International journal of radiation biology最新文献

{"title":"Identification and validation of soft tissue sarcoma-specific transcriptomic model for predicting radioresistance.","authors":"Jae Yun Moon, Jae Berm Park, Kyo Won Lee, Daechan Park, Gyu Sang Yoo, Changhoon Choi, Sohee Park, Jeong Il Yu, Do Hoon Lim, Jung Eun Kim, Sung Joo Kim, Woo-Yoon Park, Won Dong Kim","doi":"10.1080/09553002.2024.2447509","DOIUrl":"10.1080/09553002.2024.2447509","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to identify the transcriptomic signatures of soft tissue sarcoma (STS) related to radioresistance and establish a model to predict radioresistance.</p><p><strong>Materials and methods: </strong>Nine STS cell lines were cultured. Adenosine triphosphate-based viability was determined 5 days after irradiation with 8 Gy of X-rays in a single fraction. Radiosensitive and radioresistant groups were stratified according to the survival rates. Whole transcriptomic sequencing analysis was performed and differentially expressed genes (DEGs) were identified between the radiosensitive and radioresistant groups. For model generation, a cohort of 59 patients with sarcomas from The Cancer Genome Atlas (TCGA) was used. DEGs of the responder and non-responder groups according to the radiotherapy-best response were identified. The overlapping DEGs between those from TCGA data and the STS cell line were subjected to linear regression to develop a formula, namely the STS-specific radioresistance index (STS-RRI), and its performance was compared with that of the previously established radiosensitivity index (RSI).</p><p><strong>Results: </strong>We selected thirteen overlapping DEGs and established STS-RRI using seven of them: STS-RRI = 1.5185 × MYO16-0.01575 × MYH11 + 3.900375 × KCTD16 + 0.105375 × SYNPO2-0.777375 × MYPN-0.849875 × PCSK6-0.700125 × LTK + 39.4635. Delong's test revealed that the STS-RRI performed better at stratifying responder and non-responder in TCGA cohort than the RSI (<i>p</i> = .002). The progression-free survival curves of the TCGA cohort were significantly discriminated by STS-RRI (<i>p</i> = .013) but not by RSI (<i>p</i> = .241).</p><p><strong>Conclusion: </strong>We developed the STS-RRI to predict the radioresistance of patients with STS in the TCGA dataset, showing a higher performance than RSI.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"283-291"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer activity of thymoquinone in non-small cell lung cancer and possible involvement of PPAR-<i>γ</i> pathway.","authors":"Mehmet Gurbilek, Cigdem D Deniz, Canan Eroglu Gunes, Ercan Kurar, Ismail Reisli, Muammer A Kursunel, Cemile Topcu, Mehmet Koc","doi":"10.1080/09553002.2025.2449953","DOIUrl":"10.1080/09553002.2025.2449953","url":null,"abstract":"<p><strong>Purpose: </strong>Thymoquinone (TQ) is an ingredient of <i>Nigella sativa</i> and Cisplatin (CDDP) is the most active chemotherapeutic agent in lung cancer. The objective of this study was to assess the anticancer effects of TQ in non-small cell lung cancer (NSCLC) cells, and its effect on the peroxisome proliferator-activated receptor gamma (PPAR-<i>γ</i>) pathway.</p><p><strong>Methods: </strong>Annexin-V FITC assay was used in the NCI-H460 cell line for apoptosis. The mRNA expression of PPAR-<i>γ</i>, P53, BCL-2, Retinoblastoma (Rb), Cyclin-D1, RELA, Tumor necrosis Factor alpha and in a dose-dependent manner TQ activated caspases 9, 8, 7, and 3 were examined using quantitative real-time reverse transcriptase polymerase chain reaction.</p><p><strong>Results: </strong>PPAR-<i>γ</i> protein levels elevated in all treatment groups, especially in the CDDP + TQ group as observed in mRNA results. In the CDDP + TQ + IR group, the reduction of NF-κB pathway, which provides survival and growth signaling, confirms the potential of this treatment in lung cancer treatment approach similar to p53, Rb, and PPAR-<i>γ</i> results. When the effect of treatment on the viability of NSCLC cells was assessed with flow cytometry analyzes, TQ alone supported death compared to control, cell viability also decreased in the CDDP or IR groups to which TQ was added.</p><p><strong>Conclusion: </strong>As a result, combined therapy of TQ, CDDP, and IR have been shown to increase apoptosis by sensitizing NSCLC cells to IR. These in vitro results are the basis because they demonstrate that it may be useful to include TQ in combined NSCLC cell treatments to reduce tumor progression.