International journal of radiation biology最新文献

{"title":"Associations of hematological and biochemical parameters with radon exposure among male miners in China.","authors":"Xiaomin Guo, Gang Gao, Jianlei Ruan, Shiyue Cui, Chunnan Piao, Xiaoliang Li, Jia Zhang, Pinhua Zhang, Jianxiang Liu","doi":"10.1080/09553002.2026.2745882","DOIUrl":"https://doi.org/10.1080/09553002.2026.2745882","url":null,"abstract":"<p><strong>Purpose: </strong>Radon is a radioactive gas that produces a large amount of alpha particles during its decay process and a well-recognized health hazard, particularly an established risk factor for lung cancer in high-risk populations such as miners. Although hematological and biochemical parameters are useful indicators of human health status, the impact of radon exposure on these parameters among male miners remains unclear.</p><p><strong>Methods: </strong>The participants of this study are 283 male miners in a tin mine in China. A targeted research design was adopted to conduct a questionnaire survey, physical examination, blood sample analysis coupled with radon concentration measurements, statistical analysis was performed to investigate the impact of varying levels of radon exposure on hematological and biochemical parameters among the participants.</p><p><strong>Results: </strong>Mean platelet volume (MPV), plateletcrit (PCT), and platelet-large cell ratio (P-LCR) decreased significantly across the working-level month (WLM) groups (Bonferroni-corrected <i>P</i> < 0.003), as did albumin (ALB) and the albumin-to-globulin (A/G) ratio (Bonferroni-corrected <i>P</i> < 0.0045). In contrast, urea and aspartate aminotransferase (AST) increased significantly (Bonferroni-corrected <i>P</i> < 0.0045), whereas platelet count did not differ significantly among groups. Multiple linear regression confirmed WLM as an independent predictor for MPV (<i>β</i>= -0.282, <i>P</i> < 0.0001), PCT (<i>β</i>= -0.165, <i>P</i> = 0.010), P-LCR (<i>β</i>= -0.206, <i>P</i> = 0.002), urea (<i>β</i> = 0.178, <i>P</i> = 0.008), and platelet distribution width (<i>β</i> = 0.184, <i>P</i> = 0.006). All values remained within clinically normal reference ranges.</p><p><strong>Conclusion: </strong>Cumulative occupational radon exposure was independently associated with decreased platelet volume indices and elevated urea levels in miners, suggesting subclinical hematological changes and altered renal-related parameters. MPV may serve as a candidate indicator requiring further validation of radiation-related hematopoietic alterations. These findings contribute to occupational health surveillance frameworks for populations chronically exposed to radon in mining environments.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":2.4,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149248104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic and immunogenic signals of small extracellular vesicles from melanoma cells exposed to auger targeted radionuclide therapy.","authors":"Joshua Karam, Sarah Braga-Cohen, Alain Mangé, Marie Morille, Julie Constanzo, Jean-Pierre Pouget","doi":"10.1080/09553002.2026.2733680","DOIUrl":"https://doi.org/10.1080/09553002.2026.2733680","url":null,"abstract":"<p><strong>Purpose: </strong>Targeted radionuclide therapy (TRT) using beta emitters is an established tool for eradicating tumor cells; however, attention is increasingly focused on high-linear energy transfer particles, such as Auger electrons emitted during Auger TRT, due to their ability to generate bystander and immunological responses. In this context, we investigated whether extracellular vesicles (EVs) from cancer cells exposed to Auger TRT contribute to these responses.</p><p><strong>Materials and methods: </strong>EVs were purified from B16F10 melanoma cells exposed to TRT with the Auger emitter ¹²⁵I-TA99 (TRT-EVs). The involvement of immunogenic cell death in TRT effect was investigated using immunocompetent and nude mice. TRT-EVs cytotoxic effect on recipient (non-exposed) B16F10 cells was investigated using clonogenic survival, γ-H2AX focus formation, apoptosis assays and EVs were characterized by proteomic analysis.