International journal of radiation biology最新文献

{"title":"Autophagy related proteins as potential biomarkers in predicting cancer prognosis after chemoradiotherapy: systematic review and meta-analysis.","authors":"Mozhdeh Zamani, Erfan Sadeghi, Pooneh Mokarram, Behnam Kadkhodaei, Hadi Ghasemi","doi":"10.1080/09553002.2024.2435320","DOIUrl":"10.1080/09553002.2024.2435320","url":null,"abstract":"<p><strong>Background: </strong>Resistance to chemo- and radiotherapy is the main obstacle in cancer treatment success, which results in cancer's poor prognosis. Therefore finding the exact mechanism of resistance may contribute to addressing this concern. This could result in improved cancer prognosis and survival outcomes for cancer patients by targeting the basic causes of resistance.</p><p><strong>Aim: </strong>This systematic review and meta-analysis assessed the potential of using autophagy-related proteins as prognostic biomarkers in radiotherapy-treated patients.</p><p><strong>Methods: </strong>Following PRISMA guidelines, we systematically reviewed 956 studies from PubMed, Scopus, and Web of Science databases until April 2023. The keywords used for this purpose were 'cancer', 'radiotherapy', 'prognosis', and 'Autophagy'. Then the related meta-analysis was performed using STATA software.</p><p><strong>Results: </strong>Four studies met the inclusion criteria. Upregulation of autophagy markers (LC3B, Beclin1 and ULK1) and subsequent activation of autophagy were significantly associated with a higher risk of mortality (1.95 times) in radiotherapy-treated groups compared with patients with low expression of these markers. Although such results were observed for recurrence-free survival (RFS); however, it was not significant.</p><p><strong>Conclusion: </strong>The findings of this meta-analysis suggest that autophagy activation may be a critical factor in resistance to radiotherapy and subsequent poor survival rates in cancer patients. Consequently, assessing the expression of autophagy-related markers like Beclin1, LC3II, P62, and ULK may be a useful method for monitoring cancer prognosis following radiotherapy.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"232-239"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142776008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichostatin A ameliorated combined radiation and skin wound injury-induced mortality and hematopoietic suppression in a rat model.","authors":"Niyati Dhingra, Varsha Gupta, Anuradha Tyagi, Paban K Agrawala, Vanita Gupta","doi":"10.1080/09553002.2025.2537211","DOIUrl":"10.1080/09553002.2025.2537211","url":null,"abstract":"<p><strong>Purpose: </strong>Effective countermeasure modalities against combined radiation injury, where the consequences of radiation exposure are highly complex with one or more conditions like burns, wounds, or infection, are yet far from reality. The current study aims to evaluate Trichostatin A (TSA), a presumptive radiomitigative molecule, in a rat model of combined radiation and wound injury (CRWI).</p><p><strong>Methods: </strong>Sprague-Dawley rats (200 ± 10 g) were whole-body exposed to different doses of ⁶⁰Co-γ-irradiation (4-7Gy) alone and with an excision wound (EW, size 20 ± 2 mm diameter), to optimize CRWI radiation dose. The efficacy of TSA (75 ng/kg b.w.) was evaluated at LD_50/30 CRWI dose, by administering intravenously in 2- and 5-doses, starting at 1 h post-irradiation and thereafter every 24 h to monitor 30-day survival, wound area, body weight, bacterial load, systemic changes, and histology.</p><p><strong>Results: </strong>CRWI radiation dose-response curve rendered 5 Gy as LD_50/30. Dose-dependent study of TSA showed that 5-doses and 2-doses rendered 83.3% and 50% survival respectively, against 50% survival after CRWI (5 Gy + EW). Treatment with TSA-5-doses resulted in 99% wound reduction (vs. 83% in CRWI); recovered CRWI-induced body weight loss, WBC, and platelets; ameliorated bacterial load in blood and skin wound; downregulated apoptosis (Bax expression) and cytotoxicity in bone marrow cells; reduced inflammation (TNF-α, IL-6, IL-1β), and normalized systemic histone deacetylase along-with restoration of structural changes in skin, bone marrow, and spleen.</p><p><strong>Conclusion: </strong>This study demonstrated that TSA substantially enhanced the survival of animals exposed to CRWI probably by ameliorating hematopoietic suppression and delayed wound healing.