Journal of materials chemistry. B最新文献

{"title":"Cellulose-based bioactive material and turmeric-impregnated flexible and biocompatible scaffold for bone tissue engineering applications.","authors":"Shital S Shendage, Kranti Kachare, Kajal Gaikwad, Shivaji Kashte, Fu-Der Mai, Anil Vithal Ghule","doi":"10.1039/d4tb02028f","DOIUrl":"https://doi.org/10.1039/d4tb02028f","url":null,"abstract":"<p><p>Metal transplants, autografts, and allografts are currently used for the treatment of bone-related problems, but each comes with inherent limitations. However, advances in science and technology have underscored the need for the development of cost-effective, eco-friendly, and customized architectural scaffolds with desirable porosity and mechanical strength. Additionally, the synthesis of sustainable scaffolds using biowaste is being studied to decrease environmental pollution. Green fabrication of scaffolds has an inestimable influence on decreasing production costs and toxicity while increasing biological compatibility. With this motivation, in the present study, a 70S30C calcium silicate bioactive material (BM) was synthesized through a simple precipitation method, using recycled rice husk (as a silica source) and eggshells (as a calcium source). Further, the BM and turmeric powder (Tm) were impregnated onto cellulose-based cotton fabric (CF), considering its easy availability, flexibility, mechanical strength, and cost-effective nature. The prepared scaffolds were characterized using UV-visible spectroscopy, XRD, FTIR spectroscopy, SEM, and EDS mapping. Further, <i>in vitro</i> bioactivity and degradation studies were performed in simulated body fluid (SBF). The <i>in vitro</i> haemolysis study revealed less than 5% haemolysis. <i>Ex ovo</i> CAM results showed good neovascularization. Both <i>in vitro</i> and <i>in vivo</i> biocompatibility studies demonstrated non-toxic nature. Furthermore, <i>in vivo</i> osteogenesis results showed bone regeneration capacity, as confirmed by X-ray and histological analysis. Thus, the CF template impregnated with BM and Tm acts as a porous, flexible, bioactive, degradable, haemocompatible, osteogenic, antibacterial, cost-effective, and eco-friendly scaffold for bone tissue engineering applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1,4-Bis(2-hydroxyethyl)piperazine-derived water-dispersible and antibacterial polyurethane coatings for medical catheters.","authors":"Anchal Gupta, Simran Kaur Rainu, Manleen Kaur, Mahipal Meena, Neetu Singh, Josemon Jacob","doi":"10.1039/d4tb02227k","DOIUrl":"https://doi.org/10.1039/d4tb02227k","url":null,"abstract":"<p><p>To prolong usage and mitigate infections associated with bacterial colonization on medical catheters, the development of water-dispersible polyurethane (PU) coatings with bactericidal properties is desirable. With this objective, we have formulated polyurethane coatings that exhibit both antibacterial activity and water dispersibility. A piperazine-based diol, 1,4-bis(2-hydroxyethyl)piperazine (HEPZ), was synthesized and used as a chain extender in PU synthesis. The PUs were prepared using hexamethylene diisocyanate (HDI), 4,4'-methylene diphenyl diisocyanate (MDI), polyethylene glycol (PEG₆₀₀), and polypropylene glycol (PPG₄₀₀), resulting in a series of polyurethanes (PU1-PU4). MDI-containing PUs showed superior tensile strength (3.2-3.6 MPa) and elongation (67-70%) attributable to their higher aromatic content. The PEG₆₀₀-containing PUs (PU1 and PU3) were alkylated using methyl iodide (MeI) to varying degrees whereby a significant reduction in contact angle from ∼82° to ∼62° was observed, indicating enhanced hydrophilicity. MPU3-D with 72.5% methylation demonstrated the most stable water dispersion with a particle size of ∼190.8 nm and a zeta potential of +49.0 mV. <i>In vitro</i> cytocompatibility studies further revealed that methylated PU3 exhibited higher compatibility (80-90%) compared to methylated PU1 (30-40%). The hemolysis test showed the non-hemolytic behavior of MPU3-D films with a % hemolysis of 0.4 ± (0.2)% making it suitable for coating on medical devices. Additionally, MPU3-D films also demonstrated antibacterial activity against Gram-negative (<i>E. coli</i>) and Gram-positive (<i>S. aureus</i>) bacteria, with zones of inhibition measuring 7 mm and 8 mm, respectively. Also, water-dispersible MPU3-D-based coatings with a hardness of ∼75 A and a thickness of ∼17 μm (as observed through FESEM) showed strong adhesion to PVC catheters, exhibiting an adhesion strength of 4B rating. Our results suggest that water-dispersible polyurethane coatings with antibacterial properties are promising materials to reduce catheter-associated infections and enhance patient care.