International journal of radiation biology最新文献

{"title":"Cohort methods: the CANadian RADiation workers (CANRAD) cohort.","authors":"Rachel S D Lane, Tim Prendergast, Laura A Rodriguez-Villamizar, Phillippe Prince, Lydia B Zablotska","doi":"10.1080/09553002.2026.2733660","DOIUrl":"10.1080/09553002.2026.2733660","url":null,"abstract":"<p><strong>Purpose: </strong>The long-term follow-up of workers with protracted exposure to low dose occupational ionizing radiation is important to understand the health effects and risks of radiation. This paper describes the creation and record linkage of the CANadian RADiation workers (CANRAD) cohort, including the work to resolve data issues and linkage challenges to outcome databases.</p><p><strong>Materials and methods: </strong>The CANRAD cohort includes all workers with occupational exposure to ionizing radiation (1951-2018) in Canada's National Dose Registry (NDR) and three facility-based sub-cohorts: Eldorado uranium workers (1932-1980), Ontario uranium miners (1954-1996), and Atomic Energy of Canada Limited nuclear workers (1956-1985). The sub-cohorts were added to the NDR to update workers' employment histories and radiation exposures to 2018. Deterministic and probabilistic record linkage methods linked the CANRAD cohort to national population-based mortality (1950-2020), cancer incidence (1969-2019), and tax (1981-2020) data.</p><p><strong>Results: </strong>Of 957,580 CANRAD workers, 883,150 (92.2%) successfully linked to Statistics Canada vital statistics databases. The analytical cohort includes 860,650 (89.9%) workers, including 427,920 (49.7%) females. At the end of follow-up, 751,520 (87.3%) of the cohort were alive and 94,410 (11.0%) were dead. Neoplasms and circulatory disease represented 63.8% (57,030) of all known causes of death (89,330). Prostate, breast, lung & bronchus, and colorectal cancer represented 49.9% (40,780) of first primary cancers (81,670). The mean cumulative effective dose was 17.1 mSv; but most cumulative doses were <10 mSv (∼85.0% of males and 98.1% of females). Females (350,510, 82.4%) dominated the medical sector.</p><p><strong>Conclusions: </strong>The CANRAD cohort is a large and valuable cohort of Canadian occupationally exposed radiation workers with employment histories, radiation exposures and long-term follow-up of tax, mortality, and cancer incidence. Analysis of this cohort will support the radiation protection framework.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a small ('silent') rate beta-irradiation field on the response of human neutrophil outer zeta potential to adrenergic and adenosinergic ligands.","authors":"Victor Zhirnov, Igor Iakovenko, Volodymyr Brovarets","doi":"10.1080/09553002.2026.2682846","DOIUrl":"https://doi.org/10.1080/09553002.2026.2682846","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to investigate the zeta potential (ZP) response of human neutrophils to well-characterized ligands of adrenergic and adenosine receptors under a small rate β-radiation (SRβR) field.</p><p><strong>Materials and methods: </strong>The cells were exposed to ionizing radiation by adding ¹⁴C-leucine to the cell incubation medium at dose rates ranging from 0.01 to 100 <i>μ</i>Gy·h^-1 at 10-fold intervals over 1 h. The ZP was calculated from the electrophoretic mobility value using Zeta for Windows.</p><p><strong>Results and conclusions: </strong>As the dose rate increases, the surface negative charge of neutrophils also increases, and the curve passes through a maximum at 10 μGy·h^-1. It was shown that the SRβR increases neutrophil ZP due to the dose rate, rather than the absorbed dose, and depends on the interaction of the tested ligands with the plasma membrane as a whole, and not only with the targets of these ligands. Most ligands induced unidirectional changes in ZP within the applied concentrations, the direction of which was ligand-dependent. Only yohimbine showed concentration-dependent, oppositely directed ZP changes. Most ZP responses to ligands were modified by the radiation. The data obtained indicate that alteration of the response of the outer layer of the plasma membrane of human neutrophils to receptor ligands under the influence of a small rate radiation field can alter the ZP-dependent immune functions of neutrophils, at least in vitro.