International journal of radiation biology最新文献

{"title":"Exploring Auger electron-emitting radionuclides for targeted DNA damage in mismatch repair-deficient cells: a theoretical study of ⁹⁹Rh- and ¹²³I-labeled metalloinsertors.","authors":"Alexandre França Velo, Lukas Carter, Michael Bellamy, Mike A Cornejo, Brian M Zeglis, John L Humm","doi":"10.1080/09553002.2025.2517328","DOIUrl":"https://doi.org/10.1080/09553002.2025.2517328","url":null,"abstract":"<p><strong>Purpose: </strong>Preserving the integrity of the genome is critical to healthy cellular growth and development. Under normal circumstances, the eukaryotic mismatch repair (MMR) machinery is effective at detecting DNA polymerase errors and maintaining the fidelity of the genome. However, cells with inactivated MMR machinery are prone to the accumulation of mutations and tumorigenesis. This study explores the theoretical potential of rhodium-99- and iodine-123-labeled DNA metalloinsertors as Auger electron-emitting radiotherapeutics for cancers characterized by MMR deficiency.</p><p><strong>Materials and methods: </strong>A Monte Carlo code was developed in MATLAB^® to obtain Auger electron energy spectra for ⁹⁹Rh and ¹²³I. Using Geant4 track structure simulations, we determined the difference in effectiveness of these two Auger electron-emitting radionuclides in direct damage to DNA and the ability to produce double strand break damage (dsb) to the DNA comparing two different constructors 'G4EmDNAPhysics_option2' and 'G4EmDNAPhysics_option4'.</p><p><strong>Results: </strong>Differences in the Auger electron emission spectra of ⁹⁹Rh and ¹²³I arise from their electronic structure: ¹²³I favors more complex cascades and ultra-low-energy electrons, while ⁹⁹Rh produces electrons with energies more suited to DNA damage. Despite similar total electron yields, the emissions of ⁹⁹Rh are more effective at causing dsb (0.71 <i>vs.</i> 0.60 dsb/decay for ⁹⁹Rh and ¹²³I, respectively, using constructor 'G4EmDNAPhysics_option2' and 0.81 dsb/decay for ⁹⁹Rh <i>vs.</i> 0.71 dsb/decay for ¹²³I when using 'G4EmDNAPhysics_option4'.</p><p><strong>Conclusion: </strong>This theoretical study leverages both simulation and comparative analyses to identify ⁹⁹Rh as a promising Auger electron-emitting nuclide for radiotheranostics, as it offers superior DNA damage efficacy compared to ¹²³I.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiobiological mechanisms of radium-223 dichloride: a nonclinical study.","authors":"Jin Young Baik, Yiseul Choi, Eun Sang Lee, Hye Kyung Chung","doi":"10.1080/09553002.2025.2519516","DOIUrl":"https://doi.org/10.1080/09553002.2025.2519516","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to elucidate the radiobiological mechanisms of radium-223 dichloride ([²²³Ra]RaCl₂)-an FDA-approved agent for metastatic castration-resistant prostate cancer-by investigating its in vitro and in vivo toxic effects.</p><p><strong>Method: </strong>Using both the CCK8 and clonogenic assays, our in vitro experiments evaluated the inhibition of cell proliferation in response to variations in applied activity and exposure duration in human cancer cell lines (MDA-MB-231, BxPC3, A549, and PC3) as well as in rat pancreatic cancer cells (AR42J). Molecular analyses utilizing SDS-PAGE characterized the activation of specific cell death pathways. Additionally, an in vivo investigation employing intravenous administration further examined the systemic toxicity of [²²³Ra]RaCl₂ by monitoring animal mortality, body weight, and hematological parameters following treatment with varying activity levels (74, 148, and 222 kBq/head).</p><p><strong>Results: </strong>[²²³Ra]RaCl₂ significantly inhibited cell proliferation in an applied activity- and time-dependent manner in human cancer cell lines, whereas AR42J cells showed relative resistance. Molecular findings revealed that BxPC3 cells underwent apoptosis via caspase-3 cleavage; PC3 cells experienced pyroptosis marked by gasdermin E (GSDME) activation and caspase-7 cleavage; and MDA-MB-231 cells exhibited enhanced PARP-1 activation indicative of DNA double-strand breaks. In vivo, treated animals demonstrated significant differences in mortality, body weight, and hematological parameters compared to controls, mirroring myelosuppressive effects typical of radiotherapy.