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A non-hydrolysable peptidomimetic for mitochondrial targeting.","authors":"Yeray Folgar-Cameán, Daniel Torralba-Maldonado, Patricia Fulias-Guzmán, Marta Pazo, Irene Máximo-Moreno, Miriam Royo, Ona Illa, Javier Montenegro","doi":"10.1039/d4tb01626b","DOIUrl":"10.1039/d4tb01626b","url":null,"abstract":"<p><p>Peptidomimetics, molecules that mimic the activity of natural peptides with improved stability or bioavailability, have emerged as interesting materials with applications in biomedicine. In this study, we describe a hybrid γ,γ-peptidomimetic that efficiently aims at mitochondria, a key therapeutic target associated with several disorders, in living cells. Peptide backbones with a component of cationic and hydrophobic amino acids have been shown to preferentially target mitochondria due to their high negative membrane potential and hydrophobic character of the membranous invaginations of these key organelles. We here exploit the advantageous bioorthogonal properties of a peptidomimetic scaffold that consists of an alternation of (1<i>S</i>,3<i>R</i>)-3-amino-2,2-dimethylcyclobutane-1-carboxylic acid and an <i>N</i>^α-functionalised <i>cis</i>-γ-amino-L-proline derivative. This peptidomimetic exhibited excellent membrane translocation efficiency, mitochondrial targeting ability, and biocompatibility. Mitochondrial targeting was confirmed to be dependent on the electrochemical potential generated by the electron transport chain. The presence of non-natural amino acids rendered the compound exceptionally stable in the presence of proteases, maintaining its integrity and functionality for targeting the organelle even after 1 week of incubation in serum. This stability, coupled with its targeting abilities and the low cytosolic/endosomal residual signal, facilitated the tracking of relevant mitochondrial dynamics, including fission events and intracellular movement. Additionally, this peptidomimetic scaffold allowed the sustained and precise mitochondrial targeting of a pH sensitive ratiometric probe, 5(6)-carboxy-SNARF-1, which enabled mitochondrial pH monitoring. In summary, our study introduces a biomimetic peptide with exceptional mitochondria-targeting properties, ensuring stability in biological media and offering insights into crucial mitochondrial processes.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting and structural engineering of light-responsive nanoprobes for hierarchical therapy: construction, optimization, and applications in cancer stem cells.","authors":"Jiangluqi Song, Jinhang Hu, Huan Li, Pei Xiang, Zhiqiang Wang, Xiaofang Wang, Shuxia Qi, Mingya Yang, Lixin Zhu","doi":"10.1039/d4tb02508c","DOIUrl":"https://doi.org/10.1039/d4tb02508c","url":null,"abstract":"<p><p>Cancer stem cells (CSCs) possess the ability to self-renew and exhibit high differentiation potential, and they have been proven to be responsible for the maintenance, growth, and metastasis of tumors. As such, accurate identification and targeted therapy for CSCs are of great importance in clinical treatment. Here, a dual-targeted and light-responsive nanoprobe is presented, utilizing the reconstructed mesoporous SiO₂ of a binary fatty acid eutectic mixture and a gold porous shell. The gold shell in the nanoprobe sustains a large absorption cross-section, providing a robust photothermal treatment effect against CSCs upon NIR irradiation. The photothermal effect simultaneously melts the eutectic mixture, which acted as the gating material, triggering the release of nuclear-targeted photosensitizers for photodynamic therapy (PDT). Additionally, to improve the hypoxic environment during PDT, hemoglobin (Hb) is conjugated to the nanoprobe using disulfide as a cross-linker, which can consume cellular glutathione while releasing Hb to deliver oxygen for PDT. Under the synergistic effect of photothermal therapy (PTT) and PDT, cytoplasmic organelles and intranuclear genetic materials are hierarchically damaged, initiating a cascade of reactions, including evident endoplasmic reticulum stress and inflammation. These responses, in turn, promote stem cell death and inhibit tumorigenicity. Furthermore, machine learning models, including random forest (FR), CatBoost, XGBoost, and LightGBM, were employed to optimize the reaction conditions for maximizing the synergistic effect, with CatBoost achieving the best performance. Additionally, antibody-conjugated nanoprobes effectively targeted colon cancer stem cells, demonstrating enhanced phototoxicity and the potential to suppress tumorsphere formation. Therefore, this dual-targeting nanoprobe demonstrates outstanding therapeutic integration performance and shows promise as a platform for synergistic PTT/PDT therapy of CSCs.