International journal of radiation biology最新文献_第2页

The impact of pre-radiation exposure on physiological and fermentation activity of Saccharomyces cerevisiae SAF Ale S-33. 辐照前暴露对酿酒酵母SAF Ale S-33生理和发酵活性的影响。

International journal of radiation biology Pub Date : 2025-05-07 DOI: 10.1080/09553002.2025.2498979

Emmanuel Kormla Danyo, Maria Igorevna Tokareva, Ruslan Albertovich Vazirov, Irina Stanislavovna Selezneva

{"title":"The impact of pre-radiation exposure on physiological and fermentation activity of Saccharomyces cerevisiae SAF Ale S-33.","authors":"Emmanuel Kormla Danyo, Maria Igorevna Tokareva, Ruslan Albertovich Vazirov, Irina Stanislavovna Selezneva","doi":"10.1080/09553002.2025.2498979","DOIUrl":"https://doi.org/10.1080/09553002.2025.2498979","url":null,"abstract":"Purpose: The aim of this study was to evaluate the effect of radiation pre-exposure on the survival, physiological, and fermentation activity of Saccharomyces cerevisiae.Materials and methods: The low-dose (20-80 cGy) irradiation was conducted using the medical therapy installation Xstrahl 320, which has a maximum energy of 320 keV and a dose rate of 1 Gy/min. High-dose (1 and 5 kGy) exposure was achieved by an electron beam using the linear accelerator UELR-10-10S with a maximum energy of 10 MeV and a dose rate of 1 kGy/s.Results: The results showed that hyper-radiosensitivity of the yeast exposed to low-dose radiation occurred at 20 cGy and induced radio-resistance occurred at doses from 40 to 60 cGy, increasing survival. The pre-exposure of S. cerevisiae to 60 cGy radiation conferred adaptation on the yeast to withstand repeated exposure to a higher dose (1 kGy). The irradiation of S. cerevisiae with low doses ranging from 20 to 80 cGy decreased its physiological and metabolic activity compared to the non-irradiated yeast samples. The viability of the yeast was totally lost after exposure to 5 kGy, resulting in the death of all the yeast.Conclusions: The pre-exposure of S. cerevisiae to radiation resulted in a decline in growth, vitality, metabolic activity, and fermentation activity. However, pre-exposure of the yeast to low-dose radiation (40 and 60 cGy) induced an adaptive response, reducing the damaging effect usually experienced from high-dose (kGy) exposure.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transdisciplinary team-based cancer research: a model for training the next generation of cancer researchers. 基于跨学科团队的癌症研究：培养下一代癌症研究人员的模式。

International journal of radiation biology Pub Date : 2025-05-06 DOI: 10.1080/09553002.2025.2494546

Dietmar W Siemann, Wendy Malorzo

{"title":"Transdisciplinary team-based cancer research: a model for training the next generation of cancer researchers.","authors":"Dietmar W Siemann, Wendy Malorzo","doi":"10.1080/09553002.2025.2494546","DOIUrl":"https://doi.org/10.1080/09553002.2025.2494546","url":null,"abstract":"Purpose: Interdisciplinary team collaboration is a key element of discovery and advancement in oncology science and medicine. This fact is well-recognized by both laboratory and physician scientists and yet, most cancer research trainees are minimally exposed to and ill prepared in the collaboration skills required for them to be successful in the future. Similarly, near-peer mentoring, the benefits of which have been broadly published for precollegiate and undergraduate programs, is usually not a formal training component for doctoral and postdoctoral oncology trainees. At the University of Florida, the Team-based Interdisciplinary Cancer Research Training (TICaRT) Program seeks to address this unmet need by offering a unique opportunity for trainees to develop interdisciplinary skills in cancer research and gain collaborative expertise through team-based learning and near-peer mentoring.Conclusion: Active transdisciplinary research collaboration drives cancer discovery, knowledge, and treatment. Providing opportunities for trainees to participate in collaborative research experiences by incorporating team science and near-peer mentoring concepts as integral components of their training would greatly aid their preparation to become successful next-generation cancer researchers.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of dosimetric parameters reporting in biological dosimetry peer-reviewed publications: strengths and weaknesses. 生物剂量学同行评审出版物中剂量学参数报告的分析：优点和缺点。

International journal of radiation biology Pub Date : 2025-05-05 DOI: 10.1080/09553002.2025.2494551