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honoring Miral Dizdaroglu: the dizdar, a lesion-resolved unit for quantifying radiation-induced DNA damage.","authors":"Joseph W F Robertson, Michael Zwolak, Michael Lamontagne, Tadas Penkauskas, Ronald E Tosh, Paul Patrone, Jerimy C Polf, Ileana M Pazos","doi":"10.1080/09553002.2026.2730052","DOIUrl":"https://doi.org/10.1080/09553002.2026.2730052","url":null,"abstract":"<p><strong>Purpose: </strong>We propose a unit-the dizdar, <math><mrow><mtext>Dz</mtext></mrow></math>-defined as the number of lesions per kilogram of DNA. In biomedical and exposure settings, this would provide a common unit for reporting irradiation-induced lesion burdens, complementing absorbed dose. The dizdar is named in honor of Dr. Miral Dizdaroglu, whose pioneering work established rigorous chemical identification and quantitative measurement of oxidative and radiation-induced DNA lesions. We give an overview of the foundation he laid for DNA damage and discuss how this naturally leads to a vector of quantities, <math><mrow><mi>D</mi><mo>=</mo><msub><mrow><mo>(</mo><msub><mi>D</mi><mi>z</mi></msub><mo>)</mo></mrow><mi>z</mi></msub><mo>,</mo><mtext> with,</mtext> <msub><mi>D</mi><mi>z</mi></msub><mo> </mo><mo>=</mo><mo> </mo><mfrac><mrow><msub><mi>N</mi><mi>z</mi></msub></mrow><mrow><msub><mi>n</mi><mrow><mtext>DNA</mtext></mrow></msub></mrow></mfrac></mrow></math> indexed by lesion class <i>z</i>. This complements absorbed dose (Gy) by capturing the primary molecular drivers of biological outcomes. Building on Miral's legacy and on this concept of lesion quantification, we are developing a new methodology that uses nanopore resistive pulse sensing to rapidly and quantitatively measure DNA lesions.</p><p><strong>Conclusion: </strong>This approach provides a basis for time-dependent measurements of DNA damage and, with further validation, could help assess repair dynamics and the biological effects of different radiation delivery modalities. Advances in single-molecule methods, uncertainty quantification, & calibration can help bridge dosimetry to biological effectiveness via the dizdar, quantifying one link in the stochastic chain that leads to cell death and cancer. In turn, this may deepen our understanding of radiation-induced DNA damage and have applications in emergency response, radiation processing, space research, & cancer treatment.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-15"},"PeriodicalIF":2.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of early radiation-responsive long non-coding RNAs in human lymphoblastoid cells AHH-1 and a preliminary investigation of their potential for biodosimetry.","authors":"Ting-Ting Zhang, Shuang Li, Qiang Liu, Qing-Chi Dai, Xue-Lei Tian, Qing-Jie Liu","doi":"10.1080/09553002.2026.2739661","DOIUrl":"https://doi.org/10.1080/09553002.2026.2739661","url":null,"abstract":"<p><strong>Purpose: </strong>In nuclear and radiological emergencies, rapid and high-throughput screening of victims is essential for effective medical treatment. The biodosimeter based on transcriptomics holds great promise in triaging vast casualties fast by microarray and PCR. We aimed to screen early radiation-responsive long non-coding RNAs (lncRNAs) and preliminarily explore their potential for biodosimetry.</p><p><strong>Methods: </strong>We screened radiation differentially expressed (DE) lncRNAs through whole transcriptome sequencing in AHH-1 cells at 4 h post-irradiation. The dose-response relationships for early radiation-responsive lncRNAs were confirmed at 4, 12, 24 and 48 h after exposure to 0, 1, 2, 4, 6, 8 and 12 Gy γ-rays by real-time polymerase chain reaction (real-time qPCR). Four lncRNAs including lnc-INTS8-5:3, MIR34AHG:10, PVT1:58 and LINC01759:20, were further validated using ex vivo irradiated peripheral whole blood from 15 healthy volunteers aged 20-29 at 4 h and 24 h post-exposure. LncRNA-based dose classification models were developed and verified by logistic regression and receiver operating characteristic (ROC) analysis.</p><p><strong>Results: </strong>Fifteen early radiation-responsive lncRNAs were recognized in AHH-1 cells, of which 12 demonstrated a good dose-response relationship. lnc-INTS8-5:3, MIR34AHG:10, PVT1:58 and LINC01759:20 were significantly upregulated in ex vivo irradiated human blood. Notably, LINC01759:20 had extremely low spontaneous abundance but demonstrated a significant fold change of up to 65 after irradiation. The dose classification models can effectively classify '0 Gy vs. >0 Gy' and '<2 Gy vs. ≥2 Gy' (AUC = 0.806-1.000). The excellent performance of the built models in judging whether exposed or not was confirmed by leave-one-donor-out cross-validation (LODO-CV).</p><p><strong>Conclusions: </strong>Ionizing radiation (IR) can rapidly induce dose-dependent expression changes of lncRNAs in human lymphoblastoid cells AHH-1. Furthermore, certain early and highly radiation-responsive lncRNAs in human whole blood show promise as preliminary candidate biomarkers for radiation exposure.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":2.