</p><p><strong>Results: </strong>In vivo data showing that TRT with ¹²⁵I-TA99 significantly delayed tumor growth in immunocompetent C57BL/6J mice, but not in T-cell-deficient athymic nude mice. Consistently, TRT-exposed cells exhibited enhanced extracellular ATP release and sequential cGAS-STING pathway activation. In vitro, TRT-EVs reduced recipient B16F10 cell clonogenic survival by 72 ± 15% and induced DNA double-strand breaks and micronuclei, but increased apoptosis only slightly. Proteomic analysis of TRT-EVs revealed an enrichment in proteins involved in immunogenic cell death and immune activation.</p><p><strong>Conclusions: </strong>Overall, this study provides evidence that TRT-EVs contribute to cytotoxicity by delivering toxic and immunogenic signals that drive a T-cell-dependent antitumor response. Investigations are currently underway to identify the specific proteins that mediate this immune activation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-13"},"PeriodicalIF":2.4,"publicationDate":"2026-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149248081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of proton LET and ultrahigh dose-rate on the structural integrity of DNA in solution.","authors":"Amitava Adhikary, Daniel M Sforza, Lingshu Yin, Fred Bunz, Mohammad Rezaee","doi":"10.1080/09553002.2026.2747690","DOIUrl":"https://doi.org/10.1080/09553002.2026.2747690","url":null,"abstract":"<p><strong>Purpose: </strong>Ultra-high dose-rate (UHDR) irradiation reduces normal tissue toxicity while maintaining tumor control under isodose conditions with respect to conventional dose-rate (CONV) irradiation (the FLASH effect). But its underlying physicochemical mechanism remains highly debated. This study evaluates whether radical-radical recombination can explain this sparing without invoking bulk oxygen depletion by decoupling the roles of track structure (linear energy transfer, LET) and particle fluence and velocity on free-radical kinetics and DNA damage.</p><p><strong>Materials and methods: </strong>Highly purified pUC19 plasmid DNA in aqueous oxygenated buffer was irradiated with 142.4 MeV protons at CONV (∼1.0 Gy/s) or UHDR (>455 Gy/s). Samples were positioned in a water phantom and received doses ranging from 0 to 160 Gy at LETd (dose-averaged LET) values of 1.7, 4.4, and 6.5 keV/µm. Strand breaks and formamidopyrimidine-DNA glycosylase (Fpg) and endonuclease III (Nth) -sensitive base lesions were quantified by gel electrophoresis. At low LET, fluence was estimated from Φ = ρD/LET and mean nearest-track distance from dNN = 1/(2√Φ), using the dose delivered during one OH^• lifetime (approximately 0.91 µs).</p><p><strong>Results: </strong>At LETd = 1.7 keV/µm, the estimated mean nearest-track distance during one OH^• lifetime was ∼275 µm at 1.0 Gy/s and ∼13 µm at 455 Gy/s. Even the UHDR value was ∼100 times larger than the ∼0.1-0.13 µm OH^• diffusion distance. Above ∼40 Gy cumulative dose, UHDR produced lower strand-break and enzyme-sensitive lesion values than CONV, and the CONV-UHDR difference decreased as LETd increased.</p><p><strong>Conclusions: </strong>Proton dose-rate alone is insufficient to predict the FLASH effect-mediated tissue sparing. Our results demonstrate that sparing is a track-structure-dependent kinetic phenomenon. LET, fluence, spill structure, solution chemistry, and oxygen history must be considered together. The data constrain candidate FLASH mechanisms but do not identify a unique chemical pathway.</p><p><strong>Definitions: </strong>Spur: a localized cluster of closely spaced ionizations and excitations.Branch: a lateral secondary-electron trajectory containing one or more additional spurs.Intra-track recombination: reactions among species generated within the same primary-particle track and its branches.Inter-track recombination: reactions between species generated by different primary-particle tracks. Track coincidence requires two tracks to be created close enough in both space and time for their reactive species to meet.Mean nearest-track distance: for randomly and independently positioned, approximately parallel tracks, the average center-to-center distance from one track to its closest neighboring track.