Journal of biomedical materials research. Part A最新文献

Decellularized Extracellular Matrix and Polyurethane Vascular Grafts Have Positive Effects on the Inflammatory and Pro-Thrombotic State of Aged Endothelial Cells.

Journal of biomedical materials research. Part A Pub Date : 2024-11-29 DOI: 10.1002/jbm.a.37830

Sophie J Specht, Sabrina Rohringer, Pia Hager, Christian Grasl, Anna-Maria Schmitt, Virginia J C Pach, Katharina Ehrmann, Stefan Baudis, Robert Liska, Herbert Kiss, Karl H Schneider, Bruno K Podesser, Helga Bergmeister

{"title":"Decellularized Extracellular Matrix and Polyurethane Vascular Grafts Have Positive Effects on the Inflammatory and Pro-Thrombotic State of Aged Endothelial Cells.","authors":"Sophie J Specht, Sabrina Rohringer, Pia Hager, Christian Grasl, Anna-Maria Schmitt, Virginia J C Pach, Katharina Ehrmann, Stefan Baudis, Robert Liska, Herbert Kiss, Karl H Schneider, Bruno K Podesser, Helga Bergmeister","doi":"10.1002/jbm.a.37830","DOIUrl":"https://doi.org/10.1002/jbm.a.37830","url":null,"abstract":"In vitro assessment of small-diameter synthetic vascular grafts usually uses standard cell culture conditions with early-passage cells. However, these conduits are mainly implanted in elderly patients and are subject to complex cellular interactions influenced by age and inflammation. Understanding these factors is central to the development of vascular grafts tailored to the specific needs of patients. In this study, the effects of aged endothelial cells subjected to pro- and anti-inflammatory agents and cultivated on a newly developed biodegradable electrospun thermoplastic polyurethane/poly(urethane-urea) blend (TPU/TPUU), on clinically available expanded polytetrafluorethylene (ePTFE), and on decellularized extracellular matrix (dECM) grafts were investigated. Young and aged endothelial cells were exposed to pro- and anti-inflammatory agents and characterized by morphology, migration capacity, and gene expression. In addition, the cells were seeded onto the various graft materials and examined microscopically alongside gene expression analyses. When exposed to pro-inflammatory cytokines, young and aged cells demonstrated signs of endothelial activation. Cells seeded on ePTFE showed reduced attachment and increased expression of pro-inflammatory genes compared with the other materials. dECM and TPU/TPUU substrates provided better support for endothelialization with aged cells under inflammatory conditions compared with ePTFE. Moreover, TPU/TPUU showed positive effects on reducing pro-thrombotic and pro-inflammatory gene expression in endothelial cells. Our results thus emphasize the importance of developing new synthetic graft materials as an alternative for clinically used ePTFE.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PLLA Porous Scaffold as a 3D Breast Cancer Model to Investigate Drug Resistance. 将聚乳酸（PLLA）多孔支架作为研究抗药性的三维乳腺癌模型

Journal of biomedical materials research. Part A Pub Date : 2024-11-16 DOI: 10.1002/jbm.a.37836

Camilla Carbone, Salvatrice Rigogliuso, Valerio Maria Bartolo Brucato, Alessandra Cusimano, Manuela Labbozzetta, Vincenzo La Carrubba, Paola Poma, Monica Notarbartolo, Francesco Carfì Pavia

{"title":"PLLA Porous Scaffold as a 3D Breast Cancer Model to Investigate Drug Resistance.","authors":"Camilla Carbone, Salvatrice Rigogliuso, Valerio Maria Bartolo Brucato, Alessandra Cusimano, Manuela Labbozzetta, Vincenzo La Carrubba, Paola Poma, Monica Notarbartolo, Francesco Carfì Pavia","doi":"10.1002/jbm.a.37836","DOIUrl":"https://doi.org/10.1002/jbm.a.37836","url":null,"abstract":"Multidrug resistance remains one of the major challenges in breast cancer research, often leading to treatment failure. To better understand this mechanism, sophisticated three-dimensional (3D) tumor models are necessary, as they offer several advantages over traditional bidimensional (2D) cultures. In this study, poly-l-lactic-acid porous scaffolds were produced using a thermally induced phase separation technique and employed as 3D models for breast cancer cell lines: MDA-MB-231, MCF-7, and its multidrug-resistant variant, MCF-7R. The MTS assay was used to compare growth inhibition following doxorubicin treatment in 2D and 3D. Remarkably, the IC50 values increased in 3D cultures compared to 2D: MDA-MB-231 (445 vs. 54.5 ng/mL), MCF-7 (7.45 vs. 0.75 μg/mL), and MCF-7R (165 vs. 39 μg/mL). MCF-7R, which usually shows greater resistance in 2D, demonstrated even higher resistance in 3D. In fact, IC50 was not reached within 3 days as with the other models, but only after 6 days. Cellular morphology also played a crucial role. When treated with concentrations higher than the IC50, MDA-MB-231 cells lost their physiological 3D clustered structure, while MCF-7 and its resistant variant exhibited disrupted layers. All cell lines in 3D showed higher chemoresistance, suggesting a more biomimetic spatial architecture. Our work bridges the gap between monolayer and animal models, highlighting the potential of polymeric 3D scaffolds in breast cancer research.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topography-Mediated Induction of Epithelial Mesenchymal Transition via Alumina Textiles for Potential Wound Healing Applications. 通过氧化铝纺织品诱导上皮间充质转化的拓扑结构，实现潜在的伤口愈合应用。

