LBDNet inter-laboratory comparison at high doses of ionizing radiation using the dicentric plus caffeine assay.

International journal of radiation biology Pub Date : 2025-01-01 Epub Date: 2025-05-05 DOI:10.1080/09553002.2025.2494554

Ivonne Romero, Tania Mandina Cardoso, Mariana Cabitto, Mayra Deminge, Julieta Fernandez Rearte, Hernan Vaquero, Fabiana Farias de Lima, Mariana Esposito Mendes, Laís Melo Silva, Erika Flavia Lafuente-Álvarez, Ana Rada-Tarifa, Valentina Verdejo, Analia Radl, Nicolás Saavedra, Mauricio Santibañez, Nelson Brenes Obando, Fabio Andrés Chaves-Campos, Fernando Ortíz, Luisa Valle, Jorge Ernesto González Mesa, Angela Bastidas, Gabriela Muñoz-Velástegui, Carolina Arceo-Maldonado, Yolanda Citlali Guerrero-Carbajal, Sara Aguilar-Coronel, Norma Monjagata, Marco Espinoza-Zevallos, Wilner Martínez-López, Burix Mechoso, María Vittoria Di Tomaso, Aida Falcón de Vargas, Omar García Lima

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Abstract

Purpose: To assess the performance of the LBDNet laboratories in estimating dose over 5 Gy of ionizing radiation using the dicentric chromosome plus caffeine assay.

Materials and methods: Dose-response curve fitting: Peripheral blood was irradiated in vitro between 5 and 25 Gy. Then, the DC plus caffeine assay was carried out. Thirteen laboratories received and analyzed metaphase images. The linear dose-response curve was fitted for each laboratory. Dose estimation was performed analyzing coded metaphase images from three different irradiated samples (7.5, 15, 20 Gy) and using the fitted curve from every laboratory.

Results: The dose estimation accuracy was within the expected dose ranges. The 76.9%, 84.6% and 69.2% of the estimated doses fell into the ± 20% of the true radiation dose. The 92.3%, 92.3%, and 61.5% of the 95% of the confidence interval of the estimated doses included the true radiation dose. The trueness was 0.9%, 4.4% and 9.6%. The Coefficients of Variation of the estimated doses were 14.5%, 16.1% and 17.8%. Results from only one laboratory were deemed questionable for dose estimation, based on the Z-score derived from robust methods.

Conclusion: The intercomparison study yielded satisfactory results; however, dose estimation accuracy tended to decrease, and variability between laboratory results increased as the dose level rose.

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LBDNet实验室间在高剂量电离辐射下使用双中心加咖啡因测定的比较。

目的：评估LBDNet实验室使用双中心染色体加咖啡因测定法估算超过5gy电离辐射剂量的性能。材料与方法：剂量-反应曲线拟合：体外5 ~ 25 Gy辐照外周血。然后，进行DC加咖啡因实验。13个实验室接收并分析了中期图像。拟合了各实验室的线性剂量响应曲线。对三种不同辐照样品（7.5、15、20 Gy）的编码中期图像进行剂量估计，并使用每个实验室的拟合曲线。结果：剂量估算精度在预期剂量范围内。分别有76.9%、84.6%和69.2%的估计剂量落在真实辐射剂量的±20%范围内。估计剂量95%置信区间的92.3%、92.3%和61.5%包含了真实辐射剂量。正确率分别为0.9%、4.4%和9.6%。估计剂量的变异系数分别为14.5%、16.1%和17.8%。根据稳健方法得出的z分数，只有一个实验室的结果被认为对剂量估计有疑问。结论：比较研究取得满意结果；然而，剂量估计的准确性趋于降低，实验室结果之间的可变性随着剂量水平的升高而增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of radiation biology

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