International journal of radiation biology最新文献

{"title":"Dose-response of X-ray induced dicentrics determined by fully- & semi-automated DCScore evaluation after 3 h or 24 h colcemid treatment.","authors":"Anna-Lea Graf, Matthias Port, Christina Beinke","doi":"10.1080/09553002.2025.2523283","DOIUrl":"https://doi.org/10.1080/09553002.2025.2523283","url":null,"abstract":"<p><strong>Purpose: </strong>Optimization of automated dicentric evaluation in the BIR laboratory is necessary to improve and accelerate individual biological dosimetry in radiation accident scenarios. Therefore, two different DCScore classifiers were analyzed for their suitability for use with laboratory-specific protocols, including two different lymphocyte culture conditions, 3-hour or 24-hour colcemid treatment.</p><p><strong>Materials and methods: </strong>Dicentric formation was compared in 3 h and 24 h colcemid-treated cultures by fully- and semi-automated dicentric scoring using two different classifiers. Various calibration curves were constructed and absorbed doses of blinded X-irradiated blood samples were estimated after 24 h of colcemid treatment using both classifiers and scoring modes.</p><p><strong>Results: </strong>24 h colcemid treatment results in twice as many metaphases as 3 h colcemid treatment and the courses of dicentric frequencies after short- and long-term colcemid treatment differ, especially > 1 Gy. The \"short-term colcemid classifier\" detects more dicentric candidates and true positive dicentrics, respectively, especially > 2 Gy than the \"long-term classifier\" on the same slides.</p><p><strong>Conclusion: </strong>Neither classifier was significantly better suited for the lab-specific MP preparations with regard to triage dose estimates for blinded samples by fully- as well as semi-automated analysis. For accurate dose assessment, it is recommended to adapt an available classifier to laboratory-specific conditions and protocols to optimize the identification of true dicentrics by DCScore.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tmprss6</i> modulates radiation-induced liver injury through the hepcidin axis and PI3K/AKT pathway.","authors":"Ya-Li Zhou, Zhen-Xin Wang, Jia-Qin Gao, Jie Xu, Lei Qin, Yang He, Ming Li, Jun He","doi":"10.1080/09553002.2025.2527894","DOIUrl":"https://doi.org/10.1080/09553002.2025.2527894","url":null,"abstract":"<p><strong>Background: </strong>Radiation-induced liver injury (RILI) poses a significant challenge in abdomino-pelvic tumor radiotherapy, adversely impacting normal liver tissues. This study explores the role of <i>Tmprss6</i>, a gene encoding Matriptase2, in acute RILI development and its impact on hepatocyte apoptosis.</p><p><strong>Methods: </strong>A RILI model was established using 30 Gy total liver irradiation in C57BL/6J mice. Expression of <i>Tmprss6</i> and hepcidin post radiation was detected by immunohistochemistry staining, Western blot and quantitative real-time PCR. <i>Tmprss6</i> knockout mice were established to demonstrate the contribution of <i>Tmprss6</i> to RILI. The molecular response within the liver after <i>Tmprss6</i> knockdown was investigated using Transcriptomics sequencing.</p><p><strong>Results: </strong>Disruptions in the Matriptase2-hepcidin axis emerge post-total liver radiation, marked by decreased Matriptase2 levels and heightened hepcidin levels. <i>Tmprss6</i> knockout exacerbates RILI, evident in reduced mouse survival, increased liver damage, and elevated serum levels of aminotransferases. Additionally, <i>Tmprss6</i> deletion increases inflammatory cytokines and malondialdehyde level while diminishing liver antioxidant capacity. Gene expression profiling reveals shifts in inflammation, apoptosis, and p53 signaling pathways upon <i>Tmprss6</i> deletion. In vitro experiments, utilizing the AKT inhibitor AZD5363, demonstrate its effectiveness in reversing impediments caused by <i>Tmprss6</i> silencing post-radiation. AZD5363 effectively restores suppressed cell proliferation, mitigates heightened cell apoptosis, and counters the elevated p53 expression induced by <i>Tmprss6</i> depletion. This underscores the partial mediation of <i>Tmprss6</i>'s protective role through the PI3K-AKT signaling.