Journal of biomedical materials research. Part A Pub Date : 2024-11-11 DOI: 10.1002/jbm.a.37826

Deepanjalee Dutta, Titinun Nuntapramote, Maren Rehders, Klaudia Brix, Dorothea Brüggemann

{"title":"Topography-Mediated Induction of Epithelial Mesenchymal Transition via Alumina Textiles for Potential Wound Healing Applications.","authors":"Deepanjalee Dutta, Titinun Nuntapramote, Maren Rehders, Klaudia Brix, Dorothea Brüggemann","doi":"10.1002/jbm.a.37826","DOIUrl":"https://doi.org/10.1002/jbm.a.37826","url":null,"abstract":"Substrate topography is vital in determining cell growth and fate of cellular behavior. Although current in vitro studies of the underlying cellular signaling pathways mostly rely on their induction by specific growth factors or chemicals, the influence of substrate topography on specific changes in cells has been explored less often. This study explores the impact of substrate topography, specifically the tricot knit microfibrous structure of alumina textiles, on cell behavior, focusing on fibroblasts and keratinocytes for potential wound healing applications. The textiles, studied for the first time as in vitro substrates, demonstrated support for keratinocyte adhesion, leading to alterations in cell morphology and the expression of E-cadherin and fibronectin. These topography-induced changes resembled the epithelial-to-mesenchymal transition (EMT), crucial for wound healing, and were specific to keratinocytes and absent in identically treated fibroblasts. Biochemically induced EMT in keratinocytes cultured on flat alumina substrates mirrored the changes seen with alumina textiles alone, suggesting the tricot knit microfibrous topography could serve as an in vitro model system to induce EMT-like mechanisms. These results enhance our understanding of how substrate topography influences EMT-related processes in wound healing, paving the way for further evaluation of microfibrous alumina textiles as innovative wound dressings.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatibility and Antibacterial Potential of Tetrahedral Amorphous Carbon (ta-C) Coatings on CoCrMo Alloy for Articulating Implant Surfaces. 用于关节植入物表面的 CoCrMo 合金四面体无定形碳 (ta-C) 涂层的生物相容性和抗菌潜力。

Journal of biomedical materials research. Part A Pub Date : 2024-11-07 DOI: 10.1002/jbm.a.37815

Patrick Fabisch, Vadym Voropai, Maren Nieher, Adrian Buchholz, Steffen Weissmantel, Christoph H Lohmann, Jessica Bertrand, Joachim Döring

