International journal of radiation biology最新文献

{"title":"Influence of stromal neural crest progenitor cells on neuroblastoma radioresistance.","authors":"Carlos Huertas-Castaño, Laura Martínez-López, Patricia Cabrera-Roldán, Nuria Pastor, Juan Carlos Mateos, Santiago Mateos, Ricardo Pardal, Inmaculada Domínguez, Manuel Luis Orta","doi":"10.1080/09553002.2024.2440865","DOIUrl":"https://doi.org/10.1080/09553002.2024.2440865","url":null,"abstract":"<p><strong>Purpose: </strong>A substantial proportion of children with high risk Neuroblastoma die within the first 5 years post-diagnosis despite the complex treatment applied. In the recent years, tumor environment has been revealed as key factor for cancer treatment efficacy. In this sense, non-tumorigenic Neural Crest progenitor cells from high risk patients, have been described as part of Neuroblastoma stroma, promoting tumor growth and contributing to mesenchyme formation. In this paper we wanted to study the radiobiological behavior of these cells (NB14t) and how they influence the growth of tumorigenic neuroblasts after radiotherapy.</p><p><strong>Materials and methods: </strong>To achieve our aim, we employed a wide list of methods either using NB14t cells as well as commercial NB cells. We have analyzed viability, survival, cell cyle profiles and differentiation. In addition, cocultured experiments were performed to monitor the influence of stroma cells to tumorigenic neuroblasts.</p><p><strong>Results: </strong>We found that stromal progenitor cells showed an extraordinary radio-resistance either cultured in attached or suspension conditions. In good agreement, we found an enhanced repair of irradiation-induced DNA lesions as compared with commercial cell lines. In addition, according to our data these cells differentiate into a Cancer Associated Fibroblasts (CAFs)-like phenotype, hence contributing to the formation of mesenchymal stroma enhancing the growth of tumor cells after irradiation.</p><p><strong>Conclusion: </strong>Our data show that neural progenitor cells from high risk NB stroma are radio-resistant and promote cancer growth after irradiation. This paper can help to understand the complex cell relationships within a tumor that will determine patient prognosis after radiotherapy.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose threshold for residual γH2AX, 53BP1, pATM and p-p53 (Ser-15) foci in X-ray irradiated human fibroblasts.","authors":"Andrey Osipov, Anna Chigasova, Oleg Belov, Elizaveta Yashkina, Maxim Ignatov, Yuriy Fedotov, Natalia Vorobyeva, Andreyan N Osipov","doi":"10.1080/09553002.2024.2445581","DOIUrl":"https://doi.org/10.1080/09553002.2024.2445581","url":null,"abstract":"<p><strong>Background: </strong>Enumeration of residual DNA repair foci 24 hours or more after exposure to ionizing radiation (IR) is often used to assess the efficiency of DNA double-strand break repair. However, the relationship between the number of residual foci in irradiated cells and the radiation dose is still poorly understood. The aim of this work was to investigate the dose responses for residual DNA repair foci in normal human fibroblasts after X-ray exposure in the absorbed dose range from 0.1 to 5 Gy.</p><p><strong>Materials and methods: </strong>Fibroblasts were irradiated using a X-ray unit at an absorbed dose rate of 0.2 Gy/min. Irradiated cells were incubated for 0.5, 24, 48 and 72 h. Immunofluorescence visualized γH2AX, 53BP1, pATM and p-p53 (Ser-15) foci were enumerated using DARFI software and by manual scoring. Additionally, clonogenic survival analysis was performed.</p><p><strong>Results: </strong>The data analysis performed with the hockey stick model showed the presence of a dose threshold for the residual foci of all proteins studied. The estimated threshold doses are close to the quasi-threshold dose (Dq = 0.99 ± 0.09 Gy) calculated from the cell survival curve.</p><p><strong>Conclusion: </strong>The excellent agreement between the calculated values of the threshold dose and Dq in irradiated fibroblasts proves that residual foci are sites, where cells are still attempting to repair potentially lethal DNA damage.