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"370-381"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143416388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical dosimetry of specific absorption rate of insects exposed to far-field radiofrequency electromagnetic fields.","authors":"Vera Jeladze, Tamar Nozadze, Besarion Partsvania, Arno Thielens, Levan Shoshiashvili, Teimuraz Gogoladze","doi":"10.1080/09553002.2024.2442693","DOIUrl":"10.1080/09553002.2024.2442693","url":null,"abstract":"<p><strong>Purpose: </strong>This paper reports a study of electromagnetic field (EMF) exposure of several adult insects: a ladybug, a honey bee worker, a wasp, and a mantis at frequencies ranging from 2.5 to 100 GHz. The purpose was to estimate the specific absorption rate (SAR) in insect tissues, including the brain, in order to predict the possible biological effects caused by EMF energy absorption.</p><p><strong>Method: </strong>Numerical dosimetry was executed using the finite-difference time-domain (FDTD) method. Insects were modeled as 3-tissue heterogeneous dielectric objects, including the cuticle, the inner tissue, and the brain tissue. The EMF source was modeled as sinusoidal plane waves at a single frequency (far-field exposure).</p><p><strong>Results: </strong>The whole-body averaged, tissue averaged, and 1 milligram SAR values were determined in insects for all considered frequencies for 10 different incident plane waves. SAR values were normalized to the incident power density of 1 mW/cm². Maximal EMF absorption in the inner and brain tissues was observed at 6, 12, and 25 GHz for the considered insects, except the brain tissue of a ladybug (max at 60 GHz).</p><p><strong>Conclusion: </strong>The paper presented the first estimation of the SAR for multiple insects over a wide range of RF frequencies using 3-tissue heterogenous insect 3D models created for this specific research. The selection of tissues' dielectric properties was validated. The obtained results showed that EMF energy absorption in insects highly depends on frequency, polarization, and insect morphology.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"327-340"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of long-term low-dose 2.45 GHz microwave electromagnetic radiation on vulnerable organs in humans: a retrospective study based on patients receiving microwave diathermies in the Department of Rehabilitation Medicine.","authors":"Zhi Zheng, Lihua Huang, Tao Shang, Yanhong Ma, Yiming Xu","doi":"10.1080/09553002.2025.2467671","DOIUrl":"10.1080/09553002.2025.2467671","url":null,"abstract":"<p><strong>Purpose: </strong>To retrospectively observe the effects of long-term low-dose 2.45 GHz microwave electromagnetic radiation on human vulnerable organs.</p><p><strong>Methods: </strong>This single-center, retrospective cohort study investigated patients who received long-term (20-100 times of microwave therapy within three months) low-dose (≤20 W) microwave therapy in our department five years ago. Patients were identified and followed up using a rehabilitation treatment management system. Adverse events or diseases that developed after microwave treatment were collected. Based on the number of microwave exposures, patients were divided into two groups: the multiple irradiation group (20-50 sessions) and the ultra-multiple irradiation group (50-100 sessions). The incidence of irreversible damage (ocular lesions, nervous system diseases, reproductive system diseases, cardiovascular system diseases, tumors, or early precancerous lesions) within five years after treatment completion was compared between the two groups.</p><p><strong>Results: </strong>A total of 113 valid cases were analyzed. Sixteen adverse events occurred, including two cases related to tumors, nine cases related to cardiovascular and cerebrovascular diseases, five cases related to metabolic diseases, and one case related to nervous system diseases. One patient had multiple conditions. There was no significant difference in the incidence of adverse events between the multiple irradiation group and the ultra-multiple irradiation group (p = .161, OR = 0.307, 95% CI 0.088-1.025). Logistics regression analysis revealed that the number of microwave treatments, treatment frequency per week, and patient gender was not a significant risk factor for adverse events (p = .100, OR = 0.972, 95% CI 0.938-1.006; p = .896, OR = 1.028, 95% CI 0.679-1.575; p = .960, OR = 1.039, 95% CI 0.212-4.609). Advanced age and obesity might be contributing factors for adverse events (p = .001, OR = 0.923, 95% CI 0.877-0.965; p = .002, OR = 0.064, 95% CI 0.009-0.348).