Chih-Wen Su, Li-Chin Tsai, Yu-Lin Chung, Yu-Chih Lin, James Chun-I Lee, Adrian Linacre, Hsing-Mei Hsieh
{"title":"Rapid identification of blood using RT-RPA: A pilot study.","authors":"Chih-Wen Su, Li-Chin Tsai, Yu-Lin Chung, Yu-Chih Lin, James Chun-I Lee, Adrian Linacre, Hsing-Mei Hsieh","doi":"10.1111/1556-4029.70464","DOIUrl":"https://doi.org/10.1111/1556-4029.70464","url":null,"abstract":"<p><p>This study details the detection of blood remote from a laboratory using a direct recombinase polymerase amplification reaction integrated with a lateral flow assay. The assay targets the ALAS 2 gene; a marker used in mRNA assays for the identification of blood in body fluid assignments. The process can be used at scenes as no specialized equipment is needed; the assay is efficient between 17°C and at least at 45°C. A result can be obtained in 10 min if the ambient temperature is more than 25°C but this time increases to 40 min if cooler than 25°C. Like any test for a body fluid, the assay needs to be highly sensitive, positive results were obtained with this assay from blood dilute to 0.0005 μL. The test was found to be specific for blood as positive results were only recorded in the presence of blood and no other body fluid. The reagents as a solution were stable for 3 days at 4°C adding to their potential use outside of laboratory conditions. The assay is straightforward to perform yielding results that can be interpreted easily. While not replacing presumptive tests, this can be used to further give confidence in assigning a sample as blood.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yonit Kasherman, Erez Stier, Dan Muller, Ron Chibel, Arnon Grafit, Yaniv Y Avissar
{"title":"Forensic analysis of gasoline residues in urban soil samples: Consequences for vehicle arson investigation.","authors":"Yonit Kasherman, Erez Stier, Dan Muller, Ron Chibel, Arnon Grafit, Yaniv Y Avissar","doi":"10.1111/1556-4029.70470","DOIUrl":"https://doi.org/10.1111/1556-4029.70470","url":null,"abstract":"<p><p>A substantial proportion of forensic fire investigations involve vehicle arson, where severe thermal damage often limits direct evidence recovery. Consequently, soil samples from the vicinity are frequently analyzed for ignitable liquid residues (ILRs). This study evaluates the forensic reliability of gasoline detection in common urban substrates and defines substrate-dependent detection windows. A total of 100 background soil samples from active parking areas were analyzed by gas chromatography-mass spectrometry (GC-MS); no gasoline residues were detected, indicating a low probability of detectable background contamination (<3.2% at 96% confidence). Controlled spill experiments demonstrated marked variability in persistence: up to 36 h in interlocking stone sand, 5 days in bulk sand, 3 weeks in kurkar, and up to 1 year in asphalt. The addition of water prior to sampling substantially extended detection in porous substrates, increasing the detectable time window in kurkar. Gravimetric analysis showed exponential type evaporation behavior for gasoline, with a transition toward linear profiles following water addition. These results demonstrate that gasoline persistence is strongly substrate-dependent and that moisture conditions substantially influence detectability. They improve interpretation of ILR findings in vehicle arson investigations and support further consideration of targeted pre-sampling water application as a potential approach to enhance ILR recovery from porous substrates.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lillian S Antonelli, David E Raymond, Ashley E Kendell, Eric J Bartelink, Mariyam I Isa, Mathias Brieu
{"title":"Testing the utility of porcine and synthetic femora as nonhuman analogs in skeletal trauma research.","authors":"Lillian S Antonelli, David E Raymond, Ashley E Kendell, Eric J Bartelink, Mariyam I Isa, Mathias Brieu","doi":"10.1111/1556-4029.70467","DOIUrl":"https://doi.org/10.1111/1556-4029.70467","url":null,"abstract":"<p><p>Porcine and synthetic femora were subjected to concentrated 4-point bending, modified 4-point bending, and 3-point bending to generate diaphyseal fractures. Biomechanical data and fracture patterns were systematically evaluated and compared across different femur types and loading conditions. Biomechanical and fracture data from Isa et al. [11] were employed as a comparative human sample. Loading condition did not significantly affect mechanical behavior or fracture type in porcine femora and had limited effects on synthetic femora. Further, the results of the concentrated 4-point bending tests on porcine and synthetic femora were compared to published data on human femora subjected to the same loading condition. Although some similarities in fracture type outcomes were observed between porcine and human femora, these similarities do not outweigh the substantial differences between species in detailed fracture patterning. These results suggest that neither porcine nor synthetic femora adequately replicate human fracture patterns and thus are inappropriate analogs for understanding long bone fracture patterns relevant to forensic anthropology casework. Although nonhuman analogs are commonly used in experimental trauma research, additional studies are needed to investigate the appropriateness of these analogs for modeling specific types of skeletal trauma.