</p><p><strong>Conclusion: </strong>The findings provide critical insights into the differential cytotoxic mechanisms of [²²³Ra]RaCl₂ across various cancer cell types and highlight its systemic toxicity in vivo. This improved understanding may facilitate the development of safer and more effective therapeutic radiopharmaceuticals based on [²²³Ra]RaCl₂.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remembering Dr. Ronald E.J. Mitchel.","authors":"Edouard I Azzam, Candice Didychuk, Douglas R Boreham, Marilyne Stuart","doi":"10.1080/09553002.2025.2516478","DOIUrl":"https://doi.org/10.1080/09553002.2025.2516478","url":null,"abstract":"","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144277142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an electron spin resonance spectroscopy code for measuring carbonate radicals in tooth enamel and verification of its practicality using irradiated Japanese macaque teeth.","authors":"Takuma Yamashita, Teppei Hayashi, Yusuke Mitsuyasu, Kenta Ono, Satone Iwami, Yasushi Kino, Tsutomu Sekine, Toshitaka Oka, Atsushi Takahashi, Yoshinaka Shimizu, Mirei Chiba, Toshihiko Suzuki, Ken Osaka, Keiichi Sasaki, Taku Sato, Rio Isobe, Masatoshi Suzuki, Tomisato Miura, Manabu Fukumoto, Hisashi Shinoda","doi":"10.1080/09553002.2025.2510676","DOIUrl":"https://doi.org/10.1080/09553002.2025.2510676","url":null,"abstract":"<p><strong>Purpose: </strong>We developed a new computer program for the application of electron spin resonance (ESR) to dosimetry of wild animals related to the Fukushima Daiichi Nuclear Power Plant accident.</p><p><strong>Materials & methods: </strong>The ESR spectra of carbonate radicals and other inorganic radicals are calculated by the complete elliptic integral. A simulated annealing method is implemented for the parameter optimization. A cost function is designed to include the second derivative form of the microwave absorption spectrum to improve the fitting accuracy. As a testing ground for the developed code, we prepared tooth enamel samples from a Japanese macaque captured in a control area.</p><p><strong>Results: </strong>The developed code well reproduced the measured ESR spectrum. With a test spectrum, we demonstrated that the cost function that includes the second derivative form of the microwave absorption spectrum is helpful for the precise analysis of the low-dose enamel samples. The smoothness of the ESR spectrum plays an important role in utilizing this feature.</p><p><strong>Conclusion: </strong>The developed computer code can be used to analyze the ESR spectrum of tooth enamels of Japanese macaques. A precise analysis is essential to lower the detection limit and expand the applicability of ESR dosimetry. The code is independent of the computer operating system and is available publicly.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective centrifugation techniques for improving the frequency of metaphase spreads in lymphocytopenic patients.","authors":"Hyo Jin Yoon, Hae Young Ko, Su San Yang, Soon Woo Kwon, Minsu Cho, Jin Kyung Lee, Tomisato Miura, Yohei Fujishima, Donovan Anderson, Yang Hee Lee","doi":"10.1080/09553002.2025.2510674","DOIUrl":"https://doi.org/10.1080/09553002.2025.2510674","url":null,"abstract":"<p><strong>Purpose: </strong>Cytogenetic biodosimetry is used for radiation dose assessment by evaluating chromosomal aberrations in peripheral blood lymphocytes. However, high-dose radiation may cause low absolute lymphocyte counts (ALCs), making it difficult to obtain sufficient metaphase spreads for analysis. This study aimed to optimize centrifugation settings to enrich metaphase spreads, particularly for lymphocytopenic patients.