Journal of materials chemistry. B最新文献

{"title":"Polysiloxane-based polyurethane/quaternary ammonium-zwitterionic pressure-sensitive adhesive composite double dressing with antimicrobial and antifouling properties.","authors":"Yichao Hu, Weiguang Jia, Xionghui Wu, Yaling Lin, Anqiang Zhang","doi":"10.1039/d5tb01431j","DOIUrl":"https://doi.org/10.1039/d5tb01431j","url":null,"abstract":"<p><p>The development of wound dressings that combine mechanical strength, flexibility, biocompatibility, and multifunctionality continues to pose significant challenges in biomedical engineering. This study introduces an innovative bilayer polyurethane composite dressing featuring distinct functional layers. The polyurethane protective layer (PUPL), fabricated from polydimethylsiloxane-based polyurethane, functions as a hydrophobic, mechanically robust barrier that simultaneously provides structural integrity, flexibility, and effective protection against fluid penetration and environmental contaminants, thereby minimizing external interference with the wound healing process. The polyurethane functional layer (PUFL) comprises cationically and zwitterionically modified polyurethane pressure-sensitive adhesives, engineered to exhibit optimal differential adhesion properties in both wet and dry conditions along with multifunctional characteristics. This modification strategy harnesses the antimicrobial efficacy of cationic polymers while mitigating potential wound inflammation through zwitterionic ions that reduce cation-induced protein adsorption. Furthermore, incorporating polyethylene glycol into the polyurethane pressure-sensitive adhesive's soft segment enhances the material's hydrophilicity and moisture retention capacity, promoting an optimal moist wound healing environment. The resulting bilayer dressing demonstrates superior mechanical properties, excellent flexibility, and appropriate water vapor transmission rates, enabling secure wound adhesion. Moreover, it exhibits remarkable biocompatibility along with pronounced antimicrobial and antifouling capabilities. The synergistic interaction between the hydrophobic protective layer and the functional adhesive layer offers a promising platform for advancing next-generation wound healing materials.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Au@mSiO₂ nanocomposites with large pores for protein transport.","authors":"Andrea Montero-Oleas, Yoann Roupioz, Philippe Trens, Stéphanie Kodjikian, Silvio J Ludueña, Lía I Pietrasanta, Sara A Bilmes, Xavier Cattoën","doi":"10.1039/d5tb01290b","DOIUrl":"https://doi.org/10.1039/d5tb01290b","url":null,"abstract":"<p><p>Gold-mesoporous silica (Au@mSiO₂) core-shell nanoparticles (NPs) have shown interesting potential for the loading of molecules to be delivered by plasmonic heating. In this study, we describe the unprecedented synthesis of Au@mSiO₂ NPs with large pores (lp-Au@mSiO₂), aiming at encapsulating large biomolecules such as proteins. Starting from recently reported Au@mSiO₂ seeds with a diameter of 45 nm, two strategies are presented to grow a mesoporous shell with large pores. The most interesting NPs (lp-Au@mSiO₂) were obtained with the biphasic stratification approach, yielding NPs with large conical pores (10-20 nm openings), characterized in depth by N₂-sorption and electron microscopies. Atomic force microscopy (AFM) with a sharp tip was used for the first time with these mesoporous materials to probe the accessibility of the pore openings. Biphasic stratification provides NPs with good colloidal and hydrolytic stabilities in aqueous saline medium (PBS) allowing the incubation of these NPs with two model proteins: horse radish peroxidase (HRP) and red fluorescent protein (RFP). lp-Au@mSiO₂ exhibit a significantly larger loading capacity with respect to NPs with similar diameter, either non-porous or with narrower pores, providing evidence that the proteins can indeed be encapsulated within the pores.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipopolysaccharide-imprinted magneto-TiO₂ nanoagents harness dopamine charge transfer to drive visible-light photodynamic therapy for sepsis.","authors":"Jiateng Wu, Jiali Wang, Weige Dong, Yu Wan, Chungu Zhang, Ming-Yu Wu, Shun Feng","doi":"10.1039/d5tb01349f","DOIUrl":"https://doi.org/10.1039/d5tb01349f","url":null,"abstract":"<p><p>Conventional TiO₂-based photodynamic therapy (PDT), which relies on ultraviolet (UV) activation, faces critical limitations including non-specific reactive oxygen species (ROS) generation causing collateral tissue damage, high-power density requirements risking thermal injury, and limited spatiotemporal precision due to broad-spectrum absorption. To address these challenges, we constructed a visible-light-driven nanoplatform through