{"title":"Biocompatibility and Antibacterial Potential of Tetrahedral Amorphous Carbon (ta-C) Coatings on CoCrMo Alloy for Articulating Implant Surfaces.","authors":"Patrick Fabisch, Vadym Voropai, Maren Nieher, Adrian Buchholz, Steffen Weissmantel, Christoph H Lohmann, Jessica Bertrand, Joachim Döring","doi":"10.1002/jbm.a.37815","DOIUrl":"https://doi.org/10.1002/jbm.a.37815","url":null,"abstract":"Premature implant failure, a critical concern in biomedical applications, is often attributed to poor biocompatibility and vulnerability to bacterial colonization. These issues are addressed by creating an endoprosthetic material with natural biocompatibility and antibacterial properties. In this in vitro study, the relaxed and unrelaxed tetrahedral amorphous carbon (ta-C) coatings were examined, both fabricated by the improved patented Pulsed Laser Deposition (PLD) technology. The chemical composition, surface roughness, hardness, topography, and wettability were analyzed. The ta-C surfaces were incubated by MM6 cells, E. coli and S. capitis bacteria for 24 h. PCR assessed the inflammatory response in MM6 cells, while fluorescence microscopy quantified adhering bacteria, and scanning electron microscopy examined local adhesion behavior. The results demonstrate comparable carbon phase composition, wettability properties, and hardness for both relaxed and unrelaxed ta-C. However, relaxed ta-C coating exhibited significantly fewer defects in terms of both quantity and quality, along with an antibacterial effect against E. coli. This suggests that the relaxed ta-C coating could contribute to the development of an endoprosthesis, preventing adverse biological reactions and implant-related infections, thus improving the longevity of the prosthesis.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Marine-Sourced Hydroxyapatite, Chitosan, Collagen, and Gelatin for Eco-Friendly Bone and Cartilage Regeneration. 创新性的海洋来源羟基磷灰石、壳聚糖、胶原蛋白和明胶用于生态友好型骨和软骨再生。

Journal of biomedical materials research. Part A Pub Date : 2024-11-07 DOI: 10.1002/jbm.a.37833

Hoda Elkhenany, Mariam Waleed Soliman, Dina Atta, Nagwa El-Badri

{"title":"Innovative Marine-Sourced Hydroxyapatite, Chitosan, Collagen, and Gelatin for Eco-Friendly Bone and Cartilage Regeneration.","authors":"Hoda Elkhenany, Mariam Waleed Soliman, Dina Atta, Nagwa El-Badri","doi":"10.1002/jbm.a.37833","DOIUrl":"https://doi.org/10.1002/jbm.a.37833","url":null,"abstract":"In recent years, the exploration of sustainable alternatives in the field of bone tissue engineering has led researchers to focus on marine waste byproducts as a valuable resource. These marine resources, often overlooked remnants of various industries, exhibit a rich composition of hydroxyapatite, collagen, calcium carbonate, and other minerals essential to the complex framework of bone structure. Marine waste by-products can emit gases such as methane and carbon dioxide, highlighting the urgency to repurpose these materials for innovative tissue regeneration solutions, offering a sustainable approach to address environmental challenges while advancing medical science. Using these discarded materials offers a promising pathway for sustainable development in regenerative medicine. This review investigates the distinctive properties of marine waste byproducts, emphasizing their capacity to be recycled effectively to contribute to the rebuilding of bone and cartilage tissue during regeneration processes. We also highlight the compatibility of these resources with biological materials such as platelet-rich plasma (PRP), stem cells, exosomes, and natural bioproducts, as well as nanoparticles (NPs) and polymers. By using the natural potential of these resources, we simultaneously address environmental challenges and promote innovative solutions in skeletal tissue engineering, initiating a new era of environmentally green biomedical research.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elastomeric and Conductive Nerve Conduits From Poly(Glycerol-Sebacate)/Carbon Nanofibers (PGS/CNFs). 聚（甘油-蔗糖酸酯）/碳纳米纤维（PGS/CNF）的弹性和导电神经导管。

Journal of biomedical materials research. Part A Pub Date : 2024-11-07 DOI: 10.1002/jbm.a.37820

Bengisu Topuz, Dincer Gokcen, Halil Murat Aydin

{"title":"Elastomeric and Conductive Nerve Conduits From Poly(Glycerol-Sebacate)/Carbon Nanofibers (PGS/CNFs).","authors":"Bengisu Topuz, Dincer Gokcen, Halil Murat Aydin","doi":"10.1002/jbm.a.37820","DOIUrl":"https://doi.org/10.1002/jbm.a.37820","url":null,"abstract":"Many patients suffer from peripheral nerve injury, which can impair their quality of life. Restoring nerve tissue is difficult due to the low ability of nerves to regenerate. Nerve conduits are designed to help peripheral nerve regeneration by providing a scaffold that can match the tissue characteristics, facilitate cellular activities, and be easily implanted. In order to provide a nerve conduit having scaffolding properties, conductance cytocompatibility, we have investigated the potential of channeled structures made of poly (glycerol-sebacate) (PGS) elastomer containing carbon nanofibers (CNFs) in the regeneration of nerve tissue. The first step was to synthesize PGS elastomer and tune its properties to match the nerve tissue. Then, a carbon dioxide laser was used to create micro channels on the elastomer surface for guiding nerve cells. The PGS elastomer was blended with carbon nanofiber (CNF), which was functionalized to bond with the elastomer, to form a conductive structure. The constructs were investigated in terms of cell behavior using PC12 and S42 cell lines. A statistically significant increase in cell proliferation was observed in both cell lines. It was found that the cells began to grow along the canal in places. In terms of elasticity, conductance and cell response, these constructs may be a potential candidate for nerve tissue engineering.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thrombin Immobilized Hemocompatible Radiopaque Polyurethane Microspheres for Topical Blood Coagulation. 用于局部血液凝固的凝血酶固定化血液兼容不透射线聚氨酯微球。