</p><p><strong>Conclusions: </strong>This study unveils intricate mechanistic pathways activated by <i>Tmprss6</i> silencing, amplifying p53 expression, facilitating hepatocyte apoptosis, and accelerating RILI progression, which providing nuanced insights into the multifaceted involvement of <i>Tmprss6</i> in RILI.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of DNA-protein crosslink levels in HEK293T cells in first minutes after irradiation using comet assay.","authors":"Anna Rybak, Elena Belykh, Aleksei Chernykh, Ilya Velegzhaninov","doi":"10.1080/09553002.2025.2527898","DOIUrl":"https://doi.org/10.1080/09553002.2025.2527898","url":null,"abstract":"<p><strong>Background: </strong>The role of DNA-protein crosslinks (DPCs) in radiation-induced damage effects in human cells and the dynamics of their repair following sublethal irradiation remain poorly understood. Unrepaired DPCs can lead to genome instability. The knowledge of biological impacts of ionizing radiation-induced DPCs (IR-induced DPCs) is essential for development of cancer treatments and approaches to radioresistance regulation.</p><p><strong>Purpose: </strong>Purpose of the study was to estimate the effects of DPCs formation on radiation-induced DNA damage levels and DNA repair rate detected by comet assay in HEK293T cells.</p><p><strong>Materials and methods: </strong>HEK293T cells were gamma-irradiated at doses 2, 4, 6, and 12 Gy. The alkaline comet assay was performed; distributions of cells by % DNA in comet tail were analyzed. Cells were fixed 1, 3, 5, 10, and 30 min after irradiation to estimate DNA repair dynamic. The proteinase K treatment and irradiation at 4 °C and 37° was used to estimate the contribution of DPCs formation and repair to the distribution of cells by DNA mobility.</p><p><strong>Results and conclusions: </strong>Increased proportion of nucleoids with low electrophoretic mobility was shown in the first minutes after the γ-irradiation at 2 Gy. Proteinase K treatment of samples eliminated this effect, supporting our hypothesis of the DPCs being the main cause of this phenomenon. DPCs continued forming for several minutes after irradiation (increase of low-mobility nucleoids was observed up to 10 min after the irradiation); their repair rate was quite high (repaired within the first 30 min), comparable to the repair rate of single strand DNA breaks.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> impact of the radiation-induced bystander effect mediated by mesenchymal stem cells on leukemic cell migration: implications for the CXCL12/CXCR4 axis.","authors":"Beatriz Almeida, Rosana Lopes Rodrigues Amon, Amanda Nogueira-Pedro, Edson Naoto Makiyama, Ricardo Ambrósio Fock","doi":"10.1080/09553002.2025.2527148","DOIUrl":"https://doi.org/10.1080/09553002.2025.2527148","url":null,"abstract":"<p><strong>Purpose: </strong>The hematopoietic microenvironment, particularly mesenchymal stem cells (MSCs), plays a crucial role in hematopoiesis and cell migration. MSCs influence hematopoietic cells through their secretome, impacting cell trafficking and homing. Leukemia disrupts this environment, and while radiotherapy targets malignant cells, it may also affect surrounding cells via the radiation-induced bystander effect (RIBE). This study investigated both the direct effects of radiation on MSCs and their bystander influence on leukemic cell migration.</p><p><strong>Materials and methods: </strong>Mouse MSCs (C3H10T1/2) and leukemic cells (C1498) were cultured to assess radiation-induced apoptosis, DNA damage, and bystander effects. Conditioned media from irradiated MSCs were applied to C1498 cells to evaluate apoptosis, gene expression, adhesion, and migration using flow cytometry and RT-PCR.</p><p><strong>Results: </strong>MSCs were radiation-resistant up to 4 Gy but sustained damage at 6 Gy. Irradiated MSCs secreted elevated levels of IL-1β, sICAM-1, and CXCL-12. While the bystander effect on leukemic cells was modulated by irradiated MSCs, it did not affect survival or genes related to cell migration. However, an increase in leukemic cell migration rate mediated by the CXCL-12/CXCR4 axis was noted. Inhibition of CXCR4 with AMD3100 reduced this migration, highlighting the potential of targeting this axis for therapeutic strategies.