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examination of immunohistochemical of the effects of flattened and unflattened radiotherapy beams in nude mice breast cancer xenografts.","authors":"Serhat Aras, Seyma Ozkanli, Engin Sumer, Tugba Kul Koprulu, Mustafa Efendioglu","doi":"10.1080/09553002.2024.2445582","DOIUrl":"https://doi.org/10.1080/09553002.2024.2445582","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to investigate the radiobiological effects underlying the inhibition of breast cancer (BCa) following radiotherapy in nude mice models, and to evaluate the impact of changes in immunohistochemical parameters induced by FF and FFF beams.</p><p><strong>Materials and methods: </strong>The study included thirty-six adult nude mouse models, which were randomly assigned to five groups: control (G1), breast cancer (BCa) (G2), FF-400 MU/min (G3), FFF-1100 MU/min (G4), and FFF-1800 MU/min (G5). The control group received neither radiation nor treatment, while the BCa group had a cancer model without radiation. The BCa models were subjected to a single dose of 20 Gy of radiotherapy at varying dose rates. Twenty days after the implantation of the MCF-7 cancer cell line, the nude mice were irradiated and sacrificed 48 h later for ER, PR, HER-2, Ki-67, CD-133, Caspase-3, APAF-1, NOS-2 and NOS-3 IHC analysis.</p><p><strong>Results: </strong>A statistically significant decrease in IHC staining values for ER, Ki-67 and NOS-2 was observed in the FF-400 MU/min, FFF-1100 MU/min and FFF-1800 MU/min groups due to radiotherapy compared to the BCa group. The FFF beams demonstrated superior efficacy in the treatment of BCa. The significant differences in Caspase-3 and APAF-1 levels were found between BCa and control groups, while CD-133, NOS-3, HER-2, and PR staining showed no differences between groups.</p><p><strong>Conclusions: </strong>It was concluded that FFF beam was more effective than FF beam for BCa, especially on ER, Ki-67 and NOS-2 IHC parameters.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical dosimetry of specific absorption rate of insects exposed to far-field radiofrequency electromagnetic fields.","authors":"Vera Jeladze, Tamar Nozadze, Besarion Partsvania, Arno Thielens, Levan Shoshiashvili, Teimuraz Gogoladze","doi":"10.1080/09553002.2024.2442693","DOIUrl":"https://doi.org/10.1080/09553002.2024.2442693","url":null,"abstract":"<p><strong>Purpose: </strong>This paper reports a study of electromagnetic field (EMF) exposure of several adult insects: a ladybug, a honey bee worker, a wasp, and a mantis at frequencies ranging from 2.5 to 100 GHz. The purpose was to estimate the specific absorption rate (SAR) in insect tissues, including the brain, in order to predict the possible biological effects caused by EMF energy absorption.</p><p><strong>Method: </strong>Numerical dosimetry was executed using the finite-difference time-domain (FDTD) method. Insects were modeled as 3-tissue heterogeneous dielectric objects, including the cuticle, the inner tissue, and the brain tissue. The EMF source was modeled as sinusoidal plane waves at a single frequency (far-field exposure).</p><p><strong>Results: </strong>The whole-body averaged, tissue averaged, and 1 milligram SAR values were determined in insects for all considered frequencies for 10 different incident plane waves. SAR values were normalized to the incident power density of 1 mW/cm². Maximal EMF absorption in the inner and brain tissues was observed at 6, 12, and 25 GHz for the considered insects, except the brain tissue of a ladybug (max at 60 GHz).</p><p><strong>Conclusion: </strong>The paper presented the first estimation of the SAR for multiple insects over a wide range of RF frequencies using 3-tissue heterogenous insect 3D models created for this specific research. The selection of tissues' dielectric properties was validated. The obtained results showed that EMF energy absorption in insects highly depends on frequency, polarization, and insect morphology.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of multi-omics and benchmark dose modeling to support adverse outcome pathways.","authors":"Ngoc Q Vuong, Saadia Khilji, Andrew Williams, Nadine Adam, Danicia Flores, Kelly M Fulton, Isabel Baay, Susan M Twine, Matthew J Meier, Premkumari Kumarathasan, Ruth C Wilkins, Carole L Yauk, Vinita Chauhan","doi":"10.1080/09553002.2024.2442694","DOIUrl":"https://doi.org/10.1080/09553002.2024.2442694","url":null,"abstract":"<p><strong>Background: </strong>Recent advancements in omics and benchmark dose (BMD) modeling have facilitated identifying the dose required for a predetermined change in a response (e.g. gene or protein change) that can be used to establish acceptable dose levels for hazardous exposures. Adverse Outcome Pathways (AOPs) describe the causal links between toxicants and adverse effects through key events (KEs). Integrating omics data within the AOP framework quantitatively links early molecular events to later phenotypic effects. In this study, we use omic-based BMD analyses in an in vitro blood model exposed to radiation to identify point of departure (POD) values across KEs to acute myeloid leukemia (www.aopwiki.org/aop/432).