Journal of biomedical materials research. Part A Pub Date : 2024-11-07 DOI: 10.1002/jbm.a.37828

Sonali S Naik, Arun Torris, Gorakh Hiraman Ghuge, V K Karthika, Roy Joseph, Kiran Sukumaran Nair

{"title":"Thrombin Immobilized Hemocompatible Radiopaque Polyurethane Microspheres for Topical Blood Coagulation.","authors":"Sonali S Naik, Arun Torris, Gorakh Hiraman Ghuge, V K Karthika, Roy Joseph, Kiran Sukumaran Nair","doi":"10.1002/jbm.a.37828","DOIUrl":"https://doi.org/10.1002/jbm.a.37828","url":null,"abstract":"Over the past decade, there has been growing interest in developing microspheres for embolization procedures. However, the lack of noninvasive monitoring of the embolic agents and the occurrence of reflux phenomenon leading to unintentional occlusions has raised concerns regarding their compatibility/suitability for embolization therapy. Here we report the development of specialty microspheres having intrinsic radiopacity and surface functionality to tackle the existing complications that pave the way for more advanced solutions. To achieve the above goal, an iodinated monomer, termed \"IBHV,\" capable of imparting radiopacity and functionality, was synthesized and used as a chain extender to make radiopaque polyurethane. Microspheres with a smooth surface and an average diameter of 474 ± 73 μm were fabricated from this polyurethane. The microspheres obtained were noncytotoxic, had a permissible hemolysis rate, and showed better traceability on x-ray imaging. Subsequent immobilization of thrombin onto microspheres improved their hemostatic effect. This study demonstrated that immobilization of thrombin would lead to microspheres with unique traits of radiopacity and hemostatic properties, which will undoubtedly enhance embolization efficiency.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shape Memory Polyurethane Foams With Tunable Mechanical Properties and Radiation Tolerance for Breast Repair and Reconstruction. 用于乳房修复和重建的具有可调机械性能和辐射耐受性的形状记忆聚氨酯泡沫。

Journal of biomedical materials research. Part A Pub Date : 2024-11-06 DOI: 10.1002/jbm.a.37821

Kawun Chung, Xiaojuan Feng, Yuanzhang Jiang, Ka Li, Jianming Chen, Yanting Han, Lin Tan, Zhenggui Du

{"title":"Shape Memory Polyurethane Foams With Tunable Mechanical Properties and Radiation Tolerance for Breast Repair and Reconstruction.","authors":"Kawun Chung, Xiaojuan Feng, Yuanzhang Jiang, Ka Li, Jianming Chen, Yanting Han, Lin Tan, Zhenggui Du","doi":"10.1002/jbm.a.37821","DOIUrl":"https://doi.org/10.1002/jbm.a.37821","url":null,"abstract":"This study developed a shape memory polyurethane foam (SM-PUF) with tunable mechanical properties and exceptional radiation tolerance for potentially implanting tissue defects after mastectomy. The PUFs were synthesized via an in situ foaming strategy using water as a foaming agent, incorporating 4,4'-diphenylmethane diisocyanate (MDI) as the rigid segment and both polyoxytetramethylene glycol and polycaprolactone as the soft segment. The resultant PUFs possess an open-cell structure with a pore size of 30 ~ 800 μm, which achieves a compressive stress of 0.04 MPa under 70% compression strain and a tensile elongation of 667.9%. PUFs exhibit body temperature (37°C)-responsive softening and shape memory abilities, with recovery and fixation ratios reaching 88% and 98%, respectively. It was verified that PUFs can resist 40 Gy radiotherapy without changing their mechanical properties and biocompatibility. This study introduces an innovative approach to produce customizable foam for the reconstruction of implant prostheses for the breast.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Bioprinting a Novel Skin Co-Culture Model Using Human Keratinocytes and Fibroblasts. 利用人体角质形成细胞和成纤维细胞的三维生物打印新型皮肤共培养模型