</p><p><strong>Conclusion: </strong>The MSC-mediated bystander effect, primarily involving the CXCL-12/CXCR4 axis, appears to promote increased leukemic cell migration in vitro. While these findings provide preliminary insights into how radiotherapy may influence the hematopoietic microenvironment, further in vivo studies and validation across additional cell lines are necessary to confirm these effects and explore their potential therapeutic implications.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of stability of radiation-induced EPR signals in sorbitol.","authors":"Hasan Tuner, François Trompier, Alexander Romanyukha","doi":"10.1080/09553002.2025.2527153","DOIUrl":"https://doi.org/10.1080/09553002.2025.2527153","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study is to investigate the stability of radiation-induced electron paramagnetic resonance (EPR) signals in sorbitol and to determine the spectroscopic characteristics of the radiation-induced radicals in sorbitol.</p><p><strong>Materials and methods: </strong>Sorbitol samples were irradiated at 10 Gy using a 6 MV X-ray beam of medical linear accelerator (LINAC). EPR measurements were carried out using X-band (Bruker ESR5000X, and EMX-131) and Q-band (Bruker EMXplus) spectrometers. Isochronal and isothermal annealing experiments, as well as fading experiments, were carried out to assess the stability of radiation-induced signals. EasySpin simulation software was used to determine the spectroscopic and structural parameters of the radiation-induced radicals.</p><p><strong>Results: </strong>The EPR spectrum of irradiated sorbitol consists of several overlapping components produced by stable and unstable radicals. X- and Q-band measurements revealed significant changes in the signal patterns during time fading and thermal annealing experiments. High-temperature annealing caused rapid decay of the unstable radicals, leaving behind a stable radical. Simulation calculations indicated that at least three components were required to reproduce the observed EPR spectra. Spectroscopic parameters derived from simulations showed consistent agreement across the different experimental conditions.</p><p><strong>Conclusion: </strong>Sorbitol shows promising characteristics as an EPR dosimeter, with radiation-induced radicals exhibiting distinct thermal and time stability. High-temperature annealing can eliminate unstable radicals, enabling reliable dosimetric application shortly after irradiation. The identified stable radical is a promising marker for dose quantification. These findings support the feasibility of using sorbitol for retrospective and accidental dosimetry.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective radiation-enhancing effects of a muscle-derived dipeptide in an orthotopic non-small cell lung cancer (NSCLC) xenograft mouse model.","authors":"Li Ming Wang, Monica Serban, Osvaldo Arias, Jan Seuntjens, Norma Ybarra","doi":"10.1080/09553002.2025.2523294","DOIUrl":"https://doi.org/10.1080/09553002.2025.2523294","url":null,"abstract":"<p><strong>Purpose: </strong>Radiotherapy (RT) is a standard treatment for non-small cell lung cancer (NSCLC). Radiosensitizers enhance radiation-mediated cancer cell elimination but lack selectivity and therefore also enhance normal tissue damage. L-carnosine (CAR) shows promise, having selective radiation-enhancing properties in vitro. This study validates CAR's selective radiation-enhancing properties in vivo, resulting in reduced tumor volume without causing damage to normal lung tissues.</p><p><strong>Methods: </strong>An orthotopic NSCLC model was established by implanting NCI-H1299 cells into male and female athymic nude mice. Mice were randomly divided into four treatment groups: (1) Control, (2) RT-only, (3) CAR-only and (4) CAR+RT. Control and RT-only received 8 days of intraperitoneal vehicle, while CAR-only and CAR+RT received 1 M CAR (500 μL/day) intraperitoneally for 8 days. A single 20 Gy RT dose was delivered to RT-only and CAR+RT treated mice after 4-days of CAR treatment. The response of tumors to treatment was evaluated using CT imaging and immunohistochemistry (IHC), and the effects on normal lung tissue were evaluated using IHC.</p><p><strong>Results: </strong>CAR+RT significantly reduced tumor volumes and reduced expression of tumor aggressiveness markers without increasing damage to the normal lung tissue when compared to RT-only group in both sexes.