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":2.4,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149240989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promising targets to overcome radioresistance in glioblastoma: current status and future perspectives.","authors":"Qamar Abuhassan, Hamzeh J Al-Ameer, Subbulakshmi Ganesan, Priya Priyadarshini Nayak, V Ramesh Kumar, Vipasha Sharma, Ashish Singh Chauhan, Mansur Aliev","doi":"10.1080/09553002.2026.2745874","DOIUrl":"https://doi.org/10.1080/09553002.2026.2745874","url":null,"abstract":"<p><strong>Purpose: </strong>GBM remains refractory to standard radiotherapy. Resistance to IR arises from complex molecular and cellular interactions and signaling pathways. Robust DDR restores lethal and sublethal DNA breaks and limits IR cytotoxicity. These responses can be potentiated by other mechanisms, such as hypoxia, enhanced antioxidant networks, and dysregulated tyrosine kinases. These factors sustain survival signaling and promote cell cycle progression after irradiation. The immune milieu is a key factor that amplifies resistance through cellular interactions. TAMs, MDSCs, and their co-inhibitory signaling pathways are key mediators of immunosuppressive stroma that exhaust anticancer immune cells, providing a way for tumor growth and metastasis. All these cellular and molecular interactions can stimulate resistance of GBM to IR. Therefore, targeting these resistance mediators in combination therapy approaches can produce stronger and more durable effects.</p><p><strong>Conclusions: </strong>This article overviews preclinical and clinical studies with a focus on promising combination modalities with radiotherapy for radiosensitizing GBM. In this review, we suggest that targeting some immunomodulators, hypoxia, DNA damage pathways, cancer stemness, altered metabolism, and redox responses can sensitize GBM to radiotherapy. In addition, we provide evidence that effective radiosensitization strategies should be guided by tumor genotype and other biomarker status.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-22"},"PeriodicalIF":2.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149229643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of folic acid supplementation on the proportion of micronucleated erythrocytes in Balb-C mice irradiated with computed tomography.","authors":"Angélica Barros-Hernández, Abel Montes-Corona, Guillermo Moisés Zúñiga-González, Roberto Francisco Garibaldi-Covarrubias, Vanessa Nabila López-Naya, Silvia Jiménez-Sánchez, Martha Patricia Gallegos-Arreola, Blanca Miriam Torres-Mendoza, Belinda Claudia Gómez-Meda","doi":"10.1080/09553002.2026.2747691","DOIUrl":"https://doi.org/10.1080/09553002.2026.2747691","url":null,"abstract":"<p><strong>Purpose: </strong>The increasing use of computed tomography (CT) has been linked to a higher risk of brain tumors and leukemia in pediatric populations. CT causes direct DNA damage and generates reactive oxygen species, leading to double-stranded DNA breaks. Folic acid, known for its role in DNA stabilization, was supplemented before radiation exposure to reduce these effects. This study assessed the genotoxic impact of CT in mice with and without folic acid supplementation.</p><p><strong>Materials and methods: </strong>Three groups of Balb-C mice were formed: Group 1 - negative control; Group 2 - exposed to total body X-rays (4 mGy per day for 2 consecutive days); and Group 3 - treated like group 2 but also given folic acid in their drinking water (Lab. Valdecasas, S.A., 2.12 mg/kg) from the start of the experiment. Blood smears were made to analyze micronucleated erythrocytes and micronucleated polychromatic erythrocytes under a microscope (100×).</p><p><strong>Results: </strong>At 72 hours, group 3 showed a reduction in micronucleated polychromatic erythrocytes compared to group 2. Both intragroup and intergroup analyses revealed a decreased proportion of micronucleated erythrocytes at 72 hours in group 3, with further reductions seen in intragroup comparisons at 96 hours.