</p><p><strong>Materials and methods: </strong>Peripheral blood samples from four healthy donors and one lymphocytopenic patient were collected. Lymphocytes were harvested using one of four centrifugation settings. After an additional low-speed centrifugation step (200 × <i>g</i> for 1 minute) in each experiment, we evaluated slide quality using mitotic index (MI) and metaphase frequency (MF), which were calculated by the number of metaphases and blasts using Metafer 4 software.</p><p><strong>Results: </strong>We established an automatic measurement method for metaphases and blasts, adjusting the settings of Metafer 4. In four healthy donors, the strongest centrifugation (Exp. 4, 879 × <i>g</i> for 5 minutes) yielded the highest number of 'all cells' after harvest. Incorporating an additional low-speed centrifugation step significantly increased MF by 1.5- to 2-fold across all settings, with the greatest improvement observed in Exp. 4. This approach was applied to a lymphocytopenic patient, resulting in a 3.5-fold improvement of MF and the production of high-quality slides. MI was not significantly affected by centrifugation.</p><p><strong>Conclusions: </strong>The combined high-speed and additional low-speed centrifugation method increased MF, improved slide quality by eliminating lower-density cells, and made it easier to analyze metaphase spreads. This method could be used for obtaining sufficient metaphase spreads in lymphocytopenic patients.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of adipocyte-derived stem cells treatment for moderate radiodermatitis: animal model.","authors":"Cristina Pires Camargo, Juan Pablo Menendez Zavala, Gabriela Park Serafim, Heloisa Andrade Carvalho, Bianca Loula Santos, Viviane Abreu Nunes, Miyuki Uno, Maria José Ferreira Alves, Tatiane Katsue Furuya, Rolf Gemperli","doi":"10.1080/09553002.2025.2505534","DOIUrl":"https://doi.org/10.1080/09553002.2025.2505534","url":null,"abstract":"<p><strong>Purpose: </strong>Cancer treatment frequently involves the use of radiotherapy, which is associated with several adverse effects, including fatigue, immunosuppression, and radiodermatitis. Radiodermatitis affects up to 95% of patients worldwide. This study aimed to evaluate the therapeutic potential of adipose-derived stem cells (ASCs) in treating radiodermatitis in a rat model.</p><p><strong>Materials and methods: </strong>Inguinal fat of three rats was removed, and ASC isolated. Then, 20 male Wistar rats underwent radiodermatitis induction using two Strontium-90 plaques, each delivering a single dose of 18 Gy to dorsal areas. After 15 days, animals were divided into two groups. (1) Control group (<i>n</i> = 10): without treatment; (2) ASC group (<i>n</i> = 10): treated with subcutaneous 1 × 10⁶ ASC injections into radiodermatitis sites. After 15 more days, skin was evaluated with RTOG scale, and tissue samples were collected to perform histology and gene expression of inflammatory markers.</p><p><strong>Results: </strong>ASC group showed a lower degree of radiodermatitis according to the RTOG scale. ASC showed an increased number of arterioles (<i>p</i> = .007) and a reduction in number of inflammatory cells (<i>p</i> = .02) compared to control. Furthermore, the treatment led to approximately a 50% reduction in the gene expression levels of the inflammation markers <i>Il1rap</i> (<i>p</i> < .001), <i>Mmp3</i> (<i>p</i> = .001), and <i>Tnf</i> (<i>p</i> = .004) as well as a 30% reduction in <i>Cd68</i> (<i>p</i> = .026) expression in ASC when compared to control.</p><p><strong>Conclusions: </strong>This study suggests that the application of ASC enhances wound healing process in radiodermatitis by mitigating inflammation. This approach shows potential to improve outcomes and quality of life for patients undergoing radiotherapy.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High HPV viral load predicts worse prognosis in patients with cervical cancer treated with radiochemotherapy.","authors":"Ying Wang, Depu Wang, Haiyan Chen, Yingbing Zhang, Meng Cao, Zhaoji Lan, Yang Zhang, Yili Wang, Hongwei Chen","doi":"10.1080/09553002.2025.2510668","DOIUrl":"https://doi.org/10.1080/09553002.2025.2510668","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the prognostic value of high-risk human papillomavirus (HR-HPV) viral load in long-term survival of cervical cancer (CC) patients.