</p><p><strong>Methods: </strong>Isolated white blood cells were cultured and X-irradiated (1 Gy/minute, 0-6 Gy). Transcriptomic and proteomic changes were assessed 24 h post-exposure. BMD modeling was applied and significantly perturbed genes/proteins and pathways were identified. Those pathways relevant to KEs outlined in AOP 432 were grouped and a POD was determined.</p><p><strong>Results: </strong>BMD modeling identified 1294 genes and 167 proteins with median BMD lower confident limit (BMD) values of 1.35 and 0.32 Gy, respectively. Pathway analysis identified biological processes related to DNA damage/repair, oxidative stress, cell cycle regulation, immune responses, and cancer development. These findings aligned with the KEs in AOP 432. The BMDL values of canonical pathways associated with these KEs were generally below 0.5 Gy with specific genes (e.g. GADD45A) displaying BMDLs <0.05 Gy.</p><p><strong>Conclusions: </strong>This work provides insights into predictive radiation induced mechanisms and associated dose of activity that can be taken into consideration for low dose (< 0.1 Gy) risk analysis.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the optimal gamma irradiation dose for developing novel cowpea (<i>Vigna unguiculata</i>) genotypes.","authors":"Sara Diallo, François Abaye Badiane, Mame Diarra Gueye, Made Diouf, Diaga Diouf","doi":"10.1080/09553002.2024.2440849","DOIUrl":"https://doi.org/10.1080/09553002.2024.2440849","url":null,"abstract":"<p><strong>Purpose: </strong>Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) is a major legume crops for human consumption and livestock feed in tropical regions. Although its importance, the crop's production is subjected to numerous constraints, raising the need to develop outstanding genotypes. In this line, this study assesses the effects of gamma irradiation doses on cowpea genotypes to determine the LD50 and its effects on agro-morphological parameters.</p><p><strong>Materials and methods: </strong>Healthy dry seeds of three cowpea genotypes, Bambey 21, Me51-M4-39M9, and Ndout violet pods (VP), were exposed to four doses of gamma-rays 0, 200, 300, and 400 Gy. Qualitative and quantitative parameters were evaluated since germination, and a two-way analysis of variance (ANOVA) was performed on the means of quantitative traits using R software.</p><p><strong>Results: </strong>The results revealed that the LD50 on seed germination and plant survival ranged from 579 and 446.25 Gy, in Me51-M4-39M9, respectively. These values varied significantly among genotypes and revealed that low doses of gamma irradiation stimulated germination speed and had a positive effect on the early flowering of Ndout VP. Higher doses of gamma irradiation induced more severe mutations, causing visible effects such as changes in leaf color (albino, xantha, viridis, and variegation) and phyllotaxis. The frequency of chlorophyll mutants induced by gamma irradiation was found to be dose-genotype/dependent, with Bambey 21 being the most sensitive variety. Lower doses induced desirable mutations such as stem pigmentation and seed hilum coloration on Bambey 21 and Me51-M4-39M9. It has also had a positive impact on seedling height and leaf number in Bambey 21 and Ndout VP. However, high irradiation doses lead to a significant reduction in certain quantitative traits, such as plant height (PH), leaf length (LL), leaf width (LW), pod width (PWD), pod weight (PW), seed width (SWD), and seed weight. Analysis of the phenotypic performance of quantitative traits allowed us to cluster the four doses by genotype into three groups.