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149120816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small modular reactors and our ecosystems - engagement, transparency & trust; summary of a workshop held at McMaster University, Canada.","authors":"Rhea Desai, Andrea Bonisoli-Alquati, Paul N Schofield, James LeMoine, Lawrence A Kapustka, Kentaro Ariyoshi, Soo Hyun Byun, Adrienne Ethier, Stephen Marshall, Colin Seymour, Trevor J Stocki, Deborah H Oughton, Carmel Mothersill","doi":"10.1080/09553002.2026.2730059","DOIUrl":"https://doi.org/10.1080/09553002.2026.2730059","url":null,"abstract":"<p><p><b>Purpose:</b> Globally, governments are launching national programs, demonstration projects and industrial partnerships to bring small modular reactors (SMR) to market as lower-carbon, more flexible forms of nuclear power. Current international assessments suggest the first wave of commercial projects will be built in the 2020s, followed by broader deployment. It is anticipated that SMR could provide roughly a quarter of new nuclear capacity added worldwide by 2050. Siting of reactors is expected to be distributed and distinct from traditional large-scale dedicated centers; for example, close to cities and in remote environments. Unique safety concerns exist for these sites, and concerns go beyond solely traditional radiation protection and involve the impacts of infrastructure on site which extend beyond concerns associated with conventional large nuclear facilities. These include cradle-to-grave assessment of uranium mining, milling and transport, worker accommodation, and both human and environmental disruption. Waste associated with cooling, operation, and decommissioning is also an issue. This requires monitoring potential sites appropriately to establish comprehensive ecosystem baselines. <b>Conclusions:</b> This paper summarizes and expands the discussion of a group of experts who gathered to discuss the environmental risks and potentials of SMR in addressing Canada's climate goals, energy security, industrial and economic development. Experts were from areas including radiobiology, radioecology, radiation protection, engineering, regulatory authority and Indigenous community leadership. Discussions focused on scientific and governance principles that should inform the potential proliferation of multiple SMR, including design, siting, and assessment of their environmental impacts. This paper is derived from discussions among participating experts and reflects perspectives expressed by various contributors. While the paper aims to synthesize key themes that emerged during discussions, it does not represent the views of all participants, nor does it reflect a consensus position. Views expressed should not be interpreted as official positions of any participating organization, institution, or regulatory body.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149032130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation-induced chromosomal aberrations in pediatric patients with inborn errors of immunity: a G₀-phase approach to assess radiosensitivity.","authors":"Usha Yadav, Utkarsha S Mungse, Pallavi Gaikwad, Arshad Khan, Balvinder K Sapra, Umair Ahmed Bargir, Manisha Madkaikar, Nagesh N Bhat","doi":"10.1080/09553002.2026.2733665","DOIUrl":"https://doi.org/10.1080/09553002.2026.2733665","url":null,"abstract":"<p><strong>Background: </strong>Understanding the radiosensitivity status of patients may provide crucial insight for planning personalized treatment when high doses of ionizing radiation are part of treatment regimen. This is particularly relevant in case of pediatric patients who are to undergo myeloablative conditioning prior to hematopoietic stem cell transplantation (HSCT). Assessment of cytogenetic aberrations following irradiation using conventional blood culture is standard approach for evaluating radiosensitivity. However, in certain disorders, cells often exhibit poor proliferation, leading to culture failure. In such instances, aberration assessment with G₀-phase premature chromosome condensation (G₀-PCC) without culture may provide a suitable alternative.</p><p><strong>Purpose: </strong>In the current study, we have assessed baseline and radiation induced chromosome aberrations among three pediatric candidates for HSCT who were diagnosed with rare inborn errors of immunity disorders; Fanconi anemia (FA), leukocyte adhesion deficiency-I (LAD I) and Wiskott-Aldrich syndrome (WAS) using the G₀-PCC with fluorescence in situ hybridization (G₀-PCC-FISH) assay.</p><p><strong>Methodology: </strong>Blood samples were exposed to 2 Gy gamma radiation. Baseline and radiation-induced chromosomal aberrations were quantified with G₀-PCC-FISH and compared with baselines from healthy controls.</p><p><strong>Results: </strong>All three cases demonstrated elevated radiation-induced aberration frequencies, indicating increased radiosensitivity and impaired DNA repair capacity.</p><p><strong>Conclusions: </strong>These findings highlight the potential utility of G₀-PCC-FISH as a rapid tool for radiosensitivity assessment. Especially useful when metaphases are difficult obtain due to failing cultures.