Morgane Dos Santos, François Trompier

{"title":"Analysis of dosimetric parameters reporting in biological dosimetry peer-reviewed publications: strengths and weaknesses.","authors":"Morgane Dos Santos, François Trompier","doi":"10.1080/09553002.2025.2494551","DOIUrl":"https://doi.org/10.1080/09553002.2025.2494551","url":null,"abstract":"Purpose: Dosimetry reporting in radiobiological studies is frequently incomplete and leads to poor replicability. As the main objective of biological dosimetry is estimating an exposure dose reflecting a whole-body dose equivalent to assess acute and delayed health risks, dosimetry reporting is even more crucial. This study aims to evaluate the quality of the dosimetry reporting and irradiation parameters related to biological dosimetry publications.Material and methods: 114 publications over a period of 94 years focused on biological dosimetry studies were analyzed. Nineteen parameters distributed into four categories (source specification, irradiation geometry, calibration, and irradiation details) were scored to evaluate the quality of the dosimetry reporting.Results: We show that, even though parameters such as radiation type, sample type, and dose are systematically reported, and source type, energy/voltage, dose rate, and exposure type in more than 80% of publications, several crucial parameters are missing. Specifically, information regarding the dosimetry protocol, setup, and calibration are often not reported, making it impossible to reproduce the experiments.Conclusions: This study highlighted that certain parameter, such as the source used or the irradiation dose, are very often well-reported. Still, critical parameters such as the dosimetric quantity or the method used for dosimetric measurements have to be systematically added. Efforts already underway in this area must be continued to further improve the description of irradiation parameters for radiobiology studies.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of radiation resistance in colorectal cancer: in silico identification of AURKA, BIRC5 and PLK1 proteins as potential biomarkers. 结直肠癌放射耐药的分子机制：AURKA， BIRC5和PLK1蛋白作为潜在生物标志物的计算机鉴定

International journal of radiation biology Pub Date : 2025-04-28 DOI: 10.1080/09553002.2025.2496079

Marta Rodríguez-García, Antonio M Burgos-Molina, Alejandro González-Vidal, Francisco Sendra-Portero, Manuel Bernal, Miguel J Ruiz-Gómez

{"title":"Molecular mechanisms of radiation resistance in colorectal cancer: in silico identification of AURKA, BIRC5 and PLK1 proteins as potential biomarkers.","authors":"Marta Rodríguez-García, Antonio M Burgos-Molina, Alejandro González-Vidal, Francisco Sendra-Portero, Manuel Bernal, Miguel J Ruiz-Gómez","doi":"10.1080/09553002.2025.2496079","DOIUrl":"https://doi.org/10.1080/09553002.2025.2496079","url":null,"abstract":"Purpose: The development of radiation resistance by tumor cells severely affects the survival of colorectal cancer patients. The aim of this work is to study the molecular mechanisms involved in the resistance to radiotherapy treatment in colorectal cancer and the identification of key genes as possible biomarkers.Methods: Data mining was performed in PubMed with the keywords 'colorectal neoplasms', 'radiotherapy', and 'resistance', generating a total of 242 articles in which a series of inclusion and exclusion criteria were applied to select the articles of interest. Then, an in-silico analysis of the selected genes was performed with the bioinformatic tools: GeneCodis, Metascape, KEGG, REACTOME, STRING, STITCH, CHEA3, DGIdb, CTD, and GEPIA.Results: Different mechanisms and genes involved in radiation resistance were described. These are related to evasion of apoptosis, cell cycle dysregulation, epithelial-mesenchymal transition, and repair of DNA breaks, with the last one being the most relevant and influential. The In-silico study carried out with 21 genes involved in radiation resistance showed the implication of FoxO signaling and EGFR tyrosine kinase inhibitor resistance as the most enriched pathways. In addition, the study identified the key proteins AURKA, BIRC5, and PLK1, showing multiple interacting chemicals and drugs; such as tamoxifen, omacetaxine mepesuccinate, and hydroxyzine pamoate, among others.Conclusion: The identification of multiple transcription factors that regulate the expression of these key genes as well as the validation in patient samples where higher expression is observed in tumor patients, conserved across tumor stages I-IV, suggests their potential as possible biomarkers.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal gamma-ray doses for inducing mutation in cannabis sativa L. cultivars at different growth stages. 不同生育期诱导突变的最佳γ射线剂量。

International journal of radiation biology Pub Date : 2025-04-28 DOI: 10.1080/09553002.2025.2494609

Juyoung Kim, Dong-Gun Kim, Tae Hyun Ha, Jaihyuk Ryu, Ye-Jin Lee, Woon Ji Kim, Sang Hoon Kim