</p><p><strong>Conclusions: </strong>The optimum dose of gamma-irradiation in cowpea mutation breeding is genotype-dependent. The effects of gamma-irradiation on these traits and their relationships are highly dependent on the specific crop and genotype. Further research is needed to understand these effects underlying mechanisms and develop crop improvement strategies using gamma irradiation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collaborative activities in a biological dosimetry network for radiation emergencies in South Korea.","authors":"Yang Hee Lee, Su San Yang, Hyo Jin Yoon, Hwa Young Kim, Soon Woo Kwon, Soo Kyung Jeong, Su Jung Oh, Seong-Hoon Park, Younghyun Lee, Ki Moon Seong","doi":"10.1080/09553002.2024.2447506","DOIUrl":"https://doi.org/10.1080/09553002.2024.2447506","url":null,"abstract":"<p><strong>Purpose: </strong>Biological dosimetry is an essential analytic method to estimate the absorbed radiation dose in the human body by measuring changes in biomolecules after radiation exposure. Joint response in a network to mass-casualty radiation incidents is one way to overcome the limitations of biological dosimetry, sharing the workload among laboratories. This study aimed to investigate the current performance, collaborative activities and technical advances of the Korea biodosimetry network (K-BioDos), and suggest the future directions toward successful joint response.</p><p><strong>Materials and methods: </strong>A survey was performed to investigate the capacities of each laboratory and their expectations for the K-BioDos network. We summarized the capacities, expectations and technical advances of K-BioDos members. Based on the results, in-depth discussion was carried out to determine the future plan and activities of K-BioDos.</p><p><strong>Results: </strong>K-BioDos has grown to six laboratories since its establishment with three functional laboratories of biological dosimetry in South Korea. We constructed long-term strategy according the survey results, and performed various activities for enhanced biological dosimetry capabilities - including intercomparison exercises, education, and resource sharing. Through these active collaborations we achieved harmonization of biodosimetry protocols and technical improvement such as better image quality.</p><p><strong>Conclusions: </strong>K-BioDos network performed various activities for joint response and constructed long-term plans, considering the expectations and feedbacks of members. K-BioDos continue to support members to establish and develop biodosimetry tools. These efforts and findings could serve as a fundamental guide for coordinated network responses in the event of large-scale radiological disaster.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An examination of the dose rate effect in mice assuming that the carcinogenic effect of radiation is life shortening resulting from a tissue reaction.","authors":"Nori Nakamura","doi":"10.1080/09553002.2024.2442690","DOIUrl":"https://doi.org/10.1080/09553002.2024.2442690","url":null,"abstract":"<p><strong>Purpose: </strong>Radiation exposures do not seem to increase the proportion of mice dying from tumors, but rather cause a shift in the appearance of spontaneous cancers, allowing them to appear earlier, and hence produce a life shortening effect. Then, it was possible to estimate the effect of the dose rate on the carcinogenic effects of radiation using life shortening effects as a measure.</p><p><strong>Conclusion: </strong>The dose response for the induction of life shortening was linear under acute exposure conditions, which indicates that the response under chronic exposure conditions is also likely to be linear, and hence the dose rate factor (DRF) would be constant throughout the dose. Furthermore, the life shortening effect decreased sharply with an increase in age at exposure. To separate the dose rate effect from the effects of age under long-term exposure conditions, a thought experiment was designed which consisted of 8 repeated exposures to an acute 1 Gy dose at intervals of 50 days with an assumption that the effect is additive, and the results were compared with those observed in a chronic continuous exposure experiment (20 mGy per day for 400 days, for a total of 8 Gy: Tanaka et al. 2003). The results showed 211 days of life shortening in the former and 120 days in the latter, which provided a DRF of 1.8 (211/120). If one assumes that a tissue reaction is the primary cause of radiation carcinogenesis, the contrasting two concepts, radiation hormesis and linear-non-threshold model at low doses, would become compatible.