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-photostable fluorescent dye molecular engineering-for measuring plant cells' membrane-spacing through a \"deposition-embedding\" strategy.","authors":"Wendong Jin, Jie Huang, Jie Niu, Shiqian Zhang, Zhiqiang Liu, Xiaoqiang Yu","doi":"10.1039/d4tb02546f","DOIUrl":"https://doi.org/10.1039/d4tb02546f","url":null,"abstract":"<p><p>The plant cell membrane serves as a barrier, isolating the cell's interior from its external environment. Unlike animal cells, where the cytoplasmic membrane can be easily fluorescently labeled through genetic engineering, plant cells often rely more heavily on small molecule fluorescent probes to address the problem of probe internalization. Meanwhile, due to cellular internalization, current plasma fluorescent probes struggle to stain cell membranes for long periods of time. In addition, these probes tend to accumulate in the cell wall, making it impossible to achieve specific, high-noise-to-noise staining of cell membranes. In response to these challenges, we propose a novel \"deposition-embedding\" strategy for developing a plant cell membrane probe. The compound PTBT-O-NPh2, with its low solubility and high hydrophobicity, is designed to limit membrane penetration. Instead, it rapidly deposits on the membrane surface and embeds itself into the lipid environment <i>via</i> strong hydrogen bonding with phospholipid molecules. Additionally, its exceptional resistance to photobleaching and long-term retention capability allow it to measure membrane-spacing over a period of 120 hours. These findings suggest that the \"deposition-embedding\" strategy could be instrumental in developing a new generation of fluorescent dyes for studying plant mechanobiology.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-functionalized nanocapsules for targeted inhibition of β2-microglobulin amyloid aggregation.","authors":"Lin Tang, Miao Sun, Junnan Chen, Qiong Dai, Song Xue, Chaoyong Liu, Ming Zhang","doi":"10.1039/d4tb01347f","DOIUrl":"https://doi.org/10.1039/d4tb01347f","url":null,"abstract":"<p><p>Dialysis-related amyloidosis (DRA) is a severe complication in patients undergoing long-term dialysis, primarily driven by the deposition of β2-microglobulin (β2m) amyloid fibrils. The effective sequestration and removal of β2m from the bloodstream represent key therapeutic strategies for managing DRA. In this study, we developed a β2m-binding peptide (KDWSFYILAHTEF, denoted as CF)-functionalized nanocomposite (NC-CF), consisting of a protein nanocapsule surface modified with CF peptides to enable specific β2m binding. NC-CF effectively modulates β2m aggregation, transforming slender fibrils into larger clumps while providing steric hindrance to prevent further aggregation. With a high adsorption capacity, 1 μg of NC-CF can adsorb approximately 1 μg of β2m during dialysis, highlighting its potential as an efficient adsorbent for <i>in vitro</i> β2m removal. Furthermore, NC-CF exhibits excellent biocompatibility and significantly mitigates β2m aggregate-induced cytotoxicity, achieving a cell protection rate exceeding 70%. These findings suggest that NC-CF holds great promise as a cytoprotective agent and a nanoinhibitor of β2m aggregation <i>in vivo</i>. Overall, NC-CF offers a novel and effective approach for alleviating DRA by simultaneously removing β2m and safeguarding cells against amyloid-induced toxicity.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luminescent bio-sensors <i>via</i> co-assembly of hen egg white lysozyme with Eu³⁺/Tb³⁺-complexes.","authors":"Min Zhang, Miao Qiu, Zengkun Li, Rui Xu, Yao Wang, Wei Wang, Christopher D Snow, Matt J Kipper, Laurence A Belfiore, Jianguo Tang","doi":"10.1039/d4tb01766h","DOIUrl":"https://doi.org/10.1039/d4tb01766h","url":null,"abstract":"<p><p>Protein crystals have advantageous properties as framework materials, such as porosity and organized, high-density functional groups with the potential for guest specificity. Thus, protein crystal materials open up vast opportunities for fluorescent species doping and drug sensing. In this work, we explore this frontier by combining two lanthanide complexes with hen egg white lysozyme (HEWL) and directly obtaining co deposited structures in one step using an anti-solvent method