herein, we have developed a hydrogel-based wound healing patch containing quercetin nanocrystals to enhance quercetin solubility, leading to sustained release and improved bioactivity. Due to the anti-oxidant properties of quercetin, a sustained release of the drug is highly beneficial for the rapid repair of wounds by reducing oxidative stress. Quercetin nanocrystals with a size of 600-800 nm were synthesized that demonstrated sustained release of quercetin when fabricated in a hydrogel patch. This has been utilized for <i>in vivo</i> wound repair in rat and mouse models of skin wounds. Overall, our study demonstrates the usability of a novel therapeutic hydrogel patch containing phytochemical-based nanocrystals for rapid wound healing applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142879152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}