</p><p><strong>Conclusion: </strong>Treatment with CAR in combination with RT significantly reduces tumor volume and cancer cell proliferation in vivo without affecting normal lung tissue. Our study supports CAR's potential as a safe and selective radiation-enhancer that could widen the therapeutic window of RT.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative impact of fractionated and acute doses of gamma irradiation on the rats' brain after whole body irradiation.","authors":"Manal Mohammed, Azza El-Bahkery, Shereen Shedid","doi":"10.1080/09553002.2025.2523287","DOIUrl":"10.1080/09553002.2025.2523287","url":null,"abstract":"<p><strong>Purpose: </strong>Understanding the distinct impacts of fractionated versus acute doses of whole body irradiation on the brain and how these contrasting dosing schedules affect the brain at a cellular and molecular level.</p><p><strong>Materials and methods: </strong>Thirty healthy rats (males) were separated into five groups. Groups I, III, and V were irradiated by 0 Gy, 6 Gy, and 8 Gy of whole body γ-irradiation, respectively. Groups II and IV were irradiated by 6 Gy and 8 Gy as fractionated doses/4 (24-hour interval), respectively. Twenty-four hours after irradiation the total antioxidant capacity (TAC), comet assay, phosphorylated tau (p-tau) protein, caspase-3, transforming growth factor-β (TGF-β), microtubule-associated protein tau (MAPT) and protein kinase-N (PKN) gene expressions, and doublecortin (DCX) were measured.</p><p><strong>Results: </strong>The present study indicated varying levels of TAC, DNA damage, p-tau, caspase-3, and DCX, based on the dosing strategy employed. Acute doses resulted in greater DNA damage and early molecular indicators associated with neurodegenerative pathway compared to fractionated doses. Changes in PKN and MAPT gene expressions were also observed, suggesting genetic responses to radiation exposure; however, the acute irradiation did not significantly induce the gene expression changes compared to the respective fractionated group. In a different manner, TGF-β at (8 Gy) showed significant unique decrease in contrast to the substantial increase after exposure to (8 Gy/4) irradiation.</p><p><strong>Conclusions: </strong>There is a potential effect of the dosing strategy on brain's antioxidant defense system's response to oxidative stress, DNA, apoptotic pathway, neuronal function and structure, and neurogenesis. TGF-β results underscore its signaling in mediating the brain's response to irradiation-induced damage.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability of radiation doses reconstructed by EPR in teeth of former United States nuclear workers.","authors":"Alexander Romanyukha, Keith Consani, Sergey Y Tolmachev","doi":"10.1080/09553002.2025.2519523","DOIUrl":"https://doi.org/10.1080/09553002.2025.2519523","url":null,"abstract":"<p><strong>Purpose: </strong>Our prior results showed that in the most cases, radiation doses measured by electron paramagnetic resonance (EPR) in tooth enamel samples significantly exceeded worksite reported doses. In an effort to understand causes of this discrepancy, we carried out EPR dose measurements in additional tooth samples collected from individuals studied before.</p><p><strong>Materials and methods: </strong>Tooth enamel samples from five tissue donors to the United States Transuranium and Uranium Registries were used in this study. EPR measurements were performed using ELEXYS 500 spectrometer and high purity germanium detectors were used to measure gamma-emitting radionuclides.</p><p><strong>Results: </strong>Significant variation of the EPR measured doses among multiple teeth collected from the same individuals was observed. These variations are potentially due to an additional exposure of the head/neck region as compared to the other parts of the body, e.g. torso where personal dosimeters are typically worn. The latter could explain very significant discrepancy of the doses, derived from EPR measurements and reported by worksites. With gamma-spectroscopy, no ¹³⁷Cs was detected in tooth roots.</p><p><strong>Conclusions: </strong>In several cases there was nonuniform exposure of the head of the teeth' donors which may explain the discrepancy between worksite reported and EPR reconstructed doses. Results of the gamma counting suggested that exposure from ¹³⁷Cs in the roots was not a factor in the observed discrepancy.