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"952-972"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144786251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of stromal neural crest progenitor cells on neuroblastoma radioresistance.","authors":"Carlos Huertas-Castaño, Laura Martínez-López, Patricia Cabrera-Roldán, Nuria Pastor, Juan Carlos Mateos, Santiago Mateos, Ricardo Pardal, Inmaculada Domínguez, Manuel Luis Orta","doi":"10.1080/09553002.2024.2440865","DOIUrl":"10.1080/09553002.2024.2440865","url":null,"abstract":"<p><strong>Purpose: </strong>A substantial proportion of children with high risk Neuroblastoma die within the first 5 years post-diagnosis despite the complex treatment applied. In the recent years, tumor environment has been revealed as key factor for cancer treatment efficacy. In this sense, non-tumorigenic Neural Crest progenitor cells from high risk patients, have been described as part of Neuroblastoma stroma, promoting tumor growth and contributing to mesenchyme formation. In this paper we wanted to study the radiobiological behavior of these cells (NB14t) and how they influence the growth of tumorigenic neuroblasts after radiotherapy.</p><p><strong>Materials and methods: </strong>To achieve our aim, we employed a wide list of methods either using NB14t cells as well as commercial NB cells. We have analyzed viability, survival, cell cyle profiles and differentiation. In addition, cocultured experiments were performed to monitor the influence of stroma cells to tumorigenic neuroblasts.</p><p><strong>Results: </strong>We found that stromal progenitor cells showed an extraordinary radio-resistance either cultured in attached or suspension conditions. In good agreement, we found an enhanced repair of irradiation-induced DNA lesions as compared with commercial cell lines. In addition, according to our data these cells differentiate into a Cancer Associated Fibroblasts (CAFs)-like phenotype, hence contributing to the formation of mesenchymal stroma enhancing the growth of tumor cells after irradiation.</p><p><strong>Conclusion: </strong>Our data show that neural progenitor cells from high risk NB stroma are radio-resistant and promote cancer growth after irradiation. This paper can help to understand the complex cell relationships within a tumor that will determine patient prognosis after radiotherapy.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"153-163"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Down-regulation of PCK2 enhanced the radioresistance phenotype of nasopharyngeal carcinoma.","authors":"Yijun He, Li Yan, Ruiqi Zhang, Rui Yang, Zhaolu Kong, Xiaosheng Wang","doi":"10.1080/09553002.2025.2470226","DOIUrl":"10.1080/09553002.2025.2470226","url":null,"abstract":"<p><strong>Purpose: </strong>Radiotherapy is the main treatment for non-distant metastatic nasopharyngeal carcinoma (NPC). However, about 15% of NPC patients still experience local tumor recurrence after radiotherapy. The mechanisms underlying the radioresistance of NPC have not been fully understood. This study explored the role of phosphoenolpyruvate carboxykinase2 (PCK2) in NPC radiosensitivity.</p><p><strong>Methods: </strong>Two NPC cell lines, SUNE-1 and 5-8 F, were enrolled in this research, and their radioresistant counterparts, SUNE-1R and 5-8FR were obtained by long-term exposure to γ-ray (2 Gy*30 times). Cells response to ionizing radiation (IR) was determined by colony formation assay (C.F.A). Immunofluorescence, microsphere formation assay and ferroptosis test were adopted to compare the difference of DNA damage repair, stemness and cell death between sensitive and resistant NPC cells. The biological functions of PCK2 were investigated by knocking down the endogenous PCK2 in vitro and in vivo. In addition, the tumor morphology and related markers were observed by HE staining and immunohistochemistry assay, respectively.</p><p><strong>Results: </strong>Intracellular PCK2 is transiently upregulated after a single irradiation in both SUNE-1 and 5-8 F cells. However, PCK2 was significantly reduced in radiation-tolerant cells that survived from multiple irradiations. Further work showed that PCK2 down-regulation prevented SUNE-1R and 5-8FR cells from IR-induced ferroptosis, accompanied by increased cell ability of DNA damaged repair and enhanced phenotypes of tumor stem cells.</p><p><strong>Conclusions: </strong>Our findings indicate that low expression of PCK2 is an important phenotypic feature of radioresistant NPC cells. It could be a result of PCK2 involved in regulating radiation-induced ferroptosis. Regulating the expression of PCK2 may provide new strategies for improving NPC radiation sensitivity.