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":2.4,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149032092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to pulse modulated radiofrequency electromagnetic fields does not affect Sternberg working memory performance.","authors":"Adam Verrender, Anna Dalecki, Sarah P Loughran, Abigail Duff, Jennifer L Shore, Rodney J Croft","doi":"10.1080/09553002.2026.2733658","DOIUrl":"https://doi.org/10.1080/09553002.2026.2733658","url":null,"abstract":"<p><strong>Purpose: </strong>To replicate a previously reported finding that radiofrequency electromagnetic field (RF-EMF) exposure improves performance on the Sternberg working memory task, as indicated by decreased reaction time, and to test whether this effect, if verified, can also be induced by exposure to an infrared electromagnetic field (IR-EMF).</p><p><strong>Materials and methods: </strong>Forty-eight healthy adults participated in a randomized, counterbalanced provocation study. Working memory performance was assessed using a modified Sternberg working memory task, which was calibrated to individual performance levels in a preliminary testing session. An sXh920 planar exposure system was used to generate a 920 MHz GSM-like signal, providing two double-blind conditions (peak-spatial SAR average over 10 g) of 0 W/kg (Sham) and 2 W/kg (RF-EMF). A standard, 'off the shelf' 250 W infrared globe provided an IR-EMF exposure with an equivalent heating profile over the skin as that due to the RF-EMF exposure (circa 0.3 °C).</p><p><strong>Results: </strong>RF-EMF exposure did not reduce reaction time in the Sternberg working memory task. Exploratory tests also revealed there was no effect of IR-EMF on reaction time, and no other performance measures (response bias and response discrimination) were affected by either RF-EMF or IR-EMF exposure.</p><p><strong>Conclusions: </strong>The current study found no evidence that acute exposure to RF-EMF decreases reaction time on a Sternberg working memory task. The failure to replicate our previously reported effect of RF-EMF exposure on reaction time indicates that our initial finding was not an effect of RF-EMF exposure and was likely due to chance.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-8"},"PeriodicalIF":2.4,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149026890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring how metabolism comes into play with radiations: a review.","authors":"Damian Bimbenet, Mathilde Badoual, Gibin Powathil, Angélique Stéphanou","doi":"10.1080/09553002.2026.2730057","DOIUrl":"https://doi.org/10.1080/09553002.2026.2730057","url":null,"abstract":"<p><strong>Purpose: </strong>Ionizing radiation consists in the emission of photons such as X- and <math><mrow><mi>γ</mi></mrow></math>-rays or particles like carbon ions or <math><mrow><mi>α</mi></mrow></math>-particles. Glucose metabolism, encompassing glycolysis and oxidative phosphorylation, plays a central role in cellular energy production. Emerging evidence reveals a complex and reciprocal relationship between ionizing radiation and glucose metabolism, with significant implications for radiotherapy outcomes. This review provides a comprehensive overview of the molecular and cellular interactions between these two processes, highlighting both historical findings and recent advances.</p><p><strong>Conclusions: </strong>Irradiation induces transient increases in glycolytic enzyme activity and temporary suppression of mitochondrial respiration, suggesting a coordinated metabolic response. Reactive oxygen species (ROS) and HIF-1<math><mrow><mi>α</mi></mrow></math> appear as central regulators of this response, though their dynamics remain a topic of ongoing investigation. Importantly, a high glycolytic phenotype is consistently associated with increased radioresistance and poor clinical prognosis. Furthermore, glucose metabolism intersects with other modulators of radiosensitivity, including cancer stem cells and cell-cycle regulation. By synthesizing key biological insights, this review lays a foundation for future in silico modeling efforts aimed at understanding and potentially overcoming treatment resistance through metabolic targeting.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":2.4,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148972733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation and comparison of the buccal epithelial micronuclei count among the population residing around the cellular towers with varying power density.","authors":"John Baliah, Balanehru Subramanian, Vidyalakshmi Santhanam, David Livingstone","doi":"10.1080/09553002.2026.2730050","DOIUrl":"https://doi.org/10.1080/09553002.2026.2730050","url":null,"abstract":"<p><strong>Purpose: </strong>Continuous whole-body radiation exposure from cellular towers may constitute a health risk that needs scientific evaluation. Micronuclei (MN) are cytoplasmic chromatin masses indicating genotoxicity that arise from chromosome fragments or due to a lag in the anaphase of whole chromosomes. The purpose of this study is to estimate and compare the MN count among the population residing around cellular towers with low, medium, and high-power density.