</p><p><strong>Conclusion: </strong>Folic acid reduced radiation-induced DNA damage in group 3 (CT plus folic acid) compared to group 2 (CT only), indicating a possible protective effect. However, since a murine model was used, these findings require cautious interpretation and thorough translational evaluation before validating antioxidant supplementation in pediatric patients undergoing radiation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149229710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial modulation enhances the effects of radiation on prostate cancer cells: a targeted mass spectrometry metabolomics study.","authors":"Biqi Chen, Linda J Rogers, Kelvin Liu, Sida Chen, Rexhmir Deda, Michael P Gotsbacher, Jean Yang, David R McKenzie, Natalka Suchowerska, Ann H Kwan","doi":"10.1080/09553002.2026.2739305","DOIUrl":"https://doi.org/10.1080/09553002.2026.2739305","url":null,"abstract":"<p><strong>Purpose: </strong>Spatially modulated radiation fields, such as microbeam or grid therapy, have been shown to enhance tumor cell killing while sparing normal tissue compared to uniform fields of the same total dose. The biological mechanisms underlying this effect remain poorly understood. This study investigates metabolomic changes in prostate cancer and normal prostate cells following exposure to spatially modulated versus uniform irradiation.</p><p><strong>Materials and methods: </strong>Prostate cancer (LNCaP) and normal prostate (PNT1A) cells were irradiated using spatially modulated or uniform fields at equivalent, higher, or lower mean doses. Metabolites from cellular extracts were profiled using targeted liquid chromatography-mass spectrometry (LC-MS) selected reaction monitoring (SRM). Multivariate analyses, including principal component analysis (PCA) with bootstrapping, were applied to identify dose- and field-dependent metabolic signatures.</p><p><strong>Results: </strong>PCA revealed a clear dose-response in uniformly irradiated LNCaP cells and distinct separation between non-irradiated and irradiated groups. Notably, cancer cells exposed to modulated fields clustered closer to those treated with a higher-dose uniform field than to those receiving the same mean dose, suggesting biological equivalence to dose escalation. Key metabolites driving this separation were associated with phenylalanine, tyrosine, and tryptophan biosynthesis pathways, indicating heightened metabolic stress under modulated irradiation. In contrast, PNT1A cells showed no significant metabolic distinction between modulated and uniform fields of the same mean dose.</p><p><strong>Conclusion: </strong>Spatially modulated irradiation induces metabolic responses in prostate cancer cells comparable to those elicited by higher-dose uniform fields, while normal cells respond similarly to uniform fields of the same dose. These findings provide mechanistic insight into the therapeutic advantage of spatial modulation and support its potential role in improving tumor control without increasing normal tissue toxicity. Given that clinical radiotherapy sessions typically span 15-30 minutes, incorporating spatial modulation and temporal fractionation strategies into treatment workflows may offer a practical and effective means to improve therapeutic outcomes in prostate cancer.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149229671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CDK4/6 inhibition radiosensitizes p16-negative squamous cell carcinomas of the aerodigestive tract via cell cycle arrest.","authors":"Meghan Lambie, Aakshi Puri, Rita Gill, Ariana Rostami, Maria Kondratyev, Bradly G Wouters, Laurie Ailles, Benjamin Haibe-Kains, Scott V Bratman","doi":"10.1080/09553002.2026.2739263","DOIUrl":"https://doi.org/10.1080/09553002.2026.2739263","url":null,"abstract":"<p><strong>Introduction: </strong>CDK4/6 inhibitors have quickly entered clinical guidelines for luminal breast cancer, but their role in other cancer types remains to be defined. Squamous cell carcinomas not driven by human papillomavirus (HPV-negative SCCs) are frequently treated with radiotherapy, and concurrent treatment with CDK4/6 inhibitors has been proposed. We sought to investigate the efficacy of this combination across a range of HPV-negative SCCs and define molecular biomarkers of response that could be used for treatment selection.</p><p><strong>Materials and methods: </strong>We employed clonogenic assays and a previously described long-term viability assay for measuring radiation response to identify radioresistant cells within HPV-negative SCC cell lines. Among consistently radioresistant lines, we conducted drug/radiation combinations to measure additive effects of the CDK4/6 inhibitors, abemaciclib and palbociclib. Mechanistic effects of drug/radiation combinations were further investigated by flow cytometry, immunocytochemistry, and western blot. Efficacy was validated in vivo using an HPV-negative SCC cell line xenograft.