</p><p><strong>Conclusions: </strong>Based on this study, receiving less than 100 sessions of low-dose microwave therapy within three months appears to be safe. Advanced age and obesity might increase the risk of adverse events. Due to the deviation that may be caused by the small sample size of this study, it is necessary to carry out prospective randomized controlled studies with larger samples in the future for further verification.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"607-613"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiofrequency radiation and Alzheimer's disease: harmful and therapeutic implications.","authors":"Hava Bektas, Suleyman Dasdag","doi":"10.1080/09553002.2025.2481854","DOIUrl":"10.1080/09553002.2025.2481854","url":null,"abstract":"<p><strong>Purpose: </strong>Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory loss and cognitive decline. The relationship between AD and radiofrequency (RF) radiation emitted by wireless devices remains under investigation. The aim of this review is to comprehensively explore the effects of RF radiation on AD by evaluating existing literature. This review used Web of Science, Scopus, and PubMed to find relevant studies on AD and RF radiation. This review evaluates a total of 81 studies, including animal models, human studies, and in vitro experiments, with results summarized in tables for clarity.</p><p><strong>Conclusion: </strong>Some studies suggest RF aggravates AD by increasing oxidative stress, impairing blood-brain barrier integrity, and promoting amyloid-beta deposition. Conversely, other studies indicate RF may have protective benefits, such as enhancing brain mitochondrial functions and reducing amyloid-beta levels. Understanding the RF-AD relationship, including parameters like frequency and exposure time, is crucial for therapeutic strategies. The studies reviewed highlight RF radiation's dual effects on AD, underscoring the need for a detailed approach. Further studies are required to clarify these effects and inform preventive and therapeutic measures.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"559-571"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blocking MSI2 alleviated radiation-induced pulmonary fibrosis through inhibiting epithelial-mesenchymal transition.","authors":"Shanlin Cai, Tingting Liu, Jianpeng Zhao, Ruling Liu, Liuhuan Yao, Rongbing Wei, Hu Liu, Jiaming Guo, Bailong Li","doi":"10.1080/09553002.2025.2451617","DOIUrl":"https://doi.org/10.1080/09553002.2025.2451617","url":null,"abstract":"<p><strong>Purpose: </strong>Ionizing radiation (IR) has been shown to induce epithelial-mesenchymal transition (EMT) of alveolar epithelial cells (AECs), which is a critical cause of radiation-induced pulmonary fibrosis (RIPF). In this study, we investigated the role and molecular mechanisms of musashi2 (MSI2), an RNA-binding protein, in IR-induced EMT of AECs for aiming at potential therapeutic strategies to prevent RIPF.</p><p><strong>Materials and methods: </strong>Changes in the expression levels of MSI2 and EMT markers (E-cadherin, N-cadherin, and Vimentin) induced by IR in AECs were detected by western blot (WB). Then, the effect of MSI2 on IR-induced EMT of AECs was investigated by observing morphological changes and detecting expression of MSI2 and EMT markers by WB and immunofluorescence (IF). RNA-Seq analysis, WB and RT-qPCR were used to identify the targets of MSI2.</p><p><strong>Results: </strong>We observed that IR could cause a significant increase of MSI2 protein expression, a down-regulation of E-cadherin and an up-regulation of Vimentin and N-cadherin in AECs (MLE-12 and RLE-6TN cells). We also revealed that MSI2 was involved in regulating the alteration of morphology and EMT-related markers in AECs after irradiation, suggesting the occurrence of EMT regulated by MSI2. Moreover, we found the mechanism of MSI2 participating in EMT by regulating the expression of transcription factor ZEB1, acting as a downstream target of MSI2 in IR-induced EMT of AECs.</p><p><strong>Conclusions: </strong>Our study unveils the critical role of MSI2 in IR-induced EMT of AECs and preliminarily elucidates its molecular mechanisms, providing new insights into the process of IR-induced pulmonary fibrosis.