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"γ-radiation induced reduction in antinutrients of buckwheat (<i>Fagopryum esculentum</i> Moench) seeds and leaves.","authors":"Kuldip Chandra Verma, Kumkum Giri, Sanjay Kumar Verma, Pawanesh Tamta, Nidhi Joshi","doi":"10.1080/09553002.2024.2445580","DOIUrl":"https://doi.org/10.1080/09553002.2024.2445580","url":null,"abstract":"<p><strong>Purpose: </strong>Buckwheat, a dicotyledonous crop of Polygonaceae family, is known for its nutritional value and adaptability to adverse climates. Local people reported that prolonged consumption of buckwheat seeds and leaves causes numbness and gastrointestinal problems. The present study was conducted to observe the impact of different doses of γ-radiations on phytoconstituents of buckwheat seeds and leaves, to make them nutritionally superior.</p><p><strong>Materials and methods: </strong>Buckwheat seeds were treated with 5, 10, 15 and 20 kGy doses of γ-radiations and grown in an experimental farm. Various phytoconstituents in seeds and leaves were analyzed.</p><p><strong>Results: </strong>The antioxidant, phenol, flavonoid, β-carotene, iron, calcium, lysine and arginine were increased significantly (<5%) with increasing doses of γ-radiations up to 10 kGy, whereas, anti-nutrients (tannin, phytic acid and oxalate) decreased significantly (<5%). γ-radiation @ 10 kGy is the best for the enhancement of phytoconstituents in buckwheat seeds from a nutrition point of view. Phytoconstituents in buckwheat leaves and irradiated seed progeny were positively co-related with M1 seeds.</p><p><strong>Conclusions: </strong>It can be concluded that the buckwheat seeds treated with a 10 kGy dose of γ-radiation are the best to produce green leaves as hara saag, and progeny seeds for preparation of flour. However, superior mutant selection and effect of by-products from γ-irradiated buckwheat seeds is the thrust area of future research.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening model in <i>Caenorhabditis elegans</i> for radioprotective natural products.","authors":"Wenxi Yang, Zhihui Li, Xiaojuan Chen, Shuang Wu, Si Liu, Lan Yao, Jie Zhang, Haizhen Liang, Juan Song, Baiping Ma","doi":"10.1080/09553002.2024.2445577","DOIUrl":"https://doi.org/10.1080/09553002.2024.2445577","url":null,"abstract":"<p><strong>Purpose: </strong>Ionizing radiation (IR) could induce damage such as DNA damage and oxidative stress. Natural products, like tea, have been demonstrated potential in mitigating these damages. However, the lack of efficient and rapid screening methods for natural products hinders their widespread application. To address this challenge, this study utilized <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) as an <i>in vivo</i> model to investigate radioprotective natural products.</p><p><strong>Methods: </strong>L1 stage <i>C. elegans</i> were exposed to X-rays or ⁶⁰Co γ-rays at varying dosages (20, 50, and 100 Gy), then the growth, reproduction, and lifespan of the nematodes were observed. Different culture and sample-administered modes were tested. Known radioprotective agents, including Amifostine (WR2721), <i>Lycium barbarum</i> extract (LBE), and <i>Trillium tschonoskii</i> fraction (TTF), served as positive controls to validate the reliability of the model. The radioprotective activity of teas with different fermentation degrees was compared based on this screening model.</p><p><strong>Results: </strong>A screening model in <i>C. elegans</i> was established by X-rays at 20 Gy. An appropriate sample-administrated approach was investigated, which involves adding the sample to the nematode growth medium (NGM) agar covered with inactivated <i>Escherichia coli</i> 2 h before irradiation. The known radioprotective agents (WR2721, LBE, and TTF) validated that the model is stable. Our results of the model application revealed that teas with lower fermentation levels, such as green tea and oolong tea, particularly the <i>n</i>-butanol and ethyl acetate fractions from oolong tea, exhibited significant radioprotective activity.</p><p><strong>Conclusions: </strong>This study presents an effective <i>in vivo</i> approach for the initial screening of radioprotective natural products.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}