{"title":"Optimal gamma-ray doses for inducing mutation in cannabis sativa L. cultivars at different growth stages.","authors":"Juyoung Kim, Dong-Gun Kim, Tae Hyun Ha, Jaihyuk Ryu, Ye-Jin Lee, Woon Ji Kim, Sang Hoon Kim","doi":"10.1080/09553002.2025.2494609","DOIUrl":"https://doi.org/10.1080/09553002.2025.2494609","url":null,"abstract":"Purpose: Gamma (γ)-ray-dependent mutagenesis has not been fully established in cannabis. Hence, in this study, we aimed to determine the optimal γ-ray doses for inducing mutations in cannabis seeds and plants at the vegetative and reproductive stages.Materials and methods: The γ-irradiation ranging from 0 to 200 Gy was used to determine the optimal doses for three cannabis cultivars (CBGambit, Superwoman S1, Spectrum 303) at seeds, vegetative and reproductive stages. We examined several growth parameters (e.g., fresh weight, plant height, and number of leaves, seeds, and inflorescences) and performed linear regression analyses.Results: The optimal γ-ray dose by investigating growth parameters in seeds was determined to be 125 Gy (50% reduction dose, RD50) for 24 h. At the vegetative and reproductive stages, the optimal γ-ray doses were found to be 75 Gy and 55 Gy (RD50) for 24 h, respectively.Conclusions: Our findings will greatly progress the establishment of mutant cannabis populations and cannabis breeding research. Especially, growth parameters vary depending on the developmental stage of the cultivar, and corresponding RD50 doses can be applied accordingly. This detailed dose information will be helpful in cannabis mutation breeding to enhance desired traits such as specific cannabinoid content or biomass.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of polysaccharide POM extracted from Pleurotus ostreatus in curbing and treating the harmful effects on liver and kidney resulting from radiation exposure. 从褐藻中提取的多糖 POM 在抑制和治疗辐射照射对肝脏和肾脏造成的有害影响方面的作用。

International journal of radiation biology Pub Date : 2025-03-14 DOI: 10.1080/09553002.2025.2473979

Manal G Mahmoud, Mohsen S Asker, Mona A M Ghoneim, Amal I Hassan, Hala F Osman

{"title":"The role of polysaccharide POM extracted from Pleurotus ostreatus in curbing and treating the harmful effects on liver and kidney resulting from radiation exposure.","authors":"Manal G Mahmoud, Mohsen S Asker, Mona A M Ghoneim, Amal I Hassan, Hala F Osman","doi":"10.1080/09553002.2025.2473979","DOIUrl":"10.1080/09553002.2025.2473979","url":null,"abstract":"Purpose: This research assesses the radioprotective and antioxidant activity of polysaccharide (POM) derived from Pleurotus ostreatus strain on rats suffering from metabolic problems due to irradiation. The crude POM obtained through chemical analysis was found to consist of glucose, galactose, and fructose in 1:1.2:0.1 ratio but was absent of uronic acid, and sulfate. Moreover, it demonstrated radical scavenging activity (45.21-84.21%) at 5 mg/ml concentration.Methods: A total of thirty-two male Sprague-Dawley rats were used and split into four groups: control, radiation (whole body RT with 5 Gy gamma rays), POM treatment (28 days at 100 mg/kg body weight), POM + radiation (n = 8 rats/group). Monitoring of body weight was done and on day 28, cardiac blood samples were collected and tested on liver and kidney function indices. ELISA was used to test for inflammatory factors and liver and kidney sections were stained with hematoyxlin-eosin, and other tests for DNA fragmentation were undertaken.Results: The rats that underwent radiation exhibited reduced body weights, metabolic disruption, and decrease in antioxidant enzyme activities. The histological analysis displayed edema of hepatic cells, inflammatory cell infiltration, and vacuolar degeneration. On the other hand, rats in the POM + Radiation group showed less severe pathological changes. POM treated rats had restored their liver and kidney functions, lower TBARS, higher total protein, and improved antioxidant enzyme activities.Conclusion: POM treatment effectively alleviates liver and kidney damage caused by radiation exposure.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computed tomography-based pulmonary vasculature analysis of decreased lung perfusion after thoracic radiotherapy in patients with lung cancer. 肺癌患者胸部放疗后肺灌注减少的ct肺血管分析。

International journal of radiation biology Pub Date : 2025-01-01 Epub Date: 2024-12-02 DOI: 10.1080/09553002.2024.2435316