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":"101 5","pages":"475-486"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of multi-omics and benchmark dose modeling to support adverse outcome pathways.","authors":"Ngoc Q Vuong, Saadia Khilji, Andrew Williams, Nadine Adam, Danicia Flores, Kelly M Fulton, Isabel Baay, Susan M Twine, Matthew J Meier, Premkumari Kumarathasan, Ruth C Wilkins, Carole L Yauk, Vinita Chauhan","doi":"10.1080/09553002.2024.2442694","DOIUrl":"10.1080/09553002.2024.2442694","url":null,"abstract":"<p><strong>Background: </strong>Recent advancements in omics and benchmark dose (BMD) modeling have facilitated identifying the dose required for a predetermined change in a response (e.g. gene or protein change) that can be used to establish acceptable dose levels for hazardous exposures. Adverse Outcome Pathways (AOPs) describe the causal links between toxicants and adverse effects through key events (KEs). Integrating omics data within the AOP framework quantitatively links early molecular events to later phenotypic effects. In this study, we use omic-based BMD analyses in an in vitro blood model exposed to radiation to identify point of departure (POD) values across KEs to acute myeloid leukemia (www.aopwiki.org/aop/432).</p><p><strong>Methods: </strong>Isolated white blood cells were cultured and X-irradiated (1 Gy/minute, 0-6 Gy). Transcriptomic and proteomic changes were assessed 24 h post-exposure. BMD modeling was applied and significantly perturbed genes/proteins and pathways were identified. Those pathways relevant to KEs outlined in AOP 432 were grouped and a POD was determined.</p><p><strong>Results: </strong>BMD modeling identified 1294 genes and 167 proteins with median BMD lower confident limit (BMD) values of 1.35 and 0.32 Gy, respectively. Pathway analysis identified biological processes related to DNA damage/repair, oxidative stress, cell cycle regulation, immune responses, and cancer development. These findings aligned with the KEs in AOP 432. The BMDL values of canonical pathways associated with these KEs were generally below 0.5 Gy with specific genes (e.g. GADD45A) displaying BMDLs <0.05 Gy.</p><p><strong>Conclusions: </strong>This work provides insights into predictive radiation induced mechanisms and associated dose of activity that can be taken into consideration for low dose (< 0.1 Gy) risk analysis.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"240-253"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combination of microwave hyperthermia with TIPE2 impedes the growth of orthotopic colon cancer.","authors":"Qingqing Yu, Lingdi Li, Weixing Mo, Linfang Zhao, Lidan Zhang, Ke Zhang, Rongjun Tang","doi":"10.1080/09553002.2024.2435324","DOIUrl":"10.1080/09553002.2024.2435324","url":null,"abstract":"<p><strong>Background: </strong>Colon cancer (CC) is the main fatal disease of humans. Microwave hyperthermia (MH) is an adjuvant therapy for diverse cancers. Tumor necrosis factor-α induced protein-8-like 2 (TIPE2) is a tumor suppressor. However, the effect of MH combined with TIPE2 on CC remains unclear.</p><p><strong>Methods: </strong>The orthotopic CC mouse model was constructed by mouse CC CT26-Luc cells, and mice were randomized into control, model (CT26-Luc), CT26-Luc + Vector, CT26-Luc + TIPE2, CT26-Luc + MH, and CT26-Luc + MH+TIPE2 groups (<i>n</i> = 6). Tumor growth pretreatment and post-treatment by <i>in vivo</i> fluorescence image analysis was detected. TIPE2 expression and cell transfection efficiency was detected by qRT-PCR and western blot. The pathological changes by HE staining, apoptosis by TUNEL staining, serum inflammatory factors by ELISA, TIPE2 expression by immunohistochemistry, and NF-κB signaling by western blot was performed.</p><p><strong>Results: </strong>Paracancerous tissues showed higher TIPE2 expression than in CC tissues. CT26-Luc + TIPE2, CT26-Luc + MH, and CT26-Luc + MH+TIPE2 groups reduced tumor growth, tumor cell infiltration, and increased apoptosis. CT26-Luc + TIPE2 group had lower NF-κB, TNF-α, IL-1β, IL-6, p-p65, and p-IKK expression, and elevated TIPE2 and IkB expression, which was reversed by CT26-Luc + MH group. Moreover, CT26-Luc+MH+TIPE2 group showed opposite effects on the above factor expression of CT26-Luc+MH group.</p><p><strong>Conclusions: </strong>Combination of MH with TIPE2 could impede CC tumor growth, providing scientific bases for its clinical application in CC treatment.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"205-214"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro regeneration and optimization of physical and chemical mutagenesis protocol in tuberose (<i>Agave amica</i> (Medik.) Thiede & Govaerts) cv. 