Journal of biomedical materials research. Part A Pub Date : 2024-11-02 DOI: 10.1002/jbm.a.37831

Thiago A M Andrade, Victor Allisson da Silva, Kali Scheck, Tania Garay, Ruchi Sharma, Stephanie M Willerth

{"title":"3D Bioprinting a Novel Skin Co-Culture Model Using Human Keratinocytes and Fibroblasts.","authors":"Thiago A M Andrade, Victor Allisson da Silva, Kali Scheck, Tania Garay, Ruchi Sharma, Stephanie M Willerth","doi":"10.1002/jbm.a.37831","DOIUrl":"https://doi.org/10.1002/jbm.a.37831","url":null,"abstract":"3D bioprinting can generate the organized structures found in human skin for a variety of biological, medical, and pharmaceutical applications. Challenges in bioprinting skin include printing different types of cells in the same construct while maintaining their viability, which depends on the type of bioprinter and bioinks used. This study evaluated a novel 3D bioprinted skin model containing human keratinocytes (HEKa) and human dermal fibroblasts (HDF) in co-culture (CC) using a high-viscosity fibrin-based bioink produced using the BioX extrusion-based bioprinter. The constructs containing HEKa or HDF cells alone (control groups) and in CC were evaluated at 1, 10, and 20 days after bioprinting for viability, immunocytochemistry for specific markers (K5 and K10 for keratinocytes; vimentin and fibroblast specific protein [FSP] for fibroblasts). The storage, loss modulus, and viscosity properties of the constructs were also assessed to compare the effects of keratinocytes and fibroblasts individually and combined, providing important insights when bioprinting skin. Our findings revealed significantly higher cell viability in the CC group compared to individual keratinocyte and fibroblast groups, suggesting the combined cell presence enhanced survival rates. Additionally, proliferation rates of both cell types remained consistent over time, indicating non-competitive growth within the construct. Interestingly, keratinocytes exhibited a greater impact on the viscoelastic properties of the construct compared to fibroblasts, likely due to their larger size and arrangement. These insights contribute to optimizing bioprinting strategies for skin tissue engineering and emphasize the important role of different cell types in 3D skin models.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142565366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Polycaprolactone Nanofibers for the Proof-of-Concept Construction of the Alveolar-Capillary Interface. 使用聚己内酯纳米纤维构建肺泡-毛细血管界面的概念验证。

Journal of biomedical materials research. Part A Pub Date : 2024-10-30 DOI: 10.1002/jbm.a.37824

Michaela Capandova, Veronika Sedlakova, Zbynek Vorac, Hana Kotasova, Jana Dumkova, Lukas Moran, Josef Jaros, Matej Antol, Dasa Bohaciakova, Ales Hampl

{"title":"Using Polycaprolactone Nanofibers for the Proof-of-Concept Construction of the Alveolar-Capillary Interface.","authors":"Michaela Capandova, Veronika Sedlakova, Zbynek Vorac, Hana Kotasova, Jana Dumkova, Lukas Moran, Josef Jaros, Matej Antol, Dasa Bohaciakova, Ales Hampl","doi":"10.1002/jbm.a.37824","DOIUrl":"https://doi.org/10.1002/jbm.a.37824","url":null,"abstract":"The alveolar-capillary interface is the key functional element of gas exchange in the human lung, and disruptions to this interface can lead to significant medical complications. However, it is currently challenging to adequately model this interface in vitro, as it requires not only the co-culture of human alveolar epithelial and endothelial cells but mainly the preparation of a biocompatible scaffold that mimics the basement membrane. This scaffold should support cell seeding from both sides, and maintain optimal cell adhesion, growth, and differentiation conditions. Our study investigates the use of polycaprolactone (PCL) nanofibers as a versatile substrate for such cell cultures, aiming to model the alveolar-capillary interface more accurately. We optimized nanofiber production parameters, utilized polyamide mesh UHELON as a mechanical support for scaffold handling, and created 3D-printed inserts for specialized co-cultures. Our findings confirm that PCL nanofibrous scaffolds are manageable and support the co-culture of diverse cell types, effectively enabling cell attachment, proliferation, and differentiation. Our research establishes a proof-of-concept model for the alveolar-capillary interface, offering significant potential for enhancing cell-based testing and advancing tissue-engineering applications that require specific nanofibrous matrices.","PeriodicalId":94066,"journal":{"name":"Journal of biomedical materials research. Part A","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0