</p><p><strong>Materials and methods: </strong>Healthy participants (<i>n = 60</i>) of both genders, aged between 18 and 30 years, residing around the cellular towers, constituted the study population. The classification of power density (PD) into three study groups, namely low, medium, and high, was created with 20 participants in each group. The buccal epithelial smear was taken with a cytobrush, fixed, and Papanicolaou (PAP) stained for counting the MN. Two experienced oral pathologists counted the MN with substantial agreeability. ANOVA was used for intergroup comparison between low, medium, and high-PD groups for MN count. Post-hoc Bonferroni correction was used for intergroup comparisons. SPSS version 25.0 was used for the statistical analysis.</p><p><strong>Results and conclusions: </strong>A statistically meaningful difference in the MN is observed (<i>P =</i> 0.018) among the three groups. On comparison to the low-PD group, there is a statistically meaningful increase (<i>P =</i> 0.02) in the MN count in the medium-PD group. Further, a non-significant increase in MN count is observed in the high-PD group. The preliminary observational nature of the finding indicates a non-monotonic dose-response relationship and is merely associative on buccal epithelial MN count.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-6"},"PeriodicalIF":2.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionizing radiation induces bidirectional transcriptomic reprogramming and dynamic NOS2/TREM2 regulation in triple-negative breast cancer cells.","authors":"Jing Li, Xin Bai, Wanjia Qiao, Yong Yang, Zhaobing Gao, Haiwen Liang, Lingfang Zhang, Biaofeng Shan, Jianming Tang","doi":"10.1080/09553002.2026.2730060","DOIUrl":"https://doi.org/10.1080/09553002.2026.2730060","url":null,"abstract":"<p><strong>Purpose: </strong>To characterize irradiation-associated transcriptomic changes in murine triple-negative breast cancer cells and examine dose- and time-response patterns of selected radiation-responsive candidates.</p><p><strong>Materials and methods: </strong>RNA sequencing (RNA-seq) was performed in 4T1 cells collected 24 h after 4 Gy irradiation, followed by Reactome and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and gene set enrichment analyses. Representative RNA-seq-derived genes were examined by reverse transcription quantitative PCR (RT-qPCR), and selected immune- and inflammation-related transcripts were further assessed across additional radiation doses and post-irradiation time points. Inducible nitric oxide synthase (NOS2) and triggering receptor expressed on myeloid cells 2 (TREM2) protein abundance was assessed by Western blotting, and nitrite accumulation in culture supernatants was measured using a Griess reagent-based assay as an indirect readout of nitric oxide production.</p><p><strong>Results: </strong>RNA sequencing identified 757 differentially expressed genes, including 285 upregulated and 472 downregulated genes. Irradiation was associated with enrichment of inflammatory, interferon-related, immune-system, and cell-adhesion transcriptional signatures, whereas downregulated genes were enriched in cell-cycle-, chromosome-cohesion-, DNA-damage-response-, DNA-repair-, and SUMOylation-related pathways. Selected immune- and inflammation-related transcripts showed distinct temporal patterns. Nos2 mRNA increased across the examined 0-6 Gy dose range and at later post-irradiation time points, whereas NOS2 protein showed different kinetics, with an early peak after 4 Gy irradiation and no clear further increase above 6 Gy. Nitrite accumulation increased after irradiation. Trem2 showed the largest fold increase among strongly upregulated transcripts identified by RNA-seq, but RT-qPCR detected a significant increase only at 24 h, and TREM2 protein abundance remained unchanged across the examined doses and time points.</p><p><strong>Conclusions: </strong>Ionizing radiation was associated with broad bidirectional transcriptional remodeling in 4T1 cells, involving immune-, inflammatory-, and interferon-related signatures together with reduced representation of cell-cycle- and DNA-repair-related gene sets. The discordant mRNA and protein patterns of NOS2 and TREM2 indicate that transcript-level responses do not necessarily translate into corresponding protein-level changes. These findings define irradiation-associated molecular responses requiring further functional investigation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-12"},"PeriodicalIF":2.4,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}