different from the previous two-step method. Cross-linking of the protein was achieved using glutaraldehyde, ensuring the stability of the assembly in diverse solvent environments. The use of glutaraldehyde achieved protein cross-linking, ensuring the stability of the components in various solvent environments, including no leakage of fluorescent substances in ultrapure water and anhydrous ethanol. Differential fluorescence quenching effects of amino acids on the two doped luminescent complexes were observed. Introduction of amino acids, varying in concentration and type, resulted in distinct fluorescence enhancement or quenching effects on the protein assembly loaded with the complexes, and the detection results are reflected through different fitting equations and parameters. By exploring the application of this hybrid material for amino acid detection, this work lays the groundwork for broader applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citrus pectin-coated inhalable PLGA nanoparticles for treatment of pulmonary fibrosis.","authors":"Kalindu Perera, Moez Ghumman, Parand Sorkhdini, Carmelissa Norbrun, Seraphina Negash, Yang Zhou, Jyothi U Menon","doi":"10.1039/d4tb01682c","DOIUrl":"10.1039/d4tb01682c","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a chronic interstitial disorder of the respiratory system that can be debilitating as it progresses and has experienced a slow rise in incidence in past years. Treatment is complicated by the complex aetiology of the disease and the off-target effects of the two FDA-approved therapeutics available on the market: pirfenidone and nintedanib. In this work, we propose a multipurpose nanoparticle system consisting of poly(lactic-<i>co</i>-glycolic) acid polymer (PLGA) and a coating of citrus pectin (CP) for galectin-3 targeting and anti-fibrotic therapy. Pectin from citrus peels has been observed to have anti-fibrotic activity in a range of fibrotic tissues, causing a decrease in the expression and activity of galectin-3: a key, upregulated marker of fibrosis. We show that the CP-PLGA nanoparticles (NPs) have an average diameter of 340.5 ± 10.6 nm, compatible with inhalation and retention in the deep lung, and that CP constitutes, on average, 40.3% of the final CP-PLGA formulation. The NPs are well-tolerated by MRC-5 lung fibroblasts up to 2 mg mL^-1. We demonstrate the NPs' ability to target transforming growth factor β (TGFβ)-treated fibrotic MRC-5 cells in a specific, dose-dependent manner, saturating at approx. 250 μg mL^-1<i>in vitro</i>, and that our NPs have potent anti-fibrotic activity <i>in vivo</i> in particular, reversing bleomycin-induced fibrosis in mouse lungs, accompanied by marked reduction in profibrotic markers including collagen 1, fibronectin, α-smooth muscle actin, β-catenin and galectin-3. In all, we present an inherently therapeutic inhalable nanocarrier for galectin-3 targeting and anti-fibrotic therapy. We envision this carrier to be doubly effective against fibrotic lung tissue when combined with an encapsulated anti-fibrotic drug, improving overall/total therapeutic efficacy and patient compliance <i>via</i> the reduction of off-target effects and additive therapeutic effects.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11804936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Development of a tannic acid- and silicate ion-functionalized PVA-starch composite hydrogel for <i>in situ</i> skeletal muscle repairing.","authors":"Longkang Li, Huipeng Li, Zhentian Diao, Huan Zhou, Yanjie Bai, Lei Yang","doi":"10.1039/d5tb90025e","DOIUrl":"https://doi.org/10.1039/d5tb90025e","url":null,"abstract":"<p><p>Correction for 'Development of a tannic acid- and silicate ion-functionalized PVA-starch composite hydrogel for <i>in situ</i> skeletal muscle repairing' by Longkang Li <i>et al.</i>, <i>J. Mater. Chem. B</i>, 2024, <b>12</b>, 3917-3926, https://doi.org/10.1039/D3TB03006G.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Core-shell structured microneedles with programmed drug release functions for prolonged hyperuricemia management.","authors":"Rui Wang, Yanfang Sun, Han Wang, Tianqi Liu, Amin Shavandi, Lei Nie, Khaydar E Yunusov, Guohua Jiang","doi":"10.1039/d5tb90023a","DOIUrl":"https://doi.org/10.1039/d5tb90023a","url":null,"abstract":"<p><p>Correction for 'Core-shell structured microneedles with programmed drug release functions for prolonged hyperuricemia management' by Rui Wang <i>et al.</i>, <i>J. Mater. Chem. B</i>, 2024, <b>12</b>, 1064-1076, https://doi.org/10.1039/D3TB02607H.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}