ligand-to-metal charge transfer (LMCT) engineering. The platform, termed LPS-MIP, integrates a polydopamine (PDA) molecular imprinting layer with a Fe₃O₄@SiO₂@TiO₂ core. The PDA layer not only creates pathogen-specific recognition cavities <i>via</i> boronate affinity imprinting for selective <i>P. aeruginosa</i> binding but also establishes an LMCT pathway with TiO₂, shifting its activation spectrum to visible light. This innovation enables UV-free ROS generation under low-intensity white LED light (100 mW cm^-2), eliminating off-target toxicity while achieving complete bacterial eradication within 120 min, with 6.6-fold higher photocurrent density than UV-activated TiO₂. In murine sepsis models, LPS-MIP demonstrated >99% bacterial clearance in the bloodstream, suppressed hyperinflammation (TNF-α/IL-6 reduced to baseline levels), and prevented multiorgan damage, outperforming gentamicin-treated controls. The embedded Fe₃O₄ core enabled rapid magnetic retrieval, reducing hepatic nanoparticle retention by 85%. By replacing UV with biocompatible visible light and confining ROS production to pathogen-binding sites, this design resolves the long-standing trade-off between antimicrobial efficacy and systemic toxicity, offering a clinically adaptable strategy for precision sepsis therapy.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructured gold platforms for attogram-precision cardiolipin quantification.","authors":"Mehrsa Khalilipour, Ahmad Moshaii, Hossein Siampour, Sadaf Yarjoo, Reza H Sajedi, Jahangir Mohammadzadeh","doi":"10.1039/d5tb00798d","DOIUrl":"https://doi.org/10.1039/d5tb00798d","url":null,"abstract":"<p><p>An ultra-sensitive electrochemical immunosensor is presented for the precise quantification of cardiolipin, a key biomarker of mitochondrial dysfunction and cardiovascular pathologies. This innovative platform utilizes two distinct gold nanostructures, nanorods and nanodendrites, within the sensing electrode to achieve exceptional sensitivity and selectivity. These nanostructures were synthesized through a sequential process involving physical vapor deposition of an ultrathin gold film, thermal annealing to nucleate gold seeds, and precision electrochemical deposition, enabling controlled growth on fluorine-doped tin oxide (FTO) substrates. This template-free strategy, optimized through systematic parameter refinement, ensures high reproducibility and electrochemical efficiency. Unlike conventional colloidal synthesis methods that rely on seed-mediated growth and extensive surfactant usage, our template-free electrochemical approach offers a simpler, more reproducible, and surfactant-reduced route for fabricating well-controlled gold nanostructures suitable for biocompatible sensing platforms. Morphological, structural, and surface characterization of the nanostructures was performed using field emission scanning electron microscopy, X-ray diffraction, and contact angle measurements, confirming well-defined growth and controlled surface wettability. Electrochemical analyses, including cyclic voltammetry and electrochemical impedance spectroscopy, further revealed enhanced electron transfer and reduced interfacial resistance, verifying their suitability for sensitive biosensing applications. Functionalization with anti-cardiolipin antibodies ensures high specificity. Electrochemical measurements reveal a dynamic linear range (1 ag mL^-1 to 0.1 pg mL^-1) and detection limits of 0.19 ag mL^-1 (nanorods) and 0.51 ag mL^-1 (nanodendrites). Nanorods demonstrate superior charge transfer, lower impedance, and greater accessibility, positioning this scalable platform as a paradigm shift for lipidomic profiling and advanced diagnostics in precision medicine.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosensitive PLA-cationic porphyrin films: robust antibacterial materials for fighting multidrug-resistant bacteria.","authors":"Carolina V Domingos, Madalena F C Silva, M Ermelinda S Eusébio, Teresa M R Maria, João R A Pires, Gabriela J da Silva, Rafael T Aroso, Mariette M Pereira","doi":"10.1039/d5tb01354b","DOIUrl":"https://doi.org/10.1039/d5tb01354b","url":null,"abstract":"<p><p>The global rise of multidrug-resistant (MDR) bacteria highlights the urgent need for alternative strategies to prevent their spread, particularly in healthcare environments. Here, we report the synthesis and evaluation of a novel mono-cationic <i>meso</i>-imidazolyl porphyrin (3) and its integration into biodegradable poly(lactic acid) (PLA) films, resulting in potentially effective, reusable, and broad-spectrum photodynamic antibacterial surfaces. The porphyrin was prepared using a mixed aldehyde condensation followed by microwave-assisted methylation, reducing reaction time to just 1 minute with near-quantitative