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Forensic challenges in a case of suspected filicide, matricide, and post-mortem dismemberment.","authors":"Isabella Caristo, Rosario Barranco, Giulio Fraternali Orcioni, Domenico Coviello, Francesco Ventura","doi":"10.1111/1556-4029.70465","DOIUrl":"https://doi.org/10.1111/1556-4029.70465","url":null,"abstract":"<p><p>Familicide represents one of the most complex forms of domestic violence in forensic practice. Among intrafamilial homicides, filicide and matricide pose major medico-legal and criminological challenges, particularly when autopsy findings are nonspecific or severely altered by post-mortem changes. We report an unusual forensic case involving the deaths of a 3-year-old child and his grandmother, both initially characterized by uncertain manners of death. The child was found dead by his mother while sleeping in a shared bed with multiple pillows. Autopsy examination revealed facial injuries and histological findings consistent with mechanical asphyxia, including diffuse alveolar hemorrhage, pulmonary emphysema, and rupture of the alveolar septa. Toxicological analyses were negative. Genetic investigations identified variants of uncertain significance associated with cardiomyopathies; however, parental testing demonstrated inherited variants, making a genetic arrhythmic cause unlikely. Circumstantial inconsistencies and internet searches performed by the mother regarding suffocation methods further supported homicidal smothering. Epidemiological evidence indicating that accidental sleep-related suffocation occurs predominantly during infancy made the child's age atypical for an accidental mechanism. Five months later, the dismembered and partially skeletonized body of the child's grandmother was discovered inside the family home. Autopsy and histological findings suggested death due to neck compression, although advanced putrefaction and extensive post-mortem dismemberment prevented definitive differentiation between suicidal hanging and homicidal strangulation. This case highlights the critical importance of integrating autopsy, histological, toxicological, genetic, and investigative findings in complex intrafamilial deaths, particularly when traumatic signs are limited or post-mortem destruction compromises forensic interpretation.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kelly A Waters, Makayla Guenther, Katherine H Yoskowitz, Audrey Malone, Anthony Grigsby, Bethany G Mikles, Jennifer L Pechal, M Eric Benbow
{"title":"Effects of bleach on carcass postmortem microbiomes and necrophagous beetle carcass attendance and their internal microbiomes.","authors":"Kelly A Waters, Makayla Guenther, Katherine H Yoskowitz, Audrey Malone, Anthony Grigsby, Bethany G Mikles, Jennifer L Pechal, M Eric Benbow","doi":"10.1111/1556-4029.70460","DOIUrl":"https://doi.org/10.1111/1556-4029.70460","url":null,"abstract":"<p><p>Forensic entomology, and potentially microbiology in the future, can be used during death investigations to corroborate an estimated time since death. Instances where products are introduced to a cadaver, either in attempt to conceal or by accident, can affect decomposition and succession of associated decomposer taxa (i.e., insects and bacteria). In this study, we tested the effect of two bleach (sodium hypochlorite) submersions on the outdoor decomposition and succession of carcass, soil, and beetle postmortem microbiomes using replicate (n = 5 per treatment) stillborn swine carcasses. Microbiomes from beetles, the carcasses, and the soil beneath them were sequenced from samples collected every two days. Beetle communities collected from pitfall traps were not significantly different between bleached and control carcasses through decomposition; however, there were fewer beetles collected from bleached carcasses until the last experimental day. Results from 16S rRNA amplicon sequencing revealed that microbiomes of carcasses and the soil beneath them did not significantly change when carcasses were bleached, and beetle internal microbiomes were not affected by the addition of bleach. Random forest modeling using soil microbiomes from underneath carcasses from both treatments was used to predict time since placement in the field with a 74.4% successful prediction rate, and modeling using beetle internal microbiomes predicted beetle family with 74.0% success. The introduction of bleach to the carcasses did not affect microbial or beetle communities, total decomposition time, or decrease accuracy of time of placement models.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jonathan Heckeroth, Axel Kerkhoff, Paul Schmidt, Raymond Marquis, Erich Kupferschmid, Céline Weyermann