</p><p><strong>Results: </strong>Among the 19 HPV-negative SCC cell lines, 6 were consistently radioresistant using both clonogenic and viability assays. We validated the use of the viability assay for drug/radiation combinations using known radiosensitizing drug, cisplatin, before applying it to the CDK4/6 inhibitors. Five of the 6 radioresistant cell lines displayed a favorable combination effect with both CDK4/6 inhibitors. Sensitivity to the combination was reliant on drug-induced cell cycle arrest and correlated with lack of p16 activity in the presence of Rb. Combining abemaciclib with radiation reduced tumor growth in xenografted mice.</p><p><strong>Conclusions: </strong>CDK4/6 inhibitors are good candidates for further preclinical and clinical evaluation in combination with radiation in HPV-negative SCCs due to their effects on cell cycle phase in cells with Rb protein activity and low p16 levels. The long-term viability assay allows for efficient testing of drug/radiation combinations and can be considered for future experiments as part of a bench-to-bedside translational pipeline.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":2.4,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149198705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vortex beam-based Raman spectroscopic single cell analysis of radiation-induced biochemical changes in human erythrocytes for radiation biodosimetry.","authors":"Solanki Shil, Panchanil Sarmah, Aseefhali Bankapur, Krishna Kishore Mahato, Kamalesh Dattaram Mumbrekar","doi":"10.1080/09553002.2026.2713037","DOIUrl":"https://doi.org/10.1080/09553002.2026.2713037","url":null,"abstract":"<p><strong>Introduction: </strong>Radiation biodosimetry plays a vital role in assessing radiation exposure levels and determining potential health risks for individuals affected by radiological incidents or accidental exposures. Accurate and rapid classification of radiation-exposed samples is essential for effective triage, medical treatment, and public health management. This study investigates the potential of Raman Tweezer Spectroscopy (RTS) as a label-free, single-cell spectroscopic approach for detecting and discriminatating radiation-induced biochemical alterations in human red blood cells (RBCs).</p><p><strong>Methods: </strong>Human RBCs were exposed to X-ray doses ranging from 0.5 to 8 Gy and analyzed using Raman tweezers with 785 nm excitation. The resulting vibrational spectra captured molecular signatures arising primarily from hemoglobin, membrane lipids, and protein backbones. Multivariate statistical analysis was applied to the spectral data to identify dose-dependent biochemical variance and to evaluate the feasibility of discriminating between irradiated and non-irradiated samples at the single-cell level.</p><p><strong>Results: </strong>Principal component analysis (PCA) revealed progressive spectral divergence with increasing radiation dose, with distinct response regimes observed at low (0.5 Gy), moderate (2-4 Gy), and high (6-8 Gy) doses. Loading analysis indicated that radiation-induced changes were dominated by lipid- and protein-associated Raman bands, consistent with oxidative modification of membrane and protein structures. Increased intercellular heterogeneity was observed at intermediate-to-high doses.</p><p><strong>Discussion: </strong>These results demonstrate that RTS is sensitive to radiation-induced biochemical alterations in individual RBCs and can differentiate between exposure levels without labels or extensive sample preparation. The findings support the feasibility of this approach as a complementary tool for rapid radiation biodosimetry, with potential applications in emergency response, medical triage, and radiobiological event management. As an unsupervised, proof-of-concept analysis, the present work establishes the detection and discrimination of radiation-induced biochemical change rather than a validated quantitative dose-reconstruction methodology.