</p><p><strong>Methods: </strong>Two hundred and twenty-nine CC patients from 2009 to 2013 who underwent radiochemotherapy (RCT) or plus hyperthermia (HT) were recruited. HPV type 16, 18, 58 DNA was detected by in situ hybridization (ISH) and either HR-HPV type with the highest intensive signals was chosen as the evaluating target. HPV viral load was evaluated by semiquantitative analysis for the intensity of ISH signal captured from the colonial cancer nests with the highest density of signal on section from each case. RNA scope was used to detect the HPV E6/E7 mRNA and P53/Rb mRNA expression. Immunohistochemistry (IHC) was used to detect P53/Rb protein expression.</p><p><strong>Results: </strong>The number of patients with low and high HPV viral load was 152 (66.38%) and 77 (33.62%), respectively. 5-year Overall Survival (OS) of patients in the low and high HPV DNA group were 82.9 and 63.6%, respectively (<i>p</i> = .001). 5-year Local Relapse-Free Survival (LRFS) of patients in the low and high HPV DNA group were 80.3 and 62.3%, respectively (<i>p</i> = .004). Univariate and multivariate analyses also confirmed the higher risk death of high virus load group. HR-HPV oncogene mRNA expression was highly concert with HPV DNA. Radiation enhanced HPV E6/E7 mRNA and P53/Rb mRNA expressions and decreased P53/Rb protein expressions in tissue samples and cancer cell lines.</p><p><strong>Conclusion: </strong>High initial viral load measured in cervical tumor nest was a strong independent prognostic factor that influenced poor survival in CC after RCT.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144210559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformation of the Chornobyl NPP cooling pond: radioecological situation and its impact on the blood system of small rodents.","authors":"Natalia Riabchenko, Alla Lypska, Natalia Rodionova, Hiroko Ishiniwa, Kenji Nanba, Olena Burdo","doi":"10.1080/09553002.2025.2505529","DOIUrl":"https://doi.org/10.1080/09553002.2025.2505529","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the radioecological consequences of the technogenic transformation of the Chornobyl NPP cooling pond aquatic ecosystem and its impact on the blood system of <i>Myodes glareolus</i>, a typical small rodent species in the Chornobyl exclusion zone.</p><p><strong>Materials and methods: </strong>Animals were captured in the drained areas of the cooling pond and nearby areas. Radiometric surveys of the animals' natural habitat and soil sampling were conducted. γ-β-spectrometry was used to measure radionuclide levels (¹³⁷Cs and ⁹⁰Sr) in soil and animal bodies. Absorbed dose rates were calculated. Blood smears, bone marrow and spleen imprints were prepared and stained using the standard Pappenheim method.</p><p><strong>Results: </strong>Activity of ¹³⁷Cs and ⁹⁰Sr in soil and animal samples, external/internal doses in animals were estimated. Radioecological assessment showed that radionuclide contamination in the drained areas did not exceed that of the adjacent territory. In exposed animals, similar hematopoietic alterations were observed, including disrupted differentiation and maturation of bone marrow cells, particularly in erythroid and granulocytic lineages. However, these blood abnormalities were less pronounced in animals from the drained zones.</p><p><strong>Conclusions: </strong>Draining the cooling pond, despite the expected high radionuclide concentrations in bottom sediments, did not cause significant changes in surface soil contamination. While bank voles showed deviations in blood parameters compared to reference values, differences between exposure groups were generally not significant. These findings support continued monitoring of radiobiological effects associated with the transforming of radiation-contaminated aquatic ecosystems into terrestrial.