{"title":"Exploring feature variability in digitally captured signatures-Part I: Variability in genuine signatures and the effects of collection sessions and writing position.","authors":"Jonathan Heckeroth, Axel Kerkhoff, Paul Schmidt, Raymond Marquis, Erich Kupferschmid, Céline Weyermann","doi":"10.1111/1556-4029.70457","DOIUrl":"https://doi.org/10.1111/1556-4029.70457","url":null,"abstract":"<p><p>Digitally captured signatures (DCSs) pose new challenges for forensic handwriting examination compared with paper-based signatures. While DCSs provide spatial coordinates, time and force values for forensic examination, the variability of the features derived from these data remains insufficiently understood. This study therefore examined the within- and between-writer variability in 1560 genuine DCSs collected from 65 participants across eight sessions and three writing positions. These signatures were treated as questioned specimens and compared with 1820 reference DCSs collected later in one writing position. As expected, the results revealed substantial between-writer differences in absolute feature values, in variability, and in correlations despite general patterns across writers. Depending on the feature, between 55% (line length) and 96% (number of pen lifts) of the questioned signatures fell within their writer-specific reference ranges. Most values that were outside the range clustered around the reference distributions. This highlighted the limitations of interpretation based on a \"within-outside the reference range\" threshold. Across the eight sessions, only four out of the fifteen examined features (DTW scores for XY coordinates, time in air, total duration, and width) showed systematic changes, with questioned signatures becoming increasingly similar to the subsequently collected reference samples. Writing position also influenced several features significantly; for example, median force was lower when the pad was held in hand. The findings of this study underscore the importance of accounting for writer-specific feature variability and non-standard writing conditions when examining DCSs in forensic casework.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148908378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of circumstances in police-related fatalities comparing medical examiner records with a public database.","authors":"Richard C Harruff, J Matthew Lacy, Nicole A Yarid","doi":"10.1111/1556-4029.70369","DOIUrl":"10.1111/1556-4029.70369","url":null,"abstract":"<p><p>Assessing circumstances surrounding police-related deaths is hampered by the absence of an official national database. The National Vital Statistics System, which compiles death certificates filed by medical examiners and coroners, misses approximately one-half of deaths due to police use of force. The void has been filled by the emergence of several unofficial databases sourced from public records, which have been used, for example, to estimate the number of fatal police shootings of unarmed individuals. However, these public databases have not been validated against official records. Furthermore, there have been no attempts to explore the complexity of circumstances leading to police-related fatalities. The present study was conducted to compare information in one public database, Fatal Encounters (FE), with matched records from the King County Medical Examiner's Office (ME) and to measure the role of overlapping circumstances leading to fatal outcomes. For this purpose, the two data sources were linked by names. Circumstantial elements were extracted, categorized, and analyzed according to Manner of Death, Pre-Custody criteria, and Race assignments. Overlapping circumstances were resolved by the calculation of conditional probabilities. The results found 172 matched records in which agreements between the two sources ranged from 70% to 100%. Disagreements were present in whether the decedent was unarmed and not fleeing. The distribution of deaths according to race was disproportionate relative to the county population. The study demonstrated the importance of data in measuring police-related fatalities and the need for caution in interpreting data that does not fully encompass the range of complex circumstances.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":"2217-2228"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147978162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
William H Shirley, Alexander J Jutila, Kelly A Waters, Anthony Grigsby, M Eric Benbow, Ruth Waddell Smith
{"title":"Considerations for multivariate approaches to classify blow fly species based on cuticular hydrocarbon profiles.","authors":"William H Shirley, Alexander J Jutila, Kelly A Waters, Anthony Grigsby, M Eric Benbow, Ruth Waddell Smith","doi":"10.1111/1556-4029.70374","DOIUrl":"10.1111/1556-4029.70374","url":null,"abstract":"<p><p>Blow flies are early colonizers of human remains, allowing their developmental rates to be used to estimate postmortem interval (PMI). Accurate species identification is essential for PMI estimation, yet traditional methods can be subjective. Recently, the use of cuticular hydrocarbons (CHCs) for more objective species identification has been demonstrated. These studies generally use multivariate statistical approaches to evaluate differences