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"499-509"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IEPA, a novel radiation countermeasure, alleviates acute radiation syndrome in rodents.","authors":"Radoslaw Wesolowski, Brian L Fish, Michael Eibl, Stella Bähr, Srishti Munjal Mehta, Maciej T Czajkowski, Tracy Gasperetti, Christie M Orschell, Corinna Asang, Nikita Singh, Heather A Himburg, Dirk Pleimes","doi":"10.1080/09553002.2024.2425312","DOIUrl":"10.1080/09553002.2024.2425312","url":null,"abstract":"<p><p>Repurposing therapeutic agents with existing clinical data is a common strategy for developing radiation countermeasures. IEPA (imidazolyl ethanamide pentandioic acid) is an orally bioavailable small molecule pseudopeptide with myeloprotective properties, a good clinical safety profile, and stable chemical characteristics facilitating stockpiling. Here, we evaluated IEPA's radiomitigative efficacy in the hematopoietic subsyndrome of acute radiation syndrome (H-ARS) using total-body irradiation (TBI) models in C57BL/6J mice and WAG/RijCmcr rats, applying various posology schemes and introducing syringe feeding of the IEPA formulation in the pudding. Additionally, we assessed IEPA in the delayed effects of acute radiation exposure (DEARE) model after partial-body irradiation (PBI) in WAG/RijCmcr rats. Endpoints included survival, body weight, hematology, and pulmonary parameters, depending on the model. Results from mouse and rat TBI models demonstrated survival improvements with repeated IEPA dosing at 10 mg/kg, with the largest benefits observed in the bi-daily (BID) treatment over the 30-day ARS phase in female rats. Survival across PBI-DEARE subsyndromes was comparable between IEPA and vehicle groups, though IEPA improved pulmonary parameters in female rats during the lung-DEARE phase. Sex-related differences in response to irradiation and IEPA were noted, with females showing a survival advantage. IEPA treatment is compatible with Neulasta® (Pegfilgrastim; PEG-G-CSF); adequately powered studies are needed to confirm the trend toward improved survival over standard care alone. IEPA is a promising development candidate as a medical countermeasure against the effects of acute radiation syndrome. Further confirmatory studies in small and large animal models should validate the robustness and translatability of preliminary rodent data on IEPA's radiomitigative efficacy.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prohibitin2 knockdown decreases glioma malignant phenotypes and radio-resistance by inhibiting mitophagy.","authors":"Xuefei Xue, Huiling Tan, Xingning Jiang, Jingfan Lu, Ting Sun, Wei Yang","doi":"10.1080/09553002.2025.2470203","DOIUrl":"10.1080/09553002.2025.2470203","url":null,"abstract":"<p><strong>Purpose: </strong>Prohibitin2 (PHB2), located in inner mitochondrial membrane (IMM), is an important receptor to induce mitophagy. PHB2 was identified as a cancer-promoting factor in most cancers. However, the function of PHB2 in glioma cells remains unclear. This study delved into the impact of PHB2 knockdown on the phenotype, radiosensitivity and mitophagy of glioma cells.</p><p><strong>Methods: </strong>PHB2 expression and its clinical relevance in glioma were investigated by western blot, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and TCGA databases. The malignant phenotypes of glioma cells were analyzed in vitro using cell proliferation, cell cycle, wound healing and transwell assay. The radiosensitivity of glioma cells was detected by colony forming assay. The potential mechanism by which PHB2 regulated mitophagy was investigated by coimmunoprecipitation assay.</p><p><strong>Results: </strong>The expression of PHB2 was significantly upregulated in glioma cells and closely correlated with the malignant degree of glioma. The knockdown of PHB2 inhibited the proliferation, migration and invasion activities of glioma cells. Furthermore, PHB2 knockdown enhanced the radiosensitivity of normoxic and hypoxic glioma cells and suppressed the ionizing radiation-induced mitophagy in glioma cells. Cyanide 3-chlorophenylhydrazone (CCCP), a mitophagy agonist, could reverse the phenotypes and radiosensitivity changes elicited by PHB2 knockdown. Additionally, PHB2 regulated the expression of PGAM5 and PINK1 by directly binding to PARL.</p><p><strong>Conclusions: </strong>Our findings revealed that PHB2 knockdown decreased glioma malignant phenotypes and radio-resistance by inhibiting mitophagy <i>via</i> PARL-PGAM5-PINK1-Parkin pathway. PHB2 is a promising candidate target for the development of new therapeutic strategy to enhance the efficacy of radiotherapy for glioma.