yield. PLA films containing porphyrin 3 (+PLA-3) were successfully prepared and fully characterized, showing exceptional photostability and minimal leaching in aqueous environments, even under prolonged light exposure. Notably, cationic +PLA-3 exhibited superior material stability compared to analogous PLA films incorporating tetra-cationic porphyrins, attributed to the improved amphiphilic balance of the mono-cationic photosensitizer. Antibacterial studies confirmed that cationic +PLA-3 films achieved complete photodynamic inactivation (7 log CFU reduction) of both Gram-positive (<i>Staphylococcus aureus</i>) and Gram-negative (<i>Escherichia coli</i>) bacteria under blue LED irradiation. They also demonstrated great effectiveness against clinical multidrug-resistant strains, including MRSA, <i>E. coli</i>, <i>Acinetobacter baumannii</i>, <i>Pseudomonas aeruginosa</i>, and <i>Klebsiella pneumoniae</i>, reaching total inactivation under light doses up to 23.5 J cm^-2. Reusability tests confirmed full retention of antibacterial efficacy after up to 11 irradiation cycles, totalling 258.5 J cm^-2. These results position cationic +PLA-3 as a promising candidate for use in self-disinfecting surfaces with potential to reduce nosocomial infection risks and environmental impact in healthcare settings.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel CRISPR-Cas12a-based diagnostic for rapid and highly sensitive detection of West Nile virus.","authors":"Asli Erol, Dilan Celebi-Birand, Ebru Yilmaz, Ceylan Polat, Ozge Erisoz Kasap, Bulent Alten, Memed Duman","doi":"10.1039/d5tb01268f","DOIUrl":"https://doi.org/10.1039/d5tb01268f","url":null,"abstract":"<p><p>Climate change is increasing the global threat of vector-borne diseases, including West Nile Virus (WNV), a significant human and animal pathogen transmitted primarily by <i>Culex</i> mosquitoes. Current WNV diagnostic methods, while including sensitive techniques like RT-PCR, have limitations in early detection, practicality, and cost-effectiveness. There is an urgent need to develop novel and more efficient strategies to address these challenges and to facilitate the surveillance and management of WNV infections and their spread. This study presents a highly specific and sensitive CRISPR-Cas12a-based detection protocol for WNV detection. Through systematic analysis of key reaction parameters (time: 0-60 min; reporter concentration: 1-80 nM, Cas12a and crRNA concentration: 5.625-90 nM; and template amount: 10^-2-10⁵ pg) and integration with reverse transcriptase recombinase polymerase amplification to enhance sensitivity through an isothermal technique, this assay demonstrates a novel strategy for the rapid detection of WNV, achieving 10 femtomolar sensitivity within one hour. Moreover, the assay retained its efficacy at different temperatures (25 °C and 37 °C) and in biological matrices containing the host (fly or human) genetic material, which supports its applicability in resource-limited settings. Therefore, the method presented here has the potential for broad application in diverse point-of-care settings for rapid diagnosis of WNV.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Au-CNT nanohybrid for highly sensitive catalytic and affinity biosensing applications.","authors":"Aditya Manu Bharti, Terry Ting-Yu Chiou, R K Rakesh Kumar, Muhammad Omar Shaikh, Cheng-Hsin Chuang","doi":"10.1039/d5tb00990a","DOIUrl":"https://doi.org/10.1039/d5tb00990a","url":null,"abstract":"<p><p>Carbon nanotubes (CNTs) have unique intrinsic properties, such as huge surface areas, homogenous pore size distributions, and nanoscale dimensions, making them highly promising for biosensing applications. However, their limited dispersion in biological media and increased resistance hinder efficient charge transfer, necessitating innovative strategies to enhance their performance. This study presents a novel and facile approach for synthesizing multifunctional gold nanoparticle (AuNP)-decorated CNT (Au-CNT) nanohybrids using hetero-functional polyethylene glycol (PEG) as a linker <i>via</i> EDC/NHS chemistry. This approach ensures the covalent and uniform decoration of AuNPs over the surface of CNTs, significantly improving their dispersion, chemical stability, and biocompatibility while preserving their electrochemical activity. Comprehensive morphological, chemical, and electrochemical characterizations confirm improved dispersion and enhanced electron transfer capabilities. To validate its practical application, a proof-of-concept electrochemical sensor is developed for H₂O₂ and glucose detection. Comparative analysis reveals that the Au-CNT nanohybrid exhibits an increase in sensitivity over pristine CNTs, demonstrating its superior catalytic performance. These findings highlight the potential of Au-CNT nanohybrids as versatile platforms for advanced electrochemical catalytic and affinity biosensing applications.