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cohort methods: the CANadian RADiation workers (CANRAD) cohort.","authors":"Rachel S D Lane, Tim Prendergast, Laura A Rodriguez-Villamizar, Phillippe Prince, Lydia B Zablotska","doi":"10.1080/09553002.2026.2733660","DOIUrl":"10.1080/09553002.2026.2733660","url":null,"abstract":"<p><strong>Purpose: </strong>The long-term follow-up of workers with protracted exposure to low dose occupational ionizing radiation is important to understand the health effects and risks of radiation. This paper describes the creation and record linkage of the CANadian RADiation workers (CANRAD) cohort, including the work to resolve data issues and linkage challenges to outcome databases.</p><p><strong>Materials and methods: </strong>The CANRAD cohort includes all workers with occupational exposure to ionizing radiation (1951-2018) in Canada's National Dose Registry (NDR) and three facility-based sub-cohorts: Eldorado uranium workers (1932-1980), Ontario uranium miners (1954-1996), and Atomic Energy of Canada Limited nuclear workers (1956-1985). The sub-cohorts were added to the NDR to update workers' employment histories and radiation exposures to 2018. Deterministic and probabilistic record linkage methods linked the CANRAD cohort to national population-based mortality (1950-2020), cancer incidence (1969-2019), and tax (1981-2020) data.</p><p><strong>Results: </strong>Of 957,580 CANRAD workers, 883,150 (92.2%) successfully linked to Statistics Canada vital statistics databases. The analytical cohort includes 860,650 (89.9%) workers, including 427,920 (49.7%) females. At the end of follow-up, 751,520 (87.3%) of the cohort were alive and 94,410 (11.0%) were dead. Neoplasms and circulatory disease represented 63.8% (57,030) of all known causes of death (89,330). Prostate, breast, lung & bronchus, and colorectal cancer represented 49.9% (40,780) of first primary cancers (81,670). The mean cumulative effective dose was 17.1 mSv; but most cumulative doses were <10 mSv (∼85.0% of males and 98.1% of females). Females (350,510, 82.4%) dominated the medical sector.</p><p><strong>Conclusions: </strong>The CANRAD cohort is a large and valuable cohort of Canadian occupationally exposed radiation workers with employment histories, radiation exposures and long-term follow-up of tax, mortality, and cancer incidence. Analysis of this cohort will support the radiation protection framework.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a small ('silent') rate beta-irradiation field on the response of human neutrophil outer zeta potential to adrenergic and adenosinergic ligands.","authors":"Victor Zhirnov, Igor Iakovenko, Volodymyr Brovarets","doi":"10.1080/09553002.2026.2682846","DOIUrl":"https://doi.org/10.1080/09553002.2026.2682846","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to investigate the zeta potential (ZP) response of human neutrophils to well-characterized ligands of adrenergic and adenosine receptors under a small rate β-radiation (SRβR) field.</p><p><strong>Materials and methods: </strong>The cells were exposed to ionizing radiation by adding ¹⁴C-leucine to the cell incubation medium at dose rates ranging from 0.01 to 100 <i>μ</i>Gy·h^-1 at 10-fold intervals over 1 h. The ZP was calculated from the electrophoretic mobility value using Zeta for Windows.</p><p><strong>Results and conclusions: </strong>As the dose rate increases, the surface negative charge of neutrophils also increases, and the curve passes through a maximum at 10 μGy·h^-1. It was shown that the SRβR increases neutrophil ZP due to the dose rate, rather than the absorbed dose, and depends on the interaction of the tested ligands with the plasma membrane as a whole, and not only with the targets of these ligands. Most ligands induced unidirectional changes in ZP within the applied concentrations, the direction of which was ligand-dependent. Only yohimbine showed concentration-dependent, oppositely directed ZP changes. Most ZP responses to ligands were modified by the radiation. The data obtained indicate that alteration of the response of the outer layer of the plasma membrane of human neutrophils to receptor ligands under the influence of a small rate radiation field can alter the ZP-dependent immune functions of neutrophils, at least in vitro.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}