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"487-498"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143545339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidences of radioresistance in <i>Drosophila melanogaster</i> from Northeastern Brazil.","authors":"Maria Gislaine Pereira, Erima Maria de Amorim, Aleson Aparecido da Silva, Danubia Guimarães-Silva, Andre Morgado Esteves, Claudia Rohde","doi":"10.1080/09553002.2024.2440858","DOIUrl":"10.1080/09553002.2024.2440858","url":null,"abstract":"<p><strong>Background: </strong>Ionizing radiation can inflict cellular damage, the severity of which is determined by the dose, exposure duration, and its capacity to penetrate cells. Some studies have demonstrated that genetic and epigenetic mechanisms have enabled organisms to develop adaptive traits and enhance their ability to repair DNA damage. Northeastern Brazil, a region containing rocky outcrops rich in uranium and thorium, is an ideal scenario to study natural radiation and its effects on natural populations. This study presents evidence of radioresistance in the offspring of a natural strain of <i>Drosophila melanogaster</i> resident in the municipality of Cerro Corá (CC-res), an environment with high levels of radon-222.</p><p><strong>Material and methods: </strong>Genotoxicity was assessed using the comet assay in offspring of the CC-res and Oregon-R (OR), the control group, both reared under the same laboratory conditions for between 7 and 13 months. The adults and their offspring larvae were exposed to the Cerro Corá environment for 6 days during the dry and wet seasons. Low damage index and frequency were observed only in the CC-res. To confirm the radioresistance, the same strains were exposed after 16 months of cultivation to controlled doses of gamma radiation.</p><p><strong>Results and conclusions: </strong>CC-res exhibited significantly lower levels of damage compared to the OR strain, with a clear dose-response effect to the irradiation observed exclusively in the OR group. The results support the occurrence of radioresistance in the CC-res strain and underscore the need for further in vivo studies investigations into the impact of Brazil's natural environmental radiation.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"164-173"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collaborative activities in a biological dosimetry network for radiation emergencies in South Korea.","authors":"Yang Hee Lee, Su San Yang, Hyo Jin Yoon, Hwa Young Kim, Soon Woo Kwon, Soo Kyung Jeong, Su Jung Oh, Seong-Hoon Park, Younghyun Lee, Ki Moon Seong","doi":"10.1080/09553002.2024.2447506","DOIUrl":"10.1080/09553002.2024.2447506","url":null,"abstract":"<p><strong>Purpose: </strong>Biological dosimetry is an essential analytic method to estimate the absorbed radiation dose in the human body by measuring changes in biomolecules after radiation exposure. Joint response in a network to mass-casualty radiation incidents is one way to overcome the limitations of biological dosimetry, sharing the workload among laboratories. This study aimed to investigate the current performance, collaborative activities and technical advances of the Korea biodosimetry network (K-BioDos), and suggest the future directions toward successful joint response.</p><p><strong>Materials and methods: </strong>A survey was performed to investigate the capacities of each laboratory and their expectations for the K-BioDos network. We summarized the capacities, expectations and technical advances of K-BioDos members. Based on the results, in-depth discussion was carried out to determine the future plan and activities of K-BioDos.</p><p><strong>Results: </strong>K-BioDos has grown to six laboratories since its establishment with three functional laboratories of biological dosimetry in South Korea. We constructed long-term strategy according the survey results, and performed various activities for enhanced biological dosimetry capabilities - including intercomparison exercises, education, and resource sharing. Through these active collaborations we achieved harmonization of biodosimetry protocols and technical improvement such as better image quality.</p><p><strong>Conclusions: </strong>K-BioDos network performed various activities for joint response and constructed long-term plans, considering the expectations and feedbacks of members. K-BioDos continue to support members to establish and develop biodosimetry tools. These efforts and findings could serve as a fundamental guide for coordinated network responses in the event of large-scale radiological disaster.