Yu-Sen Huang, Jenny Ling-Yu Chen, Wei-Chun Ko, Yee-Fan Lee, Yeun-Chung Chang

{"title":"Computed tomography-based pulmonary vasculature analysis of decreased lung perfusion after thoracic radiotherapy in patients with lung cancer.","authors":"Yu-Sen Huang, Jenny Ling-Yu Chen, Wei-Chun Ko, Yee-Fan Lee, Yeun-Chung Chang","doi":"10.1080/09553002.2024.2435316","DOIUrl":"10.1080/09553002.2024.2435316","url":null,"abstract":"Purpose: This study aimed to quantitatively assess changes in lung perfusion after thoracic radiotherapy in lung cancer patients.Materials and methods: Patients underwent chest computed tomography (CT) for pulmonary vasculature analysis before radiotherapy and at 3 and 12 months after radiotherapy. The correlation between the percentage decrease in lung perfusion after radiotherapy and the delivered radiotherapy dose was analyzed.Results: The ipsilateral lung, where the primary tumor was located, received a significantly higher dose than the contralateral lung (mean dose: 22.9 Gy vs. 6.8 Gy). At 3 months, significant reductions in lung perfusion parameters were observed in the ipsilateral lung (total blood volume (TBV): 13.8%, blood volume in vessels with cross-sectional areas of ≤10 mm2: 12.6%, blood volume in vessels with cross-sectional areas of ≤5 mm2: 11.7%, subpleural vessel count: 21.1%, subpleural vessel area: 16.9%, and subpleural vessel density: 12.3%). Significant negative correlations between perfusion parameters and the radiation dose delivered to the ipsilateral lung were observed. For every 1-Gy increase in the mean dose for the ipsilateral lung, TBV decreased by 0.852% (p = .044), and for every 1% increase in the percentage of lung volume that received more than 20 Gy, TBV decreased by 0.402% (p = .048). The 3-year overall survival of the patients was 75%. No significant association between baseline perfusion parameters and survival was observed.Conclusions: Thoracic radiotherapy significantly reduced pulmonary perfusion, especially in the ipsilateral lung. The reduction in perfusion correlated with the radiation dose. These findings underscore the impact of radiation-induced damage on perfusion.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"35-43"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examination of immunohistochemical of the effects of flattened and unflattened radiotherapy beams in nude mice breast cancer xenografts. 裸鼠乳腺癌异种移植术中放疗束平整化和非平整化效果的免疫组化观察。

International journal of radiation biology Pub Date : 2025-01-01 Epub Date: 2025-01-02 DOI: 10.1080/09553002.2024.2445582

Serhat Aras, Seyma Ozkanli, Engin Sumer, Tugba Kul Koprulu, Mustafa Efendioglu

{"title":"Examination of immunohistochemical of the effects of flattened and unflattened radiotherapy beams in nude mice breast cancer xenografts.","authors":"Serhat Aras, Seyma Ozkanli, Engin Sumer, Tugba Kul Koprulu, Mustafa Efendioglu","doi":"10.1080/09553002.2024.2445582","DOIUrl":"10.1080/09553002.2024.2445582","url":null,"abstract":"Purpose: The aim of this study was to investigate the radiobiological effects underlying the inhibition of breast cancer (BCa) following radiotherapy in nude mice models, and to evaluate the impact of changes in immunohistochemical parameters induced by FF and FFF beams.Materials and methods: The study included thirty-six adult nude mouse models, which were randomly assigned to five groups: control (G1), breast cancer (BCa) (G2), FF-400 MU/min (G3), FFF-1100 MU/min (G4), and FFF-1800 MU/min (G5). The control group received neither radiation nor treatment, while the BCa group had a cancer model without radiation. The BCa models were subjected to a single dose of 20 Gy of radiotherapy at varying dose rates. Twenty days after the implantation of the MCF-7 cancer cell line, the nude mice were irradiated and sacrificed 48 h later for ER, PR, HER-2, Ki-67, CD-133, Caspase-3, APAF-1, NOS-2 and NOS-3 IHC analysis.Results: A statistically significant decrease in IHC staining values for ER, Ki-67 and NOS-2 was observed in the FF-400 MU/min, FFF-1100 MU/min and FFF-1800 MU/min groups due to radiotherapy compared to the BCa group. The FFF beams demonstrated superior efficacy in the treatment of BCa. The significant differences in Caspase-3 and APAF-1 levels were found between BCa and control groups, while CD-133, NOS-3, HER-2, and PR staining showed no differences between groups.Conclusions: It was concluded that FFF beam was more effective than FF beam for BCa, especially on ER, Ki-67 and NOS-2 IHC parameters.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"264-273"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using ultrasound sequential images processing to predict radiotherapy-induced sternocleidomastoid muscle fibrosis. 应用超声序列图像处理预测放疗引起的胸锁乳突肌纤维化。

International journal of radiation biology Pub Date : 2025-01-01 Epub Date: 2025-02-03 DOI: 10.1080/09553002.2025.2453995