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imidazolyl ethanamide pentandioic acid promotes hematopoietic recovery following sublethal radiation in a murine model.","authors":"Suresh Veeraperumal, Basveshwar Gawali, Nikita Singh, Seren Sevim-Wunderlich, Maciej T Czajkowski, Srishti Munjal Mehta, Corinna Asang, Dirk Pleimes, Snehalata A Pawar","doi":"10.1080/09553002.2025.2505526","DOIUrl":"https://doi.org/10.1080/09553002.2025.2505526","url":null,"abstract":"<p><strong>Purpose: </strong>Hematopoietic acute radiation syndrome (H-ARS) results from bone marrow (BM) damage, leading to depletion of myeloid and lymphoid cells and increasing infection, hemorrhage, and mortality risks. Imidazolyl ethanamide pentandioic acid (IEPA), an orally bioavailable molecule, alleviates chemotherapy-induced myelosuppression and may similarly mitigate H-ARS. This study examines whether IEPA, alone or with granulocyte-monocyte colony-stimulating factor (GM-CSF), promotes recovery of hematopoietic stem and progenitor cells (HSCs/HPCs) after sublethal total body irradiation (TBI).</p><p><strong>Materials and methods: </strong>Mice received 5 Gy TBI followed by vehicle, IEPA (days 1-3), GM-CSF (days 1-5), or IEPA+GM-CSF. Recovery was assessed by body weight, blood counts, flow cytometry, clonogenic assays, BM megakaryocytes, and splenic CD34⁺ cells.</p><p><strong>Results: </strong>IEPA increased body weight 2.5-fold by day 7, while IEPA+GM-CSF showed 2.5-fold and 3.3-fold increases on days 7 and 21, respectively. IEPA promoted recovery of HSCs/HPCs, multipotent progenitors (MPP2), myeloid and lymphoid progenitors, BM megakaryocytes, and splenic CD34+ cells. IEPA+GM-CSF improved in vitro self-renewal but offered no major in vivo advantage over IEPA alone, except for a 159-fold recovery in erythroid progenitors. All treatments reduced γ-H2AX expression in Lin- and HPCs on day 21, indicating less DNA damage.</p><p><strong>Conclusions: </strong>Our results reveal that IEPA attenuated radiation-induced DNA damage and improved recovery of HSCs/HPCs, myeloid as well as lymphoid progenitors, accompanied by increases in platelets in blood, megakaryocytes in BM and splenic CD34⁺ cells. IEPA shows promise as a radiation mitigator, with no significant benefit observed from adding GM-CSF.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative splicing in human cells exposed to ionizing radiation: a comprehensive review of ex vivo and in vivo studies.","authors":"Jerónimo Leberle, Marina Perona, Ananya Choudhury, Nicolas Bellora, Irene L Ibañez, Luisa V Biolatti","doi":"10.1080/09553002.2025.2505524","DOIUrl":"https://doi.org/10.1080/09553002.2025.2505524","url":null,"abstract":"<p><strong>Purpose: </strong>This study reviews how ionizing radiation (IR) induces alternative splicing (AS) in non-tumor and tumor cells under both ex vivo and in vivo irradiation conditions. The relevance and limitations of IR-induced AS in identifying potential biomarkers are highlighted for two main applications: biodosimetry and radiotherapy.</p><p><strong>Conclusions: </strong>Radiation promotes alterations in AS, which may differentially affect the response in both tumor and non-tumor cells. This response can occur in genes that change their overall expression as well as in those that remain unaltered in response to IR. Although cis-regulators modulate AS, trans-regulators like splicing factors are more involved in the IR response. Variants of key genes involved in the DNA damage response (DDR) are regulated in non-tumor cells while they are often deregulated in tumor cells favoring radioresistance. Identifying IR-induced AS variants could enhance the sensitivity of biodosimeters for dose estimation and biomarkers for radiosensitivity, offering potential strategies to personalize radiotherapy and improve outcomes. New and advanced sequencing technologies will allow variant identification important for the field of radiobiological research.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}