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"274-282"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized combination methods for exploring novel space environment-responsive genes and their roles: insights from space-flown <i>C. elegans</i> and their implications for astronauts.","authors":"Zejun Li, Lei Zhao, Ge Zhang, Dong Mi, Yeqing Sun","doi":"10.1080/09553002.2025.2519561","DOIUrl":"10.1080/09553002.2025.2519561","url":null,"abstract":"<p><strong>Purpose: </strong>By expanding the catalog of spaceflight-induced molecular signatures in <i>Caenorhabditis elegans</i>, we aim to identify key molecular features and potential mechanisms underlying space environment-induced health risks to astronauts using <i>C. elegans</i> as a model organism.</p><p><strong>Methods and materials: </strong>We employed an optimized combination algorithm that integrated two co-expression network analysis methods and four machine learning-based models to identify space environment-responsive genes (SEGs) in space-flown <i>C. elegans</i>. The expression levels and associated biological processes of human orthologues of identified <i>C. elegans</i> genes were further analyzed using data from the JAXA CFE and NASA Twins studies.</p><p><strong>Results: </strong>A total of 114 SEGs that were implicated in four biological processes, including DNA repair, metabolic process, reproductive and developmental process, and lifespan regulation in space-flown <i>C. elegans</i>. We obtained 19 SEGs as potential indicators associated with health risks of the space environment. Further, the human orthologues of <i>C. elegans</i> SEGs that also exhibited altered expression in the blood samples of astronauts.</p><p><strong>Conclusions: </strong>This study provides new insights into the molecular mechanisms behind spaceflight-induced health risks and highlights potential mechanistic targets for preventive measures. The findings suggest a conserved genetic response to space conditions between <i>C. elegans</i> and astronauts, offering valuable targets for mitigating the health risks of space exploration.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"891-911"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of radiation-induced xerostomia: cross-cultural adaptation and psychometric properties of the Persian version of the Xerostomia Questionnaire (P-XQ) among older head and neck cancer patients.","authors":"Tahereh Molania Jolodar, Hadi Najafi, Amir Hossein Yousefi, Abdolhakim Jorbonyan, Shahab Papi","doi":"10.1080/09553002.2025.2537205","DOIUrl":"10.1080/09553002.2025.2537205","url":null,"abstract":"<p><strong>Background and purpose: </strong>Dry mouth (xerostomia) is one of the most common early and late side effects of Head and Neck Cancer (HNC) survivors, despite advances in radiotherapy planning and administration. Given the major influence of xerostomia on cancer patients' quality of life, this study examined the psychometric features of the Persian version of the Xerostomia Questionnaire (P-XQ) in older Iranian HNC patients.</p><p><strong>Methods: </strong>Conducted between April and June 2024, this methodological study used convenience sampling at the Iran Cancer Institute in Tehran, Iran. The P-XQ was translated, culturally adapted, and its psychometric properties were analyzed using SPSS v.25, with statistical significance set at p < .05.</p><p><strong>Results: </strong>A total of 200 older patients aged 60 or older, participated in this study. Face, content (SCVI/average = 0.89) and cross-cultural validity were established by the experts. Confirmatory factor analysis demonstrated that the extracted model was a good fit (GFI = 0.91 and CFI = 0.95). The Pearson correlation between P-SDS and the Xerostomia Inventory (XI) questionnaire was significant (<i>p</i> < 0.05, r = 0.92). The internal consistency (Cronbach's α = 0.93) and test-retest reliability (ICC = 0.92) of the Persian version (P-XQ) were both satisfactory. Except for gender and smoking, other variables showed significant relationships with the P-XQ scores. No floor or ceiling effects were found.</p><p><strong>Conclusion: </strong>Researchers can utilize the P-XQ to measure radiation-induced xerostomia in older HNC patients due to its excellent reliability, validity, and ease of use.</p>","PeriodicalId":94057,"journal":{"name":"International journal of radiation biology","volume":" ","pages":"942-951"},"PeriodicalIF":2.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}