Asma Khodadadi, Manijhe Mokhtari-Dizaji, Reza Ghalehtaki, Niloofar Ayoobi Yazdi

{"title":"Using ultrasound sequential images processing to predict radiotherapy-induced sternocleidomastoid muscle fibrosis.","authors":"Asma Khodadadi, Manijhe Mokhtari-Dizaji, Reza Ghalehtaki, Niloofar Ayoobi Yazdi","doi":"10.1080/09553002.2025.2453995","DOIUrl":"10.1080/09553002.2025.2453995","url":null,"abstract":"Purpose: The purpose of this study was to evaluate the thickness and biomechanical parameters of the sternocleidomastoid muscle (SCM) before, during, and after radiotherapy using ultrasound elastography to predict radiotherapy-induced muscle fibrosis.Materials and methods: The mean daily absorbed doses of 20 SCMs were determined. To find out the Young and shear modulus, shear wave elastography (SWE) and the B-mode sequential images processing method were implemented. In the B-mode sequential images processing method, by administering dynamic stress, the Young and shear modulus were estimated utilizing the maximum gradient and the block-matching algorithms, respectively. The imaging was done before, during the third and sixth weeks of treatment, and 3 months after radiotherapy.Results: There was a statistically significant increase in the maximum thickness during the sixth week compared to before radiotherapy (p = .043). However, this parameter did not change significantly 3 months later (p = .095). The Young modulus (p = .611) derived from SWE did not differ significantly throughout any of the weeks of radiotherapy. But Young and shear modulus increased significantly in the B-mode sequential images processing method before and during the third and sixth weeks of treatment (p = .001). The outcomes observed 3 months after radiotherapy revealed a statistically significant increase in both Young modulus (p = .029) and shear modulus (p = .004) compared to pre-radiotherapy.Conclusion: The Young modulus and shear modulus are introduced as biological markers used to detect the onset of the fibrosis process during the initial radiotherapy fractions.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"411-421"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and verification of the dose-effect curve of dicentric chromosomes by semi-automatic analysis of human peripheral blood irradiated by X-ray in vitro. 体外x射线辐照人外周血半自动分析双中心染色体剂量效应曲线的建立与验证。

International journal of radiation biology Pub Date : 2025-01-01 Epub Date: 2025-04-28 DOI: 10.1080/09553002.2025.2494549

Yu Gao, Lin Han, Jie Li, Xue Lu, Ping Wang, Shasha Du, Yunfei Zhang, Tian-Jing Cai, Qing-Jie Liu, Yumin Lyu

{"title":"Establishment and verification of the dose-effect curve of dicentric chromosomes by semi-automatic analysis of human peripheral blood irradiated by X-ray in vitro.","authors":"Yu Gao, Lin Han, Jie Li, Xue Lu, Ping Wang, Shasha Du, Yunfei Zhang, Tian-Jing Cai, Qing-Jie Liu, Yumin Lyu","doi":"10.1080/09553002.2025.2494549","DOIUrl":"10.1080/09553002.2025.2494549","url":null,"abstract":"Objective: To establish and validate a dose-response curve for dicentric chromosomes (DC) induced by X-rays in human peripheral blood in vitro using semi-automated scoring.Methods: Peripheral blood samples were collected from three healthy individuals and exposed to X-ray doses of 0, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy at a dose rate of 1.158 Gy/min. Dicentric chromosomes in metaphase were scored both full- and semi-automatically, and a dose-response curve was generated with CABAS software based on dicentric yields. Dose estimations were then performed for 12 biodosimetry standard samples and one sample from a patient with X-ray-induced skin injury.Results: Dicentric yields increased with X-ray doses from 0 to 5 Gy (r = 0.943, P < 0.01). The dose-response association followed a linear-quadratic model: Y = 0.0002 (± 0.0001) + 0.0379 (± 0.0032) × D + 0.0253 (± 0.0014) × D2 (R2 = 0.998, P < 0.01), where Y represents dicentric yields and D is the dose. The estimated dose for 12 validation samples aligned closely with actual doses. The estimated whole-body average absorbed dose for the patient was 0.73 Gy, with observed DC over-dispersion suggesting partial body exposure. By using the Dolphin model, this dose was refined to 2.22 Gy and estimated irradiated body volume was 35.94%, consistent with clinical diagnosis.Conclusions: The dose-response curve developed using semi-automated scoring offer a reliable and efficient approach for dose estimation and clinical diagnosis in nuclear radiation emergencies. It could also support retrospective biodosimetry of partial-body, non-uniform radiation exposure.","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"590-597"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0