'Arka Vaibhav'.","authors":"Mahananda Patil, Thangaraj Usha Bharathi, T R Usharani, M R Rohini, Rajiv Kumar, Balaji S Kulkarni, Keerthi M C","doi":"10.1080/09553002.2024.2447511","DOIUrl":"10.1080/09553002.2024.2447511","url":null,"abstract":"<p><strong>Purpose: </strong>Tuberose (<i>Agave amica</i> [Medik.]) is a vegetatively propagated commercial flower crop with limited genetic variability. Crossing barriers prevailing in tuberose necessitates modern breeding techniques like in vitro mutagenesis to generate variability. Hence, this study aimed to establish an efficient in vitro regeneration protocol for the rapid multiplication of tuberose and optimize the method for in vitro mutagenesis using the terminal stem scale as the explant.</p><p><strong>Results: </strong>MS medium supplemented with 17.74 µM benzyl aminopurine) (BAP) and 0.57 µM indole-3-acetic acid (IAA) showed the maximum number of multiple shoots (5.0), with optimal shoot length (6.77 cm) and number of leaves (6.07). The shoots formed maximum rooting (99.44%) in MS medium supplemented with 4.90 µM indole-3-butyric acid, with an average of 26.89 roots per shoot. In vitro mutagenesis attempted physically <i>via</i> gamma irradiation led to an LD_{25, 50, 75} values of 13.21, 20.81, 32.79 Gy, respectively. The incorporation of ethyl methane sulfonate (EMS) into the culture media at a concentration of 0.08%, 0.13%, and 0.21% effectively resulted in LD_{25, 50, 75}, respectively. Pretreating explants with 0.13% EMS for 15 min, 0.18% EMS for 30 min, 0.14% EMS for 45, and 0.11% EMS for 60 min were optimal for achieving 50% survival and plant regeneration using the regeneration protocol described above.</p><p><strong>Conclusion: </strong>The regeneration protocol and optimized mutagen dose for in vitro mutagenesis developed in this study can be utilized for rapid multiplication of the cultivar and as a tool in genetic improvement programs aimed at inducing variability for commercially significant traits in tuberose.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"398-410"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143034891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LBDNet inter-laboratory comparison at high doses of ionizing radiation using the dicentric plus caffeine assay.","authors":"Ivonne Romero, Tania Mandina Cardoso, Mariana Cabitto, Mayra Deminge, Julieta Fernandez Rearte, Hernan Vaquero, Fabiana Farias de Lima, Mariana Esposito Mendes, Laís Melo Silva, Erika Flavia Lafuente-Álvarez, Ana Rada-Tarifa, Valentina Verdejo, Analia Radl, Nicolás Saavedra, Mauricio Santibañez, Nelson Brenes Obando, Fabio Andrés Chaves-Campos, Fernando Ortíz, Luisa Valle, Jorge Ernesto González Mesa, Angela Bastidas, Gabriela Muñoz-Velástegui, Carolina Arceo-Maldonado, Yolanda Citlali Guerrero-Carbajal, Sara Aguilar-Coronel, Norma Monjagata, Marco Espinoza-Zevallos, Wilner Martínez-López, Burix Mechoso, María Vittoria Di Tomaso, Aida Falcón de Vargas, Omar García Lima","doi":"10.1080/09553002.2025.2494554","DOIUrl":"10.1080/09553002.2025.2494554","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the performance of the LBDNet laboratories in estimating dose over 5 Gy of ionizing radiation using the dicentric chromosome plus caffeine assay.</p><p><strong>Materials and methods: </strong>Dose-response curve fitting: Peripheral blood was irradiated <i>in vitro</i> between 5 and 25 Gy. Then, the DC plus caffeine assay was carried out. Thirteen laboratories received and analyzed metaphase images. The linear dose-response curve was fitted for each laboratory. Dose estimation was performed analyzing coded metaphase images from three different irradiated samples (7.5, 15, 20 Gy) and using the fitted curve from every laboratory.</p><p><strong>Results: </strong>The dose estimation accuracy was within the expected dose ranges. The 76.9%, 84.6% and 69.2% of the estimated doses fell into the ± 20% of the true radiation dose. The 92.3%, 92.3%, and 61.5% of the 95% of the confidence interval of the estimated doses included the true radiation dose. The trueness was 0.9%, 4.4% and 9.6%. The Coefficients of Variation of the estimated doses were 14.5%, 16.1% and 17.8%. Results from only one laboratory were deemed questionable for dose estimation, based on the <i>Z-score</i> derived from robust methods.</p><p><strong>Conclusion: </strong>The intercomparison study yielded satisfactory results; however, dose estimation accuracy tended to decrease, and variability between laboratory results increased as the dose level rose.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"636-651"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}