in CHC profiles among different species. Principal component analysis (PCA) is among the more common procedures used to evaluate these differences. However, PCA often requires data pretreatment steps to address inherent biological differences among specimens, which can result in the loss of meaningful biological trends. Further, few studies have developed the predictive classification models necessary for downstream PMI estimation. In this work, CHCs were extracted from adult females of seven blow fly species and analyzed by gas chromatography-mass spectrometry (GC-MS). Profiles were initially evaluated with PCA and non-metric multidimensional scaling (NMDS). From PCA, six of the seven species were distinguished, but distinction was influenced heavily by abundance differences due to inherent variability among specimens. From NMDS, tighter species clusters were observed without the need for data pretreatment. Classification models were then developed using partial least squares-discriminant analysis (PLS-DA) and random forest modeling. Both models demonstrated high classification success for cross validation (98.67% and 98.67%) and external validation (66.67% and 61.11%), although the random forest classifier had the advantage of requiring no data pretreatment. This work demonstrates successful species classification and highlights considerations for the statistical evaluation of CHC profiles.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":"2240-2253"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148140557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harshwardhan Khushalrao Khartade, Priyanka Meshram, Shikha Shrivastava, Mohammed Akbar N J, Vikas Meshram
{"title":"Illegal electric fencing acting as a death trap in a suspected homicide.","authors":"Harshwardhan Khushalrao Khartade, Priyanka Meshram, Shikha Shrivastava, Mohammed Akbar N J, Vikas Meshram","doi":"10.1111/1556-4029.70380","DOIUrl":"10.1111/1556-4029.70380","url":null,"abstract":"<p><p>Electrocution fatalities most commonly occur in domestic or occupational settings. When such deaths are found in remote outdoor locations, they often raise suspicion of homicide or deliberate body disposal. Deaths due to illegal electric fencing are rare and usually accidental, occurring in rural or forest-fringe agricultural areas. Fatalities resulting specifically from direct tapping of high-voltage transmission lines into fences are exceedingly uncommon and sparsely documented in the forensic literature. We report a case of an unidentified decomposed male body recovered from a secluded mountainous area, initially suspected to be a homicidal dumping. An autopsy and clothing examination revealed characteristic high-voltage electrical injuries. The absence of an electrical source at the recovery site suggested postmortem relocation of the body. Subsequent investigation identified an illegally electrified farm fence connected directly to an overhead high-voltage line. The victim had accidentally come into contact with the fence at night and died instantly. To evade legal consequences, the farmer and his associates disposed of the body at a distant location to simulate a suicide. This case highlights the importance of integrating autopsy findings, clothing examination, and scene reconstruction to determine the cause and manner of death in concealed electrocution due to illegal electric fencing.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":"2455-2460"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148166145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Forensic timeline investigation of Apple Health app on iOS.","authors":"Annisa Dwi Puspitarini, Hudan Studiawan","doi":"10.1111/1556-4029.70385","DOIUrl":"10.1111/1556-4029.70385","url":null,"abstract":"<p><p>Apple Health data provide critical forensic insights into a user's physical activities, exercise routines, sleep patterns, and biometrics. However, the complexity of its database schema and frequent iOS updates pose significant challenges for automated forensic analysis. This research develops and integrates custom plugins into the log2timeline Plaso framework to automate the extraction of Apple Health records. The developed parsers were tested across multiple iterations, specifically addressing the database reorganizations found in iOS 13.3.1, iOS 13.4.1, iOS 15.3.1, iOS 16.1.2, and iOS 17.3. Results demonstrate the developed plugins outperform iLEAPP (iOS Logs, Events, and Plist Parser) by successfully extracting Apple Health features that previously unrecoverable. The plugins successfully integrate disparate schema variations across multiple iOS versions into a unified timeline, reducing manual analysis effort. This study concludes that adaptive plugins is essential for maintaining forensic tool efficacy amidst rapid mobile iOS evolution, providing a robust solution for timeline-based health data investigations.</p>","PeriodicalId":94080,"journal":{"name":"Journal of forensic sciences","volume":" ","pages":"2373-2385"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148378956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}