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbonised porous silicon as scaffold and sensor for the electrochemical detection and characterisation of bacterial biofilm growth.","authors":"Fearghal O'Connor, Alexandros Lazanas, Beatriz Prieto Simón","doi":"10.1039/d5tb01036e","DOIUrl":"https://doi.org/10.1039/d5tb01036e","url":null,"abstract":"<p><p>The use of carbonised porous silicon (C-pSi) substrates as both scaffolds and electrodes for the electrochemical detection and characterisation of bacterial biofilm growth is reported for the first time. Three types of C-pSi are presented showcasing versatility in their hydrophilicity and surface chemistry. The tunability of these properties is taken as a springboard for the biological, morphological and electrochemical evaluation of the biofilms. The combined characterisation data obtained from environmental scanning electron microscopy, confocal scanning laser microscopy, cyclic voltammetry and electrochemical impedance spectroscopy, confirm C-pSi as a powerful tool for sensing bacterial biofilm growth. The collective assessment of biofilm growth at the C-pSi surface reveals changes related both to morphological and temporal parameters.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone-targeted carbon dots nanogels achieve precision antioxidant therapy for rheumatoid arthritis by activating the endogenous antioxidant system.","authors":"Ruijiao Li, Yingying Zhang, Meng Ma, Haojie Li, Qian He","doi":"10.1039/d5tb01469g","DOIUrl":"https://doi.org/10.1039/d5tb01469g","url":null,"abstract":"<p><p>Carbon dots (CDs) exhibit significant potential as nanozymes for the treatment of rheumatoid arthritis (RA) due to their multi-enzyme mimetic activity. However, their non-specific biodistribution may lead to systemic redox imbalance. Herein, a bone-targeted CD nanogel (n(CD)) was constructed by <i>in situ</i> free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine, forming a three-dimensional porous network that encapsulates multi-enzyme active CDs. The porous polymer shell allows the free diffusion of reactive oxygen species (ROS) and efficiently scavenges them through cascade catalytic reactions. The phosphate groups on the surface of the n(CDs) specifically coordinate with hydroxyapatite in bone tissue, providing precise bone-targeting ability. In collagen-induced arthritis rats, n(CDs) demonstrated prolonged joint retention and effectively suppressed synovial oxidative damage through localized ROS neutralization, while downregulating the expression of pro-inflammatory cytokines and significantly outperforming free CDs. Mechanistic studies revealed that n(CDs) can activate the endogenous antioxidant defense system <i>via</i> upregulation of the heme oxygenase-1 pathway. This study provides a paradigm reference for designing high-specificity nanozyme platforms to treat oxidative stress-related bone and joint diseases through a targeted delivery-<i>in situ</i> catalysis-endogenous activation triple synergy strategy.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144984219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of asymmetric pyridinium-based fluorescent probes with large Stokes shifts for live neural stem/progenitor cells.","authors":"Ye Ri Han, Dongwan Ko, Yeongran Hwang, Myeong A Choi, Do-Geun Kim, Jung Yeol Lee, Beomsue Kim","doi":"10.1039/d5tb00857c","DOIUrl":"https://doi.org/10.1039/d5tb00857c","url":null,"abstract":"<p><p>Fluorescent dyes are widely used in biological systems, yet the number of fluorescent scaffolds with desirable photophysical properties remains limited. Herein, we report the synthesis of novel asymmetric pyridinium salts <i>via</i> facile Rh(III) C-H activation. A structure-activity relationship (SAR) study led to the identification of a lead candidate, KD01, which exhibits a large Stokes shift (Ex/Em = 405/605 nm) and bright fluorescence only upon interaction with live brain cells. Remarkably, KD01 selectively labels undifferentiated human neural stem/progenitor cells (NSPCs) over differentiated neural cells. This selectivity is likely due to the probe's interaction with the unique biochemical or physical properties of the NSPC cytoplasm, such as binding to an abundant biomolecule. This work presents a bioactive fluorescent scaffold with unique optical properties and NSPC selectivity, offering a promising platform for live-cell imaging and targeted neural cell identification.</p>","PeriodicalId":94089,"journal":{"name":"Journal of materials chemistry. B","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144857214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}