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized combination methods for exploring novel space environment-responsive genes and their roles: insights from space-flown <i>C. elegans</i> and their implications for astronauts.","authors":"Zejun Li, Lei Zhao, Ge Zhang, Dong Mi, Yeqing Sun","doi":"10.1080/09553002.2025.2519561","DOIUrl":"https://doi.org/10.1080/09553002.2025.2519561","url":null,"abstract":"<p><strong>Purpose: </strong>By expanding the catalog of spaceflight-induced molecular signatures in <i>Caenorhabditis elegans</i>, we aim to identify key molecular features and potential mechanisms underlying space environment-induced health risks to astronauts using <i>C. elegans</i> as a model organism.</p><p><strong>Methods and materials: </strong>We employed an optimized combination algorithm that integrated two co-expression network analysis methods and four machine learning-based models to identify space environment-responsive genes (SEGs) in space-flown <i>C. elegans</i>. The expression levels and associated biological processes of human orthologues of identified <i>C. elegans</i> genes were further analyzed using data from the JAXA CFE and NASA Twins studies.</p><p><strong>Results: </strong>A total of 114 SEGs that were implicated in four biological processes, including DNA repair, metabolic process, reproductive and developmental process, and lifespan regulation in space-flown <i>C. elegans</i>. We obtained 19 SEGs as potential indicators associated with health risks of the space environment. Further, the human orthologues of <i>C. elegans</i> SEGs that also exhibited altered expression in the blood samples of astronauts.</p><p><strong>Conclusions: </strong>This study provides new insights into the molecular mechanisms behind spaceflight-induced health risks and highlights potential mechanistic targets for preventive measures. The findings suggest a conserved genetic response to space conditions between <i>C. elegans</i> and astronauts, offering valuable targets for mitigating the health risks of space exploration.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-21"},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-dose ionizing radiation increases the lifespan of <i>Drosophila melanogaster</i> under oxidative stress conditions through AKT/SOD regulation.","authors":"Seon Young Nam, Haemin Jeong","doi":"10.1080/09553002.2025.2502018","DOIUrl":"https://doi.org/10.1080/09553002.2025.2502018","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the biological effects of low-dose ionizing radiation (LDIR) on living organisms, we analyzed the effects of LDIR of 0.05 Gy and its mechanism on the <i>Drosophila melanogaster,</i> which is an ideal model organism for aging studies due to its short lifespan and conserved molecular pathways.</p><p><strong>Materials and methods: </strong>The embryos of <i>Drosophila melanogaster</i> were irradiated and we analyzed the effects of LDIR treatment on the lifespan of <i>Drosophila melanogaster</i>. To investigate the effects of LDIR under oxidative stress conditions, because oxidative stress is a major cause of aging, reactive oxygen species (ROS) levels and the oxidative stress susceptibility of flies under hydrogen peroxide (H₂O₂)-induced oxidative stress conditions were evaluated at early post-irradiation time points. We next elucidated the effects of LDIR on the molecular mechanisms of lifespan extension.</p><p><strong>Results: </strong>LDIR extended the lifespan, but did not alter developmental rates. In addition, the locomotive deterioration of aged flies was rescued by exposure to a 0.05 Gy dose of γ-irradiation. LDIR increased the survival rates and locomotive ability under oxidative stress conditions and decreased mRNA levels of the pro-apoptotic factors, <i>grim</i> and <i>reaper</i>. Moreover, 0.05 Gy of LDIR decreased ROS accumulation and increased superoxide dismutase (SOD) activity in flies exposed to H₂O₂. Also, LDIR activated AKT pathway in H₂O₂-treated flies and inhibition of AKT activity suppressed the effects of LDIR.</p><p><strong>Conclusions: </strong>LDIR of 0.05 Gy extends the lifespan and survival rates under H₂O₂-induced oxidative stress conditions by controlling ROS levels via AKT activation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}